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You searched for +publisher:"University of Tennessee – Knoxville" +contributor:("Jun Lin, Gina M. Pighetti"). Showing records 1 – 2 of 2 total matches.

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University of Tennessee – Knoxville

1. Merrill, Caitlin Elizabeth. Immunological Responses and Protection in Dairy Cows Vaccinated with <i>Staphylococcus aureus</i> Surface Proteins (SASP) and <i>Staphylococcus chromogenes</i> Surface Proteins (SCSP).

Degree: MS, Animal Science, 2018, University of Tennessee – Knoxville

Bovine mastitis is the major cause of economic losses in dairy production worldwide. <i>Staphylococcus aureus</i> is a major causative agent that possesses multiple virulence factors responsible for successful colonization of mammary glands. Despite the adoption of current mastitis control measures, <i>S. aureus</i> continues to be one of the most prevalent mastitis pathogens throughout the world. Lysigin® (Boehringer Ingelheim Vetmedica, Inc, St. Joseph, MO) is a commercial <i>S. aureus</i> vaccine currently available in the US and Startvac® (Hipra, Girona, Spain) is a commercial <i>S. aureus</i> vaccine in Europe. Although some studies evaluated efficacy of these vaccines reported reduction in the duration and intensity of clinical signs, none of them prevent <i>S. aureus</i> intramammary infection (IMI) in either field trials or under controlled experimental studies. Because of the tendency of this organism to cause chronic IMI, treatment with antibiotics is of limited success. Therefore, there has been an increasing demand for alternative control measures, such as a vaccine to effectively prevent <i>S. aureus</i> IMI. The objectives of this study were to evaluate the immune responses and protection against <i>S. aureus</i> IMI in dairy cows vaccinated with <i>Staphylococcus aureus</i> surface proteins (SASP) and <i>Staphylococcus chromogenes</i> surface proteins (SCSP). A total of 18 pregnant Holstein dairy cows ranging from heifers to 3rd lactation cows were divided into three groups of 6 animals each. Animals in Groups 1 and 2 were vaccinated with 1.2 mg/dose of SASP and SCSP proteins with Emulsigen-D adjuvant, respectively. Animals in Group 3 were injected with PBS mixed with Emulsigen-D at equal proportion (1. 5 ml each) and used as control. Animals were vaccinated subcutaneously in the neck area during late lactation at 28 (D-28) and 14 (D-14) days before drying off, and at drying off (D0). Subsequently, each animal was challenged with <i>S. aureus</i> strain 60 by teat dipping in bacterial suspension at 107 CFU/ml culture medium. Results showed that vaccinated cows had increased milk and serum antibody titers and reduced bacterial shedding through milk. Interestingly, SCSP vaccine cross-protected against <i>S. aureus</i> clinical mastitis thus suggesting its potential as immunogenic antigens to control bovine <i>S. aureus</i> mastitis. Advisors/Committee Members: Oudessa Kerro Dego, Jun Lin, Gina M. Pighetti.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Merrill, C. E. (2018). Immunological Responses and Protection in Dairy Cows Vaccinated with <i>Staphylococcus aureus</i> Surface Proteins (SASP) and <i>Staphylococcus chromogenes</i> Surface Proteins (SCSP). (Thesis). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_gradthes/5125

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Merrill, Caitlin Elizabeth. “Immunological Responses and Protection in Dairy Cows Vaccinated with <i>Staphylococcus aureus</i> Surface Proteins (SASP) and <i>Staphylococcus chromogenes</i> Surface Proteins (SCSP).” 2018. Thesis, University of Tennessee – Knoxville. Accessed February 29, 2020. https://trace.tennessee.edu/utk_gradthes/5125.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Merrill, Caitlin Elizabeth. “Immunological Responses and Protection in Dairy Cows Vaccinated with <i>Staphylococcus aureus</i> Surface Proteins (SASP) and <i>Staphylococcus chromogenes</i> Surface Proteins (SCSP).” 2018. Web. 29 Feb 2020.

Vancouver:

Merrill CE. Immunological Responses and Protection in Dairy Cows Vaccinated with <i>Staphylococcus aureus</i> Surface Proteins (SASP) and <i>Staphylococcus chromogenes</i> Surface Proteins (SCSP). [Internet] [Thesis]. University of Tennessee – Knoxville; 2018. [cited 2020 Feb 29]. Available from: https://trace.tennessee.edu/utk_gradthes/5125.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Merrill CE. Immunological Responses and Protection in Dairy Cows Vaccinated with <i>Staphylococcus aureus</i> Surface Proteins (SASP) and <i>Staphylococcus chromogenes</i> Surface Proteins (SCSP). [Thesis]. University of Tennessee – Knoxville; 2018. Available from: https://trace.tennessee.edu/utk_gradthes/5125

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of Tennessee – Knoxville

2. Vaughn, Jacqueline M. Virulence Characteristics and Genetic Diversity of <i>Staphylococcus aureus</i> Isolates from Cases of Bovine Mastitis in Eastern Tennessee.

Degree: MS, Animal Science, 2018, University of Tennessee – Knoxville

<i>Staphylococcus aureus</i> is an important zoonotic mastitis pathogen that has significant effects on animal and human health. <i>S. aureus</i> is also a foodborne pathogen that causes food poisoning through its diverse enterotoxins. Some studies showed that <i>S. aureus</i> strains that cause infection in a particular host are genetically distinct and are host specific, although most strains are believed to be infective to a wide range of host species. However, there are no clearly defined clonal patterns of <i>S. aureus</i> that possess certain virulence factors responsible for causing a disease. The objectives of this study were: 1) evaluate clonal diversity of <i>S. aureus</i> isolates from cases of bovine mastitis 2) determine staphylococcal enterotoxin production patterns 3) evaluate <i>in vitro</i> adhesion and invasion ability of dominant strains on bovine mammary epithelial cell line (MAC-T cells). Milk samples from bovine mastitis were evaluated at Tennessee Quality Milk Laboratory (TQML) for causative agents. Overall, 111 <i>S. aureus</i> strains were isolated and evaluated for genetic diversity by pulsed field gel electrophoresis (PFGE) and for presence of toxin genes that encode for staphylococcal enterotoxins (<i>sea</i>, <i>seb</i>, <i>sec</i>, <i>see</i>, <i>sej</i>) and toxic shock syndrome toxin 1 (<i>tsst-1</i>) by PCR. The <i>in vitro</i> adhesion and invasion ability of the dominant strains were evaluated on mammary epithelial cell line (MAC-T). We found 16 PFGE types (dominant clones) ranging from A – P. The PFGE type M is the most prevalent of all 16 PFGE types. The PCR results of enterotoxins genes showed that some of these strains were positive for staphylococcal enterotoxin genes including <i>seb</i> (11.7%), <i>sec</i> (2.7%), <i>see</i> (0.9%) and toxic shock syndrome toxin 1 (<i>tsst-1</i>) (7.2%), whereas most strains (75.7%) were negative for enterotoxin genes. In addition, evaluation of association of PFGE types, enterotoxins and other virulence factors, that were evaluated previously, showed that PFGE types O and M tend to cluster with beta-hemolysin, absence of enterotoxins and susceptibility to antimicrobials. Analysis of <i>in vitro</i> adhesion to and invasion into MAC-T cells showed relatively higher number of O strain adhered to and invaded into MAC-T cells followed by M and I strains however were not statistically significant. Advisors/Committee Members: Oudessa Kerro Dego, Jun Lin, Gina M. Pighetti.

Subjects/Keywords: Dairy; Enterotoxins; PFGE; Bovine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Vaughn, J. M. (2018). Virulence Characteristics and Genetic Diversity of <i>Staphylococcus aureus</i> Isolates from Cases of Bovine Mastitis in Eastern Tennessee. (Thesis). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_gradthes/5185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Vaughn, Jacqueline M. “Virulence Characteristics and Genetic Diversity of <i>Staphylococcus aureus</i> Isolates from Cases of Bovine Mastitis in Eastern Tennessee.” 2018. Thesis, University of Tennessee – Knoxville. Accessed February 29, 2020. https://trace.tennessee.edu/utk_gradthes/5185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Vaughn, Jacqueline M. “Virulence Characteristics and Genetic Diversity of <i>Staphylococcus aureus</i> Isolates from Cases of Bovine Mastitis in Eastern Tennessee.” 2018. Web. 29 Feb 2020.

Vancouver:

Vaughn JM. Virulence Characteristics and Genetic Diversity of <i>Staphylococcus aureus</i> Isolates from Cases of Bovine Mastitis in Eastern Tennessee. [Internet] [Thesis]. University of Tennessee – Knoxville; 2018. [cited 2020 Feb 29]. Available from: https://trace.tennessee.edu/utk_gradthes/5185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vaughn JM. Virulence Characteristics and Genetic Diversity of <i>Staphylococcus aureus</i> Isolates from Cases of Bovine Mastitis in Eastern Tennessee. [Thesis]. University of Tennessee – Knoxville; 2018. Available from: https://trace.tennessee.edu/utk_gradthes/5185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.