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You searched for +publisher:"University of North Texas" +contributor:("Nagaoka, Lisa A."). One record found.

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University of North Texas

1. Barker, Andrew L. Archaeological Proteomics: Method Development and Analysis of Protein-Ceramic Binding.

Degree: 2010, University of North Texas

The analysis of protein residues recovered from archaeological artifacts provides a unique opportunity to reveal new information about past societies. However, many scientists are currently unwilling to accept protein-based results due to problems in method development and a basic lack of agreement regarding the ability of proteins to bind to, and preserve within, artifacts such as pottery. In this paper, I address these challenges by conducting a two-phase experiment. First, I quantitatively evaluate the tendency of proteins to sorb to ceramic matrices by using total organic carbon analysis and spectrophotometric assays to analyze samples of experimentally cooked ceramic. I then test a series of solvent and physical parameters in order to develop an optimized method for extracting and preparing protein residues for identification via mass spectrometry. Results demonstrate that protein strongly sorbs to ceramic and is not easily removed, despite repeated washing, unless an appropriate extraction strategy is used. This has implications for the future of paleodietary, conservation ecology and forensic research in that it suggests the potential for recovery of aged or even ancient proteins from ceramic matrices. Advisors/Committee Members: Wolverton, Steven J., Venables, Barney J., Ferring, C. Reid, Nagaoka, Lisa A., Dandekar, Sushama.

Subjects/Keywords: Archaeological residues; protein-ceramic interaction; protein extraction; Ceramics.; Protein binding.; Proteomics.; Archaeological chemistry.; Archaeology  – Methodology.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Barker, A. L. (2010). Archaeological Proteomics: Method Development and Analysis of Protein-Ceramic Binding. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc28392/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Barker, Andrew L. “Archaeological Proteomics: Method Development and Analysis of Protein-Ceramic Binding.” 2010. Thesis, University of North Texas. Accessed June 17, 2019. https://digital.library.unt.edu/ark:/67531/metadc28392/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Barker, Andrew L. “Archaeological Proteomics: Method Development and Analysis of Protein-Ceramic Binding.” 2010. Web. 17 Jun 2019.

Vancouver:

Barker AL. Archaeological Proteomics: Method Development and Analysis of Protein-Ceramic Binding. [Internet] [Thesis]. University of North Texas; 2010. [cited 2019 Jun 17]. Available from: https://digital.library.unt.edu/ark:/67531/metadc28392/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Barker AL. Archaeological Proteomics: Method Development and Analysis of Protein-Ceramic Binding. [Thesis]. University of North Texas; 2010. Available from: https://digital.library.unt.edu/ark:/67531/metadc28392/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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