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You searched for +publisher:"University of North Carolina" +contributor:("Trogden, Kathryn"). One record found.

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University of North Carolina

1. Trogden, Kathryn. TOG Proteins are spatially regulated by Rac-GSK3β to control interphase microtubule dynamics.

Degree: Biology, 2015, University of North Carolina

Within a cell, the ends of individual microtubules switch between three different phases: growth, shrinkage and pause without affecting the total mass of microtubule polymer. This inherent property of microtubules is termed dynamic instability. During interphase, microtubule dynamics are regulated by a class of proteins that interact primarily with the plus end of the microtubule (+TIPs). How these proteins are regulated, either through interactions with each other or through signaling pathways in the cell are poorly understood. Two +TIP protein families, the XMAP215 family, known as Mini spindles (Msps) in Drosophila and the CLASP family, known as Orbit, contain arrays of tubulin binding TOG domains. We show that in Drosophila S2 cells Orbit is phosphorylated by the kinase GSK3β, similar to what has been reported for mammalian CLASPs. At the periphery of the cell, GSK3β is inhibited by the Rho GTPase Rac and Orbit is no longer phosphorylated. Orbit is then able to interact with Msps through their C-termini, allowing Msps to bind to the lattice. Msps lattice binding is important for proper microtubule dynamics as mutations that disrupt this interaction lead to aberrant dynamics. This interaction requires the scaffolding protein Sentin to bring the two proteins into contact at the periphery of the cell. Further work has shown a role for another Rac effector kinase, PAK, in this pathway. This interaction may also be important for migration in Drosophila cells. Depletion of +TIPs leads to changes in cell movement, indicating that microtubule dynamics may play a role in motility. Advisors/Committee Members: Trogden, Kathryn, Rogers, Stephen, Bautch, Victoria, Major, Ben, Gupton, Stephanie, Slep, Kevin.

Subjects/Keywords: Cytology; College of Arts and Sciences; Department of Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Trogden, K. (2015). TOG Proteins are spatially regulated by Rac-GSK3β to control interphase microtubule dynamics. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:e7f154cb-e2de-418b-a81d-6446323d1fcb

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Trogden, Kathryn. “TOG Proteins are spatially regulated by Rac-GSK3β to control interphase microtubule dynamics.” 2015. Thesis, University of North Carolina. Accessed October 28, 2020. https://cdr.lib.unc.edu/record/uuid:e7f154cb-e2de-418b-a81d-6446323d1fcb.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Trogden, Kathryn. “TOG Proteins are spatially regulated by Rac-GSK3β to control interphase microtubule dynamics.” 2015. Web. 28 Oct 2020.

Vancouver:

Trogden K. TOG Proteins are spatially regulated by Rac-GSK3β to control interphase microtubule dynamics. [Internet] [Thesis]. University of North Carolina; 2015. [cited 2020 Oct 28]. Available from: https://cdr.lib.unc.edu/record/uuid:e7f154cb-e2de-418b-a81d-6446323d1fcb.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Trogden K. TOG Proteins are spatially regulated by Rac-GSK3β to control interphase microtubule dynamics. [Thesis]. University of North Carolina; 2015. Available from: https://cdr.lib.unc.edu/record/uuid:e7f154cb-e2de-418b-a81d-6446323d1fcb

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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