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University of Saskatchewan
1.
Haile, Teketel A.
Genomic Selection, Quantitative Trait Loci and Genome-Wide Association Mapping for Spring Bread Wheat (Triticum aestivum L.) Improvement.
Degree: 2018, University of Saskatchewan
URL: http://hdl.handle.net/10388/8437
► Molecular breeding involves the use of molecular markers to identify and characterize genes that control quantitative traits. Two of the most commonly used methods to…
(more)
▼ Molecular breeding involves the use of molecular markers to identify and characterize genes that control quantitative traits. Two of the most commonly used methods to dissect complex traits in plants are linkage analysis and association mapping. These methods are used to identify markers associated with quantitative trait loci (QTL) that underlie trait variation, which are used for marker assisted selection (MAS). Marker assisted selection has been successful to improve traits controlled by moderate to large effect QTL; however, it has limited application for traits controlled by many QTL with small effects. Genomic selection (GS) is suggested to overcome the limitation of MAS and improve genetic gain of quantitative traits. GS is a type of MAS that estimates the effects of genome-wide markers to calculate genomic estimated breeding values (GEBVs) for individuals without phenotypic records. In recent years, GS is gaining momentum in crop breeding programs but there is limited empirical evidence for practical application. The objectives of this study were to: i) evaluate the performance of various statistical approaches and models to predict agronomic and end-use quality traits using empirical data in spring bread wheat, ii) determine the effects of training population (TP) size, marker density, and population structure on genomic prediction accuracy, iii) examine GS prediction accuracy when modelling genotype-by-environment interaction (G × E) using different approaches, iv) detect marker-trait associations for agronomic and end-use quality traits in spring bread wheat, v) evaluate the effects of TP composition, cross-validation technique, and genetic relationship between the TP and SC on GS accuracy, and vi) compare genomic and phenotypic prediction accuracy. Six studies were conducted to meet these objectives using two populations of 231 and 304 spring bread wheat lines that were genotyped with the wheat 90K SNP array and phenotyped for nine agronomic and end-use quality traits. The main finding across these studies is that GS can accurately predict GEBVs for wheat traits and can be used to make predictions in different environments; thus, GS should be applied in wheat breeding programs. Each study provides specific insights into some of the advantages and limitations of different GS approaches, and gives recommendations for the application of GS in future breeding programs. Specific recommendations include using the GS model BayesB (especially for large effect QTL) for genomic prediction in a single environment, across-year genomic prediction using the reaction norm model, using a large TP size for making accurate genomic predictions, and not making across-population genomic predictions except for highly related populations
Advisors/Committee Members: Pozniak, Curtis J, Bai, Yuguang, Hucl, Pierre J, Beattie, Aaron D, Buchanan, Fiona C.
Subjects/Keywords: Cross-validation; Genomic selection; Genomic estimated breeding value; Quantitative trait loci; Training population; Selection Candidates; Validation population
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APA ·
Chicago ·
MLA ·
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APA (6th Edition):
Haile, T. A. (2018). Genomic Selection, Quantitative Trait Loci and Genome-Wide Association Mapping for Spring Bread Wheat (Triticum aestivum L.) Improvement. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/8437
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Haile, Teketel A. “Genomic Selection, Quantitative Trait Loci and Genome-Wide Association Mapping for Spring Bread Wheat (Triticum aestivum L.) Improvement.” 2018. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/8437.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Haile, Teketel A. “Genomic Selection, Quantitative Trait Loci and Genome-Wide Association Mapping for Spring Bread Wheat (Triticum aestivum L.) Improvement.” 2018. Web. 11 Apr 2021.
Vancouver:
Haile TA. Genomic Selection, Quantitative Trait Loci and Genome-Wide Association Mapping for Spring Bread Wheat (Triticum aestivum L.) Improvement. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/8437.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Haile TA. Genomic Selection, Quantitative Trait Loci and Genome-Wide Association Mapping for Spring Bread Wheat (Triticum aestivum L.) Improvement. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/8437
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
2.
Leao Guidotti, Elisabeth 1982-.
ESTABLISHMENT OF CARINATA MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE.
Degree: 2018, University of Saskatchewan
URL: http://hdl.handle.net/10388/8648
► The objective of this research was to evaluate the value of carinata meal (CRM) relative to canola meal (CM) when fed alone or in combination…
(more)
▼ The objective of this research was to evaluate the value of carinata meal (CRM) relative to canola meal (CM) when fed alone or in combination with wheat-dried distillers grains with solubles (WDDGS) on the performance of growing and finishing beef steers and rumen fermentation, total tract nutrient utilization, omasal flow and N efficiency of growing beef heifers. The first trial involved a 97-d backgrounding (BK) trial that used 360 calves (321.8 ± 0.10kg) assigned to one of 12 pens. Diets compared CRM relative to CM at two dietary inclusion levels (7.5 and 15% DM basis). The second trial was a finishing trial using 250 crossbred steers (418.7 ± 0.48 kg) assigned to 25 pens with five treatments: CRM (4.8% DM), CM (6% DM), WDDGS (6.2% DM), and CRM (2.7% DM) + WDDGS (2.7% DM) or CM (3% DM) + WDDGS (3% DM). Trial three designed as a Latin square, used 4 rumen-cannulated heifers (385.8 ± 27.95 kg) that were fed a barley-based BK diet supplemented with CRM (9.24% DM); CM (9.97% DM); CRM (4.98% DM) + WDDGS (5.03% DM) or CM (4.98% DM) + WDDGS (5.03% DM). In Trial 1 and 2, there were no differences (P > 0.05) between treatments for final shrunk BW or ADG, DMI and G:F. In trial 2, cattle fed CM had heavier hot carcass weights and a greater dressing percentage (DP) than those fed CRM diets. In Trial 3, apparent digestion of N tended (P = 0.09) to be greater for CRM and CM diets relative to WDDGS diets. The inclusion of WDDGS increased (P = 0.04) N truly digested in the rumen, and decreased ruminal non- ammonia nitrogen (NAN) flow. No treatment differences (P > 0.05) were noted in total bacterial NAN flow or in microbial efficiency. Carinata meal is equal to CM as a protein source for beef cattle without affecting performance, rumen fermentation, total tract nutrient utilization, and N efficiency. However, HCW and DP were greater in cattle fed CM relative to those fed CRM. There was no benefit to adding WDDGS as a rumen undegradable protein source.
Advisors/Committee Members: McKinnon, John J, Penner , Gregory B, Christensen, David A, Yu, Peiqiang, Buchanan, Fiona C, Erickson, Nathan.
Subjects/Keywords: carinata meal; canola meal; wheat-dried distillers’grains with solubles; feedlot; performance; carcass characteristics; rumen fermentation; omasal flow; microbial protein synthesis; digestibility
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Leao Guidotti, E. 1. (2018). ESTABLISHMENT OF CARINATA MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/8648
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Leao Guidotti, Elisabeth 1982-. “ESTABLISHMENT OF CARINATA MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE.” 2018. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/8648.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Leao Guidotti, Elisabeth 1982-. “ESTABLISHMENT OF CARINATA MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE.” 2018. Web. 11 Apr 2021.
Vancouver:
Leao Guidotti E1. ESTABLISHMENT OF CARINATA MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/8648.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Leao Guidotti E1. ESTABLISHMENT OF CARINATA MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/8648
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
3.
Sutherland, Brittney.
Barley Silage or Corn Silage Fed in Combination with Barley Grain, Corn Grain, or a Blend of Barley and Corn Grain to Backgrounding Beef Cattle.
Degree: 2020, University of Saskatchewan
URL: http://hdl.handle.net/10388/12821
► The objective of this study was to determine the effect of either barley (BS) or corn (CS) silage fed with dry-rolled barley (BG), dry-rolled corn…
(more)
▼ The objective of this study was to determine the effect of either barley (BS) or corn (CS) silage fed with dry-rolled barley (BG), dry-rolled corn (CG), or a blend of barley and corn grain (BCG) on predicted nutrient digestibility and growth performance of backgrounding cattle (Study 1) and dry matter intake, ruminal fermentation, total tract digestibility and nitrogen balance (Study 2).In study 1, Steers (n = 288) were stratified by BW into 24 pens and pens were randomly assigned to one of six treatments (n = 4) in a two three factorial design. For Study 2 five ruminally cannulated heifers were used in an incomplete 6 6 Latin square design. Periods were 25-d including five days of dietary transition, 13 days of dietary adaptation, and seven days of sample collection. Treatments contained (DM basis) either BS or CS included at 55% in combination with 30% BG, CG, or BCG, 8% canola meal, varying amounts of urea to balance CP, and 5% of a mineral and vitamin supplement. There were no interactions among silage or grain source and no differences in ADG (1.01 kg/d) or G:F (0.10 kg/kg) among diets. However, DMI was 0.8 kg/d greater for steers fed CS (P = 0.018) than BS. Final BW was 8.4 kg greater for steers fed CS (P = 0.004) compared to steers fed BS. Fecal starch was greatest for CG, intermediate for BCG, and least for BG (P < 0.01). Whole barley kernels appearing in feces were greatest in BG compared to BCG while partial corn kernels in feces were greater in CG compared BCG (P < 0.01). Fine fibre particles in feces were greatest in BG diets with CG and BCG being least (P < 0.01 In study 2 Acetate concentrations were greatest for the CG and BCG diets (P < 0.01) while propionate was greatest for BS-BG and the least for CS-BCG (P < 0.05). Rumen ammonia concentrations were greatest for CG treatments (P < 0.01). Barley grain had greater DM, OM, starch and GE digestibility comparted to CG with BCG being intermediate (P < 0.05). Fecal nitrogen excretion was greatest for cattle fed CS (P < 0.05) as wells as for CG (P < 0.01). Use of CS improved DMI, ending BW and nutrient digestibility; while dry-rolled BG improved nutrient digestibility and reduced fecal starch concentration as compared to CG or BCG in diets for backgrounding cattle.
Advisors/Committee Members: Penner, Gregory B, McKinnon, John J, McAllister, Tim A, Caton, Joel, Buchanan, Fiona C.
Subjects/Keywords: short-season corn; starch
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Sutherland, B. (2020). Barley Silage or Corn Silage Fed in Combination with Barley Grain, Corn Grain, or a Blend of Barley and Corn Grain to Backgrounding Beef Cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12821
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sutherland, Brittney. “Barley Silage or Corn Silage Fed in Combination with Barley Grain, Corn Grain, or a Blend of Barley and Corn Grain to Backgrounding Beef Cattle.” 2020. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/12821.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sutherland, Brittney. “Barley Silage or Corn Silage Fed in Combination with Barley Grain, Corn Grain, or a Blend of Barley and Corn Grain to Backgrounding Beef Cattle.” 2020. Web. 11 Apr 2021.
Vancouver:
Sutherland B. Barley Silage or Corn Silage Fed in Combination with Barley Grain, Corn Grain, or a Blend of Barley and Corn Grain to Backgrounding Beef Cattle. [Internet] [Thesis]. University of Saskatchewan; 2020. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/12821.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sutherland B. Barley Silage or Corn Silage Fed in Combination with Barley Grain, Corn Grain, or a Blend of Barley and Corn Grain to Backgrounding Beef Cattle. [Thesis]. University of Saskatchewan; 2020. Available from: http://hdl.handle.net/10388/12821
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
4.
Mirabzadeh-Ardakani, Ali.
Characterization of DEFB103 gene structure, gene expression and its haplotype association with three common diseases in Beef and Holstein cattle.
Degree: 2016, University of Saskatchewan
URL: http://hdl.handle.net/10388/7277
► The DEFB103 gene is a member of the β-defensin gene family, and it has not been well studied in cattle. In this study DEFB103 gene…
(more)
▼ The DEFB103 gene is a member of the β-defensin gene family, and it has not been well studied in cattle. In this study DEFB103 gene expression, gene structure and its haplotypes association with three diseases were studied. DEFB103 gene expression was profiled for 27 tissues in nine two-week old calves and the transcript was most abundant in tissues with stratified squamous epithelium. An age-dependent decrease (P <0.05) in DEFB103 gene expression was observed in buccal epithelium when comparing healthy two-week old and 10- to 12-month-old calves. A bovine herpesvirus-1 respiratory infection did, however, significantly (P < 0.05) up-regulate DEFB103 gene expression in the buccal epithelium of 6- to 8-month-old calves. Immunohistochemistry was used to identify cells expressing DEFB103 protein within tissues with stratified squamous epitheliums to confirm the DEFB103 gene expression results. DEFB103 protein was most abundant in basal epithelial cells and was present in these cells before birth. A close association was observed between the dendritic cells and epithelial cells expressing DEFB103 in both the fetus and newborn calf, and this is consistent with the β-defensin regulatory effect on dendritic cell responses.
DEFB103 cDNA boundaries were determined using RT-PCR. A newly discovered non-coding exon 1a and a 261 bp intron 1a were identified in cattle. At least two complete copies of DEFB103 with an ATG start codon are present in cDNA in some cattle. DEFB103 sequence assemblies and partial cloning cloning sequences revealed two types of deletion (4-bp and 8-bp) in the 5′UTR.
Two novel SNPs were identified in the 5′UTR of the DEFB103 (
c.-383A>G,
c.-241G>A) in addition to five (
c.-319A>G,
c.-264C>T,
c.-69A>G,
c.-42A>G, and
c.-34G>A) previously reported SNPs in the 5′UTR of DEFB103 gene. The association among three diseases and four haploid haplotypes was studied. Diploid haplotype analysis shows a trend toward increased risk of mastitis (1/4; P= 0.053) in the
Saskatchewan population and a decreased risk of mastitis (4/4; P= 0.06) in the Pennsylvania population. An inconsistent trend toward decreased (2; P=0.09) or increased (4; P=0.09) risk of mastitis also were determined when the haploid haplotypes were compared to others in the
Saskatchewan and the Pennsylvania populations. There was no significant difference in the haplotype frequencies of the control group and cattle with Cancer-eye (P= 0.43). No significant difference was determined in the survival curve (P=0.50), the level of serum haptoglobin (P=0.65), or the level of interferon-gamma (P= 0.50) between cattle with diploid haplotypes 2/2 and 2/4 and shipping fever.
Advisors/Committee Members: Schmutz, Sheila M, Griebel, Philip, Laarveld, Bernard, Buchanan, Fiona C, Van Kessel, Andrew.
Subjects/Keywords: Copy number variation; Defensin; Haplotype; Splicing
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mirabzadeh-Ardakani, A. (2016). Characterization of DEFB103 gene structure, gene expression and its haplotype association with three common diseases in Beef and Holstein cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7277
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mirabzadeh-Ardakani, Ali. “Characterization of DEFB103 gene structure, gene expression and its haplotype association with three common diseases in Beef and Holstein cattle.” 2016. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/7277.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mirabzadeh-Ardakani, Ali. “Characterization of DEFB103 gene structure, gene expression and its haplotype association with three common diseases in Beef and Holstein cattle.” 2016. Web. 11 Apr 2021.
Vancouver:
Mirabzadeh-Ardakani A. Characterization of DEFB103 gene structure, gene expression and its haplotype association with three common diseases in Beef and Holstein cattle. [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/7277.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mirabzadeh-Ardakani A. Characterization of DEFB103 gene structure, gene expression and its haplotype association with three common diseases in Beef and Holstein cattle. [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/7277
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
5.
Ochoa Sanabria, Carlos Andres.
Feeding Fusarium-infected wheat to yellow mealworm larvae (Tenebrio Molitor) to produce a safe, replacement protein source for animal feed.
Degree: 2019, University of Saskatchewan
URL: http://hdl.handle.net/10388/11742
► Various insect species including the yellow mealworm (Tenebrio molitor) could be an alternative and sustainable source of protein for animal feed. There is evidence that…
(more)
▼ Various insect species including the yellow mealworm (Tenebrio molitor) could be an alternative and sustainable source of protein for animal feed. There is evidence that yellow mealworm larvae can utilize deoxynivalenol (DON)-contaminated wheat as a food source without sequestering it, producing a safe protein ingredient. This study aimed to determine the potential accumulation of DON in T. molitor larvae reared on Fusarium-infected wheat containing high levels of DON and investigate the effects of DON exposure on production, survival and nutritional traits of the larvae. Wheat containing 200 μg/kg DON was used as the control diet. A different source of wheat was sorted into six fractions and mixed to obtain three levels of DON for low (2,000 μg/kg), medium (10,000 μg/kg) and high (12,000 μg/kg) treatments. Each treatment was replicated five times with 300 or 200 mealworms per replicate for the feeding and breeding trials, respectively. Trial termination was determined when the first two pupae were observed (32-34 days). There was no difference in the levels of DON detected in the larvae between treatments and ranged from 121.8±19.3 to 136.4±40.5 μg/kg (P=0.883). Excretion of DON in pooled frass samples was 131.0, 324.0, 230.4 and 742.1 μg/kg for control, low, medium and high, respectively. The concentrations of 3-acetyldeoxynivalenol (3-ADON) detected in frass ranged from 279.5 to 326.4 μg/kg, whereas levels in larvae ranged from 65.3 to 66.2 μg/kg and were from undetectable to 204.9 μg/kg in wheat. Nutritional analysis on pooled samples from both trials showed maximum levels of crude protein (CP) of 52% and crude fat (CF) of 36%. Ash, fiber, chitin, fatty-acids and amino-acids content were consistent across treatments. Survival was greater than 96% for all life stages in both trials. In the feeding trial, average daily gain (ADG) ranged from 1.9±0.1 to 2.1±0.1 mg/day per mealworm. Less than 1.2% of the ingested DON was accumulated by larvae when they consumed Fusarium-infected wheat containing levels up to 12,000 μg/kg. These results along with the lack of effect on the nutritional profile, survival, or production traits, supports using DON-contaminated wheat in large-scale production of mealworms to produce a sustainable, safe protein source.
Advisors/Committee Members: Buchanan, Fiona C, Hogan, Natacha S, Brook, Ryan, Newkirk, Rex, Gillott, Cedric, Weber, Lynn.
Subjects/Keywords: Fusarium; deoxynivalenol; detoxification; survivability
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ochoa Sanabria, C. A. (2019). Feeding Fusarium-infected wheat to yellow mealworm larvae (Tenebrio Molitor) to produce a safe, replacement protein source for animal feed. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/11742
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ochoa Sanabria, Carlos Andres. “Feeding Fusarium-infected wheat to yellow mealworm larvae (Tenebrio Molitor) to produce a safe, replacement protein source for animal feed.” 2019. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/11742.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ochoa Sanabria, Carlos Andres. “Feeding Fusarium-infected wheat to yellow mealworm larvae (Tenebrio Molitor) to produce a safe, replacement protein source for animal feed.” 2019. Web. 11 Apr 2021.
Vancouver:
Ochoa Sanabria CA. Feeding Fusarium-infected wheat to yellow mealworm larvae (Tenebrio Molitor) to produce a safe, replacement protein source for animal feed. [Internet] [Thesis]. University of Saskatchewan; 2019. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/11742.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ochoa Sanabria CA. Feeding Fusarium-infected wheat to yellow mealworm larvae (Tenebrio Molitor) to produce a safe, replacement protein source for animal feed. [Thesis]. University of Saskatchewan; 2019. Available from: http://hdl.handle.net/10388/11742
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
6.
Hamilton, Kelsey Evelyn 1994-.
A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle.
Degree: 2019, University of Saskatchewan
URL: http://hdl.handle.net/10388/11739
► min A (VA) has a nutrigenomic effect on intramuscular fat. Discovering variants in genes involved in fat deposition that are also affected by vitamin A…
(more)
▼ min A (VA) has a nutrigenomic effect on intramuscular fat. Discovering variants in genes involved in fat deposition that are also affected by vitamin A could allow feedlots to precision feed to optimize carcass traits. A single nucleotide polymorphism (SNP) in alcohol dehydrogenase 1C (ADH1C) has previously shown promise for this approach but has yet to be effective at a commercial level; therefore we hypothesized a variant in another gene or its interaction with ADH1Cc.-64T>
C might be the solution. Genes previously shown to be affected by retinoic acid, a metabolite of vitamin A: aminopeptidase (ANPEP), clusterin (CLU), adipose differentiation-related protein (ADFP), glutathione peroxidase (GPX3), secreted protein, acidic, cysteine-rich (SPARC), and insulin growth factor binding protein 6 (IGFBP6) were sequenced and screened for variants. The ANPEPc.410G>A SNP was selected for genotyping in a population of mixed breed steers (n=988). This population was fed vitamin A at 100% (100VA) or 50% (50VA the NRC recommended level (2200 IU/kg dry matter). No interaction was found with ADH1Cc.-64T>
C however, ANPEPc.410G>A affected carcass yield (P<0.01; AA=2.47±0.03, GA=2.36±0.03, GG=2.14±0.08), marbling score (P<0.01; AA=397.2±2.7, GA=388.6±3.3, GG=370.4±7.2), and fat (P<0.01; AA=8.52±0.17 GA=7.58±0.21, GG=7.04±0.44; mm). Vitamin A also had an effect on backfat (P<0.05; 100VA= 8.13±0.24, 50VA = 7.35±0.25), and an interaction with ANPEPc.410c.G>A affected rib-eye area (P<0.05). The ANPEP SNP was genotyped in a second population of mixed breed steers (N=708) fed a standard feedlot ration with the NRC recommended level of vitamin A. There was an association with yield, marbling, fat, and rib-eye area (P<0.01). The AA genotype was more marbled, while GG animals were leaner with higher yields. Interestingly, ANPEPc.410G>A is the fourth variant in a haplotype containing twelve SNPs that are in linkage disequilibrium in exon 1 and intron 1. This was confirmed by sequencing cattle of various breeds from different populations. The three haplotypes could affect gene expression by altering transcription or translation efficiency. Investigation of the functional effects of these variants needs to be completed in order to understand how it alters traits related to feedlot cattle performance.
Advisors/Committee Members: Buchanan, Fiona C, Asai-Coakwell, Mika, McKinnon, John J, Bett, Kirstin E, Brook, Ryan K.
Subjects/Keywords: beef; cattle; genetics; variants; marbling; feedlot; carcass; nutrigenomics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hamilton, K. E. 1. (2019). A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/11739
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hamilton, Kelsey Evelyn 1994-. “A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle.” 2019. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/11739.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hamilton, Kelsey Evelyn 1994-. “A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle.” 2019. Web. 11 Apr 2021.
Vancouver:
Hamilton KE1. A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle. [Internet] [Thesis]. University of Saskatchewan; 2019. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/11739.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hamilton KE1. A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle. [Thesis]. University of Saskatchewan; 2019. Available from: http://hdl.handle.net/10388/11739
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
7.
Prystupa, Jaclyn Mercedes.
Genetic diversity in Canadian, mountain and moorland, and Nordic pony populations.
Degree: 2011, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-06202011-205904
► The legally binding international declaration of the Convention on Biological Diversity (signed by over 180 countries) recently acknowledged the importance of conserving genetic diversity within…
(more)
▼ The legally binding international declaration of the Convention on Biological Diversity (signed by over 180 countries) recently acknowledged the importance of conserving genetic diversity within livestock species. This study aimed to help Canada assess molecular diversity in its horse and pony (Equus ferus caballus) genetic resources. Here, 24 populations were examined, with special focus on the native Canadian, Mountain and Moorland, and Nordic pony populations, using two well accepted molecular tools. Additional horse breeds and feral populations were also included in this project as some may have influenced the development of the three equine groups of interest. Altogether, 821 individuals were genotyped at 38 microsatellite loci, and 280 individuals were sequenced using a 421 base pair portion of the mitochondrial displacement Hypervariable Region I.
Results from the microsatellite analyses indicated that 13.33% of genetic diversity arose from breed differences, whereas 84.60% and 2.07% of diversity arose from within and among individuals respectively. The New Forest and Welsh breeds were found to be the most diverse while having the highest average effective number of alleles and allelic richness (4.31 and 6.01; 4.33 and 5.87 respectively). The Eriskay and Lac La Croix breeds were found to have the lowest average effective number of alleles and allelic richness (2.51 and 3.98; 2.83 and 4.01 respectively). Expected heterozygosities were lowest in the Lac La Croix (0.61) and highest in the Welsh and New Forest (0.74) breeds, whereas observed heterozygosities were highest in the Kerry Bog (0.77) and lowest in the Exmoor (0.57) breeds. The genetic structure and admixture analyses suggested that the most probable number of unique genetic clusters was 21 as opposed to the 24 predefined populations.
Results from the mitochondrial sequence data revealed that there were 36 informative sites producing 62 haplotypes, 20 of which were previously unreported. The Connemara was found to have the highest haplotype diversity of the pony breeds (0.89); however, the Highland pony was found to have the highest nucleotide diversity and pairwise difference (0.16 and 6.73 respectively). In contrast, the Fell pony had the lowest haplotype diversity (0.22), and the feral Sable Island population had the lowest nucleotide diversity and pairwise difference (0.01 and 0.29 respectively). Multiple phylogenetic trees were reconstructed and produced similar topologies. In general, the Mountain and Moorland and Nordic breeds were spread among the clades, whereas native Canadian populations were most frequent in the D and E clades. Interestingly, a large portion of ponies were found within the rare E clade as opposed to horses.
Information gathered from this project can be incorporated with other available data into pre-existing conservation/breeding programs currently managed by the various breed societies to ensure that the most optimal and sustainable strategies are in place.
Advisors/Committee Members: Plante, Yves, Hill, Janet E., McLoughlin, Philip D., Laarveld, Bernard, Buchanan, Fiona C..
Subjects/Keywords: conservation; mtDNA; microsatellite; characterization; Equus ferus caballus
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Prystupa, J. M. (2011). Genetic diversity in Canadian, mountain and moorland, and Nordic pony populations. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06202011-205904
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Prystupa, Jaclyn Mercedes. “Genetic diversity in Canadian, mountain and moorland, and Nordic pony populations.” 2011. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/etd-06202011-205904.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Prystupa, Jaclyn Mercedes. “Genetic diversity in Canadian, mountain and moorland, and Nordic pony populations.” 2011. Web. 11 Apr 2021.
Vancouver:
Prystupa JM. Genetic diversity in Canadian, mountain and moorland, and Nordic pony populations. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/etd-06202011-205904.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Prystupa JM. Genetic diversity in Canadian, mountain and moorland, and Nordic pony populations. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/etd-06202011-205904
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
8.
Domolewski, Stacey Jo 1991-.
Sire verification in multi-sire breeding systems.
Degree: 2017, University of Saskatchewan
URL: http://hdl.handle.net/10388/7951
► An experiment was conducted to evaluate using DNA parentage testing on commercial cow-calf operations using multi-sire breeding groups to determine the associations between various traits…
(more)
▼ An experiment was conducted to evaluate using DNA parentage testing on commercial cow-calf operations using multi-sire breeding groups to determine the associations between various traits of bulls and number of calves sired. Four commercial
Saskatchewan ranches with 7 breeding groups collaborated in this study, where all potential sires and progeny were sampled to determine the sire of each calf. Expected vs observed calf data were analyzed using Chi-square analysis. In all but 2 of the 7 breeding groups, it was determined that each bull sired a different (P<0.01) number of calves. Age of sire was found to effect (P<0.01) bull prolificacy or number of calves sired. A bull prolificacy index (BPI) was developed to compare which bulls are siring more or less than expected number of progeny. All bulls were required to pass a breeding soundness exam (BSE) before breeding season, therefore weak correlations were found between scrotal circumference (R2=0.04) or percent normal sperm (R2=0.13) and BPI values. No sire match ranged from 2-7% of calves tested. Testing only calves born in the 3rd week of calving, indicated that bulls could be assigned accurately to high or low prolificacy categories, but bulls with the fewest number of calves sired could not be detected.
Economic models were developed to evaluate the value of adopting parentage testing on farm using a cost benefit analysis. The model showed bulls that sired more calves had a lower cost per calf sired. Based on the model, if bulls were siring < 25 calves it would be more economical to use AI in the breeding program. The model also determined that if one bull was causing increased dystocia rates in a herd, testing calves from difficult births to cull responsible bulls did provide an economic return on investment to the operation. There is potential to increase overall bull prolificacy in a herd and increase other economically important traits by using DNA parentage to aid in sire selection.
Advisors/Committee Members: Lardner , Bart A, Mutsvangwa, Tim, Buchanan, Fiona c, Campbell, John, Palmer, Colin.
Subjects/Keywords: Bull prolificacy; DNA parentage; sire verification
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Domolewski, S. J. 1. (2017). Sire verification in multi-sire breeding systems. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7951
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Domolewski, Stacey Jo 1991-. “Sire verification in multi-sire breeding systems.” 2017. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/7951.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Domolewski, Stacey Jo 1991-. “Sire verification in multi-sire breeding systems.” 2017. Web. 11 Apr 2021.
Vancouver:
Domolewski SJ1. Sire verification in multi-sire breeding systems. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/7951.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Domolewski SJ1. Sire verification in multi-sire breeding systems. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7951
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
9.
Wheeler, Amy Loy.
Habitat Selection by Parturient and Post-parturient Adult Female Moose (Alces alces) on the Canadian Prairies.
Degree: 2020, University of Saskatchewan
URL: http://hdl.handle.net/10388/12836
► The expansion of moose into the agricultural landscape of Saskatchewan (i.e., farmland moose) has increased human-wildlife conflicts, raising questions about how to best manage them.…
(more)
▼ The expansion of moose into the agricultural landscape of
Saskatchewan (i.e., farmland moose) has increased human-wildlife conflicts, raising questions about how to best manage them. To support decision making, I initiated a study on farmland moose reproductive success and habitat selection following parturition (i.e., birth of calves). In 2013 and 2014, adult female moose were captured between Saskatoon and Chamberlain, SK and fitted with Global Positioning System collars. Daily movement rates and clustering of locations were used to determine the date and location of parturition for 27 adult female moose from 2013 to 2015. The mean date of parturition was May 21. Moose were surveyed using Very High Frequency radio telemetry in June and September each year to visually determine the presence and number of calves. Of the pregnant females observed during calf surveys with 1 or 2 calves, twinning rates were 67% (n = 6/9) in June 2013 and 46% (n = 5/11) in June 2014. Habitat selection ratios indicated that wetland and riparian habitat, trees and shrubs, and cropland were selected the most strongly by female moose as parturition habitat, while pastures and forages, developed and native grassland habitat were avoided. Female moose selected parturition sites further away from roads. A resource selection function model was developed to quantify habitat selection by 15 female moose with young during the first 20 days post-parturition. During this period, adult female moose with young most strongly selected for wetland and riparian areas (β [95% CI] = 0.716 [0.485, 0.946]) and native grassland (β [95% CI] = 0.457 [0.329, 0.585]) and against oilseeds (β [95% CI] = –0.252 [–0.400, –0.103]). Predictive success of the top-ranked model, estimated from k-fold cross validation, was rs = 0.993 (SE = 0.001). The resource selection function indicates that only 10% of the area within the home ranges of parturient females is considered highly selected habitat with high probability of moose use, while 48% of the area has a low probability of use. These results demonstrate the importance of wetland habitat within cropland to female moose, during and shortly after giving birth.
Advisors/Committee Members: Brook, Ryan K, Buchanan, Fiona C, Hogan, Natacha S, Clark, Robert G, McCance, Erin C.
Subjects/Keywords: moose; parturition; habitat selection; resource selection
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wheeler, A. L. (2020). Habitat Selection by Parturient and Post-parturient Adult Female Moose (Alces alces) on the Canadian Prairies. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12836
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wheeler, Amy Loy. “Habitat Selection by Parturient and Post-parturient Adult Female Moose (Alces alces) on the Canadian Prairies.” 2020. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/12836.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wheeler, Amy Loy. “Habitat Selection by Parturient and Post-parturient Adult Female Moose (Alces alces) on the Canadian Prairies.” 2020. Web. 11 Apr 2021.
Vancouver:
Wheeler AL. Habitat Selection by Parturient and Post-parturient Adult Female Moose (Alces alces) on the Canadian Prairies. [Internet] [Thesis]. University of Saskatchewan; 2020. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/12836.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wheeler AL. Habitat Selection by Parturient and Post-parturient Adult Female Moose (Alces alces) on the Canadian Prairies. [Thesis]. University of Saskatchewan; 2020. Available from: http://hdl.handle.net/10388/12836
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
10.
Zalinko, Grant.
Effect of a low lignin hull, high oil groat oat on beef cattle growth, carcass quality and nutrient utilization.
Degree: 2014, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2014-08-1671
► A series of experiments were conducted to investigate the nutritional value of a new oat variety developed by the Crop Development Centre at the University…
(more)
▼ A series of experiments were conducted to investigate the nutritional value of a new oat variety developed by the Crop Development Centre at the
University of
Saskatchewan. Trials 1 and 2 evaluated performance of steers fed a low lignin hull, high oil groat (LLH-HOG) oat as a replacement for barley or corn. In trial 1, 400 steers were fed one of two diets with barley or the LLH-HOG oat at 37.8% of the diet DM. Dry matter intake was lower (P=0.02) and gain to feed improved (P0.01) for steers fed the oat-based diet. In trial 2, 240 steers were finished diets with barley, corn or the LLH-HOG oat at 88.2% of the finishing diet (DM). During finishing, steers on the oat diet had lower (P0.01) ADG, body and carcass (P<0.01) weights than barley or corn-fed cattle reflecting lower (P0.01) DMI.
In trial 3, 20 steers were fed one of seven diets consisting of barley silage and 0, 28, 56, or 84% LLH-HOG oat or barley grain (DM basis) to compare nutrient digestibility. Apparent DM, OM, ADF and NDF digestibility coefficients were lower (P<0.05) for LLH-HOG oat-based diets compared to barley-based diets. Apparent CP and EE digestibility coefficients were higher (P<0.05) for the LLH-HOG oat diets.
Trial 4 was conducted to assess ruminal fermentation differences between LLH-HOG oat- or barley-based finishing diets using four rumen cannulated steers. No diet effects (P>0.05) were observed for total ruminal VFA concentration or molar proportions of individual VFA however mean ruminal pH was lower (P=0.01) for steers fed the LLH-HOG oat-finishing diet. Further, the extent of pH decline in oat-fed cattle was greater (P<0.01) than for barley-fed cattle.
The results indicate that the energy value of the LLH-HOG oat is equivalent or superior to that of barley for growing cattle. However, further research is required to identify factors limiting feed intake of cattle fed this new oat type in finishing diets.
Advisors/Committee Members: McKinnon, John J., Christensen, David A., Penner, Greg B., Buchanan, Fiona C..
Subjects/Keywords: low lignin hull, high-oil groat oat; barley; corn; cattle performance; digestibility; carcass traits; fatty acids
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zalinko, G. (2014). Effect of a low lignin hull, high oil groat oat on beef cattle growth, carcass quality and nutrient utilization. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-08-1671
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zalinko, Grant. “Effect of a low lignin hull, high oil groat oat on beef cattle growth, carcass quality and nutrient utilization.” 2014. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2014-08-1671.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zalinko, Grant. “Effect of a low lignin hull, high oil groat oat on beef cattle growth, carcass quality and nutrient utilization.” 2014. Web. 11 Apr 2021.
Vancouver:
Zalinko G. Effect of a low lignin hull, high oil groat oat on beef cattle growth, carcass quality and nutrient utilization. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2014-08-1671.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zalinko G. Effect of a low lignin hull, high oil groat oat on beef cattle growth, carcass quality and nutrient utilization. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-08-1671
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
11.
Anez, Federico.
EFFECT OF ENERGY SUPPLEMENTATION FROM BY-PRODUCT FEED PELLETS ON PRODUCTIVITY AND NUTRIENT UTILIZATION OF CATTLE GRAZING STOCKPILED CRESTED WHEATGRASS (Agropyron cristatum L.).
Degree: 2013, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2013-09-1200
► Three experiments were conducted to determine the effects of source (experiment 1), frequency, and level (experiments 2 and 3) of energy supplementation on performance, forage…
(more)
▼ Three experiments were conducted to determine the effects of source (experiment 1), frequency, and level (experiments 2 and 3) of energy supplementation on performance, forage utilization and intake, productivity, rumen fermentation, and nutrient digestibility of growing beef cattle fed stockpiled forage. In experiment 1 (EXP1) and experiment 2 (EXP2), 45 cross bred yearling steers were managed on stockpiled crested wheatgrass pasture over 70 days during summer/fall of 2011 and 2012. Steers were stratified by IBW (EXP1 = 334±1.2 kg; EXP2 = 358±1.8 kg) and allocated randomly to 1 of 9 crested wheatgrass pastures (5 steers/pasture). Each pasture was randomly assigned to 1 of 3 replicated (n = 3) treatments. In EXP1, two isonitrogenous and isocaloric by-product feed pellets that differed in starch and degradable fiber content were used in one of three supplementation strategies: 1) no supplement (CON), or supplemented at 0.6 % of BW with 2) low starch/high fibre (LS/HF) pellet (40.3% starch; 29.5% NDF DM basis) pellet, or 3) high starch/low fibre (HS/LF; 48.6% starch; 22.8% NDF DM basis) pellet. In EXP2 a by-product feed pellet was formulated to provide ruminal and post-ruminal energy (30.3 % NDF; 32.0 % starch; 7.2 % fat) supplementation strategies included: 1) daily (DLY) supplementation at 0.6 % of BW, 2) low-alternate (LA) supplementation at 0.9 % of BW, and 3) high-alternate (HA) supplementation at 1.2 % of BW. There was no effect (P > 0.05) of treatment on forage utilization in either experiment. In EXP 1, final BW and ADG were not different (P > 0.05) between LS/HF (435 kg; 1.4 kg d-1) and HS/LF (439 kg; 1.5 kg d-1). However, supplemented cattle had higher (P < 0.05) final BW and ADG than CON cattle (402 kg; 1.0 kg d-1). Supplementation increased production costs by 450 %. In EXP 2, no difference (P > 0.05) was observed for final BW and ADG among DLY (435 kg; 1.1 kg d-1), LA (424 kg; 0.9 kg d-1), and HA (428 kg; 1.0 kg d-1). Production costs were reduced by 23 % with alternate supplementation and LA had 19 % less production costs than HA.
In experiment three (EXP 3), four ruminally cannulated beef heifers were individually fed a stockpiled grass hay and offered the same pelleted supplement as in EXP2. Treatments consisted of 4 supplementation strategies: 1) no supplement (CON), 2) daily (DLY) supplementation at 0.6% BW, 3) low-alternate (LA) supplementation at 0.9 % of BW, and 4) high-alternate (HA) supplementation at 1.2 % of BW. Forage intake, rumen fermentation parameters, and apparent total tract digestibility were measured. Three data sets were analyzed: 1) overall (average of all collection days), 2) day of supplementation (DS) and 3) non-supplementation day (NSD) for alternating treatments. Overall, hay DMI (kg d-1) was lower (P = 0.04) for DLY (7.1) vs. CON (8.1), but no different (P ≥ 0.11) for DLY vs. LA (6.9), or vs. HA (6.4). On DS, hay DMI (kg d-1) of DLY (7.3) differed (P < 0.05) vs. HA (6.0), but was not different (P = 0.16) vs. LA (6.4). On NSD, hay DMI (kg d-1) of DLY (7.0) was not different (P ≥…
Advisors/Committee Members: McKinnon, John J., Lardner, Herbert A., Penner, Greg B., Jefferson, Paul G., Buchanan, Fiona C..
Subjects/Keywords: Energy supplementation; beef cattle; stockpiled crested wheatgrass
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Anez, F. (2013). EFFECT OF ENERGY SUPPLEMENTATION FROM BY-PRODUCT FEED PELLETS ON PRODUCTIVITY AND NUTRIENT UTILIZATION OF CATTLE GRAZING STOCKPILED CRESTED WHEATGRASS (Agropyron cristatum L.). (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-09-1200
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Anez, Federico. “EFFECT OF ENERGY SUPPLEMENTATION FROM BY-PRODUCT FEED PELLETS ON PRODUCTIVITY AND NUTRIENT UTILIZATION OF CATTLE GRAZING STOCKPILED CRESTED WHEATGRASS (Agropyron cristatum L.).” 2013. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2013-09-1200.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Anez, Federico. “EFFECT OF ENERGY SUPPLEMENTATION FROM BY-PRODUCT FEED PELLETS ON PRODUCTIVITY AND NUTRIENT UTILIZATION OF CATTLE GRAZING STOCKPILED CRESTED WHEATGRASS (Agropyron cristatum L.).” 2013. Web. 11 Apr 2021.
Vancouver:
Anez F. EFFECT OF ENERGY SUPPLEMENTATION FROM BY-PRODUCT FEED PELLETS ON PRODUCTIVITY AND NUTRIENT UTILIZATION OF CATTLE GRAZING STOCKPILED CRESTED WHEATGRASS (Agropyron cristatum L.). [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2013-09-1200.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Anez F. EFFECT OF ENERGY SUPPLEMENTATION FROM BY-PRODUCT FEED PELLETS ON PRODUCTIVITY AND NUTRIENT UTILIZATION OF CATTLE GRAZING STOCKPILED CRESTED WHEATGRASS (Agropyron cristatum L.). [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-09-1200
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
12.
Anez Osuna, Federico 1976-.
LEVEL AND SOURCE OF FAT IN THE DIET OF BEEF COWS.
Degree: 2019, University of Saskatchewan
URL: http://hdl.handle.net/10388/12074
► Two studies were conducted to evaluate the effects of fat and the type of fatty acid (MUFA vs. PUFA) inclusion in the diet of beef…
(more)
▼ Two studies were conducted to evaluate the effects of fat and the type of fatty acid (MUFA vs. PUFA) inclusion in the diet of beef cows during the pre- and postpartum period on the performance of the dam and the progeny. In study 1, replicated over three years, 36 second- and third-calving lactating Angus cows were stratified by BW (554±15.5 kg) and days postpartum (38±1.5 d), and randomly assigned to 9 paddocks (4 cows/paddock) where cows grazed cool-season grass (CSG) pastures (12.5±2.5% CP and 56.5±2.9% TDN). Each paddock was randomly assigned to one of three replicated treatments: a non-supplemented control (CON), and two supplemented (SUP) treatments where cows were offered either a canola seed (CAN) or a flaxseed (FLX) based pellet targeting 300 g/cow/d of supplemental fat (EE) over 42 d. Data were analyzed as a RCBD with contrasts for the effect of fat supplementation (CON vs. SUP) and source (CAN vs. FLX). Results indicate that CON had greater (P=0.01) forage utilization and tended (P=0.08) to have greater estimated forage DMI compared to SUP, while no difference (P≥0.76) was observed between CAN and FLX. At the end of the trial, all treatments resulted in positive ADG, maintained or increased BCS and SCFT, and reduced serum NEFA concentration with no difference (P≥0.20) among treatments. No differences (P≥0.12) were observed for pregnancy rate, calving distribution and calving to calving interval. In study 2, replicated over 2 years, 75 multiparous (≥3 calving) pregnant Angus cows were stratified by BW (663±21.5 kg) and BCS (2.6±0.12), and randomly assigned to 15 outdoor pens. Subsequently, each pen was randomly assigned to one of three (n=5) treatments: a low-fat diet (LF; 1.4±0.12% EE) and two high-fat diets (HF; 3.3±0.20% EE) which included a CAN or a FLX pelleted feeds similar to those used in study 1. Diets were formulated to meet the requirements of pregnant beef cows during the last two trimesters of gestation (183±4.8 d), and offered such that each pen on average received similar amounts of DE (31.2±2.8 Mcal/cow/d), CP (1.36±0.13 kg/cow/d), and DM (12.9±1.0 kg/cow/d). Data were analyzed as RCBD with contrasts for the effects of level (LF vs. HF) and source (CAN vs. FLX) of fat. After 160 d on trial, conceptus corrected-BW (CC-BW) of LF cows (708 kg) and the proportion of over conditioned cows (13.2%) were greater (P≤0.04) than those of HF, with no difference (P≥0.84) between CAN and FLX. Feeding FLX diet over gestation resulted in subcutaneous adipose tissue (SCAT) with greater (P≤0.01) concentration of CLnA (0.12 vs. 0.05%) and n-3 (0.58 vs. 0.37%) fatty acids, and a tendency (P=0.09) for CLA concentration (1.05 vs. 0.88%) to be greater when compared to CAN diet. By the end of gestation, serum NEFA concentration of LF cows (592 μEq/L) was lower (P<0.01) than that of HF cows, and FLX cows had greater (P<0.01) serum NEFA concentration than CAN cows (636 vs. 961 μEq/L). Cows receiving the LF diet over gestation gave birth to lighter (P≤0.01) calves compared to those receiving the HF diets (40.2 vs.…
Advisors/Committee Members: McKinnon, John J, Lardner, Herbet A, Buchanan, Fiona C, Penner, Gregory B, Jefferson, Paul G, Campbell, John, Fitzsimmons, Carolyn J.
Subjects/Keywords: Beef cows; Gestation; Fat supplementation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Anez Osuna, F. 1. (2019). LEVEL AND SOURCE OF FAT IN THE DIET OF BEEF COWS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12074
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Anez Osuna, Federico 1976-. “LEVEL AND SOURCE OF FAT IN THE DIET OF BEEF COWS.” 2019. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/12074.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Anez Osuna, Federico 1976-. “LEVEL AND SOURCE OF FAT IN THE DIET OF BEEF COWS.” 2019. Web. 11 Apr 2021.
Vancouver:
Anez Osuna F1. LEVEL AND SOURCE OF FAT IN THE DIET OF BEEF COWS. [Internet] [Thesis]. University of Saskatchewan; 2019. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/12074.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Anez Osuna F1. LEVEL AND SOURCE OF FAT IN THE DIET OF BEEF COWS. [Thesis]. University of Saskatchewan; 2019. Available from: http://hdl.handle.net/10388/12074
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
13.
Krone, Kristin.
EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS.
Degree: 2014, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2014-06-1580
► Previously, ADH1Cc.-64T>C was shown to have an association with intramuscular fat (IMF) in the longissimus thoracis (LT) muscle when vitamin A was limited in finishing…
(more)
▼ Previously, ADH1Cc.-64T>
C was shown to have an association with intramuscular fat (IMF) in the longissimus thoracis (LT) muscle when vitamin A was limited in finishing rations of beef steers. The purpose of the current study was to determine the optimum vitamin A supplementation level, in combination with ADH1C genotype, to increase IMF of the LT muscle. Forty-five TT, 45 CT and 27 CC cross-bred steers, black in colour, were backgrounded on a commercial ration containing 3360 IU vitamin A/kg DM. During finishing the steers were randomly assigned to one of three vitamin A treatments at 25, 50 and 75% of the NRC recommendation of 2200 IU/kg DM. Treatments were administered via an oral bolus. Carcass quality was evaluated and a sample from the LT muscle was collected for analysis of IMF. A treatment x genotype interaction (P=0.04) was observed for IMF; TT steers on the 75% treatment had higher IMF relative to CT and CC steers on the same treatment. Intramuscular fat was also higher for TT steers on the 75% treatment in comparison to TT steers on the 25% treatment. Eighty-four percent of the steers graded Canada AAA. Western blot analysis showed that TT steers had higher (P=0.02) ADH1C levels in hepatic tissue. Previously, TT steers had increased IMF when fed limited vitamin A. In the current study the lack of variation between treatments and genotypes at the lower vitamin A treatment levels was likely due to the majority of the steers grading Canada AAA (USDA Choice). However, the western blot data supports that TT steers are expected to have higher IMF deposition, due to an increase production of ADH1C.
Advisors/Committee Members: Buchanan, Fiona C., McKinnon, John J., Hendrick, Steve, Hogan, Natacha.
Subjects/Keywords: Vitamin A; beef cattle; nutrigenetics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Krone, K. (2014). EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-06-1580
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Krone, Kristin. “EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS.” 2014. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2014-06-1580.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Krone, Kristin. “EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS.” 2014. Web. 11 Apr 2021.
Vancouver:
Krone K. EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2014-06-1580.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Krone K. EFFECT OF VARYING DIETARY VITAMIN A SUPPLEMENTATION LEVELS IN COMBINATION WITH ADH1C GENOTYPE ON INTRAMUSCULAR FAT DEPOSITION IN FINISHING BEEF STEERS. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-06-1580
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
14.
WIDYARATNE, GEMUNU.
Characterization and improvement of the nutritional value of ethanol by-products for swine.
Degree: 2005, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2005-11-418
► The nutritional value of distiller’s dried grains with solubles (DDGS) has not been assessed in swine. The nutritional value of corn and wheat DDGS, and…
(more)
▼ The nutritional value of distiller’s dried grains with solubles (DDGS) has not been assessed in swine. The nutritional value of corn and wheat DDGS, and possibilities to improve the nutritional value of wheat DDGS were for swine were investigated in two studies.
In study 1, two experiments were conducted to determine digestibility and digestible contents of energy, amino acids (AA) and P in corn and wheat DDGS and wheat grain, together with N and P excretion and growth performance in grower-finisher pigs. In experiment 1, 12 barrows (64.6 ± 6.4 kg) were fitted with ileal T-cannulae and had restricted access (2.6 x maintenance) to a wheat control diet or one of three diets with 40% corn, wheat+corn (4:1) or wheat DDGS. For energy, apparent total tract digestibility was highest for wheat (85%; P < 0.05) and did not differ among DDGS (77 to 79%; P > 0.10). Total tract digestible energy (DE) was highest for corn DDGS (4292 kcal kg-1 DM; P < 0.05) and tended to differ among wheat+corn and wheat DDGS and wheat (4038, 4019, and 3807, respectively; P = 0.06). For lysine, apparent ileal digestibility (AID) was highest for wheat (71%; P < 0.05) and did not differ among DDGS (59 to 63%; P > 0.10). The apparent ileal digestible lysine content was highest for corn DDGS (0.51% DM; P < 0.05), intermediate for wheat+corn and wheat DDGS (0.45 and 0.42), and lowest for wheat (0.37%). For P, total tract digestibility was lowest for wheat (15%; P < 0.05) and did not differ among DDGS samples (53 to 56%; P > 0.10). Total N excretion was highest for wheat+corn and wheat DDGS (55 and 58 g d-1; P < 0.05), intermediate for corn DDGS (44) and lowest for wheat (36). Total P excretion did not differ among DDGS (11 g d-1) and was lowest for wheat (8; P < 0.05). In experiment 2, 100 pigs (52.0 ± 3.3 kg) were fed a wheat-pea control diet or one of three 25%-DDGS (corn, wheat+corn or wheat) diets (3.375 Mcal DE kg-1; 2.50 g AID lysine Mcal-1 DE) for 5 wk. Overall, average daily feed intake (ADFI) and daily gain (ADG) were higher for wheat than DDGS (P < 0.05) but feed efficiency did not differ (P > 0.10). In summary, the nutritional value of wheat DDGS for swine is higher than wheat and lower than corn DDGS and feeding DDGS reduced growth performance, partly via a reduced ADFI, indicating that anti-nutritional factors in DDGS require further investigation.
In study 2, the effect of xylanase supplementation of wheat DDGS on nutrient digestibility and nutrient excretion was evaluated in grower-finisher pigs. Wheat-based diets with or without 40% wheat DDGS were tested with or without supplementary xylanase (4,000 U kg-1 feed) as a 2 x 2 factorial arrangement in a repeated Latin square design using eight barrows (29.4 ± 2.0 kg) fitted with ileal T-cannulae. Following a 6-day acclimation, faeces and urine were collected for 3 d, and ileal digesta for 2 d. The apparent ileal energy digestibility and DE content were not affected either by ingredient or xylanase (P > 0.05). The total tract energy digestibility and DE content were affected by ingredient…
Advisors/Committee Members: Zijlstra, Ruurd T., Christensen, David A., Drew, Murray D., Zello, Gordon, Buchanan, Fiona C..
Subjects/Keywords: DDGS; pig; digestibility; energy; amino acid; xylanase
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
WIDYARATNE, G. (2005). Characterization and improvement of the nutritional value of ethanol by-products for swine. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2005-11-418
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
WIDYARATNE, GEMUNU. “Characterization and improvement of the nutritional value of ethanol by-products for swine.” 2005. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2005-11-418.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
WIDYARATNE, GEMUNU. “Characterization and improvement of the nutritional value of ethanol by-products for swine.” 2005. Web. 11 Apr 2021.
Vancouver:
WIDYARATNE G. Characterization and improvement of the nutritional value of ethanol by-products for swine. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2005-11-418.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
WIDYARATNE G. Characterization and improvement of the nutritional value of ethanol by-products for swine. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/ETD-2005-11-418
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
15.
Huang, Xuewei.
Improvements in nutritive value of canola meal with pelleting.
Degree: 2015, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2015-02-1952
► Production of and demand for Canadian canola meal have been increased yearly. In order to improve the competitiveness of canola meal domestically and internationally, as…
(more)
▼ Production of and demand for Canadian canola meal have been increased yearly. In order to improve the competitiveness of canola meal domestically and internationally, as well as to develop potential markets for canola meal, it is necessary to develop canola meal-based products that have high feed values and can be easily transported. The objectives of this research were: 1) to investigate the effects of temperature and time of conditioning during pelleting process on the nutritive values of canola meal in terms of chemical profiles, protein and carbohydrate subfractions, and energy values, using the AOAC procedures, CNCPS v6.1 and NRC (2001), respectively; 2) to detect the effects of temperature and time of conditioning during the pelleting process on rumen degradation and intestinal digestion characteristics and predicted protein supply of canola meal, using the in situ procedure, the three-step in vitro procedure, and the NRC 2001 model; and 3) to determine pelleting-induced changes in spectral characteristics of molecular structures of canola meal using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) with univariate and multivariate analysis, and reveal the relationship between molecular structures of protein and carbohydrate and nutrient values, rumen degradation and intestinal digestion characteristics, and predicted protein supply of canola meal. Three different conditioning temperatures (70, 80 and 90ºC) and two different conditioning time (50 and 75 sec) were applied in this research. Two different batches of canola meal from a commercial feed company were selected. A randomized complete block design (RCBD) with 3 × 2 factorial arrangement was employed in this research. Molecular spectral functional groups related to protein, cellulosic compounds, and carbohydrates were used in the spectral study. This research indicated: 1) soluble crude protein (SCP) was decreased and neutral detergent insoluble CP (NDICP) was increased with increasing temperature; 2) the lowest protein rumen degradation of pellets was observed at conditioning temperature of 90 ºC and protein rumen degradation was increased by pelleting; 3) the amount of protein digested in the small intestine tended to increase with increasing conditioning temperature; 4) pelleting under different temperatures and time in the current study shifted the protein digestion site to the rumen, rather than to the small intestine; 5) with respect to predicted protein supply, based on the NRC 2001 model, increasing conditioning temperature tended to increase the metabolizable protein supply of canola meal pellets to dairy cattle; 6) changes in the molecular structure of canola meal induced by pelleting can be detected by ATR-FTIR; 7) not only protein molecular structure characteristics but also carbohydrate molecular structure characteristics play important roles in determining nutrient values, rumen degradation and intestinal digestion characteristics, and the predicted protein supply of canola meal.
Advisors/Committee Members: Yu, Peiqiang, Buchanan, Fiona C., Christensen, Colleen R., Scott, Tom A..
Subjects/Keywords: canola meal; pelleting; protein and carbohydrate; feed value; molecular structures; Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR); model prediction
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Huang, X. (2015). Improvements in nutritive value of canola meal with pelleting. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-02-1952
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Huang, Xuewei. “Improvements in nutritive value of canola meal with pelleting.” 2015. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2015-02-1952.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Huang, Xuewei. “Improvements in nutritive value of canola meal with pelleting.” 2015. Web. 11 Apr 2021.
Vancouver:
Huang X. Improvements in nutritive value of canola meal with pelleting. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2015-02-1952.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Huang X. Improvements in nutritive value of canola meal with pelleting. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-02-1952
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
16.
Ward, Alison.
Vitamin A and intramuscular fat deposition: a nutrigenetic investigation in beef cattle.
Degree: 2011, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2011-11-210
► min A restriction has been associated with increased marbling in beef cattle. The purpose of this study was to investigate a possible nutrigenetic mechanism leading…
(more)
▼ min A restriction has been associated with increased marbling in beef cattle. The purpose of this study was to investigate a possible nutrigenetic mechanism leading to this increase in intramuscular fat. Four genes involved in the vitamin A pathway were examined for genetic polymorphisms that could alter mRNA expression or protein structure. A total of fourteen SNPs were found in bovine alcohol dehydrogenase 1C (ADH1C), aldehyde dehydrogenase 1A1 (ALDH1A1), peroxisome proliferator-activated receptor (PPAR), and retinol binding protein 4 (RBP4). Five of these SNPs were examined for associations with production and carcass traits in a previously reported population of 400 crossbred steers. ALDH1A1
c.*27C>T was significantly associated with backgrounding average daily gain (P<0.05) however no associations were found between ADH1C
c.-64T>
C, ADH1C
c.967C>T, ALDH1A1
c.*109A>G, or PPAR
c.1344G>T with any of the parameters measured.
A nutritional study was performed to examine the interaction between ADH1C
c.-64T>
C and vitamin A restriction on production and carcass traits in cattle. It was hypothesized that a phenotypic effect would be observed only when vitamin A was restricted. An initial population of 450 black Angus cross steers were genotyped at ADH1C
c.-64T>
C and from that population 130 steers (50 TT, 50 CT, and 30 CC) were randomly selected for use in the vitamin A restriction study. They were backgrounded for three months on a low -carotene diet and finished for five months on a diet that did not contain supplemental vitamin A. During the finishing period the steers were treated with monthly boluses of either 0 IU (unsupplemented) or 750,000 IU (supplemented) supplemental vitamin A. Liver and fat biopsies were obtained at the start and end of finishing from a subgroup of five steers per genotype per treatment to assess vitamin A status and measure gene expression. Marbling score was significantly greater (P<0.05) in unsupplemented steers. A significant interaction was found between genotype and vitamin A supplementation on intramuscular fat content. Within the unsupplemented treatment, TT steers had significantly greater intramuscular fat than CC steers. Within TT steers, unsupplemented steers had significantly greater intramuscular fat than supplemented animals. Expression of ADH1C in the liver was significantly higher in TT steers than CC steers. ADH1C
c.-64T>
C in combination with reduced vitamin A supplementation could potentially be implemented in marker-assisted management to maximize intramuscular fat deposition in finishing cattle.
Advisors/Committee Members: Buchanan, Fiona C., Schmutz, Sheila S., McKinnon, John J., Laarveld, Bernard, Palmer, Colin.
Subjects/Keywords: Genetics; Cattle nutrition; Vitamin A
…was approved by the University of Saskatchewan Animal Care Committee
(protocol number…
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ward, A. (2011). Vitamin A and intramuscular fat deposition: a nutrigenetic investigation in beef cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2011-11-210
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ward, Alison. “Vitamin A and intramuscular fat deposition: a nutrigenetic investigation in beef cattle.” 2011. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2011-11-210.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ward, Alison. “Vitamin A and intramuscular fat deposition: a nutrigenetic investigation in beef cattle.” 2011. Web. 11 Apr 2021.
Vancouver:
Ward A. Vitamin A and intramuscular fat deposition: a nutrigenetic investigation in beef cattle. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2011-11-210.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ward A. Vitamin A and intramuscular fat deposition: a nutrigenetic investigation in beef cattle. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/ETD-2011-11-210
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
17.
Mansfield, Graeme.
Evaluation of Intestinal Responses to Alternative Protein Sources for Rainbow Trout (Oncorhynchus mykiss).
Degree: 2015, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2015-12-2357
► Replacement of fish meal as the primary protein source in diets for farmed carnivorous fish is a major priority for sustainability of the aquaculture industry.…
(more)
▼ Replacement of fish meal as the primary protein source in diets for farmed carnivorous fish is a major priority for sustainability of the aquaculture industry. Three plant-based protein sources (soybeans, field peas, and canola) were investigated to compare their effects on the health and performance of rainbow trout (Oncorhynchus mykiss) and to identify significant anti-nutritional factors (ANFs). Six separate 8-week studies were conducted, over a period of one year, to assess the effects of protein source and processing level (meal versus protein concentrate) at dietary inclusion rates of 0 to 300 g kg-1.
Abundance of inflammatory and immune marker transcripts including proliferating cell nuclear antigen (PCNA), immunoglobulin M (IgM), interleukin-1 beta (IL-1β), interleukin-8 (IL-8), and interleukin-10 (IL-10) was evaluated in distal intestinal tissue by quantitative PCR (qPCR) analysis. Activity of the pro-apoptotic enzyme caspase-3 was also assayed in distal intestinal tissue. Transcript abundance was highly variable and no suitable genes for the internal normalization of qPCR data could be identified. As a result, transcript copy numbers were reported per 50 ng of total RNA. At 300 g kg-1 inclusion, soybean meal (SBM) increased abundance of IL-8 and IgM, pea meal (PM) increased abundance of IL-10, and canola protein concentrate (CPC) increased abundance of IL-8. Pea protein concentrate (PPC) reduced IL-8 abundance and caspase-3 activity, while increasing abundance of IL-10. Canola meal (CM) and soy protein concentrate (SPC) did not significantly affect the transcript abundance of any assayed gene. Pearson correlation coefficients were determined between gene transcript abundance, performance parameters, protein source, inclusion level, and ANF content. Specific growth rate (SGR) was negatively correlated with the abundance of IL-1β and IgM. Dietary inclusion of SBM was positively correlated with all assayed proinflammatory markers and negatively correlated with SGR. Inclusion of PM was positively correlated with both SGR and the abundance of IL-10. The inclusion of CM was negatively correlated with average daily feed intake (ADFI) and with the abundance of both IL-8 and PCNA. Inclusion of PPC correlated positively with SGR and negatively with the activity of caspase-3. Correlation between transcript abundance and dietary content of putative ANFs suggested negative correlations between glucosinolate content, proinflammatory cytokine expression, SGR, and ADFI; whereas, isoflavone content was positively correlated with proinflammatory markers and negatively correlated with SGR.
In conclusion, although high SBM and CM inclusion levels have been associated with reduced growth performance in trout, only SBM was associated with increased abundance of inflammatory marker transcripts. These contrasting responses may be mediated by CM glucosinolates, which could negatively affect palatability without inducing a pro-inflammatory response. Dietary PM was very well tolerated and may have promoted anti-inflammatory…
Advisors/Committee Members: Van Kessel, Andrew G., Drew, Murray D., Hill, Janet E., Buchanan, Fiona C., Marchant, Tracy A..
Subjects/Keywords: Rainbow trout; Plant-based protein; Inflammation; Quantitative PCR; Antinutritional factor; Protein concentrate
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mansfield, G. (2015). Evaluation of Intestinal Responses to Alternative Protein Sources for Rainbow Trout (Oncorhynchus mykiss). (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-12-2357
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mansfield, Graeme. “Evaluation of Intestinal Responses to Alternative Protein Sources for Rainbow Trout (Oncorhynchus mykiss).” 2015. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2015-12-2357.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mansfield, Graeme. “Evaluation of Intestinal Responses to Alternative Protein Sources for Rainbow Trout (Oncorhynchus mykiss).” 2015. Web. 11 Apr 2021.
Vancouver:
Mansfield G. Evaluation of Intestinal Responses to Alternative Protein Sources for Rainbow Trout (Oncorhynchus mykiss). [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2015-12-2357.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mansfield G. Evaluation of Intestinal Responses to Alternative Protein Sources for Rainbow Trout (Oncorhynchus mykiss). [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-12-2357
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
18.
Duncombe, Jillian L. 1990-.
AN EVALUATION OF GENE INTERACTIONS AFFECTING CARCASS YIELD AND MARBLING IN BEEF CATTLE.
Degree: 2016, University of Saskatchewan
URL: http://hdl.handle.net/10388/7369
► Genotype-specific management of beef cattle in feedlots has the potential to improve carcass uniformity. Gene variants affecting marbling include LEPc.73C>T, ADH1Cc.-64T>C, TG5, and GALR2c.-199T>G while…
(more)
▼ Genotype-specific management of beef cattle in feedlots has the potential to improve carcass uniformity. Gene variants affecting marbling include LEPc.73C>T, ADH1Cc.-64T>
C, TG5, and GALR2c.-199T>G while those in CRHc.22C>G, POMCc.288C>T, MC4Rc.856C>G and IGF2c.-292C>T influence lean yield. The purpose of the current study was to assess combinations of marbling gene variants with those associated with lean yield and to investigate the effects of a gene variant in serotonin receptor 1B (HTR1B) on beef carcass traits. Gene variants were initially genotyped in 386 crossbred steers and evaluated for associations with carcass traits (hot carcass weight, average fat, grade fat and rib-eye area). The goal was to select a subset of variants to genotype in 2000 steers (1000 with hormone implants and 1000 without implants) with camera graded carcass data (Vision USDA yield grade, Vision grade marbling, rib-eye area and fat thickness). Seven gene variants were selected to proceed with (TG was discontinued) as they either had an association or were involved in gene interactions affecting a trait. In the implanted steers GALR2 affected rib-eye area (P=0.002) where it exhibited an additive effect (TT=83.74 cm2, TG= 84.32 cm2 and GG=86.90 cm2) however there was a dominant effect of the T allele for marbling (P=0.0001; TT/TG = 397.83 and GG=378.27) and fat (P=0.001; TT/TG=8.38 mm and GG=7.31). This same association with marbling (P<0.0001; TG/TT 463.52 mm and GG=430.90) and fat (P=0.006; TT/TG = 10.23 mm and GG=9.14 mm) was also observed in the non-implanted steers where again the T allele showed dominance. Gene-gene interactions affecting a trait were only observed in the non-implanted steers with the multivariate analysis: LEPc.73C>T and IGF2c.-292C>T with fat (P=0.05) and a trend with marbling (P=0.07); MC4Rc.856C>G and POMCc.288C>T with marbling (P=0.05); and GALR2c.-199T>G and POMCc.288C>T with rib-eye area (P=0.03). Associations between gene variants with traits were made simpler due to the fact that some genotypes could be collapsed, as least square means (LSM) were not significantly different, indicating a dominant effect of one allele. The ability to pool genotypes not only simplified the interactions, it resulted in a larger number of animals with combined genotypes. The gene SNP networks generated using EPISNP support the mode of action between gene variants. For example, the gene interaction that was a 3 by 2 was also determined to be Additive-Dominance.
Significant associations were also identified between HTRIB
c.205G>T SNP with carcass average fat (P=0.001), grade fat (P=0.007) and cutability (P=0.001) and a trend was observed with carcass REA (P=0.061). Although finding significance with several economically important carcass traits in crossbred beef breeds is novel, validating the effects of the HTRIB
c.205G>T SNP in a larger cattle population would be beneficial.
Advisors/Committee Members: Buchanan, Fiona C, Schmutz, Sheila M, McKinnon, John, Mutsvangwa, Timothy, Shand, Phyllis.
Subjects/Keywords: beef cattle; gene interactions
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APA ·
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MLA ·
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CSE |
Export
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APA (6th Edition):
Duncombe, J. L. 1. (2016). AN EVALUATION OF GENE INTERACTIONS AFFECTING CARCASS YIELD AND MARBLING IN BEEF CATTLE. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7369
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Duncombe, Jillian L 1990-. “AN EVALUATION OF GENE INTERACTIONS AFFECTING CARCASS YIELD AND MARBLING IN BEEF CATTLE.” 2016. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/7369.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Duncombe, Jillian L 1990-. “AN EVALUATION OF GENE INTERACTIONS AFFECTING CARCASS YIELD AND MARBLING IN BEEF CATTLE.” 2016. Web. 11 Apr 2021.
Vancouver:
Duncombe JL1. AN EVALUATION OF GENE INTERACTIONS AFFECTING CARCASS YIELD AND MARBLING IN BEEF CATTLE. [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/7369.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Duncombe JL1. AN EVALUATION OF GENE INTERACTIONS AFFECTING CARCASS YIELD AND MARBLING IN BEEF CATTLE. [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/7369
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
19.
lamichhane, santosh.
EVALUATION OF VACUUM POST-PELLET APPLICATIONS OF BIOACTIVES TO BROILER FEED ON EFFICACY AND PROTECTED DELIVERY.
Degree: 2015, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2015-07-2099
► The use of vacuum coating is mostly limited to production of high fat containing extruded aqua and pet diets. The physical characteristics of extrudates are…
(more)
▼ The use of vacuum coating is mostly limited to production of high fat containing extruded aqua and pet diets. The physical characteristics of extrudates are favourable for vacuum coating due to their high porosity and durability. However, with pelleted feed for broilers, there are potentially several opportunities, but there are also challenges; these are explored here. The opportunities identified were inclusion of high level of oils, protected delivery of feed additives (e.g., enzymes, probiotics, vaccines, etc.), improved and safe use of offensive feed additives and improvement of shelf-life of feed and additives. Challenges include the relatively high density of pellets (low porosity) which limits liquid infusion, increased processing cost and decreased feed throughput. However, feed ingredients selection and alternating processing variables (temperature, moisture, die specifications etc.) were deemed to overcome the challenges of low porosity. Three experiments were conducted to evaluate the use of vacuum coating in pelleted feed. In the first experiment, the effect of particle size on post-pellet oil absorption (OA), porosity, pellet durability index (PDI) and bulk density were investigated. The three particle sizes for three grains (wheat, barley and corn) were pelleted using a 4.7 mm die to get whole grain (WP), coarse (CP), and fine (FP) grind pellets. The pellets were coated with 15% canola oil without (VC-) and with (VC+; 0.3 bar) vacuum coating. The grain type was found to have a significant effect on the particle size when ground through either fine (3.2 mm) or coarse (6.4 mm) screen. With coarse grinding, the mean particle size was 1896, 1290 and 1057 µm, respectively for barley, wheat and corn; with fine grinding, the mean particle size was 1153, 767 and 732 µm, respectively. Porosity of CP from wheat and corn was significantly (P<0.01) higher than WP and FP. For barley, there was no difference in porosity of CP and FP but both were significantly higher than WP. For wheat, OA of CP was highest (P<0.01), but no significant difference was found between FP and WP. However, for barley, higher OA was found in FP followed by CP and WP. In corn, OA of CP was higher than for FP or WP. Vacuum coating (VC+) improved (P<0.01) OA of all pellets compared to VC-. Porosity was positively correlated with OA and negatively correlated to PDI and bulk density. Overall, the first experiment suggested that alteration of particle size and grain type could be the options for improving the oil absorption by vacuum coating. A second experiment was conducted to observe the effect of enzyme addition method (EAM; E-, without enzyme; PreE+, Pre-pellet addition of enzyme; PosE+, post-pellet addition of enzyme), conditioning temperature (CT; 65 or 95°
C) and coating method (CM; VC- or VC+) on broiler performance when fed wheat-rye-based diets. Enzyme addition (pre or post-pellet addition in comparison to without enzyme) significantly improved (P<0.01) the body weight at 21 and 35d. Higher CT (95°
C) improved feed…
Advisors/Committee Members: Scott, Thomas A., Classen, Henry L., Buchanan, Fiona C., Van Kessel, Andrew, Tyler, Robert T..
Subjects/Keywords: Bioactives; Pellet durability; Pellet porosity; Vacuum coating
…Christopher University of Saskatchewan,
Saskatoon, SK, personal communication, 2013).
In the…
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
lamichhane, s. (2015). EVALUATION OF VACUUM POST-PELLET APPLICATIONS OF BIOACTIVES TO BROILER FEED ON EFFICACY AND PROTECTED DELIVERY. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-07-2099
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
lamichhane, santosh. “EVALUATION OF VACUUM POST-PELLET APPLICATIONS OF BIOACTIVES TO BROILER FEED ON EFFICACY AND PROTECTED DELIVERY.” 2015. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2015-07-2099.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
lamichhane, santosh. “EVALUATION OF VACUUM POST-PELLET APPLICATIONS OF BIOACTIVES TO BROILER FEED ON EFFICACY AND PROTECTED DELIVERY.” 2015. Web. 11 Apr 2021.
Vancouver:
lamichhane s. EVALUATION OF VACUUM POST-PELLET APPLICATIONS OF BIOACTIVES TO BROILER FEED ON EFFICACY AND PROTECTED DELIVERY. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2015-07-2099.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
lamichhane s. EVALUATION OF VACUUM POST-PELLET APPLICATIONS OF BIOACTIVES TO BROILER FEED ON EFFICACY AND PROTECTED DELIVERY. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-07-2099
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
Sorensen, Anja.
Habitat selection by sympatric ungulates in an agricultural landscape : implications for disease transmission and human-wildlife conflict.
Degree: 2013, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2013-01-1390
► As areas of agricultural production expand worldwide, complex zones of wildlife-agriculture interface present numerous benefits and challenges to farmers and wildlife managers. In western Canada,…
(more)
▼ As areas of agricultural production expand worldwide, complex zones of wildlife-agriculture interface present numerous benefits and challenges to farmers and wildlife managers. In western Canada, free-ranging elk (Cervus canadensis), mule deer (Odocoileus hemionus), and white-tailed deer (Odocoileus virginianus) make frequent use of cereal, oilseed, and pulse crops. However, cervid use of annual crops presents substantial socio-economic concerns for producers. Additionally, use of crops may facilitate cervids co-mingling and increase the risk of intra- and inter-specific transmission of chronic wasting disease (CWD).
The purpose of my thesis research was to determine the key environmental factors influencing the selection of agricultural crops by elk, white-tailed deer, and mule deer, analyze overlap in species’ selection, and develop predictive models to identify the spatial distribution of crop damage risk. In this study, I analyzed 19,069 damage claims paid by
Saskatchewan Crop Insurance Corporation to
Saskatchewan farmers for confirmed losses to annual crops (cereals, oilseeds, pulses) from 2000-2012 by elk, mule deer, and white-tailed deer. These data were used to conduct species-specific ecological niche factor analyses (ENFAs), which relate habitat variables within damaged sites to that of the surrounding landscape. The key habitat variables influencing selection of annual crops were then incorporated into resource selection probability function (RSPF) models. These models characterize and predict the probability of crop damage by elk, mule deer, and white-tailed deer, and each possible dual species combination. By integrating damage probability values and historical monetary values of regional crop production, I evaluated the risk of annual crop damage by each of the three species, and dual species combinations, across all sections of agricultural land in
Saskatchewan.
The ENFAs revealed that elk and white-tailed deer selected for areas where a high proportion of farmland is seeded to oats, barley, canola, and alfalfa, while avoiding areas farther from protected areas, with a high density of paved or unpaved roads and a high proportion of open grassland. Alternately, mule deer favoured open grasslands, shrublands, and areas with a greater density of streams or water bodies, while avoiding areas where a high proportion of farmland is seeded to oats, canola, flaxseed, wheat, and barley. Areas at highest risk for annual crop damage by elk bordered the northern edge of the study area; mule deer damage risk was highest in south-western and central
Saskatchewan; while white-tailed deer damage risk was highest in north-eastern and north-central areas of the province.
Identifying these specific associations between landscape variables, rates of crop damage, and associated species overlap may provide an important opportunity for agencies to develop cooperative management strategies to efficiently allocate mitigation resources. Efforts to prevent the selection of cereal, oilseed, and pulse crops by free ranging elk,…
Advisors/Committee Members: Brook, Ryan K., Penner, Gregory, Lardner, Herbert, Buchanan, Fiona C., Schmutz, Josef K..
Subjects/Keywords: Habitat selection; elk; mule deer; white-tailed deer; Saskatchewan; crop damage
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sorensen, A. (2013). Habitat selection by sympatric ungulates in an agricultural landscape : implications for disease transmission and human-wildlife conflict. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-01-1390
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sorensen, Anja. “Habitat selection by sympatric ungulates in an agricultural landscape : implications for disease transmission and human-wildlife conflict.” 2013. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2013-01-1390.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sorensen, Anja. “Habitat selection by sympatric ungulates in an agricultural landscape : implications for disease transmission and human-wildlife conflict.” 2013. Web. 11 Apr 2021.
Vancouver:
Sorensen A. Habitat selection by sympatric ungulates in an agricultural landscape : implications for disease transmission and human-wildlife conflict. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2013-01-1390.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sorensen A. Habitat selection by sympatric ungulates in an agricultural landscape : implications for disease transmission and human-wildlife conflict. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-01-1390
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
21.
Yang, Ling.
EFFECT OF CARBOHYDRATE TRAITS ON NUTRITIONAL CHARACTERISTICS AND.
Degree: 2012, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2012-12-849
► Four hulless barley varieties (zero-amylose waxy, CDC Fibar; 5%-amylose waxy, CDC Rattan; normal-amylose, CDC McGwire and high-amylose, HB08302) were developed at the Crop Development Centre,…
(more)
▼ Four hulless barley varieties (zero-amylose waxy, CDC Fibar; 5%-amylose waxy, CDC Rattan; normal-amylose, CDC McGwire and high-amylose, HB08302) were developed at the Crop Development Centre,
University of
Saskatchewan with differences in carbohydrates traits on the basis of amylose (1 to 20% DM), amylopectin (34 to 51% DM), amylose to amylopectin ratio (0.02 to 0.59) and β-glucan (5 to 10% DM) content. The objectives of this research were to determine: 1) the effect of the alteration of these carbohydrate traits in hulless barley on nutrient availability in ruminants, and 2) spectral characteristics of molecular structures in comparison with hulled barley-CDC Copeland. Studies on chemical and nutrient profiles, rumen degradation kinetics, in vitro intestinal nutrient digestion and potential protein supply estimated by the Dutch model and the NRC Dairy 2001 model were carried out. Fourier Transform Infrared Spectroscopy (FTIR) and advanced synchrotron-based FTIR Microspectroscopy (SR-FTIRM) with univariate and multivariate analysis were applied to investigate the influence of genetic modification of barley cultivars on the molecular structure features at the regions of protein amide I and II, β-glucan, cellulosic compounds and carbohydrates. By quantifying the relationship between the measured parameters and the alteration of carbohydrate traits, the results of studies revealed: 1) the hulless barley lines with altered carbohydrate traits have the potential to increase rumen and intestinal nutrient availability, thus improving the truly absorbed protein supply to ruminants compared to hulled barley; 2) lower amylose and higher β-glucan level in the hulless barley varieties increased estimated energy and metabolizable protein supply to ruminants; 3) molecular structure differences of the hulless barley varieties can be detected by both conventional FTIR spectroscopy and SR-FTIRM; 4) metabolizable protein (MP) was affected significantly by protein molecular structure characteristics in hulless barley.
Advisors/Committee Members: Yu, Peiqiang, Christensen, David A., McKinnon, John J., Buchanan, Fiona C..
Subjects/Keywords: Hulled and hulless barley; Amylose; β-Glucan; DVE/OEB; Synchrotron-based Fourier Transform Infrared Spectroscopy (SR-FTIRM)
…were developed at the
Crop Development Centre, University of Saskatchewan, based on amylose…
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yang, L. (2012). EFFECT OF CARBOHYDRATE TRAITS ON NUTRITIONAL CHARACTERISTICS AND. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-12-849
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yang, Ling. “EFFECT OF CARBOHYDRATE TRAITS ON NUTRITIONAL CHARACTERISTICS AND.” 2012. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2012-12-849.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yang, Ling. “EFFECT OF CARBOHYDRATE TRAITS ON NUTRITIONAL CHARACTERISTICS AND.” 2012. Web. 11 Apr 2021.
Vancouver:
Yang L. EFFECT OF CARBOHYDRATE TRAITS ON NUTRITIONAL CHARACTERISTICS AND. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2012-12-849.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yang L. EFFECT OF CARBOHYDRATE TRAITS ON NUTRITIONAL CHARACTERISTICS AND. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-12-849
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
22.
Wiebe, Krystalee.
Molecular characterization of Cdu-B1, a major locus controlling cadmium accumulation in durum wheat (Triticum turgidum L. var durum) grain.
Degree: 2012, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2012-09-621
► A major gene controlling grain cadmium (Cd) concentration, designated as Cdu-B1, has been mapped to the long arm of chromosome 5B, but the genetic factor(s)…
(more)
▼ A major gene controlling grain cadmium (Cd) concentration, designated as Cdu-B1, has been mapped to the long arm of chromosome 5B, but the genetic factor(s) conferring the low Cd phenotype are currently unknown. Genetic mapping of markers linked to Cdu-B1 in a population of recombinant inbred substitution lines (RSLs) revealed that the gene(s) associated with variation in Cd concentration reside(s) in wheat deletion bin 5BL9 between fraction breakpoints 0.76 and 0.79, and linked to two candidate genes; PCS2 (phytochelatin synthetase) and Xwg644, which codes for a known ABC (ATP-binding cassette) protein. Genetic mapping and quantitative trait locus (QTL) analysis of grain Cd concentration was performed in a doubled haploid (DH) population and revealed that these genes were not associated with Cdu-B1. Two expressed sequence markers (ESMs), and five sequence tagged site (STS) markers were identified that co-segregated with Cdu-B1, and explained >80% of the phenotypic variation in grain Cd concentration. A gene coding for a P1B-ATPase, designated as OsHMA3 (heavy metal associated), has recently been associated with phenotypic variation in grain Cd concentration in rice. Mapping of the orthologous gene to OsHMA3 in the DH population revealed complete linkage with Cdu-B1 and was designated as HMA3-B1. Fine mapping of Cdu-B1 in >4000 F2 plants localized Cdu-B1 to a 0.14 cM interval containing HMA3-B1. Two bacterial artificial chromosomes (BACs) containing full-length coding sequence for HMA3-B1 and HMA3-A1 (homoeologous copy from the A genome) were identified and sequenced. Sequencing of HMA3-B1 from high and low Cd accumulators of durum wheat revealed a 17 bp duplication in high accumulators that results in predicted pre-mature stop codon and thus, a severely truncated protein. Several DNA markers linked to Cdu-B1, including HMA3-B1, were successfully converted to high throughput markers and were evaluated for practical use in breeding programs. These markers were successful at classifying a collection of 96 genetically diverse cultivars and breeding lines into high and low Cd accumulators and will have broad application in breeding programs targeting selection for low grain Cd concentrations. Current results support HMA3-B1 as a candidate gene responsible for phenotypic differences in grain Cd concentrations in durum wheat.
Advisors/Committee Members: Pozniak, Curtis J., Bett, Kirstin E., Scoles, Graham J., Coulman, Bruce E., Buchanan, Fiona C..
Subjects/Keywords: cadmium; fine mapping; HMA
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wiebe, K. (2012). Molecular characterization of Cdu-B1, a major locus controlling cadmium accumulation in durum wheat (Triticum turgidum L. var durum) grain. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-09-621
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wiebe, Krystalee. “Molecular characterization of Cdu-B1, a major locus controlling cadmium accumulation in durum wheat (Triticum turgidum L. var durum) grain.” 2012. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/ETD-2012-09-621.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wiebe, Krystalee. “Molecular characterization of Cdu-B1, a major locus controlling cadmium accumulation in durum wheat (Triticum turgidum L. var durum) grain.” 2012. Web. 11 Apr 2021.
Vancouver:
Wiebe K. Molecular characterization of Cdu-B1, a major locus controlling cadmium accumulation in durum wheat (Triticum turgidum L. var durum) grain. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/ETD-2012-09-621.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wiebe K. Molecular characterization of Cdu-B1, a major locus controlling cadmium accumulation in durum wheat (Triticum turgidum L. var durum) grain. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-09-621
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Bryan, Dervan DSL 1986-.
CHARACTERIZATION OF PROTEIN SOURCES AND THEIR EFFECTS ON BROILER PERFORMANCE, DIGESTIVE TRACT MORPHOLOGY AND CAECAL FERMENTATION METABOLITES.
Degree: 2018, University of Saskatchewan
URL: http://hdl.handle.net/10388/8635
► The goal of this thesis was to characterize protein sources and evaluate their effects on broiler performance, digestive tract morphology and caecal fermentation. Understanding the…
(more)
▼ The goal of this thesis was to characterize protein sources and evaluate their effects on broiler performance, digestive tract morphology and caecal fermentation. Understanding the digestion kinetics of high protein ingredients is an important step in elucidating the impact protein sources have on poultry performance and health. An in vitro assay was used to characterize the digestion kinetics of feed ingredients fed to broilers. The assay predicted the rapidly, slowly and undigested protein fraction of a variety of ingredients, and their digestion rate and extent. Compared to corn distiller dried grain with solubles, soybean meal and fish meal crude protein (CP) was digested more rapidly and to a larger extent. The rate and extent of protein digestion in feed ingredients were also evaluated using broiler chickens, and further the ileal digesta CP was characterized. Protein sources varied in rate and extent of CP and amino acid (AA) digestibility, distal ileum digesta residual AA, and total and soluble CP content. Fish meal, corn gluten meal and soybean meal had similar CP and AA extent of digestion in vivo. Among the protein sources, fish meal had the highest digestion rate for most AA. The total and soluble CP in the distal ileal content of birds fed protein sources ranged from 54 to 1466 and 6 to 347 mg, respectively.
When broilers were vaccinated for coccidiosis and fed antibiotic free diets, dietary protein level (PL) and the ratio of indigestible protein (IDP) fraction affected broiler performance and meat yield. The disease status of the birds and the level of digestible AA in the diets influenced the bird’s response to dietary IDP. The level of caecal metabolites depended on dietary protein source, disease status of the birds and PL. In conclusion, data generated on in vitro and in vivo protein digestion kinetics provide an opportunity to categorize feed ingredients and use this information to establish the impact of these characteristics on broiler nutrition. Dietary PL and IDP affected broiler performance and meat yield when birds were vaccinated for coccidiosis and this was related to the characteristics of the protein sources present in the diets.
Advisors/Committee Members: Classen, Henry L, Nickerson, Michael T, Mutsvangwa, Timothy, Van Kessel, Andrew G, Newkirk, Rex w, Buchanan, Fiona C.
Subjects/Keywords: Indigestible protein; Protein digestion kinetics; In vitro and In vivo digestion; Protein digestion rate; Broiler chickens; Protein caecal fermentation; Growth performance; Coccidiosis vaccination; Antibiotic-free diet; Meat yield; Ross 308; Polyamine; Short chain fatty acids; Digestible amino acids
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bryan, D. D. 1. (2018). CHARACTERIZATION OF PROTEIN SOURCES AND THEIR EFFECTS ON BROILER PERFORMANCE, DIGESTIVE TRACT MORPHOLOGY AND CAECAL FERMENTATION METABOLITES. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/8635
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bryan, Dervan DSL 1986-. “CHARACTERIZATION OF PROTEIN SOURCES AND THEIR EFFECTS ON BROILER PERFORMANCE, DIGESTIVE TRACT MORPHOLOGY AND CAECAL FERMENTATION METABOLITES.” 2018. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/8635.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bryan, Dervan DSL 1986-. “CHARACTERIZATION OF PROTEIN SOURCES AND THEIR EFFECTS ON BROILER PERFORMANCE, DIGESTIVE TRACT MORPHOLOGY AND CAECAL FERMENTATION METABOLITES.” 2018. Web. 11 Apr 2021.
Vancouver:
Bryan DD1. CHARACTERIZATION OF PROTEIN SOURCES AND THEIR EFFECTS ON BROILER PERFORMANCE, DIGESTIVE TRACT MORPHOLOGY AND CAECAL FERMENTATION METABOLITES. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/8635.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bryan DD1. CHARACTERIZATION OF PROTEIN SOURCES AND THEIR EFFECTS ON BROILER PERFORMANCE, DIGESTIVE TRACT MORPHOLOGY AND CAECAL FERMENTATION METABOLITES. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/8635
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
24.
Good, Adriane Catherine 1993-.
EVALUATION OF CANOLA MEAL VERSUS SOYBEAN MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE: EFFECTS ON GROWTH PERFORMANCE, CARCASS CHARACTERISTICS, RUMEN FERMENTATION, AND NUTRIENT DIGESTION.
Degree: 2018, University of Saskatchewan
URL: http://hdl.handle.net/10388/8529
► The objective of this research was to determine the effect of canola meal (CM) as a protein supplement for beef cattle on growth performance, rumen…
(more)
▼ The objective of this research was to determine the effect of canola meal (CM) as a protein supplement for beef cattle on growth performance, rumen fermentation, and nutrient digestion compared to soybean meal (SBM) and wheat dried distillers’ grains with solubles (WDDGS). A 95-d backgrounding trial (Trial 1) and a 61-d backgrounding, 147-d finishing trial (Trial 2) were used to evaluate performance and carcass characteristics of feedlot cattle fed CM vs. SBM with or without WDDGS. In Trial 1, cattle fed SBM had greater ADG (P < 0.05) relative to cattle fed SBM+WDDGS but also numerically the highest feed cost of gain compared to the other three treatments. No other treatment differences were noted in Trial 1. In Trial 2, no treatment differences (P > 0.05) were detected for overall ADG, DMI, or gain : feed. Numerically, cattle fed SBM had the highest feed cost of gain with cattle fed WDDGS the lowest. Cattle fed SBM+WDDGS had the poorest fat deposition (P < 0.05) compared to cattle fed CM+WDDGS and WDDGS. However, no treatment differences were noted in final carcass value. A third trial using omasal, rumen, and fecal collections in heifers fed CM or SBM with or without WDDGS in a 4 x 4 Latin square was carried out to determine the effect of protein supplement on rumen fermentation, apparent and true ruminal nutrient digestibility, and total tract nutrient digestibility. Heifers fed WDDGS had lower (P < 0.05) DM, OM, and N intake than those not fed WDDGS. Heifers fed CM had the highest (P < 0.05) DM, OM, and N apparently and truly digested in the rumen compared to heifers fed SBM, and inclusion of WDDGS tended (P < 0.10) to decrease N truly digested in the rumen. There were no treatment differences (P > 0.05) noted in DM, OM, CP, ADF, or NDF digestibility. The results of all three trials indicate that CM is not different than SBM as protein supplement for feedlot cattle and that the inclusion of WDDGS did not improve feedlot performance, rumen fermentation, or nutrient digestibility.
Advisors/Committee Members: McKinnon, John J, Campbell, John R, Penner, Greg B, McAllister, Tim A, Mutsvangwa, Tim, Buchanan, Fiona C.
Subjects/Keywords: canola meal; wheat dried distillers' grains with solubles; soybean meal; beef cattle
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APA ·
Chicago ·
MLA ·
Vancouver ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Good, A. C. 1. (2018). EVALUATION OF CANOLA MEAL VERSUS SOYBEAN MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE: EFFECTS ON GROWTH PERFORMANCE, CARCASS CHARACTERISTICS, RUMEN FERMENTATION, AND NUTRIENT DIGESTION. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/8529
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Good, Adriane Catherine 1993-. “EVALUATION OF CANOLA MEAL VERSUS SOYBEAN MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE: EFFECTS ON GROWTH PERFORMANCE, CARCASS CHARACTERISTICS, RUMEN FERMENTATION, AND NUTRIENT DIGESTION.” 2018. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/8529.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Good, Adriane Catherine 1993-. “EVALUATION OF CANOLA MEAL VERSUS SOYBEAN MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE: EFFECTS ON GROWTH PERFORMANCE, CARCASS CHARACTERISTICS, RUMEN FERMENTATION, AND NUTRIENT DIGESTION.” 2018. Web. 11 Apr 2021.
Vancouver:
Good AC1. EVALUATION OF CANOLA MEAL VERSUS SOYBEAN MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE: EFFECTS ON GROWTH PERFORMANCE, CARCASS CHARACTERISTICS, RUMEN FERMENTATION, AND NUTRIENT DIGESTION. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/8529.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Good AC1. EVALUATION OF CANOLA MEAL VERSUS SOYBEAN MEAL AS A PROTEIN SUPPLEMENT FOR BEEF CATTLE: EFFECTS ON GROWTH PERFORMANCE, CARCASS CHARACTERISTICS, RUMEN FERMENTATION, AND NUTRIENT DIGESTION. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/8529
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
25.
Ma, Wai Kwong.
A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil.
Degree: 2004, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-01292004-111928
► Arbuscular mycorrhizal fungi (AMF) are important components of agro-ecosystems and are especially significant for productive low-input agriculture. Traditional spore morphology-based identification of AMF in biodiversity…
(more)
▼ Arbuscular mycorrhizal fungi (AMF) are important components of agro-ecosystems and are especially significant for productive low-input agriculture. Traditional spore morphology-based identification of AMF in biodiversity studies is subjective and requires expertise and time. Researchers have used molecular techniques to investigate community composition of AMF in uncultivated, disturbed, or contaminated soils, but this approach to community analysis of AMF in agricultural soils has not been reported. In this study, a polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) procedure for the detection of fungal 18S ribosomal RNA gene was developed with reference cultures. Five AMF species were procured from the International Culture Collection of Arbuscular and Vesicular-Arbuscular Mycorrhizal Fungi (INVAM). These reference cultures were chosen because isolates of their species were putatively identified in a previous survey of farm field soils in
Saskatchewan, Canada. A reference PCR-DGGE profile was generated using DNA extracted and amplified from the spores of these INVAM cultures. The method’s technical limitations were investigated. The optimized procedure’s effectiveness was tested by its application to soil samples from 38 farms. Bands from the PCR-DGGE profiles of these samples were excised for sequence analysis. The total number of species recovered was low in comparison to other AMF community surveys of temperate climate locations. The majority of the sequences recovered were Glomus species. Scutellospora calospora, a previously undetected AM fungus in
Saskatchewan was found. A trend in AMF distribution in
Saskatchewan was observed and it was relatable to their phylogenetic taxonomy. Though not without its drawbacks, this approach to community composition analysis of AMF was faster than conventional trap cultivation methods.
Advisors/Committee Members: Germida, James J., Walley, Frances L., Buchanan, Fiona C..
Subjects/Keywords: PCR; AMF; DGGE
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ma, W. K. (2004). A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-01292004-111928
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ma, Wai Kwong. “A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil.” 2004. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/etd-01292004-111928.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ma, Wai Kwong. “A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil.” 2004. Web. 11 Apr 2021.
Vancouver:
Ma WK. A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil. [Internet] [Thesis]. University of Saskatchewan; 2004. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/etd-01292004-111928.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ma WK. A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil. [Thesis]. University of Saskatchewan; 2004. Available from: http://hdl.handle.net/10388/etd-01292004-111928
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
26.
Sebastian, Thomas.
Temperament in beef cattle : methods of measurement, consistency and relationship to production.
Degree: 2007, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-12112007-213618
► Two behavioural studies were conducted at the University of Saskatchewan beef feedlot. In the first study, the temperament of 400 steers was determined using both…
(more)
▼ Two behavioural studies were conducted at the
University of
Saskatchewan beef feedlot. In the first study, the temperament of 400 steers was determined using both objective and subjective measures. The consistency of temperament, over repeated tests and between different measures, was also tested. The objective behavioural tests were conducted during the individual restraint of the steers using strain gauges and an MMD (movement-measuring-device). The time required for the steers to exit the area was also recorded. Subjective assessment of animals’ responsiveness during restraint was recorded on a scale of 1-5 (calm to wild). The consistency of individual differences in a steer’s response within the evaluation series and across repetitions, shows that this trait may represent a stable ‘personality’ of the animal. The significant relationship between objective and subjective measures demonstrates that objective measures of temperament can be used to replace the traditional subjective scale as it has the added advantage of reducing inter- and intra-observer variability. The positive relationship of subjective scores and MMD values with the steers performance (average daily weight gain) shows not only that a calm temperament is conducive to productivity, but also that objective measures can replace subjective techniques for assessing temperament for performance evaluation. In the second study the reactivity of a subset of the original 400 steers (262 animals from 8 pens) to a novel stimulus was assessed. The purpose of this experiment was to determine if a steers’ behavioural response in the novel test was correlated to its’ temperament assessment determined in the first study. A remote controlled ball was dropped from the ceiling of a salt feeder while a steer licked the salt. Two overhead cameras connected to a monitor through a VCR and time lapse recorder permitted us to observe and document the response. A lack of correlation between measures of handling and novelty measures show that reactivity of animals in the handling chute and their responsiveness to the novel stimulus do not represent one and the same trait.
Advisors/Committee Members: Stookey, Joseph M., McKinnon, John J., Gonyou, Harold W., Carruthers, Terry D., Buchanan, Fiona C..
Subjects/Keywords: movement measuring device; strain gauge; exit time; temperament; beef cattle
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sebastian, T. (2007). Temperament in beef cattle : methods of measurement, consistency and relationship to production. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12112007-213618
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sebastian, Thomas. “Temperament in beef cattle : methods of measurement, consistency and relationship to production.” 2007. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/etd-12112007-213618.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sebastian, Thomas. “Temperament in beef cattle : methods of measurement, consistency and relationship to production.” 2007. Web. 11 Apr 2021.
Vancouver:
Sebastian T. Temperament in beef cattle : methods of measurement, consistency and relationship to production. [Internet] [Thesis]. University of Saskatchewan; 2007. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/etd-12112007-213618.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sebastian T. Temperament in beef cattle : methods of measurement, consistency and relationship to production. [Thesis]. University of Saskatchewan; 2007. Available from: http://hdl.handle.net/10388/etd-12112007-213618
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
27.
Asiamah, Patience Agyarko.
Effects of single nucleotide polymorphisms in leptin and pro-opiomelanocortin on peripheral eucocyte counts in beef cattle.
Degree: 2005, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-09142005-202307
► Single nucleotide polymorphisms (SNP) in leptin (LEP) and pro-opiomelanocortin (POMC) have been associated with beef carcass quality and yield respectively. Both hormones also play a…
(more)
▼ Single nucleotide polymorphisms (SNP) in leptin (LEP) and pro-opiomelanocortin (POMC) have been associated with beef carcass quality and yield respectively. Both hormones also play a role in immune performance. Since both of these genes are pleiotrophic, it was important to determine whether selection based on these SNPs would negatively affect immune cell numbers. A SNP in each of these hormones was assessed for effects on immune cell counts and antibody titres in twenty-seven beef cattle herds (n = 556). A commercial rabies vaccine was administered to these animals. Prior to being vaccinated, the types of lymphocytes evaluated included B cells, gamma delta cells, regular and activated CD4 and CD8 cells and numbers of lymphocytes as well as baseline serum antibody titres. On day 21, antibody titres were measured and a booster vaccine was administered. Finally on day 42, antibody titres and lymphocyte types were again counted. Several cell types were significantly associated with the LEP genotype however, no consistent pattern of correlation was observed between LEP genotype (TT, CT or CC) and peripheral blood lymphocyte populations. The number of different lymphocytes significantly associated with LEP genotype increased from two on day 0 to four on day 42. Animals with CT and CC genotypes had significantly higher increased rabies antibody titres in the first 21 days after vaccination than those with TT genotypes. The POMC SNP also did not show a clear pattern of association between lymphocyte subtypes and genotype. There was no difference in response to the rabies vaccination associated with the POMC genotype. Our results suggested that selection at either of the SNPs examined in this research would not detrimentally impact immune function in beef cattle.
Advisors/Committee Members: Buchanan, Fiona C., Waldner, Cheryl, Van Kessel, Andrew G., Laarveld, Bernard, Bett, Kirstin E..
Subjects/Keywords: Leptin; Pro-opiomelanocortin; Single nucleotide polymorphism; Lymphocytes
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Asiamah, P. A. (2005). Effects of single nucleotide polymorphisms in leptin and pro-opiomelanocortin on peripheral eucocyte counts in beef cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-09142005-202307
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Asiamah, Patience Agyarko. “Effects of single nucleotide polymorphisms in leptin and pro-opiomelanocortin on peripheral eucocyte counts in beef cattle.” 2005. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/etd-09142005-202307.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Asiamah, Patience Agyarko. “Effects of single nucleotide polymorphisms in leptin and pro-opiomelanocortin on peripheral eucocyte counts in beef cattle.” 2005. Web. 11 Apr 2021.
Vancouver:
Asiamah PA. Effects of single nucleotide polymorphisms in leptin and pro-opiomelanocortin on peripheral eucocyte counts in beef cattle. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/etd-09142005-202307.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Asiamah PA. Effects of single nucleotide polymorphisms in leptin and pro-opiomelanocortin on peripheral eucocyte counts in beef cattle. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-09142005-202307
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
28.
Nilson, Stephanie Anne.
Stabilization of linseed oil for use in aquaculture feeds.
Degree: 2008, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-12012008-143546
► An experiment was conducted to determine the effect of addition of antioxidants or encapsulation of linseed oil on the oxidative stability of linseed oil and…
(more)
▼ An experiment was conducted to determine the effect of addition of antioxidants or encapsulation of linseed oil on the oxidative stability of linseed oil and the effect on growth and fatty acid composition of rainbow trout fed these products. Four diets differing only in their lipid sources were prepared by cold extrusion: 1) fish oil (FO), 2) linseed oil (LO), 3) linseed oil (980 g/kg) stabilized with vitamin E (7.5 g/kg) and butylated hydroxytoluene (BHT) (12.5 g/kg) (stabilized linseed oil; SLO) and 4) linseed oil (350 g/kg) containing vitamin E (7.5 g/kg), BHT (12.5 g/kg) and encapsulated in a coating material primarily consisting of hydrogenated palm oil (630 g/kg) (encapsulated linseed oil; ELO). Diets were fed twice daily to rainbow trout to apparent satiation (n=22 / replicate; 7 replicates per treatment) during a 168 day growth trial. Following the growth trial, the fish were humanely euthanized by a sharp blow to the cranium and analyzed for fatty acid composition, thiobarbituraric reactive substances (TBARS), fillet colour and sensory attributes (trained and consumer panels). There were no significant differences between treatments on any of the growth parameters investigated or TBARS levels of fish fillets. Omega-3 polyunsaturated fatty acids of trout fed LO were significantly higher than those fed FO (35.5% of total fatty acids vs. 27.6%) and ELO (28.9%) (P < 0.05). EPA and DHA levels were not significantly different between treatments. Diet samples were stored for 168 days at room temperature in sealed plastic containers. Following storage, the oxidative stability index (OSI) of the FO and LO diets were reduced to 0.00 hours while that of the SLO diet 9.20 hours and the ELO diet was 11.40 hours. Trained panelists determined fish fed FO had a significantly higher aroma intensity and significantly lower aroma desirability and overall acceptability than those fed SLO. The rancid aroma and flavour of the FO-fed fish was significantly higher than fish fed the other treatments (P < 0.05). Consumer panelists found no significant differences between the sensory attributes of fish fed the four experimental diets and exhibited no preference between treatments (P > 0.05). Fillets from fish fed FO had significantly higher values than the other three treatments for redness (3.59 vs values between 1.86 and 2.07) and yellowness (25.35 vs values between 20.51 and 21.22) (P < 0.05). Addition of antioxidants to linseed oil improves its oxidative stability during storage and processing and results in fish fillets with fatty acid composition and consumer acceptance equal or superior to fish fed fish oil.
Advisors/Committee Members: Drew, Murray D., Shand, Phyllis J., McKinnon, John J., Laarveld, Bernard, Buchanan, Fiona C..
Subjects/Keywords: Rainbow trout; Aquaculture feed; Linseed oil; Butylated hydroxytoluene; Encapsulation; Vitamin E
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Nilson, S. A. (2008). Stabilization of linseed oil for use in aquaculture feeds. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12012008-143546
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nilson, Stephanie Anne. “Stabilization of linseed oil for use in aquaculture feeds.” 2008. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/etd-12012008-143546.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nilson, Stephanie Anne. “Stabilization of linseed oil for use in aquaculture feeds.” 2008. Web. 11 Apr 2021.
Vancouver:
Nilson SA. Stabilization of linseed oil for use in aquaculture feeds. [Internet] [Thesis]. University of Saskatchewan; 2008. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/etd-12012008-143546.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nilson SA. Stabilization of linseed oil for use in aquaculture feeds. [Thesis]. University of Saskatchewan; 2008. Available from: http://hdl.handle.net/10388/etd-12012008-143546
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
29.
Penner, Gregory Brent.
The effect of pre-partum diet on the severity of post-partum ruminal acidosis in primiparous dairy cows.
Degree: 2006, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-08312006-062719
► Two experiments were conducted. In experiment 1, the objectives were: 1) to develop and evaluate the accuracy and precision of a new continuous ruminal pH…
(more)
▼ Two experiments were conducted. In experiment 1, the objectives were: 1) to develop and evaluate the accuracy and precision of a new continuous ruminal pH measurement system 2) to determine the required frequency for pH electrode standardization and 3) to determine the effect of additional pre-partum concentrate when compared to NRC (2001) recommendations on post-partum ruminal acidosis in primiparous cows. Accuracy and precision of the Lethbridge Research Centre Ruminal pH measurement system (LRCpH) was determined by comparing LRCpH derived values against manual measurement. To determine the required frequency of electrode standardization, three treatments were imposed (24, 48, or 72 h of continuous measurement) and arranged in a repeated 3×3 Latin square design. The LRCpH accurately and precisely measured ruminal pH (repeated measures correlation coefficient = 0.97 and concordance correlation coefficient = 0.97 for 5-min averages). Changes in baseline mV readings for pH readings after 24, 48 or 72 h of ruminal incubation were not significantly different than zero, indicating that daily standardization of new electrodes was not essential. Using the LRCpH to measure ruminal pH overcomes animal mobility restrictions of previous systems. In experiment 2, the effect of additional concentrate allocation during the pre-partum period was evaluated using 14 ruminally cannulated Holstein heifers. The heifers were assigned to one of two feeding regimes pre-calving: 1) control treatment or 2) an intensive high concentrate feeding treatment (HC). All cows received the same lactation diet post-partum. Ruminal pH was measured continuously from d -5 to d +5, and for 3-consecutive days starting on d +17 ± 1.2, d +37 ± 1.4, and d +58 ± 1.5 relative to parturition. Feeding additional concentrate pre-partum did not reduce post-partum ruminal acidosis. In fact, animals fed the HC treatment had more daily episodes of acute acidosis and lower dry matter intake and body condition score than animals fed the control treatment. Day relative to parturition affected the occurrence and severity of ruminal acidosis with a dramatic increase in ruminal acidosis after parturition. This study demonstrates that feeding addition concentrate pre-partum did not reduce post-partum acidosis which emphasized the need to develop and implement feeding strategies that reduce this risk.
Advisors/Committee Members: Mutsvangwa, Tim, Beauchemin, Karen A., Van Kessel, Andrew G., Christensen, David A., Buchanan, Fiona C..
Subjects/Keywords: Acidosis; Dairy cow; Rumen pH
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Penner, G. B. (2006). The effect of pre-partum diet on the severity of post-partum ruminal acidosis in primiparous dairy cows. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08312006-062719
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Penner, Gregory Brent. “The effect of pre-partum diet on the severity of post-partum ruminal acidosis in primiparous dairy cows.” 2006. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/etd-08312006-062719.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Penner, Gregory Brent. “The effect of pre-partum diet on the severity of post-partum ruminal acidosis in primiparous dairy cows.” 2006. Web. 11 Apr 2021.
Vancouver:
Penner GB. The effect of pre-partum diet on the severity of post-partum ruminal acidosis in primiparous dairy cows. [Internet] [Thesis]. University of Saskatchewan; 2006. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/etd-08312006-062719.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Penner GB. The effect of pre-partum diet on the severity of post-partum ruminal acidosis in primiparous dairy cows. [Thesis]. University of Saskatchewan; 2006. Available from: http://hdl.handle.net/10388/etd-08312006-062719
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan
30.
Ling, Binbing.
Study of hepatic cytochrome P450 system in Richardson ground squirrels.
Degree: 2005, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-12132005-121057
► Richardson ground squirrels (gophers) are pests on the prairies that cause considerable agricultural and ecological damage. Traditional control methods such as the rodenticides strychnine, zinc…
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▼ Richardson ground squirrels (gophers) are pests on the prairies that cause considerable agricultural and ecological damage. Traditional control methods such as the rodenticides strychnine, zinc phosphide, and anticoagulants, have proven ineffective in reducing gopher densities. In additional, current gopher control methods have the significant potential to cause primary and secondary toxicity to non-target animals. Thus, alternative methods for toxicological control of gophers are needed to mitigate these concerns. Present studies focused on the cytochrome P450 (CYP450) enzyme system responsible for xenobiotic detoxification in gophers. In vitro hepatic microsomal systems and HPLC analysis were used to elucidate general metabolic characteristics of major gopher xenobiotic metabolizing pathways. We found that the content and activity of individual components of the CYP450 system including CYP450, cytochrome b5, and NADPH-cytochrome P450 reductase in liver microsomal preparations were higher in gophers exposed to toxins used to control their population than in naïve (unexposed) gophers. When in vitro CYP450 mediated activities for five substrates [coumarin and aniline aromatic hydroxylation, 7-methoxycouamrin O-demethylation, and N-methylaniline, and N,N-dimethylaniline N-demethylation] were measured, naïve gophers were identified to have higher specific activity but similar whole body activity compared to the exposed gophers. Furthermore, there was a clearly identifiable sub-population of “poor metabolizers” showing considerably lower CYP450 activity within the gopher samples studied. Clotrimazole was found to be a potent inhibitor of several substrates of CYP450 enzyme-mediated reactions, which included aniline aromatic hydroxylation, N-methylaniline and N,N-dimethylaniline N-demethylation, and 7-methoxycoumarin O-demethylation. The cytotoxicity of above compounds was tested using freshly isolated gopher hepatocytes. The results showed that each compound caused considerable cytotoxicity to gopher hepatocytes. Addition of clotrimazole to the freshly isolated hepatocyte suspension increased the cytotoxicity of all tested compounds. In conclusion, gophers may develop resistance to current chemical control methods through the enhancement of CYP450 system content, which can compensate the loss of enzyme activity. Furthermore, clotrimazole is a potent cytochrome P450 inhibitor, which increases the cytotoxicity caused by given compounds in gopher livers. The concept of using CYP450 enzyme inhibitor in combination with another chemical whose elimination depends on CYP450 metabolism to improve current gopher control method has practical importance.
Advisors/Committee Members: Olkowski, Andrew A., Messier, François, Laarveld, Bernard, Janz, David M., Buchanan, Fiona C., Alcorn, Jane.
Subjects/Keywords: Cytochrome P450; Richardson ground squirrels
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APA (6th Edition):
Ling, B. (2005). Study of hepatic cytochrome P450 system in Richardson ground squirrels. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12132005-121057
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ling, Binbing. “Study of hepatic cytochrome P450 system in Richardson ground squirrels.” 2005. Thesis, University of Saskatchewan. Accessed April 11, 2021.
http://hdl.handle.net/10388/etd-12132005-121057.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ling, Binbing. “Study of hepatic cytochrome P450 system in Richardson ground squirrels.” 2005. Web. 11 Apr 2021.
Vancouver:
Ling B. Study of hepatic cytochrome P450 system in Richardson ground squirrels. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10388/etd-12132005-121057.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ling B. Study of hepatic cytochrome P450 system in Richardson ground squirrels. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-12132005-121057
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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