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You searched for +publisher:"University of North Carolina" +contributor:("Charlton, Lisa M."). One record found.

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University of North Carolina

1. Charlton, Lisa M. Protein behavior in crowded environments.

Degree: Chemistry, 2008, University of North Carolina

The cell’s interior is a complex milieu where proteins exist in an environment crowded with other macromolecules. The influence of macromolecular crowding is prevalent in every cellular function—from metabolism to signal transduction to protein folding. It is even hypothesized that macromolecular crowding dictates the organization of the intracellular environment and the evolution of a single-cell species. Even though proteins are prevalent in every biochemical process, we lack fundamental knowledge about how crowding affects proteins. When studying proteins in a crowded environment, one can either develop a system that mimics the cellular interior or develop a technique that observes proteins inside cells. Herein, I report a residue-level interrogation on the stability of chymotrypsin inhibitor 2 under macromolecular crowded conditions. This is the first study that offers comprehensive information about the effects of crowding on the stability of the native state of a globular protein. I also present observations about the behavior of globular, partially-folded, and natively-disordered proteins inside living E. coli and the consequences for in-cell NMR. Lastly, I discuss our progress in transitioning from in-cell NMR for living E. coli to the yeast, Pichia pastoris. Advisors/Committee Members: Charlton, Lisa M., Pielak, Gary J., University of North Carolina at Chapel Hill.

Subjects/Keywords: College of Arts and Sciences; Department of Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Charlton, L. M. (2008). Protein behavior in crowded environments. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:f717815f-a4bb-4f1d-9ff9-432e81e9032a

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Charlton, Lisa M. “Protein behavior in crowded environments.” 2008. Thesis, University of North Carolina. Accessed January 19, 2021. https://cdr.lib.unc.edu/record/uuid:f717815f-a4bb-4f1d-9ff9-432e81e9032a.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Charlton, Lisa M. “Protein behavior in crowded environments.” 2008. Web. 19 Jan 2021.

Vancouver:

Charlton LM. Protein behavior in crowded environments. [Internet] [Thesis]. University of North Carolina; 2008. [cited 2021 Jan 19]. Available from: https://cdr.lib.unc.edu/record/uuid:f717815f-a4bb-4f1d-9ff9-432e81e9032a.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Charlton LM. Protein behavior in crowded environments. [Thesis]. University of North Carolina; 2008. Available from: https://cdr.lib.unc.edu/record/uuid:f717815f-a4bb-4f1d-9ff9-432e81e9032a

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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