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You searched for +publisher:"University of North Carolina" +contributor:("Garrett, Christopher"). One record found.

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University of North Carolina

1. Garrett, Christopher. Characterization of a Key Mycobacterium tuberculosis Lipase, LipY.

Degree: Biochemistry and Biophysics, 2015, University of North Carolina

The causative agent of tuberculosis, Mycobacterium tuberculosis is estimated to be present in roughly a third of the world’s population. One of the hallmarks of the pathogen is its reliance on lipids as a source of energy in order to survive in a non-replicating state and ultimately cause infection. A key player in lipid utilization by M. tuberculosis is the triglyceride lipase, LipY. LipY is the rate-limiting catalyst in the mobilization of free fatty acids from triglycerides acting on lipid bodies both within the tubercule bacillus and outside the bacterium. LipY also belongs to the PE/PPE family of proteins, a group of approximately 169 virulence-associated proteins characterized by a proline-glutamate or proline-proline-glutamate motif at their N-termini. It has been suggested that the PE domain of LipY may act as a regulator of enzymatic activity. Using several biochemical and activity-based approaches, we established a method for purifying LipY with improved resolution. We also introduced a rapid and reliable method for quantification of active sites for serine hydrolases using activity-based protein profiling (ABPP). Finally, by imploring these methodologies, we discovered that the PE domain of LipY acts as a non-competitive inhibitor of LipY enzymatic activity when it is attached to the mature lipase. The methodologies and evidence presented here may lead to discovery of additional PE/PPE family enzymes with roles in M. tuberculosis lipid metabolism and/or virulence. This work may also lead to expansion of our understanding of the roles of PE/PPE proteins and domains. Advisors/Committee Members: Garrett, Christopher, Neher, Saskia, Carter, Charles, Redinbo, Matthew R., Braunstein, Miriam, Holly, Stephen.

Subjects/Keywords: Biochemistry; Biophysics; School of Medicine; Department of Biochemistry and Biophysics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Garrett, C. (2015). Characterization of a Key Mycobacterium tuberculosis Lipase, LipY. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:80107563-6511-4298-9a76-7185a0292ea6

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Garrett, Christopher. “Characterization of a Key Mycobacterium tuberculosis Lipase, LipY.” 2015. Thesis, University of North Carolina. Accessed January 18, 2021. https://cdr.lib.unc.edu/record/uuid:80107563-6511-4298-9a76-7185a0292ea6.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Garrett, Christopher. “Characterization of a Key Mycobacterium tuberculosis Lipase, LipY.” 2015. Web. 18 Jan 2021.

Vancouver:

Garrett C. Characterization of a Key Mycobacterium tuberculosis Lipase, LipY. [Internet] [Thesis]. University of North Carolina; 2015. [cited 2021 Jan 18]. Available from: https://cdr.lib.unc.edu/record/uuid:80107563-6511-4298-9a76-7185a0292ea6.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Garrett C. Characterization of a Key Mycobacterium tuberculosis Lipase, LipY. [Thesis]. University of North Carolina; 2015. Available from: https://cdr.lib.unc.edu/record/uuid:80107563-6511-4298-9a76-7185a0292ea6

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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