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You searched for +publisher:"University of Saskatchewan" +contributor:("Asai-Coakwell, Mika"). Showing records 1 – 2 of 2 total matches.

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University of Saskatchewan

1. Ketel, Crystal R. Investigating Scur Candidate Genes in Bos taurus cattle.

Degree: 2020, University of Saskatchewan

Scurs are loose horns that are inherited in a sex-influenced manner and appear in cattle that are heterozygous (Pp) for the polled mutation. Beef producers find them undesirable, but they are difficult to eradicate because of a complex inheritance. The aims of this study were: 1) to confirm the polled/horned genotype in scurred families from a Canadian beef research herd (CBRH), scurred cattle families from producers, and feedlot steers using the Celtic poll test (PC) and Friesen poll test (PF), and 2) to identify new candidate genes near microsatellite BMS2142 on BTA19. Through PCR amplification, the PC genotype was confirmed in the phenotyped CBRH, Simmental and Blonde D’Aquitaine (BA) scurred families, and in 625 feedlot steers. One hundred and forty nine scurred animals (out of 692) had one PC allele. PCR amplification revealed that the PF allele was present in four polled steers that were horned using the PC test. Five scur candidate genes (CTDNEP1, FGF11, SOX15, SHBG, and DHRS7C) were chosen based on position and function on BTA19. To identify SNPs segregating with scurs, 16 animals were chosen from the PC genotyped feedlot steers, 8 Pp scurred steers and 8 Pp polled steers. Two SNP’s found in CTDNEP1 and DHRS7C were examined in the CBRH and BA with PCR-RFLP using BseRI and AciI, respectively, but did not segregate with scurs. Multipoint analysis calculated by CRI-MAP 2.5.4, determined that there was significant linkage of the scur locus to two microsatellites on BTA19 (BMS2142 LOD=5.42; IDVGA42 LOD=3.47). In conclusion, this study’s fine mapping of the scur locus has increased the LOD scores of surrounding loci and was linked to two microsatellites on BTA19. Also, to identify scurs the animals should be carefully phenotyped and genotyped for PC, using the PF for inconsistent results in beef breeds. Advisors/Committee Members: Asai-Coakwell, Mika, Buchanan, Fiona, Brook, Ryan, Lardner, Bart, Campbell, John, Roesler, Bill.

Subjects/Keywords: Scurred; polled; horned; beef cattle

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ketel, C. R. (2020). Investigating Scur Candidate Genes in Bos taurus cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12793

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ketel, Crystal R. “Investigating Scur Candidate Genes in Bos taurus cattle.” 2020. Thesis, University of Saskatchewan. Accessed March 04, 2021. http://hdl.handle.net/10388/12793.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ketel, Crystal R. “Investigating Scur Candidate Genes in Bos taurus cattle.” 2020. Web. 04 Mar 2021.

Vancouver:

Ketel CR. Investigating Scur Candidate Genes in Bos taurus cattle. [Internet] [Thesis]. University of Saskatchewan; 2020. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/10388/12793.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ketel CR. Investigating Scur Candidate Genes in Bos taurus cattle. [Thesis]. University of Saskatchewan; 2020. Available from: http://hdl.handle.net/10388/12793

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of Saskatchewan

2. Hamilton, Kelsey Evelyn 1994-. A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle.

Degree: 2019, University of Saskatchewan

min A (VA) has a nutrigenomic effect on intramuscular fat. Discovering variants in genes involved in fat deposition that are also affected by vitamin A could allow feedlots to precision feed to optimize carcass traits. A single nucleotide polymorphism (SNP) in alcohol dehydrogenase 1C (ADH1C) has previously shown promise for this approach but has yet to be effective at a commercial level; therefore we hypothesized a variant in another gene or its interaction with ADH1Cc.-64T>C might be the solution. Genes previously shown to be affected by retinoic acid, a metabolite of vitamin A: aminopeptidase (ANPEP), clusterin (CLU), adipose differentiation-related protein (ADFP), glutathione peroxidase (GPX3), secreted protein, acidic, cysteine-rich (SPARC), and insulin growth factor binding protein 6 (IGFBP6) were sequenced and screened for variants. The ANPEPc.410G>A SNP was selected for genotyping in a population of mixed breed steers (n=988). This population was fed vitamin A at 100% (100VA) or 50% (50VA the NRC recommended level (2200 IU/kg dry matter). No interaction was found with ADH1Cc.-64T>C however, ANPEPc.410G>A affected carcass yield (P<0.01; AA=2.47±0.03, GA=2.36±0.03, GG=2.14±0.08), marbling score (P<0.01; AA=397.2±2.7, GA=388.6±3.3, GG=370.4±7.2), and fat (P<0.01; AA=8.52±0.17 GA=7.58±0.21, GG=7.04±0.44; mm). Vitamin A also had an effect on backfat (P<0.05; 100VA= 8.13±0.24, 50VA = 7.35±0.25), and an interaction with ANPEPc.410c.G>A affected rib-eye area (P<0.05). The ANPEP SNP was genotyped in a second population of mixed breed steers (N=708) fed a standard feedlot ration with the NRC recommended level of vitamin A. There was an association with yield, marbling, fat, and rib-eye area (P<0.01). The AA genotype was more marbled, while GG animals were leaner with higher yields. Interestingly, ANPEPc.410G>A is the fourth variant in a haplotype containing twelve SNPs that are in linkage disequilibrium in exon 1 and intron 1. This was confirmed by sequencing cattle of various breeds from different populations. The three haplotypes could affect gene expression by altering transcription or translation efficiency. Investigation of the functional effects of these variants needs to be completed in order to understand how it alters traits related to feedlot cattle performance. Advisors/Committee Members: Buchanan, Fiona C, Asai-Coakwell, Mika, McKinnon, John J, Bett, Kirstin E, Brook, Ryan K.

Subjects/Keywords: beef; cattle; genetics; variants; marbling; feedlot; carcass; nutrigenomics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hamilton, K. E. 1. (2019). A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/11739

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Hamilton, Kelsey Evelyn 1994-. “A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle.” 2019. Thesis, University of Saskatchewan. Accessed March 04, 2021. http://hdl.handle.net/10388/11739.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Hamilton, Kelsey Evelyn 1994-. “A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle.” 2019. Web. 04 Mar 2021.

Vancouver:

Hamilton KE1. A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle. [Internet] [Thesis]. University of Saskatchewan; 2019. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/10388/11739.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hamilton KE1. A Nutrigenomic Perspective to Search for Gene Variants That Influence Carcass Traits of Feedlot Cattle. [Thesis]. University of Saskatchewan; 2019. Available from: http://hdl.handle.net/10388/11739

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.