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You searched for +publisher:"University of North Carolina" +contributor:("Searles, Lillie"). Showing records 1 – 2 of 2 total matches.

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University of North Carolina

1. Mollison, Lonna. The Drosophila Melanogaster Protein Suppressor of Sable Negatively Regulates Transposon-Containing Transcripts By A Mechanism That Involves Directly Binding To Its RNA Target.

Degree: 2016, University of North Carolina

Lonna Finnic Mollison: The Drosophila melanogaster Protein Suppressor of sable [Su(s)] Negatively Regulates Transposon-Containing Transcripts by a Mechanism that Involves Directly Binding to its RNA Target (Under the direction of Lillie Searles) RNA quality control systems operate at various stages of gene expression to prevent aberrant RNAs from accumulating. The nuclear pathways that lead to the identification and elimination of defective pre-mRNAs are incompletely understood, especially in multicellular organisms. The Suppressor of sable (Su(s)) protein of D. melanogaster plays a role in this process. Su(s) is a nuclear RNA-binding protein that negatively regulates the accumulation of RNA from genes that contain transposon insertions in the 5’ transcribed region. Previous studies have shown that the Su(s)-regulatory pathway induces premature transcription termination and degradation of the resulting RNAs. Here, I present in vitro and in vivo evidence that Su(s) recognizes specific sequences in one of its biological targets. I found that a U-rich element is efficiently bound by Su(s) and a G-rich element appears to be a weaker binding site. The results of reporter gene analysis confirmed that the U-rich and G-rich elements are relevant regulatory sequences. However, a GUA-rich element that contributes significantly to this regulation is not a Su(s) binding site. These results indicate that this regulation depends on the direct binding of Su(s) and, possibly, one or more other proteins to the RNA. Advisors/Committee Members: Mollison, Lonna, Searles, Lillie, Duronio, Robert, Jones, Corbin, Marzluff, William, Peifer, Mark.

Subjects/Keywords: School of Medicine; Curriculum in Genetics and Molecular Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mollison, L. (2016). The Drosophila Melanogaster Protein Suppressor of Sable Negatively Regulates Transposon-Containing Transcripts By A Mechanism That Involves Directly Binding To Its RNA Target. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:ede5af10-16e2-4414-a012-1924567bfde9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Mollison, Lonna. “The Drosophila Melanogaster Protein Suppressor of Sable Negatively Regulates Transposon-Containing Transcripts By A Mechanism That Involves Directly Binding To Its RNA Target.” 2016. Thesis, University of North Carolina. Accessed January 20, 2021. https://cdr.lib.unc.edu/record/uuid:ede5af10-16e2-4414-a012-1924567bfde9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Mollison, Lonna. “The Drosophila Melanogaster Protein Suppressor of Sable Negatively Regulates Transposon-Containing Transcripts By A Mechanism That Involves Directly Binding To Its RNA Target.” 2016. Web. 20 Jan 2021.

Vancouver:

Mollison L. The Drosophila Melanogaster Protein Suppressor of Sable Negatively Regulates Transposon-Containing Transcripts By A Mechanism That Involves Directly Binding To Its RNA Target. [Internet] [Thesis]. University of North Carolina; 2016. [cited 2021 Jan 20]. Available from: https://cdr.lib.unc.edu/record/uuid:ede5af10-16e2-4414-a012-1924567bfde9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mollison L. The Drosophila Melanogaster Protein Suppressor of Sable Negatively Regulates Transposon-Containing Transcripts By A Mechanism That Involves Directly Binding To Its RNA Target. [Thesis]. University of North Carolina; 2016. Available from: https://cdr.lib.unc.edu/record/uuid:ede5af10-16e2-4414-a012-1924567bfde9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of North Carolina

2. Zhu, Hongbo. Improvement of the Bioavailability of Polycyclic Aromatic Hydrocarbons in Field-Contaminated Soil.

Degree: Environmental Sciences and Engineering, 2008, University of North Carolina

The contamination of soils by polycyclic aromatic hydrocarbons (PAHs) has posed a serious threat to public health and ecosystems. Bioremediation of PAH-contaminated soils is a low-cost and relatively clean strategy. However, the bioremediation is not always successful, and one of the major causes is the limited PAH bioavailability to microorganisms in the soil system. Surfactant addition is a common strategy to increase the bioavailability of PAHs in the soil. We evaluated two nonionic surfactants, one hydrophobic (Brij 30) and one hydrophilic (C12E8), for their ability to enhance the biodegradation of PAHs and their influence on groups of PAH-degrading bacteria in the bioreactor community that had previously been identified by DNA-based stable-isotope probing. The effects of each surfactant were evaluated at doses corresponding to equilibrium aqueous-phase concentrations well above the surfactant's critical micelle concentration (CMC), slightly above the CMC, and below the CMC. The concentrations of all 3- and 4-ring PAHs were significantly reduced in the soil amended with Brij 30 at the two lower doses compared to controls, whereas removal of only the 3-ring PAHs was significantly enhanced at the highest Brij 30 dose. In contrast, C12E8 did not enhance PAH removal at any dose. Brij 30 addition at the lowest dose significantly increased the desorption of most PAHs, whereas the addition of C12E8 at the lowest dose actually decreased the desorption of all PAHs. Two groups of organisms containing naphthalene- or salicylate-degrading bacteria increased in abundance substantially after incubation with both surfactants. In contrast, Brij 30 inhibited a third group of naphthalene/salicylate degraders and two groups of pyrene-degrading bacteria, and it modestly inhibited the predominant phenanthrene degrader at the highest dose. Overall, this study demonstrates that the effects of the surfactants on PAH biodegradation could be explained by their effects on PAH bioavailability, and that surfactant addition can have differential effects on populations of organisms known to be responsible for contaminant degradation within a microbial community. Advisors/Committee Members: Zhu, Hongbo, Aitken, Michael, Kamens, R. M., Miller, Cass T., Pfaender, Frederic K., Searles, Lillie.

Subjects/Keywords: Gillings School of Global Public Health; Department of Environmental Sciences and Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Zhu, H. (2008). Improvement of the Bioavailability of Polycyclic Aromatic Hydrocarbons in Field-Contaminated Soil. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:e015a97b-a101-4c27-8f4b-93d2d7aed617

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Zhu, Hongbo. “Improvement of the Bioavailability of Polycyclic Aromatic Hydrocarbons in Field-Contaminated Soil.” 2008. Thesis, University of North Carolina. Accessed January 20, 2021. https://cdr.lib.unc.edu/record/uuid:e015a97b-a101-4c27-8f4b-93d2d7aed617.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Zhu, Hongbo. “Improvement of the Bioavailability of Polycyclic Aromatic Hydrocarbons in Field-Contaminated Soil.” 2008. Web. 20 Jan 2021.

Vancouver:

Zhu H. Improvement of the Bioavailability of Polycyclic Aromatic Hydrocarbons in Field-Contaminated Soil. [Internet] [Thesis]. University of North Carolina; 2008. [cited 2021 Jan 20]. Available from: https://cdr.lib.unc.edu/record/uuid:e015a97b-a101-4c27-8f4b-93d2d7aed617.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhu H. Improvement of the Bioavailability of Polycyclic Aromatic Hydrocarbons in Field-Contaminated Soil. [Thesis]. University of North Carolina; 2008. Available from: https://cdr.lib.unc.edu/record/uuid:e015a97b-a101-4c27-8f4b-93d2d7aed617

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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