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University of North Carolina

1. Barnes, Christopher O. Protein in-cell NMR in Escherichia coli.

Degree: Chemistry, 2010, University of North Carolina

The inside of the cell is a crowded and complex environment that is impossible to duplicate by studying proteins and other molecules in dilute solution. The effects of macromolecular crowding are potentially important to all cellular functions, but until recently studies have been conducted mostly in dilute solution. In-cell Nuclear Magnetic Resonance (NMR) spectroscopy is becoming an important tool in studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are seen in the supernatant surrounding the in-cell slurry. I examined how expression levels contribute to protein leakage. I also describe a device and system that provides a controlled environment for NMR experiments in living cells. I have utilized this device to study the expression of the natively disordered protein - synuclein, inside Escherichia coli. In the future, we hope to make progress in using this device to study proteins in eukaryotic cells with NMR. Advisors/Committee Members: Barnes, Christopher O., Pielak, Gary J., University of North Carolina at Chapel Hill.

Subjects/Keywords: College of Arts and Sciences; Department of Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Barnes, C. O. (2010). Protein in-cell NMR in Escherichia coli. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:86bacd50-a90b-43e9-aa46-0c286620f7b7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Barnes, Christopher O. “Protein in-cell NMR in Escherichia coli.” 2010. Thesis, University of North Carolina. Accessed January 25, 2021. https://cdr.lib.unc.edu/record/uuid:86bacd50-a90b-43e9-aa46-0c286620f7b7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Barnes, Christopher O. “Protein in-cell NMR in Escherichia coli.” 2010. Web. 25 Jan 2021.

Vancouver:

Barnes CO. Protein in-cell NMR in Escherichia coli. [Internet] [Thesis]. University of North Carolina; 2010. [cited 2021 Jan 25]. Available from: https://cdr.lib.unc.edu/record/uuid:86bacd50-a90b-43e9-aa46-0c286620f7b7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Barnes CO. Protein in-cell NMR in Escherichia coli. [Thesis]. University of North Carolina; 2010. Available from: https://cdr.lib.unc.edu/record/uuid:86bacd50-a90b-43e9-aa46-0c286620f7b7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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