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You searched for +publisher:"University of North Carolina" +contributor:("Leopold, Luciana"). Showing records 1 – 2 of 2 total matches.

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University of North Carolina

1. Leopold, Luciana Eleanor. Development of Real-Time PCR Assays for the Quantitative Detection of Herpesviruses.

Degree: 2008, University of North Carolina

Kaposis's sarcoma-associated Herpesvirus (KSHV) and Human Epstein-Barr virus (EBV) are gamma herpesviruses that can cause serious complications in immunocompromised patients. Quantitative detection is critical not only as a diagnostic tool in patients, but also as a method for basic herpesvirology research. Here the development and validation of four real-time PCR assays to quantify viral load of KSHV and EBV in samples is described. The assays are: individual viral load assays for KSHV and EBV, and high-throughput viral load screens for KSHV and EBV. The assays were developed for validation of animal models, drug response studies, basic virology research and medical diagnostics. The data indicate that these are accurate, consistent, and cost-effective assays for the detection of KSHV and EBV infection in various kinds of samples. Advisors/Committee Members: Leopold, Luciana Eleanor, Dittmer, Dirk.

Subjects/Keywords: School of Medicine; Curriculum in Genetics and Molecular Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Leopold, L. E. (2008). Development of Real-Time PCR Assays for the Quantitative Detection of Herpesviruses. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:d2f37589-babe-418b-9d5d-c5bcf30933df

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Leopold, Luciana Eleanor. “Development of Real-Time PCR Assays for the Quantitative Detection of Herpesviruses.” 2008. Thesis, University of North Carolina. Accessed September 21, 2020. https://cdr.lib.unc.edu/record/uuid:d2f37589-babe-418b-9d5d-c5bcf30933df.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Leopold, Luciana Eleanor. “Development of Real-Time PCR Assays for the Quantitative Detection of Herpesviruses.” 2008. Web. 21 Sep 2020.

Vancouver:

Leopold LE. Development of Real-Time PCR Assays for the Quantitative Detection of Herpesviruses. [Internet] [Thesis]. University of North Carolina; 2008. [cited 2020 Sep 21]. Available from: https://cdr.lib.unc.edu/record/uuid:d2f37589-babe-418b-9d5d-c5bcf30933df.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leopold LE. Development of Real-Time PCR Assays for the Quantitative Detection of Herpesviruses. [Thesis]. University of North Carolina; 2008. Available from: https://cdr.lib.unc.edu/record/uuid:d2f37589-babe-418b-9d5d-c5bcf30933df

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of North Carolina

2. Leopold, Luciana. SURVEILLANCE AND TARGETING OF ABERRANT TRANSCRIPTS IN CAENORHABDITIS ELEGANS.

Degree: 2014, University of North Carolina

An organism requires mechanisms with which to protect the fidelity and integrity of the genome, transcriptome, and proteome in order not only to remain healthy and alive, but also to pass on viable genetic material to its offspring. To this end, the cell is constantly surveying the transcripts being expressed. When aberrant transcripts are produced, whether they be from endogenous or exogenous sources, mechanisms are required in order to silence or eradicate such messages before they become toxic to the cell. Two such mechanisms are nonsense-mediated mRNA decay (NMD), which targets transcripts bearing a premature termination codon (PTC), and RNA-induced epigenetic silencing (RNAe), which silences foreign loci in the genome in a heritable, stable manner. We have identified targets that require unique kinds of silencing that utilize already established NMD or RNAe machinery, but represent deviations from the standard. For example, the unc-54(r293) transcript was previously thought to be silenced by standard NMD, however I have shown that unc-54(r293) does not contain the canonical target PTC, and instead has a more complex molecular target. I also show that the integral SMG-1 component of the NMD machinery interacts with this target in a manner that is distinct from that of traditional NMD, as illustrated by the novel smg-1(yp3) separation-of-function allele. In the next example, we show that permanent, stable transgene silencing like that generated by RNAe in a single generation, can be attained in certain transgenes only after several generations of successive crossing. These processes are molecularly similar, as they both require the Argonaute protein PRG-1. However, they differ in that single-generation RNAe silencing becomes independent of PRG-1 after initiation, whereas the silencing we observe in multigenerational RNAe requires PRG-1 in order to maintain its silent state. Advisors/Committee Members: Leopold, Luciana, Ahmed, Shawn, Marzluff, William, Conlon, Frank, Bautch, Victoria, Reiner, David.

Subjects/Keywords: Genetics; Molecular biology; School of Medicine; Curriculum in Genetics and Molecular Biology

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Leopold, L. (2014). SURVEILLANCE AND TARGETING OF ABERRANT TRANSCRIPTS IN CAENORHABDITIS ELEGANS. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:ada89d4f-4df5-4185-af49-4684fa99b655

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Leopold, Luciana. “SURVEILLANCE AND TARGETING OF ABERRANT TRANSCRIPTS IN CAENORHABDITIS ELEGANS.” 2014. Thesis, University of North Carolina. Accessed September 21, 2020. https://cdr.lib.unc.edu/record/uuid:ada89d4f-4df5-4185-af49-4684fa99b655.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Leopold, Luciana. “SURVEILLANCE AND TARGETING OF ABERRANT TRANSCRIPTS IN CAENORHABDITIS ELEGANS.” 2014. Web. 21 Sep 2020.

Vancouver:

Leopold L. SURVEILLANCE AND TARGETING OF ABERRANT TRANSCRIPTS IN CAENORHABDITIS ELEGANS. [Internet] [Thesis]. University of North Carolina; 2014. [cited 2020 Sep 21]. Available from: https://cdr.lib.unc.edu/record/uuid:ada89d4f-4df5-4185-af49-4684fa99b655.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leopold L. SURVEILLANCE AND TARGETING OF ABERRANT TRANSCRIPTS IN CAENORHABDITIS ELEGANS. [Thesis]. University of North Carolina; 2014. Available from: https://cdr.lib.unc.edu/record/uuid:ada89d4f-4df5-4185-af49-4684fa99b655

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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