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University of Saskatchewan
1.
Yarokhno, Yaroslav.
Diagnosis and vaccination for Bovine Genital Campylobacteriosis in beef heifers.
Degree: 2015, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2015-10-2318
► Bovine Genital Campylobacteriosis is characterized by early pregnancy loss and temporary infertility in cattle. The purpose of this project was to compare diagnostic approaches to…
(more)
▼ Bovine Genital Campylobacteriosis is characterized by early pregnancy loss and temporary infertility in cattle. The purpose of this project was to compare diagnostic approaches to detect Campylobacter fetus subsp. venerealis and evaluate the efficacy of vaccination for Bovine Genital Campylobacteriosis. This thesis describes the results of two studies that compared different sample preparation methods for bovine vaginal mucus for real-time PCR and assessed a commercial vaccine in preventing infection and reproductive loss.
The first study compared real-time PCR utilizing different bovine vaginal mucus sample preparation techniques to direct culture. The magnetic bead based protocol demonstrated higher sensitivity (48.4%, P=0.02) and lower specificity (78.9%, P=0.01) than the heat lysis protocol which involved an additional dilution step (Sens=29.4%, Spec=88.2%), but did not differ from the heat lysis protocol without sample dilution (Sens=35.0%, P=0.16; Spec=81.1%, P=0.62). The sample preparation method, designed for bovine preputial samples (Chaban et al. 2012. Can J of Vet Res; 76: 166), did not work well for vaginal mucus. All modifications of that method and magnetic bead based extraction technique had low sensitivity compared to culture probably due to the biophysical properties of vaginal mucus, which could cause loss of targeted DNA during processing, or repeated sample freezing and thawing. Release of DNA directly from vaginal mucus by a modified heat lysis protocol with consequent real-time PCR could be a promising rapid screening approach after validating on fresh samples.
The second study compared the risk of infection and reproductive failure in heifers, vaccinated with a commercial multivalent vaccine containing C. fetus antigen, to heifers vaccinated with a comparable product without C. fetus, that were exposed to infected bulls. There was no significant difference between groups either in risk of Campylobacter fetus subsp. venerealis isolation (P>0.17) or in the proportion of heifers that cultured positive at least once (P=0.42), as well as in the median number times of cultured positive samples (P=0.24) and the time to first cultured positive (P=0.67). There was no difference by treatment in the weekly proportions of heifers diagnosed pregnant by either ultrasound (P>0.31) or serum concentration of pragnancy specific protein B (P>0.31) during the study, as well as in the time to first pregnancy for heifers ever diagnosed as pregnant (P=0.30) and those that remained pregnant at the end of the study (P=0.70). Similarly, the difference was not detected by treatment in the proportion of animals, ever detected pregnant during the study (P=0.57) and in pregnancy loss rates (P=0.28). However, heifers that aborted were 4 times more likely to be cultured positive than those that did not abort (P=0.01). Heifers that were not pregnant at the end of the study cultured positive 1.5 times more often than pregnant animals in treatment group (P=0.04), while in control group such difference was 4 times (P=0.01).…
Advisors/Committee Members: Hendrick, Steve H., Waldner, Cheryl L., Hill, Janet E..
Subjects/Keywords: Bovine Genital Campylobacteriosis; Campylobacter fetus venerealis; real-time PCR; vaccination
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APA ·
Chicago ·
MLA ·
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APA (6th Edition):
Yarokhno, Y. (2015). Diagnosis and vaccination for Bovine Genital Campylobacteriosis in beef heifers. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-10-2318
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yarokhno, Yaroslav. “Diagnosis and vaccination for Bovine Genital Campylobacteriosis in beef heifers.” 2015. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2015-10-2318.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yarokhno, Yaroslav. “Diagnosis and vaccination for Bovine Genital Campylobacteriosis in beef heifers.” 2015. Web. 23 Jan 2021.
Vancouver:
Yarokhno Y. Diagnosis and vaccination for Bovine Genital Campylobacteriosis in beef heifers. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2015-10-2318.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yarokhno Y. Diagnosis and vaccination for Bovine Genital Campylobacteriosis in beef heifers. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-10-2318
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
2.
Joy, Faustin 1980-.
Effect of energy source, timing of provision, and days on feed on feed efficiency of finishing beef cattle.
Degree: 2019, University of Saskatchewan
URL: http://hdl.handle.net/10388/12514
► The objective of this research was to evaluate if the decline in gain-to-feed ratio (G:F) during finishing in beef cattle could be due to a…
(more)
▼ The objective of this research was to evaluate if the decline in gain-to-feed ratio (G:F) during finishing in beef cattle could be due to a reduction in nutrient digestion, short chain fatty acid absorption, or post-absorptive nutrient utilization, and those responses were influenced by the dietary energy source or timing of provision. A high-lipid byproduct pellet (HLP) was used as a partial replacement for barley grain in a high concentrate finishing diet to partially replace starch with lipid as the energy source. Yearling steers were used for performance evaluations and ruminally cannulated Hereford crossbred heifers were used for nutrient metabolism studies. The dry matter (DM), organic matter (OM), crude protein (CP) and neutral detergent fibre (NDF) digestibility of HLP diets were lower than the barley-based control diet (CON). Utilizing a phase-feeding strategy and partially replacing barley grain and canola meal with a high-fibre high-lipid byproduct pellet in the latter part of the finishing period may improve carcass yield grade without affecting average daily gain and G:F. With advancing days on feed, diet DM digestibility (P = 0.02) and insulin resistance (P = 0.04) increase without changes in ruminal pH and plasma metabolite clearance rates. A marginal increase in forage inclusion with the HLP diet increased ADG (P = 0.04). Increasing dietary lipid supply up to 6% of DM using HLP did not affect the digesta flow and rumen fermentation parameters, therefore, increased lipid content is not associated with reduced feed efficiency of the HLP diet. In conclusion, decreasing feed efficiency in the later stages of finishing in beef cattle is most likely due to changes in the post absorptive nutrient metabolism, and these changes are not influenced by the dietary energy source. The small particle size of feed ingredients within the byproduct pellet may be the factor associated with decreased feed efficiency of HLP pellet rather than lipid content.
Advisors/Committee Members: Penner, Greg, Buchanan, Fiona, McKinnon, John, Hendrick, Steve, Burciaga-Robles, Luis.
Subjects/Keywords: Feed efficiency; Beef cattle
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Joy, F. 1. (2019). Effect of energy source, timing of provision, and days on feed on feed efficiency of finishing beef cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12514
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Joy, Faustin 1980-. “Effect of energy source, timing of provision, and days on feed on feed efficiency of finishing beef cattle.” 2019. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/12514.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Joy, Faustin 1980-. “Effect of energy source, timing of provision, and days on feed on feed efficiency of finishing beef cattle.” 2019. Web. 23 Jan 2021.
Vancouver:
Joy F1. Effect of energy source, timing of provision, and days on feed on feed efficiency of finishing beef cattle. [Internet] [Thesis]. University of Saskatchewan; 2019. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/12514.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Joy F1. Effect of energy source, timing of provision, and days on feed on feed efficiency of finishing beef cattle. [Thesis]. University of Saskatchewan; 2019. Available from: http://hdl.handle.net/10388/12514
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
3.
Amat, Samat.
Evaluation of sulfur and thiamine metabolism, short chain fatty acid absorption, and mineral status in beef cattle fed high dietary sulfur.
Degree: 2012, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2012-12-838
► A small pen study, a metabolic trial and a field observation study were conducted to evaluate sulfur (S) and thiamine metabolism, short chain fatty acid…
(more)
▼ A small pen study, a metabolic trial and a field observation study were conducted to evaluate sulfur (S) and thiamine metabolism, short chain fatty acid (SCFA) absorption, and mineral status in beef cattle fed high dietary S. In the small pen study, the effect of feeding corn (CDDGS), wheat (WDDGS) or a 50:50 corn:wheat blend (BDDGS) dried distillers grains with solubles (DDGS) on serum sulfate level of feedlot steers was evaluated using 288 crossbred steers (273.9 ± 18.5kg) in a completely randomized design. The steers were backgrounded and finished. The control backgrounding diet consisted of 34.3% barley grain, 26.0% brome grass hay, 10.3% barley straw, 22.8% barley silage, and 6.7% supplement (DM). For the three treatment diets, 17% of the barley grain was replaced with DDGS. Sulfur concentrations of control, BDDGS, CDDGS and WDDGS diets in the backgrounding phase were 0.2, 0.23, 0.31 and 0.33% (DM), respectively. The control finishing diet was 86.8% barley grain, 5.8% supplement and 7.4% barley silage (DM), and the three DDGS treatments included replacement of 40% of barley grain with an equal amount of DDGS. The corresponding sulfur concentrations for control, BDDGS, CDDGS and WDDGS diets in were 0.2, 0.33, 0.51 and 0.65% (DM), respectively. Corn DDGS or WDDGS cattle exhibited higher (P < 0.01) serum sulfate levels than BDDGS or control cattle in both backgrounding and finishing phases. Mean serum sulfate concentrations in cattle fed WDDGS and CDDGS were lower (P < 0.01) in finishing phase relative to backgrounding phase despite the higher S intake (P < 0.01).
In the metabolic study, effects of dietary S concentration and forage-to-concentrate ratio (F:C) on S and thiamine metabolism, SCFA absorption, and mineral status were evaluated using 16 ruminally cannulated heifers (initial BW 628 ± 48 kg) in a randomized complete block design with a 2 × 2 factorial treatment arrangement with main effects of dietary S (LS = 0.3% vs. HS = 0.67%, DM) and F:C (Low F:C = 4% vs. High F:C = 51% barley silage, DM). There was no interaction between S concentration and the F:C. The HS cattle had reduced DMI (P < 0.001) and SCFA (acetate, propionate and butyrate) absorption (P < 0.05) but greater concentrations of ruminal hydrogen sulfide (H2S) (P < 0.01), serum sulfate (P < 0.01) and urinary sulfate (P < 0.01), as well as greater urinary sulfate excretion (P < 0.01) than LS cattle. Free thiamine, thiamine monophosphate (TMP) and thiamine pyrophosphate (TPP) concentrations in blood and rumen fluid did not vary (P > 0.05) among HS and LS cattle. Concentration of TPP was increased by 9.2% (P = 0.10) but with a concomitant numerical decrease in free thiamine in HS brains than LS brains. The HS brains had greater TMP (P = 0.01) and total thiamine (free thiamine + TMP + TPP) (P < 0.01) than LS brains. The HS cattle had reduced (P < 0.05) ruminal cobalt (Co), iron (Fe) and manganese (Mn) but similar (P > 0.05) copper (Cu), magnesium (Mg), molybdenum (Mo), selenium (Se) and zinc (Zn) relative to LS cattle. There were reduced…
Advisors/Committee Members: Hendrick, Steve, McKinnon, John J., Penner, Greg B., Stookey, Joe M..
Subjects/Keywords: Dietary sulfur; beef cattle; thiamine and its phosphate esters; forage-to-concentrate ratio; short chain fatty acid absorption; mineral status
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Amat, S. (2012). Evaluation of sulfur and thiamine metabolism, short chain fatty acid absorption, and mineral status in beef cattle fed high dietary sulfur. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-12-838
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Amat, Samat. “Evaluation of sulfur and thiamine metabolism, short chain fatty acid absorption, and mineral status in beef cattle fed high dietary sulfur.” 2012. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2012-12-838.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Amat, Samat. “Evaluation of sulfur and thiamine metabolism, short chain fatty acid absorption, and mineral status in beef cattle fed high dietary sulfur.” 2012. Web. 23 Jan 2021.
Vancouver:
Amat S. Evaluation of sulfur and thiamine metabolism, short chain fatty acid absorption, and mineral status in beef cattle fed high dietary sulfur. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2012-12-838.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Amat S. Evaluation of sulfur and thiamine metabolism, short chain fatty acid absorption, and mineral status in beef cattle fed high dietary sulfur. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-12-838
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
4.
Girardin, Lynne.
Comparison of early (March) and late (June) calving systems on cow and pre-weaning calf performance and cost of production on western Canadian Prairies.
Degree: 2011, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2011-08-18
► A two-year study (2007, 2008) was conducted to evaluate the effects of two calving systems, early (March; Early Calving System (ECS)) vs. late (June; Late…
(more)
▼ A two-year study (2007, 2008) was conducted to evaluate the effects of two calving systems, early (March; Early Calving System (ECS)) vs. late (June; Late Calving System (LCS)) on cow, pre-weaning calf performance and feeding system management and costs. Both early and late calving systems were managed at three locations on the Canadian Prairies: Agriculture and Agri-food Canada (AAFC)-Brandon Research Centre (Brandon, Manitoba); AAFC-Semi arid Prairie Agricultural Research Centre (Swift Current, SK); and Western Beef Development Centre (Lanigan, SK). Four feeding management systems (drylot (DL), pasture (PG), swath-windrow (SG) and bale grazing (BG)) were utilized at all three locations to maximize grazing systems.
Management of animals through the four different feeding systems was found to meet or exceed protein and energy requirements according to NRC (2000). Differences in cow body weight (BW) (P=0.001; location) were observed across locations at pre-calving and weaning periods although there were no obvious patterns when comparing across calving systems. A significant three way interaction was observed for cow BW at breeding (P=0.003), and for cow body condition score (BCS) at breeding (P=0.002). Body condition score at breeding indicated there was a significant (P=0.002) three way interaction, where there were no significant interactions when comparisons across calving system within the same year (Y) and location (L) were performed for Brandon and Lanigan in 2007 and for Lanigan in 2008 also, therefore no improvements in one calving system compared to the other. At Brandon in 2007, ECS cow BCS were similar to LCS cows. In 2007 and 2008, the inverse occurred at SC where LCS cows had greater BCS compared to the ECS. The same two way (Calving System x Location) interaction was significant at pre-calving and weaning for both cow rib (P=0.003; P=0.007) and rump fat (P=0.002; P=0.02) where Lanigan had significantly lower rib and rump fat for the LCS as compared to the ECS. Rib and rump fat measurements did not follow a typical pattern. Fluctuations in body fat reserves varied depending on the calving system and location. Even though differences (P<0.05) occurred in cow BW and fat reserves, there was no significant difference (P>0.05) in reproductive performance between the two calving systems within the management of the current study. Pregnancy rate, calving rate, calving span and weaning rate were similar for both early and late calving systems.
In 2007, calf mortality on average was higher for LCS (5%) vs ECS (1.7%) and the inverse occurred in 2008, where LCS had lower calf mortalities than did ECS, 3.3% and 4%, respectively. Most calf mortalities were born dead or weak. There appeared to be no negative impact on calf mortality with early or late calving systems. A significant two way (Calving System x Year) interaction was observed for calf BW at birth (P=0.002) (Table 4.4). Treatment (Calving System) (P<0.0001) main effect was significant for ADG (Table 4.4). The average values for calf birth weights for…
Advisors/Committee Members: Lardner, Herbert A., Iwaasa, Alan D., McKinnon, John J., Hendrick, Steve, Schmutz, Sheila, Waldner, Cheryl.
Subjects/Keywords: calving; beef cow; calf performance; cow performance; calving season
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Girardin, L. (2011). Comparison of early (March) and late (June) calving systems on cow and pre-weaning calf performance and cost of production on western Canadian Prairies. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2011-08-18
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Girardin, Lynne. “Comparison of early (March) and late (June) calving systems on cow and pre-weaning calf performance and cost of production on western Canadian Prairies.” 2011. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2011-08-18.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Girardin, Lynne. “Comparison of early (March) and late (June) calving systems on cow and pre-weaning calf performance and cost of production on western Canadian Prairies.” 2011. Web. 23 Jan 2021.
Vancouver:
Girardin L. Comparison of early (March) and late (June) calving systems on cow and pre-weaning calf performance and cost of production on western Canadian Prairies. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2011-08-18.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Girardin L. Comparison of early (March) and late (June) calving systems on cow and pre-weaning calf performance and cost of production on western Canadian Prairies. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/ETD-2011-08-18
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
5.
Burgess, Brandy Ann.
The Development and Assessment of a Lung Biopsy Technique for Early BRD Detection.
Degree: 2009, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-07212009-100638
► The objectives of this project were: 1) to determine if live animal lung biopsy could be used to characterize early pathologic changes in the bovine…
(more)
▼ The objectives of this project were: 1) to determine if live animal lung biopsy could be used to characterize early pathologic changes in the bovine lung associated with bovine respiratory disease (BRD), 2) determine if specific infectious respiratory pathogens can be identified in association with early pathological changes, and 3) determine whether pulmonary pathology characterized by live animal lung biopsy at arrival and at the time of initial BRD diagnosis was associated with health and production outcomes of feedlot steers in a commercial feedlot.
A live animal percutaneous lung biopsy technique was developed to obtain a lung sample from the right middle lung lobe in intercostal space (ICS) 4 using a Bard® Magnum® reusable biopsy instrument and a modified 4-mm (8g) biopsy needle. The lung biopsy procedure was limited to 2 attempts per biopsy time. In the technique development, 34 animals chronically affected with BRD were utilized, 20 animals in the preliminary development followed by 14 additional animals in a commercial feedlot setting. The technique resulted in 1 fatality of 34 steers (2.9%) and lung parenchyma was harvested in 19 of 34 steers (55.9%) chronically affected with BRD. In addition, in the commercial feedlot setting this procedure was determined to take about 20 minutes per animal.
The final study was performed on one hundred feedlot steers considered at high risk of developing BRD from twenty pens within a commercial feedlot. Study animals were enrolled in three different groups: sick on arrival (ARR-SA) consisting of 27 study animals and 13 matched control animals; pen pulls with no fever (PP-NF) consisting of 14 study animals and matched 7 controls; and pen pulls with an undifferentiated fever (PP-UF) consisting of 26 study animals and 13 matched controls. Live animal percutaneous lung biopsies were collected from the right middle lung lobe at 3 different times within the first 30 days of the feeding period, about 2 weeks apart. All samples were histopathologically evaluated and were assessed for the presence of Mycoplasma bovis, Mannheimia haemolytica, Histophilus somni and bovine viral diarrhea virus with immunohistochemistry.
A total of 295 lung biopsies were performed yielding 210 (71.2%) lung samples that were sufficient for histopathological evaluation. A histopathology score was awarded to each biopsy based on certain histopathological lesions being present. Only 20 lung biopsy samples from 19 animals received a histopathology score (ie, pulmonary lesions were present) with the most common score being a 1 (maximum score is 20). There were too few lung biopsy samples with a histopathology score to reveal any association with subsequent health events.
Immunohistochemistry (IHC) was performed on all lung biopsies recovered yielding one lung sample to be positive for both Mannheimia haemolytica and Mycoplasma bovis from the PP-UF group. There were too few positive samples to reveal any association between IHC and histopathology score.
A post mortem evaluation was…
Advisors/Committee Members: Hendrick, Steve.
Subjects/Keywords: BRD; bovine respiratory disease; lung biopsy
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Burgess, B. A. (2009). The Development and Assessment of a Lung Biopsy Technique for Early BRD Detection. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-07212009-100638
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Burgess, Brandy Ann. “The Development and Assessment of a Lung Biopsy Technique for Early BRD Detection.” 2009. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/etd-07212009-100638.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Burgess, Brandy Ann. “The Development and Assessment of a Lung Biopsy Technique for Early BRD Detection.” 2009. Web. 23 Jan 2021.
Vancouver:
Burgess BA. The Development and Assessment of a Lung Biopsy Technique for Early BRD Detection. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/etd-07212009-100638.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Burgess BA. The Development and Assessment of a Lung Biopsy Technique for Early BRD Detection. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-07212009-100638
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
6.
Schurmann, Brittney.
FUNCTIONAL ADAPTATION OF THE RUMINAL EPITHELIUM.
Degree: 2013, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2013-12-1381
► Short chain fatty acids (SCFA) synthesized in the rumen from carbohydrate fermentation are an essential energy source for ruminants. Current literature supports that SCFA are…
(more)
▼ Short chain fatty acids (SCFA) synthesized in the rumen from carbohydrate fermentation are an essential energy source for ruminants. Current literature supports that SCFA are absorbed across the rumen epithelium via passive diffusion or protein-mediated transport, however, the rate and degree to which these pathways adapt to a change in diet fermentability is unknown. Furthermore, Na+ flux is partially determined by SCFA absorption, and thus is a key indicator of functional changes in the rumen epithelium. The objectives of this study were to determine the time required for a change in SCFA and Na+ absorption across the bovine rumen epithelium and to evaluate the rate and degree to which absorption pathways adapt to an increase in diet fermentability relative to changes in surface area. Twenty-five weaned Holstein steer calves were blocked by body weight and randomly assigned to either the control diet (CON; 91.5% hay and 8.5% vitamin/mineral supplement) or a moderately fermentable diet (50% hay; 41.5% barley grain, and 8.5% vitamin/mineral supplement) fed for 3 (G3), 7 (G7), 14 (G14), or 21 d (G21). All calves were fed at 2.25% BW at 0800 h. Reticular pH was recorded every 5 min for 48 h prior to killing (1000 h). Ruminal tissue was collected for Ussing chamber, barrier function, surface area measurements, and gene expression. Net 22Na+ flux (JNET-Na; 80 kBq/15 mL), the rate and pathway of mucosal to serosal 3H-acetate (JMS-acetate; 37 kBq/15 mL) and 14C-butyrate (JMS-butyrate; 74 kBq/15 mL) flux, and serosal to mucosal flux of 3H-mannitol (JSM-mannitol; 74 KBq/15 mL) and tissue conductance were measured. Half of the chambers assigned to measure JMS-acetate and JMS-butyrate were further assigned to 1 of 2 acetate and butyrate concentration treatments: 10 mM (Low) and 50 mM (High). Furthermore, JSM-mannitol flux was also measured during an acidotic and hyperosmotic challenge (CHAL) and recovery (REC) to measure barrier function of ruminal tissue. Mean reticular pH, which was positively correlated with ruminal pH (R2 = 0.5477), decreased from 6.90 for CON to 6.59 for G7 then increased. Net Na+ flux increased 125% within 7 d. Total JMS-acetate and JMS-butyrate increased from CON to G21, where passive diffusion was the primary SCFA absorption pathway. Total JMS-acetate and JMS-butyrate were greater when incubated in High vs. Low. Effective surface area of the ruminal epithelium was not affected by dietary treatment. Increased JSM-mannitol, tissue conductance, and increased expression of IL-1β and TLR2 (tendencies) with increased days fed the moderate grain diet indicated reduced rumen epithelium barrier function. Furthermore, the CHAL treatment reduced barrier function, which was not reversible during REC. This study indicates that a moderate increase in diet fermentability increases rumen epithelium absorptive function in the absence of increased SA, but reduces barrier function. Data from this study also suggests that absorption and barrier function follow different timelines, posing a challenge for ruminant diet…
Advisors/Committee Members: Penner, Gregory B., Christensen, David, McKinnon, John, Hendrick, Steve.
Subjects/Keywords: rumen epithelium; dietary adaptation; short chain fatty acid; ion transport; Ussing chamber
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Schurmann, B. (2013). FUNCTIONAL ADAPTATION OF THE RUMINAL EPITHELIUM. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-12-1381
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Schurmann, Brittney. “FUNCTIONAL ADAPTATION OF THE RUMINAL EPITHELIUM.” 2013. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2013-12-1381.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Schurmann, Brittney. “FUNCTIONAL ADAPTATION OF THE RUMINAL EPITHELIUM.” 2013. Web. 23 Jan 2021.
Vancouver:
Schurmann B. FUNCTIONAL ADAPTATION OF THE RUMINAL EPITHELIUM. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2013-12-1381.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Schurmann B. FUNCTIONAL ADAPTATION OF THE RUMINAL EPITHELIUM. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-12-1381
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
7.
Krause, Ashley.
EFFECTS OF UTILIZING CROP RESIDUES IN WINTER FEEDING SYSTEMS ON BEEF COW PERFORMANCE, REPRODUCTIVE EFFICIENCY AND ECONOMICS.
Degree: 2013, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2013-06-1119
► Over 2 years (Year 1, 2009-2010; Year 2, 2010-2011), two separate experiments were conducted to evaluate the effects of winter feeding system (n=3) on beef…
(more)
▼ Over 2 years (Year 1, 2009-2010; Year 2, 2010-2011), two separate experiments were conducted to evaluate the effects of winter feeding system (n=3) on beef cow performance, reproductive performance, economics and forage degradability. The three systems (treatments) were grazing pea crop residue (PEA) cv. ‘Performance 40-10’ (Year 1, TDN = 50.2%, CP = 7.3%; Year 2, TDN = 56.9%, CP = 8.9%) in field paddocks, grazing oat crop residue (OAT) cv. ‘Baler’ (Year 1, TDN = 59.1%, CP = 2.9%; Year 2, TDN = 66.9%, CP = 5.3%) in field paddocks, and feeding mixed grass-legume hay in drylot pens (DL) (Year 1, TDN = 61.4%; CP = 8.8%; Year 2, TDN = 52.3%, CP = 12.3%). In the first experiment, 90 dry, pregnant Black Angus cows (Year 1, 629 kg ± 74 kg; Year 2, 665 ± 69 kg) stratified by body weight (BW) and days pregnant were randomly allocated to 1 of the 3 systems. Cows were allocated feed in the field or pen on a 3 d basis and supplemented oat grain daily at 0.4-0.6% BW depending on environmental conditions. Dry matter intake (DMI) was estimated for each system using the herbage weight disappearance method. Cow BW, body condition score (BCS), and rib and rump fat were measured at start and end of trial and cow BW was corrected for conceptus gain based on calving data.
When data from the first 20 d were pooled over 2 years, initial cow BW was greater (P < 0.01) for the DL and OAT cows compared to the PEA cows and final cow BW was different (P < 0.01) between all 3 winter feeding systems. The change in BW was also greater (P < 0.01) for DL cows compared to cows on the OAT and PEA treatments. Analysis of the first 20 d of Year 1 study period and the total Year 2 study period, showed a significant (P < 0.01) year by treatment interaction for final BW and BW change. The differences (P < 0.01) in initial BW, final BW and BW change between the first 20 d of Year 1 study period and the total Year 2 study period (20 d) suggest feed quality, animal preference and weather conditions may cause difficulties when grazing residues in winter grazing systems.
Analysis of the entire trial period in Year 1 (62 d) indicates differences (P < 0.01) for final BW and BW change between cows on all three systems. The change in rib and rump fat was also different (P < 0.01) between cows in all 3 systems. In Year 2 (20 d), initial BW, final BW and BW change were different (P < 0.01) between DL and PEA cows, and between (P < 0.01) OAT and PEA cows. No difference (P > 0.05) was found for cow rib and rump fat in Year 2 and no difference (P > 0.05) was found for BCS in either Year 1 or Year 2 for cows managed in all 3 systems. Differences (P < 0.05) were observed for calving rate and calf birth weight between the DL and OAT system cows, but not between (P > 0.05) cows managed in the DL and PEA or OAT and PEA systems. Costs per cow per day were 1.22, 1.01 and 2.77 for PEA, OAT and DL systems in Year 1, respectively. In Year 2, cow costs per day were 1.59, 1.44 and 1.84 for PEA, OAT and DL systems, respectively.
In experiment 2, three ruminally cannulated,…
Advisors/Committee Members: Lardner, H. A., McKinnon, John J., Hendrick, Steve.
Subjects/Keywords: beef cow; crop residue; reproductive efficiency; system cost
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Krause, A. (2013). EFFECTS OF UTILIZING CROP RESIDUES IN WINTER FEEDING SYSTEMS ON BEEF COW PERFORMANCE, REPRODUCTIVE EFFICIENCY AND ECONOMICS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-06-1119
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Krause, Ashley. “EFFECTS OF UTILIZING CROP RESIDUES IN WINTER FEEDING SYSTEMS ON BEEF COW PERFORMANCE, REPRODUCTIVE EFFICIENCY AND ECONOMICS.” 2013. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2013-06-1119.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Krause, Ashley. “EFFECTS OF UTILIZING CROP RESIDUES IN WINTER FEEDING SYSTEMS ON BEEF COW PERFORMANCE, REPRODUCTIVE EFFICIENCY AND ECONOMICS.” 2013. Web. 23 Jan 2021.
Vancouver:
Krause A. EFFECTS OF UTILIZING CROP RESIDUES IN WINTER FEEDING SYSTEMS ON BEEF COW PERFORMANCE, REPRODUCTIVE EFFICIENCY AND ECONOMICS. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2013-06-1119.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Krause A. EFFECTS OF UTILIZING CROP RESIDUES IN WINTER FEEDING SYSTEMS ON BEEF COW PERFORMANCE, REPRODUCTIVE EFFICIENCY AND ECONOMICS. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-06-1119
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
8.
Walter, Lee-Anne Judy.
Comparison of wheat or corn dried distillers grains with solubles on performance, carcass characteristics, rumen fermentation parameters and diet digestibility of feedlot cattle.
Degree: 2010, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-01292010-172749
► Two trials were conducted, one using crossbred steers (Trial 1; N=275; 376±24 kg) and a second using Hereford heifers (Trial 2; N=5; 420±6 kg) to…
(more)
▼ Two trials were conducted, one using crossbred steers (Trial 1; N=275; 376±24 kg) and a second using Hereford heifers (Trial 2; N=5; 420±6 kg) to evaluate the performance, carcass quality, rumen fermentation and nutrient digestibility of cattle fed wheat or corn dried distillers grains with solubles (DDGS). The control ration averaged 87.7% rolled barley grain, 5.6% supplement and 6.8% barley silage (DM basis) over both trials. Dietary treatments included replacement of barley grain at 20 or 40% of the diet (DM basis) with wheat or corn DDGS. For Trial 1, steers were slaughtered at a constant finish weight of 645 kg. Data was analyzed as a completely randomized design using pen as the experimental unit. Feeding increasing levels of wheat DDGS quadratically increased dry matter intake (DMI) (P0.05). Feeding both wheat and corn DDGS increased (P=0.01) the excretion of nitrogen and phosphorus.
Replacement of barley grain with up to 40% corn or wheat DDGS improved gain:feed and reduced days on feed, respectively with no detrimental effect on carcass quality grade or sub-primal boneless boxed beef yield. The results of this project also indicate that the inclusion of corn and wheat DDGS (up to 40%) in feedlot rations does not mitigate ruminal acidosis, however the inclusion of both byproducts strongly impacts nutrient (crude fat, ADF, NDF, ADIN and NDIN) digestibility.
Advisors/Committee Members: McKinnon, John, Yu, Peiqiang, Hendrick, Steve, McAllister, Tim, Mutsvangwa, Tim.
Subjects/Keywords: wheat; cattle; DDGS; corn; feedlot performance; carcass quality
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Walter, L. J. (2010). Comparison of wheat or corn dried distillers grains with solubles on performance, carcass characteristics, rumen fermentation parameters and diet digestibility of feedlot cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-01292010-172749
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Walter, Lee-Anne Judy. “Comparison of wheat or corn dried distillers grains with solubles on performance, carcass characteristics, rumen fermentation parameters and diet digestibility of feedlot cattle.” 2010. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/etd-01292010-172749.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Walter, Lee-Anne Judy. “Comparison of wheat or corn dried distillers grains with solubles on performance, carcass characteristics, rumen fermentation parameters and diet digestibility of feedlot cattle.” 2010. Web. 23 Jan 2021.
Vancouver:
Walter LJ. Comparison of wheat or corn dried distillers grains with solubles on performance, carcass characteristics, rumen fermentation parameters and diet digestibility of feedlot cattle. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/etd-01292010-172749.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Walter LJ. Comparison of wheat or corn dried distillers grains with solubles on performance, carcass characteristics, rumen fermentation parameters and diet digestibility of feedlot cattle. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-01292010-172749
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
Klima, Cassidy.
Using comparative genomics to identify virulence traits and vaccine candidates in Mannheimia haemolytica.
Degree: 2015, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2015-06-2118
► Bovine respiratory disease (BRD) is the principal cause of morbidity and mortality among feedlot cattle. Mannheimia haemolytica is consistently implicated in this condition, but treatment…
(more)
▼ Bovine respiratory disease (BRD) is the principal cause of morbidity and mortality among feedlot cattle. Mannheimia haemolytica is consistently implicated in this condition, but treatment options are diminishing with the rise of antimicrobial resistance and intensifying consumer pressure to reduce reliance on conventional therapies. Thus, sustainable alternatives like vaccination are required. In this study, the phenotypic and genotypic diversity of BRD pathogens were examined with the objective to identify vaccine targets using reverse vaccinology, an innovative approach to identify antigens via genomic sequence. Preliminary surveillance confirmed M. haemolytica serotype 2 isolates were predominant in healthy animals (75.5%) while serotypes 1 (70.7%) and 6 (19.5%) were common in diseased animals. Pathogens of BRD, including M. haemolytica, Pasteurella multocida and Histophilus somni were also isolated from North American BRD mortalities, and compared using pulsed-field gel electrophoresis and antimicrobial susceptibility. Concurrently, polymerase chain reaction detection of bacterial and viral agents confirmed that M. haemolytica with bovine viral diarrhea virus were the most prevalent. Whereas isolates from live cattle were found to have a relatively low level of resistance, several pathogens from the mortalities were found to contain integrative conjugative elements (ICE) conferring resistance to seven antimicrobial classes. These ICEs were transferred via conjugation to other bacterial species, emphasizing the need for alternative antimicrobial therapies. Collectively, data from these investigations informed the selection of 11 diverse M. haemolytica strains for whole genome sequencing and comparative analyses. Several bacteriophage associated genes and CRISPR-Cas regulated gene expression systems were identified and are likely contributing to virulence in M. haemolytica. Coding sequences across all genomes were screened using pan-genome analysis, identifying 291 candidates with cell-surface associated signatures. Using a cell-free translation system and enzyme-linked immunosorbent assay the candidates were screened against serum from cattle challenged with serovar 1, 2 or 6 of M. haemolytica, and ranked according to immunogenicity. The top five vaccine candidates included Ssa1, ComE, a solute binding protein, an outer membrane protein, and the periplasmic component of an ABC transporter. With further characterization, these unique antigenic candidates could be developed into a vaccine to effectively reduce the dependence on antimicrobial therapies.
Advisors/Committee Members: McAllister, Tim, Hendrick, Steve, Potter, Andrew, Alexander, Trevor, Penner, Gregory.
Subjects/Keywords: bovine respiratory disease; antimicrobial resistance, reverse vaccinology, integrative conjugative elements, Mannheimia haemolytica, feedlot cattle
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Klima, C. (2015). Using comparative genomics to identify virulence traits and vaccine candidates in Mannheimia haemolytica. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-06-2118
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Klima, Cassidy. “Using comparative genomics to identify virulence traits and vaccine candidates in Mannheimia haemolytica.” 2015. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2015-06-2118.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Klima, Cassidy. “Using comparative genomics to identify virulence traits and vaccine candidates in Mannheimia haemolytica.” 2015. Web. 23 Jan 2021.
Vancouver:
Klima C. Using comparative genomics to identify virulence traits and vaccine candidates in Mannheimia haemolytica. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2015-06-2118.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Klima C. Using comparative genomics to identify virulence traits and vaccine candidates in Mannheimia haemolytica. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-06-2118
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
10.
Krone, Kristin.
EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS.
Degree: 2014, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2014-06-1580
► Previously, ADH1Cc.-64T>C was shown to have an association with intramuscular fat (IMF) in the longissimus thoracis (LT) muscle when vitamin A was limited in finishing…
(more)
▼ Previously, ADH1Cc.-64T>C was shown to have an association with intramuscular fat (IMF) in the longissimus thoracis (LT) muscle when vitamin A was limited in finishing rations of beef steers. The purpose of the current study was to determine the optimum vitamin A supplementation level, in combination with ADH1C genotype, to increase IMF of the LT muscle. Forty-five TT, 45 CT and 27 CC cross-bred steers, black in colour, were backgrounded on a commercial ration containing 3360 IU vitamin A/kg DM. During finishing the steers were randomly assigned to one of three vitamin A treatments at 25, 50 and 75% of the NRC recommendation of 2200 IU/kg DM. Treatments were administered via an oral bolus. Carcass quality was evaluated and a sample from the LT muscle was collected for analysis of IMF. A treatment x genotype interaction (P=0.04) was observed for IMF; TT steers on the 75% treatment had higher IMF relative to CT and CC steers on the same treatment. Intramuscular fat was also higher for TT steers on the 75% treatment in comparison to TT steers on the 25% treatment. Eighty-four percent of the steers graded Canada AAA. Western blot analysis showed that TT steers had higher (P=0.02) ADH1C levels in hepatic tissue. Previously, TT steers had increased IMF when fed limited vitamin A. In the current study the lack of variation between treatments and genotypes at the lower vitamin A treatment levels was likely due to the majority of the steers grading Canada AAA (USDA Choice). However, the western blot data supports that TT steers are expected to have higher IMF deposition, due to an increase production of ADH1C.
Advisors/Committee Members: Buchanan, Fiona C., McKinnon, John J., Hendrick, Steve, Hogan, Natacha.
Subjects/Keywords: Vitamin A; beef cattle; nutrigenetics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Krone, K. (2014). EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-06-1580
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Krone, Kristin. “EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS.” 2014. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2014-06-1580.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Krone, Kristin. “EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS.” 2014. Web. 23 Jan 2021.
Vancouver:
Krone K. EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2014-06-1580.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Krone K. EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-06-1580
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
11.
Van De Weyer, Leanne Margaret.
Serum micronutrient concentrations in western Canadian beef cattle at pre-breeding and pregnancy testing.
Degree: 2011, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-08122011-135644
► This thesis described the results of two studies that measured the concentrations of serum micronutrients in western Canadian beef cows at key production points, and…
(more)
▼ This thesis described the results of two studies that measured the concentrations of serum micronutrients in western Canadian beef cows at key production points, and examined associations between reproductive outcomes and micronutrient concentrations. The first study reported the serum copper and molybdenum concentrations at pregnancy testing time in cows from 66 cow-calf herds that were enrolled in a study of factors affecting productivity in 205 herds from western Canada. A relatively small proportion of cows had serum copper and molybdenum concentrations outside of adequate levels (16.2% of cows had below adequate serum copper, < 0.60 ppm; 12% of cows had high serum molybdenum, > 0.10 ppm). There were no associations between copper and molybdenum concentrations measured at the end of the grazing season and reproductive outcomes measured in these cows, with the exception that cows with the lowest serum copper concentrations at pregnancy testing were more likely to be pregnant than cows with higher copper concentrations. The practice of comparing serum copper and molybdenum concentrations between pregnant and non-pregnant animals in the fall as a tool in investigating poor pregnancy rates was not supported by this study.
In the second study, serum micronutrient concentrations of beef cows (n = 791) in southern
Saskatchewan were measured before placement onto summer grazing and breeding pastures and again at the end of the grazing season. Pre-breeding serum copper concentrations were less than adequate (< 0.60 ppm) in 75% of cows. High concentrations of serum molybdenum („d 0.10 ppm) were present in 19% of cows at pre-breeding. Cows < 10 years of age with lower pre-breeding serum copper concentrations were at increased odds of nonpregnancy. The greatest effect on pregnancy rates was observed for pre-breeding serum copper concentrations < 0.4 ppm. Season of measurement influenced the concentrations of serum micronutrients in these cows. Copper and vitamins A and E were higher in the fall, and molybdenum and selenium concentrations were lower in the fall.
These studies described serum micronutrient concentrations from healthy cows in western Canada at two production points, pre-breeding placement onto grazing pastures and pregnancy testing when cows are removed from grazing pasture. Identifying increased odds of nonpregnancy in cows with below adequate serum copper at pre-breeding emphasizes the importance of ensuring adequate copper concentrations in breeding females during this critical production phase.
Advisors/Committee Members: Waldner, Cheryl, Hendrick, Steve, Rosengren, Leigh, Stookey, Joe, Hill, Janet, Lardner, Bart.
Subjects/Keywords: reproduction; vitamins; trace minerals; epidemiology; nutrition; beef cattle
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Van De Weyer, L. M. (2011). Serum micronutrient concentrations in western Canadian beef cattle at pre-breeding and pregnancy testing. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08122011-135644
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Van De Weyer, Leanne Margaret. “Serum micronutrient concentrations in western Canadian beef cattle at pre-breeding and pregnancy testing.” 2011. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/etd-08122011-135644.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Van De Weyer, Leanne Margaret. “Serum micronutrient concentrations in western Canadian beef cattle at pre-breeding and pregnancy testing.” 2011. Web. 23 Jan 2021.
Vancouver:
Van De Weyer LM. Serum micronutrient concentrations in western Canadian beef cattle at pre-breeding and pregnancy testing. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/etd-08122011-135644.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Van De Weyer LM. Serum micronutrient concentrations in western Canadian beef cattle at pre-breeding and pregnancy testing. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/etd-08122011-135644
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
12.
Garcia Guerra, Alvaro.
Improving diagnostic techniques for venereal diseases in bulls.
Degree: 2013, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2013-06-1085
► Infectious disease continues to cause significant problems on reproductive efficiency in the cattle industry. The purpose of this project is to evaluate new testing strategies…
(more)
▼ Infectious disease continues to cause significant problems on reproductive efficiency in the cattle industry. The purpose of this project is to evaluate new testing strategies for Tritrichomonas foetus and Campylobacter fetus subsp. venerealis.
This thesis describes the result of three studies that evaluated the use of real-time PCR for the identification of Tritrichomonas foetus and Campylobacter fetus subsp. venerealis in carrier bulls. The first study evaluated the specificity of a real-time PCR test for T. foetus in individual culture enriched samples, and the sensitivity of the assay for use in pooled samples of up to 25 bulls. Specificity estimates were 98.8% (95% CI 97-99.4) and 100% (95% CI 98.9-100) for culture and real-time PCR, respectively. The sensitivity of the real-time PCR assay for pooled preputial samples was: 96.8% (83.8-99.4) for pool ratios 1/3 and 1/5; 93.5% (79.3-98.2) for pool ratios 1/2, 1/15, 1/20 and 1/25; and 90.3% (75.1-96.6), and were not significantly different. However, 13 of the 217 pools tested were negative and 9 of these negative testing pools contained the same positive sample. The media in this positive sample showed evidence of contamination and could potentially explain the failure to detect T. foetus.
The second study evaluated the sensitivity of a real-time PCR for the detection of T. foetus in individual and pooled direct preputial samples. Sensitivity of individual samples tested by culture, real-time PCR in direct and culture enriched samples were determined from 121 samples obtained from 9 infected bulls. Sensitivity estimates were: 95.0% (95% CI: 89.6% to 97.7%) for culture, 95.9% (95% CI: 90.7 to 98.2) for real-time PCR in cultured enriched samples, and 90.1% (95% CI: 83.5 to 94.2) for direct preputial samples and did not differ (P=0.12). Sensitivity estimates for direct pooled samples in groups of 5 or 10 were: 83.6% (95% CI: 75.6 to 89.4) and 77.3% (95% CI: 68.6-84.1), respectively and were not significantly different (P=0.08). The use of repeat sampling tested in pools by real-time PCR increased the sensitivity to 100% and 96% for 3 consecutive samples (pools of 5 or 10, respectively). The use of pooled direct preputial samples although sensitive, still requires the use of repeated sampling.
The third study determined the sensitivity and specificity of a recently developed real-time PCR (qPCR) tests for Cfv. A total of 300 virgin bulls were tested by both culture and qPCR. Specificity estimates were 85% (95% CI: 80.5 to 88.6) for qPCR and 100% (95% CI: 98.7 to 100) for culture, and were significantly different (P<0.01). A total of 4 naturally infected bulls and 9 artificially infected bulls were sampled serially to obtain positive samples for a sensitivity analysis. Sensitivity estimates and 95% confidence intervals are as follows: qPCR (85.4%, 95% CI: 80.6-89.2); direct culture on blood agar (82.3%, 95% CI: 77.2-86.5), DFAT (72.1%, 95% CI: 66.2-77.4), direct culture on Skirrow agar (32.7%, 95% CI: 27.2-38.7), TEM and blood agar (30%, 95% CI: 23.4-37.5), and TEM…
Advisors/Committee Members: Hendrick, Steve H., Barth, Albert D., Waldner, Cheryl, Hill, Janet E., Joseph, Stookey.
Subjects/Keywords: Campylobacter fetus subspecies venerealis; Tritrichomonas foetus, PCR
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Garcia Guerra, A. (2013). Improving diagnostic techniques for venereal diseases in bulls. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-06-1085
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Garcia Guerra, Alvaro. “Improving diagnostic techniques for venereal diseases in bulls.” 2013. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2013-06-1085.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Garcia Guerra, Alvaro. “Improving diagnostic techniques for venereal diseases in bulls.” 2013. Web. 23 Jan 2021.
Vancouver:
Garcia Guerra A. Improving diagnostic techniques for venereal diseases in bulls. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2013-06-1085.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Garcia Guerra A. Improving diagnostic techniques for venereal diseases in bulls. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-06-1085
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
13.
Petri, Renee.
IMPACT OF DIET COMPOSITION ON RUMEN BACTERIAL PHYLOGENETICS.
Degree: 2013, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2013-02-931
► ABSTRACT Two experiments were conducted to determine the effects of various forage to concentrate ratios on the rumen microbial ecosystem and rumen fermentation parameters using…
(more)
▼ ABSTRACT
Two experiments were conducted to determine the effects of various forage to concentrate ratios on the rumen microbial ecosystem and rumen fermentation parameters using culture-independent methods. In the first experiment, cattle were fed either a high concentrate (HC) or a high concentrate without forage (HCNF) diet. Comparison of rumen fermentation parameters between these two diets showed that duration of time spent below pH 5.2 and rumen osmolality were higher for HCNF. Calculations using Simpson’s index showed a greater diversity of dominant species for HCNF than in HC based on 16S rRNA PCR-DGGE. Real-time real-time PCR showed populations of Fibrobacter succinogenes (P=0.01) were lower in HCNF than HC diets. Ruminococcus spp., F. succinogenes and Selenomonas ruminantium were present at higher (P≤0.05) concentrations in solid than in liquid digesta in both diets. The second experiment compared cattle as they adapted from a strictly forage to a concentrate diet, after which they were subject to an acidotic challenge and a recovery period (Forage, Mixed Forage, High Grain, Acidosis and Recovery). A total of 153,621 high-quality bacterial sequences were obtained from biopsied rumen epithelium, and 407,373 sequences from the solid and liquid phases of rumen contents. Only 14 epithelial genera representing >1.0% of the epimural population differed (P ≤ 0.05) among dietary treatments. However, clustering showed a closer relation in bacterial profiles for the Forage and Mixed Forage diets as compared to the High Grain, Acidosis and Recovery diets. Several epithelial identified genera including Atopobium, Desulfocurvus, Fervidicola, Lactobacillus and Olsenella increased as a result of acidosis. However, any changes in bacterial populations during the acidosis challenge were not sustained during the recovery period. This indicates a high level of stability within the rumen epimural community. An epithelial core microbiome was determined which explained 21% of the enumerable rumen population across all treatment samples. Cluster analysis of the solid and liquid phase rumen bacterial showed that these populations differed (P ≤ 0.10) between forage and grain-based diets. Rumen core microbiome analysis found 32 OTU’s representing 10 distinct bacterial taxa in whole rumen contents for all dietary treatments. Heifers that developed clinical acidosis vs the subclinical acidosis showed increases in the genera Acetitomaculum, Lactobacillus, Prevotella, and Streptococcus. Variation in microbial taxa as an effect of both treatment and animal was evident in the solid and liquid fractions of the rumen digesta. However, impacts of a dietary treatment were transient and despite an acidotic challenge, rumen microbiota were able to recover within a week of perturbation. The bacterial populations in the rumen are highly diverse as indicated by DGGE analysis and showed clear distinction between not only dietary treatments, individual animals, but also between epithelial, liquid and solid associated populations on the same diet.…
Advisors/Committee Members: McAllister, Tim A., McKinnon, John J., Mutsvangwa, Tim, VanKessel, Andrew, Hendrick, Steve, Beauchemin, Karen.
Subjects/Keywords: real-time PCR; DGG; pyrosequencing; cattle; rumen
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Petri, R. (2013). IMPACT OF DIET COMPOSITION ON RUMEN BACTERIAL PHYLOGENETICS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-02-931
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Petri, Renee. “IMPACT OF DIET COMPOSITION ON RUMEN BACTERIAL PHYLOGENETICS.” 2013. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/ETD-2013-02-931.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Petri, Renee. “IMPACT OF DIET COMPOSITION ON RUMEN BACTERIAL PHYLOGENETICS.” 2013. Web. 23 Jan 2021.
Vancouver:
Petri R. IMPACT OF DIET COMPOSITION ON RUMEN BACTERIAL PHYLOGENETICS. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/ETD-2013-02-931.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Petri R. IMPACT OF DIET COMPOSITION ON RUMEN BACTERIAL PHYLOGENETICS. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-02-931
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
14.
Wiese, Brittany I 1988-.
DEFINING THE RISK, PREVALENCE, AND PATHOLOGICAL THRESOLD OF LOW RUMINAL pH IN FEELOT CATTLE.
Degree: 2017, University of Saskatchewan
URL: http://hdl.handle.net/10388/7770
► The diet transition phase is thought to be the highest risk period for development of low ruminal pH, while pathology associated with low reticulo-ruminal pH…
(more)
▼ The diet transition phase is thought to be the highest risk period for development of low ruminal pH, while pathology associated with low reticulo-ruminal pH (RRpH) induced ruminal acidosis (RA) is often found at slaughter, months after the diet transition. Two experiments were conducted to 1) determine the risk of low RRpH during the transition phase and 2) explore the association of rumen fermentation and acute phase protein response during finishing with pathology identified post mortem. In experiment 1, RRpH was measured in 32 mixed breed steers (n = 16) and heifers (n = 16) housed in commercial feedlot pens with 227 ±13 and 249 ± 6 hd/pen cohort steers and heifers, respectively. Cattle were transitioned from a diet containing 46.5% forage and 53.5% concentrate to a diet containing 9.5% forage and 90.5% concentrate dry matter (DM) basis) over 40 d. In addition, wheat replaced barley as the grain source during the dietary transition. Both mean and minimum RRpH decreased as the proportion of concentrate in the diet increased. The area (duration severity) that RRpH was < 5.6, duration that RRpH was <5.6, and the number of cattle experiencing a bout of low RRpH (pH < 5.6 for > 180 min), increased with increasing concentrate. Despite having a high risk for low RRpH, most cattle had only 1-3 bouts of low RRpH during the diet transition, and extent was mild. Steers had greater dry matter intake (DMI), lower RRpH, and greater standard deviation of RRpH than heifers, suggesting that susceptibility to RA may differ between steers and heifers. In experiment 2, ruminal pH, short-chain fatty acid concentrations and serum acute phase proteins were measured in 28 cannulated steers during the final 5 wk of finishing when fed a diet containing 5% forage and 95% concentrate (DM basis). Rumen and livers were examined and pathology scores were determined at slaughter. There was no difference in minimum pH, mean pH, or duration that ruminal pH was < 5.5 between steers with or without pathology. However, steers with pathology spent more time with ruminal pH < 5.2 and tended to spend more time with ruminal pH < 5.8. Acetate concentration tended to be greater in steers with pathology than without pathology. Serum amyloid A was greater and haptoglobin tended to be greater in steers with pathology than those without. Overall, liver and rumen pathology was associated with a greater duration that ruminal pH is < 5.2 and a chronic systemic acute phase protein response. In summary, feedlot cattle experience low RRpH during dietary transition and that the risk increases with increasing levels of concentrate. However, during the dietary transition the extent of low RRpH was mild. During the last 5 wk of finishing, the duration that ruminal pH was < 5.2 and the plasma concentration of serum amyloid A, were associated with greater rumen and liver pathology scores, suggesting that low ruminal pH occurring during the latter part of finishing may have an impact on risk for rumenitis and liver abscesses.
Advisors/Committee Members: Penner, Greg B, Campbell, John G, Jelinski, Murray, Hendrick, Steve, McKinnon, John J, Timsit, Eduoard, Harding, John.
Subjects/Keywords: acidosis; ruminal pH; feedlot cattle; liver abscess; acute phase protein
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wiese, B. I. 1. (2017). DEFINING THE RISK, PREVALENCE, AND PATHOLOGICAL THRESOLD OF LOW RUMINAL pH IN FEELOT CATTLE. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7770
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wiese, Brittany I 1988-. “DEFINING THE RISK, PREVALENCE, AND PATHOLOGICAL THRESOLD OF LOW RUMINAL pH IN FEELOT CATTLE.” 2017. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/7770.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wiese, Brittany I 1988-. “DEFINING THE RISK, PREVALENCE, AND PATHOLOGICAL THRESOLD OF LOW RUMINAL pH IN FEELOT CATTLE.” 2017. Web. 23 Jan 2021.
Vancouver:
Wiese BI1. DEFINING THE RISK, PREVALENCE, AND PATHOLOGICAL THRESOLD OF LOW RUMINAL pH IN FEELOT CATTLE. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/7770.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wiese BI1. DEFINING THE RISK, PREVALENCE, AND PATHOLOGICAL THRESOLD OF LOW RUMINAL pH IN FEELOT CATTLE. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7770
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
15.
Douma, Dale Peter.
Investigation of the distribution and risk factors associated with Mycobacterium avium subspecies paratuberculosis in cow-calf herds in Canada.
Degree: 2010, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-03312011-114913
► This thesis summarizes an investigation of Mycobacterium avium subspecies paratuberculosis (Map) as a pathogen within the cow-calf industry in Canada. The specific objectives of this…
(more)
▼ This thesis summarizes an investigation of Mycobacterium avium subspecies paratuberculosis (Map) as a pathogen within the cow-calf industry in Canada. The specific objectives of this project were to describe the distribution of this pathogen in this industry provincially, as well as at the individual farm level in wildlife species, and in the environment. Secondary objectives of this project were to identify on-farm management risk factors that are associated with this disease and to examine potential options for herd level diagnostic capabilities. Nationally, 0.8% (95%CI = 0.4-1.1%) of the cows in the cow-calf industry were seropositive for Map with 11.7% (95%CI=7.0-16.5%) of the herds sampled having a minimum of one positive test result or 4.5% (95%CI=1.4-7.5%) of the herds having a minimum of two positive test results. The true cow prevalence was estimated as 1.8% (95%CI= 0.4 – 3.1). No Map was detected in any of the non-ruminant wildlife species sampled on cow-calf operations suggesting that these species were not of primary concern when dealing with the management of this disease. In a study not focussed on a cow-calf operation, Map was detected in one cluster of trapped coyote samples in a region with cow-calf production. The prevalence of Map infection in this cluster of coyotes was calculated to be 9.1% (CI: 5.7-12.5). The prevalence of infection in coyotes including all sites, ignoring the effect of clustering, was calculated to be 3.7% (CI: 2.3-5.1). The use of a commercial colostrum replacement on farm (Odds Ratio =3.96; 95% CI = 1.10–14.23, p=0.035) and the presence of wild deer interacting with the cattle (Odds Ratio = 14.32; 95% CI = 1.13–181.90, p=0.040) were positively associated with being a herd infected with paratuberculosis. The use of rotational grazing practices was protective (Odds Ratio = 0.20; 95% CI = 0.04–0.93, p=0.039). It was possible to detect environmental contamination with Map on cow-calf farms using bacterial culture and PCR for confirmation. No water samples were positive to Map; however, 6.2% of the non-water environmental samples were positive. The use of an environmental sampling protocol had a herd sensitivity of 29.6%. This finding led to a simulation modelling study to evaluate how various testing methods would compare in the broader population of cow-calf herds. The final mean risk of selecting a herd infected with Map that was not identified as positive via the herd screen test strategy was 12.9%, 9.8%, 9.6%, and 6.1% for no herd screen test, environmental sampling, ELISA serology, and pooled fecal culture strategies, respectively.
Advisors/Committee Members: Campbell, John, Stookey, Joseph, VanLeeuwen, John, Hendrick, Steve, Waldner, Cheryl, Wobeser, Gary.
Subjects/Keywords: Johne's; beef; environment; wildlife; management; risk analysis
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Douma, D. P. (2010). Investigation of the distribution and risk factors associated with Mycobacterium avium subspecies paratuberculosis in cow-calf herds in Canada. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-03312011-114913
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Douma, Dale Peter. “Investigation of the distribution and risk factors associated with Mycobacterium avium subspecies paratuberculosis in cow-calf herds in Canada.” 2010. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/etd-03312011-114913.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Douma, Dale Peter. “Investigation of the distribution and risk factors associated with Mycobacterium avium subspecies paratuberculosis in cow-calf herds in Canada.” 2010. Web. 23 Jan 2021.
Vancouver:
Douma DP. Investigation of the distribution and risk factors associated with Mycobacterium avium subspecies paratuberculosis in cow-calf herds in Canada. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/etd-03312011-114913.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Douma DP. Investigation of the distribution and risk factors associated with Mycobacterium avium subspecies paratuberculosis in cow-calf herds in Canada. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-03312011-114913
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
16.
Heendeniya Vidanaralalage, Ravindra Guptha.
Utilization of canola seed fractions in ruminant feeds.
Degree: 2008, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-06162008-141937
► Canola fibre-protein and can-sugar are the two by-products arising from a process for separating high quality protein fractions from canola meal. In the first trial…
(more)
▼ Canola fibre-protein and can-sugar are the two by-products arising from a process for separating high quality protein fractions from canola meal. In the first trial chemical characteristics of fibre-protein and can-sugar were examined in comparison with commercial canola and soy meal. In the second trial in situ rumen degradability and kinetics of test feed was studied. Based on the findings of those two trials, available energy values were estimated based on NRC (2001) while protein contents potentially absorbable at small intestine were predicted using both NRC (2001) and DVE/OEB models. Subsequently a mixture of fibre-protein and can-sugar was used as an additive to dehydrated alfalfa pellet and two dairy cow trials were conducted to determine the palatability and examine effect on lactation performances of blended alfalfa pellet feeding in comparison with standard alfalfa pellet. Palatability difference was evaluated by “Paterson -two choice alternating access method” through a 7 day experimental period using 6 lactating Holstein cows. In the lactating performance trial, 6 cows were randomly assigned into two groups and two treatments were allocated over three experimental periods in a switchback design. Can-sugar consisted of water soluble components (CP 15.6 %DM; SCP 96.2 %CP; NFC 99.9 %CHO) with non-protein nitrogen as the main CP fraction (NPN 96.2 %CP). Fibre-protein was a highly fibrous material (NDF: 55.6%; ADF: 46.3%; ADL: 24.1%) comparing to canola meal (NDF: 25.4%, ADF: 21.2%, ADL: 9.0%) due to presence of higher level of seed hulls in fibre-protein. Comparing to canola meal, fibre-protein contained 9% less CP and 1/4 of that consisted of undegradable ADIP. Rumen degradability of can-sugar was assumed as immediate and total as it was water soluble. Most of the ruminally undegradable nutrient components present in canola meal appeared to be concentrated into fibre protein during the manufacturing process and as a result fibre-protein has shown a consistently lower effective degradability of DM, OM, CP NDF and ADF comparing to both canola and soy meal. Available energy content in can-sugar was marginally higher than that of canola meal while fibre-protein contained only 2/3 that of canola meal. The predicted absorbable protein content at small intestine was about 1/2 that of canola meal. These results indicate that fibre-protein can be considered as a secondary source of protein in ruminant feed and a mixture of fibre-protein and can-sugar would nutritionally complement each other to formulate into a cheaper ingredient in ruminant ration. In the palatability study, there was no significant difference (P>0.05) in intake preference or finish time between the blended and standard alfalfa pellets. The results from the lactation study showed that there was no significant difference (P>0.05) in milk yield, dairy efficiency or milk composition between the blended and standard alfalfa pellets. The results from the two studies indicated that fibre-protein and can-sugar fractions could be used as an additive to…
Advisors/Committee Members: Yu, Peiqiang, McKinnon, John J., Maenz, David D., Hendrick, Steve, Christensen, David A., Buchanan, Fiona C..
Subjects/Keywords: energy availability; protein availability; DVE/OEB; palatability; can-sugar; fibre-protein; canola seed fractions; Canola; in situ charachteristics; Ruminant; canola protein fractionation; feed; chemical charachteristics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Heendeniya Vidanaralalage, R. G. (2008). Utilization of canola seed fractions in ruminant feeds. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06162008-141937
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Heendeniya Vidanaralalage, Ravindra Guptha. “Utilization of canola seed fractions in ruminant feeds.” 2008. Thesis, University of Saskatchewan. Accessed January 23, 2021.
http://hdl.handle.net/10388/etd-06162008-141937.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Heendeniya Vidanaralalage, Ravindra Guptha. “Utilization of canola seed fractions in ruminant feeds.” 2008. Web. 23 Jan 2021.
Vancouver:
Heendeniya Vidanaralalage RG. Utilization of canola seed fractions in ruminant feeds. [Internet] [Thesis]. University of Saskatchewan; 2008. [cited 2021 Jan 23].
Available from: http://hdl.handle.net/10388/etd-06162008-141937.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Heendeniya Vidanaralalage RG. Utilization of canola seed fractions in ruminant feeds. [Thesis]. University of Saskatchewan; 2008. Available from: http://hdl.handle.net/10388/etd-06162008-141937
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
.