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You searched for +publisher:"University of New South Wales" +contributor:("Khachigian, Levon, Faculty of Medicine, UNSW"). One record found.

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University of New South Wales

1. Zhang, Ning. Transcriptional regulation of PDGF receptor alpha in fibroblasts.

Degree: Centre for Vascular Research, 2011, University of New South Wales

Wound healing is a process of stimulating cell growth and proliferation to achieve the result of repair. This process is controlled by cytokines and growth factors which regulates the speed and efficiency of repair. Cytokines such as interleukin 1 beta (IL-1beta) and tumour necrosis factor alpha (TNF-alpha) regulates repair by inducing effects on inflammatory cells and control of growth factor expression. One down-stream factor which is regulated by cytokines is platelet-derived growth factor receptor alpha (PDGF-R-alpha). This receptor is important in the proliferation of fibroblasts and smooth muscle cells, important mediators in the process of wound healing.In this thesis, we discovered that following injury there is an induction in the expression of pro-inflammatory cytokines IL-1beta and TNF-alpha. This induction was seen after 24 and 48h exposure respectively. The effect of IL-1beta was seen to cause an increase in the proliferation of fibroblasts, whereas TNF-alpha had an inhibitory effect.The effect of IL-1beta was determined to be mediated through transcriptional activation of PDGF-R-alpha. Following IL-1beta release, there is the translocation and activation of Nuclear Factor kappa B (NF-kappaB) and Activating Transcription Factor 4 (ATF-4). These cooperatively interact on the promoter region of PDGF-R-alpha at the -531/-521 (NF-kappaB) and -259/-254 (ATF-4) positions and remove the presence of Histone deacetylases (HDAC), hence activating PDGF-R-alpha.On the other hand, TNF-alpha which showed an inhibitory effect on fibroblast proliferation decreased the expression of PDGF-R-alpha. This was achieved through activation of a transcription complex which contains the factors c-Fos, Interferon Regulatory Factor 1 (IRF-1) and Yin Yang 1 (YY-1). This complex recruits HDAC onto the promoter suppressing the expression of PDGF-R-alpha and its down-stream effects on proliferation.The opposing nature of pro-inflammatory cytokine mediated events suggests that these act as a natural balance in the control of wound healing efficacy. The elucidation of the pathways controlling wound healing, in this case the control of PDGF-R-alpha, will provide therapeutic targets to improve regeneration of skin and organs following injury. Advisors/Committee Members: Khachigian, Levon, Faculty of Medicine, UNSW.

Subjects/Keywords: PDGF receptor alpha; fibroblasts; interleukin 1 beta (IL-1beta); tumour necrosis factor alpha (TNF-alpha); receptor alpha (PDGF-R-alpha); wound healing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Zhang, N. (2011). Transcriptional regulation of PDGF receptor alpha in fibroblasts. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/51647 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10314/SOURCE02?view=true

Chicago Manual of Style (16th Edition):

Zhang, Ning. “Transcriptional regulation of PDGF receptor alpha in fibroblasts.” 2011. Doctoral Dissertation, University of New South Wales. Accessed October 20, 2019. http://handle.unsw.edu.au/1959.4/51647 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10314/SOURCE02?view=true.

MLA Handbook (7th Edition):

Zhang, Ning. “Transcriptional regulation of PDGF receptor alpha in fibroblasts.” 2011. Web. 20 Oct 2019.

Vancouver:

Zhang N. Transcriptional regulation of PDGF receptor alpha in fibroblasts. [Internet] [Doctoral dissertation]. University of New South Wales; 2011. [cited 2019 Oct 20]. Available from: http://handle.unsw.edu.au/1959.4/51647 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10314/SOURCE02?view=true.

Council of Science Editors:

Zhang N. Transcriptional regulation of PDGF receptor alpha in fibroblasts. [Doctoral Dissertation]. University of New South Wales; 2011. Available from: http://handle.unsw.edu.au/1959.4/51647 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10314/SOURCE02?view=true

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