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You searched for +publisher:"University of North Carolina" +contributor:("Rosen, Elias"). Showing records 1 – 2 of 2 total matches.

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University of North Carolina

1. Gilliland, William. Development and Applications of a Microchip Capillary Electrophoresis-High Pressure Mass Spectrometry Platform.

Degree: Chemistry, 2017, University of North Carolina

This work describes the pairing of electrospray ionization (ESI) with high pressure mass spectrometry (HPMS) with the goal of developing a miniature analytical platform as an alternative to traditional liquid chromatography-MS (LC-MS) systems. LC-MS systems are the standard for many chemical analyses and are used for a wide range of applications, but their size and complexity limits them to centralized labs. Microchip capillary electrophoresis (CE) coupled with HPMS presents an opportunity to provide an inexpensive, simple, and targeted separations-MS system. The work here demonstrates the initial steps for coupling ESI and HPMS, improvements for the analysis of small molecules, and strategies and applications for ESI-HPMS of intact proteins. The first step for ESI-HPMS was designing an interface to conduct ions from atmospheric pressure into the ion trap at ~1 Torr. The initial interface consisted of a capillary inlet and a simple DC “gate” lens. With this interface, the twenty common amino acids were detected when infused. Small peptides were detected with much greater sensitivity than amino acids for this interface, and microchip CE-HPMS of peptide standards was demonstrated. In addition, tandem mass spectrometry under HPMS conditions was performed with clusters of small molecules as well as with a small peptide (RGES). After initial development and proof-of-concept demonstration, several improvements were made with ESI-HPMS. An aperture was used in place of a capillary, and a tube lens was found to be more effective for small molecules than the “gate” lens. The RF drive frequency was increased to 30 MHz to improve resolution, and the trap size was decreased to critical dimensions of about 100 μm to maintain mass range. A SLIT trap was used to increase ion storage capacity. With these improvements, the twenty common amino acids were infused and detected with a 28-fold improvement in S/N and a 2.6-fold improvement in peak width over the previous HPMS analysis. Microchip CE-HPMS was then used for two applications: the analysis of amino acids in cell growth medium and the detection of opiates in urine. Finally, CE-HPMS was used for the analysis of intact proteins. A printed circuit board ion funnel was designed and implemented. The small proteins cytochrome c (12.3 kDa) and myoglobin (17 kDa) were detected. The mass range was adjusted to detect large proteins BSA (66 kDa) and an IgG2 (~150 kDa). CE-HPMS was then used for the detection of glycated hemoglobin in whole blood lysate. HPMS calculations of hemoglobin glycation in clinical samples were then correlated (R2 = 0.75) with HbA1c detection by immunoassay. Advisors/Committee Members: Gilliland, William, Ramsey, J. Michael, Jorgenson, James, Atkin, Joanna, Hicks, Leslie, Rosen, Elias.

Subjects/Keywords: College of Arts and Sciences; Department of Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Gilliland, W. (2017). Development and Applications of a Microchip Capillary Electrophoresis-High Pressure Mass Spectrometry Platform. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:d07d0964-86d5-42cb-a2ba-7d56d005f628

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Gilliland, William. “Development and Applications of a Microchip Capillary Electrophoresis-High Pressure Mass Spectrometry Platform.” 2017. Thesis, University of North Carolina. Accessed December 05, 2020. https://cdr.lib.unc.edu/record/uuid:d07d0964-86d5-42cb-a2ba-7d56d005f628.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Gilliland, William. “Development and Applications of a Microchip Capillary Electrophoresis-High Pressure Mass Spectrometry Platform.” 2017. Web. 05 Dec 2020.

Vancouver:

Gilliland W. Development and Applications of a Microchip Capillary Electrophoresis-High Pressure Mass Spectrometry Platform. [Internet] [Thesis]. University of North Carolina; 2017. [cited 2020 Dec 05]. Available from: https://cdr.lib.unc.edu/record/uuid:d07d0964-86d5-42cb-a2ba-7d56d005f628.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gilliland W. Development and Applications of a Microchip Capillary Electrophoresis-High Pressure Mass Spectrometry Platform. [Thesis]. University of North Carolina; 2017. Available from: https://cdr.lib.unc.edu/record/uuid:d07d0964-86d5-42cb-a2ba-7d56d005f628

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of North Carolina

2. Rosen, Elias Patrick. Morphological effects on coated aerosol kinetics.

Degree: Chemistry, 2008, University of North Carolina

Single-particle kinetic studies of the reaction between oleic acid and O3 have been conducted on two different types of core particles: polystyrene latex and silica. Oleic acid was found to adsorb to both particle types in multilayer islands that resulted in a surfactant layer of a total volume estimated to be less than one monolayer. The rate of the surface reaction between surface-adsorbed oleic acid and O3 has been shown for the first time to be influenced by the composition of the aerosol substrate in a mixed organic/inorganic particle. The reaction on the hydrophobic polystyrene core proceeded more efficiently than on a silica core at lower [O3], and the observed dependence of the observed pseudo-first order rate constant with [O3] suggests that the reaction is more efficient on the silica surface, but proceeds faster on the less polar PSL core at lower [O3] due to the increased residence time of O3 on the PSL surface. Values for the uptake coefficient, [gamma]oleic, for reaction of oleic acid on polystyrene latex spheres decrease from 2.5 x 10-5 to 1 x 10-5 with increasing [O3] from 4 to 25 ppm and overlap at high [O3] with the estimated values for [gamma]oleic on silica, which decrease from 1.6 x 10-5 to 1.3 x 10-5. Advisors/Committee Members: Rosen, Elias Patrick, Baer, Tomas.

Subjects/Keywords: College of Arts and Sciences; Department of Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Rosen, E. P. (2008). Morphological effects on coated aerosol kinetics. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:6827be6b-5311-4ac1-b201-6e677ca9d5cc

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Rosen, Elias Patrick. “Morphological effects on coated aerosol kinetics.” 2008. Thesis, University of North Carolina. Accessed December 05, 2020. https://cdr.lib.unc.edu/record/uuid:6827be6b-5311-4ac1-b201-6e677ca9d5cc.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Rosen, Elias Patrick. “Morphological effects on coated aerosol kinetics.” 2008. Web. 05 Dec 2020.

Vancouver:

Rosen EP. Morphological effects on coated aerosol kinetics. [Internet] [Thesis]. University of North Carolina; 2008. [cited 2020 Dec 05]. Available from: https://cdr.lib.unc.edu/record/uuid:6827be6b-5311-4ac1-b201-6e677ca9d5cc.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rosen EP. Morphological effects on coated aerosol kinetics. [Thesis]. University of North Carolina; 2008. Available from: https://cdr.lib.unc.edu/record/uuid:6827be6b-5311-4ac1-b201-6e677ca9d5cc

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.