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You searched for +publisher:"University of North Carolina" +contributor:("Zaharoff, David"). One record found.

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University of North Carolina

1. Nguyen, Khue. Effect of Heparin on the Immunobiology of Interleukin-12.

Degree: Microbiology and Immunology, 2018, University of North Carolina

IL-12 is a potent pro-inflammatory cytokine that plays a central role in cellular immunity. Recently, we have shown that IL-12 is a specific heparin-binding protein. However, the interaction of heparin and IL-12 at the molecular level and the mechanisms by which heparin modulates the biological functions of IL-12 are unknown. For the first time, we described the unique ability of heparin to enhance the bioactivity of IL-12. Heparin was found to enhance binding of IL-12 to cell surfaces while modestly protecting the cytokine from proteolytic degradation. An exploration of critical heparin characteristics revealed that IL-12 binding and activity increased were dependent on heparin chain length and sulfation level. Based on our bioactivity data, we developed a model of stabilization showing that heparin likely serves as a co-receptor that enhances the interaction of IL-12 with its subunit receptors. Finally, we examined the effect of heparin on IL-12 bioactivity in IL-12RB1 deficient cells. In both PBMCs isolated from patients of Mendelian susceptibility to mycobacterial diseases (MSMD) and IL-12RB1 mutant NK-92MI cell line, heparin was found to partially recover IL-12 signaling as measured via IFN-gamma production. Taken together, these studies define a new role for heparin as a modulator of the immunobiology of IL-12 and potentially other IL-12 family cytokines. Advisors/Committee Members: Nguyen, Khue, Tisch, Roland, Zaharoff, David, Whitmire, Jason, Liu, Jian, Wan, Yisong.

Subjects/Keywords: School of Medicine; Department of Microbiology and Immunology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Nguyen, K. (2018). Effect of Heparin on the Immunobiology of Interleukin-12. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:44502cdb-4717-4c9c-a143-b0e64eeb17f4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Nguyen, Khue. “Effect of Heparin on the Immunobiology of Interleukin-12.” 2018. Thesis, University of North Carolina. Accessed November 27, 2020. https://cdr.lib.unc.edu/record/uuid:44502cdb-4717-4c9c-a143-b0e64eeb17f4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Nguyen, Khue. “Effect of Heparin on the Immunobiology of Interleukin-12.” 2018. Web. 27 Nov 2020.

Vancouver:

Nguyen K. Effect of Heparin on the Immunobiology of Interleukin-12. [Internet] [Thesis]. University of North Carolina; 2018. [cited 2020 Nov 27]. Available from: https://cdr.lib.unc.edu/record/uuid:44502cdb-4717-4c9c-a143-b0e64eeb17f4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nguyen K. Effect of Heparin on the Immunobiology of Interleukin-12. [Thesis]. University of North Carolina; 2018. Available from: https://cdr.lib.unc.edu/record/uuid:44502cdb-4717-4c9c-a143-b0e64eeb17f4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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