+publisher:"University of Saskatchewan" +contributor:("Adams, Gregg P.")

University of Saskatchewan

1. al-shanoon, hayder. Effect of Testosterone on Ovarian Function in Beef Heifers.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-01-330

► Synchronization of follicular wave emergence forms the basis of many reproductive management techniques used in the cattle industry such as estrus synchronization, fixed-time artificial insemination… (more)

▼ Synchronization of follicular wave emergence forms the basis of many reproductive management techniques used in the cattle industry such as estrus synchronization, fixed-time artificial insemination and embryo transfer. Estrogen in combination with progesterone has been used widely for this purpose due to ease of use and efficient, consistent and quick results, irrespective of the status of the dominant follicle or corpus luteum when the treatment is applied. The recent ban by the European Union on use of estrogens in food-producing animals prompted us to examine if testosterone could be used as an alternative drug for follicular wave synchronization. We tested the hypothesis that the administration of testosterone will shorten the life-span of the extant dominant follicle resulting in early emergence of a new follicular wave. We tested two forms of testosterone (conjugated form: testosterone enanthate and unconjugated form: non-esterified free testosterone) and determined that intramuscular injection of oil-based preparation of the unconjugated form caused a sharp rise in plasma concentrations of testosterone followed by a less rapid decline. The pharmacokinetics of testosterone in blood plasma of 16 heifers was determined after two intramuscular injections of 200 mg of unconjugated testosterone (in 4 ml canola oil) at 12 hour intervals. Testosterone C max was 13.9 ng/mL and the distribution half-life of testosterone in the bloodstream was 3.2 days. Plasma testosterone concentrations were elevated within 2 hours, maintained for initial 36 hours and declined to baseline over 13 days. To study the ovarian and endocrine effects of testosterone, heifers (n=6 per group) were given two intramuscular injections of 200 mg of unconjugated testosterone in 4 mL canola oil at 12 hour intervals on Days 1 (T1), 3 (T3) or 6 (T6) of the first follicular wave (Day 0 = ovulation); the Control group was given 4 mL canola oil. Ovarian structures were monitored daily by transrectal ultrasonography over one interovulatory interval and plasma samples were collected. Following treatment, the dominant follicle grew more slowly for the next 5 days in T1 (P=0.05) and T3 (P=0.06) groups compared to the Control group, while the dominant follicle in the T6 group regressed more slowly (P=0.02) than in the Control group. The diameter profile of the dominant follicle of the post-treatment wave (Wave 2) and the ovulatory wave did not differ between treatment and Control groups. Overall, testosterone treatment (T1, T3 and T6 combined) extended the duration of current (Wave 1; P=<0.001) wave in 8 out of 17 heifers compared with the Control group and emergence of the post-treatment wave was not synchronized. Although the interovulatory interval was not affected by the treatments, the proportion of 2-wave cycles tended to be higher (P=0.08) after treatment (T1, T3 and T6 combined) compared to the Control group. The plasma LH concentrations in T1 and T3 groups decreased after treatment, while it did not change in T6 group compared to Control group.… Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Mapletoft, Reuben, Muir, Gillian.

Subjects/Keywords: Testosterone; follicle development; cattle; corpus luteum; ultrasonography; follicular and luteal dynamics; FSH; LH; Pharmacokinetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

al-shanoon, h. (2012). Effect of Testosterone on Ovarian Function in Beef Heifers. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-01-330

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

al-shanoon, hayder. “Effect of Testosterone on Ovarian Function in Beef Heifers.” 2012. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2012-01-330.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

al-shanoon, hayder. “Effect of Testosterone on Ovarian Function in Beef Heifers.” 2012. Web. 03 Mar 2021.

Vancouver:

al-shanoon h. Effect of Testosterone on Ovarian Function in Beef Heifers. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2012-01-330.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

al-shanoon h. Effect of Testosterone on Ovarian Function in Beef Heifers. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-01-330

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

2. Carrasco, Rodrigo. Ovulation-inducing factor/nerve growth factor (OIF/NGF) : Immunohistochemical studies of the bovine ovary and the llama hypothalamus.

Degree: 2016, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2016-01-2420

► The overall objective was to elucidate the mechanism of action of ovulation-inducing factor/nerve growth factor (OIF/NGF) in the reproductive function of spontaneous and induced ovulators,… (more)

▼ The overall objective was to elucidate the mechanism of action of ovulation-inducing factor/nerve growth factor (OIF/NGF) in the reproductive function of spontaneous and induced ovulators, using cow and llama as models. In Study 1, the dynamics of trkA, the high affinity receptor for OIF/NGF, were studied during periovulatory period in cows. Unilateral ovariectomies were performed by colpotomy on Days 2, 4 and 6 of the estrous cycle (Day 0= ovulation), and before and after LH administration. Ovarian samples were processed for immunofluorescent detection of trkA. The intensity and area of immuno-positive staining, and the proportion of immuno-positive cells in both the granulosa and theca layers were higher in dominant than in subordinate follicles (P<0.05). Dominant follicles displayed a different intracellular distribution of trkA from subordinate follicles. The number of positive cells was higher in the developing CL (Day 2 and 4) than in the mature or regressing CL (Day 6, Pre-LH, and Post-LH). In Study 2, the distribution of GnRH neurons in the hypothalamus was examined in female llamas (n = 4). Hypothalamic samples were processed for immunohistochemistry for GnRH. The distribution of GnRH neurons had no evident accumulation in specific hypothalamic nuclei. The majority of GnRH neurons were detected in the anterior and medio-basal hypothalamus (P<0.05). The GnRH neuron fibers were detected primarily in the median eminence and in the medio-basal hypothalamus. In Study 3, the relationship between trkA and GnRH neurons in the llama diencephalon was examined in llama brains (n = 4) obtained in Study 2. Samples were stained using double immunofluorescence. TrkA immuno-reactivity was present in most hypothalamic areas examined; the highest density was found in the diagonal band of Broca and the periventricular nuclei. A low percentage of GnRH cells (1%) showed immuno-reactivity to trkA. Close association between immuno-reactive cells (i.e., GnRH and trkA in the same microscopic field) was detected rarely (3/160 GnRH neurons). We concluded that: 1) the high affinity receptor for OIF/NGF is expressed in greater quantities in dominant than subordinate follicles and in the developing CL; 2) GnRH neurons of llamas are concentrated in the anterior and middle hypothalamus, in close relationship to the third ventricle; and, 3) expression of trkA receptors on GnRH neurons was rare, suggesting that the ovulatory effect of OIF/NGF is not via direct interaction with GnRH neurons. Advisors/Committee Members: Adams, Gregg P., Singh, Jaswant, Roger, Pierson A..

Subjects/Keywords: Ovulation-inducing factor (OIF/NGF); Nerve growth factor (NGF); Ovary; Bovine; Llama; Hypothalamus.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carrasco, R. (2016). Ovulation-inducing factor/nerve growth factor (OIF/NGF) : Immunohistochemical studies of the bovine ovary and the llama hypothalamus. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2016-01-2420

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carrasco, Rodrigo. “Ovulation-inducing factor/nerve growth factor (OIF/NGF) : Immunohistochemical studies of the bovine ovary and the llama hypothalamus.” 2016. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2016-01-2420.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carrasco, Rodrigo. “Ovulation-inducing factor/nerve growth factor (OIF/NGF) : Immunohistochemical studies of the bovine ovary and the llama hypothalamus.” 2016. Web. 03 Mar 2021.

Vancouver:

Carrasco R. Ovulation-inducing factor/nerve growth factor (OIF/NGF) : Immunohistochemical studies of the bovine ovary and the llama hypothalamus. [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2016-01-2420.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carrasco R. Ovulation-inducing factor/nerve growth factor (OIF/NGF) : Immunohistochemical studies of the bovine ovary and the llama hypothalamus. [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/ETD-2016-01-2420

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

3. Cervantes, Miriam P. The rabbit as an animal model for the study of ovulation-inducing factor.

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2011-08-63

► Ovulation–inducing factor (OIF) is a seminal plasma protein that has been found in the ejaculate of different species. Evidence suggests that OIF and its function… (more)

▼ Ovulation–inducing factor (OIF) is a seminal plasma protein that has been found in the ejaculate of different species. Evidence suggests that OIF and its function may be conserved among species. Our general objective was to develop a rabbit model for the study of OIF in seminal plasma. In the first study, we conducted two experiments to validate the use of ultrasound biomicroscopy as a method for assessing ovarian structures in rabbits, and to develop a method that permits serial noninvasive repetitive ultrasound evaluation of ovarian structures in vivo in rabbits. In Experiment 1, the number and size of follicles ≥ 0.6 mm and corpora lutea (CL) detected by ultrasound biomicroscopy (UBM) ex situ were correlated with those detected by histology in each pair of ovaries from 4 female New Zealand White rabbits (5-5.5 months old) given an ovulation-inducing treatment. In Experiment 2, we translocated the ovaries of female New Zealand white rabbits (n=12; 5 months old) to a subcutaneous position in order to develop a method that permits serial UBM evaluation of ovarian structures in vivo in rabbits. Results showed strong correlations (P < 0.05) between UBM and histology in all the ovarian variables evaluated in Experiment 1, and also showed that ovarian structures are easily identified using UBM in vivo in the rabbits submitted to the surgical approach performed in Experiment 2. In the second study, we conducted two experiments to test the hypothesis that llama and rabbit seminal plasma elicits a surge of LH release and is responsible for inducing ovulation in rabbits. In Experiment 1, we compared the effect of an intramuscular administration of saline, GnRH, llama or rabbit seminal plasma in female New Zealand White rabbits (n=4-6 per group, 5.5 months old) that were group-housed. In Experiment 2, we compared the effects of the same treatments in rabbits (n=5-7 per group, 5.5 months old) that were individually caged. Ovulation and CL formation occurred in most rabbits regardless of the treatment given when animals were group housed, while rabbits given similar treatment but individually housed did not ovulate. In the GnRH group, a surge in plasma LH concentrations was observed in all the rabbits, followed by CL formation and an increase in plasma progesterone concentrations. In summary, we developed a rabbit model for the study of OIF, where ovarian structures can be evaluated in vivo by UBM. Results did not support the hypothesis that OIF in seminal plasma elicits ovulation in rabbits. Further studies are needed to determine the effects of dose and route of administration of seminal plasma in rabbits. Advisors/Committee Members: Adams, Gregg P., Muir, Gillian, Singh, Jaswant, Lessard, Carl, Baerwald, Angie.

Subjects/Keywords: Rabbit; seminal plasma; ovulation-inducing factor; ultrasound biomicroscopy; ex situ; in vivo.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cervantes, M. P. (2011). The rabbit as an animal model for the study of ovulation-inducing factor. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2011-08-63

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cervantes, Miriam P. “The rabbit as an animal model for the study of ovulation-inducing factor.” 2011. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2011-08-63.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cervantes, Miriam P. “The rabbit as an animal model for the study of ovulation-inducing factor.” 2011. Web. 03 Mar 2021.

Vancouver:

Cervantes MP. The rabbit as an animal model for the study of ovulation-inducing factor. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2011-08-63.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cervantes MP. The rabbit as an animal model for the study of ovulation-inducing factor. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/ETD-2011-08-63

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

4. Cervantes Flores, Miriam Pilar. IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae).

Degree: 2016, University of Saskatchewan

URL: http://hdl.handle.net/10388/7656

► The studies presented in this thesis were designed to develop efficient protocols for collecting competent oocytes and to produce disease-free wood bison embryos by in… (more)

▼ The studies presented in this thesis were designed to develop efficient protocols for collecting competent oocytes and to produce disease-free wood bison embryos by in vitro fertilization and culture. In Chapter 3, the in vivo and in vitro maturational characteristics of cumulus-oocyte complexes (COC) collected from live wood bison during the anovulatory and ovulatory seasons were compared. The stages of nuclear maturation: germinal vesicle (GV), germinal vesicle break down (GVBD), metaphase I (MI) and metaphase II (MII) were determined in wood bison oocytes using anti-Lamin AC/DAPI staining. Additionally, the optimal interval of time after human chorionic gonadotrophin (hCG) treatment required for in vivo oocyte maturation in wood bison was determined. Nuclear maturation occurred more rapidly during in vitro versus in vivo maturation, but was associated with less cumulus cell expansion than with in vivo maturation. In vitro oocyte maturation was maximal after 24 h of in vitro maturation. In vivo oocyte maturation was more complete at 30 than 24 h after hCG treatment. Season had no effect on the maturational capacity of wood bison oocytes. Competence of the in vitro (Chapter 4) or in vivo (Chapter 5) matured oocytes to develop to the blastocyst stage in culture was evaluated in subsequent studies. In Chapter 4, the hypothesis that the morphological characteristics of wood bison cumulus-oocyte complexes (COC) affect the ability of the immature oocyte to develop in vitro following in vitro fertilization was tested. The effect of extending from 48 h to 72 h the FSH starvation period after superstimulation (FSH diluted in 0.5% hyaluronan) on number and size of the follicles at the time of collection by transvaginal ultrasound-guided follicular aspiration, on COC morphological characteristic, and on blastocyst development rate, was also investigated. Compact COC classified as good (>3 layers of cumulus cells) resulted in the highest blastocyst rate following in vitro maturation, fertilization and culture. There was no effect of extending the FSH starvation period by 24 h on the number of follicles ≥ 5 mm at the time of collection, the morphology of the COC or blastocyst rate on Day 7 or 8 after fertilization. The morphological characteristics of immature wood bison COC affect oocyte in vitro developmental potential to the blastocyst stage. In Chapter 5, the effect of an additional 4 h of in vitro maturation of in vivo matured oocytes collected 30 h after hCG treatment on subsequent embryo development was evaluated. In addition, the effects of extending the interval between hCG treatment and COC collection from 30 to 34 h on in vitro embryo production was evaluated. Results confirmed that an additional short period of in vitro maturation, or an extended period of in vivo maturation increased in vitro embryo production rates in wood bison. In the final chapter (Chapter 6), the effectiveness of the IETS washing procedures with or without antibiotics for removing Brucella abortus from in vitro-produced embryos infected in… Advisors/Committee Members: Machin, Karen, Adams, Gregg P, Anzar, Muhammad, Mapletoft, Reuben J, Mastromonaco, Gabriela F.

Subjects/Keywords: Blastocyst rate; In vitro embryo production; Wood bison

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cervantes Flores, M. P. (2016). IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae). (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7656

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cervantes Flores, Miriam Pilar. “IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae).” 2016. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/7656.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cervantes Flores, Miriam Pilar. “IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae).” 2016. Web. 03 Mar 2021.

Vancouver:

Cervantes Flores MP. IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae). [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/7656.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cervantes Flores MP. IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae). [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/7656

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

5. Bogle, Orleigh Addelecia. Nerve growth factor: its role in male fertility as an ovulation inducer.

Degree: 2016, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2016-12-2359

► The studies presented in this thesis were designed to elucidate whether the abundance of ovulation-inducing factor/nerve growth factor (OIF/NGF) in alpaca semen can be used… (more)

▼ The studies presented in this thesis were designed to elucidate whether the abundance of ovulation-inducing factor/nerve growth factor (OIF/NGF) in alpaca semen can be used as a biomarker to predict male fertility. The neurotrophin, OIF/NGF has been identified in camelid, cattle and human semen. It is only in camelids, however, that an ovulation-inducing role for OIF/NGF has been described. The information gathered from several studies clearly demonstrate that this protein is the stimulus responsible for initiating the ovulatory cascade in camelids. In addition, intramuscular administration of OIF/NGF resulted in a dose-dependent response in terms of ovulation rate, corpus luteum (CL) lifespan, luteinizing hormone (LH) and progesterone secretion. I hypothesized that the quantity of OIF/NGF differs among male alpacas and this abundance arbitrates ovulation and pregnancy rates as well as CL formation and function. To substantiate this hypothesis, the following questions were answered: 1) can OIF/NGF in alpaca semen be quantified using a radioimmunoassay; 2) does the concentration and total abundance of OIF/NGF in alpaca semen vary within and among male ejaculates; 3) what is the glandular source of OIF/NGF that contributes to the male ejaculate; 4) is OIF/NGF concentration or abundance related to parameters associated with male fertility; 5) can OIF/NGF concentration or total abundance in the ejaculate discriminate fertile and subfertile males using both retrospective and prospective approaches; and 6) can power Doppler ultrasonography be used to assess the luteotrophic effect of OIF/NGF in tissue vasculature of the developing CL? I discovered that the source and the amount of OIF/NGF varies among species. In llamas, OIF/NGF is produced by both the corpus and disseminate portions of the prostate gland. In rats, OIF/NGF was detected in testis interstitial cells and in the lumen of the coagulating gland (anterior prostate). Ovulation-inducing factor/NGF secretion by the ampullae and vesicular glands contributed to its presence in bull (cattle and bison) ejaculates. In elk and white tail deer, OIF/NGF was detected in the ampullae and prostate glands, respectively. To gain an understanding of the abundance of OIF/NGF in ejaculates and changes in its concentration within and among males, OIF/NGF levels in semen were quantified using the radioimmunoassay. The assay developed exhibited parallel displacement curves among recombinant NGF, OIF/NGF purified from llama seminal plasma, llama and bull (cattle) seminal plasma. Ovulation-inducing factor/NGF comprised a greater percentage of the total protein found in camelid ejaculates than in cattle. Ovulation-inducing factor/NGF concentration correlated positively with sperm concentration and negatively with pH and semen volume, while total abundance of OIF/NGF was related to total prostate area and OIF/NGF concentration. Although a correlation was found between sperm concentration, neither OIF/NGF concentration nor total abundance was associated with higher ovulation, pregnancy… Advisors/Committee Members: Adams, Gregg P., Singh, Jaswant, Pierson, Roger A., Verge, Valerie M., Chizen, Donna.

Subjects/Keywords: Ovulation inducing factor (OIF); Nerve growth factor (NGF); camelids; semen; seminal plasma; protein; ovulation; pregnancy; fertility; sperm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bogle, O. A. (2016). Nerve growth factor: its role in male fertility as an ovulation inducer. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2016-12-2359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bogle, Orleigh Addelecia. “Nerve growth factor: its role in male fertility as an ovulation inducer.” 2016. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2016-12-2359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bogle, Orleigh Addelecia. “Nerve growth factor: its role in male fertility as an ovulation inducer.” 2016. Web. 03 Mar 2021.

Vancouver:

Bogle OA. Nerve growth factor: its role in male fertility as an ovulation inducer. [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2016-12-2359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bogle OA. Nerve growth factor: its role in male fertility as an ovulation inducer. [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/ETD-2016-12-2359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

6. Palomino Cano, Jesus Manuel. SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON (Bison bison athabascae): TOOLS TO PRODUCE DISEASE-FREE EMBRYOS.

Degree: 2015, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2015-12-2351

► Reclamation of Canada’s threatened wood bison (Bison bison athabascae) herd is complicated by cattle diseases. As part of an overall goal to conserve bison genetics,… (more)

▼ Reclamation of Canada’s threatened wood bison (Bison bison athabascae) herd is complicated by cattle diseases. As part of an overall goal to conserve bison genetics, five studies were conducted to develop or adapting present reproductive technologies to produce disease-free in vivo-derived wood bison embryos. In Chapter 4, the efficacy of pLH and hCG for inducing ovulation and whether the effect was related to the size of the dominant follicle at the time of treatment was examined in wood bison during the anovulatory season . Ovulation rate with hCG (94%) was nearly two times greater than with pLH (54%), and bison with a growing follicle of ≥10 mm had a greater ovulatory response than those of 8-9 mm. In Chapter 5, the efficacy of pLH and hCG after superstimulation with single or two doses (48 hours apart) of FSH diluted in 0.5% hyaluronan was determined in wood bison. A greater superovulatory ovarian response was found in cows treated with hCG vs. LH during the anovulatory and ovulatory seasons (6.6 vs. 2.8 and 6.3 vs. 3.8 corpora lutea respectively). In addition, dividing the dose of FSH two resulted in greater superovulatory response in wood bison. However, the number of corpora lutea was still lower than expected as compared to cattle using the same two dose method of superovulation (15 corpora lutea; Tribulo et al., 2012). Therefore, in Chapter 6, the effect of the addition of a low dose of eCG at the end of the superstimulation protocol on ovarian response and embryo quality was examined. Although the number of ova/embryos recovered was higher in this study when compared with previous reports in wood bison, no effect of eCG on the number of corpora lutea and embryo quality was found. In Chapters 5 and 6, the effect of exogenous progesterone on embryo quality in wood bison during the anovulatory season was evaluated. We found that progesterone did not improve the number of freezable embryos in either study. In Chapter 7, the effect of lengthening of FSH treatment protocol on superovulatory response and embryo quality during the ovulatory and anovulatory seasons was examined. There was no effect of lengthening the FSH treatment protocol on ovarian response and embryo quality during the anovulatory season. However, embryo quality and ovulation rate were increased by the lengthened treatment protocol during the ovulatory season. Additionally, more freezable embryos (Grades 1 and 2) were obtained during the ovulatory season (1.8 embryos) vs. the anovulatory season (0.3 embryos). Overall, results confirm that superovulation can be performed in wood bison throughout the year, but a higher number of freezable embryos were obtained during the ovulatory season. The final chapter (Chapter 8) focused in the production of disease-free embryos in wood bison. Following superovulation, in vivo-derived wood bison embryos were exposed in vitro to Brucella abortus biovar 1. After incubation, embryos were submitted to the 10-step washing procedures recommended by the IETS to remove the pathogen. When the washing medium contained… Advisors/Committee Members: Adams, Gregg P., Mapletoft, Reuben J., Woodbury, Murray R., McCorkell, Robert B., Seidel, George E..

Subjects/Keywords: Wood bison; superovulation; embryo collection; embryo washing; seasonality; ovulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Palomino Cano, J. M. (2015). SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON (Bison bison athabascae): TOOLS TO PRODUCE DISEASE-FREE EMBRYOS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2015-12-2351

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Palomino Cano, Jesus Manuel. “SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON (Bison bison athabascae): TOOLS TO PRODUCE DISEASE-FREE EMBRYOS.” 2015. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2015-12-2351.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Palomino Cano, Jesus Manuel. “SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON (Bison bison athabascae): TOOLS TO PRODUCE DISEASE-FREE EMBRYOS.” 2015. Web. 03 Mar 2021.

Vancouver:

Palomino Cano JM. SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON (Bison bison athabascae): TOOLS TO PRODUCE DISEASE-FREE EMBRYOS. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2015-12-2351.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Palomino Cano JM. SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON (Bison bison athabascae): TOOLS TO PRODUCE DISEASE-FREE EMBRYOS. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/ETD-2015-12-2351

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

7. Tavares Krause, Ana Rita 1985-. Ovarian Response, Follicular Function and Oocyte Developmental Competence in Gonadotropin Treated Prepubertal Calves.

Degree: 2019, University of Saskatchewan

URL: http://hdl.handle.net/10388/12490

► Sexual immaturity during the prepubertal period in cattle is characterized by low pulse frequency of LH, anovulatory waves of follicle development, absence of corpora lutea… (more)

▼ Sexual immaturity during the prepubertal period in cattle is characterized by low pulse frequency of LH, anovulatory waves of follicle development, absence of corpora lutea (i.e., progesterone) and oocytes of reduced developmental capacity in vitro when compared to oocytes of sexually mature animals. However, calf ovaries are responsive to exogenous gonadotropin treatment early in life, and the use of prepubertal animals as a source of oocytes for in vitro embryo production may have significant potential to decrease generation interval and accelerate the rate of genetic gain. The overall objective of this thesis was to investigate the effect of exogenous FSH treatment on the ovarian response, hormonal profiles, ovulation and oocyte developmental competence in prepubertal calves. In the first study (Chapter 3) the effect of cumulative dose (200 mg vs. 350 mg) and duration (4 vs. 7 days) of FSH treatment on the ovarian response and the number of spontaneous and induced ovulations in 5-month-old calves were compared. Calves (n=24) were selected for gonadotropin treatment from a group of spring-born calves (n=51) based on the antral follicle counts (AFC) at the time of wave emergence. Calves were classified in low, medium and high AFC, and the ones in the medium classification (250.8, range of 20 to 32 follicles) were used in this study. At the end of the FSH treatment and 24 hours after treatment with pLH, the number of follicles  9 mm was greater in the 7-day than in the 4-day treatment group and in calves given a cumulative dose of 350 mg of FSH compared to those given 200 mg. Spontaneous ovulations were observed in 14 calves between Day 4 of FSH treatment and 12-hours post-LH treatment. The number of total and spontaneous ovulations was higher in the 7-day treatment groups than in the 4-day groups, and the number of spontaneous ovulations was higher in calves given a cumulative dose of 200 mg FSH than 350 mg. Numbers of ovulations in response to exogenous LH did not differ among groups. In the second study (Chapter 4), data showed in prepubertal calves (n=46) that the number of follicles at the beginning of a wave was predictive of the number recruited into subsequent waves and that after FSH treatment, the number of medium and large sized follicles available for follicular aspiration was positively associated with the number of follicles  1 mm at the time of wave emergence. In calves with low (n=12) and high (n=10) AFC at wave emergence, 7 days of FSH treatment resulted in a higher number of large than small size follicles than the 4 days of FSH treatment. High AFC at wave emergence resulted in a greater number of follicles  6 mm available for aspiration and a greater number of cumulus oocyte complexes (COC) collected than low AFC. The third study (Chapter 5) was designed to investigate the relationship between the antral follicular counts and plasma concentrations of AMH and FSH at the time of wave emergence in prepubertal calves and to compare the effects of age and duration of gonadotropin treatment on… Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P, Mapletoft, Reuben J, Dias, Fernanda CF, Misra, Vikram, Unniappan, Suraj.

Subjects/Keywords: follicular growth; superstimulation; puberty; oocyte competence; cattle; gonadotropin

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APA (6^th Edition):

Tavares Krause, A. R. 1. (2019). Ovarian Response, Follicular Function and Oocyte Developmental Competence in Gonadotropin Treated Prepubertal Calves. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12490

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tavares Krause, Ana Rita 1985-. “Ovarian Response, Follicular Function and Oocyte Developmental Competence in Gonadotropin Treated Prepubertal Calves.” 2019. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/12490.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tavares Krause, Ana Rita 1985-. “Ovarian Response, Follicular Function and Oocyte Developmental Competence in Gonadotropin Treated Prepubertal Calves.” 2019. Web. 03 Mar 2021.

Vancouver:

Tavares Krause AR1. Ovarian Response, Follicular Function and Oocyte Developmental Competence in Gonadotropin Treated Prepubertal Calves. [Internet] [Thesis]. University of Saskatchewan; 2019. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/12490.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tavares Krause AR1. Ovarian Response, Follicular Function and Oocyte Developmental Competence in Gonadotropin Treated Prepubertal Calves. [Thesis]. University of Saskatchewan; 2019. Available from: http://hdl.handle.net/10388/12490

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Dias, Fernanda. Effects of follicular aging and duration of superstimulation on oocyte competence and granulosa cell gene expression in cattle.

Degree: 2013, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2013-06-1110

► A prolonged growth phase of the ovulatory follicle results in follicular aging. Whether follicular aging is detrimental or beneficial to oocyte competence is not fully… (more)

▼ A prolonged growth phase of the ovulatory follicle results in follicular aging. Whether follicular aging is detrimental or beneficial to oocyte competence is not fully known. The objective of this thesis is to investigate the effects of follicular aging on oocyte competence and granulosa cell gene expression in cattle. Four sets of experiments were designed to address the objective. The following hypotheses were tested during the course of these studies: 1) oocyte competence will improve by the longer growing phase but will be adversely affected by FSH starvation, 2) follicles that undergo superstimulation will have different gene expression than dominant follicles from a natural cycle, 3) extending the superstimulation protocol by 3 days will allow follicles to mature better and 4) markers of maturity, cellular health and survival will be turned off by FSH starvation. The objective of the first study (Chapter 3) was to determine the effects of extending the length of superstimulation and follicular aging on oocyte competence by in vitro embryo production. Multiple follicles were allowed to grow for 4 (Short FSH) or 7 days (Long FSH) under the treatment of 8 or 14 injections of FSH (at 12-hour intervals), respectively. Multiple follicles in the FSH starvation group were allowed to grow for 7 days but FSH was provided for only the first 4 days of superstimulation. Extending the duration of follicular growth by superstimulation resulted in a greater number of ≥9 mm follicles and in 2.5 more transferable embryos per animal (morulae+blastocysts) at Day 9 of in vitro embryo culture. The FSH starvation resulted in a greater proportion of poor quality oocytes lower cleavage rate and lower embryonic development. Microarray analysis was used to assess the effect of superstimulation (Chapter 4), follicular aging (Chapter 5) and FSH starvation (Chapter 6) on the gene expression profile of superstimulated granulosa cells. Gene expression of granulosa cells from the post-LH preovulatory dominant follicle was compared (Chapter 4) with those from follicles of the same status after a standard 4-day superstimulation (same protocol as Short FSH group from Chapter 3). A total of 190 genes were down-regulated and 280 genes were upregulated in the superstimulated group when compared with the reference (non-superstimulated control). Data analysis showed that superstimulated follicles are still in a growing phase compared to untreated dominant follicles (most of the upregulated genes are related to matrix remodeling due to tissue proliferation) and did not respond to LH properly (down regulation of LH gene markers). Four-day superstimulation also disturbed genes related to angiogenesis and activated oxidative stress response genes. Extending the superstimulation protocol (7 days; same protocol as Long FSH from Chapter 3) allowed more time for follicles to leave the growing stage and properly respond to LH surge (most of the upregulated genes in the Long FSH group are markers of post LH surge) when compared to the standard 4 day… Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Misra, Vikram, Sirard, Marc-Andre.

Subjects/Keywords: cattle; FSH; follicle dynamics; follicular aging; gene expression; granulosa cells; oocyte competence; superstimulation

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APA (6^th Edition):

Dias, F. (2013). Effects of follicular aging and duration of superstimulation on oocyte competence and granulosa cell gene expression in cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-06-1110

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dias, Fernanda. “Effects of follicular aging and duration of superstimulation on oocyte competence and granulosa cell gene expression in cattle.” 2013. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2013-06-1110.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dias, Fernanda. “Effects of follicular aging and duration of superstimulation on oocyte competence and granulosa cell gene expression in cattle.” 2013. Web. 03 Mar 2021.

Vancouver:

Dias F. Effects of follicular aging and duration of superstimulation on oocyte competence and granulosa cell gene expression in cattle. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2013-06-1110.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dias F. Effects of follicular aging and duration of superstimulation on oocyte competence and granulosa cell gene expression in cattle. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-06-1110

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Caunce, Serena L. THREE-DIMENSIONAL ANALYSIS OF COLOUR DOPPLER ULTRASOUND IMAGES: THE RELATIONSHIP OF VASCULAR FLOW TO OVARIAN STRUCTURES AND THEIR FUNCTION IN BUFFALO COWS AND BEEF CALVES.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/7992

► The main objective of this thesis was to develop a three-dimensional method to assess ovarian structures and relate their vascularity to their function (i.e. hormone… (more)

▼ The main objective of this thesis was to develop a three-dimensional method to assess ovarian structures and relate their vascularity to their function (i.e. hormone production, oocyte quality, ovulatory capacity, etc.). This novel method uses video segments recorded after free-hand movement of the routine linear-array transducer, obtained vascularity index values are independent of speed of transducer movement, and the method does not require a-priori operator selection of images, i.e. is an objective method. In Study 1, colour Doppler ultrasonography was used to assess the ovaries of water buffalo (Bubalus bubalis) who were induced to ovulate using a gonadotropin releasing hormone analogue. Ultrasonographic examinations were performed daily to assess ovulation and subsequent corpus luteum (CL) development. Recorded cineloops were exported from the ultrasound machine and analyzed on a personal computer equipped with imaging software from two sources, Fiji (ImageJ) and Imaris. Fiji was used to perform conventional two-dimensional image analysis and Imaris was used to develop a new three-dimensional analysis method that does not require operator selection of images (i.e. objective method). Verification of the three-dimensional method was performed using two-dimensional data. Buffalo that did not ovulate within 24 hours of artificial insemination (40h from time of gonadotrophin releasing hormone analogue treatment) (n=3) tended to have lower change in vascular flow (P=0.06) to their preovulatory follicles when compared to those that ovulated (n=13) using the three-dimensional analysis method. In Study 2, power Doppler ultrasonography was used to assess the ovaries of 4-month-old prepubertal beef heifers (Bos taurus) who were superstimulated using two follicle stimulating hormone (FSH) protocols. Ultrasonographic scans were performed on the day of luteinizing hormone (LH) treatment, the day of oocyte collection, and the third and seventh day following follicular aspiration. The three-dimensional method developed in Study 1 was used to calculate vascularity indices for the four time points. Ovarian vascularity was then related to the expansion of the cumulus-oocyte-complexes that were retrieved, as well as the subsequent luteal tissue that developed following follicle aspiration. Luteal vascularity index measured at three days following follicular aspiration were related to the rise in plasma progesterone concentration over time (from 24 h following LH to day seven after aspiration) (r=0.65, P<0.01). Prepubertal calves demonstrated an increase in ovarian vascularity index under the influence of exogenous LH that was similar to adult animals. Additionally, an increased duration of superstimulation (seven days instead of four) was important for calves in the development of luteal tissue on day seven, luteal vascularity and progesterone production. In conclusion, three-dimensional image analysis of colour Doppler ultrasound images was superior to two-dimensional in detecting follicles that did not ovulate. This method… Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P, Card, Claire, Honaramooz, Ali.

Subjects/Keywords: colour Doppler ultrasonography; three-dimensional volumetric analysis; ovary; buffalo; reproduction; follicle; blood-flow; vascularity; CL; prepubertal cattle; calf reproduction; ovarian vascularity; FSH stimulation; follicle development; luteal development; cumulus-oocyte-complex; ovum pick-up

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APA (6^th Edition):

Caunce, S. L. (2017). THREE-DIMENSIONAL ANALYSIS OF COLOUR DOPPLER ULTRASOUND IMAGES: THE RELATIONSHIP OF VASCULAR FLOW TO OVARIAN STRUCTURES AND THEIR FUNCTION IN BUFFALO COWS AND BEEF CALVES. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7992

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Caunce, Serena L. “THREE-DIMENSIONAL ANALYSIS OF COLOUR DOPPLER ULTRASOUND IMAGES: THE RELATIONSHIP OF VASCULAR FLOW TO OVARIAN STRUCTURES AND THEIR FUNCTION IN BUFFALO COWS AND BEEF CALVES.” 2017. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/7992.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Caunce, Serena L. “THREE-DIMENSIONAL ANALYSIS OF COLOUR DOPPLER ULTRASOUND IMAGES: THE RELATIONSHIP OF VASCULAR FLOW TO OVARIAN STRUCTURES AND THEIR FUNCTION IN BUFFALO COWS AND BEEF CALVES.” 2017. Web. 03 Mar 2021.

Vancouver:

Caunce SL. THREE-DIMENSIONAL ANALYSIS OF COLOUR DOPPLER ULTRASOUND IMAGES: THE RELATIONSHIP OF VASCULAR FLOW TO OVARIAN STRUCTURES AND THEIR FUNCTION IN BUFFALO COWS AND BEEF CALVES. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/7992.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Caunce SL. THREE-DIMENSIONAL ANALYSIS OF COLOUR DOPPLER ULTRASOUND IMAGES: THE RELATIONSHIP OF VASCULAR FLOW TO OVARIAN STRUCTURES AND THEIR FUNCTION IN BUFFALO COWS AND BEEF CALVES. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7992

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Vassena, Rita. Developmental competence of bovine oocytes: follicular status and ultrasound image characteristics.

Degree: 2001, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-08092012-083123

► This thesis investigates the relationship between the physiological phase of follicles in cattle, their echotexture attributes and the developmental competence of the oocytes that they… (more)

…University of Saskatchewan (SYNERGYNE, Version 2.8, WHIRL, Saskatoon, SK, Canada; ©Pierson…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vassena, R. (2001). Developmental competence of bovine oocytes: follicular status and ultrasound image characteristics. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08092012-083123

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vassena, Rita. “Developmental competence of bovine oocytes: follicular status and ultrasound image characteristics.” 2001. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-08092012-083123.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vassena, Rita. “Developmental competence of bovine oocytes: follicular status and ultrasound image characteristics.” 2001. Web. 03 Mar 2021.

Vancouver:

Vassena R. Developmental competence of bovine oocytes: follicular status and ultrasound image characteristics. [Internet] [Thesis]. University of Saskatchewan; 2001. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-08092012-083123.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vassena R. Developmental competence of bovine oocytes: follicular status and ultrasound image characteristics. [Thesis]. University of Saskatchewan; 2001. Available from: http://hdl.handle.net/10388/etd-08092012-083123

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Khan, Muhammad. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.

Degree: 2014, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2014-01-1364

► Advanced maternal age has been shown to influence follicular and luteal dynamics in bovine ovary resulting in reduced fertility. The overall objective of the four… (more)

▼ Advanced maternal age has been shown to influence follicular and luteal dynamics in bovine ovary resulting in reduced fertility. The overall objective of the four studies presented in this thesis is to identify the maternal age-associated transcriptional changes in granulosa cells of the dominant follicles during follicle development. In the first study, mRNA expression levels of housekeeping genes were measured by real–time quantitative PCR (RT-qPCR) in granulosa cells of dominant follicles and FSH-stimulated follicles to select and validate suitable reference genes for relative gene expression analyses during maternal and follicular aging. Stability of six reference genes (GAPDH, ACTB, EIF2B2, UBE2D2, SF3A1 and RNF20) was analyzed using GeNorm, DeltaCT and NormFinder programs and comprehensive ranking order was determined based on these programs. Geometric mean of multiple genes (UBE2D2, EIF2B2, GAPDH and SF3A1) was more appropriate reference control than individual genes for the comparison of relative gene expression among dominant and FSH-stimulated follicles during maternal and/or follicular aging studies. In the second study, maternal age-associated changes in the transcriptome of granulosa cells recovered at the time of selection of the dominant follicle from aged (n=3) and young cows (n=3) were determined by EmbryoGENE bovine oligo-microarrays (EMBV3, Agilent Technology). The mRNA expression of five transcripts (CYP19A1, PCNA, GJA1, TPM2, and VNN1) was confirmed in a different set of granulosa cell samples by RT-qPCR to validate microarray data. A total of 169 genes/isoforms were differentially expressed (≥ 2-fold-change; P ≤ 0.05) in aged cows vs. young cows. These transcripts revealed inefficient 1) control of gonadotropins, and gonadotropin-induced changes in the cytoskeleton and extracellular matrix, 2) lipid metabolism and steroidogenesis 3) cell proliferation, cell cycle control and intercellular communication, and 4) higher oxidative stress responses in aged cows vs. young cows. In the third study, changes in the transcriptome of granulosa cells of the preovulatory follicle 24 h after LH treatment from aged (n= 3) and young (n=3) were determined. A total of 1340 genes were expressed differentially (≥ 2-fold change; P ≤ 0.05) in aged cows vs. young cows. The mRNA expression of five transcripts (RGS2, PTGS2, TNFAIP6, VNN1, NR5A2 and GADD45B) was confirmed in a different set of granulosa cell samples to validate microarray data. These transcripts were related to delayed 1) response to LH treatment 2) cellular differentiation and luteinization and 3) progesterone synthesis. Intra-follicle levels of progesterone were lower (P < 0.05) in aged cows compared to young and mid-aged cows. The fourth study compared the aged-associated changes in the transcriptome of granulosa cells during follicle development from the time of dominant follicle selection to preovulatory stage (24 h after LH). In comparison to young cows, aged cows expressed fewer differentially expressed genes/isoforms (1206 vs. 2260, respectively)… Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Sirard, Marc A., Misra, Vikram, Anzar, Muhammad.

Subjects/Keywords: Maternal age; Granulosa cells; Dominant follicle at selection; Preovulatory follicle; Reference genes; Microarrays; RT-qPCR; Bovine; Luteinizing hormone; Ovariectomy; Follicular aspiration; Transcriptome analysis; Ingenuity Pathway Analysis; Upstream regulators; Estradiol; Progesterone; Follicular fluid; Ultrasonography; Ovulation

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APA (6^th Edition):

Khan, M. (2014). Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-01-1364

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Muhammad. “Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.” 2014. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2014-01-1364.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Muhammad. “Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.” 2014. Web. 03 Mar 2021.

Vancouver:

Khan M. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2014-01-1364.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan M. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-01-1364

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Yapura, Maria. Development of a non-steroidal aromatase inhibitor-based protocol for the control of ovarian function using a bovine model.

Degree: 2013, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2013-06-1065

► Five studies were designed to characterize the effects of a non-steroidal aromatase inhibitor, letrozole, on ovarian function in cattle. The general hypothesis was that non-steroidal… (more)

▼ Five studies were designed to characterize the effects of a non-steroidal aromatase inhibitor, letrozole, on ovarian function in cattle. The general hypothesis was that non-steroidal aromatase inhibitors have potential as a steroid-free option for the control of ovarian function for the purposes of fixed-time artificial insemination and embryo production. The specific objectives were to determine the effect of route and vehicle, type of aromatase inhibitor, and duration of aromatase inhibitor treatment (short vs prolonged) on ovarian follicles in cattle, and to test the efficacy of an aromatase inhibitor-based protocol to synchronize ovulation in cattle. In the first experiment, heifers were treated with letrozole intravenously (n=10) or intramuscularly (n=10) or allocated in iv and im control groups (n=5/group). During the second experiment, heifers were divided randomly into two groups (n=15/group) and an intravaginal device containing 1 g of letrozole or a blank device (control) was inserted. The third experiment was designed with the goal of formulating and testing an intravaginal device that provides biologically active circulating concentrations of an aromatase inhibitor for a minimum of 4 days. The biological significance of the pharmacokinetic differences between the letrozole intravaginal devices resulting from the third study was evaluated during the fourth study. A final study was designed to determine the effect of stage of the estrous cycle on the proportion of animals that ovulated and the synchrony of ovulation of heifers treated with an aromatase inhibitor-based ovulation-synchronization protocol and to determine subsequent pregnancy outcomes. In all the studies, the effects of aromatase inhibitor on ovarian function were assessed by transrectal ultrasound examination of the ovaries, and blood samples were collected for hormone concentration determination. Results demonstrated that route of administration, or more precisely, the nature of iii the vehicle used for the administration of letrozole (intravenous, intramuscular depot, short release intravaginal or prolonged release intravaginal) has an impact on the effects of letrozole on hormonal profiles and ovarian dynamics. The intramuscular route appeared to provide a prolonged release of letrozole from the injection site which had a marked effect on estradiol production, dominant follicle lifespan, and CL form and function. Letrozole treatment during the ovulatory follicle wave by means of a gel-based intravaginal releasing device during the second study resulted in more rapidly growing dominant follicles and larger ovulatory follicles, delayed ovulation (by 24 h) of a single follicle and formation of a CL that secreted higher levels of progesterone. A wax-based vehicle allowed for a steady and continuous delivery of the active compound over the treatment period. During the third study, the addition of a letrozole-containing gel coating increased the rate of initial absorption and hastened the increase on plasma concentrations of the active… Advisors/Committee Members: Adams, Gregg P., Mapletoft, Reuben J., Singh, Jaswant, Pierson, Roger, Dragan, Rogan.

Subjects/Keywords: Cattle; aromatase inhibitor, letrozole, synchronization; reproduction

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APA (6^th Edition):

Yapura, M. (2013). Development of a non-steroidal aromatase inhibitor-based protocol for the control of ovarian function using a bovine model. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-06-1065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yapura, Maria. “Development of a non-steroidal aromatase inhibitor-based protocol for the control of ovarian function using a bovine model.” 2013. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2013-06-1065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yapura, Maria. “Development of a non-steroidal aromatase inhibitor-based protocol for the control of ovarian function using a bovine model.” 2013. Web. 03 Mar 2021.

Vancouver:

Yapura M. Development of a non-steroidal aromatase inhibitor-based protocol for the control of ovarian function using a bovine model. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2013-06-1065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yapura M. Development of a non-steroidal aromatase inhibitor-based protocol for the control of ovarian function using a bovine model. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-06-1065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Zwiefelhofer, Eric M 1992-. Use of an aromatase inhibitor for ovarian synchronization in cattle.

Degree: 2020, University of Saskatchewan

URL: http://hdl.handle.net/10388/12567

► Letrozole is a third-generation aromatase inhibitor approved for use in post-menopausal women to treat hormone-responsive breast cancer. Letrozole is used off-label in women for the… (more)

▼ Letrozole is a third-generation aromatase inhibitor approved for use in post-menopausal women to treat hormone-responsive breast cancer. Letrozole is used off-label in women for the induction of ovulation and for use in ovarian stimulation. The overall objective of this thesis was to determine the effect of letrozole on ovarian function using the bovine model. In the first study, letrozole was formulated into a silicone intravaginal device consisting of a small or a large surface area compared to a wax-based device or a control intravaginal device given for 8 days in pubertal heifers. The large-surface area device resulted in the greatest plasma letrozole concentration throughout the treatment period. The biological effect on the ovaries was also most profound in the large surface area group where the dominant follicle was largest at the time of device removal and plasma estradiol concentrations decreased the most during device insertion. In the second study, letrozole residues in milk were characterized in lactating dairy cattle. A silicone letrozole-releasing device (LRD) was inserted for 4 days, blood and milk sampling were performed at 12-hour intervals, and a partial validation for letrozole in milk was done using tandem mass spectrometry. Although letrozole was detected in milk, maximum concentration of letrozole was significantly lower in milk than in plasma. There were no differences in the elimination half-life or time to maximum concentration for letrozole in milk or plasma. The objective of the third study was to determine if letrozole would increase the superovulatory response in cattle. Non-lactating cows were given an LRD or a sham device at the time of transvaginal follicular ablation to induce wave emergence. At wave emergence, cows were given 8 doses of follicle-stimulating hormone at 12-hour intervals and were artificially inseminated 48 hours after prostaglandin (PGF). The LRD group exhibited estrus significantly later and estrus was more synchronous than the sham group. However, the LRD group had a lower proportion of quality one embryos and transferable embryos than the sham group. The objective of the fourth study was to characterize the effect of letrozole on follicular function and oocyte competence in cattle in three experiments. In the first experiment, transvaginal ablation was done to induce wave emergence and an LRD or sham device was given for 4 days followed by follicular aspiration to obtain granulosa cells for gene expression. In the second experiment following a 4-day LRD or sham device, frequent blood collections were done for analysis of plasma luteinizing hormone (LH) concentrations. Oocytes were collected that were either immediately denuded and stained or underwent 24 hours of in vitro maturation followed by staining. In the last experiment, following aspiration, oocytes were subjected to in vitro maturation, fertilization, and culture. There was no difference between treatments for gene expression from granulosa cells. Heifers in the LRD group had a significantly higher LH… Advisors/Committee Members: Adams, Gregg P, Chicoine, Al L, Colazo, Marcos G, Mapletoft, Reuben J, Singh, Jaswant, Unniappan, Suraj.

Subjects/Keywords: aromatase inhibitor; estrous cycle; letrozole

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APA (6^th Edition):

Zwiefelhofer, E. M. 1. (2020). Use of an aromatase inhibitor for ovarian synchronization in cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12567

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zwiefelhofer, Eric M 1992-. “Use of an aromatase inhibitor for ovarian synchronization in cattle.” 2020. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/12567.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zwiefelhofer, Eric M 1992-. “Use of an aromatase inhibitor for ovarian synchronization in cattle.” 2020. Web. 03 Mar 2021.

Vancouver:

Zwiefelhofer EM1. Use of an aromatase inhibitor for ovarian synchronization in cattle. [Internet] [Thesis]. University of Saskatchewan; 2020. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/12567.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zwiefelhofer EM1. Use of an aromatase inhibitor for ovarian synchronization in cattle. [Thesis]. University of Saskatchewan; 2020. Available from: http://hdl.handle.net/10388/12567

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Allaway, Heather. Effects of a Non-Steroidal Aromatase Inhibitor on Human Ovarian Function.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-05-439

► Introduction: Ovarian folliculogenesis occurs in a wave-like pattern of growth during the menstrual cycle. A better understanding of the role of estrogen in folliculogenesis may… (more)

▼ Introduction: Ovarian folliculogenesis occurs in a wave-like pattern of growth during the menstrual cycle. A better understanding of the role of estrogen in folliculogenesis may lead to the development of better contraceptive and fertility protocols. Aromatase inhibitors cause a transient decline in natural estrogen production. Multiple small doses of an aromatase inhibitor have been used during fertility therapy to induce dominant follicle growth and ovulation. Objectives: Our objectives were to elucidate the effects of a single 20 mg dose of an aromatase inhibitor (Letrozole/FemaraTM) on folliculogenesis and test the hypothesis that an AI administered during defined times of the follicular phase or immediately after ovulation would result in atresia of the extant dominant follicle and initiate new wave emergence. Methods: Healthy, reproductive age, female volunteers not taking hormonal contraceptives were recruited (n=41). Ultrasound examinations began on day 4 of the menstrual cycle (day 1=first day of menses). Volunteers were randomized into 1 of 4 groups: treatment at a follicle diameter of 1) 12 mm, 2) 18 mm, 3) the first day following ovulation, or 4) control. Serial ultrasonography and phlebotomy were performed until ovulation in the subsequent cycle. Differences were analyzed using t-test, ANOVA, repeated measures ANOVA, or Kruskal-Wallis ANOVA where appropriate. Results: The dominant follicle in all treatment groups ovulated. There were no differences among experimental groups for peak follicle diameter, follicular growth rate, endometrial thickness at ovulation, or inter-ovulatory interval. Maximum FSH concentrations in the 12 mm, 18 mm and post-OV groups were 12.58 ± 1.41 mIU/mL, 18.62 ± 2.27 mIU/mL, 12.38 ± 1.10 mIU/mL, respectively. Maximum LH concentrations in the 12 mm, 18 mm and post-OV groups were 16.20 ±2.06 mIU/mL, 40.43 ± 4.32 mIU/mL, 16.34 ± 2.59 mIU/mL, respectively. The 18 mm group had higher FSH and LH concentrations (P < 0.02) compared to the 12 mm and post-OV groups. Conclusions: Administration of a single 20 mg dose of an aromatase inhibitor at defined times of the menstrual cycle did not induce dominant follicle regression or new wave emergence. Treatment resulted in continued follicle development, a transient decrease in E2 levels and elevated circulating FSH and LH concentrations. The failure of an aromatase inhibitor to interrupt dominant follicle development suggests a compensatory mechanism for the acute drop in E2 that may involve increased LH and FSH levels. Advisors/Committee Members: Pierson, Roger A., Chizen, Donna R., Baerwald, Angela R., Adams, Gregg P..

Subjects/Keywords: Ovarian follicle; Ovulation; Endometrium; Aromatase Inhibitor

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APA (6^th Edition):

Allaway, H. (2012). Effects of a Non-Steroidal Aromatase Inhibitor on Human Ovarian Function. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-05-439

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Allaway, Heather. “Effects of a Non-Steroidal Aromatase Inhibitor on Human Ovarian Function.” 2012. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2012-05-439.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Allaway, Heather. “Effects of a Non-Steroidal Aromatase Inhibitor on Human Ovarian Function.” 2012. Web. 03 Mar 2021.

Vancouver:

Allaway H. Effects of a Non-Steroidal Aromatase Inhibitor on Human Ovarian Function. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2012-05-439.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Allaway H. Effects of a Non-Steroidal Aromatase Inhibitor on Human Ovarian Function. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-05-439

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Dadarwal, Dinesh. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-11-792

► Hormonal environment in which follicle grows has been shown to affect the oocyte competence. Our objective was to identify factors that affect oocyte competence and… (more)

▼ Hormonal environment in which follicle grows has been shown to affect the oocyte competence. Our objective was to identify factors that affect oocyte competence and characterize the structural and functional changes induced by these factors. Fertility was compared in cattle following alterations in levels of progesterone and length of proestrus during dominant follicle growth. We hypothesized that subluteal-phase progesterone will mitigate the effect of a shorter proestrus on pregnancy rates. A shorter duration of proestrus during a fixed-time AI protocol in cattle resulted in a smaller preovulatory follicle, smaller and less functional CL with lower progesterone secretion, and lower fertility (P<0.01). A subluteal-phase progesterone milieu during ovulatory follicle growth induced higher pregnancy rates (P<0.01) and compensated for the effect of a short proestrus on pregnancy rates. Organelle behavior was characterized in oocytes obtained from follicles at different phases of dominant follicle growth. We hypothesized that ooplasmic organelles undergo changes in population and spatial distribution in a phase-specific manner. The growing phase oocytes have least area of mitochondria in contact with lipid droplets (P=0.04) and a peripheral distribution of lipids compared to an even distribution in oocytes from other phases. The regression phase oocytes showed an increase in mitochondrial number (P=0.03) and even distribution of mitochondria compared to peripheral in other phases. Moreover, oocytes from regression phase had higher (P<0.01) lipid content per unit volume of oocyte than other phases. Effect of follicular aging on nuclear maturation rates and, size and distribution of lipid droplets and mitochondria in in vivo matured oocytes were compared. We hypothesized that follicular aging after FSH starvation will result in maturation failure with accumulation of larger lipid droplets and altered distribution of mitochondria as compared to superstimulation with continued FSH support (4-d and 7-d). A 7-d FSH protocol resulted in greater proportion (P<0.01) of mature oocytes compared to other groups. FSH starvation lead to more poor quality oocytes that had ATP contents similar to short FSH group. Further, organization of mitochondria as intense and bigger clusters (P=0.01) alongwith increased size of lipid droplet (P=0.03) within the oocytes from FSH starvation group might indicate atresia. Effect of maternal aging on mitochondrial numbers, distribution and ATP content of in vivo matured bovine oocytes was studied. We hypothesized that in vivo matured oocytes from old cows will have reduced number of mitochondria, altered distribution of mitochondria and decreased the ATP content compared to those from young cows. Maternally aged oocytes had significantly less ATP content (P=0.01) although mitochondrial population and distribution pattern did not differ. Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Lessard, Carl, Tikoo, Suresh K., Muir, Gillian.

Subjects/Keywords: Oocyte maturation; follicular aging; maternal aging; bovine model

…thankful to University of Saskatchewan and Province of Saskatchewan for the financial support and… …GADVASU, Ludhiana for allowing me to pursue my PhD at University of Saskatchewan. v… …Veterinary and Animal Sciences, Ludhiana and University of Saskatchewan, Saskatoon. Without their…

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APA (6^th Edition):

Dadarwal, D. (2012). FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-11-792

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dadarwal, Dinesh. “FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.” 2012. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2012-11-792.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dadarwal, Dinesh. “FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.” 2012. Web. 03 Mar 2021.

Vancouver:

Dadarwal D. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2012-11-792.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dadarwal D. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-11-792

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Tribulo, Paula. THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-10-766

► Three experiments were designed to gain an understanding of the role of ovulation-inducing factor (OIF) present in bovine seminal plasma. Within species, seminal plasma was… (more)

▼ Three experiments were designed to gain an understanding of the role of ovulation-inducing factor (OIF) present in bovine seminal plasma. Within species, seminal plasma was pooled from 1 to 4 ejaculates per male (n=160 bulls, n=4 llamas in Experiments 1 and 2, and n=95 bulls in Experiment 3). The volume of seminal plasma used for treatment was adjusted to a total dose of 250 µg of OIF. Experiment 1 was done to verify the bioactivity of OIF in bovine seminal plasma. Mature female llamas were assigned randomly to be treated intramuscularly (i.m.) with either 10 ml of phosphate buffered saline (PBS, negative control, n=5), 50 µg GnRH (positive control, n=5), 6 ml of llama seminal plasma (n=6) or 12 ml of bull seminal plasma (n=6). Experiment 2 was done to determine the effect of OIF in bovine seminal plasma on LH-induced ovulation and luteal development. Beef heifers with a CL and a growing follicle ≥10 mm were given a luteolytic dose of prostaglandin followed by 25 mg pLH 12 h later. Heifers were assigned randomly to three groups and given 10 ml bovine seminal plasma i.m. 12 h after pLH treatment (n=10), bovine seminal plasma i.m. within 4 h after ovulation (n=9), or no further treatment (control, n=10).Experiment 3 was done to determine the effect of OIF in bovine seminal plasma on LH release, ovulation and luteal development. Ovulation in beef heifers was synchronized using a protocol with progesterone and estradiol. Six days after ovulation, , when a mature CL and a dominant follicle of 11-13 mm diameter were expected to be present, heifers were assigned randomly to four groups (n=8 per group) using a 2-by-2 design and treated with either pLH or phosphate-buffered saline i.m., followed 12 h later by treatment with either 10 ml bovine seminal plasma or phosphate-buffered saline i.m.; i.e., LH+PBS, LH+SP, PBS+SP, and PBS+PBS groups. In all experiments, ovulation and CL development were monitored by transrectal ultrasonography. In Experiment 1, llamas were scanned daily from treatment to Day 6 after treatment, while in the other two experiments ovulations were monitored every 4 h and CL development was monitored daily until the next ovulation. Ovulation rates were compared among groups by Fisher’s exact test, and continuous data were compared among groups by ANOVA for repeated measures. Single point data were compared by ANOVA. In Experiment 1, ovulation was detected in 0/5, 4/5, 4/6, 4/6 in PBS, GnRH, llama seminal plasma, and bovine seminal plasma groups, respectively (P<0.05). No difference was detected among groups in luteal development. In Experiment 2, all ovulations in the pre-ovulation treatment group occurred within a 4 h period, while the range for other groups was 22 h (P<0.0001). No difference was detected among groups in luteal development; however, plasma progesterone concentrations tended to be greater in the heifers treated with seminal plasma post-ovulation compared to the other two groups (treatment-by-day interaction, P=0.1). In Experiment 3, ovulations were detected in 5/8, 4/8, 0/7, 0/8 in pLH,… Advisors/Committee Members: Adams, Gregg P., Mapletoft, Reuben J., Singh, Jaswant, Muir, Gillian, Barth, Albert.

Subjects/Keywords: Ovulation-inducing factor - Cattle nerve growth factor - reproduction

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APA (6^th Edition):

Tribulo, P. (2012). THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-10-766

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tribulo, Paula. “THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE.” 2012. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/ETD-2012-10-766.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tribulo, Paula. “THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE.” 2012. Web. 03 Mar 2021.

Vancouver:

Tribulo P. THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/ETD-2012-10-766.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tribulo P. THE EFFECT OF OVULATION-INDUCING FACTOR (OIF) IN BOVINE SEMINAL PLASMA ON OVARIAN FUNCTION IN CATTLE. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-10-766

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

17. Popoola, Aminat Adeola. A fiducial subject pre-alignment system for the Biomedical Imaging and Therapy beamlines at the Canadian Light Source.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-04142009-181529

► Positioning, immobilization, and organ motion are some of the major concerns in all imaging modalities. With synchrotron X-ray imaging, alignment of the region of interest… (more)

Subjects/Keywords: Pre-alignment; System; Region of Interest; Synchrotron methods; BMIT beamlines; Positioning system

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APA (6^th Edition):

Popoola, A. A. (2009). A fiducial subject pre-alignment system for the Biomedical Imaging and Therapy beamlines at the Canadian Light Source. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-04142009-181529

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Popoola, Aminat Adeola. “A fiducial subject pre-alignment system for the Biomedical Imaging and Therapy beamlines at the Canadian Light Source.” 2009. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-04142009-181529.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Popoola, Aminat Adeola. “A fiducial subject pre-alignment system for the Biomedical Imaging and Therapy beamlines at the Canadian Light Source.” 2009. Web. 03 Mar 2021.

Vancouver:

Popoola AA. A fiducial subject pre-alignment system for the Biomedical Imaging and Therapy beamlines at the Canadian Light Source. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-04142009-181529.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Popoola AA. A fiducial subject pre-alignment system for the Biomedical Imaging and Therapy beamlines at the Canadian Light Source. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-04142009-181529

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

18. Bogle, Orleigh Addelecia. Interspecies comparison of the effect of ovulation-inducing factor (OIF) in seminal plasma.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-09142009-112431

► The purpose of the studies reported in this thesis was to provide further evidence in support of the hypothesis that ovulation-inducing factor (OIF) is a… (more)

▼ The purpose of the studies reported in this thesis was to provide further evidence in support of the hypothesis that ovulation-inducing factor (OIF) is a component of seminal plasma which is conserved amongst mammals. Based on studies conducted in vivo, the results indicate that males ejaculate a substance during copulation which is responsible for the ovulatory and luteotrophic effect in female camelids. In our lab we have developed an in vivo llama bioassay to study the presence and biological effects of OIF in seminal plasma from different species. The objective of the first experiment within the first study was to determine if llama seminal plasma would stimulate ovulation in prepubertal mice. Mice were treated with a single 0.1 mL intraperitoneal dose of 1) phosphate-buffered saline (negative control), 2) 5 µg gonadotropin-releasing hormone (GnRH), 3) 5 IU of human chorionic gonadotropin (hCG) or 4) llama seminal plasma. Results indicate that prepubertal mice treated with GnRH, hCG or llama seminal plasma stimulated similar proportions of mice to ovulate, which were all higher than the proportion of mice that ovulated after saline treatment. The number of oocytes observed under a stereomicroscope was also higher in all treatment groups than in mice treated with saline. However, the number of oocytes observed was lower in mice treated with seminal plasma than those treated with GnRH, both of which were similar to the number of oocytes observed in hCG treated mice. In a second part of this study the corollary that OIF is present in the seminal plasma of horses and pigs was examined. Seminal plasma from horses or pigs was administered intramuscularly to female llamas and ovulation was monitored using transrectal ultrasonography. Llamas were treated with an intramuscular dose of 1) phosphate buffered saline (negative control), 2) llama seminal plasma (positive control), 3) equine seminal plasma or 4) porcine seminal plasma. Ovulations were detected in llamas treated with seminal plasma while none were observed in saline-treated llamas. The proportion of llamas that ovulated when treated with equine seminal plasma was higher than llamas treated with saline. The proportion of llamas that ovulated after porcine seminal plasma tended to differ from negative control groups, but did not reach statistical significance. The proportion of llamas that ovulated after equine or porcine seminal plasma treatment was lower than animals treated with llama seminal plasma which indicates that either OIF is not present in equal concentration among mammals, or that OIF is not structurally the same across mammals. The second study was carried out to test the hypothesis that OIF stimulates LH secretion at the level of the anterior pituitary gland. The second objective was to determine if the degree of LH release was related to the dose of OIF treatment. Anterior pituitary cells (2 x 10⁶ cells/ well) from either llamas (reflex ovulator) or cattle (spontaneous ovulator) were incubated for 2 hours with either media containing no… Advisors/Committee Members: Adams, Gregg P., Pierson, Roger, Singh, Baljit, Verge, Valerie.

Subjects/Keywords: ovulation-inducing factor; OIF; Ovulation; Seminal plasma; Camelid

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APA (6^th Edition):

Bogle, O. A. (2009). Interspecies comparison of the effect of ovulation-inducing factor (OIF) in seminal plasma. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-09142009-112431

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bogle, Orleigh Addelecia. “Interspecies comparison of the effect of ovulation-inducing factor (OIF) in seminal plasma.” 2009. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-09142009-112431.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bogle, Orleigh Addelecia. “Interspecies comparison of the effect of ovulation-inducing factor (OIF) in seminal plasma.” 2009. Web. 03 Mar 2021.

Vancouver:

Bogle OA. Interspecies comparison of the effect of ovulation-inducing factor (OIF) in seminal plasma. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-09142009-112431.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bogle OA. Interspecies comparison of the effect of ovulation-inducing factor (OIF) in seminal plasma. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-09142009-112431

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

19. Jaiswal, Rajesh Shriniwas. Regulation of follicular wave pattern in cattle.

Degree: 2007, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-08302007-163724

► The wave-like developmental pattern of follicles ≥1 mm in temporal relationship with follicle stimulating hormone (FSH) and the existence of 2- and 3-waves of follicular… (more)

Subjects/Keywords: FSH; progesterone; anti-GnRH; oocyte competence; wave pattern repeatability; cattle; histology; ultrasonography; Antral follicles; wave pattern; follicular dynamics

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APA (6^th Edition):

Jaiswal, R. S. (2007). Regulation of follicular wave pattern in cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08302007-163724

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jaiswal, Rajesh Shriniwas. “Regulation of follicular wave pattern in cattle.” 2007. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-08302007-163724.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jaiswal, Rajesh Shriniwas. “Regulation of follicular wave pattern in cattle.” 2007. Web. 03 Mar 2021.

Vancouver:

Jaiswal RS. Regulation of follicular wave pattern in cattle. [Internet] [Thesis]. University of Saskatchewan; 2007. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-08302007-163724.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jaiswal RS. Regulation of follicular wave pattern in cattle. [Thesis]. University of Saskatchewan; 2007. Available from: http://hdl.handle.net/10388/etd-08302007-163724

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

20. Vollmers, Antony Stanley. Calibration of ultrasound scanners for surface impedance measurement.

Degree: 2005, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-03312005-150134

► The primary objective of this research was to investigate the feasibility of calibrating ultrasound scanners to measure surface impedance from reflection data. The method proposed… (more)

▼ The primary objective of this research was to investigate the feasibility of calibrating ultrasound scanners to measure surface impedance from reflection data. The method proposed uses calibration curves from known impedance interfaces. This plot, or calibration curve, may then be used, with interpolation, to relate measured grey level to impedance for the characterization of tissue specimens with unknown properties. This approach can be used independent of different medical ultrasound scanner systems to solve for reproducible tissue impedance values without offline data processing and complicated custom electronics. Two medical ultrasound machines from different manufacturers were used in the experiment; a 30 MHz and a 7.5 MHz machine. The calibration curves for each machine were produced by imaging the interfaces of a vegetable oil floating over varying salt solutions. To test the method, porcine liver, kidney, and spleen acoustical impedances were determined by relating measured grey levels to reflection coefficients using calibration curves and then inverting the reflection coefficients to obtain impedance values. The 30 MHz ultrasound machine’s calculated tissue impedances for liver, kidney, and spleen were 1.476 ± 0.020, 1.486 ± 0.020, 1.471 ± 0.020 MRayles respectively. The 7.5 MHz machine’s tissue impedances were 1.467 ± 0.088, 1.507 ± 0.088, and 1.457 ± 0.088 MRayles respectively for liver, kidney and spleen. The differences between the two machines are 0.61%, 1.41%, and 0.95% for the impedance of liver, kidney, and spleen tissue, respectively. If the grey level is solely used to characterize the tissue, then the differences are 45.9%, 40.3%, and 39.1% for liver, kidney, and spleen between the two machines. The results support the hypothesis that tissue impedance can be determined using calibration curves and be consistent between multiple machines. Advisors/Committee Members: Dolovich, Allan T., Simonson, Carey J., Burton, Richard T., Adams, Gregg P..

Subjects/Keywords: Inverse Problem; Calibration; Tissue Characterization; Ultrasonography; Tissue Impedance

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APA (6^th Edition):

Vollmers, A. S. (2005). Calibration of ultrasound scanners for surface impedance measurement. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-03312005-150134

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vollmers, Antony Stanley. “Calibration of ultrasound scanners for surface impedance measurement.” 2005. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-03312005-150134.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vollmers, Antony Stanley. “Calibration of ultrasound scanners for surface impedance measurement.” 2005. Web. 03 Mar 2021.

Vancouver:

Vollmers AS. Calibration of ultrasound scanners for surface impedance measurement. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-03312005-150134.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vollmers AS. Calibration of ultrasound scanners for surface impedance measurement. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-03312005-150134

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

21. Dias, Fernanda Caminha Faustino. Effect of progesterone on GnRH-mediated LH release, oocyte quality, and fertility in cattle.

Degree: 2008, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-06262008-170154

► The objective was to investigate the effects of progesterone (P4) on luteinizing hormone (LH) release, follicle development, and oocyte competence in cattle. We tested the… (more)

▼ The objective was to investigate the effects of progesterone (P4) on luteinizing hormone (LH) release, follicle development, and oocyte competence in cattle. We tested the general hypotheses that: 1) The suppressive effect of P4 on gonadotrophin releasing hormone (GnRH)-mediated LH release can be overcome by increasing GnRH dose or pre-treatment with estradiol (E2); and 2) a shorter period of P4 exposure during the growing phase of the ovulatory follicle improves oocyte competence and fertility after fixed-time artificial insemination or superstimulation in cattle. In the first experiment, heifers (n=22) were treated with 100 or 200 µg of GnRH or pretreated with E2 prior to administration of GnRH during high or low circulating P4 concentrations to characterize LH release (Chapter 2). Increasing the dose of GnRH did not alter LH secretion; however, E2 pretreatment overcame the suppressive effect of high P4 on LH secretion. Cattle with lower (n=11) P4 concentrations had higher circulating LH concentrations than those with higher P4 concentrations (n=11), and tended to have higher ovulation rates. Two experiments were conducted to determine the effect of the duration of P4 exposure during the ovulatory wave on fertility followed fixed-time artificial insemination or superstimulation. In the first experiment (Chapter 3), the dominant follicle was allowed to grow for 3 days (n=181) or 6 days (n=184). Six days of growth resulted in a larger dominant follicle, but in both groups, ovulatory follicles had similar capacities to ovulate and establish pregnancy. In the second experiment (Chapter 4), multiple follicles were allowed to grow for 3 or 6 days by 8 or 14 injections of FSH (at 12-hour intervals). There was no difference between groups for ovulation rate or total ova/embryo recovery rate. Although the 3-day group had higher embryo quality at slaughter (4 days after insemination), further development (7, 9, and 10 days after insemination) did not differ among groups. The effect of FSH starvation following 4 days of FSH treatment (Chapter 4) resulted in loss of ovulatory capability. Overall, a shorter duration of P4 exposure during ovulatory follicle growth did not improve fertility after fixed-time AI or oocyte competence after superstimulation. Advisors/Committee Members: Singh, Jaswant, Singh, Baljit, Palmer, Colin, Mapletoft, Reuben J., Kastelic, John, Adams, Gregg P..

Subjects/Keywords: cattle; fertility; follicle; GnRH; LH; pregnancy rate; progesterone

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dias, F. C. F. (2008). Effect of progesterone on GnRH-mediated LH release, oocyte quality, and fertility in cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06262008-170154

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dias, Fernanda Caminha Faustino. “Effect of progesterone on GnRH-mediated LH release, oocyte quality, and fertility in cattle.” 2008. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-06262008-170154.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dias, Fernanda Caminha Faustino. “Effect of progesterone on GnRH-mediated LH release, oocyte quality, and fertility in cattle.” 2008. Web. 03 Mar 2021.

Vancouver:

Dias FCF. Effect of progesterone on GnRH-mediated LH release, oocyte quality, and fertility in cattle. [Internet] [Thesis]. University of Saskatchewan; 2008. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-06262008-170154.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dias FCF. Effect of progesterone on GnRH-mediated LH release, oocyte quality, and fertility in cattle. [Thesis]. University of Saskatchewan; 2008. Available from: http://hdl.handle.net/10388/etd-06262008-170154

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

22. McCorkell, Robert Bruce. Endogenous and exogenous control of ovarian dynamics in wapiti.

Degree: 2006, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-07062006-153821

► A series of studies were guided by the principal hypothesis that it was necessary to characterize ovarian function during the seasons of the annual reproductive… (more)

▼ A series of studies were guided by the principal hypothesis that it was necessary to characterize ovarian function during the seasons of the annual reproductive cycle in wapiti and that from this knowledge novel methods of exogenous control of ovarian function would be possible. To augment existing knowledge about endogenous control of ovarian function in wapiti studies were conducted to characterize ovarian follicle dynamics during the estrous season and to characterize ovarian follicular dynamics during the periods of transition into and out of the breeding season. The third study was designed to characterize ovarian follicle development and ovulation synchrony subsequent to an estrous synchronization protocol used commercially. To evaluate novel methods of exogenous control of ovarian function studies were conducted to determine if follicular wave emergence could be electively induced using hormonal or surgical treatments to evaluate novel ovarian superstimulatory treatment protocols. It was concluded from the studies of ovarian function that follicle development during the breeding season was characterized by the regular and synchronous development of follicular waves and that 2, 3, or 4 waves occurred during each interovulatory interval. Transition into the breeding season was preceded by one short interovulatory interval (9 days) characterized by one wave of follicle development and a small, short-lived and hypo-functional corpus luteum. The last estrous cycle of the breeding season was similar to estrous cycles during the rut (21 days), but and transition to anestrus was marked by a failure of the dominant follicle to ovulate after luteal regression. The treatment protocol used commercially for estrous synchronization was effective, but unnecessarily long. It was concluded from the studies on exogenous control of ovarian function that follicular wave emergence could be electively induced using steroid hormones or follicle ablation and may be useful for estrus synchronization and superstimulatory protocols. The tested superstimulatory treatments were effective and had the advantage of reducing the treatment period by 6 days and the number of times the animals are handled by one third over a more conventional method. However, oocyte and embryo quality were not evaluated. As a result of the studies conducted and one previous study during the anouvlatory season follicle and luteal dynamics are now known in wapiti for all seasons of the year and this knowledge will provide a template upon which other species of deer can be compared. The final two studies support the principal hypothesis. The novel methods of exogenous ovarian control tested increase the potential for success when applying reproductive technologies and the successful application of these methods in wapiti should lead to their successful use in other species of deer. Advisors/Committee Members: Adams, Gregg P., Woodbury, Murray R., Muir, Gillian D., Mapletoft, Reuben J., Flood, Peter F..

Subjects/Keywords: ovary; follicle; cervid; follicle wave; estrous synchronization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McCorkell, R. B. (2006). Endogenous and exogenous control of ovarian dynamics in wapiti. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-07062006-153821

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McCorkell, Robert Bruce. “Endogenous and exogenous control of ovarian dynamics in wapiti.” 2006. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-07062006-153821.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McCorkell, Robert Bruce. “Endogenous and exogenous control of ovarian dynamics in wapiti.” 2006. Web. 03 Mar 2021.

Vancouver:

McCorkell RB. Endogenous and exogenous control of ovarian dynamics in wapiti. [Internet] [Thesis]. University of Saskatchewan; 2006. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-07062006-153821.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McCorkell RB. Endogenous and exogenous control of ovarian dynamics in wapiti. [Thesis]. University of Saskatchewan; 2006. Available from: http://hdl.handle.net/10388/etd-07062006-153821

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

23. Yapura, Jimena. Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-08212009-094306

► Two studies were designed to characterize the effects of a non-steroidal aromatase inhibitor, letrozole, on ovarian function in cattle. The specific objective was to test… (more)

Subjects/Keywords: Ovarian follicles; Letrozole; Aromatase inhibitors; Bovine reproduction; Estrous synchronization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yapura, J. (2009). Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-08212009-094306

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yapura, Jimena. “Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle.” 2009. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-08212009-094306.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yapura, Jimena. “Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle.” 2009. Web. 03 Mar 2021.

Vancouver:

Yapura J. Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-08212009-094306.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yapura J. Effects of a non-steroidal aromatase inhibitor on ovarian function in cattle. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-08212009-094306

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

24. Cai, Wei. Equine immobilization with a limb restraint system.

Degree: 2007, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-06092007-110719

► Mobility of the horse to initiate motion from the standing position is examined in this thesis. In particular, the thesis focuses on the study of… (more)

▼ Mobility of the horse to initiate motion from the standing position is examined in this thesis. In particular, the thesis focuses on the study of the mobility of a horse with fixed hooves to the ground, and on how its musculoskeletal system is used to free the legs from restraints. Possible leg patterns to initiate motions are investigated. The breaking forces generated at front and hind hooves during static-pulling and dynamic jerking are evaluated. Design of the restraint system that uses ropes to immobilize certain joints in order to prevent the horse from generating these forces is the main objective of this thesis. Such a system could be applied as an alternative to rather massive mechanical devices, the main purpose of which is to block the breaking forces (which are quite large when fully developed). Analysis of the mobility of the horse is based on the mechanics of a skeletal linkage system driven by muscle forces. Only major muscles involved in fighting the restraints are included in the analysis. The force generation capability of a muscle is determined by physiological cross sectional area (PCSA) of the muscle. Possible leg patterns are predicted with the kinematics analysis considering range of motion at each joint in the legs. Corresponding breaking forces generated in each pattern is evaluated with the kinetics analysis. Relationship between the characteristic parameter of the pattern and the breaking force at hoof are established. The horse's computer model is used to justify the analytical result. Fighting mechanisms of the horse are simulated in the dynamic simulation software package. Patterns and the breaking forces developed by the horse model simulation agree well with the analytical results. To the author’s best knowledge, this is the first time a computer model is used in analyzing the method of restraining an animal. The mobility of the animal with hoof restraints and methods to remove mobility were further confirmed with a preliminary animal restraint test conducted on a sheep. The sheep was chosen because the leg patterns to initiate motion on a horse are similar to that of sheep, but the sheep is more convenient to handle. The experiment showed that the mobility of the sheep could be removed completely by restraining its hooves, lower legs, and head with easily attached ropes. Advisors/Committee Members: Szyszkowski, Walerian, Chapman, L. Dean, Burton, Richard T., Adams, Gregg P..

Subjects/Keywords: Joint Restraints; Breaking Force; Immobilization; Standing Horse; Mobility

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cai, W. (2007). Equine immobilization with a limb restraint system. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06092007-110719

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cai, Wei. “Equine immobilization with a limb restraint system.” 2007. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-06092007-110719.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cai, Wei. “Equine immobilization with a limb restraint system.” 2007. Web. 03 Mar 2021.

Vancouver:

Cai W. Equine immobilization with a limb restraint system. [Internet] [Thesis]. University of Saskatchewan; 2007. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-06092007-110719.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cai W. Equine immobilization with a limb restraint system. [Thesis]. University of Saskatchewan; 2007. Available from: http://hdl.handle.net/10388/etd-06092007-110719

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

25. Birtch, Rebecca Lynn. Image analysis of dominant ovarian follicles and ovarian follicular development during continuous and conventional oral contraceptive dosing schemes.

Degree: 2005, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-04302005-062845

► The objective of this research was to assess ultrasound image attributes of human dominant ovarian follicles in the final stages of development during natural and… (more)

▼ The objective of this research was to assess ultrasound image attributes of human dominant ovarian follicles in the final stages of development during natural and oral contraceptive (OC) cycles, as well as characterize ovarian follicular and endometrial development during and after continuous versus conventional dosing schemes. We utilized sophisticated computer algorithms to elucidate an association between image attributes and physiologic status of follicles in their final stage of development. We used transvaginal ultrasonography to quantify changes in the numbers and diameters of ovarian follicles and changes in endometrial thickness and pattern during and following discontinuation of two different regimens of OC. Developmental changes in ovarian follicles and corpora lutea were correlated with serum estradiol-17â and progesterone, respectively to provide a comprehensive approach to examining ovarian and uterine function. We reported for the first time that follicles which develop during natural and OC cycles have similar image attributes, which provides preliminary evidence that image attributes of human follicles are associated with physiologic status during the growth phase. Further research should be performed to elucidate the exact correlation between image attributes during all stages of follicular development throughout the menstrual cycle, prediction of dysfunctional follicular development (i.e., hemorrhagic anovulatory follicles) and the effects of different OC formulations on follicle development. Once the association between image attributes and various scenarios of follicular development are determined, a computer program could be developed to assess follicular health with a single ultrasound examination, obviating many ethical constraints that currently prevent large scale progress in ovarian follicular research. We further documented that continuous OC administration schemes provide greater follicular suppression than conventional dosing schemes. No dominant follicles developed during three consecutive 28 day cycles of continuous OC use, whereas eight dominant follicles developed during the same time period of conventional OC use. We interpreted these findings to mean that continuous OC dosing schemes provide a more effective contraceptive with a decreased risk of “escape” ovulation compared to conventional dosing schemes. Most follicles ovulated in the immediate cycle following discontinuation of OC. We suggest that the delay to fertility following cessation of OC is not due to anovulation but other yet, unknown, biological factors. Advisors/Committee Members: Pierson, Roger A., Singh, Jaswant, Ramsden, Vivian, Olatunbosun, Olufemi A., Adams, Gregg P..

Subjects/Keywords: oral contraceptive; ovarian follicles; image analysis

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APA (6^th Edition):

Birtch, R. L. (2005). Image analysis of dominant ovarian follicles and ovarian follicular development during continuous and conventional oral contraceptive dosing schemes. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-04302005-062845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Birtch, Rebecca Lynn. “Image analysis of dominant ovarian follicles and ovarian follicular development during continuous and conventional oral contraceptive dosing schemes.” 2005. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-04302005-062845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Birtch, Rebecca Lynn. “Image analysis of dominant ovarian follicles and ovarian follicular development during continuous and conventional oral contraceptive dosing schemes.” 2005. Web. 03 Mar 2021.

Vancouver:

Birtch RL. Image analysis of dominant ovarian follicles and ovarian follicular development during continuous and conventional oral contraceptive dosing schemes. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-04302005-062845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Birtch RL. Image analysis of dominant ovarian follicles and ovarian follicular development during continuous and conventional oral contraceptive dosing schemes. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-04302005-062845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

26. Bian, Na. Evaluation of texture features for analysis of ovarian follicular development.

Degree: 2005, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-12022005-114421

► Ovarian follicles in women are fluid-filled structures in the ovary that contain oocytes (eggs). A dominant follicle is physiologically selected and ovulates during the menstrual… (more)

Subjects/Keywords: trend analysis; ovarian follicle; texture analysis; feature extraction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bian, N. (2005). Evaluation of texture features for analysis of ovarian follicular development. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12022005-114421

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bian, Na. “Evaluation of texture features for analysis of ovarian follicular development.” 2005. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-12022005-114421.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bian, Na. “Evaluation of texture features for analysis of ovarian follicular development.” 2005. Web. 03 Mar 2021.

Vancouver:

Bian N. Evaluation of texture features for analysis of ovarian follicular development. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-12022005-114421.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bian N. Evaluation of texture features for analysis of ovarian follicular development. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-12022005-114421

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

27. Baerwald, Angela Renee. Human ovarian follicular dynamics during natural menstrual cycles and oral contraception cycles.

Degree: 2003, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-07252008-083010

► The objective of the research comprising this thesis was to characterize ovarian follicular development in healthy women of reproductive age undergoing natural menstrual cycles and… (more)

▼ The objective of the research comprising this thesis was to characterize ovarian follicular development in healthy women of reproductive age undergoing natural menstrual cycles and oral contraception (DC) cycles. We quantified changes in the numbers and diameters of follicles, detected ovulation and assessed changes in the growth and regression of corpora lutea using high-resolution transvaginal ultrasonography. Changes in follicular and luteal development were then correlated with changes in concentrations of reproductively-active hormones and endometrial growth to provide a comprehensive approach to ovarian and uterine function. We documented, for the first time, that women exhibited waves of antral follicular development during the menstrual cycle. Two and three waves of follicle growth were observed. Major and minor waves of follicle development were characterized. Major waves were those in which a dominant follicle was selected for preferential growth; minor waves were those in which dominance was not manifest. Luteal progesterone production appeared to have a negative effect on the emergence and development of follicle waves in women. The ovarian follicular wave phenomenon has provided a new model for studying the growth and regression of ovarian follicles during the human menstrual cycle. Documentation of ovarian follicular waves in women has implications for the development of new strategies to manipulate ovarian follicular development, in particular hormonal contraceptive regimens and infertility therapies. We further documented that ovarian follicular development occurred during the compliant use of oral contraception. Follicles developed to ostensibly ovulatory diameters, and either regressed, ovulated or formed follicular cysts under the suppressive effects of DC. The majority of follicles that developed during DC use emerged during the hormone-free interval (HFI). We interpreted our findings to mean that ovarian follicular development during DC use was associated with loss of endocrine suppression during the HFI, rather than user non-compliance as previously speculated. The number and maximum diameter of follicles that developed during DC use were greater in women administered DC containing 20 µg versus 30-35 µg Ethinyl Estradiol formulations. Our results provided rationale for a reduction or complete elimination of the HFI in OC regimens, and the judicious use of low EE dose OC regimens (ie., 10 mm). Our findings demonstrated that dominant follicles secrete estradiol and become increasingly responsive to LH as they acquire functional dominance after becoming physiologically selected for preferential growth during the follicular phase of the menstrual cycle. Advisors/Committee Members: Olatunbosun, Olufemi A., Mapletoft, Reuben J., Chizen, Donna, Case, Allison, Adams, Gregg P..

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baerwald, A. R. (2003). Human ovarian follicular dynamics during natural menstrual cycles and oral contraception cycles. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-07252008-083010

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baerwald, Angela Renee. “Human ovarian follicular dynamics during natural menstrual cycles and oral contraception cycles.” 2003. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-07252008-083010.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baerwald, Angela Renee. “Human ovarian follicular dynamics during natural menstrual cycles and oral contraception cycles.” 2003. Web. 03 Mar 2021.

Vancouver:

Baerwald AR. Human ovarian follicular dynamics during natural menstrual cycles and oral contraception cycles. [Internet] [Thesis]. University of Saskatchewan; 2003. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-07252008-083010.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baerwald AR. Human ovarian follicular dynamics during natural menstrual cycles and oral contraception cycles. [Thesis]. University of Saskatchewan; 2003. Available from: http://hdl.handle.net/10388/etd-07252008-083010

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

28. Martand#237;nez, Marcelo Fabiand#225. Synchronization of follicular wave dynamics and ovulation for fixed-time artificial insemination in cattle.

Degree: 2002, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-12182008-081636

► The overall objective was to develop new synchronization protocols that facilitate fixed-time artificial insemination (AI). A series of experiments were designed to evaluate the effects… (more)

▼ The overall objective was to develop new synchronization protocols that facilitate fixed-time artificial insemination (AI). A series of experiments were designed to evaluate the effects of estradiol and progesterone on gonadotrophin release, follicular wave emergence and ovulation in beef cattle. In ovariectomized cows, a new CIDR-B device increased plasma progesterone to near-luteal concentrations, but for only 2 to 3 days. Injection of 100 mg progesterone increased plasma progesterone approximately 2 ng/mL. Progesterone suppressed plasma LH concentrations but did not affect plasma FSH concentrations. Estradiol, with or without progesterone, resulted in FSH suppression with resurgence (and follicular wave emergence) at an interval that varied according to the estradiol formulation. Estradiol administration following CIDR-B removal resulted in LH release (and ovulation in intact animals). Both estradiol-17Î² and estradiol benzoate (EB) synchronized ovarian follicular wave emergence in CIDR-Bï¿½-treated animals and the interval from CIDR-B removal to ovulation (72 to 120 h) was shorter and more synchronous in estradiol-treated animals than in controls. In cattle given a CIDR-B device and estradiol plus progesterone, estradiol treatment following CIDR-B removal 7 days later resulted in acceptable conception rates to fixed-time AI. Estradiol or GnRH at the beginning and end of a 7-day MGA-based synchronization regimen resulted in acceptable pregnancy rates to fixed-time AI. In a single experiment, EB, GnRH or pLH in CIDR-B- or MGA-treated beef heifers effectively synchronized ovulation for fixed-time AI. Pregnancy rates were, on average, 58.0% (range 52.5 to 65.0%). A 6- or 7-d interval from GnRH to PGF in a Cosynch regimen resulted in similar pregnancy rates in cows. The addition of a progestin to a Cosynch or Ovsynch regimen improved pregnancy rates in heifers but not in cows. Synchronization of follicular wave emergence and ovulation in a two-dose PGF-based protocol resulted in acceptable fertility to fixed-time AI; the administration of EB induced luteal regression in some animals but E-17Î² did not. In conclusion, synchronization programs including GnRH, pLH or estradiol to synchronize follicular wave emergence and ovulation in CIDR-B-, MGA- or two-dose PGF-protocols resulted in acceptable pregnancy rates to fixed-time AI. Advisors/Committee Members: Mapletoft, Reuben J., Kastelic, John, Adams, Gregg P., Pierson, Roger A..

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APA (6^th Edition):

Martand#237;nez, M. F. (2002). Synchronization of follicular wave dynamics and ovulation for fixed-time artificial insemination in cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12182008-081636

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Martand#237;nez, Marcelo Fabiand#225. “Synchronization of follicular wave dynamics and ovulation for fixed-time artificial insemination in cattle.” 2002. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-12182008-081636.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Martand#237;nez, Marcelo Fabiand#225. “Synchronization of follicular wave dynamics and ovulation for fixed-time artificial insemination in cattle.” 2002. Web. 03 Mar 2021.

Vancouver:

Martand#237;nez MF. Synchronization of follicular wave dynamics and ovulation for fixed-time artificial insemination in cattle. [Internet] [Thesis]. University of Saskatchewan; 2002. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-12182008-081636.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Martand#237;nez MF. Synchronization of follicular wave dynamics and ovulation for fixed-time artificial insemination in cattle. [Thesis]. University of Saskatchewan; 2002. Available from: http://hdl.handle.net/10388/etd-12182008-081636

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

29. Colazo, Marcos German. Use of steroid hormones or GnRH to synchronize and resynchronize follicular wave emergence, estrus, and ovulation in cattle.

Degree: 2005, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-04192005-094845

► A series of experiments were designed to study alternative estrus synchronization and resynchronization protocols to facilitate the use of artificial insemination in cattle. Studies were… (more)

▼ A series of experiments were designed to study alternative estrus synchronization and resynchronization protocols to facilitate the use of artificial insemination in cattle. Studies were conducted to study the effects of estradiol cypionate (ECP) on follicular dynamics, time of ovulation, and pregnancy rate to timed-AI (TAI) in CIDR-based protocols. Although administration of 1 mg ECP did not result in synchronous follicular wave emergence, a dose of 0.5 mg ECP synchronized LH release and ovulation. Administration of ECP 24 h after CIDR removal resulted in acceptable pregnancy rate. However, treatment with ECP at CIDR removal resulted in acceptable pregnancy rate only if follicular wave emergence was synchronized with estradiol-17â (E-17â). The efficacy of two estradiol preparations (5 mg of E-17â or estadiol valerate; EV) and reduced doses of EV on CL and ovarian follicular dynamics and superovulatory response were examined. When doses of 5 mg were compared, EV treatment resulted in a more variable interval to follicular wave emergence and a lower superovulatory response than E-17â. However, EV at a dose of 1 or 2 mg was efficacious in synchronizing follicle wave emergence in CIDR-treated cattle.Pregnancy rates were compared following TAI in cattle given a new or previously used CIDR and injections of estradiol, with or without progesterone, to synchronize follicular wave emergence. Pregnancy rate following TAI did not differ between cattle treated with a new or once-used CIDR, but pregnancy rate was lower in cattle treated with one or two twice-used CIDR. The addition of an injection of progesterone to the estradiol treatment at CIDR insertion did not enhance pregnancy rate. The efficacy of progestins (used CIDR and MGA), and E-17â, ECP, GnRH, or progesterone treatment for resynchronization of estrus in cattle not pregnant following TAI were investigated. Progestin treatment resulted in the majority of nonpregnant heifers detected in estrus over a 4-d interval. Conception rates were higher in heifers resynchronized with a once-used CIDR than with MGA. GnRH at CIDR insertion synchronized follicular wave emergence in cows, but did not increase conception rate in heifers. E-17â at CIDR insertion (1.5 mg) and removal (0.5 mg) resulted in decreased pregnancy rate following TAI. In summary, protocols described in this thesis resulted in acceptable pregnancy rates following TAI and resynchronization of previously inseminated heifers with progestins resulted in variable estrus and pregnancy rates. Advisors/Committee Members: Mapletoft, Reuben J., Pierson, Roger A., Kastelic, John, Carruthers, Terry D., Adams, Gregg P..

Subjects/Keywords: cattle; estrus; follicular wave; ovulation; timed-AI

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Colazo, M. G. (2005). Use of steroid hormones or GnRH to synchronize and resynchronize follicular wave emergence, estrus, and ovulation in cattle. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-04192005-094845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Colazo, Marcos German. “Use of steroid hormones or GnRH to synchronize and resynchronize follicular wave emergence, estrus, and ovulation in cattle.” 2005. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-04192005-094845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Colazo, Marcos German. “Use of steroid hormones or GnRH to synchronize and resynchronize follicular wave emergence, estrus, and ovulation in cattle.” 2005. Web. 03 Mar 2021.

Vancouver:

Colazo MG. Use of steroid hormones or GnRH to synchronize and resynchronize follicular wave emergence, estrus, and ovulation in cattle. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-04192005-094845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Colazo MG. Use of steroid hormones or GnRH to synchronize and resynchronize follicular wave emergence, estrus, and ovulation in cattle. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-04192005-094845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

30. Malhi, Pritpal Singh. A bovine model to study reproductive aging.

Degree: 2007, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-06042007-170036

► Decline in fertility with age has been well documented in women. There are ethical limitations to use humans as a model for basic research, and… (more)

Subjects/Keywords: ovary; reproduction; aging; bovine model; oocyte

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Malhi, P. S. (2007). A bovine model to study reproductive aging. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06042007-170036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Malhi, Pritpal Singh. “A bovine model to study reproductive aging.” 2007. Thesis, University of Saskatchewan. Accessed March 03, 2021. http://hdl.handle.net/10388/etd-06042007-170036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Malhi, Pritpal Singh. “A bovine model to study reproductive aging.” 2007. Web. 03 Mar 2021.

Vancouver:

Malhi PS. A bovine model to study reproductive aging. [Internet] [Thesis]. University of Saskatchewan; 2007. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10388/etd-06042007-170036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Malhi PS. A bovine model to study reproductive aging. [Thesis]. University of Saskatchewan; 2007. Available from: http://hdl.handle.net/10388/etd-06042007-170036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations