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You searched for +publisher:"University of Toronto" +contributor:("Goring, Daphne"). Showing records 1 – 3 of 3 total matches.

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University of Toronto

1. Johnson, Daniel Clinton. Trafficking Dynamics in Arabidopsis Pollen-stigma Interactions.

Degree: 2016, University of Toronto

Reproduction in the Brassicaceae family depends on self-recognition, where cross-compatible pollen is distinguished from self-incompatible pollen prior to acceptance by the stigma. The stigma only gives compatible pollen the resources required for hydration and germination of a pollen tube for fertilization. These resources are sequestered away from self-incompatible pollen, inhibiting inbreeding. Many questions remain surrounding the specific signalling events that govern this self-recognition system. This research investigates how resource delivery to self-incompatible pollen is prevented in Arabidopsis. Topics explored include the localization of Sec5a, an exocyst complex member that delivers secretory vesicles to compatible pollen; the localization of ARC1, an E3 ubiquitin ligase which prevents the exocyst from delivering resources; and autophagyâ s degradation of the sequestered resources.

M.Sc.

2018-12-06 00:00:00

Advisors/Committee Members: Goring, Daphne, Cell and Systems Biology.

Subjects/Keywords: Reproduction; Self-Incompatibility; Signalling; 0479

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Johnson, D. C. (2016). Trafficking Dynamics in Arabidopsis Pollen-stigma Interactions. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/92783

Chicago Manual of Style (16th Edition):

Johnson, Daniel Clinton. “Trafficking Dynamics in Arabidopsis Pollen-stigma Interactions.” 2016. Masters Thesis, University of Toronto. Accessed October 16, 2019. http://hdl.handle.net/1807/92783.

MLA Handbook (7th Edition):

Johnson, Daniel Clinton. “Trafficking Dynamics in Arabidopsis Pollen-stigma Interactions.” 2016. Web. 16 Oct 2019.

Vancouver:

Johnson DC. Trafficking Dynamics in Arabidopsis Pollen-stigma Interactions. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2019 Oct 16]. Available from: http://hdl.handle.net/1807/92783.

Council of Science Editors:

Johnson DC. Trafficking Dynamics in Arabidopsis Pollen-stigma Interactions. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/92783


University of Toronto

2. Haasen, Katrina Ellen. An Investigation of the Exocyst Complex and its role in Compatible Pollen-pistil Interactions in Arabidopsis.

Degree: 2010, University of Toronto

Compatible interactions between male gametophytes (pollen) and the female reproductive organ (pistil) are essential for fertilization in flowering plants. Recognition at a molecular level allows “compatible” pollen grains to adhere/germinate on the stigma while pollen grains from unrelated plant species are largely ignored. The exocyst is a large eight subunit complex that is primarily involved in polarized secretion or regulated exocytosis in eukaryotic cells where it functions to tether vesicles to the plasma membrane. Recent research has implicated one of the Exo70 family members, Exo70A1, in compatible pollen-pistil interactions in Arabidopsis and Brassica. The loss of Exo70A1 in Arabidopsis Col-0 stigmas leads to the rejection of compatible pollen producing a “female sterile” phenotype. Through my research I have demonstrated that, driven by a stigma-specific promoter, an RFP:Exo70A1 fusion protein rescues this defect in exo70A1-1 mutant and Exo70A1 is found to be localized to the plasma membrane at flower opening.

MAST

Advisors/Committee Members: Goring, Daphne, Cell and Systems Biology.

Subjects/Keywords: Exocyst Complex; Arabidopsis; Molecular signaling; Plant Reproduction; Pollen-Pistil Interactions; Compatibility; Self-Incompatibility; Exo70A1; Vesicle Trafficking; Docking Complexes; Brassica; Stigma; Papillae Cells; 0307; 0309

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Haasen, K. E. (2010). An Investigation of the Exocyst Complex and its role in Compatible Pollen-pistil Interactions in Arabidopsis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/24273

Chicago Manual of Style (16th Edition):

Haasen, Katrina Ellen. “An Investigation of the Exocyst Complex and its role in Compatible Pollen-pistil Interactions in Arabidopsis.” 2010. Masters Thesis, University of Toronto. Accessed October 16, 2019. http://hdl.handle.net/1807/24273.

MLA Handbook (7th Edition):

Haasen, Katrina Ellen. “An Investigation of the Exocyst Complex and its role in Compatible Pollen-pistil Interactions in Arabidopsis.” 2010. Web. 16 Oct 2019.

Vancouver:

Haasen KE. An Investigation of the Exocyst Complex and its role in Compatible Pollen-pistil Interactions in Arabidopsis. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2019 Oct 16]. Available from: http://hdl.handle.net/1807/24273.

Council of Science Editors:

Haasen KE. An Investigation of the Exocyst Complex and its role in Compatible Pollen-pistil Interactions in Arabidopsis. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/24273


University of Toronto

3. Chapman, Laura. The Role of Sec15b and Phosphatidylinositol-4-Phosphate in Early Compatible Pollen-pistil Interactions.

Degree: 2010, University of Toronto

Stigmas of Arabidopsis thaliana carrying the exo70A1-1 mutation are defective in early pollen-pistil interactions; pollen grain adhesion to the stigma, pollen hydration, and penetration of the stigmatic surface by the pollen tube. Exo70 function in directed secretion has been linked to its ability to bind the phosphatidylinositides. To provide support that the classical, octomeric exocyst complex, which contains the Exo70 subunit, participates in compatible pollen-pistil interactions, this process was analyzed in plants deficient in Sec15, another subunit of the exocyst. Additionally, the role of phosphatidylinositol-4-phosphate (PI-4-P) in female fertility was evaluated through the use of the mutants ROOT HAIR DEFECTIVE 4-1 and PI4Kβ1β2 -/-, which have increased and decreased levels of PI-4-P respectively. Reduction of Sec15b levels or perturbation of PI-4-P in the stigma resulted in a reduced ability of of the transgenic/mutant stigmas to support pollen grain hydration; though all other stages of early pollen pistil interactions were unaffected.

MAST

Advisors/Committee Members: Goring, Daphne, Cell and Systems Biology.

Subjects/Keywords: exocyst; pollination; 0379; 0309

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Chapman, L. (2010). The Role of Sec15b and Phosphatidylinositol-4-Phosphate in Early Compatible Pollen-pistil Interactions. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/25450

Chicago Manual of Style (16th Edition):

Chapman, Laura. “The Role of Sec15b and Phosphatidylinositol-4-Phosphate in Early Compatible Pollen-pistil Interactions.” 2010. Masters Thesis, University of Toronto. Accessed October 16, 2019. http://hdl.handle.net/1807/25450.

MLA Handbook (7th Edition):

Chapman, Laura. “The Role of Sec15b and Phosphatidylinositol-4-Phosphate in Early Compatible Pollen-pistil Interactions.” 2010. Web. 16 Oct 2019.

Vancouver:

Chapman L. The Role of Sec15b and Phosphatidylinositol-4-Phosphate in Early Compatible Pollen-pistil Interactions. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2019 Oct 16]. Available from: http://hdl.handle.net/1807/25450.

Council of Science Editors:

Chapman L. The Role of Sec15b and Phosphatidylinositol-4-Phosphate in Early Compatible Pollen-pistil Interactions. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/25450

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