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You searched for +publisher:"University of Pretoria" +contributor:("Snyman, Jacques Rene"). Showing records 1 – 2 of 2 total matches.

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University of Pretoria

1. Gandy, Justin John. The medicinal properties and applications of a carbohydrate derived fulvic acid.

Degree: PhD, Pharmacology, 2015, University of Pretoria

Background: Carbohydrate derived fulvic acid (CHD-FA) is a synthetic heavy metal free fulvic acid. Although CHD-FA has been suggested as a nutritional supplement and even a medication, there is currently no data available with regards to the systemic kinetics of CHDFA when ingested orally. Fulvic acid has shown equivalence when compared to diclofenac sodium and betamethasone in a murine hypersensitivity model when applied topically with no adverse side effects. Data from several animal studies indicate that fulvic acid administered topically or orally is safe and effective as an anti-inflammatory agent. Human in vivo safety and efficacy as well as potential in vitro genotoxicity still need to be assessed. Aim: To determine whether carbohydrate derived fulvic acid shows any genotoxic effects and whether it is safe, clinically effective as an anti-inflammatory and attempt to establish a suitable CHD-FA marker for kinetic studies. Methods: Genotoxicity was determined via an in vitro micronucleus assay. Systemic and topical safety and efficacy of CHD-FA was established via two limited participant, double blind, randomised, placebo controlled clinical trials. One to determine safety and efficacy in 30 adult male volunteers with a predetermined atopic hypersensitivity, using a skin prick test, and the second trial to test topical safety and efficacy in 40 patients suffering from atopic dermatitis. LC-MS/MS assays were conducted in order to determine whether a unique or dominant ionic analyte could be found in plasma. Discussion: In the in vitro genotoxicity tests, CHD-FA compared closely to that of the negative control with respect to the number of micronuclei observed. All tested in vivo safety parameters proved to remain constant throughout both the clinical trials. A significant decrease in flare was observed in CHD-FA treated patients following a skin prick challenge. Due to the complexity of the mass spectral fingerprints of both plasma and the fulvic acid no suitable CHD-FA markers were found. Conclusion: No genotoxicity was observed for CHD-FA treated cells. No severe adverse events occurred in either the oral or topically administered CHD-FA trials, proving CHD-FA to be systemically and topically safe. A significant decrease in wheal formation in the skin prick test, and a significant clinical improvement in atopic dermatitis compared to placebo were observed implicating that CHD-FA may act as an anti-inflammatory agent in vivo in humans. No suitable CHD-FA markers for pharmacokinetic studies in human plasma could be identified. Advisors/Committee Members: Cromarty, Allan Duncan (advisor), Medlen, C.E. (coadvisor), Snyman, Jacques Rene (coadvisor).

Subjects/Keywords: UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Gandy, J. J. (2015). The medicinal properties and applications of a carbohydrate derived fulvic acid. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/56957

Chicago Manual of Style (16th Edition):

Gandy, Justin John. “The medicinal properties and applications of a carbohydrate derived fulvic acid.” 2015. Doctoral Dissertation, University of Pretoria. Accessed September 25, 2020. http://hdl.handle.net/2263/56957.

MLA Handbook (7th Edition):

Gandy, Justin John. “The medicinal properties and applications of a carbohydrate derived fulvic acid.” 2015. Web. 25 Sep 2020.

Vancouver:

Gandy JJ. The medicinal properties and applications of a carbohydrate derived fulvic acid. [Internet] [Doctoral dissertation]. University of Pretoria; 2015. [cited 2020 Sep 25]. Available from: http://hdl.handle.net/2263/56957.

Council of Science Editors:

Gandy JJ. The medicinal properties and applications of a carbohydrate derived fulvic acid. [Doctoral Dissertation]. University of Pretoria; 2015. Available from: http://hdl.handle.net/2263/56957


University of Pretoria

2. Laurens, Ilze. Development of a new extraction method for platelet-rich plasma and partial purification of platelet-derived growth factor and transforming growth factor beta.

Degree: Pharmacology, 2013, University of Pretoria

Platelet-rich plasma (PRP) is the cell free plasma, which has an enriched concentration of platelets and clotting factors with the ability to enhance the natural healing process. PRP is often used by physicians in an office setting to accelerate the healing of a variety of sports related injuries, chronic wounds and enhance skin rejuvenation. PRP mimics the wound healing cascade by enhancing the recruitment, proliferation and differentiation of cells involved in tissue regeneration. Although PRP is used to enhance healing, the efficacy thereof is debated as no clear-cut set of parameters is available that device manufacturers and protocols should follow. The lack of uniformity in the PRP preparation methods results in differing PRP volume, platelet contents and unavoidably platelet-derived growth factors. Therefore, the aim of this study was to develop a simple and rapid method for preparing autologous PRP in an office setting using a tabletop centrifuge for point-of-care use. The simplified preparation procedure involved a single centrifugation step of 18 ml of whole blood, which sufficiently enriched the platelet content in the PRP fraction. As activated platelets express and release growth factors and cytokines that mediate the different phases of the wound healing cascade, the extracted PRP fraction was activated with an ethanol, calcium chloride (CaCl2) and platelet poor plasma (PPP) preparation in glass containers, without the collection of additional blood as required in some protocols. The activated PRP formed a fibrin clot, trapping the degranulating platelets and its released growth factors. The concentration of TGF- 1 obtained from the fibrin clot was 45.49 ± 3.80 ng/ml, in range with the available literature. During the in vitro studies, the extracted PRP by the developed method was able to significantly induce cell proliferation in a dose dependent manner. Cells enumerated with the crystal violet assay indicated that the cells treaded with 5% or 10% PRP significantly increased the percentage of viable cells to 165-176% and 156-158%, when compared to the positive controls. Cells enumerated with the MTT-assay indicated that the cells treaded with 5% or 10% PRP increased the percentage of viable cells to 79-91% and 87-105% which is comparable to that of the positive control. Data from the cellular proliferation assays indicate that sufficient plateletderived growth factors had been obtained with the preparation procedure. Furthermore, data from the in vivo studies indicated that the extracted PRP was able to augment soft tissue regeneration and bone formation. Treatment with the activated PRP resulted in symptom reduction and accelerated healing of various injuries. The simplified preparation and the use of the provided study product packaged in a kit developed during this study will enable physicians to easily obtain autologous PRP, in an office setting for point-of-care use, with the ability to induce tissue regeneration. Advisors/Committee Members: Cromarty, Allan Duncan (advisor), Snyman, Jacques Rene (coadvisor), Duneas, Nicolaas (coadvisor).

Subjects/Keywords: Platelet-rich plasma (PRP); Clotting factors; Cell free plasma; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Laurens, I. (2013). Development of a new extraction method for platelet-rich plasma and partial purification of platelet-derived growth factor and transforming growth factor beta. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/40717

Chicago Manual of Style (16th Edition):

Laurens, Ilze. “Development of a new extraction method for platelet-rich plasma and partial purification of platelet-derived growth factor and transforming growth factor beta.” 2013. Masters Thesis, University of Pretoria. Accessed September 25, 2020. http://hdl.handle.net/2263/40717.

MLA Handbook (7th Edition):

Laurens, Ilze. “Development of a new extraction method for platelet-rich plasma and partial purification of platelet-derived growth factor and transforming growth factor beta.” 2013. Web. 25 Sep 2020.

Vancouver:

Laurens I. Development of a new extraction method for platelet-rich plasma and partial purification of platelet-derived growth factor and transforming growth factor beta. [Internet] [Masters thesis]. University of Pretoria; 2013. [cited 2020 Sep 25]. Available from: http://hdl.handle.net/2263/40717.

Council of Science Editors:

Laurens I. Development of a new extraction method for platelet-rich plasma and partial purification of platelet-derived growth factor and transforming growth factor beta. [Masters Thesis]. University of Pretoria; 2013. Available from: http://hdl.handle.net/2263/40717

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