Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for +publisher:"University of North Carolina" +contributor:("Waters, Crystal"). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of North Carolina

1. Waters, Crystal. Factors increasing the flexibility of nonhomologous end joining.

Degree: 2015, University of North Carolina

Broken chromosomes can be catastrophic for an organism if misrepaired or left unrepaired. Mammals rely predominately on non-homologous end joining (NHEJ) to efficiently repair chromosome breaks, which arise as programmed intermediates to cellular processes such V(D)J recombination or arise due to cellular damage. These sources can generate a variety of end structures often lacking sequence complementarity or containing nucleotide damage. I determined that NHEJ makes use of low fidelity direct ligation to bypass subtle mispairs and radiomimetic damage. This allows cells to avoid the consequences of an unrepaired double strand break, while allowing other repair pathways to subsequently fix the retained damage. In cases where direct ligation is impeded, NHEJ employs end-processing factors (e.g. polymerases and nucleases) to modify ends until they are ligatable. I found that end processing proceeds nonrandomly and is driven largely by the initial end structure. I found that the gaps created by aligning the ends were filled by DNA Pol λ and Pol μ, making them critical for the repair of incompatible ends. The two polymerases were found to have distinct activities and to be preferentially active on distinct substrates. Interestingly, I found that these polymerases also have a key role nuclease-dependent resolution by NHEJ. This work has shown that NHEJ systematically adapts to different end structures, which demonstrates just how sophisticated and flexible this pathway is. Advisors/Committee Members: Waters, Crystal, Ramsden, Dale, Anderson, Carl, Kunkel, Tom, Ramsden, Dale, Sekelsky, Jeff, Vaziri, Cyrus.

Subjects/Keywords: Molecular biology; School of Medicine; Curriculum in Genetics and Molecular Biology

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Waters, C. (2015). Factors increasing the flexibility of nonhomologous end joining. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:dfed623e-e848-481b-b59b-12eaea501fa8

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Waters, Crystal. “Factors increasing the flexibility of nonhomologous end joining.” 2015. Thesis, University of North Carolina. Accessed August 05, 2020. https://cdr.lib.unc.edu/record/uuid:dfed623e-e848-481b-b59b-12eaea501fa8.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Waters, Crystal. “Factors increasing the flexibility of nonhomologous end joining.” 2015. Web. 05 Aug 2020.

Vancouver:

Waters C. Factors increasing the flexibility of nonhomologous end joining. [Internet] [Thesis]. University of North Carolina; 2015. [cited 2020 Aug 05]. Available from: https://cdr.lib.unc.edu/record/uuid:dfed623e-e848-481b-b59b-12eaea501fa8.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Waters C. Factors increasing the flexibility of nonhomologous end joining. [Thesis]. University of North Carolina; 2015. Available from: https://cdr.lib.unc.edu/record/uuid:dfed623e-e848-481b-b59b-12eaea501fa8

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.