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University of Illinois – Urbana-Champaign
1.
Jankelow, Aaron Mitchell.
Simulation model of a microfluidic point of care biosensor for electrical enumeration of blood cells.
Degree: MS, Bioengineering, 2018, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/101193 
► Point of care microfluidic devices provide many opportunities for improving the diagnosis of a number of illnesses. They can provide a speedy, quantitative assay in…
(more)
▼ Point of care microfluidic devices provide many opportunities for improving the diagnosis of a number of illnesses. They can provide a speedy, quantitative assay in the form of an easy to use portable platform. By using Finite Element Analysis software to model and simulate these microfluidic devices, we can further optimize and improve on the design of such devices. In this work we will use such software in order to model an electrical counting chamber that would be implemented in such a device. This chamber utilizes the coulter counting principle to measure the change in impedance caused when a bead or a cell passes over a series of electrodes. By utilizing the signals to count the number of cells coming into and out of a capture chamber that targets a specific antigen, we can obtain a quantitative measure of how many cells or beads were expressing the target antigen and use this for a diagnosis. First the simulation was tuned to be able to produce the characteristic bipolar pulse when a cell passed over the electrodes. Then by varying elements such as bead size, input voltage, bead composition and electrode placement and recording the results we can use this model to help further refine and optimize this device by giving us a quantitative model that will allow us to better understand how changing such variables will alter the signal received from the device and thus allow us a better understanding of the best way to get a clearer signal.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: Finite Element Analysis; Sepsis; microfluidics; COMSOL; coulter counter
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APA (6th Edition):
Jankelow, A. M. (2018). Simulation model of a microfluidic point of care biosensor for electrical enumeration of blood cells. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/101193
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jankelow, Aaron Mitchell. “Simulation model of a microfluidic point of care biosensor for electrical enumeration of blood cells.” 2018. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/101193.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jankelow, Aaron Mitchell. “Simulation model of a microfluidic point of care biosensor for electrical enumeration of blood cells.” 2018. Web. 17 Apr 2021.
Vancouver:
Jankelow AM. Simulation model of a microfluidic point of care biosensor for electrical enumeration of blood cells. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/101193.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jankelow AM. Simulation model of a microfluidic point of care biosensor for electrical enumeration of blood cells. [Thesis]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/101193
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
2.
Cvetkovic, Caroline.
The development of a skeletal muscle bio-actuator using 3-D stereolithography.
Degree: MS, 0408, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/44448
► Over the past decade, a new scientific discipline has emerged, integrating mechanics with biology to create complex engineered living systems. The building blocks – different…
(more)
▼ Over the past decade, a new scientific discipline has emerged, integrating mechanics with biology to create complex engineered living systems. The building blocks – different cell types in an instructive environment – can be assembled in various ways to promote the emergence (or natural evolution and interaction) of the cells in a system with well-defined functionality. These functions could include sensing, information processing, protein expression, and actuation, among countless others.
This Thesis presents a novel cellular system capable of actuation and fabricated using cells and hydrogels. A stereolithographic 3D printing technique (SLA) was used to create a hydrogel backbone made of a beam connecting two pillars that supports a muscle strip created from skeletal muscle cells in a fibrin-based matrix. The entire device is termed a “bio-bot,” or biological robot. Contraction of the cells within the muscle strip produced enough force to move the pillars and displace the bio-bot on a surface in a liquid medium. This Thesis is focused on the development and characterization (mechanical and biological) of the skeletal muscle-based biological actuator.
The use of the SLA allowed for easy modifications of the polymerized part’s geometry and material properties. Increasing the energy dose of polymerization produced a stiffer beam that restricted bending and increased the passive tension in the muscle strip. 15-19 days after cell seeding, the bio-bots displayed spontaneous contraction that resulted in a net displacement of up to ~6 mm in 10 minutes. During this time span, a maximum velocity of over 1890 μm/min was achieved. Future plans are focused on controlling the activity of the bio-bot using optogenetics.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: skeletal muscle; bio-actuator; stereolithography
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APA (6th Edition):
Cvetkovic, C. (2013). The development of a skeletal muscle bio-actuator using 3-D stereolithography. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/44448
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cvetkovic, Caroline. “The development of a skeletal muscle bio-actuator using 3-D stereolithography.” 2013. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/44448.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cvetkovic, Caroline. “The development of a skeletal muscle bio-actuator using 3-D stereolithography.” 2013. Web. 17 Apr 2021.
Vancouver:
Cvetkovic C. The development of a skeletal muscle bio-actuator using 3-D stereolithography. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/44448.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cvetkovic C. The development of a skeletal muscle bio-actuator using 3-D stereolithography. [Thesis]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/44448
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
3.
Rivera, Jose.
Graphene supported hafnium oxide nanopores for DNA sensing.
Degree: MS, 0408, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/44455
► The completion of the Human Genome Project in 2001 has served as both an inspiration and a challenge for researchers in the past decade. Understanding…
(more)
▼ The completion of the Human Genome Project in 2001 has served as both an inspiration and a challenge for researchers in the past decade. Understanding the genetic makeup of organisms is crucial for early disease detection, one of the driving forces behind personalized medicine. In this work, state-of-the-art sequencing technologies are reviewed and compared with next-generation sequencing technologies. In particular, solid-state nanopores are investigated and recent developments in the field are discussed. The interdisciplinary effort from researchers to establish solid-state nanopores as a viable sequencing platform is thriving on multiple fronts including surface charge engineering for DNA capture and conductance modulation in nanopores, nanowire transistors for localized detection, ultra-thin membrane fabrication using graphene, and the exploration of alternative nanopore materials for biosensing applications. In this work, the development of a new solid-state nanopore sensor consisting of hafnium oxide suspended by functionalized graphene is reported along with DNA transport properties and dielectric characterization of the film in solution.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: nanopores; DNA sensing; graphene; solid-state nanopores; graphene membrane; transmission electron microscopy (TEM)
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APA ·
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MLA ·
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Export
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APA (6th Edition):
Rivera, J. (2013). Graphene supported hafnium oxide nanopores for DNA sensing. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/44455
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rivera, Jose. “Graphene supported hafnium oxide nanopores for DNA sensing.” 2013. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/44455.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rivera, Jose. “Graphene supported hafnium oxide nanopores for DNA sensing.” 2013. Web. 17 Apr 2021.
Vancouver:
Rivera J. Graphene supported hafnium oxide nanopores for DNA sensing. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/44455.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rivera J. Graphene supported hafnium oxide nanopores for DNA sensing. [Thesis]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/44455
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
4.
Hassan, Umer.
Microfluidic sensor for white blood cell counting and flow metering.
Degree: MS, 1200, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/44488
► Cell counting finds many applications in diagnostics of many diseases. One of the most common tests recommended by the physicians is Complete Blood Cell Count…
(more)
▼ Cell counting finds many applications in diagnostics of many diseases. One of the most common tests recommended by the physicians is Complete Blood Cell Count (CBC). For example, there is a decrease in the platelet count in case of Dengue fever. Red blood cells decrease in the case of anemia. CD4 T cells decrease in the case of HIV/AIDS. Cell counting in general, and ability to count the specific cells, would greatly help in clinical diagnostics. Currently, flow cytometers are used for this purpose, but they have not been able to penetrate in the resource-limited settings around the world because of being expensive and because they require trained technicians to operate. Over many years, people have developed microfluidic devices for cell counting, which could provide a portable and economical solution to the problem of cell counting at point-of-care.
In this report, we present a technique for counting the white blood cells and differentiating some of its sub-types within a microfluidic device. Starting with the whole blood, the red blood cells are lysed by saponin and formic acid. Quenching solution composed of phosphate buffer saline is infused in the device to halt the lysing process and maintain the pH of the solution. The remaining white blood cells are then passed across micro-fabricated electrodes within a microfluidic channel. The impedance is measured at 303 kHz and 1.7 MHz signals. The height of the pulse is proportional to the size of the cell. By selecting the appropriate threshold, the number of the cells can be calculated, and white blood cell sub-types can be differentiated based on their size.
Lysed blood flow metering is also possible using the same setup. The width of the pulse for each passage of the cell through the electrode is proportional to the speed of the cell. By investigating the pulse width, the flow rate can be monitored. Similarly, with increasing flow rates, the baseline amplitude measured in between the electrodes is also proportional to the flow rate.
This device has the potential for use in counting specific types of white blood cells. In particular, we are currently characterizing the device for counting of CD4+ and CD8+ T lymphocytes, which are the primary diagnostics biomarkers for HIV/AIDS.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: Microfluidic; Cell counting; Electrical flow metering; Complete blood cell count; White blood cell counting
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hassan, U. (2013). Microfluidic sensor for white blood cell counting and flow metering. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/44488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hassan, Umer. “Microfluidic sensor for white blood cell counting and flow metering.” 2013. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/44488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hassan, Umer. “Microfluidic sensor for white blood cell counting and flow metering.” 2013. Web. 17 Apr 2021.
Vancouver:
Hassan U. Microfluidic sensor for white blood cell counting and flow metering. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/44488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hassan U. Microfluidic sensor for white blood cell counting and flow metering. [Thesis]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/44488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
5.
Damhorst, Gregory L.
A liposome-based ion release impedance sensor for HIV detection at the point-of-care.
Degree: MS, 0408, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/44806
► Many individuals living in remote and resource-limited regions around the world face a barrier to adequate medical care due to the unavailability of diagnostic instrumentation…
(more)
▼ Many individuals living in remote and resource-limited regions around the world face a barrier to adequate medical care due to the unavailability of diagnostic instrumentation to perform standard measurements which diagnose disease and inform treatment. Factors which limit the availability of diagnostics include both the costs of instrumentation and per-test costs, inadequate portability, the requirement for a laboratory facility, the time required to perform the test, and a need for highly-trained personnel to operate the instrumentation. While a wide variety of approaches primarily based on innovations in micro- and nanotechnology have been reported toward the point-of-care sensing of biological entities, none has yet emerged as a comprehensive solution to the fundamental problems of diagnostic testing in resource-limited settings. In strides toward a platform for low-cost, rapid, easy-to-use point-of-care diagnostics, we present an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet become permeable for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements and the detection of HIV, which we have selected as a problem to target in the development of this platform, which ultimately can be applied more broadly to other biomolecules and antigens. By showing the detection of viruses on this biosensor platform, we have demonstrate a proof-of-concept which will be further optimized and advanced toward quantitative determination of viral load from whole blood specimens.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: biosensors; point-of-care diagnostics; HIV/AIDS; pathogen sensing; microfluidics; impedance sensing
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Damhorst, G. L. (2013). A liposome-based ion release impedance sensor for HIV detection at the point-of-care. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/44806
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Damhorst, Gregory L. “A liposome-based ion release impedance sensor for HIV detection at the point-of-care.” 2013. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/44806.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Damhorst, Gregory L. “A liposome-based ion release impedance sensor for HIV detection at the point-of-care.” 2013. Web. 17 Apr 2021.
Vancouver:
Damhorst GL. A liposome-based ion release impedance sensor for HIV detection at the point-of-care. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/44806.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Damhorst GL. A liposome-based ion release impedance sensor for HIV detection at the point-of-care. [Thesis]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/44806
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
6.
Keller, Katrina.
Using resonant MEMS pedestal sensors and filtering techniques to determine the growth curve of MDA-MB-231, metastatic breast cancer cells.
Degree: MS, 0408, 2014, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/46940
► Studying cells at the individual level is incredibly important for understanding underlying mechanisms for growth, cell cycle control, cell mass, cell adhesion to surfaces, and…
(more)
▼ Studying cells at the individual level is incredibly important for understanding underlying mechanisms for growth, cell cycle control, cell mass, cell adhesion to surfaces, and ultimately the nature of cancer cells. A resonant pedestal sensor was used to study single, adherent cells non-invasively. This measurement tool was preferred over other methods because the mass uniformity of its surface is independent of the location of the cell. Images were taken at each time point to aid in the data processing. This resonant pedestal sensor was fabricated in a cleanroom facility and used to measure the mass of an individual MDA-MB-231 (metastatic breast cancer) cell. The noisy mass measurements were subjected to two different processing methods: Savitzky-Golay and Moving Average. Our main goal in performing these two techniques was to determine the most appropriate processing method for our data. We found the Savitzky-Golay method to be more favorable than the moving average to interpret the MDA-MB-231 cell growth curves. Once outlier exclusion was performed on the data, a positive linear relationship was visible and we were able to calculate the percent change of this cell type: 1% of cell mass is increased per hour.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: Microelectromechanical systems (MEMS); Cancer
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APA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
Keller, K. (2014). Using resonant MEMS pedestal sensors and filtering techniques to determine the growth curve of MDA-MB-231, metastatic breast cancer cells. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/46940
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Keller, Katrina. “Using resonant MEMS pedestal sensors and filtering techniques to determine the growth curve of MDA-MB-231, metastatic breast cancer cells.” 2014. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/46940.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Keller, Katrina. “Using resonant MEMS pedestal sensors and filtering techniques to determine the growth curve of MDA-MB-231, metastatic breast cancer cells.” 2014. Web. 17 Apr 2021.
Vancouver:
Keller K. Using resonant MEMS pedestal sensors and filtering techniques to determine the growth curve of MDA-MB-231, metastatic breast cancer cells. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2014. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/46940.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Keller K. Using resonant MEMS pedestal sensors and filtering techniques to determine the growth curve of MDA-MB-231, metastatic breast cancer cells. [Thesis]. University of Illinois – Urbana-Champaign; 2014. Available from: http://hdl.handle.net/2142/46940
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
7.
Collens, Mitchell.
2D and 3D patterning of molecules and cells.
Degree: MS, 0408, 2012, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/31977
► This thesis is designed to help understand the need for patterning molecules and cells for uses in medicine and engineering. Our goal is to be…
(more)
▼ This thesis is designed to help understand the need for patterning molecules and cells for uses in medicine and engineering. Our goal is to be able to understand how cells and biological systems develop and how we can use design to harness cellular function. Two approaches are taken to pattern multiple cell types, and build 2D and 3D substrates. First, a combination of ink jet printing and silane chemistry are used to develop a method for high throughput patterning and analysis of Hippocampal Neurons on glass. Cell adhesive synthetic Poly-L-Lysine patterns are built juxtaposed from a non-adhesive silane background and control the distribution and growth of Hippocampal Neurons. Results from large scale patterning and analysis (44000 neurons/sample) show that neural somata align to adhesive spots or lines, neurites are restricted to patterned areas, and axonal branching is induced on growth promoting areas. In addition to the 2D patterning of neurons, a stereolithography apparatus is used to build CAD designed porous 3D hydrogels out of photoactivated poly (ethylene glycol). Hydrogels are built on glass slides that have been microcontact printed with lines of acrylic-fibronectin: during polymerization, fibronectin patterns cross-link to the surface of hydrogels forming cell adhesive patterns. Results show that fibroblasts align and spread on patterns, cardiomyocytes align and maintain their ability to autonomously contract and relax, and C2C12 muscle cells align and differentiate from myoblasts to multinucleated myotubes.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: Patterning; Stereolithography; Neurons; Cardiac Cells; Biological machines; Hydrogel; 3D
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Collens, M. (2012). 2D and 3D patterning of molecules and cells. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/31977
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Collens, Mitchell. “2D and 3D patterning of molecules and cells.” 2012. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/31977.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Collens, Mitchell. “2D and 3D patterning of molecules and cells.” 2012. Web. 17 Apr 2021.
Vancouver:
Collens M. 2D and 3D patterning of molecules and cells. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2012. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/31977.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Collens M. 2D and 3D patterning of molecules and cells. [Thesis]. University of Illinois – Urbana-Champaign; 2012. Available from: http://hdl.handle.net/2142/31977
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
8.
Ornob, Akid.
Loop mediated isothermal amplification based detection of equine respiratory pathogens using a portable, smartphone-based setup.
Degree: MS, Bioengineering, 2017, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/99435
► New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that…
(more)
▼ New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a multiplexed platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld “cradle” that interfaces the phone with a silicon-based microfluidic chip embedded within a credit card-sized cartridge. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrate the ability of the system to utilize a single 15 µL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. Our approach utilizes loop mediated isothermal amplification (LAMP) reagents pre-deposited into distinct lanes of the microfluidic chip, which, when exposed to target nucleic acid sequences from the test sample, generates fluorescent products that, when excited by appropriately selected light emitting diodes (LEDs) are visualized and automatically analyzed by a software application running on the smartphone microprocessor. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments. Assay information is combined with information from the cartridge and the patient to populate a cloud-based database for epidemiological reporting of test results.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: Point-of-care diagnostics; Infectious diseases; Biosensors; Global health; Lab on a chip
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ornob, A. (2017). Loop mediated isothermal amplification based detection of equine respiratory pathogens using a portable, smartphone-based setup. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/99435
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ornob, Akid. “Loop mediated isothermal amplification based detection of equine respiratory pathogens using a portable, smartphone-based setup.” 2017. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/99435.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ornob, Akid. “Loop mediated isothermal amplification based detection of equine respiratory pathogens using a portable, smartphone-based setup.” 2017. Web. 17 Apr 2021.
Vancouver:
Ornob A. Loop mediated isothermal amplification based detection of equine respiratory pathogens using a portable, smartphone-based setup. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2017. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/99435.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ornob A. Loop mediated isothermal amplification based detection of equine respiratory pathogens using a portable, smartphone-based setup. [Thesis]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/99435
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
9.
Ganguli, Anurup.
Spatially mapped gene expression analysis from tissue.
Degree: MS, Bioengineering, 2016, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/90974
► Spatial gene expression analysis platforms have been widely used for a variety of applications ranging from pathogen detection to the analysis of spatial variation of…
(more)
▼ Spatial gene expression analysis platforms have been widely used for a variety of applications ranging from pathogen detection to the analysis of spatial variation of an mRNA in a tissue section. Traditionally, this is accomplished by using in situ polymerase chain reaction (in situ PCR) or in situ hybridization (ISH). In addition, laser capture microdissection (LCM) followed by RT-qPCR of the locally captured tissue has also been used to study these spatial variations at a molecular level. But, all of the above techniques are plagued with different issues such as low sensitivity in the case of ISH, low reproducibility and long experimental run time for in situ PCR, and long sample acquisition and purification times for the LCM based techniques. This calls for a method that can reliably, rapidly and with high sensitivity perform spatial gene expression analysis starting from a tissue sample. We present a novel approach that combine microfabrication techniques with reverse transcription-loop mediated isothermal amplification (RT-LAMP) to achieve this goal. This novel technique uses a micro-fabricated chip with an array of micro-wells with knife-like sharp well edges to assist in division of a tissue cryosection into small pieces in a process we call "tissue pixelation". The array consists of over five thousand 100um (side length) pyramidal wells with a volume of ~ 175pL each. Following the above tissue transfer step, reagents are loaded onto the chip into the individual wells using a parallel loading process and independent picoliter volume RT-LAMP reactions are performed in each well. Towards this end, we have completed and characterized the chip fabrication, tissue pixelation and picoliter volume reagent loading on chip steps. In addition to this, we have also designed and characterized a novel RT-LAMP reaction for topoisomerase II alpha (TOP2A) mRNA biomarker for LNCaP prostate cancer cells.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: Spatial gene expression analysis; RT-LAMP; lab-on-a-chip; microwell array
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Export
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APA (6th Edition):
Ganguli, A. (2016). Spatially mapped gene expression analysis from tissue. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/90974
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ganguli, Anurup. “Spatially mapped gene expression analysis from tissue.” 2016. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/90974.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ganguli, Anurup. “Spatially mapped gene expression analysis from tissue.” 2016. Web. 17 Apr 2021.
Vancouver:
Ganguli A. Spatially mapped gene expression analysis from tissue. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2016. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/90974.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ganguli A. Spatially mapped gene expression analysis from tissue. [Thesis]. University of Illinois – Urbana-Champaign; 2016. Available from: http://hdl.handle.net/2142/90974
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
10.
Ghonge, Tanmay.
Microfluidic particle tracking technique towards white blood cell subtype counting and serum protein quantification.
Degree: MS, Bioengineering, 2016, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/92875
► Microfluidic technologies have gained wide acceptance in the past decade as diagnostics tools in clinical setting world-wide. This is primarily due to the fact that…
(more)
▼ Microfluidic technologies have gained wide acceptance in the past decade as diagnostics tools in clinical setting world-wide. This is primarily due to the fact that microfluidic technologies enable rapid, quantitative assays from small amount of physiological sample in an easy-to-use, portable platform. In this work, we will describe a microfluidic technique that can be built upon to count white blood cell subtypes or serum protein from a drop of blood. Traditionally, researchers have counted white blood cell subtypes by capturing them. However, an elegant and more accurate way to do the same is by exploiting the transitory interactions between the antigen on the surface of the cell and a cognate antibody. Cells expressing the antigen of interest will take longer to traverse a microchannel which has been coated with a cognate antibody compared to the cells which don't express that antigen. To our knowledge, no microfluidic assay exists which can rapidly count cells using this principle. Towards this end, we have developed a repeatable experimental technique to control the transit time and the order of particles in a microchannel. To least affect the uniformity of transit time, we have also optimized the geometry of pillars in the microchannel on which antibodies are functionalized.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor).
Subjects/Keywords: Microfluidics
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APA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Ghonge, T. (2016). Microfluidic particle tracking technique towards white blood cell subtype counting and serum protein quantification. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/92875
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ghonge, Tanmay. “Microfluidic particle tracking technique towards white blood cell subtype counting and serum protein quantification.” 2016. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/92875.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ghonge, Tanmay. “Microfluidic particle tracking technique towards white blood cell subtype counting and serum protein quantification.” 2016. Web. 17 Apr 2021.
Vancouver:
Ghonge T. Microfluidic particle tracking technique towards white blood cell subtype counting and serum protein quantification. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2016. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/92875.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ghonge T. Microfluidic particle tracking technique towards white blood cell subtype counting and serum protein quantification. [Thesis]. University of Illinois – Urbana-Champaign; 2016. Available from: http://hdl.handle.net/2142/92875
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
11.
Raman, Ritu.
3D microfabrication of biological machines.
Degree: MS, Mechanical Engineering, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/100422
► The burgeoning field of additive manufacturing, or “3D printing”, centers on the idea of creating three-dimensional objects from digital models. While conventional manufacturing approaches rely…
(more)
▼ The burgeoning field of additive manufacturing, or “3D printing”, centers on the idea of creating three-dimensional objects from digital models. While conventional manufacturing approaches rely on modifying a base material via subtractive processes such as drilling or cutting, 3D printing creates three-dimensional objects through successive deposition of two- dimensional layers. By enabling rapid fabrication of complex objects, 3D printing is revolutionizing the fields of engineering design and manufacturing. This thesis details the development of a projection-based stereolithographic 3D printing apparatus capable of high- resolution patterning of living cells and cell signals dispersed in an absorbent hydrogel polymer matrix in vitro. This novel enabling technology can be used to create model cellular systems that lead to a quantitative understanding of the way cells sense, process, and respond to signals in their environment.
The ability to pattern cells and instructive biomaterials into complex 3D patterns has many applications in the field of tissue engineering, or “reverse engineering” of cellular systems that replicate the structure and function of native tissue. While the goal of reverse engineering native tissue is promising for medical applications, this idea of building with biological components concurrently brings about a new discipline: “forward engineering” of biological machines and systems. In addition to rebuilding existing systems with cells, this technology enables the design and forward engineering of novel systems that harness the innate dynamic abilities of cells to self-organize, self-heal, and self-replicate in response to environmental cues. This thesis details the development of skeletal and cardiac muscle based bioactuators that can sense external electrical and optical signals and demonstrate controlled locomotive behavior in response to them. Such machines, which can sense, process, and respond to signals in a dynamic environment, have a myriad array of applications including toxin neutralization and high throughput drug testing in vitro and drug delivery and programmable tissue engineered implants in vivo.
A synthesis of two fields, 3D printing and tissue engineering, has brought about a new discipline: using microfabrication technologies to forward engineer biological machines and systems capable of complex functional behavior. By introducing a new set of “building blocks” into the engineer’s toolbox, this new era of design and manufacturing promises to open up a field of research that will redefine our world.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Saif%2C%20Taher%22%29&pagesize-30">Saif, Taher (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Saif%2C%20Taher%22%29&pagesize-30">Saif, Taher (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member).
Subjects/Keywords: bioactuator, tissue engineering, skeletal muscle, cardiac muscle, optogenetics; bionanotechnology; microfabrication; 3d printing
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Raman, R. (2013). 3D microfabrication of biological machines. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/100422
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Raman, Ritu. “3D microfabrication of biological machines.” 2013. Thesis, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/100422.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Raman, Ritu. “3D microfabrication of biological machines.” 2013. Web. 17 Apr 2021.
Vancouver:
Raman R. 3D microfabrication of biological machines. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/100422.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Raman R. 3D microfabrication of biological machines. [Thesis]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/100422
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
12.
Dorvel, Brian.
Design and optimization of ultrathin silicon field effect transistor's for sensitive, electronic-based detection of biological analytes.
Degree: PhD, 0319, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/44465
► Noncommunicable diseases (NCD) are currently the leading cause of death worldwide. Over 57 million deaths occur globally each year, with close to 36 million of…
(more)
▼ Noncommunicable diseases (NCD) are currently the leading cause of death worldwide. Over 57 million deaths occur globally each year, with close to 36 million of them attributed to NCD’s, and 80% of those in low and middle income countries. Most of these were due to such chronic illnesses as cancer, cardiovascular disease, diabetes, and lung disease. Moreover, the prevalence of these diseases is rising fastest in low-income regions which have little resources to combat these large, yet avoidable costs. In particular, over 1.6 million cases of cancer are caused each year in the United States, with nearly 600,000 of these cases being fatal. Cancer is an uncontrolled growth and spread of abnormal cells in the body, and unfortunately, can exist in many different cell types. The complexity in the causes of cancer has made it tougher to diagnose since several factors may weight into its prevalence such as: genetic factors, lifestyle factors, certain types of infections, and different environmental exposures. As a result, the protocols for the most cost-effective intervention are available across four main approaches to cancer prevention and control: primary prevention, early detection, treatment, and palliative care. Early diagnosis based on awareness of early signs and symptoms and, if affordable, population-based screening improves survival, particularly for breast, cervical, colorectal, skin and oral cancers.
If primary prevention of cancer fails, secondary prevention (early detection) may be the difference between irreversible spread of a malignant cancer, and the patient’s survival. Early detection commonly refers to the diagnosis of a disease before individuals show obvious signs or symptoms of illness. With cancer, RNA and protein biomarkers of cells are currently assayed to determine their serums level and if they have deviated from the normal ranges. However, these assays commonly require large centralized lab facilities, frequent monitoring during treatment, and expensive equipment and/or supplies. This has led to point-of-care diagnostics becoming a $16 billion global market, aimed at miniaturizing technology and making it cost-effective for individual patient testing and treatment without the use of centralized lab facilities. A main point-of-care testing platform being pursued utilizes Complementary Metal Oxide Semiconductor (CMOS) technology. CMOS-based products can enable clinical tests to be conducted in a fast, simple, safe, and reliable manner, with improved sensitivities. Moreover, CMOS products offer portability and low power consumption, in large part due to the explosion in the semiconductor and communications markets.
Silicon nanowires are of great interest for point-of-care testing as they are a CMOS compatible structure, require the use of no labels, and are highly sensitive to the binding of molecules to their surfaces. This is due to the large surface area to volume ratio afforded to nanowires. Moreover, arrays of silicon nanowires have demonstrated multiplexed, label-free sensing of cancer…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Olsen%2C%20Gary%20J.%22%29&pagesize-30">Olsen, Gary J. (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Kong%2C%20Hyun%20Joon%22%29&pagesize-30">Kong, Hyun Joon (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Liu%2C%20Gang%20Logan%22%29&pagesize-30">Liu, Gang Logan (committee member).
Subjects/Keywords: nanowire; self-assembled monolayer; Field Effect Transistor (FET); biosensor; micro RNA; immunoglobulin, sensing
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dorvel, B. (2013). Design and optimization of ultrathin silicon field effect transistor's for sensitive, electronic-based detection of biological analytes. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/44465
Chicago Manual of Style (16th Edition):
Dorvel, Brian. “Design and optimization of ultrathin silicon field effect transistor's for sensitive, electronic-based detection of biological analytes.” 2013. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/44465.
MLA Handbook (7th Edition):
Dorvel, Brian. “Design and optimization of ultrathin silicon field effect transistor's for sensitive, electronic-based detection of biological analytes.” 2013. Web. 17 Apr 2021.
Vancouver:
Dorvel B. Design and optimization of ultrathin silicon field effect transistor's for sensitive, electronic-based detection of biological analytes. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/44465.
Council of Science Editors:
Dorvel B. Design and optimization of ultrathin silicon field effect transistor's for sensitive, electronic-based detection of biological analytes. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/44465
University of Illinois – Urbana-Champaign
13.
Hassan, Umer.
A microfluidic biosensor to electrically enumerate blood cells at point-of-care for infectious disease diagnosis and management.
Degree: PhD, Electrical & Computer Engr, 2015, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/78743
► Cell counting finds many applications in diagnostics of many diseases. One of the most common tests recommended by the physicians is complete blood cell count…
(more)
▼ Cell counting finds many applications in diagnostics of many diseases. One of the most common tests recommended by the physicians is complete blood cell count (CBC). In chemotherapy and radiation therapy, the blood cell production needs to be monitored. Inflammation, leukemia, tissue injury, bone marrow failure and immunodeficiency can be identified by the irregular WBC counts and their differentials. Thus, a microfluidic, disposable, economical CBC would help in monitoring all these diseases with more efficiency and care. Cell counting in general, and ability to count the specific cells, would greatly help in clinical diagnostics. Currently, flow cytometers and hematology analyzers are used for this purpose, but they have not been able to penetrate in the resource-limited settings around the world because of being expensive and because they require trained technicians to operate. Over many years, people have developed microfluidic devices for cell counting, which could provide a portable and economical solution to the problem of cell counting at point-of-care.
In this dissertation, we present a technique for counting the white blood cells and differentiating some of its sub-types within a microfluidic device. Starting with the whole blood, the red blood cells are lysed by saponin and formic acid. Quenching solution composed of phosphate buffer saline is infused in the device to halt the lysing process and maintain the pH of the solution. The remaining white blood cells are then passed across microfabricated electrodes within a microfluidic channel. The impedance is measured at 303 kHz and 1.7 MHz signals. Cells are captured in a capture chamber where the specific antibody is immobilized. The remaining cells are counted again and the difference in cell counts give the number of captured cells. We have counted CD4 and CD8 T cells from our biochip using healthy and HIV infected blood samples. The cell counts from the biochip are compared with those from the flow cytometer, showing the high correlation between the two techniques. We have also characterized the coincidence detection and show that cell counting with coincidence detection improves the accuracy of the counting results.
We have translated this technology to perform a complete blood cell (CBC) count from a drop of blood. The total leukocyte count and its 2-part differential (lymphocytes, monocytes + granulocytes) were obtained with the single counter. The blood sample is initially lysed, and then quenched, to get the total leukocyte count. At the entrance counter, lymphocytes can be electrically differentiated from monocytes+ neutrophils. We have used blood samples from healthy donors, patients admitted to the intensive care unit (ICU) and patients undergoing chemotherapy to get the complete dynamic range of cells. Erythrocytes and platelets have a very high concentration in the blood sample and require sample dilution for accurate cell counting. Platelet counting was more challenging using the same aperture counting channel because of the 1-2 μm diameter…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Boppart%2C%20Stephen%20A.%22%29&pagesize-30">Boppart, Stephen A. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Jain%2C%20Kanti%22%29&pagesize-30">Jain, Kanti (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Liu%2C%20Gang%20Logan%22%29&pagesize-30">Liu, Gang Logan (committee member).
Subjects/Keywords: Complete Blood Cell count; HIV/ AIDS Diagnosis; CD4, CD8 cell counting; Coincidence detection; Surface capture of cells; Sepsis Diagnosis; CD64 Expression
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hassan, U. (2015). A microfluidic biosensor to electrically enumerate blood cells at point-of-care for infectious disease diagnosis and management. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/78743
Chicago Manual of Style (16th Edition):
Hassan, Umer. “A microfluidic biosensor to electrically enumerate blood cells at point-of-care for infectious disease diagnosis and management.” 2015. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/78743.
MLA Handbook (7th Edition):
Hassan, Umer. “A microfluidic biosensor to electrically enumerate blood cells at point-of-care for infectious disease diagnosis and management.” 2015. Web. 17 Apr 2021.
Vancouver:
Hassan U. A microfluidic biosensor to electrically enumerate blood cells at point-of-care for infectious disease diagnosis and management. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2015. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/78743.
Council of Science Editors:
Hassan U. A microfluidic biosensor to electrically enumerate blood cells at point-of-care for infectious disease diagnosis and management. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/78743
University of Illinois – Urbana-Champaign
14.
Salm, Eric.
Transistor-based biosensing: expanding the functionality of field effect transistors.
Degree: PhD, 0408, 2014, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/49827
► Since the invention of Polymerase Chain Reaction (PCR)-based amplification of nucleic acids by Kary Mullis in 1983(1), researchers have spent significant efforts to improve the…
(more)
▼ Since the invention of Polymerase Chain Reaction (PCR)-based amplification of nucleic acids by Kary Mullis in 1983(1), researchers have spent significant efforts to improve the sensitivity and selectivity of PCR assay and have dramatically enhanced its application(2). PCR is now an integral tool of modern biotechnology processes and biological identification. Due to the growing demands for on-site, rapid diagnosis, attention has been paid in realizing portable, fast, and low cost PCR machines. Through expanding the uses of the field effect transistor platform to include a novel design of heating/cooling, ultra-localized cell lysis, and electrical detection of nucleic acid amplification using an on-chip electrode, this thesis aims to enable the next generation of portable biosensors for primary care and on-site diagnostics.
Chapter 2 presents an overview of the current state-of-the-art of electrical biosensors. In keeping with the goal of complete, integrated systems, methods for both sample preparation and detection were evaluated. Electrical sample preparation methods provide a unique opportunity to utilize electrical fields for cell lysis, concentration, and sample flow without the need for additional moving parts or reagents. Electrical detection methods offer a means of reducing reagents by eliminating the need for optical labels. Together, electrical sample preparation and detection will aid the development of portable, low-cost integrated biosensors.
Chapters 3 and 4 look at utilizing transistors as platform for sample preparation and manipulation through DNA denaturation and cell lysis. Through application of a 10MHz, AC field between the shorted source-drain and the back-gate of the chip, fringing electric fields located just above the transistor surface are generated. These fringing electric fields can be used for dielectric relaxation of water molecules to generate heat for DNA denaturation as discussed in chapter 3, or to generate a transmembrane potential across a cellular membrane for ultra-localized cell lysis as discussed in chapter 4. These methods expand the functionality of the transistor platform by extending their uses into the realm of sample manipulation.
Since the development of ISFETs for biosensing applications, transistors have been used for detection of biological analytes. Chapter 5 extends this functionality to electrical detection of nucleic acid amplification with an on-chip quasi-reference electrode. This method eliminates the need for a bulky/difficult to fabricate reference electrode and enables parallel detection of a large array of individual nucleic acid amplification reaction volumes. This method promises to reduce cost of PCR assays by eliminating optical components as well as improve the portability of the equipment by localizing the detection element to a disposable chip.
As discussed in the future outlook presented in chapter 6, the silicon CMOS compatible technologies introduced can be made portable, rapidly heat and cool reaction volumes, and remain…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Alam%2C%20Muhammad%20A.%22%29&pagesize-30">Alam, Muhammad A. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Cunningham%2C%20Brian%20T.%22%29&pagesize-30">Cunningham, Brian T. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Liu%2C%20Gang%20Logan%22%29&pagesize-30">Liu, Gang Logan (committee member).
Subjects/Keywords: Electrical biosensor; ion-sensitive field-effect transistor (ISFET); Field Effect Transistor (FET); reference field-effect transistor (REFET); solid-state electrode; nucleic acid amplification; Loop-mediated isothermal amplification (LAMP); Polymerase chain reaction (PCR); single cell lysis; droplet; DNA denaturation
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Salm, E. (2014). Transistor-based biosensing: expanding the functionality of field effect transistors. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/49827
Chicago Manual of Style (16th Edition):
Salm, Eric. “Transistor-based biosensing: expanding the functionality of field effect transistors.” 2014. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/49827.
MLA Handbook (7th Edition):
Salm, Eric. “Transistor-based biosensing: expanding the functionality of field effect transistors.” 2014. Web. 17 Apr 2021.
Vancouver:
Salm E. Transistor-based biosensing: expanding the functionality of field effect transistors. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2014. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/49827.
Council of Science Editors:
Salm E. Transistor-based biosensing: expanding the functionality of field effect transistors. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2014. Available from: http://hdl.handle.net/2142/49827
University of Illinois – Urbana-Champaign
15.
Watkins, Nicholas.
An electrical microcytometer for portable blood analysis in global health applications.
Degree: PhD, 1200, 2012, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/32048
► For three decades the AIDS (Acquired Immune Deficiency Syndrome) pandemic has killed millions and currently affects tens of millions around the world. It has especially…
(more)
▼ For three decades the AIDS (Acquired Immune Deficiency Syndrome) pandemic has killed millions and currently affects tens of millions around the world. It has especially crippled resource-poor regions in the world such as sub-Saharan Africa, which contains two-thirds of the world's people living with AIDS. Antiretroviral therapy (ART) to combat the human immunodeficiency virus (HIV) has become more accessible to patients in these regions over the past several years, and has shown to improve the quality of their lives. However, the lack of objective diagnostics—CD4+ T lymphocyte counts—to determine when to start ART and to monitor its success hinders the effective use of treatment in these regions. The industry standard of flow cytometry to obtain CD4+ T cell counts is too taxing on the debilitated healthcare infrastructure of undeveloped nations because of its high cost, technical demands, and lack of portability. Therefore, there is an urgent need to develop a portable, robust, and affordable point-of-care (PoC) CD4+ T cell counter that can reach all HIV/AIDS patients, regardless of geography or socio-economical situation.
This dissertation addresses this great need through investigation of a miniaturized, portable PoC platform that can provide CD4+ T cell counts in less than 15 minutes. Standard microfabrication techniques have been used to create a microfludic biochip, which uses electrical impedance sensing to analyze small sample volumes (~10 µL) of blood. The biochip progressively gains more functionality during the study. First, a chip was designed to confirm that the electrical impedance pulse counting technique was a viable method to enumerate CD4+ T cells. Three-dimensional hydrodynamic focusing was used to take advantage of the laminar flow regime found in microfludics and increase the counting accuracy of the device. Second, a differential counting stage was added that selectively counted CD4+ T cells from leukocyte (i.e., white blood cell) populations using immunoaffinity chromatography methods. Total leukocyte counts were obtained before and after the cells flowed through a CD4+ T cell depletion chamber. The difference between these two counts proved to be an accurate representation of the number of captured cells when verified with an optical control. Third, an erythrocyte (i.e., red blood cell) lysis module was added, with which to perform accurate counts on unprocessed blood samples, verified with flow cytometry controls. The success of this final stage suggests that the technology described is a viable answer to bringing PoC CD4+ T cell counts into resource-poor regions. An advantage of this label-free technology is that it can be readily expanded to diagnose other diseases and conditions simply by altering the specificity of its depletion chambers to other cell types.
Although this dissertation focuses on the specific application of PoC AIDS diagnostics, it also addresses the fundamentals in microfluidic cytometry. The impedance responses of cells under various conditions were studied,…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Cunningham%2C%20Brian%20T.%22%29&pagesize-30">Cunningham, Brian T. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Jain%2C%20Kanti%22%29&pagesize-30">Jain, Kanti (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Rodriguez%2C%20William%22%29&pagesize-30">Rodriguez, William (committee member).
Subjects/Keywords: Lab on a chip; point of care diagnostics; microfluidics; impedance cytometry; Acquired immunodeficiency syndrome (AIDS); Human immunodeficiency virus (HIV)
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APA (6th Edition):
Watkins, N. (2012). An electrical microcytometer for portable blood analysis in global health applications. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/32048
Chicago Manual of Style (16th Edition):
Watkins, Nicholas. “An electrical microcytometer for portable blood analysis in global health applications.” 2012. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/32048.
MLA Handbook (7th Edition):
Watkins, Nicholas. “An electrical microcytometer for portable blood analysis in global health applications.” 2012. Web. 17 Apr 2021.
Vancouver:
Watkins N. An electrical microcytometer for portable blood analysis in global health applications. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2012. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/32048.
Council of Science Editors:
Watkins N. An electrical microcytometer for portable blood analysis in global health applications. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2012. Available from: http://hdl.handle.net/2142/32048
University of Illinois – Urbana-Champaign
16.
Damhorst, Gregory L.
Microscale biosensors for HIV detection and viral load determination.
Degree: PhD, Bioengineering, 2015, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/89182
► The HIV/AIDS pandemic has killed 39 million people worldwide, and nearly as many people are living with HIV infection today. The global response to this…
(more)
▼ The HIV/AIDS pandemic has killed 39 million people worldwide, and nearly as many people are living with HIV infection today. The global response to this disease has come a long way since the emergence of HIV in the early 1980s, including more than 64 billion USD in international spending between 2002 and 2013 alone [1]. Due to the worldwide effort, HIV infection has been transformed from a death sentence into a manageable, chronic illness that can have limited impact on lifespan when treated properly [2]. Antiretroviral therapy, public health campaigns, and other education and prevention efforts have facilitated an age in which no one, regardless of age, gender, sexual orientation, nationality, or socioeconomic status should face despair on account of this infection. However, barriers persist to bringing proper care to millions of people worldwide, including access to testing and the diagnostic tools necessary for proper administration of therapy.
Following serological testing to establish HIV-positive status, the current standard of care requires monitoring of CD4+ T lymphocyte counts and plasma HIV viral load to guide administration of antiretroviral therapy. For many individuals living with HIV worldwide, the expensive and sophisticated laboratory instruments necessary for these measurements are extremely difficult to access due to poor healthcare infrastructure and lack of technical personnel. For those who are capable of bearing the expense and inconvenience of traveling to facilities that can provide one or both of these measurements, continuing care can be hindered by difficulties in patient follow-up. A point-of-care technology capable of performing these essential measurements to HIV therapy, therefore, is a critical need worldwide.
Here we explore solutions rooted in micro- and nanotechnology principles to address this immense challenge in global health. Point-of-care diagnostics which meet the following criteria could improve the way that HIV/AIDS is treated, particularly in remote and resource-limited settings: low-cost assays (approximately $10 or less), small sample volumes (approximately 10 μL or less), rapid measurements (approximately 10 minutes or less), as well as technologies that are easy to use and portable. Our expertise in this area began with the development of a lab-on-a-chip micro-cytometer for CD4+ T lymphocyte enumeration from a drop of whole blood, which was tested on HIV-positive patients in the
Champaign-
Urbana, IL area and matched results from clinical flow cytometry at Carle Foundation Hospital in
Urbana, IL [3]. This thesis describes work on the complementary measurement, viral load detection, aimed at meeting the ideal criteria described above for a point-of-care diagnostic technology.
Our approaches to viral load measurements follow two broad themes. First, we describe an antigen-based approach which leverages immuno-affinity recognition for whole virus particle detection. In this method, the novel component of our sensing system is an ion-filled liposome which, upon…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Cunningham%2C%20Brian%20T.%22%29&pagesize-30">Cunningham, Brian T. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Jokela%2C%20Janet%20A.%22%29&pagesize-30">Jokela, Janet A. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Pan%2C%20Dipanjan%22%29&pagesize-30">Pan, Dipanjan (committee member).
Subjects/Keywords: Viral load; Human immunodeficiency virus infection and acquired immune deficiency syndrome (HIV/AIDS); point-of-care; diagnostics; biosensors; human immunodeficiency virus (HIV); micro and nanotechnology; microfluidics
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APA (6th Edition):
Damhorst, G. L. (2015). Microscale biosensors for HIV detection and viral load determination. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/89182
Chicago Manual of Style (16th Edition):
Damhorst, Gregory L. “Microscale biosensors for HIV detection and viral load determination.” 2015. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/89182.
MLA Handbook (7th Edition):
Damhorst, Gregory L. “Microscale biosensors for HIV detection and viral load determination.” 2015. Web. 17 Apr 2021.
Vancouver:
Damhorst GL. Microscale biosensors for HIV detection and viral load determination. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2015. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/89182.
Council of Science Editors:
Damhorst GL. Microscale biosensors for HIV detection and viral load determination. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/89182
University of Illinois – Urbana-Champaign
17.
Duarte Guevara, Carlos.
Multiplexed label-free electrical detection of DNA amplification using field effect transistors.
Degree: PhD, Electrical & Computer Engr, 2016, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/90717
► The objective of this research project was to develop a miniaturized DNA amplification biosensor for the detection and identification of pathogenic bacteria. Using tailored loop-mediated…
(more)
▼ The objective of this research project was to develop a miniaturized DNA amplification biosensor for the detection and identification of pathogenic bacteria. Using tailored loop-mediated isothermal amplification (LAMP) and field effect transistors, we developed a microchip platform for multiplexed screening of samples querying the presence of multiple pathogenicity genes. In our platform, ion-sensitive field effect transistors (ISFETs) detect the incorporation of nucleotides during LAMP by monitoring changes in the solution's acidity. Employing transistors as biosensors enables label-free detection of the reaction, simple multiplexing, and seamless integration with required electronics for data acquisition. These characteristics of the detection system and protocols that we developed will make genotyping analysis simple and readily available for different applications that would benefit from low cost, portability, and ease-of-use. Here, we present a series of studies performed in three experimental setups that are related to the multiplexed electrical detection of LAMP and culminate in a large ISFET sensor array microchip that monitors DNA amplification reactions. A first chip consisted of 30 nL silicon oxide wells that were prepared with dried nucleic acid primers for multiplexed on-chip amplification. This initial study demonstrated the high specificity and low limit of detection of on-chip parallel LAMP when used for the detection of E.coli O157, S.enterica, L. monocytogenes, and non O157 Shiga-toxin producing E.coli of the `big six' group. Then, a second chip with novel individually addressable dual-gated ISFETs was fabricated in collaboration with Taiwan Semiconductor Manufacturing Company (TSMC). These devices were used to evaluate and optimize their pH sensing ability, develop methods to do label-free detection of LAMP, and study the sensor performance when biased with polypyrrole quasi-reference electrodes. The last platform, that demonstrates the impressive scalability of the semiconductor technology, is a chip with over a million ISFET sensors distributed in a 7x7 mm2 area. The use of on-chip decoding and routing circuits enables the parallel operation of 1024x1024 sensors in an array for massively multiplexed biosensing. In this platform we applied methods and systems developed previously to perform parallel electrical detection of foodborne pathogens by monitoring DNA amplification reactions in micro-chambers of 250 nL detecting down to 25 copies/reaction in less than 60 min. We demonstrate that the intrinsic redundancy of the high density ISFET array enabled clear identification of electrical signals resulting from the amplification reaction. This microchip for the detection of DNA and the related protocols on reaction miniaturization, parallelism, and electrical detection are poised to be the basis of new detection systems that bring the impressive advances of the semiconductor industry into biological applications.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Cunningham%2C%20Brian%22%29&pagesize-30">Cunningham, Brian (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Liu%2C%20Logan%22%29&pagesize-30">Liu, Logan (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Adesida%2C%20Ilesanmi%22%29&pagesize-30">Adesida, Ilesanmi (committee member).
Subjects/Keywords: Field effect transistors (FET) biosensor; Complementary metal-oxide semiconductor (CMOS)-compatible; Ion-sensitive field effect transistors (ISFET); Loop-mediated isothermal amplification; Food safety
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APA ·
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MLA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Duarte Guevara, C. (2016). Multiplexed label-free electrical detection of DNA amplification using field effect transistors. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/90717
Chicago Manual of Style (16th Edition):
Duarte Guevara, Carlos. “Multiplexed label-free electrical detection of DNA amplification using field effect transistors.” 2016. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/90717.
MLA Handbook (7th Edition):
Duarte Guevara, Carlos. “Multiplexed label-free electrical detection of DNA amplification using field effect transistors.” 2016. Web. 17 Apr 2021.
Vancouver:
Duarte Guevara C. Multiplexed label-free electrical detection of DNA amplification using field effect transistors. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2016. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/90717.
Council of Science Editors:
Duarte Guevara C. Multiplexed label-free electrical detection of DNA amplification using field effect transistors. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2016. Available from: http://hdl.handle.net/2142/90717
University of Illinois – Urbana-Champaign
18.
Raman, Ritu.
3D printed muscle-powered bio-bots.
Degree: PhD, Mechanical Engineering, 2016, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/100415
► Complex biological systems sense, process, and respond to a range of environmental signals in real-time. The ability of such systems to adapt their functional response…
(more)
▼ Complex biological systems sense, process, and respond to a range of environmental signals in real-time. The ability of such systems to adapt their functional response to dynamic external signals motivates the use of biological materials in other engineering applications. Recent advances in 3D printing have enabled the manufacture of complex structures from biological materials. We have developed a projection stereolithographic 3D printing apparatus capable of patterning cells and biocompatible polymers at physiologically relevant length scales, on the order of single cells. This enables reverse engineering in vitro model systems that recreate the structure and function of native tissue for applications ranging from high-throughput drug testing to regenerative medicine.
While reverse engineering native tissues and organs has important implications in biomedical engineering, the ability to “build with biology” presents the next generation of engineers with both a unique design challenge and opportunity. Specifically, we now have the ability to forward engineer bio-hybrid machines and robots (bio-bots) that harness the adaptive functionalities of biological materials to achieve more complex functional behaviors than machines composed of synthetic materials alone. Perhaps the most intuitive demonstration of a “living machine” is a system that can generate force and produce motion. To that end, we have designed and 3D printed locomotive bio-bots, powered by external electrical and optical stimuli. In addition to being the first demonstrations of untethered locomotion in skeletal musclepowered soft robots, these bio-hybrid machines have served as meso-scale models for studying tissue self-assembly, maturation, damage, remodeling, and healing in vitro.
Bio-hybrid machines that can dynamically sense and adaptively respond to a range of environmental signals have broad applicability in healthcare applications such as dynamic implants or targeted drug delivery. Advanced research in exoskeletons and hyper-natural functionality could even extend the useful application of such machines to national defense and environmental cleanup. We have developed a modular skeletal muscle bioactuator that can serve as a fundamental building block for such machines, setting the stage for future generations of bio-hybrid machines that can self-assemble, self-heal, and perhaps even self-replicate to target grand engineering challenges. Furthermore, we present a robust optimized protocol for manufacturing 3D printed muscle-powered biological machines, and a mechanism to incorporate biological “building blocks” into the toolbox of the next generation of engineers and scientists.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Saif%2C%20Taher%22%29&pagesize-30">Saif, Taher (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Kong%2C%20Hyunjoon%22%29&pagesize-30">Kong, Hyunjoon (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Popescu%2C%20Gabriel%22%29&pagesize-30">Popescu, Gabriel (committee member).
Subjects/Keywords: 3D printing; tissue engineering; optogenetics; bioactuators; soft robotics
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APA ·
Chicago ·
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Export
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APA (6th Edition):
Raman, R. (2016). 3D printed muscle-powered bio-bots. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/100415
Chicago Manual of Style (16th Edition):
Raman, Ritu. “3D printed muscle-powered bio-bots.” 2016. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/100415.
MLA Handbook (7th Edition):
Raman, Ritu. “3D printed muscle-powered bio-bots.” 2016. Web. 17 Apr 2021.
Vancouver:
Raman R. 3D printed muscle-powered bio-bots. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2016. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/100415.
Council of Science Editors:
Raman R. 3D printed muscle-powered bio-bots. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2016. Available from: http://hdl.handle.net/2142/100415
University of Illinois – Urbana-Champaign
19.
Corbin, Elise.
Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors.
Degree: PhD, 0133, 2014, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/46832
► Cancer is an intricate disease that stems from a number of different mutations in a cell. These mutations often control the cellular growth and proliferation,…
(more)
▼ Cancer is an intricate disease that stems from a number of different mutations in a cell. These mutations often control the cellular growth and proliferation, a hallmark of cancer, and give rise to many altered biophysical properties. There exists a complex relationship between the behavior of a cell, its physical properties, and its surrounding environment. Knowledge gleaned from cellular biomechanics can lead to an improved understanding of disease progression and provide methods to target it. There are many studies that look at biophysical changes on a large population level, though there is much information that is lost by treating populations as homogeneous in properties and cell cycle phase. Biophysical studies on individual cells can link mechanics with function through coordination with the cell cycle, which is a fundamental physiological process that is crucial for understanding cellular physiology and metabolism. Development of more precise, reliable, and versatile measurement techniques will provide a greater understanding the physical properties of a cell and how they affect its behavior. Microelectromechanical systems (MEMS) technology can provide tools for manipulating, processing, and analyzing single cells, thus enabling detailed analyses of their biophysical properties.
Growth is a vital element of the cell cycle, and cell mass homeostasis ensures that the cell mass and cell cycle transitions are coordinately linked. An accurate measurement of growth throughout the cell cycle is fundamental to understanding mechanisms of cellular proliferation in cancer. Growth can be identified through many ways; however, cell mass has been unexplored until the recent development of cantilever-type MEMS devices for mass sensing through resonant frequency shift. Measuring the dependency of growth rate on cellular mass may help explain the coordination and regulation of the cell cycle. However, MEMS mass sensing devices still require further development and characterization in order to reliably investigate long-term cell growth over the duration of the cell cycle.
This dissertation focuses on the use of MEMS resonant pedestal sensors for measuring the mass and growth rate of single cancer cells. This work included characterization and improvement of the sensors to address current challenges in the measurement of long-term growth rate. The MEMS resonant pedestal sensors were first used to measure physical properties of biomaterials, including the micromechanical properties of hydrogels through verification of stiffness effect on mass measurements. Before studying live cells, modifications to the fabrication process were introduced to improve cell capture and retention. These include integration of an on-chip microfluidic system for delivery of fluids during mass measurements and the micro-patterning of sensor surfaces for select functionalization and passivation. These modifications enable long-term measurement of the changes in mass of normal and cancerous cells over time. This is the first investigation of the…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">
Bashir,
Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22King%2C%20William%20P.%22%29&pagesize-30">King, William P. (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Wagoner%20Johnson%2C%20Amy%20J.%22%29&pagesize-30">Wagoner Johnson, Amy J. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Prasanth%2C%20Supriya%20G.%22%29&pagesize-30">Prasanth, Supriya G. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Kong%2C%20Hyun%20Joon%22%29&pagesize-30">Kong, Hyun Joon (committee member).
Subjects/Keywords: Micromechanical Sensors; Breast Cancer; Cell Mass; Cell Growth Rate; Cell Stiffness; Micro-Patterning; Long-Term Growth
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APA ·
Chicago ·
MLA ·
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Manager
APA (6th Edition):
Corbin, E. (2014). Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/46832
Chicago Manual of Style (16th Edition):
Corbin, Elise. “Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors.” 2014. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/46832.
MLA Handbook (7th Edition):
Corbin, Elise. “Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors.” 2014. Web. 17 Apr 2021.
Vancouver:
Corbin E. Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2014. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/46832.
Council of Science Editors:
Corbin E. Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2014. Available from: http://hdl.handle.net/2142/46832
University of Illinois – Urbana-Champaign
20.
Won, Sang M.
Flexible, stretchable, and transient electronics for integration with the human body.
Degree: PhD, Electrical & Computer Engr, 2019, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/105584
► Technologies capable of establishing intimate, long-lived interfaces to the human body have broad utility in continuous measurement of physiological status, with the potential to significantly…
(more)
▼ Technologies capable of establishing intimate, long-lived interfaces to the human body have broad utility in continuous measurement of physiological status, with the potential to significantly lower tissue injury and irritation after implants. The development of such soft, biocompatible platforms and integrating them into a biotissue-interfaced system requires suitable choice of materials and engineered structures. Specific directions include overall miniaturization (e.g., Si nanomembrane) or composite material structure (e.g., carbon black doped elastomer) that provide effective mechanics to match those of biological tissues. This dissertation presents combined experimental and theoretical investigations of such functional systems that offer flexibility and stretchability, while maintaining operational performance and mechanical robustness. The dissertation begins with a fundamental study of responsive monocrystalline silicon nanomembrane as a flexible electromechanical sensor element. Subsequent chapters highlight integration with active components for wireless addressing, multiplexing, and local amplification, with multimodal operation in a thin, soft, skin-like platform. The resulting biointegrated system enables (1) sensitive health monitoring system, (2) multifunctional tactile sensor, (3) high-density neural interfaces, and (4) physically transient, implantable electronics, all with the capability of stable operation for long timeframes.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Rogers%2C%20John%20A%22%29&pagesize-30">Rogers, John A (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Rogers%2C%20John%20A%22%29&pagesize-30">Rogers, John A (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Li%2C%20Xiuling%22%29&pagesize-30">Li, Xiuling (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Cunningham%2C%20Brian%20T%22%29&pagesize-30">Cunningham, Brian T (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Lyding%2C%20Joseph%20W%22%29&pagesize-30">Lyding, Joseph W (committee member).
Subjects/Keywords: FLEXIBLE ELECTRONICS; STRETCHABLE ELECTRONICS; IMPLANTABLE ELECTRONICS
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APA ·
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MLA ·
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Export
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APA (6th Edition):
Won, S. M. (2019). Flexible, stretchable, and transient electronics for integration with the human body. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/105584
Chicago Manual of Style (16th Edition):
Won, Sang M. “Flexible, stretchable, and transient electronics for integration with the human body.” 2019. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/105584.
MLA Handbook (7th Edition):
Won, Sang M. “Flexible, stretchable, and transient electronics for integration with the human body.” 2019. Web. 17 Apr 2021.
Vancouver:
Won SM. Flexible, stretchable, and transient electronics for integration with the human body. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2019. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/105584.
Council of Science Editors:
Won SM. Flexible, stretchable, and transient electronics for integration with the human body. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2019. Available from: http://hdl.handle.net/2142/105584
University of Illinois – Urbana-Champaign
21.
Long, Kenneth D.
Development of spectroscopic smartphone biosensors for point-of-care applications.
Degree: PhD, Bioengineering, 2018, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/101252
► This dissertation explores the combination of two emergent areas within contemporary biosensing, smartphone based spectroscopy and photonic crystal enhanced microscopy, and how these technologies can…
(more)
▼ This dissertation explores the combination of two emergent areas within contemporary biosensing, smartphone based spectroscopy and photonic crystal enhanced microscopy, and how these technologies can be combined to produce a fundamentally novel point-of-care testing paradigm: a portable device platform capable of non-amplifying, digital-detection for high-sensitivity diagnostics. In this work, I describe the development of this system, moving from usage-specific benchtop and smartphone based devices demonstrating proof-of-concept capabilities, to a multimodal smartphone platform compatible with thousands of existing spectroscopic assays. The resulting smartphone biosensor can perform various clinically-relevant tests with physiologically-relevant sensitivities. Next, photonic crystal enhanced microscopy is described for uses in the micrometer and nanometer scales for use both to study cellular and subcellular behavior and also to perform single-particle attachment quantification. Finally, this work explores how the single-particle attachment quantification capability can be leveraged to measure HIV viral load using a novel biosensor, designed specifically developed for a smartphone based platform for point of care applications.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Cunningham%2C%20Brian%20T.%22%29&pagesize-30">Cunningham, Brian T. (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Cunningham%2C%20Brian%20T.%22%29&pagesize-30">Cunningham, Brian T. (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Boppart%2C%20Stephen%20A.%22%29&pagesize-30">Boppart, Stephen A. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Achenbach%2C%20Chad%20J.%22%29&pagesize-30">Achenbach, Chad J. (committee member).
Subjects/Keywords: Smartphone biosensing; point-of-care testing; photonic crystal; photonic crystal enhanced microscopy; digital detection; smartphone spectroscopy; HIV viral load; smartphone diagnostics; POCT; PCEM; single nanoparticle detection; TRI-Analyzer; Spectral TRI-Analyzer
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APA (6th Edition):
Long, K. D. (2018). Development of spectroscopic smartphone biosensors for point-of-care applications. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/101252
Chicago Manual of Style (16th Edition):
Long, Kenneth D. “Development of spectroscopic smartphone biosensors for point-of-care applications.” 2018. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/101252.
MLA Handbook (7th Edition):
Long, Kenneth D. “Development of spectroscopic smartphone biosensors for point-of-care applications.” 2018. Web. 17 Apr 2021.
Vancouver:
Long KD. Development of spectroscopic smartphone biosensors for point-of-care applications. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/101252.
Council of Science Editors:
Long KD. Development of spectroscopic smartphone biosensors for point-of-care applications. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/101252
University of Illinois – Urbana-Champaign
22.
Heiranian, Mohammad.
Molecule transport in nanopores with applications to water purification, power generation and disease diagnosis.
Degree: PhD, Theoretical & Applied Mechans, 2020, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/108510
► High performance water transport in nanopores has drawn a great deal of attention in a variety of applications, such as water desalination, power generation and…
(more)
▼ High performance water transport in nanopores has drawn a great deal of attention in a variety of applications, such as water desalination, power generation and biosensing. A single-layer MoS2 nanopore is shown, here, to possess high water transport rate and strong salt rejection rate making it ideal for water desilation. High water transport enhancement factors in carbon-based nanopores have been reported over the classical Hagen-Poiseuille (HP) equation which does not account for the physics of transport at molecular scale. Instead, comparing the experimentally measured transport rates to that of a theory, that accounts for the microscopic physics of transport, would result in enhancement factors approaching unity. Here, molecular corrections are introduced into HP equation by considering the variation of key hydrodynamical properties (viscosity and friction) with thickness and diameter of pores in ultrathin graphene and finite-length carbon nanotubes (CNTs) using Green-Kubo relations and molecular dynamics (MD) simulations. The corrected HP (CHP) theory, successfully predicts the permeation rates from non-equilibrium MD pressure driven flows. The previously reported enhancement factors over no-slip HP (of the order of 1000) approach unity when the permeations are normalized by the CHP flow rates.
In a follow-up study, we revisit Sampson’s theory after more than a century to account for the surface chemistry of nanopores by incorporating slippage and interfacial viscosity variation into the original Sampson’s theory. The HP theory works for flow in infinitely long tubes where end effects are neglected. In 1891, Ralph Allen Sampson came up with a formula, known as Sampson formula, within the fluid mechanics framework to describe flow in an infinitesimally thin orifice. Zeev Dagan, Sheldon Weinbaum and Robert Pfeffer published an article in the Journal of Fluid Mechanics in 1982, where the HP and Sampson formulas were combined to successfully describe flow in circular tubes of finite length. Although the Sampson formula is a powerful theory for end effects, it has been shown to lack accuracy for relatively small-radius pores (e.g., nanopores in single-layer graphene membranes) since it does not account for the molecular interface chemistry. We show that the corrected Sampson’s theory is able to accurately describe flow in ultrathin nanopores when compared to the data from molecular dynamics simulations. Combining our corrected Sampson formula with the HP equation, we can remarkably predict flow in not only ultrathin pores but also finite-length pores such as carbon nanotubes.
We also explored the structure and dynamics of aqueous ions in nanopores. At the nanopore interfaces, properties of ions are shown to differ largely from those of predicted by the classical ionic layering models (e.g., Gouy-Chapman electric double layer (EDL)) when the thickness of the nanopore is scaled down to the limit of ultrathin membranes (e.g, single-layer graphene). Here, using extensive molecular dynamics, the structure and dynamics…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Aluru%2C%20Narayana%22%29&pagesize-30">Aluru, Narayana (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Aluru%2C%20Narayana%22%29&pagesize-30">Aluru, Narayana (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Nam%2C%20SungWoo%22%29&pagesize-30">Nam, SungWoo (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Shukla%2C%20Diwakar%22%29&pagesize-30">Shukla, Diwakar (committee member).
Subjects/Keywords: Nanopores; Nanofluidics; biophysics; DNA sequencing; Desalination; Graphene; Carbon Nanotubes; Molybdenum disulfide; Electric double layers; Hydrodynamics
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APA (6th Edition):
Heiranian, M. (2020). Molecule transport in nanopores with applications to water purification, power generation and disease diagnosis. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/108510
Chicago Manual of Style (16th Edition):
Heiranian, Mohammad. “Molecule transport in nanopores with applications to water purification, power generation and disease diagnosis.” 2020. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/108510.
MLA Handbook (7th Edition):
Heiranian, Mohammad. “Molecule transport in nanopores with applications to water purification, power generation and disease diagnosis.” 2020. Web. 17 Apr 2021.
Vancouver:
Heiranian M. Molecule transport in nanopores with applications to water purification, power generation and disease diagnosis. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2020. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/108510.
Council of Science Editors:
Heiranian M. Molecule transport in nanopores with applications to water purification, power generation and disease diagnosis. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2020. Available from: http://hdl.handle.net/2142/108510
University of Illinois – Urbana-Champaign
23.
Estrada, David.
Reliability, power dissipation, sensing, and thermal transport in carbon nanomaterials and devices.
Degree: PhD, 1200, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/44405
► Energy consumption is a significant challenge across the globe ranging from power consumption in large-scale buildings to nanoscale devices. A fundamental examination of energy dissipation…
(more)
▼ Energy consumption is a significant challenge across the globe ranging from power consumption in large-scale buildings to nanoscale devices. A fundamental examination of energy dissipation in such contexts can lead to orders of magnitude improvements in energy efficiency. Emerging classes of nanomaterials, such as carbon nanotubes and 2-dimensional crystals (e.g. graphene), have presented new opportunities to improve energy use at the macro and nanoscale. However, much work remains to be done to fully understand the high-field reliability and fundamental properties of these nanomaterials in order to promote their widespread use in energy applications.
In this work, we investigate the reliability of carbon nanotube transistors by developing a pulsed measurement technique to suppress hysteresis for carbon nanotube (CNT) mobility measurements in air, in vacuum, and over a wide (80 – 453 K) temperature range. The use of this pulsed measurement technique provides a route towards measuring the device mobility without the effects of charge screening as well as the interface quality of low-dimensional systems and their surrounding bulk environments. We then use infrared thermometry to investigate power dissipation in carbon nanotube network (CNN) transistors and find the formation of distinct hot spots during operation. However, the average CNN temperature at breakdown is significantly lower than expected from the breakdown of individual nanotubes, which we attribute to extremely high regions of power dissipation at the nanotube junctions.
We then turn our attention to the fundamental properties of large-scale polycrystalline graphene films grown by chemical vapor deposition (CVD). We elucidate the chemical sensing mechanisms of such films, and find that linear defects or continuous lines of point defects are needed to enhance the chemical sensitivity of graphene. Therefore, simple chemiresistors made from CVD polycrystalline graphene could be used as highly sensitive pollutant detectors in “smart” climate control systems to reduce energy consumption by residential and commercial buildings. Lastly, we develop an electrical thermometry platform to investigate the practical tuning of thermal transport in layer-by-layer assembled graphene van der Waals (vdW) solids. We find thermal transport in a single layer of transferred CVD graphene is limited by substrate phonon and grain boundary scattering, but can be significantly enhanced by transferring subsequent layers of CVD graphene.
Overall, the research summarized in this dissertation represents a significant advancement in the understanding of the reliability and fundamental physical properties of emerging nanomaterials, which are increasingly finding their way to commercial applications.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Pop%2C%20Eric%22%29&pagesize-30">Pop, Eric (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Pop%2C%20Eric%22%29&pagesize-30">Pop, Eric (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Lyding%2C%20Joseph%20W.%22%29&pagesize-30">Lyding, Joseph W. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member),
Champaign%22%20%2Bcontributor%3A%28%22King%2C%20William%20P.%22%29&pagesize-30">King, William P. (committee member).
Subjects/Keywords: Carbon Nanotubes; Graphene; Hysteresis; Thermal Transport; Power Dissipation; Chemical Sensors
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APA ·
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APA (6th Edition):
Estrada, D. (2013). Reliability, power dissipation, sensing, and thermal transport in carbon nanomaterials and devices. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/44405
Chicago Manual of Style (16th Edition):
Estrada, David. “Reliability, power dissipation, sensing, and thermal transport in carbon nanomaterials and devices.” 2013. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/44405.
MLA Handbook (7th Edition):
Estrada, David. “Reliability, power dissipation, sensing, and thermal transport in carbon nanomaterials and devices.” 2013. Web. 17 Apr 2021.
Vancouver:
Estrada D. Reliability, power dissipation, sensing, and thermal transport in carbon nanomaterials and devices. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/44405.
Council of Science Editors:
Estrada D. Reliability, power dissipation, sensing, and thermal transport in carbon nanomaterials and devices. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/44405
24.
Suk, Myung eun.
Structure, property and transport mechanism of water and electrolytes in graphene nanopores.
Degree: PhD, 0133, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/45641
► Advances in fabrication, measurement and characterization have led to intense research in the area of nanoporous membranes. Owing to its ultrathin thickness, graphene nanopores are…
(more)
▼ Advances in fabrication, measurement and characterization have led to intense research in the area of nanoporous membranes. Owing to its ultrathin thickness, graphene nanopores are promising candidates for various applications such as water desalination, single molecule sensing, etc. Understanding water and ion transport mechanisms and properties in membranes is essential to characterize and design membranes for various applications mentioned above. In nanopores, transport mechanisms can differ from the continuum theory and transport properties under confinement can be different from the bulk values. In such a situation, molecular dynamics simulation is a useful tool to observe molecular level details. In this thesis, structure, properties and transport mechanism of water and ions in graphene nanopores are investigated in detail to realize the full potential of graphene nanopores, using molecular dynamics simulations.
Due to the unique water structure, confined in the radial direction and layered in the axial direction of the pore, water viscosity and slip length increase with a decrease in the pore radius, in contrast to water confined in a carbon nanotube. Due to the nanometer dimension of the pore, Reynolds number for pressure-driven water flow through graphene nanopores is very small and a linear relation between flow rate and applied pressure drop is observed. Hydrodynamic membrane length is introduced to effectively capture entrance and exit pressure losses. As the diameter of the pore increases, the water transport mechanism transitions from collective diffusion to frictional flow described by the modified Hagen–Poiseuille equation. Graphene membrane is shown to be ultra-efficient by comparing the permeation coefficient of graphene membrane to that of advanced membranes.
Water transport through graphene is compared with water transport through thin carbon nanotube (CNT) membranes. For smaller diameter membranes, where single-file structure is observed, water flux is lower through the graphene membrane compared to that of the CNT membrane, primarily due to the frequent rupture of hydrogen bonding network and L/D defect-like water orientation in the graphene pore. For larger diameter pores, where the water structure is not single-file, graphene membranes provide higher water flux compared to CNT membranes. Furthermore, in thin CNT membranes, the water flux did not vary significantly with the thickness of the membrane. This result is explained by the pressure distribution and plug-like velocity distribution in the CNT.
Finally, the static and dynamic properties of ions are investigated with and without an external electric field. Ion concentration in graphene nanopores sharply drops from the bulk concentration when the pore radius is smaller than 0.9 nm. Ion mobility in the pore is also smaller than bulk ion mobility due to the layered liquid structure in the pore-axial direction. The results show that a continuum analysis can be appropriate when pore radius is larger than 0.9 nm if pore conductivity is…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Aluru%2C%20Narayana%20R.%22%29&pagesize-30">Aluru, Narayana R. (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Aluru%2C%20Narayana%20R.%22%29&pagesize-30">Aluru, Narayana R. (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Jakobsson%2C%20Eric%22%29&pagesize-30">Jakobsson, Eric (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Saintillan%2C%20David%22%29&pagesize-30">Saintillan, David (committee member).
Subjects/Keywords: Nanofluidics; Molecular dynamics simulation; Membrane transport
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Suk, M. e. (2013). Structure, property and transport mechanism of water and electrolytes in graphene nanopores. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/45641
Chicago Manual of Style (16th Edition):
Suk, Myung eun. “Structure, property and transport mechanism of water and electrolytes in graphene nanopores.” 2013. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/45641.
MLA Handbook (7th Edition):
Suk, Myung eun. “Structure, property and transport mechanism of water and electrolytes in graphene nanopores.” 2013. Web. 17 Apr 2021.
Vancouver:
Suk Me. Structure, property and transport mechanism of water and electrolytes in graphene nanopores. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/45641.
Council of Science Editors:
Suk Me. Structure, property and transport mechanism of water and electrolytes in graphene nanopores. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/45641
University of Illinois – Urbana-Champaign
25.
Mir, Mustafa.
Quantitative phase imaging for cellular biology.
Degree: PhD, 1200, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/45668
► Measuring cellular level phenomena is challenging because of the transparent nature of cells and tissues, the multiple temporal and spatial scales involved, and the need…
(more)
▼ Measuring cellular level phenomena is challenging because of the transparent nature of cells and tissues, the multiple temporal and spatial scales involved, and the need for both high sensitivity (to single cell density, morphology, motility, etc.) and the ability to measure a large number of cells. Quantitative phase imaging (QPI) is an emerging field that addresses this need. New quantitative phase imaging modalities have emerged that provide highly sensitive information on cellular growth, motility, dynamics and spatial organization. These parameters can be measured from the sub-micron to millimeter scales and timescales ranging from milliseconds to days. In this thesis I discuss the development and use of QPI tools and analysis methods to explore several applications in both clinical and research settings. Through these applications I demonstrate that the quantitative information provided by QPI methods allows for analyzing biological systems in an unprecedented manner, creating opportunities to answer longstanding questions in biological sciences, and also enabling the study of phenomena that were previously inaccessible. Here I show results on blood cell analysis, single cell growth, cellular proliferation assays and neural network formation. These results prove that QPI provides unique and important insight into the behavior of biological systems and can be utilized to help address important needs in clinical settings as well as answer fundamental biological questions.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Popescu%2C%20Gabriel%22%29&pagesize-30">Popescu, Gabriel (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Popescu%2C%20Gabriel%22%29&pagesize-30">Popescu, Gabriel (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Prasanth%2C%20Supriya%20G.%22%29&pagesize-30">Prasanth, Supriya G. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Boppart%2C%20Stephen%20A.%22%29&pagesize-30">Boppart, Stephen A. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member).
Subjects/Keywords: Quantitative phase imaging; Spatial light interference microscopy; diffraction phase microscopy; red blood cell cytometry; cell growth; cycle dependent growth; neuronal network organization; cell proliferation; tomography; sub-cellular tomography; image analysis; interferometry; microscopy
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Mir, M. (2013). Quantitative phase imaging for cellular biology. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/45668
Chicago Manual of Style (16th Edition):
Mir, Mustafa. “Quantitative phase imaging for cellular biology.” 2013. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/45668.
MLA Handbook (7th Edition):
Mir, Mustafa. “Quantitative phase imaging for cellular biology.” 2013. Web. 17 Apr 2021.
Vancouver:
Mir M. Quantitative phase imaging for cellular biology. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/45668.
Council of Science Editors:
Mir M. Quantitative phase imaging for cellular biology. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/45668
University of Illinois – Urbana-Champaign
26.
Girdhar, Anuj.
Quantum transport in graphene nanotransistors.
Degree: PhD, Physics, 2015, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/78634
► Over the past decade, interest in using graphene in condensed-matter physics and materials science applications has exploded, owing to its unique electrical properties. Narrow strips…
(more)
▼ Over the past decade, interest in using graphene in condensed-matter physics and materials science applications has exploded, owing to its unique electrical properties. Narrow strips of graphene, called graphene nanoribbons, also display exotic behavior. A nanoribbon’s edge geometry determines its electronic transport properties, and the rich behavior
of conductance of nanoribbons in response to external potentials makes them ideal for use within transistors.
In this thesis, we work towards creating an accurate model of graphene nanoribbon transistors, and we asses two possible applications which exploit their amazing potential. We begin by outlining the basic theoretical and computational framework for the model developed in this work. We then demonstrate the capability of graphene nanoribbon transistors, with nanopores, to electronically detect, characterize, and manipulate translocating DNA
strands. Specifically, we explore the tunability of such devices, by examining the role of lattice geometry, such as a quantum point contact constriction, on their performance. We perform a demonstration of the ability to detect the passage of double and single-stranded
DNA, through molecular dynamics simulations. The transistors presented are capable of sensing the helical shape of double-stranded DNA molecules, the unraveling of a DNA helix into a planar-zipper form, and the passage of individual nucleotides of a single strand of DNA
through the nanopore. We outline a preliminary analysis on the proper design of a multilayer transistor stack to control both the electronic properties of the conducting membrane, as well as the motion of the DNA. Lastly, we present another type of nanoribbon device,
an all-carbon spintronic transistor for use in cascaded logic circuits. A thorough analysis of the transport properties of zigzag nanoribbon transistors in magnetic fields, in addition to the design and construction of logic gate circuits containing these spintronic transistors, is presented.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Leburton%2C%20Jean-Pierre%22%29&pagesize-30">Leburton, Jean-Pierre (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Mason%2C%20Nadya%22%29&pagesize-30">Mason, Nadya (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Schulten%2C%20Klaus%20J.%22%29&pagesize-30">Schulten, Klaus J. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member).
Subjects/Keywords: graphene; quantum; transport; nanoribbon; Deoxyribonucleic Acid (DNA); sequencing; transistor; nanopore; sensing; genome; monolayer; gate
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APA ·
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MLA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Girdhar, A. (2015). Quantum transport in graphene nanotransistors. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/78634
Chicago Manual of Style (16th Edition):
Girdhar, Anuj. “Quantum transport in graphene nanotransistors.” 2015. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/78634.
MLA Handbook (7th Edition):
Girdhar, Anuj. “Quantum transport in graphene nanotransistors.” 2015. Web. 17 Apr 2021.
Vancouver:
Girdhar A. Quantum transport in graphene nanotransistors. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2015. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/78634.
Council of Science Editors:
Girdhar A. Quantum transport in graphene nanotransistors. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/78634
University of Illinois – Urbana-Champaign
27.
Melhem, Molly Rami.
The use of biomaterials for stem cell therapies to prevent myocardial damage post-infarct.
Degree: PhD, Bioengineering, 2015, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/78766
► This thesis employed a stem cell encapsulating hydrogel patch to increase the amount of beneficial soluble factors that are delivered to the surface of damaged…
(more)
▼ This thesis employed a stem cell encapsulating hydrogel patch to increase the amount of beneficial soluble factors that are delivered to the surface of damaged heart tissue following a myocardial infarction. While current medical practices to address the immediate aftermath of a myocardial infarction (MI) have evolved tremendously, there are few, if any, techniques currently administered to slow, cease, or reverse the negative side effects of an occluded artery, such as the replacement of functional myocardium with non-contractile scar tissue. Because of this scar formation, survival of the initial heart attack is commonly accompanied by a decrease in left ventricular functioning due to wall thinning and ventricular enlargement. As a result of the slow, or absent, ability of cardiomyocytes to divide and repopulate the infarcted area, the burden of heart function lies on the surrounding tissue; a load that exhausts the healthy tissue and decreases the quality of life of heart attack survivors.
Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic avenue for post-MI treatment, in part due to the “survival signals” that they secrete. Previous work has shown that by increasing the amount of “survival signals” that are introduced to the damaged myocardium, the extent of cardiomyocyte cell death, and subsequent scar formation, can be decreased. While the therapeutic effects of these factors have been documented, one difficulty lies in the ability to maintain a constant flux of secreted factors to the damaged site. This project hypothesized that through the encapsulation of stem cells within an engineered hydrogel construct, the hurdle of soluble factor delivery at the site of injury could be overcome. A constant flux of paracrine factors to the heart surface would allow for cell recruitment to the site(s) of damage, prevention of tissue degradation due to inhospitable environments, and promotion of neovascularizion for sustainable tissue regeneration.
Using both a chick chorioallantoic membrane assay and a mouse model of MI, the following aims determined: 1) the vascularization potential of an MSC encapsulated patch, 2) the ability to deliver hydrogels containing pro-survival signals to the heart post-MI, and 3) the ability of these factors to decrease scar formation and improve cardiac function following a heart attack. Knowledge gained from this project will provide the basis for designing materials and strategies for similar studies in larger animal models and eventually for human clinical trials. Successful delivery of the MSC encapsulating patch, and subsequent decrease in myocardial degradation, will greatly improve the quality of life scores of individuals who have suffered heart failure. This increase in quality of life will aid in post-MI mobility and decrease the need for more intensive health care following the initial heart attack; overall decreasing the burden a strained heart has on both patients and the healthcare system.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Schook%2C%20Lawrence%22%29&pagesize-30">Schook, Lawrence (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Schook%2C%20Lawrence%22%29&pagesize-30">Schook, Lawrence (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Kong%2C%20Hyun%20Joon%22%29&pagesize-30">Kong, Hyun Joon (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member),
Champaign%22%20%2Bcontributor%3A%28%22DiPietro%2C%20Luisa%22%29&pagesize-30">DiPietro, Luisa (committee member).
Subjects/Keywords: Stem cell therapy; myocardial infarctions; tissue engineering; biomaterials
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APA (6th Edition):
Melhem, M. R. (2015). The use of biomaterials for stem cell therapies to prevent myocardial damage post-infarct. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/78766
Chicago Manual of Style (16th Edition):
Melhem, Molly Rami. “The use of biomaterials for stem cell therapies to prevent myocardial damage post-infarct.” 2015. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/78766.
MLA Handbook (7th Edition):
Melhem, Molly Rami. “The use of biomaterials for stem cell therapies to prevent myocardial damage post-infarct.” 2015. Web. 17 Apr 2021.
Vancouver:
Melhem MR. The use of biomaterials for stem cell therapies to prevent myocardial damage post-infarct. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2015. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/78766.
Council of Science Editors:
Melhem MR. The use of biomaterials for stem cell therapies to prevent myocardial damage post-infarct. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/78766
University of Illinois – Urbana-Champaign
28.
Mahadik, Bhushan Prakash.
Hydrogel platform to investigate the coordinated impact of niche signals on hematopoietic stem cell fate.
Degree: PhD, 0300, 2014, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/50454
► Hematopoietic stem cells (HSCs) regulate the process of hematopoiesis, which is the formation and development of all the body’s blood and immune cells. The HSCs…
(more)
▼ Hematopoietic stem cells (HSCs) regulate the process of hematopoiesis, which is the formation and development of all the body’s blood and immune cells. The HSCs ability to either self-renew or differentiate in order to produce these populations is determined by a number of known as well as hypothesized regulators of HSC biology in specific microenvironments, termed as ‘niches’, in vivo. However, in order to explore their therapeutic potential, controlling HSC fate in vitro is important. Current culture approaches lack the capacity to incorporate combinations of instructive signals to guide desired HSC fate decisions. Furthermore, approaches to examine in detail signaling mechanisms involved in cross-talk between multiple signals are largely underdeveloped. In this thesis, I describe the development of a gradient hydrogel platform that enables co-culture of HSCs with multiple niche components, namely exogenous niche cells, extracellular matrix proteins, and matrix-tethered biomolecular signals. Gradients in microenvironmental signals are common across the marrow; these gradients are thought to play a critical role in HSC signaling and migration. The biomaterial fabrication platform described here enables creation and subsequent analysis of gradient environments in an attempt to understand the effects of niche components on HSC fate. Building upon the gradient platform, we will also discuss the development of an approach to functionalize the biomaterial with proteins that provide an additional degree of instructive signals to manipulate HSC response. Finally, using a diffusion-limited hydrogel network we alter the balance of niche-cell mediated paracrine and HSC-mediated autocrine signals as a novel approach to manipulate HSC response. Balancing these signals in an in vitro platform may eventually offer a path for improved control over HSC self-renewal versus lineage specification for a range of clinical applications. An ultimate goal is developing the capacity to engineer HSC fate in vitro, enabling better clinical therapies and providing insights into blood-related cancers and disorders.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Harley%2C%20Brendan%20A.%22%29&pagesize-30">Harley, Brendan A. (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Harley%2C%20Brendan%20A.%22%29&pagesize-30">Harley, Brendan A. (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Kenis%2C%20Paul%20J.A.%22%29&pagesize-30">Kenis, Paul J.A. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Kong%2C%20Hyun%20Joon%22%29&pagesize-30">Kong, Hyun Joon (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member).
Subjects/Keywords: Hematopoietic stem cell; Hydrogel: Biomaterial; gradient; microfluidic
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APA ·
Chicago ·
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APA (6th Edition):
Mahadik, B. P. (2014). Hydrogel platform to investigate the coordinated impact of niche signals on hematopoietic stem cell fate. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/50454
Chicago Manual of Style (16th Edition):
Mahadik, Bhushan Prakash. “Hydrogel platform to investigate the coordinated impact of niche signals on hematopoietic stem cell fate.” 2014. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/50454.
MLA Handbook (7th Edition):
Mahadik, Bhushan Prakash. “Hydrogel platform to investigate the coordinated impact of niche signals on hematopoietic stem cell fate.” 2014. Web. 17 Apr 2021.
Vancouver:
Mahadik BP. Hydrogel platform to investigate the coordinated impact of niche signals on hematopoietic stem cell fate. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2014. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/50454.
Council of Science Editors:
Mahadik BP. Hydrogel platform to investigate the coordinated impact of niche signals on hematopoietic stem cell fate. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2014. Available from: http://hdl.handle.net/2142/50454
University of Illinois – Urbana-Champaign
29.
Li, Huan.
Solid-liquid interactions in microscale structures and devices.
Degree: PhD, 0133, 2012, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/29422
► Liquid-solid interactions become important as dimensions approach mciro/nano-scale. This dissertation focuses on liquid-solid interactions in two distinct applications: capillary driven self-assembly of thin foils into…
(more)
▼ Liquid-solid interactions become important as dimensions approach mciro/nano-scale. This dissertation focuses on liquid-solid interactions in two distinct applications: capillary driven self-assembly of thin foils into 3D structures, and droplet wetting of hydrophobic micropatterned surfaces.
The phenomenon of self-assembly of complex structures is common in biological systems. Examples include self-assembly of proteins into macromolecular structures and self-assembly of lipid bilayer membranes. The principles governing this phenomenon have been applied to induce self-assembly of millimeter scale Si thin films into spherical and other 3D structures, which are then integrated into light-trapping photovoltaic (PV) devices. Motivated by this application, we present a generalized analytical study of the self-folding of thin plates into deterministic 3D shapes, through fluid-solid interactions, to be used as PV devices. This study consists of developing a model using beam theory, which incorporates the two competing components — a capillary force that promotes folding and the bending rigidity of the foil that resists folding into a 3D structure. Through an equivalence argument of thin foils of different geometry, an effective folding parameter, which uniquely characterizes the driving force for folding, has been identified. A criterion for spontaneous folding of an arbitrarily shaped 2D foil, based on the effective folding parameter, is thus established. Measurements from experiments using different materials and predictions from the model match well, validating the assumptions used in the analysis.
As an alternative to the mechanics model approach, the minimization of the total free energy is employed to investigate the interactions between a fluid droplet and a flexible thin film. A 2D energy functional is proposed, comprising the surface energy of the fluid, bending energy of the thin film and gravitational energy of the fluid. Through simulations with Surface Evolver, the shapes of the droplet and the thin film at equilibrium are obtained. A critical thin film length necessary for complete enclosure of the fluid droplet, and hence successful self-assembly into a PV device, is determined and compared with the experimental results and mechanics model predictions. The results from the modeling and energy approaches and the experiments are all consistent.
Superhydrophobic surfaces, which have unique properties including self-cleaning and water repelling are desired in many applications. One excellent example in nature is the lotus leaf. To fabricate these surfaces, well designed micro/nano- surface structures are often employed. In this research, we fabricate superhydrophobic micropatterned Polydimethylsiloxane (PDMS) surfaces composed of micropillars of various sizes and arrangements by means of soft lithography. Both anisotropic surfaces, consisting of parallel grooves and cylindrical pillars in rectangular lattices, and isotropic surfaces, consisting of cylindrical pillars in square and hexagonal lattices,…
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Hsia%2C%20K.%20Jimmy%22%29&pagesize-30">Hsia, K. Jimmy (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Hsia%2C%20K.%20Jimmy%22%29&pagesize-30">Hsia, K. Jimmy (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Saif%2C%20M.%20Taher%20A.%22%29&pagesize-30">Saif, M. Taher A. (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Hilgenfeldt%2C%20Sascha%22%29&pagesize-30">Hilgenfeldt, Sascha (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member).
Subjects/Keywords: Self-assembly; capillary force; folding; thin films; bending rigidity; hydrophobicity; wetting; contact line; contact angle; contact angle hysteresis; microtexture; Micropattern; force sensor; Pinning; depinning
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APA ·
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MLA ·
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CSE |
Export
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APA (6th Edition):
Li, H. (2012). Solid-liquid interactions in microscale structures and devices. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/29422
Chicago Manual of Style (16th Edition):
Li, Huan. “Solid-liquid interactions in microscale structures and devices.” 2012. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/29422.
MLA Handbook (7th Edition):
Li, Huan. “Solid-liquid interactions in microscale structures and devices.” 2012. Web. 17 Apr 2021.
Vancouver:
Li H. Solid-liquid interactions in microscale structures and devices. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2012. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/29422.
Council of Science Editors:
Li H. Solid-liquid interactions in microscale structures and devices. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2012. Available from: http://hdl.handle.net/2142/29422
University of Illinois – Urbana-Champaign
30.
Ansari, Ali.
Engineering and optimizing physiological cell isolation via the secondary anchor targeted cell release system.
Degree: PhD, Bioengineering, 2018, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/100979
► Cancer treatment regimens, such as chemotherapies are fundamentally limited through patient drug resistance, as patients respond differentially due to these individualized resistances and differences in…
(more)
▼ Cancer treatment regimens, such as chemotherapies are fundamentally limited through patient drug resistance, as patients respond differentially due to these individualized resistances and differences in biomarker expression on cells. Quantification of these biomarkers, then, would allow a methodology for designing personalized treatments and regimens that the patients would no longer be resistant to. However, techniques designed to purify or isolate cells to quantify these biomarkers are not designed to maintain physiological cell expression. In order to develop an isolation modality to preserve receptor numbers, I have developed and optimized the Secondary Anchor Targeted Cell Release (SATCR) system to separate out cells of interest for downstream analysis. The SATCR enables both capture and release of cells through the targeting of the secondary anchor- streptavidin- through the introduction of 4mM biotin into the system. The system has been optimized to preserve physiological wall shear stress, receptor quantity and cell diameter of cells isolated through the system. This allows for our system to create a more physiologically faithful modality for downstream analysis- potentially opening the door to more physiological analyses of purified cell samples for personalized medicine.
Surface functionalization allows for the customization and adaption of surfaces for a variety of needs and applications. We have used surface functionalization to adapt glass and PDMS surfaces with the SATCR surface, but there exists a great deal of mineable space for surface functionalization and its adoption in existing modalities. This space includes moving the SATCR surface from static glass based systems into dynamic microfluidic glass and PDMS systems, and possibly even further to non-standard functionalized materials such as polyvinyl chloride. The functionalization of alternate materials would allow further customization and easier adoption of the capture surface into other substrates, further increasing the utility and degrees of freedom for the SATCR capture surface. In addition to substrate alteration, further adaptions and modifications can advance and optimize the SATCR technology to enable more effective and selective isolation of cells through SATCR integration.
Advisors/Committee Members: Champaign%22%20%2Bcontributor%3A%28%22Imoukhuede%2C%20Princess%20I%22%29&pagesize-30">Imoukhuede, Princess I (advisor),
Champaign%22%20%2Bcontributor%3A%28%22Imoukhuede%2C%20Princess%20I%22%29&pagesize-30">Imoukhuede, Princess I (Committee Chair),
Champaign%22%20%2Bcontributor%3A%28%22Murphy%2C%20Catherine%20J%22%29&pagesize-30">Murphy, Catherine J (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bhargava%2C%20Rohit%22%29&pagesize-30">Bhargava, Rohit (committee member),
Champaign%22%20%2Bcontributor%3A%28%22Bashir%2C%20Rashid%22%29&pagesize-30">Bashir, Rashid (committee member).
Subjects/Keywords: Surface Functionalization; Microfluidics; Cell Isolation; Streptavidin; Biotin; Cell Patterning
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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APA (6th Edition):
Ansari, A. (2018). Engineering and optimizing physiological cell isolation via the secondary anchor targeted cell release system. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/100979
Chicago Manual of Style (16th Edition):
Ansari, Ali. “Engineering and optimizing physiological cell isolation via the secondary anchor targeted cell release system.” 2018. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 17, 2021.
http://hdl.handle.net/2142/100979.
MLA Handbook (7th Edition):
Ansari, Ali. “Engineering and optimizing physiological cell isolation via the secondary anchor targeted cell release system.” 2018. Web. 17 Apr 2021.
Vancouver:
Ansari A. Engineering and optimizing physiological cell isolation via the secondary anchor targeted cell release system. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2142/100979.
Council of Science Editors:
Ansari A. Engineering and optimizing physiological cell isolation via the secondary anchor targeted cell release system. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/100979
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Open Access Theses and Dissertations
