+publisher:"University of Saskatchewan" +contributor:("Muir, Gillian")

University of Saskatchewan

1. al-shanoon, hayder. Effect of Testosterone on Ovarian Function in Beef Heifers.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-01-330

► Synchronization of follicular wave emergence forms the basis of many reproductive management techniques used in the cattle industry such as estrus synchronization, fixed-time artificial insemination… (more)

▼ Synchronization of follicular wave emergence forms the basis of many reproductive management techniques used in the cattle industry such as estrus synchronization, fixed-time artificial insemination and embryo transfer. Estrogen in combination with progesterone has been used widely for this purpose due to ease of use and efficient, consistent and quick results, irrespective of the status of the dominant follicle or corpus luteum when the treatment is applied. The recent ban by the European Union on use of estrogens in food-producing animals prompted us to examine if testosterone could be used as an alternative drug for follicular wave synchronization. We tested the hypothesis that the administration of testosterone will shorten the life-span of the extant dominant follicle resulting in early emergence of a new follicular wave. We tested two forms of testosterone (conjugated form: testosterone enanthate and unconjugated form: non-esterified free testosterone) and determined that intramuscular injection of oil-based preparation of the unconjugated form caused a sharp rise in plasma concentrations of testosterone followed by a less rapid decline. The pharmacokinetics of testosterone in blood plasma of 16 heifers was determined after two intramuscular injections of 200 mg of unconjugated testosterone (in 4 ml canola oil) at 12 hour intervals. Testosterone C max was 13.9 ng/mL and the distribution half-life of testosterone in the bloodstream was 3.2 days. Plasma testosterone concentrations were elevated within 2 hours, maintained for initial 36 hours and declined to baseline over 13 days. To study the ovarian and endocrine effects of testosterone, heifers (n=6 per group) were given two intramuscular injections of 200 mg of unconjugated testosterone in 4 mL canola oil at 12 hour intervals on Days 1 (T1), 3 (T3) or 6 (T6) of the first follicular wave (Day 0 = ovulation); the Control group was given 4 mL canola oil. Ovarian structures were monitored daily by transrectal ultrasonography over one interovulatory interval and plasma samples were collected. Following treatment, the dominant follicle grew more slowly for the next 5 days in T1 (P=0.05) and T3 (P=0.06) groups compared to the Control group, while the dominant follicle in the T6 group regressed more slowly (P=0.02) than in the Control group. The diameter profile of the dominant follicle of the post-treatment wave (Wave 2) and the ovulatory wave did not differ between treatment and Control groups. Overall, testosterone treatment (T1, T3 and T6 combined) extended the duration of current (Wave 1; P=<0.001) wave in 8 out of 17 heifers compared with the Control group and emergence of the post-treatment wave was not synchronized. Although the interovulatory interval was not affected by the treatments, the proportion of 2-wave cycles tended to be higher (P=0.08) after treatment (T1, T3 and T6 combined) compared to the Control group. The plasma LH concentrations in T1 and T3 groups decreased after treatment, while it did not change in T6 group compared to Control group.… Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Mapletoft, Reuben, Muir, Gillian.

Subjects/Keywords: Testosterone; follicle development; cattle; corpus luteum; ultrasonography; follicular and luteal dynamics; FSH; LH; Pharmacokinetics

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APA (6^th Edition):

al-shanoon, h. (2012). Effect of Testosterone on Ovarian Function in Beef Heifers. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-01-330

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

al-shanoon, hayder. “Effect of Testosterone on Ovarian Function in Beef Heifers.” 2012. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2012-01-330.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

al-shanoon, hayder. “Effect of Testosterone on Ovarian Function in Beef Heifers.” 2012. Web. 13 Apr 2021.

Vancouver:

al-shanoon h. Effect of Testosterone on Ovarian Function in Beef Heifers. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2012-01-330.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

al-shanoon h. Effect of Testosterone on Ovarian Function in Beef Heifers. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-01-330

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

2. Alfoteisy, Bilal. Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-01-336

► The main objective of this study was to investigate if natural honey can be used as a cryoprotecting agent (CP) in vitrification medium to improve… (more)

▼ The main objective of this study was to investigate if natural honey can be used as a cryoprotecting agent (CP) in vitrification medium to improve the viability of vitrified-warmed bovine oocytes. The first study was conducted to investigate the dehydration capability of natural honey compared with sucrose, and to determine the proper concentration of honey-based medium and the optimum time for sufficiently safe dehydration of bovine oocytes. Matured cumulus-oocyte complexs (COCs) were denuded and introduced individually into different concentrations (0.25, 0.5, 1.0, 1.5 or 2.0 M) of honey and sucrose-based medium followed by rehydration in control media (TCM). Video images were recorded during dehydration and rehydration, and oocyte images were captured at 12 time intervals to calculate oocyte-volume changes during dehydration and rehydration. Results demonstrated that, in honey-based media, the maximum oocyte shrinkage was achieved after 60 sec exposure in 0.25M, 0.5M and 1.0M concentrations; while at higher concentrations 1.5M and 2.0M, the maximum dehydration occurred at 30 and 20 seconds respectively. In sucrose-based medium, the maximum oocyte shrinkage was achieved after 60 sec exposure in 0.25 or 0.5M concentrations. However, at higher concentrations (1M, 1.5M or 2M), the maximum dehydration occurred at 30, 20 and 10 sec. For rehydration, oocytes dehydrated in honey or sucrose-based medium were able to regain their original volume within 60-120 sec. However, oocytes dehydrated in higher concentrations (2M honey, and 1.5M and 2M sucrose) were rehydrated back to their original volume within 20 sec. This study concluded that natural honey and sucrose caused similar cell dehydration. Only oocytes dehydrated in 1M honey-based media reached maximal dehydration after 60 sec and equally regained original volume. Therefore, 1M of honey-based medium is suggested for sufficient and safe oocyte dehydration during vitrification. The second study was conducted to determine in vitro maturation (IVM), in vitro fertilization (IVF) and embryonic development of bovine oocytes vitrified in honey-based vitrifcation media. In Experiment 1, bovine COCs were randomly distributed in control group (non-vitrified; G1), 0.5M sucrose group (second control; G2), and 0.5M, 1M and 1.5M honey groups (G3, G4 and G5 respectively). The COCs were exposed to equilibration solution 1 (VS1) at ~ 22 °C for 5 min and to vitrification solution 2 (VS2) for 1 min, mounted on Cryotops and plunged into LN2. COCs were warmed in TCM and honey/sucrose medium at 38.5 °C for 1 min, washed, matured in vitro (IVM), denuded, and immunostained to evaluate maturation. Maturation rate was significantly higher (80.7%) in control group (G1) than in vitrified groups (56, 52, 55 and 51% in G2, G3, G4 and G5, respectively) (P<.0001), whereas there was no significant difference among the vitrified groups (P>0.05). In Experiment 2, bovine COCs distributed in control (not vitrified, G1) and vitrified groups using 1M honey and 0.5M sucrose (G2 and G3 respectively),… Advisors/Committee Members: Muir, Gillian, Anzar, Muhammad, Lessard, Carl, Singh, Jaswant, Pettitt, Murray.

Subjects/Keywords: Vitrification medium; bovine oocyte; natural honey; volume changes and viability

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alfoteisy, B. (2012). Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-01-336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alfoteisy, Bilal. “Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes.” 2012. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2012-01-336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alfoteisy, Bilal. “Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes.” 2012. Web. 13 Apr 2021.

Vancouver:

Alfoteisy B. Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2012-01-336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alfoteisy B. Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-01-336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

3. Hassan, Atiq. Intermittent hypoxia induces spinal plasticity in rats with cervical spinal cord injury.

Degree: 2015, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2015-09-2235

► Many experimental therapies have been used in the search for effective approaches to improve recovery after spinal cord injury (SCI). One of the most promising… (more)

▼ Many experimental therapies have been used in the search for effective approaches to improve recovery after spinal cord injury (SCI). One of the most promising approaches is the augmentation of spontaneously occurring plasticity in uninjured neural pathways. Acute intermittent hypoxia (AIH-brief exposures to reduced O2 levels alternating with normal O2 levels) elicits plasticity in respiratory and non-respiratory spinal systems in experimental animals. AIH treatment has also been shown to improve walking abilities in persons with chronic incomplete SCI. In this thesis, I first examined the effect of AIH treatment, alone or in combination with motor training, on functional recovery in a rat model of incomplete cervical SCI. Second, I examined the effect of AIH on the expression of plasticity- and hypoxia-related proteins in the spinal cords of SCI rats. In a randomized, blinded, normoxia-controlled study, rats were trained to cross a horizontal ladder and footslip errors were measured before surgery for SCI, 4 wks post-surgery, each day of daily AIH treatment, and 1, 2, 4 and 8 weeks after treatment. dAIH treatment consisted of 10 episodes of AIH: (5 min 11% O2: 5 min 21% O2) for 7 days beginning at 4 wks post-SCI. AIH-treated rats made fewer footslips on the ladder task compared to normoxia-treated control rats after 4 days of treatment and this improvement was sustained for 8 wks post-treatment. Importantly, daily ladder training was required for AIH treatment to facilitate recovery. AIH treatment + motor training also increased the expression of Hypoxia-inducible factor-1α (HIF-1α), Vascular endothelial growth factor (VEGF), Brain-derived neurotrophic factor (BDNF), tyrosine kinase B receptors (trkB) and phospho-trkB in spinal motor neurons in SCI rats compared to normoxia-treated SCI rats. In particular these hypoxia- and plasticity-related proteins were differentially expressed both temporally and spatially in the spinal cord during AIH treatment. These findings demonstrate that AIH + motor training can augment neural plasticity and improve motor recovery in an animal model of SCI. Taken together with the promising findings from human SCI studies, the results of this thesis suggest that AIH has potential as an effective therapy to restore motor function after nervous system injury. Advisors/Committee Members: Muir, Gillian D., Machin, Karen, Weber, Lynn, Verge, Valerie, Paterson, Phyllis.

Subjects/Keywords: Intermittent hypoxia; Plasticity; Spinal cord injury

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APA (6^th Edition):

Hassan, A. (2015). Intermittent hypoxia induces spinal plasticity in rats with cervical spinal cord injury. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-09-2235

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hassan, Atiq. “Intermittent hypoxia induces spinal plasticity in rats with cervical spinal cord injury.” 2015. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2015-09-2235.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hassan, Atiq. “Intermittent hypoxia induces spinal plasticity in rats with cervical spinal cord injury.” 2015. Web. 13 Apr 2021.

Vancouver:

Hassan A. Intermittent hypoxia induces spinal plasticity in rats with cervical spinal cord injury. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2015-09-2235.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hassan A. Intermittent hypoxia induces spinal plasticity in rats with cervical spinal cord injury. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-09-2235

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

4. Cervantes, Miriam P. The rabbit as an animal model for the study of ovulation-inducing factor.

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2011-08-63

► Ovulation–inducing factor (OIF) is a seminal plasma protein that has been found in the ejaculate of different species. Evidence suggests that OIF and its function… (more)

▼ Ovulation–inducing factor (OIF) is a seminal plasma protein that has been found in the ejaculate of different species. Evidence suggests that OIF and its function may be conserved among species. Our general objective was to develop a rabbit model for the study of OIF in seminal plasma. In the first study, we conducted two experiments to validate the use of ultrasound biomicroscopy as a method for assessing ovarian structures in rabbits, and to develop a method that permits serial noninvasive repetitive ultrasound evaluation of ovarian structures in vivo in rabbits. In Experiment 1, the number and size of follicles ≥ 0.6 mm and corpora lutea (CL) detected by ultrasound biomicroscopy (UBM) ex situ were correlated with those detected by histology in each pair of ovaries from 4 female New Zealand White rabbits (5-5.5 months old) given an ovulation-inducing treatment. In Experiment 2, we translocated the ovaries of female New Zealand white rabbits (n=12; 5 months old) to a subcutaneous position in order to develop a method that permits serial UBM evaluation of ovarian structures in vivo in rabbits. Results showed strong correlations (P < 0.05) between UBM and histology in all the ovarian variables evaluated in Experiment 1, and also showed that ovarian structures are easily identified using UBM in vivo in the rabbits submitted to the surgical approach performed in Experiment 2. In the second study, we conducted two experiments to test the hypothesis that llama and rabbit seminal plasma elicits a surge of LH release and is responsible for inducing ovulation in rabbits. In Experiment 1, we compared the effect of an intramuscular administration of saline, GnRH, llama or rabbit seminal plasma in female New Zealand White rabbits (n=4-6 per group, 5.5 months old) that were group-housed. In Experiment 2, we compared the effects of the same treatments in rabbits (n=5-7 per group, 5.5 months old) that were individually caged. Ovulation and CL formation occurred in most rabbits regardless of the treatment given when animals were group housed, while rabbits given similar treatment but individually housed did not ovulate. In the GnRH group, a surge in plasma LH concentrations was observed in all the rabbits, followed by CL formation and an increase in plasma progesterone concentrations. In summary, we developed a rabbit model for the study of OIF, where ovarian structures can be evaluated in vivo by UBM. Results did not support the hypothesis that OIF in seminal plasma elicits ovulation in rabbits. Further studies are needed to determine the effects of dose and route of administration of seminal plasma in rabbits. Advisors/Committee Members: Adams, Gregg P., Muir, Gillian, Singh, Jaswant, Lessard, Carl, Baerwald, Angie.

Subjects/Keywords: Rabbit; seminal plasma; ovulation-inducing factor; ultrasound biomicroscopy; ex situ; in vivo.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cervantes, M. P. (2011). The rabbit as an animal model for the study of ovulation-inducing factor. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2011-08-63

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cervantes, Miriam P. “The rabbit as an animal model for the study of ovulation-inducing factor.” 2011. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2011-08-63.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cervantes, Miriam P. “The rabbit as an animal model for the study of ovulation-inducing factor.” 2011. Web. 13 Apr 2021.

Vancouver:

Cervantes MP. The rabbit as an animal model for the study of ovulation-inducing factor. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2011-08-63.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cervantes MP. The rabbit as an animal model for the study of ovulation-inducing factor. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/ETD-2011-08-63

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

5. Hussain, Syed. Cryopreservation of bison semen.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-04-435

► ABSTRACT The cryopreservation of bison semen is not as successful as for dairy and beef bull semen. The overall objective of this research was to… (more)

▼ ABSTRACT The cryopreservation of bison semen is not as successful as for dairy and beef bull semen. The overall objective of this research was to develop a cryopreservation procedure to improve the post-thaw survival rate of bison sperm. For this purpose two different studies were conducted. The first study consisted of two experiments. In Experiment 1, damage to bison sperm motility characteristics at different stages of cryopreservation was quantified. In Experiment 2, the effects of extenders and freeze rates on post-thaw motility and structural characteristics were investigated. In Experiment 1, semen was diluted in Triladyl extender and frozen with a freeze rate –10°C/min. Sperm motility characteristics, i.e., total motility, progressive motility, curvilinear velocity (VCL), average path velocity (VAP) and straight-line velocity (VSL) were recorded in fresh, diluted, chilled (4°C) and frozen-thawed semen using computer-assisted sperm analysis (CASA). There was a significant decline (P < 0.001) in total and progressive sperm motilities (35% and 42%, respectively) after the freeze-thaw stage compared to pre-freeze stages. All sperm velocities declined (P < 0.05) approximately 32% at dilution, and in post-thaw semen, VCL was reduced more (P < 0.05) than VAP and VSL. In Experiment 2, semen was diluted in Triladyl or TCA extender and frozen at three different freeze rates: –10, –25 and –40°C/min. Post-thaw sperm motility characteristics were assessed using CASA, and sperm structural characteristics, i.e., intact plasma membrane (IPM), mitochondrial membrane potential (∆ΨM) and intact acrosomes (IACR) were evaluated using flow cytometry, at 0 and 3 h post-thaw after incubating at 37°C. Triladyl yielded better (P < 0.05) post-thaw sperm total and progressive motilities (41% and 34%, respectively) than TCA (36% and 29%, respectively) at 0 h. However, post-thaw percent of decline in sperm motilities and structural characteristics after 3 h of incubation at 37°C, was less (P < 0.05) in TCA than in Triladyl extender. At post-thaw 0 h freeze rate did not affect any sperm characteristics, while a freeze rate of –40°C/min revealed less (P < 0.05) percent decline in total and progressive motilities (38% and 45%), than other freeze rates (average 50% and 60%, respectively), after 3 h of post-thaw incubation. In the second study, three experiments were conducted to determine the effects of zwitterion extender and temperature of glycerol addition (Experiment 1), the addition of reduced glutathione (GSH; Experiment 2) and cholesterol-loaded cyclodextrin (CLC; Experiment 3) on post-thaw quality of bison sperm. In Experiment 1, bison semen was diluted in Triladyl (control) or zwitterion-based (ZI) extenders, i.e. HepesT (Hepes-Tris) and TesT (Tes-Tris). In addition, glycerol in ZI extenders was added either at 37 or 4°C, before cryopreservation. Extenders had no significant effect (P > 0.05) on immediate (0 h) post-thaw total and progressive sperm motilities. However, sperm velocities (VCL, VAP, VSL) were better (P < 0.05) in ZI… Advisors/Committee Members: Anzar, Muhammad, Lessard, Carl, Barth, Albert, Adams, Gregg, Muir, Gillian.

Subjects/Keywords: bison; cryopreservation; semen; extender; freeze rate; glycerol; post-thaw; motility.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hussain, S. (2012). Cryopreservation of bison semen. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-04-435

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hussain, Syed. “Cryopreservation of bison semen.” 2012. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2012-04-435.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hussain, Syed. “Cryopreservation of bison semen.” 2012. Web. 13 Apr 2021.

Vancouver:

Hussain S. Cryopreservation of bison semen. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2012-04-435.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hussain S. Cryopreservation of bison semen. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-04-435

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

6. Matwee, Larisa K 1988-. EFFECTS OF PROTEIN-ENERGY MALNUTRITION ON SPONTANEOUS MOTOR RECOVERY AFTER STROKE.

Degree: 2016, University of Saskatchewan

URL: http://hdl.handle.net/10388/7563

► The development of comorbidity factors such as malnutrition may compromise functional recovery following stroke. The objectives of this study were to elucidate the effects of… (more)

▼ The development of comorbidity factors such as malnutrition may compromise functional recovery following stroke. The objectives of this study were to elucidate the effects of post-stroke protein-energy malnutrition (PEM) on infarct size, spontaneous motor recovery, and the acute phase response in the chronic period. Adult, male (12 week old) Sprague-Dawley rats were trained for at least 14 days in the Montoya staircase. Food intake was monitored daily and body weight was recorded weekly. Just prior to inducing stroke, rats were tested in the cylinder and Montoya staircase to determine baseline values for forelimb use during spontaneous exploration and skilled reaching, respectively. These animals were then subjected to photothrombotic stroke targeted to the motor cortex or sham surgery. Animals were tested in the cylinder on day 4 after surgery, before assignment to either control diet (12.5 % protein) or PEM (0.5 % protein) (n= 6-9/experimental group), and again on days 16 and 29. The staircase was abandoned for post-stroke testing because training criteria were not met. On Day 30, blood, brain, and liver were collected for biochemical or histological analysis. Feeding the low protein diet resulted in PEM as measured by decreased body weight p<0.001), food intake (p=0.016), and serum albumin (p<0.001) and increased liver lipid (p<0.001) and serum A2M (p=0.001). Both stroke (p=0.016) and PEM (p=0.001) elicited increases in the positive acute phase protein, A2M. The effect of PEM on post-stroke cylinder performance varied by specific endpoint. PEM exacerbated forelimb asymmetry during vertical exploration on Days 16 and 29 when scored by method 1 (p≤ 0.024), and this was not due to a change in infarct size (p=0.775). Scoring exploration by method 2 and initiation of exploration by first touch demonstrated similar patterns for preferred limb use after stroke, although these endpoints were not significantly affected by PEM (p≥0.301). The score for takeoff to initiate exploration was also impaired by stroke (p<0.001), but PEM had no influence (p=0.463). Termination of exploration (landing) was not influenced by stroke (p=0.332), and there was no independent effect of PEM (p=0.959). Advisors/Committee Members: Patterson, Phyllis G, Blakley, Barry R, Muir, Gillian, Farthing, Jon P, Fisher, Thomas.

Subjects/Keywords: Protein-energy Malnutrition; Stroke; Motor Recovery

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Matwee, L. K. 1. (2016). EFFECTS OF PROTEIN-ENERGY MALNUTRITION ON SPONTANEOUS MOTOR RECOVERY AFTER STROKE. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7563

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Matwee, Larisa K 1988-. “EFFECTS OF PROTEIN-ENERGY MALNUTRITION ON SPONTANEOUS MOTOR RECOVERY AFTER STROKE.” 2016. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/7563.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Matwee, Larisa K 1988-. “EFFECTS OF PROTEIN-ENERGY MALNUTRITION ON SPONTANEOUS MOTOR RECOVERY AFTER STROKE.” 2016. Web. 13 Apr 2021.

Vancouver:

Matwee LK1. EFFECTS OF PROTEIN-ENERGY MALNUTRITION ON SPONTANEOUS MOTOR RECOVERY AFTER STROKE. [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/7563.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Matwee LK1. EFFECTS OF PROTEIN-ENERGY MALNUTRITION ON SPONTANEOUS MOTOR RECOVERY AFTER STROKE. [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/7563

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

7. Palomino, Jesus Manuel. Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae).

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-08022011-164451

► For this thesis our objectives were to establish an efficient method of ovarian synchronization and superstimulation in bison, and determine the effects of gonadotropin treatments… (more)

▼ For this thesis our objectives were to establish an efficient method of ovarian synchronization and superstimulation in bison, and determine the effects of gonadotropin treatments on oocyte collection efficiency and quality in bison. In the first study we conducted two experiments to develop an efficient protocol for synchronization of follicular wave emergence during the anovulatory season. In Experiment 1, we compared the synchronizing effect of follicular ablation (n = 9) and treatment with 2 mg estradiol (E-) 17Î² in oil (n = 10), while in Experiment 2, we compared follicular ablation (n = 9) and treatment with 2 mg E-17Î² + 100 mg progesterone (P4; n = 10). Results showed that the degree of synchrony did not differ between ablation and hormone treatment groups in either Experiment, but follicular wave emergence was more synchronous in both treatment groups compared to the untreated control phase. The second study was conducted to develop an efficient method for ovarian superstimulation and oocyte collection during the anovulatory and ovulatory seasons. During the anovulatory season, one experiment was conducted in two replicates to compare the superstimulatory effect of 2500 IU of eCG (n = 10) given intramuscularly vs two doses of 200 mg of pFSH each (n = 10) given subcutaneously. Additionally, the effect of 25 mg of pLH given 24 hours prior oocyte collection on oocyte quality and collection rate was evaluated for each superstimulatory treatment. Results showed that treatment with pFSH induced a higher superstimulatory response and more cumulus oocyte complexes (COC) collected than did eCG during the anovulatory season. Furthermore, treatment with pLH increased the proportion of expanded COC that were collected with ultrasound-guided follicular aspiration. Two experiments were conducted during the ovulatory season, to develop an efficient protocol for superstimulation and oocyte collection. In Experiment 1, we compared the effect of two intramuscular doses of 200 mg of pFSH in saline (n = 11) vs two intramuscular doses of 200 mg of pFSH in a proprietary slow release formulation (SRF; n = 11). In Experiment 2, we compared the effect of a single dose of 2500 IU eCG intramuscularly vs two doses of 200 mg of pFSH administered subcutaneously. Results showed that a 2-dose regime of pFSH, diluted in either saline or a slow-release formulation induced a similar superstimulatory ovarian response in wood bison, while bison given a single-dose of 2500 IU eCG had a significantly lower ovarian response. In summary, synchronization of follicle wave emergence can be effectively accomplished in wood bison during the anovulatory season and follicular ablation, E-17Î² and E-17Î² + P4 treatments all shortened, and decreased the variability in the interval to follicular wave emergence. In addition, oocyte collection by transvaginal ultrasound-guided follicle aspiration from superstimulated bison was feasible and practical. Finally, treatment with pFSH was more effective than eCG to induce ovarian superstimulation for… Advisors/Committee Members: Adams, Gregg, Barth, Albert, Muir, Gillian, McCorkell, Robert, Mapletoft, Reuben, Woodbury, Murray.

Subjects/Keywords: wood bison; oocyte collection; superstimulation; follicular wave emergence

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APA (6^th Edition):

Palomino, J. M. (2011). Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae). (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08022011-164451

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Palomino, Jesus Manuel. “Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae).” 2011. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-08022011-164451.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Palomino, Jesus Manuel. “Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae).” 2011. Web. 13 Apr 2021.

Vancouver:

Palomino JM. Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae). [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-08022011-164451.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Palomino JM. Ovarian synchronization and superstimulation in wood bison (Bison bison athabascae). [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/etd-08022011-164451

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

8. Carmalt, James L 1973-. EQUINE PITUITARY PARS INTERMEDIA DYSFUNCTION (PPID): PATHOPHYSIOLOGY AND A SURGICAL APPROACH TO TREATMENT.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/7952

► Equine pituitary pars intermedia dysfunction (PPID) is a common endocrine disease of the older horse. First described in 1932, and likened to human Cushing’s disease,… (more)

Subjects/Keywords: Equine; PPID; Cushings Disease; Pathophysiology; Surgery; Treatment

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APA (6^th Edition):

Carmalt, J. L. 1. (2017). EQUINE PITUITARY PARS INTERMEDIA DYSFUNCTION (PPID): PATHOPHYSIOLOGY AND A SURGICAL APPROACH TO TREATMENT. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carmalt, James L 1973-. “EQUINE PITUITARY PARS INTERMEDIA DYSFUNCTION (PPID): PATHOPHYSIOLOGY AND A SURGICAL APPROACH TO TREATMENT.” 2017. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/7952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carmalt, James L 1973-. “EQUINE PITUITARY PARS INTERMEDIA DYSFUNCTION (PPID): PATHOPHYSIOLOGY AND A SURGICAL APPROACH TO TREATMENT.” 2017. Web. 13 Apr 2021.

Vancouver:

Carmalt JL1. EQUINE PITUITARY PARS INTERMEDIA DYSFUNCTION (PPID): PATHOPHYSIOLOGY AND A SURGICAL APPROACH TO TREATMENT. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/7952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carmalt JL1. EQUINE PITUITARY PARS INTERMEDIA DYSFUNCTION (PPID): PATHOPHYSIOLOGY AND A SURGICAL APPROACH TO TREATMENT. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

9. Leonardi, Carlos Eduardo 1984-. KISSPEPTIN FUNCTION IN FEMALE BOVINE REPRODUCTION.

Degree: 2018, University of Saskatchewan

URL: http://hdl.handle.net/10388/9597

► Kisspeptin is a modulator of GnRH and LH secretion in mammalian species. The regulator effect of kisspeptin on the hypothalamic-hypophysial-gonadal axis is unknown in the… (more)

▼ Kisspeptin is a modulator of GnRH and LH secretion in mammalian species. The regulator effect of kisspeptin on the hypothalamic-hypophysial-gonadal axis is unknown in the bovine species. The overall objective of the thesis was to determine the mechanism of action of kisspeptin on the GnRH-LH system and its effect on ovarian follicular dynamics in pubertal cattle. In the first study, the distribution of kisspeptin immunoreactive cells and their neuronal association with GnRH positive neurons was characterized during the proestrous, metestrous and diestrous stages of the estrous cycle in cows (n=7). Kisspeptin perikarya were distributed in two main populations in cows: a cranial cluster located in the preoptic area of the brainstem, and a caudal aggregation in the arcuate nucleus of the hypothalamus. The percentage of kisspeptin immunoreactive fibers in the preoptic area (P=0.01) and the close association with GnRH perikarya (P=0.09) increased during the low progesterone period and proestrous phase of the estrous cycle in cows respectively. In the second study, the effect of peripheral administration of the shortest form of kisspeptin (kisspeptin-10) was evaluated in sexually mature female cattle (n=52) during the luteal period of the estrous cycle. Intravenous treatment with the human kisspeptin-10 sequence enhanced the plasma LH concentration to a greater extent than intramuscular treatment (P<0.01). Kisspeptin administration induced increased LH in a dose dependent fashion (P<0.01) and increased the dominant follicular size when given at high dose (15mg) during the first ovarian follicular wave. The objective of the third study was to compare the effect of a single intravenous bolus versus multiple injections of the human versus murine kisspeptin on Luteinizing hormone secretion; furthermore, the fate of the dominant follicle was compared during the low-progesterone environment in pubertal heifers (n=72). A single bolus injection or multiple intravenous injections of human kisspeptin-10 given over 2 hours period increased LH plasma concentrations more than the respective treatments with murine kisspeptin-10 (P<0.01). Also, 45 mg of human kisspeptin-10 given over a period of two hours (3 to 9 intravenous injections) induced a similar ovulation rate as GnRH treatment (P=0.44). The mechanism of peripheral administration of kisspeptin on LH release and ovulation was elucidated in the last study (n=21). After two hours of administration, kisspeptin-10 did not enhance GnRH neuron activation in the preoptic area or the hypothalamus of cows. However, after cows were given a GnRH antagonist, kisspeptin treatment was not able to increase plasma LH concentrations and induce ovulation in pubertal heifers. This observation suggests that LH release is mediated by GnRH secretion, but may not induce de-novo GnRH synthesis after exogenous peripheral administration of kisspeptin. In summary, we have determined the mechanism of action of peripheral administration of kisspeptin on reproductive control in mature cattle. For the… Advisors/Committee Members: Singh, Jaswant, Honaramooz, Ali, Baerwald, Angela, Dias, Fernanda, Adams, Gregg, Muir, Gillian.

Subjects/Keywords: kisspeptin; cattle; estrous cycle, ovulation, ovarian follicle

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APA (6^th Edition):

Leonardi, C. E. 1. (2018). KISSPEPTIN FUNCTION IN FEMALE BOVINE REPRODUCTION. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/9597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Leonardi, Carlos Eduardo 1984-. “KISSPEPTIN FUNCTION IN FEMALE BOVINE REPRODUCTION.” 2018. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/9597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Leonardi, Carlos Eduardo 1984-. “KISSPEPTIN FUNCTION IN FEMALE BOVINE REPRODUCTION.” 2018. Web. 13 Apr 2021.

Vancouver:

Leonardi CE1. KISSPEPTIN FUNCTION IN FEMALE BOVINE REPRODUCTION. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/9597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leonardi CE1. KISSPEPTIN FUNCTION IN FEMALE BOVINE REPRODUCTION. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/9597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

10. McElroy, Dan L. Positive allosteric modulation of cannabinoid type-1 receptors: Effects of GAT211 on MK-801-induced behaviors in rats.

Degree: 2020, University of Saskatchewan

URL: http://hdl.handle.net/10388/13109

► Antipsychotics help alleviate the positive symptoms associated with schizophrenia; however, their debilitating side effects spur the search for better treatment options. Acute N-methyl-D-aspartate receptor (NMDAR)… (more)

Subjects/Keywords: schizophrenia; antipsychotic; cannabis; THC; NMDA receptor; MK-801; CB1 receptor; GAT211; positive allosteric modulation; open field; acoustic startle, prepulse inhibition, locomotor activity

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APA (6^th Edition):

McElroy, D. L. (2020). Positive allosteric modulation of cannabinoid type-1 receptors: Effects of GAT211 on MK-801-induced behaviors in rats. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/13109

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McElroy, Dan L. “Positive allosteric modulation of cannabinoid type-1 receptors: Effects of GAT211 on MK-801-induced behaviors in rats.” 2020. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/13109.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McElroy, Dan L. “Positive allosteric modulation of cannabinoid type-1 receptors: Effects of GAT211 on MK-801-induced behaviors in rats.” 2020. Web. 13 Apr 2021.

Vancouver:

McElroy DL. Positive allosteric modulation of cannabinoid type-1 receptors: Effects of GAT211 on MK-801-induced behaviors in rats. [Internet] [Thesis]. University of Saskatchewan; 2020. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/13109.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McElroy DL. Positive allosteric modulation of cannabinoid type-1 receptors: Effects of GAT211 on MK-801-induced behaviors in rats. [Thesis]. University of Saskatchewan; 2020. Available from: http://hdl.handle.net/10388/13109

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

11. Gratton, Gillian. Bison seminal plasma factor affects sperm plasma membrane integrity.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-02-359

► ABSTRACT The bison industry has been growing exponentially over the last few decades; with a 35 % increase in the number of bison within Canada… (more)

▼ ABSTRACT The bison industry has been growing exponentially over the last few decades; with a 35 % increase in the number of bison within Canada from 2001 - 2006 and bison meat selling at a record high. This growth is important for the Canadian and American economy, especially in light of recent setbacks in the beef market. Presently, bison ranchers have not made use of reproductive technologies that are commonly practiced in the dairy and beef industries. In order to optimise financial gain it would be advantageous to make use of these technologies within the bison industry. One issue that has interfered with ranchers being able to make use of reproductive technologies is; bison are wild animals being kept in captivity. This means that bison become highly stressed while being handled in close contact with humans. When stressed, the bison become unpredictable and dangerous, possibly hurting themselves and the handlers. Moreover, stressed bison are difficult to electroejaculate and the collected semen is low quality. The first objective of this study was to find a way to lower the stress in bison during handling in order to improve methods for semen collection. It was hypothesised that a long acting tranquilizer (Piportil®) would lower the stress levels in the bulls, and improved the quality of collected semen. The results of the study that make up this thesis demonstrated that the animals treated with 200 mg/bull of Piportil® were less stressed when moved through the hydraulic-controlled chute system (P < 0.05). Also, treated bulls had lowered endocrine stress indicators during handling and semen collection while testosterone production was increased for treated bulls compared to their control (corticosterone: 0.101 ± 0.01 vs. 0.145 ± 0.02 ng/mL; testosterone: 9.107 ± 1.68 vs. 5.327 ± 0.74 ng/mL) respectively (P < 0.05). Moreover, the quality of semen collected from bulls treated with 200 mg of Piportil® was significantly better when compared to untreated bulls (P < 0.05). No detrimental side effects were observed when using Piportil® on the bison bulls, allowing us to suggest that this drug provided a way of lowering level and increasing semen quality. It has been suggested, that there maybe a factor in bison seminal plasma that affects the sperm plasma membrane causing increased damage during cryopreservation; resulting in low post thaw motility. However, this factor appears to be captured in the low density lipoprotein (LDL) fraction of an animal based extender. The objective of this part of the study was to identify the bison seminal plasma proteins that were captured by the LDL in Triladyl®. Bison seminal plasma proteins that were associated with either LDL from Triladyl® or the phospholipids from Andromed® were analysed using 2 dimensional electrophoreses (2DE) (47 and 21 protein spots respectively). From these spots, 17 protein spots of interest were identified by Matrix-Associated Laser Desorption Ionization Time of Flight (MALDI-TOF). Once the protein identities were known, the main functions of… Advisors/Committee Members: Lessard, Carl, Anzar, Muhammad, McCorkell, Robert B., Barth, Albert D., Loewen, Matthew E., Muir, Gillian D., Shury, Todd K..

Subjects/Keywords: Bison; Sperm, Stress, Seminal plasma proteins

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APA (6^th Edition):

Gratton, G. (2012). Bison seminal plasma factor affects sperm plasma membrane integrity. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-02-359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gratton, Gillian. “Bison seminal plasma factor affects sperm plasma membrane integrity.” 2012. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2012-02-359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gratton, Gillian. “Bison seminal plasma factor affects sperm plasma membrane integrity.” 2012. Web. 13 Apr 2021.

Vancouver:

Gratton G. Bison seminal plasma factor affects sperm plasma membrane integrity. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2012-02-359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gratton G. Bison seminal plasma factor affects sperm plasma membrane integrity. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-02-359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Prentice, Jennifer Rae. Vitrification of bovine oocytes.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-12082010-190458

► The overall objective of this thesis was to investigate the vitrification of bovine cumulus oocyte complexes (COCs) as a tool for conservation of female genetics.… (more)

Subjects/Keywords: Bovine; Vitrification; Oocyte

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APA (6^th Edition):

Prentice, J. R. (2010). Vitrification of bovine oocytes. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12082010-190458

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prentice, Jennifer Rae. “Vitrification of bovine oocytes.” 2010. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-12082010-190458.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prentice, Jennifer Rae. “Vitrification of bovine oocytes.” 2010. Web. 13 Apr 2021.

Vancouver:

Prentice JR. Vitrification of bovine oocytes. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-12082010-190458.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prentice JR. Vitrification of bovine oocytes. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-12082010-190458

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Andrade Ramos, Rafaela. Modelling the protein-energy malnourished stroke patient.

Degree: 2013, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2013-06-1073

► Little is known about the effects of protein-energy malnutrition (PEM) developing after stroke on brain recovery. The goal of this project was to develop two… (more)

▼ Little is known about the effects of protein-energy malnutrition (PEM) developing after stroke on brain recovery. The goal of this project was to develop two experimental models in the adult rat to allow evaluation of nutritional effects on post-stroke recovery: (1) a PEM model, and (2) a photothrombotic stroke model. Experiment 1 examined the hypothesis that a diet containing either 1% or 0.5% protein will produce an acute state of mild-moderate PEM in adult rats. Male, Sprague-Dawley rats (16 wk) were trained in the Montoya staircase before being randomized to diets containing 0.5% (n=8), 1% (n=8), or 12.5% protein (n=10 [CON]) for 31d. Both low protein diets increased liver lipid content (p< 0.001) and decreased food intake (p= 0.005) and body weight (p< 0.001) compared to the 12.5% protein diet. The 0.5% protein group best mimicked the stroke patient, as judged by decreased serum albumin (p= 0.018) and an acute decrease in mean (±SEM) body weight (g) by d7 (0.5%= 424±15; 1%= 428±14; CON= 477±10; p = 0.011). Increased concentrations of the positive acute phase proteins, alpha-2-macroglobulin and alpha-1-acid glycoprotein, were greatest in the 0.5% group (p< 0.001). No differences were observed in the Montoya test on d3, 15, or 30 (p= 0.26). Values on d30 were: 0.5%= 109.5±4.4% of pre-diet performance; 1%= 97.2±5.5%; CON= 98.5±10.2%. Experiment 2 tested the hypothesis that targeted laser irradiation and 30 mg/kg of rose Bengal injection will cause an infarct in the forepaw region of the cortex with accompanying functional deficits. Male adult rats trained in the Montoya staircase were randomized to ISCHEMIA (n=15) or SHAM (n=3) surgery. A cortical infarct occurred in 86% of rats, with some misplacement and variability in volume (5.7-12.8 mm3). Forepaw impairments were confirmed by decreased performance in the staircase at d3 (34.3±7.3 % of pre-stroke performance, p<0.001) and diminished use in the cylinder test (30.3±4.0% affected limb use versus 53.9±1.93% prestroke, p< 0.001). At d30, mean recovery was incomplete in the staircase (p< 0.001). These experimental models, with additional refinements, can be used to address the hypothesis that deteriorating nutritional status after a stroke interferes with brain recovery. Advisors/Committee Members: Paterson, Phyllis G., Muir, Gillian, Whiting, Susan, Bandy, Brian.

Subjects/Keywords: Protein-energy malnutrition; rat models; photothrombotic stroke.

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APA (6^th Edition):

Andrade Ramos, R. (2013). Modelling the protein-energy malnourished stroke patient. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-06-1073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Andrade Ramos, Rafaela. “Modelling the protein-energy malnourished stroke patient.” 2013. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2013-06-1073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Andrade Ramos, Rafaela. “Modelling the protein-energy malnourished stroke patient.” 2013. Web. 13 Apr 2021.

Vancouver:

Andrade Ramos R. Modelling the protein-energy malnourished stroke patient. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2013-06-1073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Andrade Ramos R. Modelling the protein-energy malnourished stroke patient. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-06-1073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Longmuir, Nicole. Regulation of adenosine transporter and AMPA receptor subunit localization by protein kinase CK2 in rat hippocampus.

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-07182011-204304

► The control of extracellular adenosine is crucial to the regulation of synaptic transmission and neuroprotection. Equilibrative nucleoside transporters (ENTs) are highly expressed in the hippocampus… (more)

▼ The control of extracellular adenosine is crucial to the regulation of synaptic transmission and neuroprotection. Equilibrative nucleoside transporters (ENTs) are highly expressed in the hippocampus and widely accepted as critical regulators of adenosine tone. However, the mechanisms regulating the surface distribution and transport function of ENTs are largely unknown. Since ENT1 and ENT2 contain consensus sequences for phosphorylation by protein kinase CK2, and because this protein has been reported to regulate synaptic plasticity and ENT function in non-neuronal systems, the present thesis outlines the hypothesis that CK2-induced phosphorylation of ENTs is important for their cellular localization and thus the regulation of adenosine tone and synaptic transmission. Here, a functional interaction between adenosine CK2, ENTs and AMPA receptors in the hippocampus is reported. Western blot analysis shows that a variety of CK2 inhibitors (DMAT, TBB and DRB) significantly reduced the density of ENT1 and ENT2 proteins in hippocampal membrane fractions, suggesting that CK2-mediated phosphorylation of ENTs promotes their surface localization. In contrast, it was found that the ENT1 inhibitor NBTI significantly increased in the membrane localization of ENT1, relative to the control. Moreover, ENTs were found to immunoprecipitate with GluR1 and GluR2-containing AMPA receptors; and CK2 inhibitors caused a decrease in the membrane localization of GluR2 and GluR1 AMPA receptors. These results suggest a novel signaling complex linking CK2-regulated adenosine transport to AMPA receptor trafficking in the rat hippocampus. Although the physiological significance of these findings requires further investigation, this thesis provides insight into an adenosine regulation pathway that may be important for the regulation of synaptic transmission and neuroprotection in the rat hippocampus. Advisors/Committee Members: Cayabyab, Francisco, Muir, Gillian, Fisher, Thomas, Mulligan, Sean, Desautels, Michel.

Subjects/Keywords: adenosine; protein kinase CK2; GluR1; equilibrative nucleoside transporter; hippocampus; GluR2

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APA (6^th Edition):

Longmuir, N. (2011). Regulation of adenosine transporter and AMPA receptor subunit localization by protein kinase CK2 in rat hippocampus. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-07182011-204304

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Longmuir, Nicole. “Regulation of adenosine transporter and AMPA receptor subunit localization by protein kinase CK2 in rat hippocampus.” 2011. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-07182011-204304.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Longmuir, Nicole. “Regulation of adenosine transporter and AMPA receptor subunit localization by protein kinase CK2 in rat hippocampus.” 2011. Web. 13 Apr 2021.

Vancouver:

Longmuir N. Regulation of adenosine transporter and AMPA receptor subunit localization by protein kinase CK2 in rat hippocampus. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-07182011-204304.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Longmuir N. Regulation of adenosine transporter and AMPA receptor subunit localization by protein kinase CK2 in rat hippocampus. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/etd-07182011-204304

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Yang, June. Impacts of habitat and landscape characteristics on reproductive ecology of female lesser scaup (Aythya affinis) in the boreal forests of Alberta.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-12-875

► The lesser scaup (Aythya affinis, henceforth scaup) population decreased during the 1980s and has remained below conservation objectives. With two-thirds of the breeding population nesting… (more)

▼ The lesser scaup (Aythya affinis, henceforth scaup) population decreased during the 1980s and has remained below conservation objectives. With two-thirds of the breeding population nesting in boreal forests, it is imperative to understand how changes in habitat features and landscape changes could negatively impact breeding scaup; this information could also help to guide waterfowl conservation and management programs. It has been hypothesized that landscape modifications, such as agricultural and oil exploitation activities, could adversely affect scaup reproductive performance. Female scaup were collected by shooting in the boreal forest fringe of central Alberta during within the first two weeks of June 2008 and 2009, corresponding to the early laying period. Collection location was determined using a hand-held GPS device and body mass (BM, g) was recorded immediately. When scaup carcasses were dissected, ovaries were removed and weighed. Rapidly growing follicles (RGF) and oviductal follicles (OF) were subjected to radioimmunoassay to quantify amount of ovarian corticosterone (OCORT) deposited into follicles. Nest initiation date (NID) was determined by counting and subtracting the number of postovulatory follicles (POF) from collection date. Natural landscape features and habitat disturbances within 100 m, 250 m, 500 m, 1500 m, and 5000 m buffers of each scaup collection location were quantified, and then normalized using z-score transformation. General linear modeling was applied to BM, OCORT and NID across each of the five buffer sizes using a priori candidate models. Natural landscape and habitat disturbance parameters were model-averaged to obtain 85% confidence intervals to determine which habitat features best predicted BM, NID, and OCORT. Model-averaging revealed that the amount of natural water body habitats were negatively correlated to scaup OCORT at the 100 m and 500 m buffer zones, and negatively correlated to scaup BM at 1500 m and 5000 m buffer zones. A combination of natural water bodies (BOGSFENS, SWAMP, WATER, and WETLAND) were negatively correlated to NID across all buffer sizes tested, AGRIC disturbance was positively correlated to NID at the 100 m and 1500 m buffer zones, WELLS were negatively correlated to NID at the 1500 m buffer zone, while TRANSPORT showed varied responses at the 1500 m and 5000 m buffer zones. Post-hoc exploratory analyses were conducted to assess whether variation in OCORT, BM, and NID was related to habitat features operating at more than one spatial scale. This analysis revealed models incorporating more than one spatial scale were competitive when compared to the original a priori model sets at the 250 m buffer zone, with post-hoc models performing better than a priori models. This study highlights the need for a holistic approach to conservation management, considering not only local habitat characteristics and disturbances in the immediate vicinity of breeding waterfowl, but extending beyond and incorporating regional landscape attributes. Advisors/Committee Members: Machin, Karen L., Clark, Robert G., Cattet, Marc R., Muir, Gillian D..

Subjects/Keywords: lesser scaup; landscape; metabolomics; reproduction

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APA (6^th Edition):

Yang, J. (2012). Impacts of habitat and landscape characteristics on reproductive ecology of female lesser scaup (Aythya affinis) in the boreal forests of Alberta. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-12-875

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, June. “Impacts of habitat and landscape characteristics on reproductive ecology of female lesser scaup (Aythya affinis) in the boreal forests of Alberta.” 2012. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2012-12-875.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, June. “Impacts of habitat and landscape characteristics on reproductive ecology of female lesser scaup (Aythya affinis) in the boreal forests of Alberta.” 2012. Web. 13 Apr 2021.

Vancouver:

Yang J. Impacts of habitat and landscape characteristics on reproductive ecology of female lesser scaup (Aythya affinis) in the boreal forests of Alberta. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2012-12-875.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang J. Impacts of habitat and landscape characteristics on reproductive ecology of female lesser scaup (Aythya affinis) in the boreal forests of Alberta. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-12-875

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Jiao, Rubin. The effect of aging on myelinating gene expression and oligodendrocyte cell densities.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-10082010-151534

► During aging, there is a decrease both in the stability of central nervous system (CNS) myelin once formed and in the efficiency of its repair… (more)

Subjects/Keywords: oligodendrocyte; aging; myelin

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APA (6^th Edition):

Jiao, R. (2010). The effect of aging on myelinating gene expression and oligodendrocyte cell densities. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-10082010-151534

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jiao, Rubin. “The effect of aging on myelinating gene expression and oligodendrocyte cell densities.” 2010. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-10082010-151534.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jiao, Rubin. “The effect of aging on myelinating gene expression and oligodendrocyte cell densities.” 2010. Web. 13 Apr 2021.

Vancouver:

Jiao R. The effect of aging on myelinating gene expression and oligodendrocyte cell densities. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-10082010-151534.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jiao R. The effect of aging on myelinating gene expression and oligodendrocyte cell densities. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-10082010-151534

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Mumtaz, Naima. Acute and chronic adaptation of Supraoptic neurons to changes in osmolality.

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-05262011-102523

► Vasopressin (VP) is an antidiuretic hormone that is synthesized and released by osmosensitive magnocellular neurosecretory cells (MNCs) to regulate water homeostasis in the body. The… (more)

▼ Vasopressin (VP) is an antidiuretic hormone that is synthesized and released by osmosensitive magnocellular neurosecretory cells (MNCs) to regulate water homeostasis in the body. The rate and firing pattern of MNCs determines the amount of VP release, which is elevated during physiological stress particularly dehydration. During acute osmotic changes the MNCs shrink and swell due to hypertonic and hypotonic stimuli, respectively. In contrast to hippocampal neurons, which display regulatory volume increases (RVI) and regulatory volume decreases (RVD) in response to hypertonic and hypotonic stimuli, MNCs do not have compensatory mechanisms. The MNCs undergo hypertrophy as a part of their physiological structural and functional plasticity during chronic dehydration. These changes are thought to be important during long term osmotic changes for the sustained and high level releases of hormone. However, the mechanism of hypertrophy is still unclear and it is difficult to address this issue in vivo. We therefore undertook studies on acutely isolated MNCs to test hypertrophy in MNCs. We observed that acutely isolated MNCs treated with hyperosmolar solution (325 mOsmol kg-1) for 150 minutes in vitro showed hypertrophy (a 9% increase in CSA) and recovered their original size when returned to isotonic solution (295 mOsmol kg-1) for another 60 minutes. Whole cell patch clamp experiments showed a 34% increase in cell membrane capacitance following treatment with hypertonic solution for 90-150 minutes. The osmotically-evoked hypertrophic response was blocked by using a TAT (human immunodeficiency virus transactivator of transcription) peptide (TAT-NSF700) that prevents SNARE-mediated exocytotic fusion by blocking the function of NSF (N-ethylmaleimide-sensitive factor). The hypertrophic response did not appear to be altered by a scrambled version of the peptide, showing that osmotically-evoked hypertrophy depends on SNARE-mediated exocytotic fusion. The VP and OT-MNCs exposed to hyperosmolar solution for two hours showed increased immunofluorescence for L-type Ca²⁺ channels (both Cav1.2 and Cav1.3). Our data suggest that the osmotically-evoked hypertrophy is associated with an increase in the total membrane surface area due to the exocytotic fusion of intracellular granules with the plasma membrane and with increased expression of L-type Ca2+ channels. This study will be helpful in understanding of the adaptation that MNCs undergo during long term dehydration and pathological conditions that lead to increased plasma osmolality. Advisors/Committee Members: Fisher, Thomas E., Mulligan, Sean, Desautels, Michel, West, Nigel, Muir, Gillian.

Subjects/Keywords: adaptation; Magnocellular neurosecretory cells; Osmolality

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APA (6^th Edition):

Mumtaz, N. (2011). Acute and chronic adaptation of Supraoptic neurons to changes in osmolality. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-05262011-102523

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mumtaz, Naima. “Acute and chronic adaptation of Supraoptic neurons to changes in osmolality.” 2011. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-05262011-102523.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mumtaz, Naima. “Acute and chronic adaptation of Supraoptic neurons to changes in osmolality.” 2011. Web. 13 Apr 2021.

Vancouver:

Mumtaz N. Acute and chronic adaptation of Supraoptic neurons to changes in osmolality. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-05262011-102523.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mumtaz N. Acute and chronic adaptation of Supraoptic neurons to changes in osmolality. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/etd-05262011-102523

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Schneberger, David. Role of Toll-like receptor 9 in mouse lung inflammation in response to chicken barn air.

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-08182011-145823

► Lung dysfunction due to exposure to air in high intensity livestock barn operations is a common problem for workers in these facilities. Exposure to this… (more)

▼ Lung dysfunction due to exposure to air in high intensity livestock barn operations is a common problem for workers in these facilities. Exposure to this air has been linked to disorders such as chronic bronchitis, occupational asthma, organic dust toxic syndrome, and chronic cough and phlegm. These symptoms have been linked to higher levels of endotoxins in air in chicken and swine barns. However, there are many other toxic molecules such as bacterial DNA and gases capable of inducing respiratory inflammation. Bacterial molecules are recognized through highly conserved pattern recognition molecules called Toll-like receptors (TLR). While lipopolysaccharides are recognized by TLR4, bacterial unmethylated DNA binds to and signals through TLR9. As a prelude to understanding the biology of TLR9 in lung inflammation, it is important to precisely clarify their in situ expression in the lung. I determined expression of TLR9 in intact lungs from cattle, pigs, dogs, horses, mice, and humans. Two samples from normal lungs of cattle, pigs, dogs, three from horses, and two from inflamed calf lungs were tested. Five normal mouse and three normal human lungs were similarly tested as well as 5 human lungs with diagnosis of asthma. The expression was determined with multiple methods such as Western blots, immunohistology, immunogold electron microscopy and in situ hybridization. Lungs from all the species showed TLR9 expression in the bronchial epithelium, vascular endothelium, alveolar septa, alveolar macrophages, and type-II alveolar epithelial cells. Immuno-electron microscopy detected TLR9 on the plasma membrane, cytoplasm and the nucleus of various cells including macrophages. In situ hybridization demonstrated TLR9 mRNA in the bronchial epithelium, vascular endothelium, alveolar septa, alveolar macrophages, and type-II alveolar epithelial cells of mouse and human. Asthmatic human lungs showed many more inflammatory cells expressing TLR9 compared to healthy lungs. In cattle and horses, pulmonary intravascular macrophages showed robust expression of TLR9. Depletion of pulmonary intravascular macrophages in horses resulted in significant reduction in total TLR9 mRNA in the lungs. Having determined that TLR9 expression is similarly expressed on many lung cell types in mice and humans, I determined the role of TLR9 in barn air induced lung inflammation by exposing TLR9-/- and wild-type mice (6 per group) to single or multiple days (5 and 20) in a chicken barn. Each exposure was of 8 hours/day duration. The TLR9-/- mice exposed 5 and 20 times showed significant reductions in TNF-alpha and IFN-gamma expression in lung lavages as well as cellular changes consistent with reduced lung inflammation such as reductions in the number of lung neutrophils. This suggests that barn dust DNA, acting through TLR9, contributes to lung inflammation seen in response to exposure to chicken barn air. These fundamental data advance our knowledge on the cell-specific expression of TLR9 across a range of species including the humans and demonstrate… Advisors/Committee Members: Singh, Baljit, Muir, Gillian, Honaramooz, Ali, Hogaboam, Cory M., Mutwiri, George, Kirychuk, Shelley.

Subjects/Keywords: chicken barn; horse; cow; dog; pig; mouse; human; TLR9; Toll-like receptor 9

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APA (6^th Edition):

Schneberger, D. (2011). Role of Toll-like receptor 9 in mouse lung inflammation in response to chicken barn air. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08182011-145823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Schneberger, David. “Role of Toll-like receptor 9 in mouse lung inflammation in response to chicken barn air.” 2011. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-08182011-145823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Schneberger, David. “Role of Toll-like receptor 9 in mouse lung inflammation in response to chicken barn air.” 2011. Web. 13 Apr 2021.

Vancouver:

Schneberger D. Role of Toll-like receptor 9 in mouse lung inflammation in response to chicken barn air. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-08182011-145823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schneberger D. Role of Toll-like receptor 9 in mouse lung inflammation in response to chicken barn air. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/etd-08182011-145823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Dadarwal, Dinesh. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-11-792

► Hormonal environment in which follicle grows has been shown to affect the oocyte competence. Our objective was to identify factors that affect oocyte competence and… (more)

▼ Hormonal environment in which follicle grows has been shown to affect the oocyte competence. Our objective was to identify factors that affect oocyte competence and characterize the structural and functional changes induced by these factors. Fertility was compared in cattle following alterations in levels of progesterone and length of proestrus during dominant follicle growth. We hypothesized that subluteal-phase progesterone will mitigate the effect of a shorter proestrus on pregnancy rates. A shorter duration of proestrus during a fixed-time AI protocol in cattle resulted in a smaller preovulatory follicle, smaller and less functional CL with lower progesterone secretion, and lower fertility (P<0.01). A subluteal-phase progesterone milieu during ovulatory follicle growth induced higher pregnancy rates (P<0.01) and compensated for the effect of a short proestrus on pregnancy rates. Organelle behavior was characterized in oocytes obtained from follicles at different phases of dominant follicle growth. We hypothesized that ooplasmic organelles undergo changes in population and spatial distribution in a phase-specific manner. The growing phase oocytes have least area of mitochondria in contact with lipid droplets (P=0.04) and a peripheral distribution of lipids compared to an even distribution in oocytes from other phases. The regression phase oocytes showed an increase in mitochondrial number (P=0.03) and even distribution of mitochondria compared to peripheral in other phases. Moreover, oocytes from regression phase had higher (P<0.01) lipid content per unit volume of oocyte than other phases. Effect of follicular aging on nuclear maturation rates and, size and distribution of lipid droplets and mitochondria in in vivo matured oocytes were compared. We hypothesized that follicular aging after FSH starvation will result in maturation failure with accumulation of larger lipid droplets and altered distribution of mitochondria as compared to superstimulation with continued FSH support (4-d and 7-d). A 7-d FSH protocol resulted in greater proportion (P<0.01) of mature oocytes compared to other groups. FSH starvation lead to more poor quality oocytes that had ATP contents similar to short FSH group. Further, organization of mitochondria as intense and bigger clusters (P=0.01) alongwith increased size of lipid droplet (P=0.03) within the oocytes from FSH starvation group might indicate atresia. Effect of maternal aging on mitochondrial numbers, distribution and ATP content of in vivo matured bovine oocytes was studied. We hypothesized that in vivo matured oocytes from old cows will have reduced number of mitochondria, altered distribution of mitochondria and decreased the ATP content compared to those from young cows. Maternally aged oocytes had significantly less ATP content (P=0.01) although mitochondrial population and distribution pattern did not differ. Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Lessard, Carl, Tikoo, Suresh K., Muir, Gillian.

Subjects/Keywords: Oocyte maturation; follicular aging; maternal aging; bovine model

…thankful to University of Saskatchewan and Province of Saskatchewan for the financial support and… …GADVASU, Ludhiana for allowing me to pursue my PhD at University of Saskatchewan. v… …Veterinary and Animal Sciences, Ludhiana and University of Saskatchewan, Saskatoon. Without their…

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APA (6^th Edition):

Dadarwal, D. (2012). FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-11-792

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dadarwal, Dinesh. “FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.” 2012. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2012-11-792.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dadarwal, Dinesh. “FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.” 2012. Web. 13 Apr 2021.

Vancouver:

Dadarwal D. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2012-11-792.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dadarwal D. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-11-792

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Abbasi, Sepideh. The study and application of testis tissue xenografting.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-06292010-101429

► Testis tissue xenografting (TTX) provides a novel in vivo model for the study of testis function, and a previously-unavailable opportunity to produce spermatozoa in the… (more)

▼ Testis tissue xenografting (TTX) provides a novel in vivo model for the study of testis function, and a previously-unavailable opportunity to produce spermatozoa in the grafts from immature donors of diverse species. The overall objectives of this thesis were to examine a number of factors that potentially affect the outcome of TTX, and to apply TTX using immature bison and deer donors as models for endangered ungulates. The objective of the first experiment was to examine the effects of recipient mouse strain, gender and gonadal status on the outcome of TTX. Eight small fragments of neonatal porcine testis tissue (~5 mg each) were grafted under the back skin of immunodeficient mice of different strains (SCID vs. nude), gender (male vs. female), and gonadal status (intact vs. gonadectomised), using a 2×2×2 factorial design (8 groups, n = 7 mice/group). The xenografts were recovered at 8 mo post-grafting and evaluated for gross and histological attributes. Gonadectomy of the recipients did not affect any of the measured outcomes of TTX (P > 0.05), and data were pooled into four groups based on recipient strain and gender. Overall, male recipient mice had grafts with higher mean (+SEM) recovery rate (97 ± 2.3% vs. 88 ± 2.4%, P = 0.004), weight (348 ± 26.3 vs. 104 ± 27.0 mg, P < 0.001), seminiferous tubular diameter (150 ± 3.3 vs. 108 ± 5.3 mg, P < 0.001), percentage of tubules containing spermatozoa (32 ± 3.2 vs. 6 ± 1.8%, P < 0.001), elongated spermatids (13 ± 1.4% vs. 4 ± 0.8%, P < 0.001), and round spermatids (10 ± 1.2% vs. 6 ± 1.1%, P = 0.006) than female mice. Overall, SCID mice had grafts with higher recovery rate (98 ± 2.4% vs. 87 ± 2.3%, P = 0.001), average weight (292 ± 27.0 vs. 160 ± 26.3 mg, P = 0.001), tubular density (44 ± 3.3 vs. 33 ± 2.1, P = 0.02), percentage of tubular cross-sections containing spermatocytes (27 ± 3.7% vs. 13 ± 2.3%, P = 0.003) than nude mice. Among the four groups of recipients, the grafts from male SCID mice had the highest weight (P < 0.05) and percentage of tubules containing spermatozoa (P < 0.05). The objective of the second experiment was to evaluate the effect of using different numbers of donor testis tissue fragments on the outcome of TTX. Fragments of donor piglet testis tissue were grafted subcutaneously under the back skin of four groups of castrated male nude mice (n = 10/group). Each group of recipient mice received 2, 4, 8, or 16 fragments per mouse. Mice were sacrificed at 8 mo post-grafting, and xenografts were evaluated for physical growth and histological development. The relative weight of the vesicular gland (index) was also determined as a measure of bioactive androgen production by grafts in castrated recipient mice. The overall graft recovery rate was ~94% (range 86-98%) which did not differ among the groups (P > 0.05). The group of mice that received 16 testis tissue fragments had higher mean (+ SEM) graft weights (278 ± 39.4 vs. 106 ± 38.0, P = 0.02), total graft weight (2,443 ± 338.8 vs. 192 ± 76.2, P < 0.001), vesicular gland index (0.5 ± 0.06 vs. 0.1 ±… Advisors/Committee Members: Honaramooz, Ali, Mapletoft, Reuben, Adams, Gregg, Muir, Gillian, Barth, Albert, Baerwald, Angella.

Subjects/Keywords: Xenografting; immunodeficient mice; endangered animals; Testis

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APA (6^th Edition):

Abbasi, S. (2010). The study and application of testis tissue xenografting. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06292010-101429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abbasi, Sepideh. “The study and application of testis tissue xenografting.” 2010. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-06292010-101429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abbasi, Sepideh. “The study and application of testis tissue xenografting.” 2010. Web. 13 Apr 2021.

Vancouver:

Abbasi S. The study and application of testis tissue xenografting. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-06292010-101429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abbasi S. The study and application of testis tissue xenografting. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-06292010-101429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Tribulo, Paula. THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-10-766

► Three experiments were designed to gain an understanding of the role of ovulation-inducing factor (OIF) present in bovine seminal plasma. Within species, seminal plasma was… (more)

▼ Three experiments were designed to gain an understanding of the role of ovulation-inducing factor (OIF) present in bovine seminal plasma. Within species, seminal plasma was pooled from 1 to 4 ejaculates per male (n=160 bulls, n=4 llamas in Experiments 1 and 2, and n=95 bulls in Experiment 3). The volume of seminal plasma used for treatment was adjusted to a total dose of 250 µg of OIF. Experiment 1 was done to verify the bioactivity of OIF in bovine seminal plasma. Mature female llamas were assigned randomly to be treated intramuscularly (i.m.) with either 10 ml of phosphate buffered saline (PBS, negative control, n=5), 50 µg GnRH (positive control, n=5), 6 ml of llama seminal plasma (n=6) or 12 ml of bull seminal plasma (n=6). Experiment 2 was done to determine the effect of OIF in bovine seminal plasma on LH-induced ovulation and luteal development. Beef heifers with a CL and a growing follicle ≥10 mm were given a luteolytic dose of prostaglandin followed by 25 mg pLH 12 h later. Heifers were assigned randomly to three groups and given 10 ml bovine seminal plasma i.m. 12 h after pLH treatment (n=10), bovine seminal plasma i.m. within 4 h after ovulation (n=9), or no further treatment (control, n=10).Experiment 3 was done to determine the effect of OIF in bovine seminal plasma on LH release, ovulation and luteal development. Ovulation in beef heifers was synchronized using a protocol with progesterone and estradiol. Six days after ovulation, , when a mature CL and a dominant follicle of 11-13 mm diameter were expected to be present, heifers were assigned randomly to four groups (n=8 per group) using a 2-by-2 design and treated with either pLH or phosphate-buffered saline i.m., followed 12 h later by treatment with either 10 ml bovine seminal plasma or phosphate-buffered saline i.m.; i.e., LH+PBS, LH+SP, PBS+SP, and PBS+PBS groups. In all experiments, ovulation and CL development were monitored by transrectal ultrasonography. In Experiment 1, llamas were scanned daily from treatment to Day 6 after treatment, while in the other two experiments ovulations were monitored every 4 h and CL development was monitored daily until the next ovulation. Ovulation rates were compared among groups by Fisher’s exact test, and continuous data were compared among groups by ANOVA for repeated measures. Single point data were compared by ANOVA. In Experiment 1, ovulation was detected in 0/5, 4/5, 4/6, 4/6 in PBS, GnRH, llama seminal plasma, and bovine seminal plasma groups, respectively (P<0.05). No difference was detected among groups in luteal development. In Experiment 2, all ovulations in the pre-ovulation treatment group occurred within a 4 h period, while the range for other groups was 22 h (P<0.0001). No difference was detected among groups in luteal development; however, plasma progesterone concentrations tended to be greater in the heifers treated with seminal plasma post-ovulation compared to the other two groups (treatment-by-day interaction, P=0.1). In Experiment 3, ovulations were detected in 5/8, 4/8, 0/7, 0/8 in pLH,… Advisors/Committee Members: Adams, Gregg P., Mapletoft, Reuben J., Singh, Jaswant, Muir, Gillian, Barth, Albert.

Subjects/Keywords: Ovulation-inducing factor - Cattle nerve growth factor - reproduction

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APA (6^th Edition):

Tribulo, P. (2012). THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-10-766

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tribulo, Paula. “THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE.” 2012. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/ETD-2012-10-766.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tribulo, Paula. “THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE.” 2012. Web. 13 Apr 2021.

Vancouver:

Tribulo P. THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/ETD-2012-10-766.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tribulo P. THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-10-766

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

22. Bennett, Sean W. Biomechanical assessment of locomotion in two rodent models of nervous system injury.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-12222009-223111

► The adaptation of inverse dynamics was performed to quantitatively examine the subtle locomotor changes, previously undetectable, in rodent locomotion following nervous system injury. The first… (more)

Subjects/Keywords: spinal cord injury; rat locomotion; inverse dynamics; biomechanics; ground reaction forces; peripheral nerve injury; kinematics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bennett, S. W. (2009). Biomechanical assessment of locomotion in two rodent models of nervous system injury. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12222009-223111

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bennett, Sean W. “Biomechanical assessment of locomotion in two rodent models of nervous system injury.” 2009. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-12222009-223111.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bennett, Sean W. “Biomechanical assessment of locomotion in two rodent models of nervous system injury.” 2009. Web. 13 Apr 2021.

Vancouver:

Bennett SW. Biomechanical assessment of locomotion in two rodent models of nervous system injury. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-12222009-223111.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bennett SW. Biomechanical assessment of locomotion in two rodent models of nervous system injury. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-12222009-223111

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

23. Marit, Jordan Scott. Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio).

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-11082010-181618

► Examination of the swimming capabilities of fish is increasingly being considered as an effective method for determining sublethal toxicity. Acute toxicant exposure is known to… (more)

▼ Examination of the swimming capabilities of fish is increasingly being considered as an effective method for determining sublethal toxicity. Acute toxicant exposure is known to cause decreases in swim performance in fish but less is known about how developmental exposure can cause persistent effects that hinder swimming. In addition, little is known about how triglyceride levels fluctuate during fish swimming upon both acute and developmental exposure to toxicant. In this thesis, two studies, one acute and one developmental, were carried out using two different toxicants in order to address these issues. In order to examine acute effects, adult zebrafish (Danio rerio) were exposed to ethanol vehicle or increasing concentrations of 2,4-dinitrophenol (DNP), a mitochondrial electron transport chain uncoupler, for a 24 h period. Following exposure, fish were placed in a swim tunnel for critical swimming speed (Ucrit) determination and swim motion analysis. Whole body triglyceride levels were then determined. Ucrit was decreased in a concentration dependent manner in both the 6 mg/L and 12 mg/L DNP exposure groups, with 6 mg/L DNP being considered sublethal and 12 mg/L approaching the LC50. A decrease in tail beat frequency was observed and is likely the main cause for the decrease in Ucrit in the DNP exposure groups. Triglyceride levels were elevated in a concentration dependent manner in the DNP exposure groups. This increase in triglyceride stores may be due to a behavioral adaption limiting swimming capabilities or due to a direct toxic action of DNP on lipid catabolism. The second study examined whether developmental 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure would cause persistent toxic effects. Zebrafish embryos were exposed to dimethyl sulfoxide control or increasing concentrations of TCDD between 2-4 days post fertilization (dpf). At 5 dpf, cytochrome P450 1A (CYP1A) activity was determined. Fish were raised to 90 dpf with mortalities and deformities being recorded at 5 dpf, 10 dpf, and 90 dpf. At 90 dpf, fish were placed in swim tunnel and Ucrit , swimming motion, and aerobic scope (oxygen consumption rate during exercise minus oxygen consumption rate during rest) were determined. Following swimming, some fish were used for whole body triglyceride analysis while others were used for histological examination. Ucrit was shown to be decreased in the two highest sublethal TCDD exposure groups (0.1 and 1 ng/L) but not in the lowest TCDD exposure group (0.01 ng/L). The exact cause of the decrease in Ucrit is not known, but may be linked to the observed decrease in dorsal aorta diameter, an inability to mobilize triglyceride stores, behavioral adaptations limiting swimming, decreased body length, or a combination of these factors. This TCDD related defect in swimming ability is not due to any increases in gross deformity or mortality rates, nor does it appear that CYP1A induction is required to mediate the toxic effects. Thus, it appears that examination of swim performance may serve as an effective measure… Advisors/Committee Members: Weber, Lynn, Jones, Paul, Muir, Gillian, Niyogi, Som.

Subjects/Keywords: zebrafish; dioxin; triglycerides; dinitrophenol; sublethal toxicity; critical swimming speed

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Marit, J. S. (2010). Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio). (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-11082010-181618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Marit, Jordan Scott. “Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio).” 2010. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-11082010-181618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Marit, Jordan Scott. “Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio).” 2010. Web. 13 Apr 2021.

Vancouver:

Marit JS. Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio). [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-11082010-181618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Marit JS. Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio). [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-11082010-181618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

24. Gill, Sukhjit Singh. Recruitment and function of pulmonary intravascular macrophages in rats.

Degree: 2005, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-09072005-192939

► with biliary cirrhosis are highly susceptible to acute pulmonary dysfunction and suffer from hepato-pulmonary syndrome. The mechanisms of this enhanced susceptibility remain unknown. It is… (more)

▼ with biliary cirrhosis are highly susceptible to acute pulmonary dysfunction and suffer from hepato-pulmonary syndrome. The mechanisms of this enhanced susceptibility remain unknown. It is well established that pulmonary intravascular macrophages (PIMs) are present in cattle, horses, goat and sheep and increase susceptibility for lung inflammation. Species such as rat and mouse also recruit PIMs especially in a bile duct ligation model of biliary cirrhosis. The contributions of recruited PIMs to lung inflammation associated with liver dysfunction remain unknown. Therefore, I characterized a bile duct ligation (BDL) model in rats to study role of recruited PIMs in lung inflammation. First, Sprague Dawley rats were subjected to BDL (N=6) or sham surgeries (N=3) and were euthanized at 4 weeks post-surgery. Five rats were used as the controls. Lung tissues were collected and processed for histology, immunohistology, immuno-electron microscopy, enzyme-linked immunosorbant assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR). Light microscopy demonstrated normal lung morphology in sham-operated and control rats but showed septal recruitment of mononuclear cells, which were positive for anti-rat monocytes/macrophage antibody ED-1, in BDL rats (p=0.002). Immuno-electron microscopy confirmed localization of ED-1 in PIMs. BDL rats showed increased lung expression of monocyte chemoattractant protein-1 (MCP-1) protein and mRNA compared to the controls (p=0.017) but not of IL-1â, TNF-á, TGF-â and IL-10. Then, I treated BDL rats (N=5) with gadolinium chloride (GC; 10 mg/Kg body weight intravenous) and found reduced numbers of PIMs (p=0.061) at 48 hours post-treatment along with increased expression of TGF-â and IL-10.I challenged control rats (N=5) and BDL rats (N=6) with Escherichia coli lipopolysaccharide (E. coli LPS; 0.1 mg/Kg body weight intravenous). All the BDL rats died within 3 hours of LPS challenge (100% mortality) while the normal LPS-treated rats were euthanized at 6 hours post-treatment. Histology and ED-1 staining showed dramatic increase in the number of septal monocytes/macrophages in BDL+LPS rats compared to normal LPS-treated rats (p=0.000). Staining of lung sections with an LPS antibody localized the LPS in lungs. RT-PCR analyses showed no differences in IL-1â transcript levels between LPS challenged BDL rats and LPS challenged control rats (p=0.746) but ELISA showed increase in IL-1â concentration in LPS challenged BDL rats compared to LPS challenged control rats (p=0.000). TNF-á mRNA (p=0.062) and protein (p=0.000) was increased in BDL+LPS rats compared to the control+LPS rats. Immuno-electron microscopy showed IL-1â and TNF-á in PIMs. BDL rats challenged with LPS showed increased expression of IL-10 mRNA and protein (p=0.000 & 0.002 respectively) in lungs compared to LPS challenged control rats. TGF-â mRNA did not change (p=0.128) but lower protein concentrations (p=0.000) were observed in LPS-treated control rats compared to BDL+LPS. To further address the role of PIMs, I treated rats… Advisors/Committee Members: Singh, Baljit, Muir, Gillian D., Lohmann, Katharina, Duke, Tanya.

Subjects/Keywords: bile duct ligation; pulmonary intravascular macrophage; lung inflammation; cirrhosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gill, S. S. (2005). Recruitment and function of pulmonary intravascular macrophages in rats. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-09072005-192939

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gill, Sukhjit Singh. “Recruitment and function of pulmonary intravascular macrophages in rats.” 2005. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-09072005-192939.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gill, Sukhjit Singh. “Recruitment and function of pulmonary intravascular macrophages in rats.” 2005. Web. 13 Apr 2021.

Vancouver:

Gill SS. Recruitment and function of pulmonary intravascular macrophages in rats. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-09072005-192939.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gill SS. Recruitment and function of pulmonary intravascular macrophages in rats. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-09072005-192939

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

25. Poulton, Nadine P. The effects of treadmill training in hemi-parkinsonian rats.

Degree: 2004, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-08302004-112427

► The purpose of this study was to investigate whether locomotor training, in the form of treadmill training, could ameliorate neurochemical changes and behavioural deficits in… (more)

▼ The purpose of this study was to investigate whether locomotor training, in the form of treadmill training, could ameliorate neurochemical changes and behavioural deficits in the 6-hydroxydopamine (6-OHDA) rat model of Parkinson’s disease. It has been recently demonstrated that rehabilitative forelimb motor training can attenuate dopamine loss and some deficits in forelimb movements in this animal model. In addition, brief locomotor treadmill training has been shown to attenuate forelimb deficits in 6-OHDA treated rats. However, it is not known whether locomotor training could result in an amelioration of locomotor deficits in these animals. Rats were lesioned with 6-OHDA injected intracerebrally. Lesioned rats were randomly assigned to one of 3 groups: early treadmill trained, late treadmill trained and untrained. Animals in the treadmill groups were trained to trot on a moving treadmill for 2 x 20 minute sessions daily for 30 days, beginning either 24 hours or 7 days after 6-OHDA injection. Untrained animals were exposed to a stationary treadmill for the same time periods. All animals were assessed on their abilities to perform several behavioural tasks designed to test locomotor and forelimb movement abilities prior to 6-OHDA injection and again at 3 and 6 weeks post-injection. These tests included measurement of ground reaction forces during overground locomotion, paw placements during a ladder crossing task, forelimb useage during exploratory behaviour and ability to initiate forelimb stepping movements. In addition, assessments of dopamine depletion in the striatum were carried out first in vivo, by measuring apomorphine-induced rotations at 2 weeks post 6-OHDA injection, and subsequently by post-mortem analysis of dopamine levels in the striatum using HPLC at the conclusion of the study. Treadmill training resulted in attenuation of dopamine depletion compared to non-treadmill trained animals, as measured by both apomorphine injection and HPLC. However, treadmill training produced no difference in behavioural deficits on a variety of tests compared to untrained animals. In some cases, early treadmill trained animals tended to display more severe behavioural deficits compared to untrained animals. Late treadmill training had a similar but smaller effect compared to early treadmill training. We conclude that treadmill training does not ameliorate locomotor deficits, in the 6-OHDA model of Parkinson’s disease, even though this same training results in attenuation of dopamine loss in the striatum. Advisors/Committee Members: Muir, Gillian D., Singh, Baljit, Schreyer, David, Janz, David M..

Subjects/Keywords: neurodegenerative; treadmill training; Behaviour; Dopamine; Parkinson's disease; 6-OHDA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Poulton, N. P. (2004). The effects of treadmill training in hemi-parkinsonian rats. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08302004-112427

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Poulton, Nadine P. “The effects of treadmill training in hemi-parkinsonian rats.” 2004. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-08302004-112427.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Poulton, Nadine P. “The effects of treadmill training in hemi-parkinsonian rats.” 2004. Web. 13 Apr 2021.

Vancouver:

Poulton NP. The effects of treadmill training in hemi-parkinsonian rats. [Internet] [Thesis]. University of Saskatchewan; 2004. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-08302004-112427.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Poulton NP. The effects of treadmill training in hemi-parkinsonian rats. [Thesis]. University of Saskatchewan; 2004. Available from: http://hdl.handle.net/10388/etd-08302004-112427

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

26. Kaur, Navjot. Immune response in Rhodococcus equi infected foals.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-03172010-152826

► Rhodococcus equi (R. equi) is an intracellular, gram-positive coccobacillus that causes pneumonia in foals aged 2 to 4 months. Neonatal foals are susceptible to R.… (more)

▼ Rhodococcus equi (R. equi) is an intracellular, gram-positive coccobacillus that causes pneumonia in foals aged 2 to 4 months. Neonatal foals are susceptible to R. equi infection probably due to inefficient Toll-like receptor (TLR)-2 signaling and inability to produce interferon gamma. One of the reasons for inefficient receptor signaling and recognition of R. equi by the foal’s immune system may be the inefficient sequestration of TLRs in lipid rafts, which act as signaling platforms. However, there are no protocols to isolate lipid rafts from equine cells and, therefore, no data on the association of TLRs with the lipid rafts in the lung cells of normal and infected foals. Because of the clinical importance of the disease, there is considerable interest in developing effective prophylactic methods, which in turn requires a better understanding of fundamental immunology of the foals. In this study, I have examined the effect of R. equi vaccination on the lung inflammation induced following challenge with R. equi. I also developed a protocol to isolate lipid rafts from broncho-alveolar lavage (BAL) cells and investigated the association of lipid rafts with TLRs. In the first study, 15 mixed breed draft-type foals up to 7 weeks of age were studied. The foals were divided into control (n=7) and a vaccinated (n=8). The control foals were given 10 mL phosphate buffered saline intramuscularly while the vaccinated group was vaccinated on day 0 of the study followed by a booster on day 14. All the foals were challenged with R. equi (5x106 cells/mL into the dorso-caudal region of the right lung lobe). BAL was performed on day 14, 28 and 35 and all the foals were euthanized on day 49 of the study. The study design did not leave any non-infected foal at the end of the experiment. Therefore, lung samples were obtained from two untreated control (non-vaccinated non-infected) foals from the Department of Veterinary Pathology, University of Saskatchewan were used. The data showed similar levels of lung inflammation in both the control and vaccinated foal groups based on BAL cytology, gross pathology and histopathology. Gross and histopathological studies indicated that both control and vaccinated foals developed granulomatous lesions. Immunohistology showed increased expression of TLR4, TLR2 and TNF alpha in alveolar septa and in some cases in the vascular endothelium and airway epithelium in the lungs of both groups compared to the untreated control foals. Western blots showed increased expression of TLR2 but not TLR4 in the lung extracts from both the vaccinated and the control foals. Vaccinated foals showed higher concentrations of TNF alpha(p=0.0219) in their BAL on day 28 but lower concentrations of IL-10 (p=0.0172) in their lung extracts collected on day 49 compared to the controls. There were no differences in IFN gamma and protein concentrations between the two groups. To understand the role of lipid rafts in TLR4 and TLR2 signaling, I developed an efficient and simpler protocol to isolate lipid rafts from BAL cells of… Advisors/Committee Members: Singh, Baljit, Lohmann, Katharina, Muir, Gillian, Townsend, Hugh.

Subjects/Keywords: Rhodococcus equi; foals; cytokine; immunity; Toll lioke receptor 4; Toll like receptor 2; inflammation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kaur, N. (2010). Immune response in Rhodococcus equi infected foals. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-03172010-152826

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kaur, Navjot. “Immune response in Rhodococcus equi infected foals.” 2010. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-03172010-152826.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kaur, Navjot. “Immune response in Rhodococcus equi infected foals.” 2010. Web. 13 Apr 2021.

Vancouver:

Kaur N. Immune response in Rhodococcus equi infected foals. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-03172010-152826.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kaur N. Immune response in Rhodococcus equi infected foals. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-03172010-152826

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

27. Mahmoodzadeh Toosi, Behzad. Characteristics of FSH peaks and antral follicular wave dynamics in sheep.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-11052009-160908

► In the ewe, one to three antral follicles emerge or grow from a pool of small antral follicles (1 to 3 mm in diameter) every… (more)

▼ In the ewe, one to three antral follicles emerge or grow from a pool of small antral follicles (1 to 3 mm in diameter) every 3 to 5 days and reach diameters of ¡Ý5 mm before regression or ovulation. Each follicular wave is triggered by a peak in serum concentrations of FSH. It is not clear what characteristics of an FSH peak cause follicular wave emergence and what aspects of development of a follicular wave are regulated by its preceding FSH peak. In Experiment 1, we found that the amplitude of FSH peaks decreased, while basal serum FSH concentrations increased across the inter-ovulatory interval (P < 0.05). However, there were no associated changes in the growth, static or regression phases of follicular waves or the number and size of follicles in a wave. In Experiment 2, using computer-assisted quantitative echotextural analysis, we found that the numerical pixel value (NPV) for the wall of anovulatory follicles emerging in the third wave of the cycle was significantly higher than for waves 1 and 2 at the time of wave emergence but it decreased as follicles reached maximum follicular diameter (P < 0.05). A tendency for a similar pattern for the wall of follicles in the last wave of the cycle was also observed (P = 0.07). In Experiment 3, treatment with ovine FSH (oFSH) increased the amplitude of an FSH peak by 5 to 6 fold. This treatment increased estradiol production (P < 0.05) but had little effect on other characteristics of the subsequent follicular wave. Daily injections of oFSH (Experiment 4) for four days, resulted in the occurrence of 4 discrete peaks in serum FSH concentrations. Each injection of oFSH resulted in the emergence of a new follicular wave. In Experiment 5, six cyclic ewes received oFSH (0.1 ¦Ìg/kg, sc) every 6 h for 42 h, to try to give a gradual increase in the leading slope of an FSH peak. Serum FSH concentrations increased in oFSH treated ewes (P < 0.05) resulting in an additional peak between two endogenously driven FSH peaks and therefore, did not give the planned gradual leading slope to an FSH peak. Ovine FSH treatment occurred in the early growth phase of wave 1 of the inter-ovulatory interval and increased the growth rate of growing follicles in that wave, compared to control ewes (P < 0.05). This apparently induced dominance in follicles in wave 1, causing them to suppress wave emergence in response to the injected FSH. In Experiment 6, oFSH was infused constantly (1.98 ¦Ìg/ewe/h, iv, n = 6) for 60 h. Infusion of oFSH maintained serum FSH concentrations at a level similar to the zenith of a peak. This resulted in a superstimulatory effect with a peak in the mean number of large follicles on Day 2 after the start of FSH infusion (P < 0.001). A hormonal milieu similar to low serum progesterone concentrations was created by treatment of ewes with prostaglandin and medroxyprogesterone acetate (MAP) sponges (Experiment 7). This treatment delayed regression of the penultimate follicular wave of a cycle. However, the delayed follicular atresia was accompanied by a greater degree of… Advisors/Committee Members: Rawlings, Norman C., Misra, Vikram, Singh, Baljit, Honaramooz, Ali, Muir, Gillian, Wheaton, Jonathan.

Subjects/Keywords: Follicular Wave Dynamics; Follicle Stimulating Hormone; Sheep

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mahmoodzadeh Toosi, B. (2009). Characteristics of FSH peaks and antral follicular wave dynamics in sheep. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-11052009-160908

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mahmoodzadeh Toosi, Behzad. “Characteristics of FSH peaks and antral follicular wave dynamics in sheep.” 2009. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-11052009-160908.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mahmoodzadeh Toosi, Behzad. “Characteristics of FSH peaks and antral follicular wave dynamics in sheep.” 2009. Web. 13 Apr 2021.

Vancouver:

Mahmoodzadeh Toosi B. Characteristics of FSH peaks and antral follicular wave dynamics in sheep. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-11052009-160908.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mahmoodzadeh Toosi B. Characteristics of FSH peaks and antral follicular wave dynamics in sheep. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-11052009-160908

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

28. McCorkell, Robert Bruce. Endogenous and exogenous control of ovarian dynamics in wapiti.

Degree: 2006, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-07062006-153821

► A series of studies were guided by the principal hypothesis that it was necessary to characterize ovarian function during the seasons of the annual reproductive… (more)

▼ A series of studies were guided by the principal hypothesis that it was necessary to characterize ovarian function during the seasons of the annual reproductive cycle in wapiti and that from this knowledge novel methods of exogenous control of ovarian function would be possible. To augment existing knowledge about endogenous control of ovarian function in wapiti studies were conducted to characterize ovarian follicle dynamics during the estrous season and to characterize ovarian follicular dynamics during the periods of transition into and out of the breeding season. The third study was designed to characterize ovarian follicle development and ovulation synchrony subsequent to an estrous synchronization protocol used commercially. To evaluate novel methods of exogenous control of ovarian function studies were conducted to determine if follicular wave emergence could be electively induced using hormonal or surgical treatments to evaluate novel ovarian superstimulatory treatment protocols. It was concluded from the studies of ovarian function that follicle development during the breeding season was characterized by the regular and synchronous development of follicular waves and that 2, 3, or 4 waves occurred during each interovulatory interval. Transition into the breeding season was preceded by one short interovulatory interval (9 days) characterized by one wave of follicle development and a small, short-lived and hypo-functional corpus luteum. The last estrous cycle of the breeding season was similar to estrous cycles during the rut (21 days), but and transition to anestrus was marked by a failure of the dominant follicle to ovulate after luteal regression. The treatment protocol used commercially for estrous synchronization was effective, but unnecessarily long. It was concluded from the studies on exogenous control of ovarian function that follicular wave emergence could be electively induced using steroid hormones or follicle ablation and may be useful for estrus synchronization and superstimulatory protocols. The tested superstimulatory treatments were effective and had the advantage of reducing the treatment period by 6 days and the number of times the animals are handled by one third over a more conventional method. However, oocyte and embryo quality were not evaluated. As a result of the studies conducted and one previous study during the anouvlatory season follicle and luteal dynamics are now known in wapiti for all seasons of the year and this knowledge will provide a template upon which other species of deer can be compared. The final two studies support the principal hypothesis. The novel methods of exogenous ovarian control tested increase the potential for success when applying reproductive technologies and the successful application of these methods in wapiti should lead to their successful use in other species of deer. Advisors/Committee Members: Adams, Gregg P., Woodbury, Murray R., Muir, Gillian D., Mapletoft, Reuben J., Flood, Peter F..

Subjects/Keywords: ovary; follicle; cervid; follicle wave; estrous synchronization

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McCorkell, R. B. (2006). Endogenous and exogenous control of ovarian dynamics in wapiti. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-07062006-153821

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McCorkell, Robert Bruce. “Endogenous and exogenous control of ovarian dynamics in wapiti.” 2006. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-07062006-153821.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McCorkell, Robert Bruce. “Endogenous and exogenous control of ovarian dynamics in wapiti.” 2006. Web. 13 Apr 2021.

Vancouver:

McCorkell RB. Endogenous and exogenous control of ovarian dynamics in wapiti. [Internet] [Thesis]. University of Saskatchewan; 2006. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-07062006-153821.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McCorkell RB. Endogenous and exogenous control of ovarian dynamics in wapiti. [Thesis]. University of Saskatchewan; 2006. Available from: http://hdl.handle.net/10388/etd-07062006-153821

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

29. Han, Juying. Changes in proteoglycans in endothelial cells under hyperglycemic conditions.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-12012009-114830

► Heparan sulfate proteoglycan (HSPG) or heparan sulfate (HS) degradation may contribute to endothelial cell (EC) dysfunction in diabetes. HSPGs, syndecan and perlecan, contain a protein… (more)

▼ Heparan sulfate proteoglycan (HSPG) or heparan sulfate (HS) degradation may contribute to endothelial cell (EC) dysfunction in diabetes. HSPGs, syndecan and perlecan, contain a protein core with mainly HS glycosaminoglycans (GAGs) attached. HSPGs modulate growth factors and function in membrane filtering. Heparanase induction is likely responsible for diabetic HS degradation. Heparin protects endothelium and insulin regulates glucose metabolism. Our objectives were to observe HSPG changes by studying EC GAG content and gene expression of syndecan, perlecan and heparanase under hyperglycemic conditions with insulin and/or heparin treatment. GAGs, including HS, were determined by the carbazole assay and visualized by agarose gel electrophoresis in porcine aortic EC cultures treated with high glucose (30 mM) and/or insulin (0.01 U/ml) for 24, 48 and 72 hours and/or heparin (0.5 µg/ml) for 72 hours. High glucose decreased cell GAGs and increased medium GAGs. GAGs increased with time in control cultures and in high glucose plus insulin treated medium. GAGs were decreased with insulin but increased with insulin or heparin plus high glucose. Confluent cultured human aortic ECs were incubated with control medium, high glucose and/or insulin and/or heparin for 24 hours. Real time PCR determination showed that: high glucose increased heparanase, decreased syndecan and had no effect on perlecan mRNA; insulin or heparin with/without high glucose decreased and insulin and heparin with high glucose increased heparanase mRNA; heparin and insulin with high glucose increased but insulin decreased syndecan mRNA. Actinomycin D (10 µg/ml) inhibited heparanase and syndecan mRNA with high glucose plus insulin plus heparin and inhibited heparanase mRNA with high glucose compared to time 0 but not â-actin after addition for 0, 2, 4, 8 and 24 hours. Bioinformatic studies revealed that transcription factor Sp1 activates heparanase promoter by high glucose and may play a role in regulation of perlecan and syndecan promoters. Insulin or heparin inhibited the reduction in EC GAGs and syndecan mRNA and induction in heparanase by high glucose, indicating their protective effect. Decreased GAGs by insulin may relate to the pathology of hyperinsulinemia. Transcriptional regulation by heparin and/or insulin may cause variation in gene expression of heparanase, syndecan and perlecan. Advisors/Committee Members: Hiebert, Linda, Muir, Gillian, Lautt, Wayne, Wu, Lily, Roesler, Bill, Forsyth, George.

Subjects/Keywords: HPLC; HS disaccharides; gene expression; perlecan; diabetic cardiovascular complications; heparanase; syndecan; real time PCR; mRNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Han, J. (2009). Changes in proteoglycans in endothelial cells under hyperglycemic conditions. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12012009-114830

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Han, Juying. “Changes in proteoglycans in endothelial cells under hyperglycemic conditions.” 2009. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-12012009-114830.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Han, Juying. “Changes in proteoglycans in endothelial cells under hyperglycemic conditions.” 2009. Web. 13 Apr 2021.

Vancouver:

Han J. Changes in proteoglycans in endothelial cells under hyperglycemic conditions. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-12012009-114830.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Han J. Changes in proteoglycans in endothelial cells under hyperglycemic conditions. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-12012009-114830

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

30. Yapura, Jimena. Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-08212009-094306

► Two studies were designed to characterize the effects of a non-steroidal aromatase inhibitor, letrozole, on ovarian function in cattle. The specific objective was to test… (more)

Subjects/Keywords: Ovarian follicles; Letrozole; Aromatase inhibitors; Bovine reproduction; Estrous synchronization

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yapura, J. (2009). Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08212009-094306

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yapura, Jimena. “Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle.” 2009. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-08212009-094306.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yapura, Jimena. “Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle.” 2009. Web. 13 Apr 2021.

Vancouver:

Yapura J. Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-08212009-094306.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yapura J. Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-08212009-094306

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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