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You searched for +publisher:"University of North Carolina" +contributor:("Ruf, Rebecca A. S."). One record found.

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University of North Carolina

1. Ruf, Rebecca A. S. Tyrosine and the oxidative aggregation of alpha-synuclein.

Degree: Chemistry, 2009, University of North Carolina

Oxidative stress and aggregation of the protein alpha-synuclein are thought to be key factors in Parkinson's disease. Previous work shows that cytochrome c plus H2O2 causes tyrosine-dependent in vitro peroxidative aggregation of proteins, including alpha-synuclein. Herein, I detail a method for monitoring alpha-synuclein and cytochrome c in a variety of experiments. Using this system, I examine the role of each of alpha-synuclein's four tyrosine residues and how the protein's conformation affects covalent oxidative aggregation. When alpha-synuclein adopts a collapsed conformation, tyrosine 39 is essential for wild-type-like covalent aggregation. This lone N-terminal tyrosine, however, is not required for wild type-like covalent aggregation in the presence of a denaturant or when alpha-synuclein is present in non-covalent fibrils. I also show that pre-formed oxidative aggregates are not incorporated into non-covalent fibrils. These data provide insight as to how dityrosine may be formed in Lewy bodies seen in Parkinson's disease. Additionally, I detail the progress made toward studying the toxicity of alpha-synuclein aggregates using neuronal microinjection. Advisors/Committee Members: Ruf, Rebecca A. S., Pielak, Gary J., University of North Carolina at Chapel Hill.

Subjects/Keywords: College of Arts and Sciences; Department of Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ruf, R. A. S. (2009). Tyrosine and the oxidative aggregation of alpha-synuclein. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:d6690e11-2176-477c-954b-82292a4fbec2

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ruf, Rebecca A S. “Tyrosine and the oxidative aggregation of alpha-synuclein.” 2009. Thesis, University of North Carolina. Accessed January 26, 2021. https://cdr.lib.unc.edu/record/uuid:d6690e11-2176-477c-954b-82292a4fbec2.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ruf, Rebecca A S. “Tyrosine and the oxidative aggregation of alpha-synuclein.” 2009. Web. 26 Jan 2021.

Vancouver:

Ruf RAS. Tyrosine and the oxidative aggregation of alpha-synuclein. [Internet] [Thesis]. University of North Carolina; 2009. [cited 2021 Jan 26]. Available from: https://cdr.lib.unc.edu/record/uuid:d6690e11-2176-477c-954b-82292a4fbec2.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ruf RAS. Tyrosine and the oxidative aggregation of alpha-synuclein. [Thesis]. University of North Carolina; 2009. Available from: https://cdr.lib.unc.edu/record/uuid:d6690e11-2176-477c-954b-82292a4fbec2

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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