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You searched for +publisher:"University of North Carolina" +contributor:("Savoldo, Barbara"). One record found.

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University of North Carolina

1. Hong, Lee. CD30-REDIRECTED CHIMERIC ANTIGEN RECEPTOR T CELLS TARGET EMBRYONAL CARCINOMA VIA ANTIGEN-DEPENDENT AND FAS/FASL INTERACTIONS.

Degree: Microbiology and Immunology, 2018, University of North Carolina

Embryonal carcinomas (ECs) and mixed testicular germ cell tumors (TGCTs) containing EC express CD30 and are the most aggressive TGCT subtypes. Chimeric antigen receptor T cells (CAR-Ts) combine the cytotoxic properties of T cells with the antigen specificity of monoclonal antibodies to target antigen-expressing cells, such as infected or cancerous cells. CAR-Ts targeting CD30 (CD30.CAR-Ts) have shown robust anti-tumor activity against Hodgkin’s lymphoma, but have not been tested against solid tumors. We tested whether CD30.CAR-Ts could also target ECs using in vitro and in vivo models. CD30.CAR-Ts exhibited anti-tumor activity in vitro against the human EC cell lines Tera-1, Tera-2 and NCCIT, and putative EC stem cells identified by Hoechst dye staining. Cytolytic activity of CD30.CAR-Ts was complemented by sustained proliferation and pro-inflammatory cytokine production. CD30.CAR-Ts also demonstrated anti-tumor activity in an in vivo xenograft NSG mouse model of metastatic EC. Remarkably, we observed that CD30.CAR-Ts, while targeting CD30+ EC tumor cells through the CAR (i.e. antigen-dependent targeting), also eliminated surrounding CD30– EC cells in an antigen-independent manner via cell-cell contact-dependent Fas/FasL interaction. In addition, inducing Fas (CD95) expression in CD30+ but Fas– EC was sufficient to improve CD30.CAR-T anti-tumor activity. Overall, these data suggest that CD30.CAR-Ts can be used as a novel immunotherapy for ECs. Additionally, Fas/FasL interaction between tumor cells and CAR-Ts can be exploited to reduce tumor escape due to heterogeneous antigen expression or to improve CAR-T anti-tumor activity. Advisors/Committee Members: Hong, Lee, Dotti, Gianpietro, Tisch, Roland, Savoldo, Barbara, Whitmire, Jason, Wan, Yisong, Sheikh, Shehzad.

Subjects/Keywords: School of Medicine; Department of Microbiology and Immunology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hong, L. (2018). CD30-REDIRECTED CHIMERIC ANTIGEN RECEPTOR T CELLS TARGET EMBRYONAL CARCINOMA VIA ANTIGEN-DEPENDENT AND FAS/FASL INTERACTIONS. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:519f1372-1638-487e-b82e-0681308db3e0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Hong, Lee. “CD30-REDIRECTED CHIMERIC ANTIGEN RECEPTOR T CELLS TARGET EMBRYONAL CARCINOMA VIA ANTIGEN-DEPENDENT AND FAS/FASL INTERACTIONS.” 2018. Thesis, University of North Carolina. Accessed October 31, 2020. https://cdr.lib.unc.edu/record/uuid:519f1372-1638-487e-b82e-0681308db3e0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Hong, Lee. “CD30-REDIRECTED CHIMERIC ANTIGEN RECEPTOR T CELLS TARGET EMBRYONAL CARCINOMA VIA ANTIGEN-DEPENDENT AND FAS/FASL INTERACTIONS.” 2018. Web. 31 Oct 2020.

Vancouver:

Hong L. CD30-REDIRECTED CHIMERIC ANTIGEN RECEPTOR T CELLS TARGET EMBRYONAL CARCINOMA VIA ANTIGEN-DEPENDENT AND FAS/FASL INTERACTIONS. [Internet] [Thesis]. University of North Carolina; 2018. [cited 2020 Oct 31]. Available from: https://cdr.lib.unc.edu/record/uuid:519f1372-1638-487e-b82e-0681308db3e0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hong L. CD30-REDIRECTED CHIMERIC ANTIGEN RECEPTOR T CELLS TARGET EMBRYONAL CARCINOMA VIA ANTIGEN-DEPENDENT AND FAS/FASL INTERACTIONS. [Thesis]. University of North Carolina; 2018. Available from: https://cdr.lib.unc.edu/record/uuid:519f1372-1638-487e-b82e-0681308db3e0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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