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You searched for +publisher:"University of KwaZulu-Natal" +contributor:("Mwabvu, Tarombera"). Showing records 1 – 2 of 2 total matches.

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University of KwaZulu-Natal

1. Tinago, Tawanda. Genetic analyses of a Southern African millipede (Bicoxidens Attems 1928).

Degree: 2014, University of KwaZulu-Natal

Bicoxidens Attems, 1928 is an afrotropical millipede in the family Spirostreptidae which constitutes nine species. Based on the analysis of the male copulatory organs known as gonopods, Bicoxidens is monophyletic. However, gonopod morphology is central to millipede taxonomy, examination of gonopod morphology alone may not elucidate hidden genetic diversity thereby underestimating species richness in Bicoxidens. Analysis of genetic data as an additional source of taxonomic data has been noted to reveal genetic variation and flagged hidden species within millipedes. Hence there is a need to revise the morphological species definitions and phylogeny of Bicoxidens using both mitochondrial and nuclear DNA sequences. Furthermore, low vagility and habitat discontinuity may limit gene flow among Bicoxidens populations resulting differentiation and high genetic variation. In this regard, the sequences of the mitochondrial cytochrome oxidase 1 (COI) and 16S rRNA genes were used to delimit species, unravel hidden species and test the monophyly of Bicoxidens. In addition, the phylogeography and population genetic structure of B. flavicollis was investigated based on COI and 16S rRNA sequences. Genetic distances were computed using COI. Phylogenetic inferences were done based on Maximum likelihood and Bayesian inference analyses of the COI and 16S rRNA sequences individually and with the combined data set (COI+16S rRNA). The phylogeography and population genetic structure of B. flavicollis was inferred based on genetic diversity indices, population genetic differentiation estimate PhiPT, haplotype network analyses and maximum likelihood analyses of both COI and 16S rRNA. Bayesian population structure analyses was done using the COI data set only. Furthermore, Mantel’s isolation by distance among B. flavicollis specimens was tested. Interspecific genetic distances (> 0.075) based on the Juke-Cantor model supported the morphological species definitions in Bicoxidens. However, high intraspecific sequence differences in B. flavicollis (> 0.14), B. friendi (0.084) and B. brincki (> 0.188) suggest the presence of cryptic species. The possible presence of cryptic species was supported by the phylogenic analyses which recovered a paraphyletic Bicoxidens phylogram with B. flavicollis, B. brincki and B. friendi exhibiting divergent lineages. Bicoxidens flavicollis populations exhibited high genetic diversity and strong population differentiation which suggests restricted gene flow. Haplotype network and Maximum likelihood analyses revealed limited sharing of haplotypes and cryptic species in populations at Mazowe and Nyanga, respectively. Bicoxidens flavicollis populations were grouped in two four genetic clusters. Isolation by distance was insignificant among B. flavicollis specimens. The results support the utility of both COI and 16S rRNA in species delimitation in Bicoxidens. There is a growing body of evidence which also suggests presence of hidden species in B. flavicollis, B brincki and B friendi. Furthermore effort to uncover more… Advisors/Committee Members: Mwabvu, Tarombera (advisor), Macdonald, Angus Hector Harold (advisor).

Subjects/Keywords: Microbiology.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Tinago, T. (2014). Genetic analyses of a Southern African millipede (Bicoxidens Attems 1928). (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/12403

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Tinago, Tawanda. “Genetic analyses of a Southern African millipede (Bicoxidens Attems 1928).” 2014. Thesis, University of KwaZulu-Natal. Accessed February 17, 2019. http://hdl.handle.net/10413/12403.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Tinago, Tawanda. “Genetic analyses of a Southern African millipede (Bicoxidens Attems 1928).” 2014. Web. 17 Feb 2019.

Vancouver:

Tinago T. Genetic analyses of a Southern African millipede (Bicoxidens Attems 1928). [Internet] [Thesis]. University of KwaZulu-Natal; 2014. [cited 2019 Feb 17]. Available from: http://hdl.handle.net/10413/12403.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tinago T. Genetic analyses of a Southern African millipede (Bicoxidens Attems 1928). [Thesis]. University of KwaZulu-Natal; 2014. Available from: http://hdl.handle.net/10413/12403

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of KwaZulu-Natal

2. Nxele, Thembeka Clara. A taxonomic revision of Tritogenia Kinberg, 1867 and Michalakus Plisko, 1996 (Oligochaeta, Tritogeniidae) occurring in KwaZulu-Natal Midlands, South Africa, based on morphological and DNA sequence data.

Degree: Microbiology, 2014, University of KwaZulu-Natal

The conservation and monitoring of biodiversity depends on the knowledge of species identity and distribution. Southern Africa has a rich and characteristic megadrile fauna. Most of the fauna show high levels of endemism with closely related species often separated by subtle morphological characters. Grasslands and forests of South Africa have a diverse terrestrial earthworm fauna, but up to date systematic studies of most taxa are incomplete. Such studies are an opportunity to contribute to understanding evolutionary processes and to provide information for conservation. The genera Tritogenia and Michalakus occur in grasslands and forests in north-eastern part of South Africa in the KwaZulu-Natal province. This study investigated the taxonomic validity of the Tritogenia and Michalakus species in the KwaZulu-Natal Midlands. Ten species of Tritogenia and one of Michalakus are known from this region, with species descriptions based on morphological characters. In this study integrative taxonomy is employed, with both morphological and molecular data used to assess the reliability of traditional morphology-based techniques and the relationships among these species. Detailed comparative morphological observations from fresh Tritogenia material revealed a synonym (Tritogenia soleata Plisko, 1997 = Tritogenia shawi Plisko & Zicsi 1991). To gain further evidence for species level taxonomy and distribution patterns, a molecular phylogeny was constructed based on mitochondrial genes cytochrome c oxidase subunit I (COI) and 16S rRNA. A total of 146 individuals were sequenced for COI from 22 localities and 43 were sub-sampled for 16S rDNA. In most cases, the morphological and molecular data are congruent. The molecular data revealed that the genus Tritogenia is not monophyletic as previously thought. Michalakus initus Plisko 1996 nests within Tritogenia and this finding is observed in both morphological and molecular data. Tritogenia shawi is a cluster with the outgroup species not with other Midlands Tritogenia species. These findings demonstrate the value of using integrative taxonomy in highlighting/revealing the complexities of earthworm fauna in South Africa. The combined morphological and molecular data, though not well supported, ancestral character state reconstructions are generally in agreement with the morphological data in terms of which characters were useful in phylogeny construction. Advisors/Committee Members: Mwabvu, Tarombera (advisor), Plisko, Jagwiga Danuta (advisor), Willows-Munro, Sandi (advisor).

Subjects/Keywords: Microbiology.

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Nxele, T. C. (2014). A taxonomic revision of Tritogenia Kinberg, 1867 and Michalakus Plisko, 1996 (Oligochaeta, Tritogeniidae) occurring in KwaZulu-Natal Midlands, South Africa, based on morphological and DNA sequence data. (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/12439

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Nxele, Thembeka Clara. “A taxonomic revision of Tritogenia Kinberg, 1867 and Michalakus Plisko, 1996 (Oligochaeta, Tritogeniidae) occurring in KwaZulu-Natal Midlands, South Africa, based on morphological and DNA sequence data.” 2014. Thesis, University of KwaZulu-Natal. Accessed February 17, 2019. http://hdl.handle.net/10413/12439.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Nxele, Thembeka Clara. “A taxonomic revision of Tritogenia Kinberg, 1867 and Michalakus Plisko, 1996 (Oligochaeta, Tritogeniidae) occurring in KwaZulu-Natal Midlands, South Africa, based on morphological and DNA sequence data.” 2014. Web. 17 Feb 2019.

Vancouver:

Nxele TC. A taxonomic revision of Tritogenia Kinberg, 1867 and Michalakus Plisko, 1996 (Oligochaeta, Tritogeniidae) occurring in KwaZulu-Natal Midlands, South Africa, based on morphological and DNA sequence data. [Internet] [Thesis]. University of KwaZulu-Natal; 2014. [cited 2019 Feb 17]. Available from: http://hdl.handle.net/10413/12439.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nxele TC. A taxonomic revision of Tritogenia Kinberg, 1867 and Michalakus Plisko, 1996 (Oligochaeta, Tritogeniidae) occurring in KwaZulu-Natal Midlands, South Africa, based on morphological and DNA sequence data. [Thesis]. University of KwaZulu-Natal; 2014. Available from: http://hdl.handle.net/10413/12439

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.