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You searched for +publisher:"University of North Carolina" +contributor:("Rizzardi, Lindsay Faircloth"). One record found.

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University of North Carolina

1. Rizzardi, Lindsay Faircloth. Epigenetic Factors Are Dynamically Regulated Throughout The Cell Cycle And Are Required For Efficient DNA Replication.

Degree: 2013, University of North Carolina

Chromatin consists of DNA wrapped around a core of histone proteins that can be modified to elicit distinct cellular responses. All DNA-templated processes including DNA replication must function within the context of chromatin. The focus of this study is the interaction between the replication machinery and the chromatin structure it must traverse to faithfully replicate the genome. Individual histone modifications can promote recruitment of replication proteins directly or recruit chromatin remodelers that increase the accessibility of the underlying DNA. Our studies in budding yeast, Saccharomyces cerevisiae, have identified a specific histone modification, H3K4me2, that promotes efficient DNA replication; although, the mechanism of this regulation remains unclear. Additionally, we found that the enzyme responsible for depositing H3K4me2 is cell cycle regulated, being most abundant during G1 when cells prepare the genome for DNA replication. Another histone modification required during G1 for proper DNA replication is H4K20me1. As this modification is not found in budding yeast, our studies were undertaken in a mammalian cell culture system. The enzyme responsible for this modification had previously been identified as Set8 (a.k.a. PR-Set7) and we have begun investigating the regulation of this enzyme throughout the cell cycle and during a cellular stress response. This enzyme is degraded both during S phase and after UV-induced DNA damage by the CRL4Cdt2 E3 ubiquitin ligase complex. We discovered that this degradation after DNA damage is abrogated during mitosis and after osmotic stress. This mechanism is conserved for at least one other CRL4Cdt2 substrate, Cdt1, that is an essential replication factor. Protection from UV-induced degradation requires active Cdk1 during mitosis as inhibition of this kinase leads to degradation of both Set8 and Cdt1. We are currently investigating whether this is a direct or indirect effect. During osmotic stress in asynchronous cells, the stress MAPKs p38 and JNK may play a more important role than Cdk1. It is clear that the histone modifications and the enzymes responsible for their deposition are critical components ensuring proper DNA replication and as such are tightly regulated. Future work will elucidate the exact mechanisms by which these chromatin factors influence DNA replication. Advisors/Committee Members: Rizzardi, Lindsay Faircloth, Cook, Jean, Duronio, Robert, Strahl, Brian, Errede, Beverly, Baldwin, Albert.

Subjects/Keywords: School of Medicine; Curriculum in Genetics and Molecular Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Rizzardi, L. F. (2013). Epigenetic Factors Are Dynamically Regulated Throughout The Cell Cycle And Are Required For Efficient DNA Replication. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:08380d60-c1fb-48fe-aa17-ba65b9fffae9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Rizzardi, Lindsay Faircloth. “Epigenetic Factors Are Dynamically Regulated Throughout The Cell Cycle And Are Required For Efficient DNA Replication.” 2013. Thesis, University of North Carolina. Accessed October 01, 2020. https://cdr.lib.unc.edu/record/uuid:08380d60-c1fb-48fe-aa17-ba65b9fffae9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Rizzardi, Lindsay Faircloth. “Epigenetic Factors Are Dynamically Regulated Throughout The Cell Cycle And Are Required For Efficient DNA Replication.” 2013. Web. 01 Oct 2020.

Vancouver:

Rizzardi LF. Epigenetic Factors Are Dynamically Regulated Throughout The Cell Cycle And Are Required For Efficient DNA Replication. [Internet] [Thesis]. University of North Carolina; 2013. [cited 2020 Oct 01]. Available from: https://cdr.lib.unc.edu/record/uuid:08380d60-c1fb-48fe-aa17-ba65b9fffae9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rizzardi LF. Epigenetic Factors Are Dynamically Regulated Throughout The Cell Cycle And Are Required For Efficient DNA Replication. [Thesis]. University of North Carolina; 2013. Available from: https://cdr.lib.unc.edu/record/uuid:08380d60-c1fb-48fe-aa17-ba65b9fffae9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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