Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for +publisher:"Vanderbilt University" +contributor:("Dr. Todd Giorgio"). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Vanderbilt University

1. Hoang, Anh Ngoc. Hemozoin: a case of heme crystal engineering.

Degree: PhD, Interdisciplinary Materials Science, 2010, Vanderbilt University

During the pathogenic blood stage of a malaria infection, the Plasmodium parasites degrade hemoglobin as a source of nutrition. As a consequence, free heme, known to be toxic to the parasite, is released. It is believed that the parasite circumvents heme toxicity by sequestering the heme molecules into a dark brown crystalline material known as hemozoin. The molecular details associated with the formation of hemozoin and its synthetic counterpart, beta-hematin, are presented in this dissertation. Firstly, the biological mediator of hemozoin formation was investigated. Neutral lipid droplets (NLDs) were shown to be sufficient at mediating the production of brown pigments that are morphologically and chemically identical to hemozoin. Optimal partitioning of heme into NLDs was pH dependent with maximal heme conversion at a pH condition similar to that of the parasite√Ęs digestive food vacuole, the biological site of crystallization. The rate of beta-hematin formation was rapid enough to protect the parasite from heme toxicity. Secondly, the interfacial interactions between lipid molecules and heme were investigated using Langmuir-Blodgett monolayer creation techniques. Comparisons of these surface pressure-area isotherms revealed that the biological composition of neutral lipid is characterized by disordered packing of lipids. This fluid lipid surface may account for the low activation energy measured for beta-hematin formation associated with NLDs. Substituted protoporphyrin IX compression studies suggest that hemozoin nucleation begins when the propionic group of a heme unit anchors to the polar head group of the lipid molecules. Thirdly, crystallization parameters associated with beta-hematin formation was examined using various solvent conditions to facilitate heme solubility. The formation of beta-hematin using the aprotic solvent dimethylsulfoxide and some polyethyleneglycols demonstrates that crystallization is accelerated by increasing heme solubilization in acidic conditions, resulting on increased dispersion of amorphous heme precipitates. Crystallization data support the notion that modulation of the water activity is important mechanism to support spontaneous heme crystallization. Futhermore, through proper manipulation of solvent properties, the morphologies of beta-hematin can be controlled. Finally, beta-hematin crystals were applied to phage display technologies to identify short peptide sequences that specifically recognize select crystal face. Isolated peptides were sufficient at mediating beta-hematin formation. Advisors/Committee Members: Dr. Kane Jennings (committee member), Dr. Todd Giorgio (committee member), Dr. David Cliffel (chair), Dr. David Wright (chair), Dr. Rick Haselton (committee member).

Subjects/Keywords: hemozoin; neutral lipid; malaria

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hoang, A. N. (2010). Hemozoin: a case of heme crystal engineering. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://etd.library.vanderbilt.edu//available/etd-07272010-151453/ ;

Chicago Manual of Style (16th Edition):

Hoang, Anh Ngoc. “Hemozoin: a case of heme crystal engineering.” 2010. Doctoral Dissertation, Vanderbilt University. Accessed December 14, 2019. http://etd.library.vanderbilt.edu//available/etd-07272010-151453/ ;.

MLA Handbook (7th Edition):

Hoang, Anh Ngoc. “Hemozoin: a case of heme crystal engineering.” 2010. Web. 14 Dec 2019.

Vancouver:

Hoang AN. Hemozoin: a case of heme crystal engineering. [Internet] [Doctoral dissertation]. Vanderbilt University; 2010. [cited 2019 Dec 14]. Available from: http://etd.library.vanderbilt.edu//available/etd-07272010-151453/ ;.

Council of Science Editors:

Hoang AN. Hemozoin: a case of heme crystal engineering. [Doctoral Dissertation]. Vanderbilt University; 2010. Available from: http://etd.library.vanderbilt.edu//available/etd-07272010-151453/ ;


Vanderbilt University

2. Lee, Sue Hyun. Engineering Biomaterials-based Approaches for Better Angiogenesis.

Degree: PhD, Biomedical Engineering, 2016, Vanderbilt University

Tissue engineering promises to solve the ever-increasing organ donor shortage, as well as to provide personalized and customized cures for numerous life-threatening diseases and organ/tissue failures. While significant advances have been made in recent years, most tissue engineering applications face a common roadblock that holds them back from being translated in the clinic: the inability to engineer constructs that would support sufficient and rapid blood vessel formation (angiogenesis) upon implantation. Most tissues cannot survive nor function properly without elaborate blood vessel networks in place. Thus, the goal of this work is to modify and examine two commonly used biomaterials, polycaprolactone (PCL) and gelatin, that would enhance blood vessel formation in vitro and in vivo through different mechanisms. The first approach was to incorporate reactive oxygen species (ROS)-degradable peptide into PCL scaffolds that would allow better cell infiltration, which led to improved angiogenesis. In the second approach, by modifying gelatin to form a thermostable hydrogel, a novel interaction between gelatin hydrogel and mesenchymal stem cells (MSC) that drove MSC differentiation into blood vessel-forming endothelial cells was discovered and examined. This dissertation work is aimed at overcoming the common barrier for clinical translation of tissue engineering, and the findings and the resulting design principles can be applied in various tissue engineering applications to accelerate clinical translation. Advisors/Committee Members: Dr. Mark Does (chair), Dr. Todd Giorgio (committee member), Dr. Melissa Skala (committee member), Dr. Leon Bellan (committee member), Dr. David Bader (committee member).

Subjects/Keywords: Tissue Engineering; Stem Cell; Biomaterials

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Lee, S. H. (2016). Engineering Biomaterials-based Approaches for Better Angiogenesis. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://etd.library.vanderbilt.edu//available/etd-04112016-130804/ ;

Chicago Manual of Style (16th Edition):

Lee, Sue Hyun. “Engineering Biomaterials-based Approaches for Better Angiogenesis.” 2016. Doctoral Dissertation, Vanderbilt University. Accessed December 14, 2019. http://etd.library.vanderbilt.edu//available/etd-04112016-130804/ ;.

MLA Handbook (7th Edition):

Lee, Sue Hyun. “Engineering Biomaterials-based Approaches for Better Angiogenesis.” 2016. Web. 14 Dec 2019.

Vancouver:

Lee SH. Engineering Biomaterials-based Approaches for Better Angiogenesis. [Internet] [Doctoral dissertation]. Vanderbilt University; 2016. [cited 2019 Dec 14]. Available from: http://etd.library.vanderbilt.edu//available/etd-04112016-130804/ ;.

Council of Science Editors:

Lee SH. Engineering Biomaterials-based Approaches for Better Angiogenesis. [Doctoral Dissertation]. Vanderbilt University; 2016. Available from: http://etd.library.vanderbilt.edu//available/etd-04112016-130804/ ;

.