Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for +publisher:"University of North Carolina" +contributor:("Friedman, Adam"). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of North Carolina

1. Friedman, Adam. THE SELECTION OF HIGHLY STABLE APTAMERS FROM A 2’-FULLY MODIFIED fGmH RNA LIBRARY.

Degree: 2015, University of North Carolina

When developed as tools for applications within biological systems, RNA aptamers immediately face numerous obstacles, in particular nuclease degradation and post-selection 2’ modification. This study aimed to develop a novel class of highly stable, 2’-fully modified RNA aptamers directly selectable from a fGmH RNA library with improved nuclease stability. The facile transcription of a fGmH (2’-F-dG, 2’-OMe-dA/dC/dU) RNA library was performed, from which aptamers were directly selected that bind Staphylococcus aureus Protein A (SpA). The superior nuclease and serum stability of these fGmH aptamers was demonstrated in comparison to 2’-partially modified RNA variants. Characterizations of fGmH RNA aptamers binding to purified SpA and to endogenous SpA present on the surface of S. aureus cells demonstrated fGmH RNA aptamer selectivity and stability. Significantly, fGmH RNA aptamers were able to functionalize, stabilize, and specifically deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus with SpA-dependent antimicrobial effects. Attempted selection of fGmH RNA aptamers against other targets, namely the PD-1 immune checkpoint, led to the selection of a single non-binding sequence during the selection against PD-1, as well as several non-binding sequence motif groups during the selection against PD-L1. Overall, this dissertation demonstrates the selection and deployment of Protein A binding fGmH RNA aptamers against S. aureus, discusses hypotheses and results aimed at explaining the selection of PD-1 and PD-L1 non-binding sequences, and describes a novel aptamer class with considerable potential to improve the in vivo applicability of nucleic acid-based affinity molecules. Advisors/Committee Members: Friedman, Adam, Liu, Rihe, Jarstfer, Michael, Lee, Andrew, Salama, April, Wang, Zefeng.

Subjects/Keywords: Pharmaceutical chemistry; Biochemistry; Molecular biology; Eshelman School of Pharmacy; Division of Chemical Biology and Medicinal Chemistry

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Friedman, A. (2015). THE SELECTION OF HIGHLY STABLE APTAMERS FROM A 2’-FULLY MODIFIED fGmH RNA LIBRARY. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:c82dc258-3481-4204-a6a3-0f06792bacd7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Friedman, Adam. “THE SELECTION OF HIGHLY STABLE APTAMERS FROM A 2’-FULLY MODIFIED fGmH RNA LIBRARY.” 2015. Thesis, University of North Carolina. Accessed January 21, 2021. https://cdr.lib.unc.edu/record/uuid:c82dc258-3481-4204-a6a3-0f06792bacd7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Friedman, Adam. “THE SELECTION OF HIGHLY STABLE APTAMERS FROM A 2’-FULLY MODIFIED fGmH RNA LIBRARY.” 2015. Web. 21 Jan 2021.

Vancouver:

Friedman A. THE SELECTION OF HIGHLY STABLE APTAMERS FROM A 2’-FULLY MODIFIED fGmH RNA LIBRARY. [Internet] [Thesis]. University of North Carolina; 2015. [cited 2021 Jan 21]. Available from: https://cdr.lib.unc.edu/record/uuid:c82dc258-3481-4204-a6a3-0f06792bacd7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Friedman A. THE SELECTION OF HIGHLY STABLE APTAMERS FROM A 2’-FULLY MODIFIED fGmH RNA LIBRARY. [Thesis]. University of North Carolina; 2015. Available from: https://cdr.lib.unc.edu/record/uuid:c82dc258-3481-4204-a6a3-0f06792bacd7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.