+publisher:"University of Saskatchewan" +contributor:("Bandy, Brian")

University of Saskatchewan

1. Peng, Fei. Mitochondrial uptake of anthocyanidins and protection from oxidative stress.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-08-626

► The anthocyanins show efficient antioxidant properties and free radical scavenging properties which result in various health-promoting benefits. This research investigated the ability of anthocyanidins to… (more)

▼ The anthocyanins show efficient antioxidant properties and free radical scavenging properties which result in various health-promoting benefits. This research investigated the ability of anthocyanidins to distribute into mitochondria and protect mitochondria from oxidative stress. In an in vitro study, the uptake of pure cyanidin and quercetin, and their 3-glucosylated forms into isolated rat liver mitochondria was tested, along with their effects on mitochondrial oxidative stress parameters. The absorption of cyanidin was significantly higher (67% uptake of 125 µM) than the other three flavonoids. Measurements indicated that the cyanidin was taken up into or tightly bound by mitochondria. Also, results suggested that cyanidin uptake was partially dependent on membrane potential. When incubated together (internally and externally) with mitochondria all tested flavonoids decreased reactive oxygen species (ROS) generation during mitochondrial respiration, and inhibited lipid peroxidation to different extents. Importantly, pre-loaded CY showed much stronger effects against oxidative stress in two analyses than other flavonoids. Due to its greater uptake by mitochondria, cyanidin may provide greater protection in vivo. In an in vivo study, cyanidin, quercetin and their 3-glucosides were administered into rat tail vein to give a dose of 7.6 µmol/Kg body weight. Cyanidin and its glucoside had greater affinity to liver and kidney than did quercetin and its glucoside; particularly, all test tissues contained a significantly higher amount of cyanidin than other test flavonoids. Also, cyanidin accumulated more in liver mitochondria than other flavonoids, and consistent with in vitro results was present in mitochondria to a much greater extent than cyanidin glucoside. However, delivery of the flavonoids at this dose did not significantly affect the liver mitochondria susceptibility to lipid peroxidation or the level of endogenous tissue oxidative damage. Altogether the results show that cyanidin can rapidly and efficiently accumulate in mitochondria, wherein it exhibits strong bio-antioxidant activity against oxidative stress and may help protect mitochondrial function and integrity. Also, the anthocyanidin and its 3-glucoside have greater ability than flavonols to accumulate in organs; especially cyanidin presented in liver mitochondria to a much greater extent. Cyanidin could be a potent natural antioxidant compound that is effective in mitochondria-protective therapies. Advisors/Committee Members: Bandy, Brian, Alcorn, Jane, Low, Nicholas.

Subjects/Keywords: Anthocyanidins; Oxidative stress; Antioxidant; Mitochondria

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APA (6^th Edition):

Peng, F. (2012). Mitochondrial uptake of anthocyanidins and protection from oxidative stress. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-08-626

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Peng, Fei. “Mitochondrial uptake of anthocyanidins and protection from oxidative stress.” 2012. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2012-08-626.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Peng, Fei. “Mitochondrial uptake of anthocyanidins and protection from oxidative stress.” 2012. Web. 05 Dec 2019.

Vancouver:

Peng F. Mitochondrial uptake of anthocyanidins and protection from oxidative stress. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2012-08-626.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Peng F. Mitochondrial uptake of anthocyanidins and protection from oxidative stress. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-08-626

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

2. Jantz, Nathan Timothy. Conjugated linoleic acid combined with creatine monohydrate and whey protein supplementation during strength training.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-11182009-112244

► The purpose of this thesis was to determine the combined effects of protein, creatine, and conjugated linoleic acid (CLA) supplementation during resistance training. These nutritional… (more)

Subjects/Keywords: nutrition; strength training; supplement; conjugated linoleic acid; CLA; whey protein; creatine

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APA (6^th Edition):

Jantz, N. T. (2009). Conjugated linoleic acid combined with creatine monohydrate and whey protein supplementation during strength training. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-11182009-112244

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jantz, Nathan Timothy. “Conjugated linoleic acid combined with creatine monohydrate and whey protein supplementation during strength training.” 2009. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/etd-11182009-112244.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jantz, Nathan Timothy. “Conjugated linoleic acid combined with creatine monohydrate and whey protein supplementation during strength training.” 2009. Web. 05 Dec 2019.

Vancouver:

Jantz NT. Conjugated linoleic acid combined with creatine monohydrate and whey protein supplementation during strength training. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/etd-11182009-112244.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jantz NT. Conjugated linoleic acid combined with creatine monohydrate and whey protein supplementation during strength training. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-11182009-112244

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

3. Rafiei, Hossein 1981-. Molecular mechanisms of protection by dietary polyphenols against free fatty acid-induced mitochondrial dysfunction and endoplasmic reticulum stress in an in vitro model of non-alcoholic fatty liver disease.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/7946

► Non-alcoholic fatty liver disease (NAFLD) is a public health burden. Steatosis as the “first hit”, and oxidative stress, inflammation, mitochondrial dysfunction, and endoplasmic reticulum stress… (more)

▼ Non-alcoholic fatty liver disease (NAFLD) is a public health burden. Steatosis as the “first hit”, and oxidative stress, inflammation, mitochondrial dysfunction, and endoplasmic reticulum stress as the “second hits” are the main contributors of the progression of fatty liver to non-alcoholic steatohepatitis (NASH). Dietary polyphenols have shown promise in protecting the liver against NAFLD. The relative effectiveness and mechanisms of different polyphenols however is mostly unknown. In this thesis HepG2 hepatocytes exposed to oleic or palmitic acid were used as a model system to explore the ability of selected polyphenols (resveratrol, quercetin, catechin, cyanidin, cyanidin-3-glucoside, berberine) from different classes to protect against molecular aspects of NAFLD and NASH. In an investigation of the “first hit” (Chapter 3), different polyphenols protected similarly against oleic acid-induced intracellular lipid accumulation, but differed in their effects on the expression of genes and proteins involved in lipogenesis, fatty acid oxidation, mitochondrial biogenesis, and bioenergetics. In an investigation of “second hits” (Chapter 4), most of the polyphenols decreased reactive oxygen species (ROS), prevented the decrease in uncoupling protein 2 (UCP2) mRNA, prevented the increase in tumor necrosis factor alpha (TNFα) mRNA, reversed decreases in mitochondrial biogenesis and increased expression of mitochondrial respiratory complexes and manganese superoxide dismutase (MnSOD). The anthocyanins were unique in decreasing ROS without inducing mitochondrial biogenesis or Mn-SOD mRNA expression. In investigations with palmitic acid (Chapter 5), exposure of HepG2 cells to palmitic acid induced endoplasmic reticulum (ER) stress evidenced by upregulated mRNA for the ER chaperones glucose-regulated protein 94 and 78 (GRP94, GRP78) and oxygen-regulated protein 150 (ORP150), cochaperone endoplasmic reticulum-localized DnaJ homologue 4 (ERdj4), and proapoptotic CCAAT-enhancer-binding protein homologous protein (CHOP). A few of the polyphenols (quercetin, catechin, cyanidin) protected against these changes. In a comparison of flavonoids with their phenolic breakdown/digestion products (Chapter 6), the polyphenols 2,4,6-trihydroxybenzaldehyde, protocatechuic acid, and caffeic acid protected similarly to quercetin and cyanidin against oleic and palmitic acid-induced steatosis and ROS generation. Moreover, in a short-term 1 h exposure (to limit spontaneous degradation in the medium), only breakdown/digestion products prevented an oleic acid-induced decrease of mitochondrial biogenesis. In conclusion, different classes of dietary polyphenols were all able to protect against steatosis and ROS generation in this in vitro model of NAFLD. Part of the mechanism for some polyphenols was through effects on mitochondrial biogenesis and function, bioenergetics, and ER stress. Phenolic breakdown/digestion products of flavonoids were shown to contribute to the protective effects of parent polyphenols. Advisors/Committee Members: Bandy, Brian, Arnason, Terra, Paterson, Phyllis, Krol, Edward, Zello, Gordon.

Subjects/Keywords: Non-alcoholic fatty liver disease; Mitochondrial dysfunction; endoplasmic reticulum stress; Steatosis

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APA (6^th Edition):

Rafiei, H. 1. (2017). Molecular mechanisms of protection by dietary polyphenols against free fatty acid-induced mitochondrial dysfunction and endoplasmic reticulum stress in an in vitro model of non-alcoholic fatty liver disease. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7946

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rafiei, Hossein 1981-. “Molecular mechanisms of protection by dietary polyphenols against free fatty acid-induced mitochondrial dysfunction and endoplasmic reticulum stress in an in vitro model of non-alcoholic fatty liver disease.” 2017. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/7946.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rafiei, Hossein 1981-. “Molecular mechanisms of protection by dietary polyphenols against free fatty acid-induced mitochondrial dysfunction and endoplasmic reticulum stress in an in vitro model of non-alcoholic fatty liver disease.” 2017. Web. 05 Dec 2019.

Vancouver:

Rafiei H1. Molecular mechanisms of protection by dietary polyphenols against free fatty acid-induced mitochondrial dysfunction and endoplasmic reticulum stress in an in vitro model of non-alcoholic fatty liver disease. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/7946.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rafiei H1. Molecular mechanisms of protection by dietary polyphenols against free fatty acid-induced mitochondrial dysfunction and endoplasmic reticulum stress in an in vitro model of non-alcoholic fatty liver disease. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7946

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

4. Thakur, Rajesh. GENDERED PERSPECTIVES ON FOOD INSECURITY IN SASKATOON.

Degree: 2016, University of Saskatchewan

URL: http://hdl.handle.net/10388/7432

► Food insecurity is a growing problem in Canada including Saskatoon. How gender is linked to household food insecurity is largely unexplored. Therefore, this study examined… (more)

▼ Food insecurity is a growing problem in Canada including Saskatoon. How gender is linked to household food insecurity is largely unexplored. Therefore, this study examined the relationship between gender and food insecurity based on the lived experience of 11 heterosexual couples seeking food assistance or living on social assistance in Saskatoon. This study assessed their perceptions, attitudes and beliefs about food security, household resource management, coping strategies, and food shopping and preparation practices. Data were collected by interviewing 11 couples and ten key informants and analyzed using Giorgi’s phenomenological approach. This study found food decision and grocery shopping were gendered. Female participants were involved more than their partners in decision-making about what food to buy and grocery shopping. Male participants viewed their partners more knowledgeable about food and shopping as feminine activity. Female participants felt more challenged than their spouses in grocery shopping and food preparation. There were no gender differences in other activities of household food management. Spouses supported each other and shared other household resources to manage food related activities. They held similar views about their household food situation and often agreed with each other about their household resources and the price, quality and type of food to buy. They bought foods that were affordable and nutritious. The food preferences of their family were accommodated where possible. They worked to ensure household food security. Food or money received from the Food Bank, CHEP and family were important in dealing with food insecurity. All participants and key informants agreed that food availability was not an issue but for some participants, affordability, access and time constraints were. Key informants and the participants suggested increasing support for families including more opportunities for income generation, increases in government welfare benefits, more grocery stores, transport assistance, and nutrition knowledge and cooking skill. The findings suggest policies related to gender as well as programs to improve food security in Saskatoon. Advisors/Committee Members: Berenbaum, Shawna, Bandy, Brian, Whiting, Susan, Vatanparast, Hassan, Engler-Stringer, Rachel.

Subjects/Keywords: Gender; Food Security; Food Insecurity; Phenomenology; Saskatoon; Canada

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thakur, R. (2016). GENDERED PERSPECTIVES ON FOOD INSECURITY IN SASKATOON. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7432

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thakur, Rajesh. “GENDERED PERSPECTIVES ON FOOD INSECURITY IN SASKATOON.” 2016. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/7432.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thakur, Rajesh. “GENDERED PERSPECTIVES ON FOOD INSECURITY IN SASKATOON.” 2016. Web. 05 Dec 2019.

Vancouver:

Thakur R. GENDERED PERSPECTIVES ON FOOD INSECURITY IN SASKATOON. [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/7432.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thakur R. GENDERED PERSPECTIVES ON FOOD INSECURITY IN SASKATOON. [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/7432

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

5. Chang, Chang. Encapsulation of omega fatty acid-rich oils using plant protein-based matrices.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/7745

► Oils rich in omega fatty acids (e.g., omega-3, -6, and -9) are both economically and nutritionally important to human beings, as they are playing significant… (more)

▼ Oils rich in omega fatty acids (e.g., omega-3, -6, and -9) are both economically and nutritionally important to human beings, as they are playing significant roles in the prevention of various diseases (e.g., coronary artery disease, hypertension, and diabetes) and maintenance of mental health. However, due to their unsaturated nature, susceptibility to oxidation, and immiscibility in aqueous products, microencapsulation was introduced to entrap the oils to circumvent these challenges. The overall goal of this thesis was to encapsulate omega fatty acid-rich oils (e.g., canola, fish, and flaxseed oils) using plant protein-based (e.g., pea, soy, lentil, and canola protein isolates) matrices, in order to enhance storage stability. In study one, the effect of pH (e.g., 3.0, 5.0, and 7.0) on the physicochemical (e.g., surface charge, hydrophobicity, and solubility), interfacial (e.g., interfacial tension and rheology), and emulsifying (e.g., droplet size and emulsion stability) properties of pea, soy, lentil, and canola protein isolates were determined to select one protein/pH to produce the most stable emulsion for encapsulation. Overall, proteins (at pH 7.0) with high surface charge, low hydrophobicity and high solubility showed a better ability to lower interfacial tension, whereas proteins (at pH 3.0) with high surface charge, hydrophobicity, and better solubility can form stronger viscoelastic films at the interface. All proteins could form stable emulsions away from their isoelectric point. Therefore, the selection of an effective plant protein emulsifier for encapsulation entails finding a balance between the properties needed to associate at the oil-water interface with those needed to develop a strong interfacial film. As such, lentil protein isolate (LPI) at pH 3.0 was selected as the most promising emulsifier to produce a stable emulsion, due to its high surface charge, solubility, and hydrophobicity. In study two, the LPI-based wall materials (e.g., maltodextrin, sodium alginate, and lecithin) were used to encapsulate canola oil (as a model oil) using spray drying, in order to design a microcapsule formulation, which offered good physical properties (e.g., moisture content, water activity, color, wettability, particle size, surface oil, and entrapment efficiency) and oxidative stability. Initially, mixtures of LPI (2-8%, w/w in initial emulsions) and maltodextrin (9.5-18%, w/w in initial emulsions) were used to entrap canola oil (20-30%, w/w in final microcapsules). Emulsion (e.g., emulsion stability, droplet size, viscosity) and microcapsule (e.g., surface oil and entrapment efficiency) properties were then characterized to determine a better capsule design. The microcapsules prepared with 20% oil, 2% LPI, and 18% maltodextrin were selected as a baseline to re-design better microcapsules using different preparation conditions and wall materials (e.g., sodium alginate and lecithin). Overall, the combination of LPI (2%), maltodextrin (17%), and sodium alginate (1%) presented the best capsule design to… Advisors/Committee Members: Nickerson, Michael, Low, Nicholas, Green, Rick, Bandy, Brian, Tanaka, Takuji.

Subjects/Keywords: Microencapsulation; omega fatty acid-rich oil; plant protein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chang, C. (2017). Encapsulation of omega fatty acid-rich oils using plant protein-based matrices. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7745

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chang, Chang. “Encapsulation of omega fatty acid-rich oils using plant protein-based matrices.” 2017. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/7745.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chang, Chang. “Encapsulation of omega fatty acid-rich oils using plant protein-based matrices.” 2017. Web. 05 Dec 2019.

Vancouver:

Chang C. Encapsulation of omega fatty acid-rich oils using plant protein-based matrices. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/7745.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chang C. Encapsulation of omega fatty acid-rich oils using plant protein-based matrices. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7745

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

6. Mirali, Mahla. Biochemical Profiling of Phenolic Compounds in Lentil Seeds.

Degree: 2016, University of Saskatchewan

URL: http://hdl.handle.net/10388/7444

► Lentil (Lens culinaris Medikus) is an annual cool-season legume with a variety of seed coat colours. Seed coat colour is an important grading factor that… (more)

▼ Lentil (Lens culinaris Medikus) is an annual cool-season legume with a variety of seed coat colours. Seed coat colour is an important grading factor that affects the market value of lentils. In lentil, two independent loci gray ground colour (Ggc) and tan ground colour (Tgc) determine the four basic seed coat background colours; brown (Ggc Tgc), gray (Ggc tgc), tan (ggc Tgc) and green (ggc tgc). The zero tannin locus (tan) is epistatic to the tgc locus, producing clear seed coats. Lentil is a good source of protein, carbohydrates, dietary fiber components, minerals, vitamins, and secondary metabolites that include phenolic compounds. Phenolic compounds produce different pigments in plants and bring health benefits to humans. The overall objective of this study was to determine the relationship between seed coat colour and phenolic compounds in lentil. In the first study, comparison of the phenolic profiles of four seed coat background colours in lentil (i.e., brown, gray, tan, and green) was performed using an optimized liquid chromatography-mass spectrometry (LC-MS) method. The results showed that for the levels of various phenolic compounds in lentil seeds varied with the seed coat colour. Specifically, seed coats of lentil genotypes carrying the homozygous recessive tgc allele (green and gray seed coats) had higher amounts of flavan-3-ols, proanthocyanidins, and some flavonols. In the second study, a comparison was made between the phenolic profiles of lentil seed coats that do not express the Tgc phenotype (genotype Tgc tan) and those that express Tgc (genotype Tgc Tan). The LC-MS analysis detected several compounds that were not influenced by tan, notably the phenolic acids, flavones, some flavonols, and some of dihydroflavonols. In contrast, myricetin, dihydromyricetin, and flavan-3-ols, and proanthocyanidin oligomers were detected only in Ggc Tgc Tan lines and therefore appear to be controlled by tan. The molecular analysis showed that tan is a basic-helixloop- helix (bHLH) transcription factor that could interact with the regulatory genes in the phenylpropanoid pathway for the enzymes flavonoid-3’,5’-hydroxylase (F3’5’H) and dihydroflavonol reductase (DFR). The third study measured the effect of long term storage on specific changes in phenolic compounds in lentil seeds. Increases in phenolic acids and flavones occur in green lentil seeds during storage, possibly because of the breakdown of more complex species into smaller subunits. More interestingly, a significant decrease in 27 flavan-3- ols and proanthocyanidins also occurs. Polymerization of flavan-3-ols and proanthocyanidins and their conjugation to cellular constituents could reduce their extractability and produce dark pigments in long stored lentil seeds. In conclusion, these studies determined that there is a relationship between phenolic compounds, specifically flavan-3-ols and proanthocyanidins, and seed coat colour genes tgc and tan in lentil. The findings of this study will help to develop future breeding strategies for lentil cultivars with… Advisors/Committee Members: Vandenberg, Albert, Purves, Randy, Bett, Kirstin, Hucl, Pierre, Bandy, Brian.

Subjects/Keywords: Lentil; Phenolic compounds; Seed coat colour; LC-MS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mirali, M. (2016). Biochemical Profiling of Phenolic Compounds in Lentil Seeds. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7444

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mirali, Mahla. “Biochemical Profiling of Phenolic Compounds in Lentil Seeds.” 2016. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/7444.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mirali, Mahla. “Biochemical Profiling of Phenolic Compounds in Lentil Seeds.” 2016. Web. 05 Dec 2019.

Vancouver:

Mirali M. Biochemical Profiling of Phenolic Compounds in Lentil Seeds. [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/7444.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mirali M. Biochemical Profiling of Phenolic Compounds in Lentil Seeds. [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/7444

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Viveky, Navita. Diet, Nutritional Status, Inflammation and Functional Outcomes in Older Adults Residing in Long Term Care Homes.

Degree: 2013, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2013-08-1161

► Many older adults residing in long term care (LTC) homes have underlying health conditions and limited functionality. The objective of this research was to address… (more)

▼ Many older adults residing in long term care (LTC) homes have underlying health conditions and limited functionality. The objective of this research was to address the issues which might affect the health status and functionality of older adults (age ≥ 60y) living in LTC homes. To achieve this objective, the research was carried out as four studies. In the first study, supplement and medication use was examined, specifically exploring the impact of dementia, and to assess pill burden in older adults residing in LTC home. In the second study, menu served in this LTC was assessed for recommendations of Canada’s Food Guide servings, macro, micro nutrients, and diet quality score. This menu analysis was compared to a similar analysis conducted a decade ago to find out what changes had occurred over time. In the third study, LTC residents were assessed for blood inflammatory markers, 25-hydroxyvitamin D (25(OH)D), metabolic syndrome, physical functioning, cognition, pain, and associations between inflammation and markers of functionality. Similar analyses on healthy community dwelling older and younger adults was conducted to draw differences amongst all three groups, i.e., older frail (LTC residents), older healthy, and younger healthy (community dwelling). In the fourth study the option of adding an anti-inflammatory diet was explored to address the issues of inflammation and compromised menus in LTC. Major findings suggested that there was inappropriate overuse of supplements, yet vitamin D supplements were consumed by only one-third residents of the LTC home. The LTC menu did not meet the recommendations for Canada’s Food Guide servings except for Fruits & Vegetables. Diet quality of the LTC menu was low and indicated the need of improvement, however, the comparison of current menu to a decade old menu showed some improvements. Inflammatory markers in LTC older adults were high, and the majority of subjects had insufficient 25(OH)D. Metabolic syndrome was seen in about more than one-third of subjects. Significant correlations between C-reactive protein, cognition and activities of daily living were detected. The anti-inflammatory diet was validated from research, and strategies of incorporating it into the diets of LTC residents were addressed. Advisors/Committee Members: Whiting, Susan, Alcorn, Jane, Thorpe, Lilian, Brawley, Larry, Bandy, Brian.

Subjects/Keywords: Long term care; older adults; nutrition and functional status

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APA (6^th Edition):

Viveky, N. (2013). Diet, Nutritional Status, Inflammation and Functional Outcomes in Older Adults Residing in Long Term Care Homes. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-08-1161

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Viveky, Navita. “Diet, Nutritional Status, Inflammation and Functional Outcomes in Older Adults Residing in Long Term Care Homes.” 2013. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2013-08-1161.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Viveky, Navita. “Diet, Nutritional Status, Inflammation and Functional Outcomes in Older Adults Residing in Long Term Care Homes.” 2013. Web. 05 Dec 2019.

Vancouver:

Viveky N. Diet, Nutritional Status, Inflammation and Functional Outcomes in Older Adults Residing in Long Term Care Homes. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2013-08-1161.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Viveky N. Diet, Nutritional Status, Inflammation and Functional Outcomes in Older Adults Residing in Long Term Care Homes. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-08-1161

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

8. O'Brien, Jaclyn Ann. A survey of methods to study zinc porphyrin aggregates in various media.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-08212010-090815

► Metalloporphyrin aggregation is critical for triplet-triplet annihilation (TTA) to occur. In order to maximize the efficiency of TTA, to use as a mechanism of photon… (more)

▼ Metalloporphyrin aggregation is critical for triplet-triplet annihilation (TTA) to occur. In order to maximize the efficiency of TTA, to use as a mechanism of photon upconversion in dye-sensitized solar cells, it is important to understand the phenomenon of absorber aggregation. The aggregation of ZnTPP in polymer films was investigated by fluorescence anisotropy and total internal reflection fluorescence microscopy (TIRFM). Single molecule spectroscopy (SMS) and spectromicroscopy were the techniques used to study single molecules and multimolecular aggregates of ZnTPP/ZnTPPS in polymer films/adsorbed on glass substrates. Fluorescence anisotropy measurements consistently showed depolarized emission from films most concentrated with ZnTPP. This observation was likely a result of energy transfer in and among porphyrin aggregates. Fluorescence intensity decays were also obtained and they illustrated a pattern of decreased fluorescence lifetime (i.e. faster decays) as the concentration of porphyrin in the film increased. These results are consistent with the formation of aggregates, and their increased presence in more concentrated films. The formation of these aggregates quenches the fluorophore’s fluorescence, resulting in the observed shorter fluorescence lifetimes. TIRFM was performed to study the structure of these polymer films doped with ZnTPP. It was determined that these films consisted of discrete domains and thus lacked homogeneity, and the presence of aggregates was clearly visible. Time-resolved TIRFM measurements were also performed but no interesting results were collected. SMS and spectromicroscopy were the final techniques employed to study porphyrin aggregation. Preliminary measurements were performed with polymer films doped with ZnTPP, and the single step decay time trajectories collected indicated that single molecules were being studied. Furthermore, emission spectra of these molecules were collected and they were similar to those obtained for a bulk measurement, but the bands were slightly shifted in comparison. These measurements were repeated with ZnTPPS adsorbed to glass substrates. Two different patterns of decay trajectories were measured: (i) single step decays corresponding to single ZnTPPS molecules and (ii) multi step/complex decays representative of multimolecular aggregates. Emission spectra were also collected for the multimolecular aggregates, and they were consistent with those of an ensemble measurement but slightly blue-shifted. Such a shift is common when studying aggregates on such a highly polar surface. Thus, these results demonstrate that ZnTPPS aggregates form even at concentrations as low as 10-8 M, and can be studied using SMS despite their weak fluorescence emission. Advisors/Committee Members: Paige, Matthew, Steer, Ronald P., Burgess, Ian, Bandy, Brian, Ward, Dale.

Subjects/Keywords: Single Molecule Spectroscopy; Aggregation; Triplet-Triplet Annihilation; Porphyrin

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APA (6^th Edition):

O'Brien, J. A. (2010). A survey of methods to study zinc porphyrin aggregates in various media. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08212010-090815

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

O'Brien, Jaclyn Ann. “A survey of methods to study zinc porphyrin aggregates in various media.” 2010. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/etd-08212010-090815.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

O'Brien, Jaclyn Ann. “A survey of methods to study zinc porphyrin aggregates in various media.” 2010. Web. 05 Dec 2019.

Vancouver:

O'Brien JA. A survey of methods to study zinc porphyrin aggregates in various media. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/etd-08212010-090815.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

O'Brien JA. A survey of methods to study zinc porphyrin aggregates in various media. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-08212010-090815

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

9. Kaban, Tyler. A strategy to develop prairie grapes (Vitis) with high trans-resveratrol production potential.

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2011-12-270

► A strategy to develop Prairie grapes (Vitis) with high trans-resveratrol production potential is proposed. Young greenhouse grown vines were induced to flower through training and… (more)

▼ A strategy to develop Prairie grapes (Vitis) with high trans-resveratrol production potential is proposed. Young greenhouse grown vines were induced to flower through training and pruning and were screened for resveratrol in ripe berry skins. The fourteen genotypes were selected based on percentage of V. riparia Michaux in their pedigrees. Based on reported percentage of riparia (0, ~25, 50 and 100%), four groups of genotypes were selected that had at least three genotypes per group. It was hypothesised that accessions with a higher percentage of riparia would produce more resveratrol and that pedigree could be used to predict production potential. The 50% V. riparia group included two additional accessions that were anthocyanin-deficient clones of ‘Frontenac’. Resveratrol production in grape skins was elicited with UVC light (254 nm) placed above and below detached berries. Incubation-day five was chosen as the day of highest observed resveratrol concentrations. All Vitis vinifera Linnaeus cultivars tested on this day were lower producers of trans-resveratrol than V. riparia selections. Of the cultivars tested, V. riparia x F1 hybrid ‘Valiant’ was the highest producer with an average of approximately 693 µg g-1 fresh weight. Pure V. riparia selection ‘DG Riparia’ was similar to ‘Valiant’ in its resveratrol production potential. The ‘gris’ and ‘blanc’ anthocyanin-deficient mutants of ‘Frontenac’ have similar capacity to produce resveratrol as the original cultivar. In conclusion, resveratrol production potential cannot be predicted purely on % V. riparia in pedigree but it was generally true that hybrids based on this species were higher producers than classic V. vinifera cultivars. Genotypes ‘DG Riparia’ and ‘Valiant’ will be useful as parents in breeding Prairie-adapted grapes high in trans-resveratrol. Greenhouse culture offers an effective means of early selection for resveratrol production potential through flower induction pruning. This induction protocol will also be a useful tool in breeding grapes at the University of Saskatchewan. Advisors/Committee Members: Bors, Bob, Coulman, Bruce, Bett, Kirstin, Bandy, Brian, Fisher, Hellen.

Subjects/Keywords: resveratrol; Vitis riparia; UVC; grape breeding

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APA (6^th Edition):

Kaban, T. (2011). A strategy to develop prairie grapes (Vitis) with high trans-resveratrol production potential. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2011-12-270

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kaban, Tyler. “A strategy to develop prairie grapes (Vitis) with high trans-resveratrol production potential.” 2011. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2011-12-270.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kaban, Tyler. “A strategy to develop prairie grapes (Vitis) with high trans-resveratrol production potential.” 2011. Web. 05 Dec 2019.

Vancouver:

Kaban T. A strategy to develop prairie grapes (Vitis) with high trans-resveratrol production potential. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2011-12-270.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kaban T. A strategy to develop prairie grapes (Vitis) with high trans-resveratrol production potential. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/ETD-2011-12-270

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

10. Ribeiro de Souza, Daniela 1992-. CHEMICAL COMPOSITION OF SELECT SASKATOON BERRY VARIETIES WITH AN EMPHASIS ON PHENOLICS.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/8086

► In this study, three saskatoon berry varieties (Martin, Northline and Pembina) grown in Saskatchewan, Canada were analyzed for their physicochemical properties (berry size, colour, pH… (more)

▼ In this study, three saskatoon berry varieties (Martin, Northline and Pembina) grown in Saskatchewan, Canada were analyzed for their physicochemical properties (berry size, colour, pH and % seeds), proximate composition, amino acids, major carbohydrates/polyols/galacturonic acid, major minerals, oligosaccharides, organic acids and phenolics. In addition, the phenolic subclass composition and antioxidant activities of whole fruit and pomace from commercial and laboratory scale juice production, and aqueous alcohol fractions were determined. Fruit varieties were found to differ in colour and size but showed similar pH and °Brix values. Proximate analysis results ranged from 80.18-82.79% for moisture, 7.39-10.82% for carbohydrate, 1.13-1.79% for protein, 0.28-0.48% for lipid, 4.23-9.42% for total dietary fibre, and 0.53-0.74% for ash. Major carbohydrates and polyol identified were fructose, glucose, and sorbitol. This work represents only the second report of the detection and quantitation of sorbitol in this fruit. Oligosaccharide profiles were determined by high performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) and capillary gas chromatography with flame ionization detection (CGC-FID) and showed the presence of a number of dextrose (DP2-5) and pectin polymers. Oligosaccharide profiles have not been reported previously. Amino acid contents ranged from 0.83-1.22 g/100 g fresh weight (FW), with arginine, aspartic acid/asparagine, glutamic acid/glutamine and leucine predominating. Major minerals quantified were calcium, magnesium, potassium and sodium, with potassium having the highest concentration that ranged from 219-248 mg/100 g FW. The major organic acids identified were malic (304.7-393.9 mg/100 g FW) and succinic (120.4-316.3 mg/100 g FW). Phenolics from the three fruit varieties were extracted employing water, ethanol:formic acid:water, and methanol:formic acid:water (70:2:28 v:v) mixtures. The ethanol:formic acid:water (EFW) extracts from all samples were found to have the highest phenolic concentrations as determined by total phenolic content analysis. Based on total phenolic chromatographic index (TPCI) results as determined by high performance liquid chromatography with photodiode array detection (HPLC-PDA), the Northline variety had the highest TPCI at 504.2 mg/100 g FW. This variety was also shown to have the highest antioxidant activities by both the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2´-azinobis-3-ethylbenzthiazoline-sulphonic acid (ABTS) radical scavenging assays, of 23.1 1/IC50/100 mg FW and 327.5 mM TEAC/100 mg FW, respectively. Solid phase extraction (SPE) using Amberlite® XAD16N resin and aqueous ethanol (40, 70 and 100%) was employed to produce phenolic fractions from the three fruit varieties. It was found that hydroxybenzoic acids eluted in the 40% ethanol fraction; hydroxycinnamic acids and anthocyanins eluted in the 70% ethanol fraction; and anthocyanins, flavanols and flavonols eluted in the 100% ethanol fraction. The 70% ethanol fraction had the… Advisors/Committee Members: Low, Nicholas H., Tanaka , Takuji, Green , Richard C., Bandy, Brian.

Subjects/Keywords: saskatoon berry (Amelanchier alnifolia Nutt.); chemical composition; pomace; phenolics

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APA (6^th Edition):

Ribeiro de Souza, D. 1. (2017). CHEMICAL COMPOSITION OF SELECT SASKATOON BERRY VARIETIES WITH AN EMPHASIS ON PHENOLICS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/8086

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ribeiro de Souza, Daniela 1992-. “CHEMICAL COMPOSITION OF SELECT SASKATOON BERRY VARIETIES WITH AN EMPHASIS ON PHENOLICS.” 2017. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/8086.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ribeiro de Souza, Daniela 1992-. “CHEMICAL COMPOSITION OF SELECT SASKATOON BERRY VARIETIES WITH AN EMPHASIS ON PHENOLICS.” 2017. Web. 05 Dec 2019.

Vancouver:

Ribeiro de Souza D1. CHEMICAL COMPOSITION OF SELECT SASKATOON BERRY VARIETIES WITH AN EMPHASIS ON PHENOLICS. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/8086.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ribeiro de Souza D1. CHEMICAL COMPOSITION OF SELECT SASKATOON BERRY VARIETIES WITH AN EMPHASIS ON PHENOLICS. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/8086

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

11. Tumback, Lindsay Nora Theresa. The Effect of Inulin Supplementation on the Quality of Life of Patients with Ileal Pouch Anal Anastomosis.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-04142009-211137

► Objectives: Ileal pouch anal anastomosis (IPAA), the removal of the colon and formation of a reservoir from ileum, is the surgery of choice for ulcerative… (more)

Subjects/Keywords: Ileal Pouch Anal Anastomosis; Inulin; Prebiotics; Pouchitis

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APA (6^th Edition):

Tumback, L. N. T. (2009). The Effect of Inulin Supplementation on the Quality of Life of Patients with Ileal Pouch Anal Anastomosis. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-04142009-211137

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tumback, Lindsay Nora Theresa. “The Effect of Inulin Supplementation on the Quality of Life of Patients with Ileal Pouch Anal Anastomosis.” 2009. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/etd-04142009-211137.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tumback, Lindsay Nora Theresa. “The Effect of Inulin Supplementation on the Quality of Life of Patients with Ileal Pouch Anal Anastomosis.” 2009. Web. 05 Dec 2019.

Vancouver:

Tumback LNT. The Effect of Inulin Supplementation on the Quality of Life of Patients with Ileal Pouch Anal Anastomosis. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/etd-04142009-211137.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tumback LNT. The Effect of Inulin Supplementation on the Quality of Life of Patients with Ileal Pouch Anal Anastomosis. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-04142009-211137

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

12. Alemmari, Abdulla. Role of Aluminum as a Toxic Element in Causing Parenteral Nutrition Associated Cholestasis.

Degree: 2014, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2014-02-1490

► Parenteral nutrition (PN) is an essential life sustaining therapy for premature and critically ill infants. However, prolonged PN therapy can lead to life-threatening liver damage,… (more)

▼ Parenteral nutrition (PN) is an essential life sustaining therapy for premature and critically ill infants. However, prolonged PN therapy can lead to life-threatening liver damage, and cause parenteral nutrition associated cholestasis (PNAC). There has been some recent evidence that aluminum accumulation in the livers of PN-fed subjects may lead to hepatic damage leading to liver injury. This dissertation aimed to investigate the role of aluminum as a toxic component of parenteral nutrition and as a risk factor in developing PNAC. The project composed of two main studies. The objectives of the first study were: 1) Evaluate the early morphological changes in piglet liver after intravenous administration of aluminum chloride hexahydrate at a dose of 1500 µg/kg/d.; 2) Determine whether the morphological changes deteriorate further with increasing duration of exposure and whether these changes correlate with changes in biochemical markers of cholestasis; 3) Identify the appropriate imaging technique for studying the ultrastructural changes in the liver; 4) Determine if intravenous injection of high dose aluminum into neonatal piglets disrupts iron homeostasis in the liver. The results showed that intravenous infusion of aluminum in neonatal piglets led to marked elevation in serum total bile acids, and transmission electron microscopy-energy dispersive microanalysis (TEM-EDX) was suitable in detecting the site of Al deposition in the liver and in demonstrating histopathological changes associated with Al infusion. The objectives of the second part were to: 1) Investigate the role of aluminum as a toxic component of parenteral nutrition and as a risk factor in causing liver injury; 2) Evaluate the effect of reducing aluminum content of parenteral nutrition on liver iron homeostasis; 3) Investigate the effect of low aluminum PN and high aluminum PN (regular PN) on the mRNA expression of Bsep and Mrp2. The results showed that administration of PN solution with lower Al content led to reduced levels of serum and hepatic Al in low Al PN group compared to regular PN group. This reduction was associated with less histopathological changes in the liver. On the other hand, administration of regular PN in piglets led to decreased expression of transporter Mrp2. This work suggests that reducing Al content in PN may reduce the development and severity of liver injury in the piglets. Advisors/Committee Members: Miller, Grant, Zello, Gordon, Arnold, Chris, Alcorn, Jane, Bandy, Brian, Moser, Mike.

Subjects/Keywords: Parenteral Nutrition; Cholestasis; Aluminum

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alemmari, A. (2014). Role of Aluminum as a Toxic Element in Causing Parenteral Nutrition Associated Cholestasis. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-02-1490

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alemmari, Abdulla. “Role of Aluminum as a Toxic Element in Causing Parenteral Nutrition Associated Cholestasis.” 2014. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2014-02-1490.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alemmari, Abdulla. “Role of Aluminum as a Toxic Element in Causing Parenteral Nutrition Associated Cholestasis.” 2014. Web. 05 Dec 2019.

Vancouver:

Alemmari A. Role of Aluminum as a Toxic Element in Causing Parenteral Nutrition Associated Cholestasis. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2014-02-1490.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alemmari A. Role of Aluminum as a Toxic Element in Causing Parenteral Nutrition Associated Cholestasis. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-02-1490

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

13. Almousa, Ahmed A. 1985-. Local effects of Linoorbitides and enterolactone On intestinal epithelial functions.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/7781

► The use of natural products is becoming increasingly popular worldwide. Flaxseed is a source of natural products that are rich with bioactives and have been… (more)

▼ The use of natural products is becoming increasingly popular worldwide. Flaxseed is a source of natural products that are rich with bioactives and have been reported to modulate inflammation, blood pressure, glucose levels and lipid profiles in both humans and animals. Currently, several known bioactives including fibers, lignans, alpha-linolenic acid, and linoorbitides (LOBs) are believed to impart those pharmacological activities. Previous studies have focused on the ability of flaxseed bioactives to provide their pharmacological action in the systemic circulation. However, our increasing understanding of their pharmacokinetics and chemical structures, suggests enhanced abundance of LOBs and lignans at the level of the intestinal epithelium due to the potential lack of permeation of LOBs, and susceptibility of lignans to enterohepatic circulation. Our aim in this work is to evaluate the effects of the lignan enterolactone (ENL) and LOBs on intestinal epithelium function and how it might influence associated pathologies like inflammatory bowel disease (IBD) and hypercholesterolemia. We first assessed LOBs and ENL effects on barrier integrity (a pathological hallmark of IBD) in vitro using a monoculture transwell system, and the potential mechanisms involved in maintenance of the barrier. Next, we examined their effect in a coculture system that more closely simulates the intact intestinal mucosa. These studies triggered the evaluation of pyruvate kinase M-2 (PKM2) as a possible biomarker of IBD. Lastly, we evaluated the effect of lignans on cholesterol metabolism in a human intestinal epithelial cell line (Caco-2). Our barrier integrity assessments of LOBs and ENL in the monoculture system identified differential effects of LOBs and ENL, and both showed increased protein levels of anti-inflammatory peroxisome proliferator-activated receptor gamma (PPAR-γ) and tight junction protein zonula, occludin (ZO-1). Further, in newly diagnosed IBD patients, PKM2 was indicative of disease presence with less variation than other biomarkers like fecal calprotectin and fecal lactoferrin. Lastly, altered cholesterol trafficking was observed in Caco-2 cells, an effect best attributed to the glucuronic acid conjugated form of enterolactone. These studies confirmed the importance of the flaxseed bioactives, ENL and LOBs in maintaining barrier integrity and modulation of cholesterol metabolism. More importantly, and due to their increased abundance at the level of the intestinal epithelium, those effects are crucially important in IBD and hypercholesterolemia. However, these effects remain to be evaluated in an animal models of colitis and dyslipidemia. Advisors/Committee Members: Alcron, Jane, Krol, Edward S., Blackburn, David, Bandy, Brian, Fowler, Sheryl, Muir, Alister.

Subjects/Keywords: Intestinal function; Enterolactone; Linoorbitides; Barrier Integrity; Cholesterol Trafficking

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Almousa, A. A. 1. (2017). Local effects of Linoorbitides and enterolactone On intestinal epithelial functions. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Almousa, Ahmed A 1985-. “Local effects of Linoorbitides and enterolactone On intestinal epithelial functions.” 2017. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/7781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Almousa, Ahmed A 1985-. “Local effects of Linoorbitides and enterolactone On intestinal epithelial functions.” 2017. Web. 05 Dec 2019.

Vancouver:

Almousa AA1. Local effects of Linoorbitides and enterolactone On intestinal epithelial functions. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/7781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Almousa AA1. Local effects of Linoorbitides and enterolactone On intestinal epithelial functions. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

14. Fahlman, Brian Micheal. In vitro studies to assess the potential of Quercetin as a topical sunscreen; photooxidative properties, photostability and inhibition of UV radiation-mediated skin damage.

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-01242011-115404

► Protection from the negative effects of solar radiation can be achieved by wearing protective clothing, avoiding exposure to sunlight or by the application of topical… (more)

▼ Protection from the negative effects of solar radiation can be achieved by wearing protective clothing, avoiding exposure to sunlight or by the application of topical sunscreens. In this thesis, a number of studies were designed to determine if quercetin is suitable for use as a topical sunscreen. The first objective was to determine if quercetin could protect against UV-induced lipid oxidation. Quercetin is twice as effective at preventing UVB-induced oxidation as preventing UVA-induced oxidation.The difference between UVA- and UVB- induced oxidation is believed to be due to the presence of an excited state form of quercetin in the UVA system. The second objective was to determine the UV photostability of quercetin in solution. Three photoproducts of quercetin form regardless of whether UVA or UVB radiation is used. These photoproducts are 2,4,6-trihydroxybenzaldehyde, quercetin depside and hydroxytyrosol. . The slow rate of formation, less than 20% loss of starting material over 11 hours, and non-toxic nature of the photoproducts indicate that photostability of quercetin is not an obstacle to its use as a sunscreen. The third objective was to determine the ability of quercetin to inhibit photosensitization by ketoprofen. Quercetin was shown to be effective in preventing decomposition of ketoprofen until it was consumed in the formation of the three quercetin photoproducts. This abilty of quercetin to prevent ketoprofen photosensitization indicates a beneficial effect for the use of quercetin as a topical sunscreen. The fourth objective was to determine if quercetin can prevent UV-induced damage in a biological system. Quercetin was found to significantly reduce secretion of matrix metalloprotease 1 (MMP-1) upon UVA or UVB exposure, but had no effect on secretion of tumor necrosis factor á (TNF-á) in HaCaT cells. , Topical application of quercetin to UVA or UVB exposed EpiDerm skin mimics significantly reduced both MMP-1 and TNF-á secretion. These results indicate that quercetin is effective in decreasing or eliminating several harmful effects of UVA and UVB radiation in the skin without major loss of starting material and without formation of toxic photoproducts. As such, quercetin appears to be a good candidate for inclusion into topical sunscreen formulations. Advisors/Committee Members: Krol, Ed S., Loppnow, Glen R., El-Aneed, Anas, Wilson, Ken, Alcorn, Jane, Bandy, Brian.

Subjects/Keywords: quercetin; natural products; sunscreens; photochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fahlman, B. M. (2011). In vitro studies to assess the potential of Quercetin as a topical sunscreen; photooxidative properties, photostability and inhibition of UV radiation-mediated skin damage. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-01242011-115404

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fahlman, Brian Micheal. “In vitro studies to assess the potential of Quercetin as a topical sunscreen; photooxidative properties, photostability and inhibition of UV radiation-mediated skin damage.” 2011. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/etd-01242011-115404.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fahlman, Brian Micheal. “In vitro studies to assess the potential of Quercetin as a topical sunscreen; photooxidative properties, photostability and inhibition of UV radiation-mediated skin damage.” 2011. Web. 05 Dec 2019.

Vancouver:

Fahlman BM. In vitro studies to assess the potential of Quercetin as a topical sunscreen; photooxidative properties, photostability and inhibition of UV radiation-mediated skin damage. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/etd-01242011-115404.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fahlman BM. In vitro studies to assess the potential of Quercetin as a topical sunscreen; photooxidative properties, photostability and inhibition of UV radiation-mediated skin damage. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/etd-01242011-115404

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

15. Asiamah, Isaac. Synthesis of nordihydroguaiaretic acid (NDGA) analogues and their oxidative metabolism.

Degree: 2015, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2015-06-1915

► Nordihydroguaiaretic acid (NDGA), is a naturally-occurring lignan isolated from the creosote bush (Larrea tridentata). The aqueous extract of this shrub, commonly referred to as Chaparral… (more)

▼ Nordihydroguaiaretic acid (NDGA), is a naturally-occurring lignan isolated from the creosote bush (Larrea tridentata). The aqueous extract of this shrub, commonly referred to as Chaparral tea, was listed in the American pharmacopeia as an ethnobotanical used to treat tuberculosis, arthritis and cancer. Other documented traditional applications of creosote bush extract include treatment for infertility, rheumatism, arthritis, diabetes, gallbladder and kidney stones, pain and inflammation among many others. In spite of the numerous pharmacological properties, NDGA use has been associated with toxicities including hepatotoxicity in humans. Previous studies in our group showed that oxidative cyclization of NDGA (a di-catechol) at physiological pH forms a dibenzocyclooctadiene that may have therapeutic benefits whilst oxidation to ortho-quinone likely mediates toxicological properties. In order to investigate the structural features responsible for pharmacological and toxicological properties, a series of NDGA analogues were designed, synthesized and characterized for the purpose of studying their oxidative metabolism. Literature procedures were modified to successfully prepare seven lignan analogues via multi-step synthesis. In our effort to understand the mechanisms of NDGA intramolecular cyclization, the prepared analogues were incubated under previously established conditions where NDGA autoxidized to yield the dibenzocyclooctadiene derivative. We also evaluated the stability of the analogues under the conditions of this study. Furthermore, we evaluated bioactivation potential of the prepared analogues with a goal of eliminating reactive metabolite liability through rational structural modification. We incubated NDGA and its analogues in rat liver microsomes (RLM) in the presence of glutathione as a nucleophilic trapping agent. Standards for comparison were generated by performing glutathione trapping experiments with chemical and enzyme oxidation systems. The potential of the dibenzocyclooctadiene lignan 2 derived from NDGA under physiological conditions to contribute to toxicological properties via reactive metabolite formation was also evaluated. Glutathione conjugates were detected by electrospray ionization-mass spectrometry (ESI-MS) scanning for neutral loss (NL) 129 Da or 307 Da in positive ion mode or precursor ion (PI) scanning for 272 Da in negative ion mode and further characterized by liquid chromatography–tandem mass spectrometry (LC–MS/MS) or in a single LC-MS run using multiple reactions monitoring (MRM) as a survey scan to trigger acquisition of enhanced product ion (EPI) data. We determined that NDGA autoxidation at pH 7.4 is dependent on substituents and/or substitution pattern on the two aromatic rings. In particular, spontaneous intramolecular cyclization to a dibenzocyclooctadiene required a di-catechol lignan, raising the possibility that o-Q formation may not be necessary for cyclization to occur. Cyclization was significantly inhibited in the presence of excess GSH which supports the… Advisors/Committee Members: Krol, Edward S., Remillard, Fred A., Palmer, David R., Bandy, Brian, Gravel, Michel.

Subjects/Keywords: nordihydroguaiaretic aci; lignans; dibenzocyclooctadiene; autoxidation; cyclization; bioactivation; reactive metabolites; glutathione adducts; liquid chromatography–tandem mass spectrometry (LC–MS/MS); multiple reactions monitoring (MRM); neutral loss (NL); precursor ion (PI).

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APA (6^th Edition):

Asiamah, I. (2015). Synthesis of nordihydroguaiaretic acid (NDGA) analogues and their oxidative metabolism. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-06-1915

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Asiamah, Isaac. “Synthesis of nordihydroguaiaretic acid (NDGA) analogues and their oxidative metabolism.” 2015. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2015-06-1915.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Asiamah, Isaac. “Synthesis of nordihydroguaiaretic acid (NDGA) analogues and their oxidative metabolism.” 2015. Web. 05 Dec 2019.

Vancouver:

Asiamah I. Synthesis of nordihydroguaiaretic acid (NDGA) analogues and their oxidative metabolism. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2015-06-1915.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Asiamah I. Synthesis of nordihydroguaiaretic acid (NDGA) analogues and their oxidative metabolism. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-06-1915

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

16. Duff, Whitney. The effects of novel anti-inflammatory nutritional and pharmaceutical supplementation during resistance training on muscle and bone in older adults.

Degree: 2015, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2015-12-2326

► Introduction: Chronic inflammation with aging is associated with sarcopenia and osteoporosis. Bovine colostrum is the first milk secreted by cows following parturition and contains bioactive… (more)

▼ Introduction: Chronic inflammation with aging is associated with sarcopenia and osteoporosis. Bovine colostrum is the first milk secreted by cows following parturition and contains bioactive substances, while ibuprofen is a non-steroidal anti-inflammatory drug. Both target the inflammatory pathway regulated by cyclooxygenase and have potential to increase muscle and bone mass when combined with resistance training. Objectives: To determine efficacy of novel anti-inflammatory nutritional (bovine colostrum) and pharmaceutical (ibuprofen) supplementation during resistance training on muscle and bone properties and strength in older adults. Methods: Older adults (≥50y) were randomly assigned to receive 38g/d of colostrum or whey protein during a resistance training program for 8 weeks; postmenopausal women (≥60y) were randomly assigned to receive ibuprofen (400 mg) or placebo post-exercise while performing a resistance training program or stretching program (3d/wk) for 9 months. Both studies utilized dual energy x-ray absorptiometry (DXA) for body composition and predicted 1-repetition maximum for strength. The bovine colostrum study further assessed muscle thickness of the biceps and quadriceps, plasma insulin-like growth factor-1, and inflammation and bone resorption markers; the ibuprofen study further assessed bone and muscle properties and estimates of bone strength (peripheral quantitative computed tomography), and dynamic balance. Results: Bovine colostrum supplementation during resistance training increased leg press strength (21%) and reduced bone resorption (-29%) versus whey protein. Both colostrum and whey protein groups improved chest press strength, muscle thickness, and lean tissue mass. Ibuprofen alone appeared beneficial for preventing loss of areal bone density at Ward’s region (3%) and bone properties at the distal radius (0.5%) and radial shaft (1.1%), while exercise alone appeared beneficial for bone properties at the distal radius (0.6%). However, the interaction of resistance training and ibuprofen negated the benefits at the distal radius (-1.5%). Neither ibuprofen nor resistance training was effective for increasing lean tissue mass, although resistance training improved body fat percentage (-2.0%), increased upper and lower body strength (23%, 110%), and preserved muscle density of the calf (-3.1%). Conclusion: While bovine colostrum could be taken within close proximity to exercise, ibuprofen should not be as it may interfere with the effects of exercise when the two interventions are combined. Advisors/Committee Members: Chilibeck, Philip D., Kontulainen, Saija A., Bandy, Brian, Baxter-Jones, Adam D., Zello, Gordon A..

Subjects/Keywords: Osteoporosis; sarcopenia; ibuprofen; bovine colostrum; resistance training

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APA (6^th Edition):

Duff, W. (2015). The effects of novel anti-inflammatory nutritional and pharmaceutical supplementation during resistance training on muscle and bone in older adults. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-12-2326

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Duff, Whitney. “The effects of novel anti-inflammatory nutritional and pharmaceutical supplementation during resistance training on muscle and bone in older adults.” 2015. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2015-12-2326.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Duff, Whitney. “The effects of novel anti-inflammatory nutritional and pharmaceutical supplementation during resistance training on muscle and bone in older adults.” 2015. Web. 05 Dec 2019.

Vancouver:

Duff W. The effects of novel anti-inflammatory nutritional and pharmaceutical supplementation during resistance training on muscle and bone in older adults. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2015-12-2326.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Duff W. The effects of novel anti-inflammatory nutritional and pharmaceutical supplementation during resistance training on muscle and bone in older adults. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-12-2326

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Smith, Shari. Effects of protein-energy malnutrition on the inflammatory response to global brain ischemia.

Degree: 2013, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2013-06-1076

► The overarching aim of the thesis research was to investigate mechanisms altered by protein-energy malnutrition (PEM), a common stroke co-morbidity factor that could affect the… (more)

▼ The overarching aim of the thesis research was to investigate mechanisms altered by protein-energy malnutrition (PEM), a common stroke co-morbidity factor that could affect the extent of brain damage and recovery following stroke. To model stroke, the rat 2-vessel occlusion model of global brain ischemia was employed. To characterize the effects of PEM, three states of malnutrition were assessed: PEM co-existing with brain ischemia (Study 1), effects of PEM independent of brain ischemia (Study 2), and PEM developing after brain ischemia (Study 3). The first hypothesis tested was co-existing PEM triggers an exacerbated glial response to global brain ischemia. The failure to achieve a consistent model of global ischemia prevented us from drawing conclusions on whether co-existing PEM exacerbates reactive gliosis. Nonetheless, this study demonstrated that mean temperature and temperature fluctuation are increased within the first 24hr of exposure to a low protein diet. The second hypothesis tested was PEM causes sustained changes in core temperature that are associated with an inflammatory response. Exposure to a low protein diet caused an immediate small and transient increase in mean temperature and a larger sustained increase in temperature amplitude. As malnutrition evolved, mean temperature declined. PEM stimulated an acute-phase response, characterized by an increase in the positive acute-phase protein, alpha-2-macroglobulin (A2M), and a decrease in the negative acute-phase protein, albumin. This response appeared to be aberrant, since the positive acute-phase protein, alpha-1-acid glycoprotein (AGP), was decreased with PEM. The final hypothesis tested was PEM developing after global brain ischemia exacerbates systemic and hippocampal inflammation, which is associated with diminished neuroplasticity. The effects of PEM on the acute-phase response are persistent following brain ischemia, as demonstrated by decreased serum albumin and increased serum A2M. A decrease in the positive acute-phase protein, haptoglobin, strengthened the evidence that PEM triggers an atypical reaction. The strong glial response elicited by global ischemia was unaltered by PEM. However, PEM influenced hippocampal neuroplasticity mechanisms, as GAP-43 and synaptophysin were significantly lower at d21. In summary, it has been demonstrated that PEM affects core temperature, the systemic acute-phase reaction and the neuroplasticity response to global brain ischemia. Advisors/Committee Members: Paterson, Phyllis G., Nazarali, Adil, Bandy, Brian, Singh, Baljit, Colbourne, Fred, Tuor, Ursula.

Subjects/Keywords: protein-energy malnutrition; inflammation; global brain ischemia; temperature; acute-phase response; neuroplasticity

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APA (6^th Edition):

Smith, S. (2013). Effects of protein-energy malnutrition on the inflammatory response to global brain ischemia. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-06-1076

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Smith, Shari. “Effects of protein-energy malnutrition on the inflammatory response to global brain ischemia.” 2013. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2013-06-1076.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Smith, Shari. “Effects of protein-energy malnutrition on the inflammatory response to global brain ischemia.” 2013. Web. 05 Dec 2019.

Vancouver:

Smith S. Effects of protein-energy malnutrition on the inflammatory response to global brain ischemia. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2013-06-1076.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Smith S. Effects of protein-energy malnutrition on the inflammatory response to global brain ischemia. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-06-1076

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

18. Dawson, James. Concentration and Content of Secondary Metabolites.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/7819

► The University of Saskatchewan (UofS) has been conducting crosses of Lonicera caerulea and releasing genotypes for fruit production under the name “Haskap”. The primary objectives… (more)

▼ The University of Saskatchewan (UofS) has been conducting crosses of Lonicera caerulea and releasing genotypes for fruit production under the name “Haskap”. The primary objectives of the UofS Haskap Breeding Program are to improve fruit flavor, increase fruit size and facilitate mechanical harvest. A more recent additional objective is to increase the concentration and content of compounds with the potential to enhance human health in the fruit and leaves of new haskap genotypes.As a first step to meet this additional objective, this project surveyed the secondary metabolites present within fruit and leaves of haskap. Genotypes tested included genotypes released by the UofS Haskap Breeding Program, unnamed genotypes with potential for use in breeding programs, genotypes acquired from germplasm repositories as well as genotypes of Lonicera caerulea subsp. villosa. Secondary metabolites were selected for further study if they were both linked to human health and were found at sufficient concentrations in haskap to allow for quantification. Chlorogenic acid, quercetin (three glycosides), loganin and secologanin matched these selection criteria. HPLC and mass spectrometry methodologies were developed to allow for quantification and identification of the target secondary metabolites in methanolic extracts of haskap fruit and leaves. Concentrations of the selected secondary metabolites decreased with fruit development, but the overall content (concentration x fruit weight) increased. In fruit tested at harvest maturity, the highest concentrations of many compounds of interest occurred in the widely grown cultivar Tundra. The concentrations of secondary metabolites in haskap leaves also decreased over the growing season, however at the end of the season, substantial amounts of secondary metabolites were still present in the leaves. The fruit and leaves of Lonicera caerulea subsp. villosa germplasm had a different quercetin profile than the other Lonicera caerulea genotypes surveyed. The concentrations of secondary metabolites in the fruit of the various genotypes were negatively correlated with the individual fruit weight produced by each genotype. The concentrations of some of the metabolites in fruit and leaves of Lonicera caerulea subsp. villosa also varied with the geographic site of origin of the genotypes. Post-harvest treatment of haskap fruit with UVC did not enhance the secondary metabolite profile. Advisors/Committee Members: Waterer, Doug, Bors, Bob, Tanino, Karen, Gray, Gord, Bandy, Brian, Bai, Yuguang.

Subjects/Keywords: Lonicera caerulea Secondary metabolite Haskap Fruit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dawson, J. (2017). Concentration and Content of Secondary Metabolites. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7819

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dawson, James. “Concentration and Content of Secondary Metabolites.” 2017. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/7819.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dawson, James. “Concentration and Content of Secondary Metabolites.” 2017. Web. 05 Dec 2019.

Vancouver:

Dawson J. Concentration and Content of Secondary Metabolites. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/7819.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dawson J. Concentration and Content of Secondary Metabolites. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7819

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

19. Cammer, Allison Lee 1975-. NUTRITION CARE FOR LONG-TERM CARE RESIDENTS WITH DEMENTIA IN URBAN AND RURAL CONTEXTS: AN EVIDENCE BASED PRACTICE EXAMINATION OF THE ROLE OF CARE AIDES AND REGISTERED DIETITIANS.

Degree: 2018, University of Saskatchewan

URL: http://hdl.handle.net/10388/8636

► This doctoral dissertation contributes to the body of knowledge pertaining to nutrition care for persons with dementia. The aging population adds to the increased risk… (more)

▼ This doctoral dissertation contributes to the body of knowledge pertaining to nutrition care for persons with dementia. The aging population adds to the increased risk for and prevalence of dementia globally. Alongside this is a recognition of the need for care strategies for persons with dementia, and preventive strategies to delay onset of dementia or to delay secondary or tertiary comorbidity associated with dementia. Of strong interest is the field of nutrition, in terms of dietary strategies for primary prevention of dementia, for secondary and tertiary prevention of comorbidities, and for medical nutrition therapies to treat those with dementia, across the spectrum of dementing illnesses and degree of severity. Over the course of three related studies, nutrition care for long-term care (LTC residents was addressed using an evidence-based practice lens. Study 1 investigated care aides’ perception of nutrition care for urban and rural LTC residents with dementia. Key findings included the complexity of operationalizing person centered care into nutrition care activities, the mechanistic focus on feeding, and many organizational factors that direct nutrition care for care aides and residents with dementia. Study 2 examined the role of registered dietitians (RDs) in providing nutrition care for residents with dementia in urban and rural LTC. The key finding in this study was the downstream role of RDs in dementia care. As opposed to a more upstream preventive model of care, RDs were consulted or involved only at late stages or when comorbid decline had occurred, limiting their abilities to use their specialized nutrition knowledge and skills effectively. The 3rd study was an umbrella review of the peer-reviewed body of systematic reviews on nutrition care for residents with dementia in LTC. The major findings of this study include the lack of consistency in terms of nutritional outcomes considered and intervention tested, as well as a considerable gap in the published literature regarding both care aides and RDs. Taken together, these studies make a valuable contribution to the growing body of research on nutrition care and dementia. There is a need to continue to work with RDs and care aides in developing and testing interventions that can enhance both the physical health and quality of life for LTC residents with dementia. Advisors/Committee Members: Whiting, Susan, Morgan, Debra, Goodridge, Donna, Paterson, Phyllis, Dobson, Roy, Bandy, Brian.

Subjects/Keywords: Nutrition; Dementia; Long-term care; Care Aide; Dietitian

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APA (6^th Edition):

Cammer, A. L. 1. (2018). NUTRITION CARE FOR LONG-TERM CARE RESIDENTS WITH DEMENTIA IN URBAN AND RURAL CONTEXTS: AN EVIDENCE BASED PRACTICE EXAMINATION OF THE ROLE OF CARE AIDES AND REGISTERED DIETITIANS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/8636

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cammer, Allison Lee 1975-. “NUTRITION CARE FOR LONG-TERM CARE RESIDENTS WITH DEMENTIA IN URBAN AND RURAL CONTEXTS: AN EVIDENCE BASED PRACTICE EXAMINATION OF THE ROLE OF CARE AIDES AND REGISTERED DIETITIANS.” 2018. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/8636.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cammer, Allison Lee 1975-. “NUTRITION CARE FOR LONG-TERM CARE RESIDENTS WITH DEMENTIA IN URBAN AND RURAL CONTEXTS: AN EVIDENCE BASED PRACTICE EXAMINATION OF THE ROLE OF CARE AIDES AND REGISTERED DIETITIANS.” 2018. Web. 05 Dec 2019.

Vancouver:

Cammer AL1. NUTRITION CARE FOR LONG-TERM CARE RESIDENTS WITH DEMENTIA IN URBAN AND RURAL CONTEXTS: AN EVIDENCE BASED PRACTICE EXAMINATION OF THE ROLE OF CARE AIDES AND REGISTERED DIETITIANS. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/8636.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cammer AL1. NUTRITION CARE FOR LONG-TERM CARE RESIDENTS WITH DEMENTIA IN URBAN AND RURAL CONTEXTS: AN EVIDENCE BASED PRACTICE EXAMINATION OF THE ROLE OF CARE AIDES AND REGISTERED DIETITIANS. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/8636

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

20. Billinsky, Jennifer Lynn. Oxidative metabolism and cytochrome P450 enzyme inhibition potential of creosote bush and flaxseed lignans.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-09212009-165345

► The rising use of natural products creates an imperative need for an enhanced awareness of the safety of current and new products making their way… (more)

▼ The rising use of natural products creates an imperative need for an enhanced awareness of the safety of current and new products making their way into the marketplace. An important example is natural products containing lignans as the principal active component. Despite their structural similarity the lignan of creosote bush can cause hepato- and renal toxicity while the lignans of flaxseed have no reported serious toxicity. This dissertation aimed to investigate the oxidative metabolism of such lignans to determine whether reversible, competitive interactions and/or bioactivation may explain the differences in their apparent toxicity. The first objective was to study the metabolism and bioactivation of nordihydroguaiaretic acid (creosote bush) and secoisolariciresinol (flaxseed). Nordihydroguaiaretic acid metabolism in rat liver microsomes led to the production of three glutathione adducts formed via ortho¬-quinone reactive intermediates. This metabolism was independent of NADPH and thus attributed to autoxidation. Secoisolariciresinol metabolism yielded lariciresinol and no glutathione adducts suggesting an absence of bioactivation to reactive quinone intermediates. The second objective was to study the autoxidation of nordihydroguaiaretic acid. The major autoxidation product was a unique, stable schisandrin-like cyclolignan which was the result of nordihydroguaiaretic acid cyclization. The half-life of nordihydroguaiaretic acid in aqueous solution, pH 7.4, 37ºC is 3.14 hours suggesting the cyclolignan may be responsible for some of the biological effects of nordihydroguaiaretic acid. The third objective was to study the inhibition of cytochrome P450 isoforms 1A2, 2B, 2C11 and 3A by lignans derived from creosote bush and flaxseed. None of the lignans caused irreversible inhibition. Both creosote bush and flaxseed lignans caused reversible inhibition of P450 enzyme activity that involved competitive or mixed-type inhibition, however the inhibition was present at nonphysiologically relevant concentrations. Activation of cytochrome P450 isoforms was also observed at low lignan concentrations. The results suggest that P450-mediated bioactivation or reversible inhibition cannot explain the differences in toxicity noted between the lignans of creosote bush and flaxseed. This work suggests a minimal risk for drug-lignan interactions at P450 enzymes. Further studies are warranted to determine the presence and biological and toxicological role of the nordihydroguaiaretic acid cyclolignan in herbal preparations. Advisors/Committee Members: Krol, Ed S., Blakley, Barry R., Alcorn, Jane, Bandy, Brian, Janz, David M., Siraki, Arno.

Subjects/Keywords: Secoisolariciresinol; Nordihydroguaiaretic acid; Flaxseed; quinone; Creosote bush; Anhydrosecoisolariciresinol; Enterolactone; Enterodiol; Dibenzocyclooctadiene; Cytochrome P450; bioactivation; inhibition; reactive intermediate; lignan; hepatotoxicity; autoxidation; Glutathione adduct

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APA (6^th Edition):

Billinsky, J. L. (2009). Oxidative metabolism and cytochrome P450 enzyme inhibition potential of creosote bush and flaxseed lignans. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-09212009-165345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Billinsky, Jennifer Lynn. “Oxidative metabolism and cytochrome P450 enzyme inhibition potential of creosote bush and flaxseed lignans.” 2009. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/etd-09212009-165345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Billinsky, Jennifer Lynn. “Oxidative metabolism and cytochrome P450 enzyme inhibition potential of creosote bush and flaxseed lignans.” 2009. Web. 05 Dec 2019.

Vancouver:

Billinsky JL. Oxidative metabolism and cytochrome P450 enzyme inhibition potential of creosote bush and flaxseed lignans. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/etd-09212009-165345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Billinsky JL. Oxidative metabolism and cytochrome P450 enzyme inhibition potential of creosote bush and flaxseed lignans. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-09212009-165345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

21. Di, Yunyun 1985-. Flaxseed Lignan Supplementation as Possible Adjuvant Therapy for Prostate and Breast Cancer.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/7679

► Dietary flaxseed lignans may have a chemopreventive and chemotherapeutic role against prostate and breast cancers. Flaxseed lignans, though, undergo an extensive first-pass effect and exist… (more)

▼ Dietary flaxseed lignans may have a chemopreventive and chemotherapeutic role against prostate and breast cancers. Flaxseed lignans, though, undergo an extensive first-pass effect and exist systemically primarily as glucuronide conjugates that are believed to be inactive. Their poor oral bioavailability (FO) likely explains the very modest benefit observed in human clinical studies. To fully realize the potential for lignan oral supplementation in prostate and breast cancer, pharmaceutical strategies are necessary to overcome the limitations imposed by poor FO. This dissertation involved proof-of-principle studies designed to first provide in vitro experimental evidence of flaxseed lignans on enhancing cytotoxicity of typical chemotherapeutic agents when used in combination, followed by the development of a pharmaceutical strategy to potentially exploit a role for lignans as adjuvant therapy against prostate cancer, and a human clinical trial to show oral safety and tolerability. Combination studies of lignans with chemotherapeutic agents were first conducted in prostate (PC3 and LNCap) and breast (SKBR3 and MDA-MB-231) cancer cell lines. SECO, the aglycone metabolite of the plant lignan, secoisolariciresinol diglucoside (SDG), was tested as it exhibits good FO but has had little investigation. As a major mammalian lignan converted from SECO, studies focused on enterolactone (ENL) and its glucuronic acid conjugate (ENL-Gluc). Typical chemotherapeutic agents with different mechanisms of action, including docetaxel, doxorubicin, cabazitaxel, MDV3100, and carboplatin were selected for combination experiments with lignans. A randomized, double-blind, placebo-controlled clinical trial in healthy older adults was conducted with oral supplementation of a standard flaxseed lignan-enriched complex (~38% SDG) equivalent to 600 mg SDG for 6-months, to assess efficacy, safety, and tolerability of flaxseed lignans. Finally, antibody directed enzyme prodrug therapy (ADEPT) system was generated by using anti-prostate specific membrane antigen (PSMA) antibody, D7 scFv as the carrier, and human β-glucuronidase (hβG) as the drug-converting enzyme. The binding affinity of fusion protein was assessed in purified PSMA as well as LNCap cells expressing cell surface PSMA. The enzymatic activity of D7-hβG fusion protein was determined using probe, 4-methylumberlliferone glucuronide, and prodrug, ENL-Gluc. C4-2 cells, expressing PSMA, were chosen to measure the conversion of ENL-Gluc into ENL by fusion protein in combination with docetaxel. In the in vitro combination studies, SECO and ENL enhanced sensitivity of cancer cells against therapeutic agents, in particular the ENL and docetaxel combination, while no obvious cytotoxicity was observed from ENL-Gluc. The in vivo assessment of flaxseed lignan-enriched product indicated no adverse side effects suggesting the safety and tolerability of flaxseed lignans for long-term oral exposure at a low pharmacological dose. Furthermore, plasma parent and total flaxseed lignans were significantly… Advisors/Committee Members: Alcorn, Jane, Krol, Ed, Dobson, Roy, Yang, Jian, Bandy, Brian, Harkness, Troy.

Subjects/Keywords: Flaxseed lignans; Adjuvant therapy; ADEPT

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APA (6^th Edition):

Di, Y. 1. (2017). Flaxseed Lignan Supplementation as Possible Adjuvant Therapy for Prostate and Breast Cancer. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7679

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Di, Yunyun 1985-. “Flaxseed Lignan Supplementation as Possible Adjuvant Therapy for Prostate and Breast Cancer.” 2017. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/7679.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Di, Yunyun 1985-. “Flaxseed Lignan Supplementation as Possible Adjuvant Therapy for Prostate and Breast Cancer.” 2017. Web. 05 Dec 2019.

Vancouver:

Di Y1. Flaxseed Lignan Supplementation as Possible Adjuvant Therapy for Prostate and Breast Cancer. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/7679.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Di Y1. Flaxseed Lignan Supplementation as Possible Adjuvant Therapy for Prostate and Breast Cancer. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7679

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

22. Yunus, Fakir Md. Feasibility of field implementation of fortified lentils to improve iron status status of adolescent girls in Bangladesh.

Degree: 2018, University of Saskatchewan

URL: http://hdl.handle.net/10388/8363

► The purpose of this crossover study was to establish methodology and logistics to conduct future large-scale community-based efficacy studies. A crossover trial was carried out… (more)

▼ The purpose of this crossover study was to establish methodology and logistics to conduct future large-scale community-based efficacy studies. A crossover trial was carried out in which adolescent girls consumed fortified lentil consisting of 2 different cooking preparation styles (thick vs thin) and 3 different lentil ‘portion size’ of raw weight 25 g, 37.5 g and 50 g. The cooked lentils were served 5 days a week over 12 weeks mixed with a standard cup (250 mL) of cooked rice among 100 adolescent girls. Small cotyledon lentil (iron 75-90 ppm) were fortified using NaFeEDTA iron fortificant solution with 1600 ppm of Fe at the Crop Development Centre (CDC), University of Saskatchewan. Visual Analog Scales (VAS) were used to measure the rating of hunger, satiety, and palatability before the serving of the meal, after one bite of the meal and after finishing the meal. In-depth interviews and focus group discussions were carried out with the participants’ mother/guardian to evaluate acceptability and lentil preparation styles at the end of the trial. Mean (SD) age and age of menarche of the adolescent girls were 12.85 (2.00) and 12.06 (0.94) years, respectively. Fifty-four percent heard about iron deficiency anemia, and 48% were of the opinion that eating more iron-rich foods could prevent anemia. The results showed that thick preparation of cooked lentil at 37.5 g portion size had higher positive scores in all VAS variables compared to the thin preparation. Considering the raw amount served, the thick preparation of lentil at 37.5 g would provide 6.9 mg Fe/d. This would cover approximately 86% (and 66% who have started to menstruate) and 46% of the RDA for adolescent girls aged 9-13 years and 14-18 years, respectively. A thick preparation of cooked lentil at 200 g (37.5 g raw lentil amount) would be preferable for a fortified lentil intervention in future human efficacy trials. Advisors/Committee Members: Zello, Gordon, DellaValle, Diane M., Bandy, Brian, Nickerson, Michael, Jalal, Chowdhury, Warkentin, Tom.

Subjects/Keywords: Fortification; Iron; Adolescents; Iron Deficiency Anemia (IDA); Community-based trial; Lentils; Dietary Intake; Study Design.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yunus, F. M. (2018). Feasibility of field implementation of fortified lentils to improve iron status status of adolescent girls in Bangladesh. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/8363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yunus, Fakir Md. “Feasibility of field implementation of fortified lentils to improve iron status status of adolescent girls in Bangladesh.” 2018. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/8363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yunus, Fakir Md. “Feasibility of field implementation of fortified lentils to improve iron status status of adolescent girls in Bangladesh.” 2018. Web. 05 Dec 2019.

Vancouver:

Yunus FM. Feasibility of field implementation of fortified lentils to improve iron status status of adolescent girls in Bangladesh. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/8363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yunus FM. Feasibility of field implementation of fortified lentils to improve iron status status of adolescent girls in Bangladesh. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/8363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

23. Bagonluri, Mukasa Tenyogtaa. Evaluation of the purity and dispersion of single walled carbon nanotubes as potential pharmaceutical excipients.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-05072010-143911

► Single walled carbon nanotubes (SWNTs) are considered potential biomedical materials because of their flexible structure, hollow interior for fluidic transport, propensity for functionalization of the… (more)

▼ Single walled carbon nanotubes (SWNTs) are considered potential biomedical materials because of their flexible structure, hollow interior for fluidic transport, propensity for functionalization of the exterior walls, and biocompatibility. Research into exploiting these properties has focused on SWNTs as building blocks for novel drug-delivery systems, dosage forms, and biomedical substrates. However, the use of the internal nanochannels as conduits for trans-membrane drug delivery has not been explored. This research was initially designed to explore the latter. It is postulated that due to their mechanical strength and the presence of an internal conduit, SWNTs can be used for nanofluidic transport. Using a magnetic field, the magnetically responsive SWNT are driven into intact stratum corneum, creating nanochannels, for trans-membrane drug delivery. Initial studies showed however that a bottleneck is the aggregation of SWNTs on the surface of stratum corneum. To achieve trans-membrane nanofluidic delivery, the SWNTs have to be well dispersed in an appropriate pharmaceutical medium, and the SWNT have to be of high purity. Similarly, the presence of impurities in SWNTs, and the dispersion state of these materials in pharmaceutical solvents may give an insight into the discrepancies in toxicity that is reported. The purity of five commercially available SWNTs (AP-SWNT and P2-SWNT, from Carbon Solutions Inc, HMS-SWNT from Helix Materials, and NA-SWNT from Nanostructured and Amorphous Materials Inc. and CT-SWNT from ChepTubes Inc.) were analyzed by raman and electron dispersive x-ray spectroscopy (EDS) spectroscopy. Secondly, the dispersion states of SWNTs in various pharmaceutical solvents were evaluated by ultraviolet (UV) spectroscopy, scanning electron microscopy (SEM), dynamic light scattering (DLS), zeta potential, and Raman spectroscopy to identify potential agents for exfoliation of SWNTs in selected pharmaceutical solution. SWNTs were dispersed in various solvents (water, propylene glycol [PG], dimethylsulfoxide [DMSO], and ethanol) as well as in 0.1% w/v aqueous solutions of anionic, cationic and neutral surfactants at a SWNT concentration of 0.1 mg/mL. SWNT suspensions described as dispersed yielded an evenly coloured suspension with no visible precipitate. The most stable dispersions were obtained with the gemini surfactants, which were confirmed by SEM observation of exfoliated SWNTs. Zeta (î) potential measurements of the fully dispersed SWNTs showed typical values of greater than +30 mV, while non-dispersed samples were less than +20 mV. SEM images of the dispersed solution showed the presence of exfoliated SWNTs compared to the aggregated SWNT clusters observed in non-dispersed systems. Raman spectra of dispersed SWNTs showed G-band peak shifts (to higher wavelengths), confirming the presence of exfoliated SWNTs. Even though the purity of SWNT did not correlate with amount of SWNT in dispersion, exfoliation of bundled SWNTs was accompanied by an increase in UV absorbance of the dispersion, with… Advisors/Committee Members: Foldvari, Marianna, Wilson, Lee, Bandy, Brian, Sammynaiken, R, Alcorn, Jane, Nazarali, Adil.

Subjects/Keywords: excipients; dispersion; purity of single walled carbon nanotubes; Single walled carbon nanotubes

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APA (6^th Edition):

Bagonluri, M. T. (2010). Evaluation of the purity and dispersion of single walled carbon nanotubes as potential pharmaceutical excipients. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-05072010-143911

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bagonluri, Mukasa Tenyogtaa. “Evaluation of the purity and dispersion of single walled carbon nanotubes as potential pharmaceutical excipients.” 2010. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/etd-05072010-143911.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bagonluri, Mukasa Tenyogtaa. “Evaluation of the purity and dispersion of single walled carbon nanotubes as potential pharmaceutical excipients.” 2010. Web. 05 Dec 2019.

Vancouver:

Bagonluri MT. Evaluation of the purity and dispersion of single walled carbon nanotubes as potential pharmaceutical excipients. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/etd-05072010-143911.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bagonluri MT. Evaluation of the purity and dispersion of single walled carbon nanotubes as potential pharmaceutical excipients. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-05072010-143911

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

24. Addala, Eshwari. In vitro studies using curcumin and curcumin analogues as candidate mitochondria-targeting anticancer agents affecting colon cancer cells.

Degree: 2014, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2014-09-1714

► Curcumin is one of the major curcuminoids produced by the ginger family Zingiberaceae. These curcuminoids possess pharmacological properties that include anticancer activities. We have evaluated… (more)

▼ Curcumin is one of the major curcuminoids produced by the ginger family Zingiberaceae. These curcuminoids possess pharmacological properties that include anticancer activities. We have evaluated some synthetic curcumin analogues that have shown potential as anticancer drugs. These antineoplastic agents bearing the 1,5-diaryl-3-oxo-1,4-pentadienyl pharmacophore are electrophiles which are designed to preferentially react with sulfhydryl groups present in proteins as opposed to amino and hydroxyl groups present in DNA. In previous pilot studies, three derivatives examined in this thesis showed inhibition towards human cancer cell lines such as Molt 4/C8 and CEM T-lymphocytes. In this thesis work, I determined the cytotoxicity of these derivatives and curcumin towards human colon cancer (HCT-116) cells and also normal colon epithelial (CRL-1790) cells, and examined the possible mechanism(s) involved. I hypothesized that they act via induction of reactive oxygen species (ROS) which elicit a transient surge of mitochondrial ROS generation and a phenomenon known as ROS-induced ROS release (RIRR), along with the mitochondrial permeability transition (MPT) and mitochondrion –dependent apoptosis. I asked whether these agents react with some of the key protein thiols in the mitochondria whose oxidation/alkylation results in mitochondrion - dependent apoptosis. NC-2109 and NC-2346 were found to be potent cytotoxic agents based on their GI50 values of 0.87 ± 0.38 μM and 0.90 ± 0.22 μM, respectively, and were more potent than the anticancer drug 5-fluorouracil (GI50 = 5.47 ± 0.55 μM) and curcumin (GI50 = 3.50 ± 0.36 μM). However NC-2109 was found to have a better selectivity towards cancer cells over normal cells (a selectivity index of 18.81 versus 5-FU, curcumin and NC-2346 which had selectivity indices of 1.87, 16.75 and 4.61, respectively). In the investigations of the mechanisms involved, both curcumin and curcumin analogues were able to induce mitochondrial ROS production. Moreover, curcumin and its synthetic counterparts showed a biphasic ROS profile which is most characteristic of RIRR. Treatment with these agents also led to the disruption of the mitochondrial membrane potential, suggesting oxidation of protein thiols and the opening of the mitochondrial permeability transition pore which is an important step to initiate mitochondria-directed apoptosis. This possibility was confirmed based on GSSG/GSH ratios, since curcumin, NC-2346 and NC-2109 all produced a higher GSSG/GSH ratio than the controls. In addition to their ability to depolarize the mitochondrial membrane in HCT-116 cells, that these molecules acted via the mitochondrial pathway were further authenticated based on their ability to induce mitochondrial swelling in rat liver mitochondria. In another part of this thesis I evaluated the involvement of the critical thiol protein adenine nucleotide translocase (ANT), a bifunctional protein that plays a central role in mitochondrial apoptosis. ANT has four different isoforms; ANT1 and ANT3 are proapoptotic,… Advisors/Committee Members: Bandy, Brian J., Dimmock, Jonathan R., Paterson, Phyllis, Badea, Ildiko, Krol, Ed, Sharma, Rajendra.

Subjects/Keywords: apoptosis; mitochondria; reactive oxygen species; curcumin; anticancer; unsaturated ketones

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Addala, E. (2014). In vitro studies using curcumin and curcumin analogues as candidate mitochondria-targeting anticancer agents affecting colon cancer cells. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-09-1714

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Addala, Eshwari. “In vitro studies using curcumin and curcumin analogues as candidate mitochondria-targeting anticancer agents affecting colon cancer cells.” 2014. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2014-09-1714.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Addala, Eshwari. “In vitro studies using curcumin and curcumin analogues as candidate mitochondria-targeting anticancer agents affecting colon cancer cells.” 2014. Web. 05 Dec 2019.

Vancouver:

Addala E. In vitro studies using curcumin and curcumin analogues as candidate mitochondria-targeting anticancer agents affecting colon cancer cells. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2014-09-1714.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Addala E. In vitro studies using curcumin and curcumin analogues as candidate mitochondria-targeting anticancer agents affecting colon cancer cells. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-09-1714

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

25. Jazeri, Rana 1988-. Macro and micronutrient content of foods served to 3-5-year-old children before and after pulse intervention and factors influencing the sustainability of pulse-based foods in Saskatoon childcare centres.

Degree: 2019, University of Saskatchewan

URL: http://hdl.handle.net/10388/12232

► This study examined pre and post-intervention macro- and micronutrient content of regular and pulse-based intervention meals served to 3-5-year old (yo) children at four Saskatoon… (more)

▼ This study examined pre and post-intervention macro- and micronutrient content of regular and pulse-based intervention meals served to 3-5-year old (yo) children at four Saskatoon childcare centres (CCs); and factors, which are perceived as important for the adoption, implementation, and continuation of a pulse-based nutrition education intervention at CCs. The Pulse Discovery Tool Kit (PDTK) was an intervention introduced into four CCs in Saskatoon in order to promote pulse-based foods. In the present multi-methods study: quantitative analysis of macronutrient and micronutrient content of thirteen regular menu items and four pulse-based intervention recipes was carried out; as well as an analysis of plate waste data obtained previously that measured food preferences of 3-5 yo children. The following macro- and micronutrients were measured: calories, carbohydrates, fats, saturated fats, proteins, sodium, total fibre, calcium, folate, iron, magnesium, potassium, zinc and the vitamins A and C. The results for the macro- and micronutrients were calculated for 100g of each type of food. In addition, qualitative data was obtained from school staff one year following to determine the sustainability of the PDTK intervention. Results showed that pulse-based foods provided fewer calories, similar amounts of protein and carbohydrates, higher fiber, lower fat and unsaturated fats, and similar amounts of micronutrients such as calcium, iron, magnesium, potassium, sodium, zinc, folate and the vitamins C and A. With respect to plate waste, it varied from a low of 5.4% for beef tacos and a high of 78% for ranch dip. Generally, the plate waste was higher for the pulse-based foods as compared to the regular foods. This indicated that the children did not like the pulse-based foods, perhaps because they were not familiar with pulses and due to a reluctance to try a novel food. The staff interviews revealed six themes: knowledge of the CC food guidelines; length of time it took to cook the recipes; the effort in preparing the pulse recipes; price of the pulses; fatiguing the children with too many pulse-based foods; and the difficulty in having the kids eat the PDTK recipes. It was found that the staff were generally aware of the provincial and federal guidelines for foods served to children at CCs. However, all of the cooks, and some directors, felt that the pulse-based food recipes were time-consuming and too complex to make. Price was not seen as a factor in the serving of pulses in CCs. However, all of the staff were wary of fatiguing the children with too many pulse foods and noted that the children, on the whole, did not like eating the pulse foods. The reasons for this, according to the staff, were due to pulses being novel and the texture of the pulse-based foods. Although the cooks at the CCs used small amounts of pulses in some of their foods, they did not make any of the pulse foods that were made during the PDTK intervention. Overall, this study revealed that pulses are an excellent plant-based alternative to meats and… Advisors/Committee Members: Henry, Carol, Whiting, Susan [email protected], Bandy, Brian, Vatanparast, Hassan, Spurr, Shelley.

Subjects/Keywords: macro and micronutrient content; pulse intervention

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jazeri, R. 1. (2019). Macro and micronutrient content of foods served to 3-5-year-old children before and after pulse intervention and factors influencing the sustainability of pulse-based foods in Saskatoon childcare centres. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12232

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jazeri, Rana 1988-. “Macro and micronutrient content of foods served to 3-5-year-old children before and after pulse intervention and factors influencing the sustainability of pulse-based foods in Saskatoon childcare centres.” 2019. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/12232.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jazeri, Rana 1988-. “Macro and micronutrient content of foods served to 3-5-year-old children before and after pulse intervention and factors influencing the sustainability of pulse-based foods in Saskatoon childcare centres.” 2019. Web. 05 Dec 2019.

Vancouver:

Jazeri R1. Macro and micronutrient content of foods served to 3-5-year-old children before and after pulse intervention and factors influencing the sustainability of pulse-based foods in Saskatoon childcare centres. [Internet] [Thesis]. University of Saskatchewan; 2019. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/12232.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jazeri R1. Macro and micronutrient content of foods served to 3-5-year-old children before and after pulse intervention and factors influencing the sustainability of pulse-based foods in Saskatoon childcare centres. [Thesis]. University of Saskatchewan; 2019. Available from: http://hdl.handle.net/10388/12232

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

26. Abeysekara, Saman. D-lactic acid metabolism and control of acidosis.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-01202009-120329

► D-lactic acidosis (DLA) is a disease associated with D-lactatemia, acidosis and neurological signs. However, these associations are ill-defined. Bacterial fermentation in the intestine causes increasing… (more)

▼ D-lactic acidosis (DLA) is a disease associated with D-lactatemia, acidosis and neurological signs. However, these associations are ill-defined. Bacterial fermentation in the intestine causes increasing D-lactic acid concentrations in the body. Therefore, DLA is reported secondary to gastrointestinal diseases, such as short bowel syndrome, gastroenteritis or diarrhea. Despite intestinal origin, sudden D-lactatemia is often a result of impaired D-lactate metabolism in the body. Aims of this work were to determine: 1) Influence of the presence of D-lactate or acidity on neurological disturbances; 2) Effectiveness of parenteral NaHCO3 therapy in correcting cerebrospinal acidity and DLA; 3) Prevalence of DLA in diarrheic lambs and fecal D-lactate thresholds; 4) Effectiveness of malate in preventing DLA. The methodological tools consisted of animal models (calves and lambs): 1) Advanced surgical procedure in calves for long-lasting atlanto-occipital catheterizations; 2) Intravenous infusions of acids to experimentally induce acidosis; 3) Intravenous NaHCO3 therapies; 4) Sampling of cerebrospinal fluid (CSF), blood, urine and feces from experimental / treated calves or diarrheic lambs for blood gas analysis, and D-lactate separation by chromatography. D-lactate entered the central nervous system (> 2 mmol/L) from the circulation following experimentally induced D-lactatemia (> 5 mmol/L) and was responsible for neurological disturbances which correlated (r = 0.9, P < 0.05) with both CSF and serum D-lactate concentrations. A zenith of neurological disturbances, ataxia was evident when D-lactate concentration exceeded 12 mmol/L (CSF) and 26 mmol/L (serum), however, a nadir of acidosis (pH 6.9) caused by HCl infusions produced only mild neurological disturbances (P < 0.05). Therapeutic NaHCO3 infusions did not result paradoxical CSF acidosis, but supportive in correcting (P < 0.05) acidosis (ÄpH + 0.11) and D-lactatemia in calves. In lambs, metabolic acidosis following a range of mild to severe diarrhea was observed with a corresponding range of D-lactate concentrations in both serum (< 0.05−24.0 mmol/L) and feces (< 0.05−31.0 mmol/L). D-lactate was absorbed into the circulation when the fecal D-lactate concentration exceeded 10.2 mmol/L (threshold). In calves, moderate oral use of malate produced a > 50% (P < 0.05) decrease in fecal and serum D-lactate concentrations suggesting prebiotic properties to prevent DLA. This dissertation answers the critical questions about the onset of neurological signs in D-lactic acidosis, and advances the current knowledge on the metabolism of D-lactate, the prevention and treatment of acidosis. Advisors/Committee Members: Naylor, J. M., Zello, Gordon A., Paterson, Phyllis G., Hamilton, Donald L., Chilibeck, Philip D., Brocks, D. R., Bandy, Brian.

Subjects/Keywords: D-lactatic acidosis; Incidence; Treatment; Prebiotic; Neuropathy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abeysekara, S. (2009). D-lactic acid metabolism and control of acidosis. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-01202009-120329

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abeysekara, Saman. “D-lactic acid metabolism and control of acidosis.” 2009. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/etd-01202009-120329.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abeysekara, Saman. “D-lactic acid metabolism and control of acidosis.” 2009. Web. 05 Dec 2019.

Vancouver:

Abeysekara S. D-lactic acid metabolism and control of acidosis. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/etd-01202009-120329.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abeysekara S. D-lactic acid metabolism and control of acidosis. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-01202009-120329

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Abou Hadeed, Ahmed. The protective effect of ascorbate and catechin against myocardial ischemia-reperfusion injury in an isolated rat heart model.

Degree: 2014, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2014-09-1782

► Myocardial ischemia-reperfusion (I/R) injury is an important health concern in myocardial infarction and situations such as angioplasty and cardiac surgeries. Therefore, patients and physicians need… (more)

▼ Myocardial ischemia-reperfusion (I/R) injury is an important health concern in myocardial infarction and situations such as angioplasty and cardiac surgeries. Therefore, patients and physicians need therapeutic interventions that are applicable at the time of surgery. Flavonoids and ascorbate (vitamin C) are known for their antioxidant activity and may be involved in the currently known health benefits of plant based foods and drinks. The objectives of this study were to 1) determine the extent to which ascorbate or catechin alone at levels which could be in blood after dietary supplementation, can protect myocardial tissue in the reperfusion phase of I/R injury, and 2) evaluate the possible cooperative or synergistic protective effect of ascorbate and catechin when given together. Isolated rat hearts (n=48) were perfused in the retrograde mode with modified Krebs-Henseleit buffer, and following the induction of 30 min global ischemia, ascorbate (150 µM) and/or catechin (5 µM) were added directly into the perfusate during 90 min reperfusion. To determine the histopathological features, hematoxylin and eosin (H&E) stain was used in one heart per condition; while to assess the biochemical analysis, the heart tissues were assessed for apoptosis (caspase-3 activity), oxidative stress (thiobarbituric acid reactive substances (TBARS) and total malondialdehyde (MDA) levels), and redox status (reduced and oxidized glutathione tissue levels). A comparison of IR hearts with two controls, sham (perfused for a 15 min stabilization period) and continuous perfusion (perfused for 135 min), showed in most but not all measurements that this was a suitable model of IR injury. The treatment experiments showed that 150 µM ascorbate protected the heart against lipid peroxidation and cell apoptosis by 100%, while 5 µM catechin protected by 67% and 90% respectively. No cooperative protective effect could be observed when ascorbate and catechin were used together. None of the treatments significantly affected either reduced or oxidized glutathione levels. In conclusion, this study showed strong protection by ascorbate, which could be used in clinically relevant situations, and is the first to report the protection by catechin at this dose under conditions of myocardial ischemia-reperfusion injury. Advisors/Committee Members: Bandy, Brian, Paterson, Phyllis, Lee, Paul.

Subjects/Keywords: ischemia-reperfusion injury; ascorbate; catechin

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APA (6^th Edition):

Abou Hadeed, A. (2014). The protective effect of ascorbate and catechin against myocardial ischemia-reperfusion injury in an isolated rat heart model. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-09-1782

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abou Hadeed, Ahmed. “The protective effect of ascorbate and catechin against myocardial ischemia-reperfusion injury in an isolated rat heart model.” 2014. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2014-09-1782.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abou Hadeed, Ahmed. “The protective effect of ascorbate and catechin against myocardial ischemia-reperfusion injury in an isolated rat heart model.” 2014. Web. 05 Dec 2019.

Vancouver:

Abou Hadeed A. The protective effect of ascorbate and catechin against myocardial ischemia-reperfusion injury in an isolated rat heart model. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2014-09-1782.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abou Hadeed A. The protective effect of ascorbate and catechin against myocardial ischemia-reperfusion injury in an isolated rat heart model. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-09-1782

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. GAO, BOSONG. INTERACTIONS BETWEEN FLAVONOIDS, VITAMIN C AND VITAMIN E IN PROTECTING MEMBRANES AGAINST OXIDATIVE STRESS.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-06-478

► min C, as a water-soluble antioxidant and vitamin E being the major lipid-soluble antioxidant, play vital roles in preventing oxidative damage. Flavonoids are a class… (more)

▼ min C, as a water-soluble antioxidant and vitamin E being the major lipid-soluble antioxidant, play vital roles in preventing oxidative damage. Flavonoids are a class of plant secondary metabolites well known for antioxidant activities that due to their amphiphilicity may interact with both vitamin C and vitamin E. This research investigates their cooperation with vitamin C and vitamin E on oxidative status in vitro using artificial liposomes and isolated rat liver mitochondrial membranes. Vitamin C induced pro-oxidant effects in both systems and the oxidative stress was positively correlated with its concentration. The presence of vitamin E in liposomes inhibited the pro-oxidant effect of ascorbic acid, and the flavonoid quercetin inhibited this effect in vitamin E-free liposomes. Experiments with added tert-butyl hydroperoxide were used to test for a possible cooperation between vitamin C, flavonoids, and vitamin E in inhibiting lipid peroxidation. While most of the conditions did not reveal cooperation, evidence for cooperation involving quercetin was observed in liposomes at the lowest level of vitamin E tested. In measurements of vitamin E (α-tocopherol) content in mitochondria using HPLC and mass spectrometry, the combination of vitamin C and quercetin was able to synergistically regenerate the α-tocopherol in the presence of hydroperoxide while the regeneration did not happen with either vitamin C or quercetin alone. However, the recovery of vitamin E did not relate to the TBARS measurement, indicating that the α-tocopherol concentration was not directly correlated to the TBARS formation. Based on this research, acorbate has pro-oxidative effects on both systems, which can be suppressed by quercetin but not completely by vitamin E. Among the tested flavonoids, only quercetin was observed to synergistically interact with acorbate to regenerate α- tocopherol, although under this condition the interaction did not rescue the mitochondria from oxidative damage. Advisors/Committee Members: Bandy, Brian, Paterson, Phyllis G., Krol, Ed S., Wanasundara, Janitha.

Subjects/Keywords: Vitamin C; Vitamin E; flavonoids; oxidative stress; synergism

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APA (6^th Edition):

GAO, B. (2012). INTERACTIONS BETWEEN FLAVONOIDS, VITAMIN C AND VITAMIN E IN PROTECTING MEMBRANES AGAINST OXIDATIVE STRESS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-06-478

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

GAO, BOSONG. “INTERACTIONS BETWEEN FLAVONOIDS, VITAMIN C AND VITAMIN E IN PROTECTING MEMBRANES AGAINST OXIDATIVE STRESS.” 2012. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2012-06-478.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

GAO, BOSONG. “INTERACTIONS BETWEEN FLAVONOIDS, VITAMIN C AND VITAMIN E IN PROTECTING MEMBRANES AGAINST OXIDATIVE STRESS.” 2012. Web. 05 Dec 2019.

Vancouver:

GAO B. INTERACTIONS BETWEEN FLAVONOIDS, VITAMIN C AND VITAMIN E IN PROTECTING MEMBRANES AGAINST OXIDATIVE STRESS. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2012-06-478.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

GAO B. INTERACTIONS BETWEEN FLAVONOIDS, VITAMIN C AND VITAMIN E IN PROTECTING MEMBRANES AGAINST OXIDATIVE STRESS. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-06-478

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Andrade Ramos, Rafaela. Modelling the protein-energy malnourished stroke patient.

Degree: 2013, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2013-06-1073

► Little is known about the effects of protein-energy malnutrition (PEM) developing after stroke on brain recovery. The goal of this project was to develop two… (more)

▼ Little is known about the effects of protein-energy malnutrition (PEM) developing after stroke on brain recovery. The goal of this project was to develop two experimental models in the adult rat to allow evaluation of nutritional effects on post-stroke recovery: (1) a PEM model, and (2) a photothrombotic stroke model. Experiment 1 examined the hypothesis that a diet containing either 1% or 0.5% protein will produce an acute state of mild-moderate PEM in adult rats. Male, Sprague-Dawley rats (16 wk) were trained in the Montoya staircase before being randomized to diets containing 0.5% (n=8), 1% (n=8), or 12.5% protein (n=10 [CON]) for 31d. Both low protein diets increased liver lipid content (p< 0.001) and decreased food intake (p= 0.005) and body weight (p< 0.001) compared to the 12.5% protein diet. The 0.5% protein group best mimicked the stroke patient, as judged by decreased serum albumin (p= 0.018) and an acute decrease in mean (±SEM) body weight (g) by d7 (0.5%= 424±15; 1%= 428±14; CON= 477±10; p = 0.011). Increased concentrations of the positive acute phase proteins, alpha-2-macroglobulin and alpha-1-acid glycoprotein, were greatest in the 0.5% group (p< 0.001). No differences were observed in the Montoya test on d3, 15, or 30 (p= 0.26). Values on d30 were: 0.5%= 109.5±4.4% of pre-diet performance; 1%= 97.2±5.5%; CON= 98.5±10.2%. Experiment 2 tested the hypothesis that targeted laser irradiation and 30 mg/kg of rose Bengal injection will cause an infarct in the forepaw region of the cortex with accompanying functional deficits. Male adult rats trained in the Montoya staircase were randomized to ISCHEMIA (n=15) or SHAM (n=3) surgery. A cortical infarct occurred in 86% of rats, with some misplacement and variability in volume (5.7-12.8 mm3). Forepaw impairments were confirmed by decreased performance in the staircase at d3 (34.3±7.3 % of pre-stroke performance, p<0.001) and diminished use in the cylinder test (30.3±4.0% affected limb use versus 53.9±1.93% prestroke, p< 0.001). At d30, mean recovery was incomplete in the staircase (p< 0.001). These experimental models, with additional refinements, can be used to address the hypothesis that deteriorating nutritional status after a stroke interferes with brain recovery. Advisors/Committee Members: Paterson, Phyllis G., Muir, Gillian, Whiting, Susan, Bandy, Brian.

Subjects/Keywords: Protein-energy malnutrition; rat models; photothrombotic stroke.

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APA (6^th Edition):

Andrade Ramos, R. (2013). Modelling the protein-energy malnourished stroke patient. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-06-1073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Andrade Ramos, Rafaela. “Modelling the protein-energy malnourished stroke patient.” 2013. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2013-06-1073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Andrade Ramos, Rafaela. “Modelling the protein-energy malnourished stroke patient.” 2013. Web. 05 Dec 2019.

Vancouver:

Andrade Ramos R. Modelling the protein-energy malnourished stroke patient. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2013-06-1073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Andrade Ramos R. Modelling the protein-energy malnourished stroke patient. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-06-1073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Selinger, Martina. Dietetic scope of practice.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-11-772

► Scope of practice is seen as the base from which governing bodies prepare standards of practice, educational institutions organize curricula and employers prepare job descriptions.… (more)

▼ Scope of practice is seen as the base from which governing bodies prepare standards of practice, educational institutions organize curricula and employers prepare job descriptions. The meaning of scope of practice varies among and within healthcare professions. The purpose of this study was to explore the concept of scope of practice for dietetics in Saskatchewan and Canada. Little is known about dietetic scope of practice in Canada and literature has not shown a consistent use of the term. Saskatchewan is one of two provincial dietetic regulatory bodies without a scope of practice in legislation. Implications for having an undefined scope of practice include role confusion, competition among providers, diminishing professional identity and under- or over-utilization of health professionals. Using interpretative description methodology, data was collected through four phases. In Phase I, eight provincial dietetic regulatory bodies participated in semi structured telephone interviews on scope of practice. Phase II used an online survey on 92 Saskatchewan dietitians to examine dietetic scope of practice. A follow up online focus group was conducted with ten Saskatchewan dietitians in Phase III to further explore dietetic scope of practice. Phase IV related dietetic scope of practice statements found across Canada to literature and data collected in Phases I-III. Themes were defined as a topic identified by participants in more than one phase. Data from all phases were coded using NVivo 9.0. Results indicate that dietetic scope of practice statements are a product of a complex multi-player, political process. Without a scope of practice, Saskatchewan dietitian participants were interpreting their role from a combination of documents (e.g. ethics, research) and stakeholders (e.g. employers and colleagues). Participants believed a dietetic scope of practice should provide guidance to dietitians, employers, health professionals, the public, regulatory bodies, and other stakeholders. Challenges defining, understanding and working with a scope of practice include encompassing the diversity of dietetics in a concise statement, and working with stakeholders who have a poor understanding of the dietetic profession. Our research also revealed variation in dietetic scopes of practice across Canada. A scope of practice cycle was proposed which included four phases: no scope of practice, creating a scope of practice, using a scope of practice and evaluating and updating a scope of practice. Factors influencing a scope of practice, such as the political environment, education of professionals and employer policies all impact this scope of practice cycle. Perceived outcomes of a scope of practice include guidance, credibility, advanced practice and practice protection. Advisors/Committee Members: Berenbaum, Shawna, Suveges, Linda, Ferguson, Linda, Bandy, Brian.

Subjects/Keywords: Scope of Practice; Dietitian; Professional Practice; dietetics; education; professional standards

…health sciences curricula in Canadian universities, including the University of Saskatchewan… …Since 1999, the University of Saskatchewan has organized interprofessional problem based… …University of Saskatchewan facilitates other interprofessional opportunities for students such as…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Selinger, M. (2012). Dietetic scope of practice. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-11-772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Selinger, Martina. “Dietetic scope of practice.” 2012. Thesis, University of Saskatchewan. Accessed December 05, 2019. http://hdl.handle.net/10388/ETD-2012-11-772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Selinger, Martina. “Dietetic scope of practice.” 2012. Web. 05 Dec 2019.

Vancouver:

Selinger M. Dietetic scope of practice. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2019 Dec 05]. Available from: http://hdl.handle.net/10388/ETD-2012-11-772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Selinger M. Dietetic scope of practice. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-11-772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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