Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for +publisher:"University of North Carolina" +contributor:("Geddings, Julia"). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of North Carolina

1. Geddings, Julia. The Role of Tumor Microvesicles in Cancer-Associated Thrombosis.

Degree: Pathology and Laboratory Medicine, 2015, University of North Carolina

Cancer patients have a ~4 -fold increased risk of venous thromboembolism (VTE) compared with the general population, and cancer patients with VTE have reduced survival compared with non-VTE patients. Microvesicles (MVs), also known as microparticles (MPs), are small membrane vesicles that are constitutively released from tumors, and tumor cell-derived, tissue factor (TF)-positive (TF+) MVs may contribute to thrombosis in cancer patients. I tested the hypothesis that tumor cell-derived, TF+ TMVs activate platelets in vitro and in vivo, and this contributes to cancer-associated VTE. For this study I first had to establish a reproducible model of cancer-associated thrombosis. The in vivo model chosen for this purpose was the inferior vena cava (IVC) stenosis model in mice. This model was chosen because it mimics certain features of human deep vein thrombosis, including the triggering of thrombosis with minimal endothelial damage, maintenance of blood flow at the site of thrombosis, and chronic thrombus development that can be followed over time in vivo. The strengths and weaknesses of this model are summarized in chapter 2 of this dissertation. The IVC stenosis model of thrombosis was used to evaluate the role of TF+ TMVs in the enhancement of thrombosis. In the study included as chapter 3 in this dissertation, I found that TF+ TMVs isolated from two human pancreatic cell carcinoma cells lines activate isolated human platelets in a TF- and thrombin-dependent manner in vitro. TF+ TMV-mediated platelet activation was also inhibited by the ADP scavenger apyrase and the P2Y12 receptor inhibitor 2MesAMP. Further, TF+ TMVs enhanced thrombosis in two mouse models of venous thrombosis in a TF-dependent manner. TF+ TMV-enhanced thrombosis was reduced in Par4 deficient mice and in wild-type mice treated with the platelet inhibitor clopidogrel, suggesting that TF+ TMV-enhanced thrombosis in mice was, in part, dependent on platelets. These studies identify TF+ TMV-induced platelet activation as a possible mechanism of thrombosis in cancer and suggest that platelet inhibitors, such as aspirin and clopidogrel, may be considered for the prevention of VTE in high risk cancer patients. Advisors/Committee Members: Geddings, Julia, Mackman, Nigel, Homeister, Jonathon, Kasthuri, Raj, Key, Nigel, Weissman, Bernard, Bergmeier, Wolfgang.

Subjects/Keywords: Medicine; Biology; Pathology; School of Medicine; Department of Pathology and Laboratory Medicine

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Geddings, J. (2015). The Role of Tumor Microvesicles in Cancer-Associated Thrombosis. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:3d55e13e-65a0-47b3-885e-91787f00f01f

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Geddings, Julia. “The Role of Tumor Microvesicles in Cancer-Associated Thrombosis.” 2015. Thesis, University of North Carolina. Accessed November 26, 2020. https://cdr.lib.unc.edu/record/uuid:3d55e13e-65a0-47b3-885e-91787f00f01f.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Geddings, Julia. “The Role of Tumor Microvesicles in Cancer-Associated Thrombosis.” 2015. Web. 26 Nov 2020.

Vancouver:

Geddings J. The Role of Tumor Microvesicles in Cancer-Associated Thrombosis. [Internet] [Thesis]. University of North Carolina; 2015. [cited 2020 Nov 26]. Available from: https://cdr.lib.unc.edu/record/uuid:3d55e13e-65a0-47b3-885e-91787f00f01f.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Geddings J. The Role of Tumor Microvesicles in Cancer-Associated Thrombosis. [Thesis]. University of North Carolina; 2015. Available from: https://cdr.lib.unc.edu/record/uuid:3d55e13e-65a0-47b3-885e-91787f00f01f

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.