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University of North Carolina

1. Pham, Yen Bao. Characterization of a Tryptophanyl-tRNA Synthetase Urzyme and its Intramolecular Complementation Establishes a Model to Study the Catalytic Mechanism and Evolution of the Contemporary Class I Aminoacyl-tRNA Synthetases.

Degree: Biochemistry and Biophysics, 2010, University of North Carolina

Aminoacyl-tRNA synthetases (AaRSs) comprise a crucial group of enzymes catalyzing a two-step reaction to activate amino acids and acylate their cognate tRNAs. It is widely known that highly conserved sequences and motifs divide the synthetases evenly into two distinct classes of ten members each. However, the questions of how they might have emerged and evolved and what a reasonable candidate for the ancestral enzyme would be like still remain unsolved. Answering these would not only shed light on the aaRSs evolution but could also help unravel the critical point when the extremely high kinetic barrier of amino acid activation was bypassed, allowing the synthesis of primitive peptide chains. The research object of the studies is a tryptophanyl-tRNA synthetase (TrpRS) Urzyme (Ur-: prefix means primitive, original) which would fit the description of a potential candidate for the ancestral class I aaRS. The TrpRS Urzyme is designed to retain class I signature sequences that comprise the ATP-binding Rossmann fold. Interestingly, it has similar affinity for ATP compared to the full length wild type enzyme but surprisingly low affinity for the cognate amino acid substrate, tryptophan. This might be explained by absence of the connecting peptide 1 (CP1), an insertion that wraps around the tryptophan binding site in the contemporary enzyme and occurs at the same location as the editing domain in four other class I aaRSs (MetRS, LeuRS, IleRS, ValRS). An in cis modular complementation experiment might point out a potential role for CP1 in configuring a confirmation that has high affinity for tryptophan, functionally similar to a primitive proofreading function. Furthermore, an Urzyme containing the C-terminal domain (CTD) in cis has been constructed to further explore CTD's role in tryptophan activation and tRNATrp acylation. The active TrpRS Urzyme establishment confirms an essential prediction of, and is therefore, the first experiment to provide evidential support for the unique Rodin-Ohno hypothesis about the earlier existence of a sense-antisense coding system for both aaRS classes. The successful design of the TrpRS Urzyme has also provided a precedent that has led to the construction of class I and class II aaRS Urzymes of comparable lengths. Advisors/Committee Members: Pham, Yen Bao, Carter, Charles.

Subjects/Keywords: School of Medicine; Department of Biochemistry and Biophysics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Pham, Y. B. (2010). Characterization of a Tryptophanyl-tRNA Synthetase Urzyme and its Intramolecular Complementation Establishes a Model to Study the Catalytic Mechanism and Evolution of the Contemporary Class I Aminoacyl-tRNA Synthetases. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:8953fcbd-d082-4011-a700-6f46cf19201f

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Pham, Yen Bao. “Characterization of a Tryptophanyl-tRNA Synthetase Urzyme and its Intramolecular Complementation Establishes a Model to Study the Catalytic Mechanism and Evolution of the Contemporary Class I Aminoacyl-tRNA Synthetases.” 2010. Thesis, University of North Carolina. Accessed January 18, 2021. https://cdr.lib.unc.edu/record/uuid:8953fcbd-d082-4011-a700-6f46cf19201f.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Pham, Yen Bao. “Characterization of a Tryptophanyl-tRNA Synthetase Urzyme and its Intramolecular Complementation Establishes a Model to Study the Catalytic Mechanism and Evolution of the Contemporary Class I Aminoacyl-tRNA Synthetases.” 2010. Web. 18 Jan 2021.

Vancouver:

Pham YB. Characterization of a Tryptophanyl-tRNA Synthetase Urzyme and its Intramolecular Complementation Establishes a Model to Study the Catalytic Mechanism and Evolution of the Contemporary Class I Aminoacyl-tRNA Synthetases. [Internet] [Thesis]. University of North Carolina; 2010. [cited 2021 Jan 18]. Available from: https://cdr.lib.unc.edu/record/uuid:8953fcbd-d082-4011-a700-6f46cf19201f.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pham YB. Characterization of a Tryptophanyl-tRNA Synthetase Urzyme and its Intramolecular Complementation Establishes a Model to Study the Catalytic Mechanism and Evolution of the Contemporary Class I Aminoacyl-tRNA Synthetases. [Thesis]. University of North Carolina; 2010. Available from: https://cdr.lib.unc.edu/record/uuid:8953fcbd-d082-4011-a700-6f46cf19201f

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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