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You searched for +publisher:"University of New Mexico" +contributor:("Lidke, Keith A."). Showing records 1 – 2 of 2 total matches.

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University of New Mexico

1. Malik, Michael David. HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING.

Degree: Physics & Astronomy, 2013, University of New Mexico

One of the challenges in studying protein interactions in live cells lies in the capacity to obtain both spatial and temporal information that is sufficient to extend existing knowledge of the dynamics and interactions, especially when tracking proteins at high density. Here we introduce a high-speed laser line-scanning hyperspectral microscope that is designed to track quantum dot labeled proteins at 27 frames/sec over an area of 28 um2 using 128 spectral channels spanning the range from 500 to 750 nm. This instrument simultaneously excites 8 species of quantum dots and employs a custom prism spectrometer and high speed EMCCD to obtain spectral information that is then used to distinguish and track individual probes at high density. These emitters are localized to within 10s of nm in each frame and reconstructed trajectories yield information of the protein dynamics and interactions. This manuscript describes the design, implementation, characterization, and application of a high-speed laser line-scanning hyperspectral microscope (HSM). The intended primary application is that of investigating the dynamics of transmembrane antibody receptors using quantum dot labeled immunoglobulin E (QD-IgE). Several additional examples demonstrate other advantages and applications of this method, including 3D hyperspectral imaging of live cells and hyperspectral superresolution imaging. Advisors/Committee Members: Lidke, Keith A., Lidke, Diane S., Thomas, James L., Prasad, Sudhakar.

Subjects/Keywords: confocal; hyperspectral; line-scanning; QD-IgE; quantum dot; SPT

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Malik, M. D. (2013). HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING. (Doctoral Dissertation). University of New Mexico. Retrieved from http://hdl.handle.net/1928/23133

Chicago Manual of Style (16th Edition):

Malik, Michael David. “HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING.” 2013. Doctoral Dissertation, University of New Mexico. Accessed June 04, 2020. http://hdl.handle.net/1928/23133.

MLA Handbook (7th Edition):

Malik, Michael David. “HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING.” 2013. Web. 04 Jun 2020.

Vancouver:

Malik MD. HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING. [Internet] [Doctoral dissertation]. University of New Mexico; 2013. [cited 2020 Jun 04]. Available from: http://hdl.handle.net/1928/23133.

Council of Science Editors:

Malik MD. HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING. [Doctoral Dissertation]. University of New Mexico; 2013. Available from: http://hdl.handle.net/1928/23133


University of New Mexico

2. Cugler Fiorante, Glauco Rogerio. Spatio-temporal circuits for imaging sensors.

Degree: Electrical and Computer Engineering, 2013, University of New Mexico

The first and second generations of infrared detectors—developed from the 1950s to the 1990s—were dominated by single pixel, linear, and staring small format, containing from 1 Kpixels to 100 Kpixels. In the past decade, the third-generation systems presented (a) large format (1 Mpixels to 16 Mpixels), (b) higher operating temperature (200 K to 250 K for MWIR, and 120 K to 150 K for LWIR), and (c) multicolor operation. The emphasis demanded for the next generation of devices is the incorporation of an enhanced functionality in the imagers—preferably at the pixel level—such as color, polarization, and dynamic range control, leading to a dramatic reduction in the size, complexity, and cost of infrared imaging systems. In this work, a new 96 x 96 pixel, 30 um pitch mixed-signal readout-integrated circuit (ROIC) with a pixel-level tunable bias control is demonstrated. The new ROIC is capable of providing a large-bias voltage in both polarities on each individual pixel, independently. These enhanced functionalities are achieved by modifying a capacitive transimpedance amplifier (CTIA) CMOS ROIC architecture. The unit cell electronic circuit was designed using 15 transistors and four capacitors and consists of the CTIA integrator—a two-stage, seven-transistor operational amplifier—one analog memory, one address selector, one reference recover switch, a sample-and-hold stage, an output buffer, and an output multiplex switch. Several test structures of individual devices and complete circuits were implemented on the test chip to characterize each one and to reconstruct the unit cell with discrete components, if necessary. Intending to test, characterize, and control the ITP-ROIC, an FPGA-based hardware and GUI software were developed to generate four analog and 26 digital output signals, with 87 adjustable parameters. In addition to the hardware, chip interconnection techniques were developed to grant nondestructive, flexible, quick interconnections for definition of new test setups. The test chip has been fabricated in TMSC 2P4M 0.35 um high-voltage CMOS technology by MOSIS. With 250 kHz of pixel clock and 57 ms of integration time, the acquired image presents 10 FPS. The ITP-ROIC has a bias voltage range of +/-5 V and an output voltage swing of +/-3.9 V. Advisors/Committee Members: Krishna, Sanjay, Zarkesh-Ha, Payman, Ramirez Fernandez, Francisco Javier, Lidke, Keith A., Han, Sang.

Subjects/Keywords: Readout Integrated Circuit; ROIC; FPGA-based testing system; Spatio-temporal tunable pixel; VLSI design; Mixed-signal design; Individual pixel voltage biasing; Infrared imaging ROIC; DWELL ROIC; p-i-n-i-p ROIC; IRFPA ROIC

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Cugler Fiorante, G. R. (2013). Spatio-temporal circuits for imaging sensors. (Doctoral Dissertation). University of New Mexico. Retrieved from http://hdl.handle.net/1928/23318

Chicago Manual of Style (16th Edition):

Cugler Fiorante, Glauco Rogerio. “Spatio-temporal circuits for imaging sensors.” 2013. Doctoral Dissertation, University of New Mexico. Accessed June 04, 2020. http://hdl.handle.net/1928/23318.

MLA Handbook (7th Edition):

Cugler Fiorante, Glauco Rogerio. “Spatio-temporal circuits for imaging sensors.” 2013. Web. 04 Jun 2020.

Vancouver:

Cugler Fiorante GR. Spatio-temporal circuits for imaging sensors. [Internet] [Doctoral dissertation]. University of New Mexico; 2013. [cited 2020 Jun 04]. Available from: http://hdl.handle.net/1928/23318.

Council of Science Editors:

Cugler Fiorante GR. Spatio-temporal circuits for imaging sensors. [Doctoral Dissertation]. University of New Mexico; 2013. Available from: http://hdl.handle.net/1928/23318

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