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You searched for +publisher:"University of Toronto" +contributor:("Tsao, Ming S"). Showing records 1 – 3 of 3 total matches.

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University of Toronto

1. Ting Hai, Josephine Wun. Functional Analysis of Novel Prognostic Markers of Non-small Cell Lung Cancer.

Degree: PhD, 2014, University of Toronto

Non-small cell lung cancer (NSCLC) is the leading cause of cancer deaths worldwide. Global expression profiling of patient tumours has identified hundreds of genes controlling NSCLC progression and patient survival. Our laboratory previously identified a robust 15-gene prognostic signature predictive of NSCLC patient outcome. We hypothesized that these genes may contribute to malignant progression of lung cancer. This thesis probes the functions of three signature genes as a means to classify their expression changes as causal or responsive to NSCLC. In the first study, the signature gene L1CAM was found to promote both NSCLC metastasis and cell migration and invasion. This was supported by in silico analyses, which showed that L1CAM expression is consistently an independent prognostic factor in multiple large cohorts of NSCLC patients. The second study identified the signature gene TRIM14 as a putative NSCLC tumour suppressor. Loss of TRIM14 function increased in vitro proliferation and in vivo tumorigenicity of NSCLC cell lines. To exert its function, TRIM14 interacts with apoptosis-inducing factor possibly regulating its stability. The third study explored the efficacy of molecularly targeting prognostic biomarkers such as the signature gene MDM2 in primary tumour xenograft models of NSCLC. MDM2 inhibitors were found to effectively inhibit NSCLC tumour growth by activating the p53 tumour suppressor pathway and cell cycle arrest, confirming a role for MDM2 in the oncogenesis of NSCLC. Overall, these results demonstrate that prognostic gene signatures constructed using genomics approaches are a powerful tool to uncover genes having critical roles in lung cancer progression.

2017-11-16 00:00:00

Advisors/Committee Members: Tsao, Ming S, Medical Biophysics.

Subjects/Keywords: lung cancer; Non-small cell lung cancer; 0992

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ting Hai, J. W. (2014). Functional Analysis of Novel Prognostic Markers of Non-small Cell Lung Cancer. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/79814

Chicago Manual of Style (16th Edition):

Ting Hai, Josephine Wun. “Functional Analysis of Novel Prognostic Markers of Non-small Cell Lung Cancer.” 2014. Doctoral Dissertation, University of Toronto. Accessed August 18, 2018. http://hdl.handle.net/1807/79814.

MLA Handbook (7th Edition):

Ting Hai, Josephine Wun. “Functional Analysis of Novel Prognostic Markers of Non-small Cell Lung Cancer.” 2014. Web. 18 Aug 2018.

Vancouver:

Ting Hai JW. Functional Analysis of Novel Prognostic Markers of Non-small Cell Lung Cancer. [Internet] [Doctoral dissertation]. University of Toronto; 2014. [cited 2018 Aug 18]. Available from: http://hdl.handle.net/1807/79814.

Council of Science Editors:

Ting Hai JW. Functional Analysis of Novel Prognostic Markers of Non-small Cell Lung Cancer. [Doctoral Dissertation]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/79814


University of Toronto

2. Pasko, Elena. Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer.

Degree: 2015, University of Toronto

Cancer associated fibroblasts (CAFs) represent a large portion of non-small cell lung cancer (NSCLC) stroma where they mediate extracellular matrix (ECM) remodelling and its interactions with neoplastic cells. A recognized NSCLC CAF marker, fibrillar collagen-binding receptor integrin alpha11 (11), known to promote lung tumour growth and metastasis, was highly correlated with stromal lysyl oxidase-like 1 (LOXL1) in two NSCLC patient cohorts. Both genes were up-regulated in tumour stroma compared to normal lung tissue and were associated with desmoplasia status in patient tumour samples. Over-expression of 11 resulted in up-regulation of LOXL1 in three in vitro models. When cultured within three-dimensional collagen matrices, cells with over-expressed 11 caused linearization, alignment and contraction of collagen fibres. In the absence of 11 expression, over-expression of LOXL1 rescued this phenotype. LOXL1 expression was directly proportional to tissue stiffness; degree of gel contraction; and collagen fibre linearization in in vitro and xenograft tumour models.

M.Sc.

2018-03-23 00:00:00

Advisors/Committee Members: Tsao, Ming S, Laboratory Medicine and Pathobiology.

Subjects/Keywords: Cancer associated fibroblasts; Collagen; Integrin alpha 11; Lysyl oxidase-like 1; Non-small cell lung cancer; Stroma; 0571

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Pasko, E. (2015). Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/88571

Chicago Manual of Style (16th Edition):

Pasko, Elena. “Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer.” 2015. Masters Thesis, University of Toronto. Accessed August 18, 2018. http://hdl.handle.net/1807/88571.

MLA Handbook (7th Edition):

Pasko, Elena. “Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer.” 2015. Web. 18 Aug 2018.

Vancouver:

Pasko E. Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2018 Aug 18]. Available from: http://hdl.handle.net/1807/88571.

Council of Science Editors:

Pasko E. Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/88571


University of Toronto

3. Pasko, Elena. Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer.

Degree: 2015, University of Toronto

Cancer associated fibroblasts (CAFs) represent a large portion of non-small cell lung cancer (NSCLC) stroma where they mediate extracellular matrix (ECM) remodelling and its interactions with neoplastic cells. A recognized NSCLC CAF marker, fibrillar collagen-binding receptor integrin alpha11 (11), known to promote lung tumour growth and metastasis, was highly correlated with stromal lysyl oxidase-like 1 (LOXL1) in two NSCLC patient cohorts. Both genes were up-regulated in tumour stroma compared to normal lung tissue and were associated with desmoplasia status in patient tumour samples. Over-expression of 11 resulted in up-regulation of LOXL1 in three in vitro models. When cultured within three-dimensional collagen matrices, cells with over-expressed 11 caused linearization, alignment and contraction of collagen fibres. In the absence of 11 expression, over-expression of LOXL1 rescued this phenotype. LOXL1 expression was directly proportional to tissue stiffness; degree of gel contraction; and collagen fibre linearization in in vitro and xenograft tumour models.

M.Sc.

2018-03-23 00:00:00

Advisors/Committee Members: Tsao, Ming S, Laboratory Medicine and Pathobiology.

Subjects/Keywords: Cancer associated fibroblasts; Collagen; Integrin alpha 11; Lysyl oxidase-like 1; Non-small cell lung cancer; Stroma; 0571

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Pasko, E. (2015). Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/88894

Chicago Manual of Style (16th Edition):

Pasko, Elena. “Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer.” 2015. Masters Thesis, University of Toronto. Accessed August 18, 2018. http://hdl.handle.net/1807/88894.

MLA Handbook (7th Edition):

Pasko, Elena. “Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer.” 2015. Web. 18 Aug 2018.

Vancouver:

Pasko E. Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2018 Aug 18]. Available from: http://hdl.handle.net/1807/88894.

Council of Science Editors:

Pasko E. Stromal Lysyl Oxidase-Like 1 Increases Extracellular Matrix Stiffness and Enhances Collagen Fibre Alignment in Non-Small Cell Lung Cancer. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/88894

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