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You searched for +publisher:"University of South Africa" +contributor:("Eloff, J. N. (Jacobus Nicolaas)"). One record found.

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University of South Africa

1. Kayoka, Prudence Ngalula. Characterization of mycobacteria SPP. and antimycobacterial activities of plant derived compounds from Anacardiaceae family.

Degree: 2016, University of South Africa

The treatment of tuberculosis (TB) is currently a challenge due to multi- and extensively drug resistant strains of Mycobacterium tuberculosis. Mycobacterium bovis and M. tuberculosis cause clinically indistinguishable tuberculosis in humans. Both M. bovis and M. tuberculosis have been isolated from humans and animals. Plant species contain antimicrobial compounds that may lead to new anti-TB drugs. To conduct in vitro antimycobacterial assays, it is important to include current clinical isolates as new strains of bacteria might be circulating under the ongoing climate change environment. The overall goal and objectives of this study were to isolate and characterize mycobacteria species from South Africa, to test some selected plant species of the Anacardiaceae family for antimycobacterial activity using some of the newly isolated and reference strains of mycobacteria followed by cytotoxicity evaluation of the most active plant species, and finally the isolation and characterization of at least one compound from the most active and least toxic plant. This study led to the discovery of a new isolate of Mycobacterium Avium Complex species from black wildebeest. Other non-tuberculous mycobacteria and M. bovis isolates were identified from other animal species. Five out of 15 plant species screened showed good activity against Mycobacterium species. Five antimycobacterial compounds were isolated from Searsia undulata, the most active plant species. Two out of the five compounds were identified, and one compound appears to be novel, but both compounds have been isolated for the first time from Searsia undulata. An incidental finding was the potential anticancer property of extracts of Searsia undulata. Recommended future activities include isolation and identification of more active compounds from Searsia undulata which were visible in bioautography analysis, as well as synergy evaluation of antimycobacterial activities of the different compounds with current anti-tubercular drugs. Advisors/Committee Members: McGraw, L. J (advisor), Eloff, J. N. (Jacobus Nicolaas) (advisor), Obi, Chikwelu Larry (advisor).

Subjects/Keywords: Characterization; Antimycobacterial; NTM mycobacteria; Black wildebeest; MDR-M. bovis; MDR-M. tuberculosis; Anacardiaceae; Searsia undulata; Betulonic acid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kayoka, P. N. (2016). Characterization of mycobacteria SPP. and antimycobacterial activities of plant derived compounds from Anacardiaceae family. (Doctoral Dissertation). University of South Africa. Retrieved from http://hdl.handle.net/10500/22719

Chicago Manual of Style (16th Edition):

Kayoka, Prudence Ngalula. “Characterization of mycobacteria SPP. and antimycobacterial activities of plant derived compounds from Anacardiaceae family.” 2016. Doctoral Dissertation, University of South Africa. Accessed September 22, 2019. http://hdl.handle.net/10500/22719.

MLA Handbook (7th Edition):

Kayoka, Prudence Ngalula. “Characterization of mycobacteria SPP. and antimycobacterial activities of plant derived compounds from Anacardiaceae family.” 2016. Web. 22 Sep 2019.

Vancouver:

Kayoka PN. Characterization of mycobacteria SPP. and antimycobacterial activities of plant derived compounds from Anacardiaceae family. [Internet] [Doctoral dissertation]. University of South Africa; 2016. [cited 2019 Sep 22]. Available from: http://hdl.handle.net/10500/22719.

Council of Science Editors:

Kayoka PN. Characterization of mycobacteria SPP. and antimycobacterial activities of plant derived compounds from Anacardiaceae family. [Doctoral Dissertation]. University of South Africa; 2016. Available from: http://hdl.handle.net/10500/22719

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