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You searched for +publisher:"University of North Carolina" +contributor:("Bartsch, Victoria"). One record found.

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University of North Carolina

1. Bartsch, Victoria. CHARACTERIZATION OF DIACYLGLYCEROL KINASES ETA AND IOTA IN ITCH, PAIN, AND PSYCHOPATHOLOGICAL BEHAVIOR IN MICE.

Degree: Cell Biology and Physiology, 2018, University of North Carolina

Diacylglycerol kinases (DGK) control diverse signaling functions. Expression of the iota and eta isoforms of DGK (Dgki and Dgkh, respectively) are enriched in mouse neuronal tissues. By overexpressing Dgki and Dgkh in HEK293 cells, we found that Dgki and Dgkh enhanced G-protein-coupled receptor signaling and phosphorylated diacylglycerol and monoacylglycerol. We acquired a Dgki¬-knockout mouse and generated a Dgkh-knockout mouse and a double Dgki/Dgkh knockout (dKO) mouse. Using the Dgki-/-, Dgkh-/-, and dKO mice, we elucidated the role of Dgki and Dgkh in regulating behaviors associated with the neuronal tissues in which they are expressed. Dgki and Dgkh are highly expressed in small-diameter dorsal root ganglia (DRG) neurons, which detect pruritogenic (itch-causing) and algogenic (pain-causing) stimuli. We found that in vivo sensitivity to histamine—but not other pruritogens—was enhanced in male and female Dgki-/- mice, but was attenuated in Dgkh-/- males. Interestingly, dKO mice phenocopied the histamine sensitivity of the Dgki-/- mice. In contrast, baseline pain sensitivity and pain sensitization post-injury were equivalent between wild type (WT) and Dgki-/-, Dgkh-/-, or dKO mice. Even though diacylglycerol and monoacylglycerol kinase activity was reduced in DRGs from Dgkh-/- and dKO mice, in vitro signaling induced by pruritogens and algogens in DRG neurons was unaffected by Dgki or Dgkh deletion. Genome-wide association studies linked DGKH and DGKI to mood disorders, and both genes are expressed throughout the brain. dKO mice—but not Dgki-/- or Dgkh-/- mice—showed behavioral signs of anxiety and mania. Dgki-/- and dKO males showed hyperactivity and hyperexploratory behavior, as well. In addition to the psychopathological phenotypes, dKO females demonstrated deficits in maternal care. Fewer than 30% of newborn pups raised by dKO females survived to weaning; however, 85% of pups born from dKO dams survived when fostered by WT dams. Pups raised by dKO dams had smaller milk spots and reduced weight, indicative of impaired nursing. Together, our research suggests that Dgki and Dgkh regulate behavioral responses to histamine, without affecting responses to other pruritogens or algogens, and that combined deletion of Dgkh and Dgki disrupts mood-disorder-related phenotypes and impairs maternal behavior in the early postpartum period. Advisors/Committee Members: Bartsch, Victoria, Zylka, Mark, Otey, Carol, Nackley, Andrea, Nicholas, Robert, Tarran, Robert.

Subjects/Keywords: School of Medicine; Department of Cell Biology and Physiology

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APA (6th Edition):

Bartsch, V. (2018). CHARACTERIZATION OF DIACYLGLYCEROL KINASES ETA AND IOTA IN ITCH, PAIN, AND PSYCHOPATHOLOGICAL BEHAVIOR IN MICE. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:6619d1ae-66a1-4efd-8213-5e01e00c418f

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Bartsch, Victoria. “CHARACTERIZATION OF DIACYLGLYCEROL KINASES ETA AND IOTA IN ITCH, PAIN, AND PSYCHOPATHOLOGICAL BEHAVIOR IN MICE.” 2018. Thesis, University of North Carolina. Accessed September 19, 2020. https://cdr.lib.unc.edu/record/uuid:6619d1ae-66a1-4efd-8213-5e01e00c418f.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Bartsch, Victoria. “CHARACTERIZATION OF DIACYLGLYCEROL KINASES ETA AND IOTA IN ITCH, PAIN, AND PSYCHOPATHOLOGICAL BEHAVIOR IN MICE.” 2018. Web. 19 Sep 2020.

Vancouver:

Bartsch V. CHARACTERIZATION OF DIACYLGLYCEROL KINASES ETA AND IOTA IN ITCH, PAIN, AND PSYCHOPATHOLOGICAL BEHAVIOR IN MICE. [Internet] [Thesis]. University of North Carolina; 2018. [cited 2020 Sep 19]. Available from: https://cdr.lib.unc.edu/record/uuid:6619d1ae-66a1-4efd-8213-5e01e00c418f.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bartsch V. CHARACTERIZATION OF DIACYLGLYCEROL KINASES ETA AND IOTA IN ITCH, PAIN, AND PSYCHOPATHOLOGICAL BEHAVIOR IN MICE. [Thesis]. University of North Carolina; 2018. Available from: https://cdr.lib.unc.edu/record/uuid:6619d1ae-66a1-4efd-8213-5e01e00c418f

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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