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You searched for +publisher:"University of Manitoba" +contributor:("Clouthier, Sharon (Biological Sciences) Anderson, Gary (Biological Sciences)"). One record found.

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University of Manitoba

1. Van Walleghem, Elissa. Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens).

Degree: Biological Sciences, 2013, University of Manitoba

Namao virus (NV) was associated with mortality in lake sturgeon Acipenser fulvescens reared as part of a conservation stocking program for this endangered species in Manitoba, Canada. The virus itself was large, doubly encapsidated and icosahedral-shaped. Phylogenetic analyses using the major capsid protein showed that NV and other epitheliotropic sturgeon nucleo-cytoplasmic large DNA viruses shared a common evolutionary past and formed a distinct evolutionary lineage within Megavirales. Three PCR tests were developed and their analytical performance was validated for detection of these viruses. Testing of wild sturgeon revealed that NV is endemic in the Nelson River water basin in Manitoba. Bath exposure resulted in transmission of NV to healthy sturgeon. The gills appeared to be the initial site of infection with virus persisting in the head skin tissue for up to 62 days. The molecular tests will be useful tools for disease management in sturgeon conservation stocking programs. Advisors/Committee Members: Clouthier, Sharon (Biological Sciences) Anderson, Gary (Biological Sciences) (supervisor), Docker, Margaret (Biological Sciences) Butler, Micheal (Microbiology) (examiningcommittee).

Subjects/Keywords: Namao virus; NCLDV; Lake Sturgeon; Endangered species; qPCR; Diagnostic PCR; Real-time PCR; Mimiviridae

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APA (6th Edition):

Van Walleghem, E. (2013). Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens). (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/30754

Chicago Manual of Style (16th Edition):

Van Walleghem, Elissa. “Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens).” 2013. Masters Thesis, University of Manitoba. Accessed December 08, 2019. http://hdl.handle.net/1993/30754.

MLA Handbook (7th Edition):

Van Walleghem, Elissa. “Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens).” 2013. Web. 08 Dec 2019.

Vancouver:

Van Walleghem E. Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens). [Internet] [Masters thesis]. University of Manitoba; 2013. [cited 2019 Dec 08]. Available from: http://hdl.handle.net/1993/30754.

Council of Science Editors:

Van Walleghem E. Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens). [Masters Thesis]. University of Manitoba; 2013. Available from: http://hdl.handle.net/1993/30754

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