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You searched for +publisher:"University of Southern Mississippi" +contributor:("Janis O\'Donnell"). Showing records 1 – 2 of 2 total matches.

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University of Southern Mississippi

1. Chen, Qichuan. The Drosophila T-box Transcription Factor Midline Functions within the Insulin/AKT and c-Jun-N-terminal Kinase Signaling Pathways to Regulate Interomatidial Bristle Formation and Cell Survival.

Degree: MS, Biological Sciences, 2014, University of Southern Mississippi

From a genetic and allelic modifier screen, we report that the Drosophila melanogaster T-box transcription factor midline (mid), a homolog to the human TBX20 gene, interacts with dFOXO within the insulin receptor (InR) and the c-Jun-N-terminal kinase (JNK) signaling pathways to regulate interommatidial bristle (IOB) formation. Previous studies have identified mid’s role in cell fate specification of sensory organ precursor cells in conjunction with the Notch-Delta signaling pathway (Das et al., 2013). The Notch, InR, and JNK signaling pathways regulate dFOXO activity under conditions of stress. Thus, we determined the effects of oxidative stress and metabolic stress by exposing mid-RNAi flies to paraquat and starvation conditions, respectively. We found that oxidative stress suppressed the mid-RNAi phenotype while starvation had no significant effect. We next assayed Mid and H15, a paralog of Mid, via Western blot analysis and report that Mid exhibits a nucleocytoplasmic distribution pattern that is altered within the mid-RNAi mutant while H15 was found exclusively within the cytoplasmic fraction. This opens the possibility that Mid and/or H15 may regulate cytoplasmic targets upstream of dFOXO. The evidence suggests that Mid utilizes the InR, JNK, and Notch signaling pathways to regulate cell fate specification, differentiation, and survival during third instar larval development. Advisors/Committee Members: Sandra Leal, Hao Xu, Janis O'Donnell.

Subjects/Keywords: mid; midline; Tbx20; dFOXO; C-Jun-N-terminal kinase; insulin receptor; Developmental Biology

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APA (6th Edition):

Chen, Q. (2014). The Drosophila T-box Transcription Factor Midline Functions within the Insulin/AKT and c-Jun-N-terminal Kinase Signaling Pathways to Regulate Interomatidial Bristle Formation and Cell Survival. (Masters Thesis). University of Southern Mississippi. Retrieved from https://aquila.usm.edu/masters_theses/76

Chicago Manual of Style (16th Edition):

Chen, Qichuan. “The Drosophila T-box Transcription Factor Midline Functions within the Insulin/AKT and c-Jun-N-terminal Kinase Signaling Pathways to Regulate Interomatidial Bristle Formation and Cell Survival.” 2014. Masters Thesis, University of Southern Mississippi. Accessed April 22, 2019. https://aquila.usm.edu/masters_theses/76.

MLA Handbook (7th Edition):

Chen, Qichuan. “The Drosophila T-box Transcription Factor Midline Functions within the Insulin/AKT and c-Jun-N-terminal Kinase Signaling Pathways to Regulate Interomatidial Bristle Formation and Cell Survival.” 2014. Web. 22 Apr 2019.

Vancouver:

Chen Q. The Drosophila T-box Transcription Factor Midline Functions within the Insulin/AKT and c-Jun-N-terminal Kinase Signaling Pathways to Regulate Interomatidial Bristle Formation and Cell Survival. [Internet] [Masters thesis]. University of Southern Mississippi; 2014. [cited 2019 Apr 22]. Available from: https://aquila.usm.edu/masters_theses/76.

Council of Science Editors:

Chen Q. The Drosophila T-box Transcription Factor Midline Functions within the Insulin/AKT and c-Jun-N-terminal Kinase Signaling Pathways to Regulate Interomatidial Bristle Formation and Cell Survival. [Masters Thesis]. University of Southern Mississippi; 2014. Available from: https://aquila.usm.edu/masters_theses/76


University of Southern Mississippi

2. Das, Sudeshna. The Identification of the Mid Transcription Factor Regulatory Network Specifying Cell Fate and Regulating Survival in the Drosophila Eye.

Degree: MS, Biological Sciences, 2013, University of Southern Mississippi

The Drosophila melanogaster T-box transcription factor Midline (Mid) exhibits a high degree of structural and functional conservation with its vertebrate homolog Tbx-20. Both Mid and Tbx-20 regulate cell-fate specification with in diverse tissues including the central nervous system (CNS) and heart. Although, some important studies reported that Tbx-20 transcripts express in a wide range of developing mammalian eye tissues including those of the human fetus, the function of this transcription factor is unknown in the development of eye tissue. This current study is the first attempt to show that Mid and its para log H15 are expressed within distinct ommatidial cell types including photoreceptor neurons and sensory organ precursor (SOP) cells during early stages of pupal eye imaginal disc morphogensis and also identities a Mid transcription factor network regulating eye development. Reducing the expression of mid transcripts within eye disc tissues using RNA interference (mid-RNAi) results in the loss of interommatidial bristles (JOBs) in the adult eye due to the misspecification of sensory organ precursor (SOP) cells and increased levels of apoptosis induced during earlier stages of pupal development. Since the Notch-Delta signaling pathway specifies the SOP cell fate, we sought to place mid within the Notch-Delta genetic hierarchy specifying SOP cell fates. We determined that Mid functions downstream of the Notch receptor and upstream of the Enhancer of Split gene complex [E(Spl)]. We also discovered mid collaborates with two Notch pathway members also implicated in the regulation of cell survival, extramacrochaetae (emc) and senseless (sens). Moreover, toward identifying the Mid regulatory transcription factor network specifying cell fate, we found that mid collaborates with dFOXO. The dFOXO transcription factor is distinct in that it regulates cell proliferation and homeostasis within Insulin regulated stress induced pathway. The culmination of these studies suggests that Mid regulates cell fate specification in collaboration with Notch-Delta signaling pathway and also play important role in cell survival pathways essential for maintaining homeostasis. Advisors/Committee Members: Sandra Leal, Glenmore Shearer, Janis O'Donnell.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Das, S. (2013). The Identification of the Mid Transcription Factor Regulatory Network Specifying Cell Fate and Regulating Survival in the Drosophila Eye. (Masters Thesis). University of Southern Mississippi. Retrieved from https://aquila.usm.edu/masters_theses/453

Chicago Manual of Style (16th Edition):

Das, Sudeshna. “The Identification of the Mid Transcription Factor Regulatory Network Specifying Cell Fate and Regulating Survival in the Drosophila Eye.” 2013. Masters Thesis, University of Southern Mississippi. Accessed April 22, 2019. https://aquila.usm.edu/masters_theses/453.

MLA Handbook (7th Edition):

Das, Sudeshna. “The Identification of the Mid Transcription Factor Regulatory Network Specifying Cell Fate and Regulating Survival in the Drosophila Eye.” 2013. Web. 22 Apr 2019.

Vancouver:

Das S. The Identification of the Mid Transcription Factor Regulatory Network Specifying Cell Fate and Regulating Survival in the Drosophila Eye. [Internet] [Masters thesis]. University of Southern Mississippi; 2013. [cited 2019 Apr 22]. Available from: https://aquila.usm.edu/masters_theses/453.

Council of Science Editors:

Das S. The Identification of the Mid Transcription Factor Regulatory Network Specifying Cell Fate and Regulating Survival in the Drosophila Eye. [Masters Thesis]. University of Southern Mississippi; 2013. Available from: https://aquila.usm.edu/masters_theses/453

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