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You searched for +publisher:"University of North Carolina" +contributor:("Boyer, Joshua Alan"). One record found.

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University of North Carolina

1. Boyer, Joshua Alan. Dialing-Up Distal Sites in Proteins: The Search for the Mechanical Consequences of Thermodynamic Coupling in eglin C.

Degree: Biochemistry and Biophysics, 2009, University of North Carolina

Proteins have evolved to exploit long-range dynamic and structural effects as a means of regulating function. Understanding this communication between distal sites in proteins is therefore vital to our comprehension of such phenomena as allostery. Structural effects have been suggested but are insufficient for conclusively describing distal-site communication. We believe that understanding protein dynamics may be imperative to comprehending these remote effects. The intent of this dissertation and work herein, is to elucidate the bases underlying thermodynamic coupling/non-additivity, one such long-range effect. The absence of ligands or products in protein folding studies provides a simple background for examining this most basic communication, non-additivity. However, traditional coupling-measuring methods may be too insensitive to report on the idiosyncratic behavior linking distal sites. To increase sensitivity, we have developed hydrogen exchange (HX) techniques that employ NMR's atomic resolution to standard methodologies of double mutant cycles. Due to the large number of probes and their ability to report on equilibria that do not require global unfolding transitions, this method is more likely to perceive underlying dynamic bases than its traditional counterparts. Similarly, to increase the scope and sensitivity of dynamic probes, we applied aromatic 13C relaxation experiments. Together, these methods have identified the co-localization of dynamic and thermodynamic responses in eglin c, further insinuating a relationship between motion and non-additivity. Advisors/Committee Members: Boyer, Joshua Alan, Lee, Andrew.

Subjects/Keywords: School of Medicine; Department of Biochemistry and Biophysics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Boyer, J. A. (2009). Dialing-Up Distal Sites in Proteins: The Search for the Mechanical Consequences of Thermodynamic Coupling in eglin C. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:961e7408-a3c0-461b-a588-1c65039f49d4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Boyer, Joshua Alan. “Dialing-Up Distal Sites in Proteins: The Search for the Mechanical Consequences of Thermodynamic Coupling in eglin C.” 2009. Thesis, University of North Carolina. Accessed January 26, 2021. https://cdr.lib.unc.edu/record/uuid:961e7408-a3c0-461b-a588-1c65039f49d4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Boyer, Joshua Alan. “Dialing-Up Distal Sites in Proteins: The Search for the Mechanical Consequences of Thermodynamic Coupling in eglin C.” 2009. Web. 26 Jan 2021.

Vancouver:

Boyer JA. Dialing-Up Distal Sites in Proteins: The Search for the Mechanical Consequences of Thermodynamic Coupling in eglin C. [Internet] [Thesis]. University of North Carolina; 2009. [cited 2021 Jan 26]. Available from: https://cdr.lib.unc.edu/record/uuid:961e7408-a3c0-461b-a588-1c65039f49d4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boyer JA. Dialing-Up Distal Sites in Proteins: The Search for the Mechanical Consequences of Thermodynamic Coupling in eglin C. [Thesis]. University of North Carolina; 2009. Available from: https://cdr.lib.unc.edu/record/uuid:961e7408-a3c0-461b-a588-1c65039f49d4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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