Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for +publisher:"University of Tennessee – Knoxville" +contributor:("Timothy E. Sparer, Alison Buchan"). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of Tennessee – Knoxville

1. Mead, Thomas James. An Analytical Survey of a Biotinylated Bacteriophage System for Quantifying Transduction Events in Natural Ecosystems.

Degree: MS, Microbiology, 2012, University of Tennessee – Knoxville

Bacteriophage transduction events play a contributive role in bacterial gene diffusion in ecosystems comprised of natural microbial populations. The rate at which these phage-mediated genetic transfers occur in wastewater ecosystems is not well understood. This work describes the effectiveness of a genetically engineered biotinylated bacteriophage T4 system as a tool for examining transduction event rates and compositions in environments that pose increased risk of antibiotic resistance proliferation. Antibiotic use has steadily increased over the past century, giving rise to antibiotic resistance in microorganisms. Agricultural, medical, and industrial use of antibiotics produces waste teeming with residual antibiotic compounds that are collected to form novel microbial ecosystems. These wastewater microbial communities include, among others, bacteria and their viral parasites, bacteriophage. Bacteriophage can transfer genetic material between bacterial hosts through a phenomenon known as transduction, thus creating circumstances that can contribute to the spread of antibiotic resistance genes. Wastewater ecosystems that contain vestigial antibiotic compounds may produce selective breeding grounds for the development of antibiotic resistant microorganisms. This study aimed to elucidate the role of bacteriophage in the spread of antibiotic resistant genes under these environmental conditions using a novel biotin-based tagging and recovery system. This system analyzed the unbiased tagging of bacteriophage particles, infection of host bacteria, and the recovery of biotinylated phage progeny as means to investigate phage-mediated transduction rates. A recombinant bacteriophage T4 with biotin carboxylase carrier protein (BCCP) fused to the small outer capsid (SOC) acted as the tagged bacteriophage in this work. Numerous techniques for phage propagation and purification were optimized for this application, indicating important information about the feasibility of using this engineered bacteriophage in a natural ecosystem. The genetic instability and susceptibility to selective pressures of the biotinylated bacteriophage, even under optimally controlled conditions, resulted in the deactivation of the phage’s biotinylation capabilities. The results of this examination indicate the impracticality of using this genetic-based biotinylation approach as a method for measuring transduction events rates in natural ecosystems. Advisors/Committee Members: Gary S. Sayler, Timothy E. Sparer, Alison Buchan.

Subjects/Keywords: Microbiology

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mead, T. J. (2012). An Analytical Survey of a Biotinylated Bacteriophage System for Quantifying Transduction Events in Natural Ecosystems. (Thesis). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_gradthes/1182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Mead, Thomas James. “An Analytical Survey of a Biotinylated Bacteriophage System for Quantifying Transduction Events in Natural Ecosystems.” 2012. Thesis, University of Tennessee – Knoxville. Accessed February 21, 2019. https://trace.tennessee.edu/utk_gradthes/1182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Mead, Thomas James. “An Analytical Survey of a Biotinylated Bacteriophage System for Quantifying Transduction Events in Natural Ecosystems.” 2012. Web. 21 Feb 2019.

Vancouver:

Mead TJ. An Analytical Survey of a Biotinylated Bacteriophage System for Quantifying Transduction Events in Natural Ecosystems. [Internet] [Thesis]. University of Tennessee – Knoxville; 2012. [cited 2019 Feb 21]. Available from: https://trace.tennessee.edu/utk_gradthes/1182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mead TJ. An Analytical Survey of a Biotinylated Bacteriophage System for Quantifying Transduction Events in Natural Ecosystems. [Thesis]. University of Tennessee – Knoxville; 2012. Available from: https://trace.tennessee.edu/utk_gradthes/1182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.