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You searched for +publisher:"University of North Carolina" +contributor:("Adli, Mezher"). One record found.

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University of North Carolina

1. Adli, Mezher. Investigating the role of IKKε (epsilon) in cancer-associated NF-κB activity.

Degree: Biology, 2007, University of North Carolina

Nuclear Factor κB (NF-κB) has been studied extensively as an inducible transcriptional regulator of the immune and inflammatory responses. NF-κB activation downstream of LPS or cytokine stimulation is controlled by the IκB kinase complex which contains IKKα and IKKβ and NEMO. Significantly, the constitutive activity of NF-κB has been implicated as an important aspect of many cancer cells but mechanisms associated with this activity are poorly understood. An inducible kinase, IKK-i / IKKε, related to the catalytic forms of the IκB kinase, has been studied as an anti-viral, innate immune regulator through its ability to control the activity of the transcription factors IRF-3 and IRF-7. Here, we demonstrate that IKK-i / IKKε is expressed in a number of cancer cells and is involved in regulating NF-κB activity through its ability to control basal/constitutive, but not cytokine induced, p65/RelA phosphorylation at ser536, a modification proposed to contribute to the transactivation function of NF-κB. Knockdown of IKK-i / IKKε or expression of a S536A mutant form of p65 suppresses HeLa cell proliferation. The data indicate a role for IKK-i / IKKε in controlling proliferation of certain cancer cells through regulation of constitutive NF-κB activity. Moreover, microarray analysis of gene expression profiles in cancer cells with RNAi mediated IKKε knock-down demonstrated a number of genes that are highly regulated by IKKε. Among these genes, we were particularly interested in the Bcl-3 oncogene. After the observation that Bcl-3 gene expression is significantly reduced in microarray data following IKKε knock-down, we further confirmed and validated the microarray data by showing that overexpression of IKKε significantly upregulates Bcl-3 gene expression and that IKKε knock down downregulates it. Moreover, our data suggest that Bcl-3 gene expression is defective in IKKε knock-out MEF cells both at the protein level and at the message level. Promoter studies indicate that IKKε regulates Bcl-3 gene expression through NF-κB. We have also observed a significant correlation between IKKε expression and Bcl-3 expression in HCC tumors compared to adjacent tissue. My hypothesis is that IKKε regulation in cancer promotes oncogenic potential partly through the induced regulation of Bcl-3. Advisors/Committee Members: Adli, Mezher, Baldwin, Albert.

Subjects/Keywords: College of Arts and Sciences; Department of Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Adli, M. (2007). Investigating the role of IKKε (epsilon) in cancer-associated NF-κB activity. (Thesis). University of North Carolina. Retrieved from https://cdr.lib.unc.edu/record/uuid:1ba6ce64-e440-4012-8144-4559ef68836f

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Adli, Mezher. “Investigating the role of IKKε (epsilon) in cancer-associated NF-κB activity.” 2007. Thesis, University of North Carolina. Accessed September 26, 2020. https://cdr.lib.unc.edu/record/uuid:1ba6ce64-e440-4012-8144-4559ef68836f.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Adli, Mezher. “Investigating the role of IKKε (epsilon) in cancer-associated NF-κB activity.” 2007. Web. 26 Sep 2020.

Vancouver:

Adli M. Investigating the role of IKKε (epsilon) in cancer-associated NF-κB activity. [Internet] [Thesis]. University of North Carolina; 2007. [cited 2020 Sep 26]. Available from: https://cdr.lib.unc.edu/record/uuid:1ba6ce64-e440-4012-8144-4559ef68836f.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Adli M. Investigating the role of IKKε (epsilon) in cancer-associated NF-κB activity. [Thesis]. University of North Carolina; 2007. Available from: https://cdr.lib.unc.edu/record/uuid:1ba6ce64-e440-4012-8144-4559ef68836f

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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