Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for +publisher:"University of Notre Dame" +contributor:("Dr. Siyuan Zhang, Committee Member"). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of Notre Dame

1. Cassandra Buchheit. Elucidating cell death mechanisms in extracellular matrix-detached epithelial cells.

Degree: PhD, Biological Sciences, 2015, University of Notre Dame

When mammary epithelial cells become detached from the extracellular matrix (ECM), they undergo programmed cell death. Over the past twenty years, our knowledge regarding various mechanisms utilized by cancerous epithelial cells to overcome ECM-detachment-induced death has grown. Elucidating the ways in which cancer cells overcome ECM-detachment-induced cell death is important for our understanding of the metastatic progression and for our ability to therapeutically eliminate these cells. Here, we describe a novel mechanism utilized by inflammatory breast cancer (IBC) cells to overcome ECM-detachment-induced apoptosis, or anoikis. IBC cells were shown to survive in ECM-detached conditions by regulating the intracellular localization of the proapoptotic protein, Bim-EL via protein interactions with Beclin-1 and LC8. These data are the first to describe how IBC cells can survive in an ECM-detached state. Interestingly, inhibition of anoikis alone is not enough to overcome ECM-detachment-induced cell death in nontumorigenic mammary epithelial cells. We have identified Rip1 as a critical mediator of non-apoptotic ECM-detachment-induced cell death, which we believe to be programmed necrosis. Rip1 was found to be highly expressed in ECM-detached epithelial cells via upregulation of the deubiquitinase, CYLD. Surprisingly, Rip3 and MLKL were found to be dispensable downstream of Rip1 for ECM-detachment-induced programmed necrosis. Furthermore, TNF╬▒ does not appear to represent the stimulation factor for ECM-detachment-induced death. Together these data suggest a novel Rip1-mediated, Rip3/MLKL-independent signaling cascade for ECM-detachment-induced necrosis. Advisors/Committee Members: Dr. Siyuan Zhang, Committee Member, Dr. Rebecca Wingert, Committee Member, Dr. Crislyn DSouza-Schorey, Committee Member, Dr. Zachary Schafer, Committee Chair.

Subjects/Keywords: inflammatory breast cancer; necrosis; anoikis

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Buchheit, C. (2015). Elucidating cell death mechanisms in extracellular matrix-detached epithelial cells. (Doctoral Dissertation). University of Notre Dame. Retrieved from https://curate.nd.edu/show/wp988g87473

Chicago Manual of Style (16th Edition):

Buchheit, Cassandra. “Elucidating cell death mechanisms in extracellular matrix-detached epithelial cells.” 2015. Doctoral Dissertation, University of Notre Dame. Accessed November 22, 2017. https://curate.nd.edu/show/wp988g87473.

MLA Handbook (7th Edition):

Buchheit, Cassandra. “Elucidating cell death mechanisms in extracellular matrix-detached epithelial cells.” 2015. Web. 22 Nov 2017.

Vancouver:

Buchheit C. Elucidating cell death mechanisms in extracellular matrix-detached epithelial cells. [Internet] [Doctoral dissertation]. University of Notre Dame; 2015. [cited 2017 Nov 22]. Available from: https://curate.nd.edu/show/wp988g87473.

Council of Science Editors:

Buchheit C. Elucidating cell death mechanisms in extracellular matrix-detached epithelial cells. [Doctoral Dissertation]. University of Notre Dame; 2015. Available from: https://curate.nd.edu/show/wp988g87473

.