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You searched for +publisher:"University of North Carolina" +contributor:("Beicker, Kellie"). One record found.

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University of North Carolina

1. Beicker, Kellie. Development of a Combined Atomic Force Microscopy and Side-View Imaging System for Mechanotransduction Research.

Degree: Physics and Astronomy, 2016, University of North Carolina

Key metrics for understanding cell response to mechanical stimuli include rearrangement of the cytoskeletal and nucleoskeletal structure, induced strains and biochemical distributions; however, structural information during applied stress is limited by our ability to image cells under load. In order to study the mechanics of single cells and subcellular components under load, I have developed a unique imaging system that combines an atomic force microscope (AFM) with both vertical light-sheet (VLS) illumination and a new imaging technique called PRISM – Pathway Rotated Imaging for Sideways Microscopy. The combined AFM and PRISM system facilitates the imaging of cell deformation in the direction of applied force with accompanying pico-Newton resolution force measurements. The additional inclusion of light-sheet microscopy improves the signal-to-noise ratio achieved by illumination of only a thin layer of the cell. This system is capable of pico-newton resolution force measurements with simultaneous side-view high frame rate imaging for single-molecule and single-cell force studies. Longer-term goals for this instrument are to investigate how external mechanical stimuli, specifically single-molecule interactions, alter gene expression, motility, and differentiation. The overall goal of my dissertation work is to design a tool useful for mechanobiology studies of single cells. This requires the design and implementation of PRISM and VLS systems that can be coupled to the standard Asylum AFM on inverted optical microscope. I also examine the strategy and implementation of experimental procedures and data analysis pipelines for single-cell and single-molecule force spectroscopy. These goals can be broken down as follows: • Performed single-molecule force spectroscopy experiments. • Performed single-cell force spectroscopy experiments. • Constructed and characterized the side-view microscopy system. • Applied combined AFM and side-vew microscopy system. Advisors/Committee Members: Beicker, Kellie, Superfine, Richard, Falvo, Michael, Cheney, Richard, Mitran, Sorin, Cecil, Gerald.

Subjects/Keywords: College of Arts and Sciences; Department of Physics and Astronomy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Beicker, K. (2016). Development of a Combined Atomic Force Microscopy and Side-View Imaging System for Mechanotransduction Research. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:30c8dfa8-7037-4dce-8023-aaacda3feb72

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Beicker, Kellie. “Development of a Combined Atomic Force Microscopy and Side-View Imaging System for Mechanotransduction Research.” 2016. Thesis, University of North Carolina. Accessed January 23, 2021. https://cdr.lib.unc.edu/record/uuid:30c8dfa8-7037-4dce-8023-aaacda3feb72.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Beicker, Kellie. “Development of a Combined Atomic Force Microscopy and Side-View Imaging System for Mechanotransduction Research.” 2016. Web. 23 Jan 2021.

Vancouver:

Beicker K. Development of a Combined Atomic Force Microscopy and Side-View Imaging System for Mechanotransduction Research. [Internet] [Thesis]. University of North Carolina; 2016. [cited 2021 Jan 23]. Available from: https://cdr.lib.unc.edu/record/uuid:30c8dfa8-7037-4dce-8023-aaacda3feb72.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Beicker K. Development of a Combined Atomic Force Microscopy and Side-View Imaging System for Mechanotransduction Research. [Thesis]. University of North Carolina; 2016. Available from: https://cdr.lib.unc.edu/record/uuid:30c8dfa8-7037-4dce-8023-aaacda3feb72

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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