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Vanderbilt University
1.
Deel, Megan Elizabeth.
Alterations in GABAA receptor expression and physiology in a mouse model of idiopathic generalized epilepsy.
Degree: MS, Neuroscience, 2012, Vanderbilt University
URL: http://hdl.handle.net/1803/14874 
► The proper function of the nervous system is dependent upon a delicate balance between excitatory and inhibitory activity in the brain. GABAA receptors are extremely…
(more)
▼ The proper function of the nervous system is dependent upon a delicate balance between excitatory and inhibitory activity in the brain. GABAA receptors are extremely important in the maintenance of this balance because they mediate the majority of fast inhibition in the adult central nervous system. Several genetic mutations in various human GABAA receptor subunits have been associated with idiopathic generalized epilepsy syndromes. Here we have investigated the consequences of GABAA receptor dysfunction using a mouse model based on one of the aforementioned genetic mutations identified in a human epilepsy patient. We begin with a general introduction to epilepsy followed by a more detailed discussion of the particular epilepsy syndrome studied herein. We then provide a thorough review of GABAA receptor structure and function and highlight previous findings related to our current studies. We then provide an explanation of the rationale and general experimental strategy employed in our studies. Next we provide the specifics of our methodology proceeded by the presentation of our results. In the final chapter we discuss our interpretation of the data and its implications for the advancement of our understanding of epilepsy.
Advisors/Committee Members: Douglas G. McMahon (committee member), Martin J. Gallagher (Committee Chair).
Subjects/Keywords: childhood absence epilepsy; seizure; epilepsy; GABA; compensation
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APA (6th Edition):
Deel, M. E. (2012). Alterations in GABAA receptor expression and physiology in a mouse model of idiopathic generalized epilepsy. (Thesis). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14874
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Deel, Megan Elizabeth. “Alterations in GABAA receptor expression and physiology in a mouse model of idiopathic generalized epilepsy.” 2012. Thesis, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/14874.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Deel, Megan Elizabeth. “Alterations in GABAA receptor expression and physiology in a mouse model of idiopathic generalized epilepsy.” 2012. Web. 23 Jan 2021.
Vancouver:
Deel ME. Alterations in GABAA receptor expression and physiology in a mouse model of idiopathic generalized epilepsy. [Internet] [Thesis]. Vanderbilt University; 2012. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/14874.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Deel ME. Alterations in GABAA receptor expression and physiology in a mouse model of idiopathic generalized epilepsy. [Thesis]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/14874
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Vanderbilt University
2.
Olena, Abigail Frances.
microRNA Regulation of Zebrafish Retinal Development.
Degree: PhD, Biological Sciences, 2015, Vanderbilt University
URL: http://hdl.handle.net/1803/10500
► microRNAs (miRNAs) are small noncoding RNAs that bind the 3’ untranslated regions (UTRs) of mRNA targets and, acting with associated proteins, facilitate translation repression and…
(more)
▼ microRNAs (miRNAs) are small noncoding RNAs that bind the 3’ untranslated regions (UTRs) of mRNA targets and, acting with associated proteins, facilitate translation repression and degradation of target mRNAs. Since their discovery in C. elegans, miRNAs and their accessory proteins have been shown to be conserved throughout phylogeny. miRNAs exert their regulatory functions in myriad biological settings, from development and growth to disease. What follows is a general overview of miRNA discovery, organization, and function, and of the use of the powerful vertebrate developmental biology model organism, zebrafish (Danio rerio), zebrafish retinal development, and an overview of Notch-Delta signaling. The purpose of this dissertation is to examine the role of a specific miRNA, miR-216a, in regulation of the Notch-Delta signaling pathway during zebrafish retinal development.
Advisors/Committee Members: James G. Patton (committee member), John S. Penn (committee member), Douglas G. McMahon (Committee Chair).
Subjects/Keywords: miR-216a; Notch signaling; microRNA; sorting nexin 5; zebrafish; retina
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APA (6th Edition):
Olena, A. F. (2015). microRNA Regulation of Zebrafish Retinal Development. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10500
Chicago Manual of Style (16th Edition):
Olena, Abigail Frances. “microRNA Regulation of Zebrafish Retinal Development.” 2015. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/10500.
MLA Handbook (7th Edition):
Olena, Abigail Frances. “microRNA Regulation of Zebrafish Retinal Development.” 2015. Web. 23 Jan 2021.
Vancouver:
Olena AF. microRNA Regulation of Zebrafish Retinal Development. [Internet] [Doctoral dissertation]. Vanderbilt University; 2015. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/10500.
Council of Science Editors:
Olena AF. microRNA Regulation of Zebrafish Retinal Development. [Doctoral Dissertation]. Vanderbilt University; 2015. Available from: http://hdl.handle.net/1803/10500
Vanderbilt University
3.
Khuansuwan, Sataree.
A transcription factor network dictates neuronal cell fate decisions in the zebrafish dorsal diencephalon.
Degree: PhD, Biological Sciences, 2014, Vanderbilt University
URL: http://hdl.handle.net/1803/14223
► The zebrafish epithalamus, which includes the dorsal habenular nuclei and pineal complex, displays robust molecular and anatomical asymmetries. The full elaboration of molecular and anatomical…
(more)
▼ The zebrafish epithalamus, which includes the dorsal habenular nuclei and pineal complex, displays robust molecular and anatomical asymmetries. The full elaboration of molecular and anatomical differences between the left and right dorsal habenular nuclei depends on the formation of a left-sided parapineal organ. This work has yielded significant insights into the role of Tbx2b during parapineal development using both the candidate gene approach as well as the non-biased, transcriptome analysis approach. Through the candidate approach, it was determined that Tbx2b acts in a permissive manner during parapineal specification, but it is necessary for proper parapineal migration. Further, the inhibitory roles of Flh and Nr2e3 during formation of parapineal neurons have been characterized. Using RNA-sequencing to perform differential expression analysis between wild-type and tbx2b-deficient pineal complex cells has yielded many targets that are downstream of Tbx2b. Together, these studies also provided a novel insight into a potential genetic interaction system between Tbx2b and Nr2e3 during cone versus rod photoreceptor differentiation.
Advisors/Committee Members: Wenbiao Chen (committee member), Joshua T. Gamse (committee member), Douglas G. McMahon (committee member), Kevin Ess (committee member), James G. Patton (Committee Chair).
Subjects/Keywords: parapineal; left-right asymmetry; Danio rerio; zebrafish; Tbx2b; pineal; pineal complex; transcriptome analysis; Flh; Nr2e3; FACS
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APA (6th Edition):
Khuansuwan, S. (2014). A transcription factor network dictates neuronal cell fate decisions in the zebrafish dorsal diencephalon. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14223
Chicago Manual of Style (16th Edition):
Khuansuwan, Sataree. “A transcription factor network dictates neuronal cell fate decisions in the zebrafish dorsal diencephalon.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/14223.
MLA Handbook (7th Edition):
Khuansuwan, Sataree. “A transcription factor network dictates neuronal cell fate decisions in the zebrafish dorsal diencephalon.” 2014. Web. 23 Jan 2021.
Vancouver:
Khuansuwan S. A transcription factor network dictates neuronal cell fate decisions in the zebrafish dorsal diencephalon. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/14223.
Council of Science Editors:
Khuansuwan S. A transcription factor network dictates neuronal cell fate decisions in the zebrafish dorsal diencephalon. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/14223
Vanderbilt University
4.
Shen, Dingding.
Characterization of GABAA receptor subunit mutations associated with epileptic encephalopathies.
Degree: PhD, Neuroscience, 2017, Vanderbilt University
URL: http://hdl.handle.net/1803/14271
► Epileptic encephalopathies (EEs) are a devastating group of severe childhood onset epilepsies with medication resistant seizures and poor developmental outcomes. Many EEs have a genetic…
(more)
▼ Epileptic encephalopathies (EEs) are a devastating group of severe childhood onset epilepsies with medication resistant seizures and poor developmental outcomes. Many EEs have a genetic etiology and are often associated with de novo mutations in genes coding for proteins involved in synaptic transmission, including GABAA receptor subunit genes. A better understanding of GABAA receptor subunit mutations associated with EEs in vitro and in vivo will facilitate epilepsy diagnosis as well as treatments in the future. Here we employed a combination of next generation sequencing and in vitro functional assays and established for the first time that missense GABRG2 mutations are genetic risk factors for EEs. In addition, we focused on three nonsense GABRG2 mutations associated with epilepsies of different severities and demonstrated that they resulted in different structural disturbance and different suppression of wild-type partnering subunits. Finally we investigated the performance of heterozygous knock-in (KI) mice which bear the GABRB3(N110D) mutation associated with infantile spasms (Gabrb3+/N110D KI mice) in a battery of behavioral tasks, showing that they had significantly abnormal neurobehavioral profiles persisting into adulthood. To conclude, we have shown meaningful functional and structural changes for EE-associated GABRG2 mutations in vitro, and have determined the behavioral comorbidities of KI mice harboring a human infantile spasms GABRB3 mutation in vivo.
Advisors/Committee Members: Kevin C. Ess (committee member), Robert L. Macdonald (committee member), Martin J. Gallagher (committee member), Douglas G. McMahon (Committee Chair).
Subjects/Keywords: Epileptic encephalopathies; GABAA receptor
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APA (6th Edition):
Shen, D. (2017). Characterization of GABAA receptor subunit mutations associated with epileptic encephalopathies. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14271
Chicago Manual of Style (16th Edition):
Shen, Dingding. “Characterization of GABAA receptor subunit mutations associated with epileptic encephalopathies.” 2017. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/14271.
MLA Handbook (7th Edition):
Shen, Dingding. “Characterization of GABAA receptor subunit mutations associated with epileptic encephalopathies.” 2017. Web. 23 Jan 2021.
Vancouver:
Shen D. Characterization of GABAA receptor subunit mutations associated with epileptic encephalopathies. [Internet] [Doctoral dissertation]. Vanderbilt University; 2017. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/14271.
Council of Science Editors:
Shen D. Characterization of GABAA receptor subunit mutations associated with epileptic encephalopathies. [Doctoral Dissertation]. Vanderbilt University; 2017. Available from: http://hdl.handle.net/1803/14271
Vanderbilt University
5.
Bretz, Colin Andrew.
The Role of NFAT Family Transcription Factors in Diabetic Retinopathy.
Degree: PhD, Cell and Developmental Biology, 2015, Vanderbilt University
URL: http://hdl.handle.net/1803/10754
► Diabetic retinopathy (DR) is one of the leading causes of vision loss in working age populations. Clinically, DR is divided into early non-proliferative diabetic retinopathy…
(more)
▼ Diabetic retinopathy (DR) is one of the leading causes of vision loss in working age populations. Clinically, DR is divided into early non-proliferative diabetic retinopathy (NPDR), characterized by retinal vascular inflammation, hyperpermeability, and leukostasis; and late stage proliferative diabetic retinopathy (PDR), characterized by focal ischemia and neovascularization. This work evaluates the role of NFAT family transcription factors in both NPDR and PDR pathologies.
TNFα, an inflammatory cytokine elevated in the vitreous of patients with DR, is implicated in the development of retinal vascular inflammation and leukostasis in NPDR. RNA-seq analysis using human retinal microvascular endothelial cells (HRMEC) treated with TNFα and a NFAT inhibitor revealed a role for NFAT signaling in the expression of leukocyte recruitment and adhesion proteins; and inhibition in both in vitro and in vivo models of leukocyte adhesion confirmed a clear role for NFAT signaling in TNFα-induced retinal leukostasis. Subsequent studies using isoform-specific siRNA evaluated the role of individual NFAT isoforms in this context, and identified distinct and sometimes counteractive roles for each. Specifically, NFATc2 and NFATc4 were found to regulate TNFα-induced leukocyte adhesion proteins and chemoattractants respectively, and knockdown of either isoform reduced leukocyte adhesion to HRMEC.
VEGF is another important signaling peptide in the development of DR pathology, and has been identified as an inducer of NFAT signaling in certain cell types, though whether this also occurred in HRMEC or retinal vasculature was unclear. In vitro analysis showed that VEGF stimulates NFATc1 nuclear translocation in HRMEC, and that NFAT inhibition blocks proliferation and tube formation. Subsequent in vivo studies using the oxygen-induced retinopathy model showed that treatment with NFAT inhibitors significantly reduced retinal neovascularization.
Taken together, these studies indicate that NFAT signaling contributes to both NPDR and PDR pathology, and highlight the unique roles individual NFAT isoforms play in responding to disease-relevant stimuli. Targeting individual NFAT isoforms may prove to be a valuable and effective way to target particular aspects of DR pathology, and further investigation of these transcription factors will help develop a more complete understanding of the molecular mechanisms that underlie DR.
Advisors/Committee Members: David M Miller (committee member), Douglas G McMahon (committee member), John S Penn (committee member), Chin Chiang (Committee Chair).
Subjects/Keywords: retinal inflammation; NFAT; diabetic retinopathy; retinal leukostasis; angiogenesis
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APA (6th Edition):
Bretz, C. A. (2015). The Role of NFAT Family Transcription Factors in Diabetic Retinopathy. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10754
Chicago Manual of Style (16th Edition):
Bretz, Colin Andrew. “The Role of NFAT Family Transcription Factors in Diabetic Retinopathy.” 2015. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/10754.
MLA Handbook (7th Edition):
Bretz, Colin Andrew. “The Role of NFAT Family Transcription Factors in Diabetic Retinopathy.” 2015. Web. 23 Jan 2021.
Vancouver:
Bretz CA. The Role of NFAT Family Transcription Factors in Diabetic Retinopathy. [Internet] [Doctoral dissertation]. Vanderbilt University; 2015. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/10754.
Council of Science Editors:
Bretz CA. The Role of NFAT Family Transcription Factors in Diabetic Retinopathy. [Doctoral Dissertation]. Vanderbilt University; 2015. Available from: http://hdl.handle.net/1803/10754
Vanderbilt University
6.
Kara, Nergis.
microRNA Function in Zebrafish Development and Regeneration.
Degree: PhD, Biological Sciences, 2018, Vanderbilt University
URL: http://hdl.handle.net/1803/14353
► microRNAs (miRNAs) are a family of highly conserved small noncoding RNAs that post transcriptionally regulate gene expression and play important roles in many cellular processes…
(more)
▼ microRNAs (miRNAs) are a family of highly conserved small noncoding RNAs that post transcriptionally regulate gene expression and play important roles in many cellular processes during development and regeneration. The work presented in this thesis focuses on discovering functions of miRNAs in craniofacial development and retina regeneration in zebrafish. Chapter II describes the role of miR-27 in regulating chondrogenesis during pharyngeal morphogenesis. Chondrogenesis in the pharyngeal arches involves differentiation of cranial neural crest cells into chondrocytes, tightly regulated by temporal intracellular and extracellular cues. miR-27 is highly enriched in the pharyngeal arches and acts as a positive regulator of chondrogenesis by controlling the activity of Focal Adhesion Complex. Chapter III focuses on retina regeneration in adult zebrafish and describes how miR-216 regulates the initiation of Müller glia (MG) dependent retina regeneration. Unlike the adult mammalian retina, MG in the adult zebrafish retina are able to dedifferentiate into a ‘stem cell’-like state and give rise to multipotent progenitor cells upon retinal damage. miR-216a is downregulated in MG after constant intense light lesioning allowing upregulation of the H3K79 methyltransferase Dot1l which is required for MG reprogramming and proliferation downstream of miR-216a. Chapter IV presents a genome-wide transcriptome analysis of MG dedifferentiation during retina regeneration serving as a resource for the identification of potential therapeutic targets.
Advisors/Committee Members: James G. Patton (committee member), John A. Capra (committee member), Wenbiao Chen (committee member), Douglas G. McMahon (Committee Chair).
Subjects/Keywords: zebrafish; microRNA; development; regeneration; retina; Müller glia
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APA (6th Edition):
Kara, N. (2018). microRNA Function in Zebrafish Development and Regeneration. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14353
Chicago Manual of Style (16th Edition):
Kara, Nergis. “microRNA Function in Zebrafish Development and Regeneration.” 2018. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/14353.
MLA Handbook (7th Edition):
Kara, Nergis. “microRNA Function in Zebrafish Development and Regeneration.” 2018. Web. 23 Jan 2021.
Vancouver:
Kara N. microRNA Function in Zebrafish Development and Regeneration. [Internet] [Doctoral dissertation]. Vanderbilt University; 2018. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/14353.
Council of Science Editors:
Kara N. microRNA Function in Zebrafish Development and Regeneration. [Doctoral Dissertation]. Vanderbilt University; 2018. Available from: http://hdl.handle.net/1803/14353
Vanderbilt University
7.
Arain, Fazal Manzoor.
Two Human Epilepsy Mutations Cause Developmentally Dependant Changes in Seizure Phenotype and GABAA Receptor Expression in Genetically Modified Mice.
Degree: PhD, Neuroscience, 2014, Vanderbilt University
URL: http://hdl.handle.net/1803/10463
► Epilepsy is a disease characterized by two or more unprovoked seizures. Two mutations, S326fs328X and A322D, in the α1 subunit of GABAA receptor (Gabra1), are…
(more)
▼ Epilepsy is a disease characterized by two or more unprovoked seizures. Two mutations, S326fs328X and A322D, in the α1 subunit of GABAA receptor (Gabra1), are associated with childhood absence epilepsy and juvenile myoclonic epilepsy, respectively. In vitro, S326fs328X caused complete, while A322D caused an 88% reduction in α1 subunit expression. Here, we showed that both Gabra1 knock-out (KO) and A322D knock-in (KI) mutations cause absence seizures in post-natal day 33-37 (P30s) genetically modified mice that persist at least until P120. In addition, at P120, both Gabra1 KO and A322D KI mice also had atypical polyspike discharges on electroencephalogram recordings that were occasionally associated with subtle, but visible, myoclonic-like jerks. Western blot experiments showed that the expression of α1 subunit decreased, while α3 subunit increased, in the cortex of both Gabra1 KO and A322D KI mice. Surprisingly, at P120, wild type mice had reduced α3 subunit expression, but it remained high in the mutants. Confocal fluorescent microscopy showed, that at P120, gephyrin, a marker of inhibitory synapses, co-localized with α3 subunit more in the mutants compared to wild types, indicating that there was substantially more synaptic α3 subunit in the mutants. These findings demonstrate the α1 subunit haploinsufficiency causes absence epilepsy and developmentally-dependent myoclonic epilepsy. In addition, because α3 expressing GABAA receptors have reduced GABA sensitivity and prolonged activation times, our results suggest that reduced GABAergic inhibition may contribute to the epilepsy phenotype in these mutant mice at this age.
Advisors/Committee Members: Nathalie Maitre (committee member), Robert L. Macdonald (committee member), Douglas G. McMahon (committee member), Martin J. Gallagher (committee member), Kevin Ess (Committee Chair).
Subjects/Keywords: GABA; Epilepsy; Development
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APA (6th Edition):
Arain, F. M. (2014). Two Human Epilepsy Mutations Cause Developmentally Dependant Changes in Seizure Phenotype and GABAA Receptor Expression in Genetically Modified Mice. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10463
Chicago Manual of Style (16th Edition):
Arain, Fazal Manzoor. “Two Human Epilepsy Mutations Cause Developmentally Dependant Changes in Seizure Phenotype and GABAA Receptor Expression in Genetically Modified Mice.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/10463.
MLA Handbook (7th Edition):
Arain, Fazal Manzoor. “Two Human Epilepsy Mutations Cause Developmentally Dependant Changes in Seizure Phenotype and GABAA Receptor Expression in Genetically Modified Mice.” 2014. Web. 23 Jan 2021.
Vancouver:
Arain FM. Two Human Epilepsy Mutations Cause Developmentally Dependant Changes in Seizure Phenotype and GABAA Receptor Expression in Genetically Modified Mice. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/10463.
Council of Science Editors:
Arain FM. Two Human Epilepsy Mutations Cause Developmentally Dependant Changes in Seizure Phenotype and GABAA Receptor Expression in Genetically Modified Mice. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/10463
Vanderbilt University
8.
Jones, Jeffrey Robert.
Linking molecular, electrical, and behavioral rhythms in the brain’s biological clock.
Degree: PhD, Neuroscience, 2015, Vanderbilt University
URL: http://hdl.handle.net/1803/10633
► Understanding the relationship between gene networks, neurons, and circuits that determine behavior is a fundamental problem in neuroscience. The brain’s biological clock – the suprachiasmatic…
(more)
▼ Understanding the relationship between gene networks, neurons, and circuits that determine behavior is a fundamental problem in neuroscience. The brain’s biological clock – the suprachiasmatic nucleus (SCN) – is an excellent model system in which to study this crucial problem. SCN neurons possess daily molecular transcriptional/translational feedback loops and exhibit rhythms in spontaneous action potential frequency. The synchronized output of the SCN neural network ultimately dictates circadian behavior and physiology. A key unsolved question in circadian neurobiology is how these rhythms interact to form a coherent pacemaker. To address this question, I combined electrophysiology, real-time imaging of gene expression, SCN-specific optogenetic manipulation of neuronal firing, and monitoring of locomotor activity to elucidate the links between the molecular and electrical rhythms that comprise the brain’s biological clock and their circadian behavioral output. I found that optogenetic induction or suppression of firing rate within SCN neurons is sufficient to reset the phase and alter the period of the molecular clockworks, that this resetting requires action potentials and network communication, and that in vivo optogenetic stimulation of the SCN entrains locomotor activity rhythms. Additionally, the expression of the clock gene Period1 is necessary for the coordination of molecular and electrical rhythms in SCN neurons. Thus, I conclude that there is a bidirectional relationship between circadian rhythms in gene expression and electrical activity in SCN neurons such that firing rate is both an output of and an input onto the molecular clock.
Advisors/Committee Members: Danny G. Winder (committee member), Christopher S. Colwell (committee member), Douglas G. McMahon (committee member), Terry L. Page (Committee Chair).
Subjects/Keywords: firing rate; bioluminescence; suprachiasmatic; optogenetics; circadian
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APA (6th Edition):
Jones, J. R. (2015). Linking molecular, electrical, and behavioral rhythms in the brain’s biological clock. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10633
Chicago Manual of Style (16th Edition):
Jones, Jeffrey Robert. “Linking molecular, electrical, and behavioral rhythms in the brain’s biological clock.” 2015. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/10633.
MLA Handbook (7th Edition):
Jones, Jeffrey Robert. “Linking molecular, electrical, and behavioral rhythms in the brain’s biological clock.” 2015. Web. 23 Jan 2021.
Vancouver:
Jones JR. Linking molecular, electrical, and behavioral rhythms in the brain’s biological clock. [Internet] [Doctoral dissertation]. Vanderbilt University; 2015. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/10633.
Council of Science Editors:
Jones JR. Linking molecular, electrical, and behavioral rhythms in the brain’s biological clock. [Doctoral Dissertation]. Vanderbilt University; 2015. Available from: http://hdl.handle.net/1803/10633
Vanderbilt University
9.
Dean, Benjamin Jurrien.
Neurogenic Determinants of Left-Right Brain Asymmetry: Developmental Investigations of the Zebrafish Habenular Nuclei.
Degree: PhD, Neuroscience, 2014, Vanderbilt University
URL: http://hdl.handle.net/1803/13815
► Left-Right asymmetry of the CNS is a highly conserved feature across vertebrate classes. Asymmetry is manifest at the levels of function and connectivity. But the…
(more)
▼ Left-Right asymmetry of the CNS is a highly conserved feature across vertebrate classes. Asymmetry is manifest at the levels of function and connectivity. But the neural correlates of these asymmetries and their developmental underpinnings are just beginning to be explored. The zebrafish habenular nuclei have functional as well as neuronal asymmetries and offer a molecularly manipulable and highly visualizable model to studying the cellular and developmental origins of CNS left-right asymmetry. Here I report an evolving developmental network that regulates the timing of habenular neurogenesis. This network involved environmental and endocrine cues (light and melatonin), morphogenetic pathways (FGF and Nodal) and several neurogenic genes (dbx1b, lhx9, kip2 and her6). These components act as a temporal ‘neurogenic gate,’ dictating the onset of neurogenesis. This neurogenic gate is regulated asymmetrically – acting first in the left habenula driving earlier neurogenesis there – and impacts neuronal cell type specification. Together these findings deepen our understanding of the molecular mechanisms that drive neural fate specification and how these components can give rise to left-right asymmetry in the CNS.
Advisors/Committee Members: Charles C. Hong (committee member), Joshua T. Gamse (committee member), Douglas G. McMahon (committee member), David M. Miller III (Committee Chair).
Subjects/Keywords: brain development; neurogenesis; Danio rerio
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APA (6th Edition):
Dean, B. J. (2014). Neurogenic Determinants of Left-Right Brain Asymmetry: Developmental Investigations of the Zebrafish Habenular Nuclei. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/13815
Chicago Manual of Style (16th Edition):
Dean, Benjamin Jurrien. “Neurogenic Determinants of Left-Right Brain Asymmetry: Developmental Investigations of the Zebrafish Habenular Nuclei.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/13815.
MLA Handbook (7th Edition):
Dean, Benjamin Jurrien. “Neurogenic Determinants of Left-Right Brain Asymmetry: Developmental Investigations of the Zebrafish Habenular Nuclei.” 2014. Web. 23 Jan 2021.
Vancouver:
Dean BJ. Neurogenic Determinants of Left-Right Brain Asymmetry: Developmental Investigations of the Zebrafish Habenular Nuclei. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/13815.
Council of Science Editors:
Dean BJ. Neurogenic Determinants of Left-Right Brain Asymmetry: Developmental Investigations of the Zebrafish Habenular Nuclei. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/13815
Vanderbilt University
10.
Cumberbatch, Derrick Cameron.
Development and Characterization of Novel Bioluminescent Reporters of Cellular Activity.
Degree: PhD, Biological Sciences, 2019, Vanderbilt University
URL: http://hdl.handle.net/1803/15408
► Over the years, periodic innovations in fluorescence microscopic technologies have afforded researchers a more sophisticated understanding of the inner workings of the cell. These methods…
(more)
▼ Over the years, periodic innovations in fluorescence microscopic technologies have afforded researchers a more sophisticated understanding of the inner workings of the cell. These methods are valuable but drawbacks include phototoxicity, photobleaching, low signal-to-noise ratio, and high background intensities distracting and sometimes disrupting the phenomena being studied. To circumvent these issues, we designed proof-of-principle bioluminescent sensors and applied them to common problems scientists face while using fluorescence imaging. Bright, bioluminescence-based, ratiometric Ca2+ sensors CalfluxVTN and CalfluxCTN can track intracellular Ca2+ concentration and can be used in conjunction with optogenetic stimulation in scenarios when fluorescence excitation illumination would be problematic. Additionally, Calflux sensors can be optimally applied to high throughput screening assays where fluorescent drug-like compounds are being screened all while taking advantage of already available microscopic technologies.
Advisors/Committee Members: C David Weaver (committee member), Qi Zhang (committee member), Carl H Johnson (committee member), Douglas G McMahon (Committee Chair).
Subjects/Keywords: bioluminescence; CalfluxCTN; CalfluxVTN; HTS; calcium; imaging
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APA (6th Edition):
Cumberbatch, D. C. (2019). Development and Characterization of Novel Bioluminescent Reporters of Cellular Activity. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15408
Chicago Manual of Style (16th Edition):
Cumberbatch, Derrick Cameron. “Development and Characterization of Novel Bioluminescent Reporters of Cellular Activity.” 2019. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/15408.
MLA Handbook (7th Edition):
Cumberbatch, Derrick Cameron. “Development and Characterization of Novel Bioluminescent Reporters of Cellular Activity.” 2019. Web. 23 Jan 2021.
Vancouver:
Cumberbatch DC. Development and Characterization of Novel Bioluminescent Reporters of Cellular Activity. [Internet] [Doctoral dissertation]. Vanderbilt University; 2019. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/15408.
Council of Science Editors:
Cumberbatch DC. Development and Characterization of Novel Bioluminescent Reporters of Cellular Activity. [Doctoral Dissertation]. Vanderbilt University; 2019. Available from: http://hdl.handle.net/1803/15408
Vanderbilt University
11.
Rajaram, Kamya.
miRNA function during zebrafish retina regeneration.
Degree: PhD, Biological Sciences, 2014, Vanderbilt University
URL: http://hdl.handle.net/1803/12985
► Zebrafish spontaneously regenerate their retinas after a variety of retinal insults. Key to regeneration are resident support cells called Müller glia (MG), which respond to…
(more)
▼ Zebrafish spontaneously regenerate their retinas after a variety of retinal insults. Key to regeneration are resident support cells called Müller glia (MG), which respond to injury by reverting to a stem cell-like state and generating progenitor cells that can proliferate, migrate and differentiate into any of the lost retinal cell types. Although studies have identified and characterized a number of genes and signaling pathways that control distinct steps of the regeneration process, very little is known about how expression of these genes is regulated. Small non-coding RNAs called microRNAs (miRNAs) regulate regeneration of various tissues in lower vertebrates. However, the functions and overall requirement of miRNAs for zebrafish retina regeneration are poorly understood. Using in vivo loss-of-function studies, we demonstrate that miRNAs are required for normal initiation and progression of retina regeneration. Small RNA high throughput sequencing revealed that miRNAs expression is dynamic during regeneration, but returned to its basal expression levels once regeneration was completed. We characterize the functions of two miRNAs that were downregulated during regeneration, miR-203 and miR-216, and discover that they regulate progenitor cell proliferation and MG dedifferentiation, respectively, during adult zebrafish retina regeneration.
Advisors/Committee Members: Ronald B Emeson (committee member), Antonis Rokas (committee member), Joshua T Gamse (committee member), James G Patton (committee member), Douglas G McMahon (Committee Chair).
Subjects/Keywords: Müller glia; retina regeneration; zebrafish; microRNA
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APA (6th Edition):
Rajaram, K. (2014). miRNA function during zebrafish retina regeneration. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12985
Chicago Manual of Style (16th Edition):
Rajaram, Kamya. “miRNA function during zebrafish retina regeneration.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/12985.
MLA Handbook (7th Edition):
Rajaram, Kamya. “miRNA function during zebrafish retina regeneration.” 2014. Web. 23 Jan 2021.
Vancouver:
Rajaram K. miRNA function during zebrafish retina regeneration. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/12985.
Council of Science Editors:
Rajaram K. miRNA function during zebrafish retina regeneration. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/12985
Vanderbilt University
12.
Tackenberg, Michael Christian.
The influence of seasonal light on the circadian system.
Degree: PhD, Neuroscience, 2019, Vanderbilt University
URL: http://hdl.handle.net/1803/12157
► The biological response to changes in seasonal light has been the subject of scientific interest for nearly 100 years. Over time, the significant role of…
(more)
▼ The biological response to changes in seasonal light has been the subject of scientific interest for nearly 100 years. Over time, the significant role of the circadian pacemaker in mediating the mammalian response to seasonal light changes has become clearer. Despite knowledge of a role in transducing this light signal into a physiological response, the exact mechanism of that transduction and the specific components of the light signal that induce those changes have yet to be fully defined. In this dissertation, the distinct components of seasonal light (duration, timing, onset-offset interval) are examined for their ability to induce persistent changes in two circadian behavioral properties implicated in seasonal responses: locomotor activity duration and free-running period. We find that the onset-offset interval is critical for determining activity duration, while net phase shift direction and light interval are critical for determining period. Further, we find that a change to phase distribution underlies changes to activity duration but not to free-running period. To interrogate the cellular mechanism of these changes, the role of a particular set of circadian clock neurons, the VIPergic neurons of the SCN, in inducing proximal changes in activity duration is examined. We find that VIPergic neuron activation by optogenetics induces proximal changes in activity duration. Lastly, a set of techniques for the assessment of relevant circadian properties both in vivo and ex vivo are presented. These include a more precise and accurate period-independent method to measure phase shifts, a higher-throughput computational approach to calculating activity duration, and a strategy for quantifying phase dispersal across bioluminescent SCN cultures. The results and techniques presented in this dissertation represent an advance in our understanding of the underlying induction of seasonal responses (both in cues and in cellular mechanism) as well as a step forward in the accurate quantification of relevant properties.
Advisors/Committee Members: Carl H. Johnson (committee member), Douglas G. McMahon (committee member), Terry L. Page (committee member), Mark T. Wallace (committee member), Ronald B. Emeson (Committee Chair).
Subjects/Keywords: photoperiod; SCN; Circadian rhythms; optogenetics
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APA (6th Edition):
Tackenberg, M. C. (2019). The influence of seasonal light on the circadian system. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12157
Chicago Manual of Style (16th Edition):
Tackenberg, Michael Christian. “The influence of seasonal light on the circadian system.” 2019. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/12157.
MLA Handbook (7th Edition):
Tackenberg, Michael Christian. “The influence of seasonal light on the circadian system.” 2019. Web. 23 Jan 2021.
Vancouver:
Tackenberg MC. The influence of seasonal light on the circadian system. [Internet] [Doctoral dissertation]. Vanderbilt University; 2019. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/12157.
Council of Science Editors:
Tackenberg MC. The influence of seasonal light on the circadian system. [Doctoral Dissertation]. Vanderbilt University; 2019. Available from: http://hdl.handle.net/1803/12157
Vanderbilt University
13.
Clanton, Joshua Aaron.
Fgf Signaling Governs the Differentiation of Parapineal Neurons in Zebrafish.
Degree: PhD, Biological Sciences, 2013, Vanderbilt University
URL: http://hdl.handle.net/1803/10639
► Parapineal precursors arise from the medially located pineal complex anlage and migrate to the left side of the brain. Published data implicates Fgf8a in the…
(more)
▼ Parapineal precursors arise from the medially located pineal complex anlage and migrate to the left side of the brain. Published data implicates Fgf8a in the migration of parapineal cells away from the midline of the pineal anlage. However, the potential role for Fgf8a during the acquisition of parapineal cell fate was not addressed. We have found that Fgf8a regulates a fate decision among specified parapineal precursors that occurs just prior to the initiation of leftward migration. Attenuation of Fgf signaling results in the loss of parapineal cells and the gain of additional cone cells. Data obtained from the combined loss of Flh and Fgf8a, as well as cell fate analysis, shows that in the absence of Fgf signaling, parapineal precursors differentiate as cone photoreceptors rather than parapineal cells. Furthermore, Fgf8a acts permissively to promote parapineal fate in conjunction with the transcription factor Tbx2b, but acts by itself to either block cone photoreceptor fate or promote parapineal differentiation. This cell fate change is independent of Bmp signaling, which promotes the formation of pineal photoreceptors. Instead, parapineal cell differentiation likely requires the two transcription factors, Lhx2b and Lhx9, which are responsive to Fgf signaling and are involved in parapineal formation.
Advisors/Committee Members: James G. Patton (committee member), Jason R. Jessen (committee member), Michael K. Cooper (committee member), Joshua T. Gamse (committee member), Douglas G. McMahon (Committee Chair).
Subjects/Keywords: Left-right asymmetry; neuron; Differentiation
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APA (6th Edition):
Clanton, J. A. (2013). Fgf Signaling Governs the Differentiation of Parapineal Neurons in Zebrafish. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10639
Chicago Manual of Style (16th Edition):
Clanton, Joshua Aaron. “Fgf Signaling Governs the Differentiation of Parapineal Neurons in Zebrafish.” 2013. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/10639.
MLA Handbook (7th Edition):
Clanton, Joshua Aaron. “Fgf Signaling Governs the Differentiation of Parapineal Neurons in Zebrafish.” 2013. Web. 23 Jan 2021.
Vancouver:
Clanton JA. Fgf Signaling Governs the Differentiation of Parapineal Neurons in Zebrafish. [Internet] [Doctoral dissertation]. Vanderbilt University; 2013. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/10639.
Council of Science Editors:
Clanton JA. Fgf Signaling Governs the Differentiation of Parapineal Neurons in Zebrafish. [Doctoral Dissertation]. Vanderbilt University; 2013. Available from: http://hdl.handle.net/1803/10639
Vanderbilt University
14.
Jones, Patrick Leo.
Functional Characterization of Odorant Receptors in Disease Vector Mosquitoes.
Degree: PhD, Biological Sciences, 2013, Vanderbilt University
URL: http://hdl.handle.net/1803/11599
► This dissertation is concerned with the expression patterns, functionality and mechanisms of disease vector mosquito odorant receptors. The principal afrotropical vector for malaria, Anopheles gambiae,…
(more)
▼ This dissertation is concerned with the expression patterns, functionality and mechanisms of disease vector mosquito odorant receptors. The principal afrotropical vector for malaria, Anopheles gambiae, discriminates human host cues through the use of a highly divergent family of odorant receptors (AgORs). In vivo, ORs form a functional complex consisting of a conserved and broadly expressed non-conventional OR co-receptor (ORco) and a conventional odor-binding OR, which provides coding specificity to each complex. I have used next-generation RNA sequencing to discriminate OR expression patterns, and identified novel candidate genes involved in chemosensory signal transduction. In addition, I have functionally characterized and identified conserved ORs between multiple disease vector mosquitoes. In an effort to identify novel modulators of AgORs for insect control, we have performed high-throughput, cell-based calcium imaging screens, and as a result, I have identified the first ORco family agonist. Through the heterologous expression of AgORco, we demonstrated the novel activity of this agonist, its effects upon the signaling properties of ORco, and elucidated a molecular mechanism of OR signaling. In addition, single unit electrophysiology studies demonstrate the ability of this ligand to activate AgORco-expressing cells in vivo. As such, ligand-mediated modulation serves as a proof-of-concept demonstration that ORco is a viable target for the development of behaviorally disruptive olfactory compounds (BDOCs) that could foster malaria reduction programs. These studies have established a new model for OR-signaling and identified a new class of molecules with the future potential to reduce global burdens of malaria and other vector borne diseases.
Advisors/Committee Members: Laurence J. Zwiebel (committee member), Kendal S. Broadie (committee member), Aurelio Galli (committee member), Brandt F. Eichman (committee member), Douglas G McMahon (Committee Chair).
Subjects/Keywords: Neuroscience; Vector Biology
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APA (6th Edition):
Jones, P. L. (2013). Functional Characterization of Odorant Receptors in Disease Vector Mosquitoes. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/11599
Chicago Manual of Style (16th Edition):
Jones, Patrick Leo. “Functional Characterization of Odorant Receptors in Disease Vector Mosquitoes.” 2013. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/11599.
MLA Handbook (7th Edition):
Jones, Patrick Leo. “Functional Characterization of Odorant Receptors in Disease Vector Mosquitoes.” 2013. Web. 23 Jan 2021.
Vancouver:
Jones PL. Functional Characterization of Odorant Receptors in Disease Vector Mosquitoes. [Internet] [Doctoral dissertation]. Vanderbilt University; 2013. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/11599.
Council of Science Editors:
Jones PL. Functional Characterization of Odorant Receptors in Disease Vector Mosquitoes. [Doctoral Dissertation]. Vanderbilt University; 2013. Available from: http://hdl.handle.net/1803/11599
Vanderbilt University
15.
Suarez, Sandra.
An Investigation of the GAPDH/Siah1 Pathway in
Human Retinal Pericyte Apoptosis.
Degree: PhD, Cell and Developmental Biology, 2015, Vanderbilt University
URL: http://hdl.handle.net/1803/15158
► Diabetic Retinopathy (DR) is a leading cause of blindness worldwide, and its prevalence is growing. Current therapies for DR address only the later stages of…
(more)
▼ Diabetic Retinopathy (DR) is a leading cause of blindness worldwide, and its prevalence is growing. Current therapies for DR address only the later stages of the disease, are invasive and are of limited effectiveness. Retinal pericyte death is an early pathologic
feature of DR. Though it has been observed in diabetic patients and in animal models of DR, the cause of pericyte death remains unknown. A novel pro-apoptotic pathway initiated by the interaction between glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the E3 ubiquitin ligase, seven in absentia homolog 1 (Siah1), was identified to play a significant role in human retinal pericyte apoptosis. Inhibition of the GAPDH/Siah1 pro-apoptotic complex blocks diabetes-induced pericyte apoptosis, widely considered a hallmark feature of DR.
Advisors/Committee Members: John S. Penn (committee member), Douglas G. McMahon (committee member), David M. Miller (committee member), Sandra S. Zinkel (committee member), John J. Reese (Committee Chair).
Subjects/Keywords: Diabetic retinopathy; cell death; apoptosis; high glucose; GAPDH; Siah1; cell signaling
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APA (6th Edition):
Suarez, S. (2015). An Investigation of the GAPDH/Siah1 Pathway in
Human Retinal Pericyte Apoptosis. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15158
Chicago Manual of Style (16th Edition):
Suarez, Sandra. “An Investigation of the GAPDH/Siah1 Pathway in
Human Retinal Pericyte Apoptosis.” 2015. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/15158.
MLA Handbook (7th Edition):
Suarez, Sandra. “An Investigation of the GAPDH/Siah1 Pathway in
Human Retinal Pericyte Apoptosis.” 2015. Web. 23 Jan 2021.
Vancouver:
Suarez S. An Investigation of the GAPDH/Siah1 Pathway in
Human Retinal Pericyte Apoptosis. [Internet] [Doctoral dissertation]. Vanderbilt University; 2015. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/15158.
Council of Science Editors:
Suarez S. An Investigation of the GAPDH/Siah1 Pathway in
Human Retinal Pericyte Apoptosis. [Doctoral Dissertation]. Vanderbilt University; 2015. Available from: http://hdl.handle.net/1803/15158
Vanderbilt University
16.
Meng, Shi.
Characterization of genetically labeled dopamine neurons and circadian studies of the zebrafish retina.
Degree: MS, Biological Sciences, 2008, Vanderbilt University
URL: http://hdl.handle.net/1803/10750
► Dopamine plays key roles in many basic functions in the central nervous system. In order to study developmental and functional roles of dopaminergic cells in…
(more)
▼ Dopamine plays key roles in many basic functions in the central nervous system. In order to study developmental and functional roles of dopaminergic cells in the zebrafish, we have examined a transgenic line of zebrafish expressing green fluorescent protein (GFP) under the control of the tyrosine hydroxylase (TH) promoter. TH-driven GFP was expressed in cells located in the inner nuclear layer. Immunocytochemistry with antibodies for GFP and TH showed that 29 ± 2% of GFP-labeled cells also expressed TH. Loose-patch voltage-clamp recording from GFPlabeled cells revealed that these dopaminergic neurons are spontaneously active in darkness. This transgenic line provides a useful tool to target retinal dopaminergic cells in vivo and in situ.
The vertebrate retina is profoundly influenced by circadian rhythmicity, yet little is known about the mechanisms of the zebrafish retinal circadian clock. To further the study of the zebrafish retinal clock, we have constructed a recombinant BAC in which short-half life GFP is under the control of the zebrafish circadian gene Per3 promoter. Expression of the modified BAC clone was observed in injected zebrafish embryos. Further intercrossing of injected fish and screening of their progeny may identify transgenic Per3::d2GFP fish. Meanwhile, by using transgenic Per3::LUC fish (generated by
G. Cahill,
University of Houston), we are able to examine the effect of light stimuli on bioluminescence rhythm at different times of a circadian cycle. Our results demonstrate that cultured zebrafish retina shows large phase shifts with phaseresponse curve close to type 0.
Advisors/Committee Members: Bruce H. Appel (committee member), Douglas G. McMahon (committee member).
Subjects/Keywords: Dopaminergic neurons; Zebra danio – Nervous system; Retina – Physiology; dopamine; circadian; zebrafish; Circadian rhythms
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APA (6th Edition):
Meng, S. (2008). Characterization of genetically labeled dopamine neurons and circadian studies of the zebrafish retina. (Thesis). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10750
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Meng, Shi. “Characterization of genetically labeled dopamine neurons and circadian studies of the zebrafish retina.” 2008. Thesis, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/10750.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Meng, Shi. “Characterization of genetically labeled dopamine neurons and circadian studies of the zebrafish retina.” 2008. Web. 23 Jan 2021.
Vancouver:
Meng S. Characterization of genetically labeled dopamine neurons and circadian studies of the zebrafish retina. [Internet] [Thesis]. Vanderbilt University; 2008. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/10750.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Meng S. Characterization of genetically labeled dopamine neurons and circadian studies of the zebrafish retina. [Thesis]. Vanderbilt University; 2008. Available from: http://hdl.handle.net/1803/10750
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Vanderbilt University
17.
Robertson, James Brian.
Shedding Light on the Yeast Respiratory Oscillation: Using Luciferase and Visible Light to Investigate Biological Rhythms in Yeast.
Degree: PhD, Biological Sciences, 2009, Vanderbilt University
URL: http://hdl.handle.net/1803/14049
► The yeast respiratory oscillation is a 3 to 5 hour biological rhythm in some strains of Saccharomyces cerevisiae that occurs under a specific range of…
(more)
▼ The yeast respiratory oscillation is a 3 to 5 hour biological rhythm in some strains of Saccharomyces cerevisiae that occurs under a specific range of growth conditions during continuous culture. The cell division cycle, in addition to transcription of many genes, oscillates along with the yeast respiratory oscillation. In this work luciferase reporters were constructed for yeast that provided automated real-time luminescent evidence of cell division synchrony and rhythmic transcriptional regulation in vivo during the yeast respiratory oscillation. This non-invasive, non-destructive luminescent system for monitoring gene activity was used to show an interrelationship between the yeast respiratory oscillation and the cell division cycle. This work also showed that visible light at an intensity of less than one tenth that of full sunlight (primarily in the blue and green wavelengths) noticeably affected the amplitude and period of the yeast respiratory oscillation by interfering with photosensitive substances required for respiration.
This dissertation constitutes a series of steps within a larger quest to fully understand the nature of biological rhythms in yeast. In addition to investigating the relationship between respiratory oscillations and cell division, other questions that motivated this research were, “Is the respiratory oscillation evidence of an endogenous biological clock?” and “Does this yeast biological rhythm shown in continuous culture exist in nature?” To pursue answers to these and related questions, a number of techniques and investigations involving the production and perception of light were used. This dissertation revolves around the use of light for exploring the biology of yeast; from developing bioluminescent yeast that report gene activity, to studying effects of visible light on yeast respiration and growth, to developing a low cost fluorescent excitation light source for use in microscopy.
Advisors/Committee Members: Kathy Gould (committee member), Antonis Rokas (committee member), Katherine L. Friedman (committee member), Carl H. Johnson (committee member), Douglas G. McMahon (Committee Chair).
Subjects/Keywords: flashlight; LED; cell division cycle; biological rhythms; circadian rhythms; luciferase; light; luminescence; oscillation; respiration; Saccharomyces cerevisiae; yeast
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APA (6th Edition):
Robertson, J. B. (2009). Shedding Light on the Yeast Respiratory Oscillation: Using Luciferase and Visible Light to Investigate Biological Rhythms in Yeast. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14049
Chicago Manual of Style (16th Edition):
Robertson, James Brian. “Shedding Light on the Yeast Respiratory Oscillation: Using Luciferase and Visible Light to Investigate Biological Rhythms in Yeast.” 2009. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/14049.
MLA Handbook (7th Edition):
Robertson, James Brian. “Shedding Light on the Yeast Respiratory Oscillation: Using Luciferase and Visible Light to Investigate Biological Rhythms in Yeast.” 2009. Web. 23 Jan 2021.
Vancouver:
Robertson JB. Shedding Light on the Yeast Respiratory Oscillation: Using Luciferase and Visible Light to Investigate Biological Rhythms in Yeast. [Internet] [Doctoral dissertation]. Vanderbilt University; 2009. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/14049.
Council of Science Editors:
Robertson JB. Shedding Light on the Yeast Respiratory Oscillation: Using Luciferase and Visible Light to Investigate Biological Rhythms in Yeast. [Doctoral Dissertation]. Vanderbilt University; 2009. Available from: http://hdl.handle.net/1803/14049
Vanderbilt University
18.
Qin, Ximing.
Cyanobacterial circadian clock in vitro and in vivo.
Degree: PhD, Biological Sciences, 2010, Vanderbilt University
URL: http://hdl.handle.net/1803/15326
► Circadian rhythms are intrinsic biological rhythms that have a period close to 24 hours. Prokaryotic cyanobacteria are the simplest organisms that show robust circadian rhythms,…
(more)
▼ Circadian rhythms are intrinsic biological rhythms that have a period close to 24 hours. Prokaryotic cyanobacteria are the simplest organisms that show robust circadian rhythms, and are the only organisms that have a circadian rhythm reconstituted in vitro in test tubes in the presence of ATP. This thesis is focused on studying the mechanism of the core circadian clockwork in the cyanobacterium Synechococcus elongatus PCC 7942. In this dissertation, I explore the mechanism of the circadian rhythm in cyanobacteria with both in vitro and in vivo studies. I elucidate the essential pattern of dynamic protein- protein interactions among three identified core clock Kai proteins, and define the relationship between the traditional TTFL (transcription translation feedback loop) model and the PTO (post translational oscillator) model within this strain. In addition, a KaiC monomer exchange phenomenon is studied to further investigate the robustness of the rhythm.
Advisors/Committee Members: Brandt Eichman (committee member), Hassane Mchaourab (committee member), Carl H. Johnson (committee member), Daniel Kaplan (committee member), Douglas G. McMahon (Committee Chair).
Subjects/Keywords: Cyanobacteria; KaiC; Phosphorylation; Circadian Clock
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APA (6th Edition):
Qin, X. (2010). Cyanobacterial circadian clock in vitro and in vivo. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15326
Chicago Manual of Style (16th Edition):
Qin, Ximing. “Cyanobacterial circadian clock in vitro and in vivo.” 2010. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/15326.
MLA Handbook (7th Edition):
Qin, Ximing. “Cyanobacterial circadian clock in vitro and in vivo.” 2010. Web. 23 Jan 2021.
Vancouver:
Qin X. Cyanobacterial circadian clock in vitro and in vivo. [Internet] [Doctoral dissertation]. Vanderbilt University; 2010. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/15326.
Council of Science Editors:
Qin X. Cyanobacterial circadian clock in vitro and in vivo. [Doctoral Dissertation]. Vanderbilt University; 2010. Available from: http://hdl.handle.net/1803/15326
Vanderbilt University
19.
Lu, Tan.
Mapping of Odor and Temperature Receptors in the Malaria Vector Mosquito Anopheles gambiae.
Degree: PhD, Biological Sciences, 2008, Vanderbilt University
URL: http://hdl.handle.net/1803/13012
► Many species of mosquitoes, including the major malaria vector Anopheles gambiae, utilize olfactory and temperature cues in host-seeking behaviors that underlie their vectorial capacity. By…
(more)
▼ Many species of mosquitoes, including the major malaria vector Anopheles gambiae, utilize olfactory and temperature cues in host-seeking behaviors that underlie their vectorial capacity. By using a combination of molecular and physiological approaches, we have studied the three head appendages of female An. gambiae mosquitoes, the antenna, the maxillary palp and the proboscis, and have characterized their respective functions in olfaction or thermoreception that might impact the host-seeking behavior of An. gambiae. Specifically, we have described three types of sensilla, the labellar T2 sensilla, the palpal capitate pegs and the antennal small coeloconica sensilla, and have characterized their responses to olfactory or temperature stimulation and have identified the underlying molecular and cellular components of these sensilla. These results facilitate the understanding of how An. gambiae mosquitoes sense human hosts that might be exploited to compromise their ability to transmit malaria.
Advisors/Committee Members: Terry L. Page (committee member), Kenneth C. Catania (committee member), Daniela Drummond-Barbosa (committee member), Laurence J. Zwiebel (committee member), Douglas G. McMahon (Committee Chair).
Subjects/Keywords: Host Location; Temperature Detection; Odorant Receptor; Malaria; Mosquito; Olfaction
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APA (6th Edition):
Lu, T. (2008). Mapping of Odor and Temperature Receptors in the Malaria Vector Mosquito Anopheles gambiae. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/13012
Chicago Manual of Style (16th Edition):
Lu, Tan. “Mapping of Odor and Temperature Receptors in the Malaria Vector Mosquito Anopheles gambiae.” 2008. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/13012.
MLA Handbook (7th Edition):
Lu, Tan. “Mapping of Odor and Temperature Receptors in the Malaria Vector Mosquito Anopheles gambiae.” 2008. Web. 23 Jan 2021.
Vancouver:
Lu T. Mapping of Odor and Temperature Receptors in the Malaria Vector Mosquito Anopheles gambiae. [Internet] [Doctoral dissertation]. Vanderbilt University; 2008. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/13012.
Council of Science Editors:
Lu T. Mapping of Odor and Temperature Receptors in the Malaria Vector Mosquito Anopheles gambiae. [Doctoral Dissertation]. Vanderbilt University; 2008. Available from: http://hdl.handle.net/1803/13012
Vanderbilt University
20.
Sun, Ziyi.
Investigation of hemi-gap-junction channels in retinal horizontal cells.
Degree: PhD, Biological Sciences, 2009, Vanderbilt University
URL: http://hdl.handle.net/1803/14900
► Hemi-gap-junction (HGJ) channels composed of connexin (Cx) proteins are proposed as the key component to mediate the negative feedback pathway from horizontal cells (HCs) to…
(more)
▼ Hemi-gap-junction (HGJ) channels composed of connexin (Cx) proteins are proposed as the key component to mediate the negative feedback pathway from horizontal cells (HCs) to photoreceptors in the outer retina. The goal of the research undertaken in this dissertation was to test whether HGJ channels in retinal HCs could serve their proposed roles in the negative feedback pathway. First, the biophysical properties of HGJ channels and their modulation by the neuromodulator zinc using electrophysiological methods were characterized. Both outward and inward hemichannel currents were elicited in cultured solitary zebrafish retinal HCs. In particular, inward hemichannel currents elicited at negative potentials persisted under physiological conditions and satisfy the requirement of ephaptic communication in the feedback pathway. In addition, to uncover the molecular basis of the HGJ currents, the function and properties of various Cx proteins were studied using morpholino-based gene knockdown and a cx55.5 mutant zebrafish. The data suggest that inward hemichannel currents are solely dependent on the expression of Cx55.5, while outward hemichannel currents are dependent by both Cx55.5 and Cx52.6. In summary, this study expands our understanding in the properties and function of HGJ channels and the mechanisms underlying neuronal network adaptation in retinal circuitry.
Advisors/Committee Members: Joshua T. Gamse (committee member), David J. Calkins (committee member), Douglas G. McMahon (committee member), Laurence J. Zwiebel (committee member), Terry L. Page (Committee Chair).
Subjects/Keywords: Hemi-Gap-Junction Channel; Retina; Horizontal Cell; Patch Clamp; Morpholino; Zebrafish
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sun, Z. (2009). Investigation of hemi-gap-junction channels in retinal horizontal cells. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14900
Chicago Manual of Style (16th Edition):
Sun, Ziyi. “Investigation of hemi-gap-junction channels in retinal horizontal cells.” 2009. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/14900.
MLA Handbook (7th Edition):
Sun, Ziyi. “Investigation of hemi-gap-junction channels in retinal horizontal cells.” 2009. Web. 23 Jan 2021.
Vancouver:
Sun Z. Investigation of hemi-gap-junction channels in retinal horizontal cells. [Internet] [Doctoral dissertation]. Vanderbilt University; 2009. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/14900.
Council of Science Editors:
Sun Z. Investigation of hemi-gap-junction channels in retinal horizontal cells. [Doctoral Dissertation]. Vanderbilt University; 2009. Available from: http://hdl.handle.net/1803/14900
Vanderbilt University
21.
Ciarleglio, Christopher Michael.
Neural Circuitry, Behavioral Correlates and Genetic Organization of the Mammalian Circadian Clock.
Degree: PhD, Neuroscience, 2009, Vanderbilt University
URL: http://hdl.handle.net/1803/11931
► Mammalian circadian rhythms are orchestrated by the suprachiasmatic nuclei (SCN) of the hypothalamus. The SCN are composed of circadian clock neurons but the mechanisms by…
(more)
▼ Mammalian circadian rhythms are orchestrated by the suprachiasmatic nuclei (SCN) of the hypothalamus. The SCN are composed of circadian clock neurons but the mechanisms by which these neuronal oscillators encode circadian physiology are incompletely understood. The overall objective of this dissertation was to determine the genetic and molecular organization of the neural network within the SCN, and elucidate how the SCN interacts with the environment to produce measurable behavioral and physiological circadian rhythms. Using genetic knockouts, molecular reporters and behavioral analyses, this dissertation explores the relationship between circadian genes, the SCN neural network in which these genes are expressed, and finally the behavioral output that results from this network in mice and humans. Overall these results suggest that circadian genes and light act together to organize the mammalian SCN neural network, and thus affect behavioral and neurological phenotypes.
Advisors/Committee Members: Douglas G. McMahon, Ph.D. (committee member), Carl H. Johnson, Ph.D. (committee member), Marshall L. Summar, M.D. (committee member), Terry L. Page, Ph.D. (Committee Chair).
Subjects/Keywords: circadian; suprachiasmatic nuclei; SCN; clock; neuroscience; neurobiology; VIP; photoperiodism; seasonality; neurogenetics; genetics; epigenetics; development
Record Details
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Record Details
Similar Records
Cite
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ciarleglio, C. M. (2009). Neural Circuitry, Behavioral Correlates and Genetic Organization of the Mammalian Circadian Clock. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/11931
Chicago Manual of Style (16th Edition):
Ciarleglio, Christopher Michael. “Neural Circuitry, Behavioral Correlates and Genetic Organization of the Mammalian Circadian Clock.” 2009. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021.
http://hdl.handle.net/1803/11931.
MLA Handbook (7th Edition):
Ciarleglio, Christopher Michael. “Neural Circuitry, Behavioral Correlates and Genetic Organization of the Mammalian Circadian Clock.” 2009. Web. 23 Jan 2021.
Vancouver:
Ciarleglio CM. Neural Circuitry, Behavioral Correlates and Genetic Organization of the Mammalian Circadian Clock. [Internet] [Doctoral dissertation]. Vanderbilt University; 2009. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/1803/11931.
Council of Science Editors:
Ciarleglio CM. Neural Circuitry, Behavioral Correlates and Genetic Organization of the Mammalian Circadian Clock. [Doctoral Dissertation]. Vanderbilt University; 2009. Available from: http://hdl.handle.net/1803/11931
. 
Open Access Theses and Dissertations