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You searched for +publisher:"University of North Carolina" +contributor:("White, Laura"). Showing records 1 – 2 of 2 total matches.

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University of North Carolina

1. Khalil, Syed Muaz. Characterization of Alphavirus Replicon Based Vaccines and Adjuvants in a Neonatal Murine Model.

Degree: Microbiology and Immunology, 2014, University of North Carolina

Infectious diseases are the leading cause of childhood morbidity and mortality worldwide. Not only is there a lack of vaccines for many deadly diseases, early life immunization with the available vaccines is frequently ineffective and requires multiple doses to induce protective immune response. Additionally, the neonatal immune responses to infection and vaccination are biased towards TH2 at the cost of pro-inflammatory TH1 responses needed to combat intracellular pathogens. However, upon appropriate stimulation, the neonatal immune system can induce adult-like TH1 responses. We hypothesized that non-propagating Venezuelan equine encephalitis virus replicon (VEE)-based vaccine platforms (VRP) are good candidates for safe early life immunization, based on their ability to (1) target dendritic cells (DC) in the draining lymph node and (2) induction of robust innate immune response through intracellular amplification of replicon RNA. In the VRP expression vector, the same replicon particle functions to deliver the antigen and to serve as innate immune-stimulant. On the other hand, in the VRP as adjuvant (GVI3000), the replicon particle only provides the danger signal function, while the antigen is not expressed from the replicon particle, but co-delivered as purified protein or inactivated virions. By separating the antigen delivery function from the adjuvant function in the GVI3000, we were able to demonstrate the role of the adjuvant function in the VRP, and to start to understand how it mediates the changes in the quality and magnitude of the immune response in the neonate. In this thesis, using a neonatal mouse model, we showed that both platforms induced effective and protective immunity against two different infectious agents, influenza virus and dengue virus. Furthermore, VRP replicon particles served as useful tools to better understand the induction of the neonatal immune response. In summary, the VRP expression vector vaccines and adjuvants induced an adult-like adjuvant effect against dengue influenza antigens, and have the potential to improve the immunogenicity and protective efficacy of new and existing neonatal vaccines. Advisors/Committee Members: Khalil, Syed Muaz, White, Laura, Damania, Blossom, Heise, Mark, Swanstrom, Ronald, Abel, Kristina, Whitmire, Jason.

Subjects/Keywords: Virology; Immunology; Microbiology; School of Medicine; Department of Microbiology and Immunology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Khalil, S. M. (2014). Characterization of Alphavirus Replicon Based Vaccines and Adjuvants in a Neonatal Murine Model. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:0d1cce3c-edf9-44b4-85f0-37f720d05f87

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Khalil, Syed Muaz. “Characterization of Alphavirus Replicon Based Vaccines and Adjuvants in a Neonatal Murine Model.” 2014. Thesis, University of North Carolina. Accessed December 04, 2020. https://cdr.lib.unc.edu/record/uuid:0d1cce3c-edf9-44b4-85f0-37f720d05f87.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Khalil, Syed Muaz. “Characterization of Alphavirus Replicon Based Vaccines and Adjuvants in a Neonatal Murine Model.” 2014. Web. 04 Dec 2020.

Vancouver:

Khalil SM. Characterization of Alphavirus Replicon Based Vaccines and Adjuvants in a Neonatal Murine Model. [Internet] [Thesis]. University of North Carolina; 2014. [cited 2020 Dec 04]. Available from: https://cdr.lib.unc.edu/record/uuid:0d1cce3c-edf9-44b4-85f0-37f720d05f87.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khalil SM. Characterization of Alphavirus Replicon Based Vaccines and Adjuvants in a Neonatal Murine Model. [Thesis]. University of North Carolina; 2014. Available from: https://cdr.lib.unc.edu/record/uuid:0d1cce3c-edf9-44b4-85f0-37f720d05f87

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of North Carolina

2. Webb, Drue Laine. Development of Improved Dengue 2 Antigen Configurations for a Venezuelan Equine Encephalitis Virus Replicon Particle-Launched Dengue Vaccine.

Degree: Microbiology and Immunology, 2008, University of North Carolina

Dengue has become one of the most important vector-borne diseases. There is currently no licensed dengue vaccine. The envelope glycoprotein is the major target of neutralizing antibodies. Researchers have tried to identify the most antigenic configuration of the envelope protein in different vaccine platforms. In this study, six dengue 2 E configurations, including full-length E, truncated forms of E and the domain III subunit, were expressed as antigen using the Venezuelan equine encephalitis virus replicon paricle system and characterized in vitro and in vivo. Each construct was immunogenic in mice; however, there was a correlation between protein secretion and neutralizing responses. Two truncated and soluble configurations of E encoding amino acid changes from the previously evaluated DEN2 prME-VRP sequence elicited significant neutralizing immune responses. These amino acid changes may be important in the structure of neutralizing epitopes or for proper folding, processing and secretion, leading to improved neutralizing antibody responses. Advisors/Committee Members: Webb, Drue Laine, White, Laura.

Subjects/Keywords: School of Medicine; Department of Microbiology and Immunology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Webb, D. L. (2008). Development of Improved Dengue 2 Antigen Configurations for a Venezuelan Equine Encephalitis Virus Replicon Particle-Launched Dengue Vaccine. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:f8c283c1-df19-4ff1-a62a-e44dd179459b

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Webb, Drue Laine. “Development of Improved Dengue 2 Antigen Configurations for a Venezuelan Equine Encephalitis Virus Replicon Particle-Launched Dengue Vaccine.” 2008. Thesis, University of North Carolina. Accessed December 04, 2020. https://cdr.lib.unc.edu/record/uuid:f8c283c1-df19-4ff1-a62a-e44dd179459b.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Webb, Drue Laine. “Development of Improved Dengue 2 Antigen Configurations for a Venezuelan Equine Encephalitis Virus Replicon Particle-Launched Dengue Vaccine.” 2008. Web. 04 Dec 2020.

Vancouver:

Webb DL. Development of Improved Dengue 2 Antigen Configurations for a Venezuelan Equine Encephalitis Virus Replicon Particle-Launched Dengue Vaccine. [Internet] [Thesis]. University of North Carolina; 2008. [cited 2020 Dec 04]. Available from: https://cdr.lib.unc.edu/record/uuid:f8c283c1-df19-4ff1-a62a-e44dd179459b.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Webb DL. Development of Improved Dengue 2 Antigen Configurations for a Venezuelan Equine Encephalitis Virus Replicon Particle-Launched Dengue Vaccine. [Thesis]. University of North Carolina; 2008. Available from: https://cdr.lib.unc.edu/record/uuid:f8c283c1-df19-4ff1-a62a-e44dd179459b

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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