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University of Illinois – Chicago
1.
Seal, Alexandra A.
Viral MicroRNAs Modulate Transcriptome in Oral Keratinocytes and Alter Cytokine Levels in Myeloid Cells.
Degree: 2017, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/21736 
► MicroRNAs (miRNAs) are small, non-coding RNAs of ~18-25 nucleotides that have gained extensive attention as critical regulators in complex gene networks. Some of the key…
(more)
▼ MicroRNAs (miRNAs) are small, non-coding RNAs of ~18-25 nucleotides that have gained extensive attention as critical regulators in complex gene networks. Some of the key regulations involve immune cell lineage commitment, differentiation, maturation, and maintenance of immune homeostasis and function. Many viruses encode miRNAs that directly modulate expression of genes of the innate immune system, which includes proteins involved in promoting apoptosis and recruitment of cells of the immune system. In this study, we examined the expression profiles of three known v-miRNAs (vmiRs) from HSV-1 (miR-H1), KSHV (miR-K12-3), and HCMV (miR-US4) in healthy and diseased periodontal tissues from non-obese and obese subjects and observed increased levels of these vmiRs in diseased tissues compared to respective controls. Next, we investigated the impact of miR-H1 and miR-K12-3 overexpression on the host transcriptome focusing on gingival epithelial cells that are target sites for various HHVs. Expression of more than 1300 genes were altered in human oral keratinocytes (HOK) transfected with miR-H1 and miR-K12-3. Global pathway analysis identified dysregulation of key signaling genes and pathways that may favor virus persistence. Using bioinformatic analysis, we identified hundreds of potential vmiR binding sites on genes downregualted by miR-H1 and miR-K12-3 suggesting direct regualtion of these genes by vmiRs. We also assessed the expression of six selected vmiR-deregulated genes in myeloid inflammtory cells viz., macrophages and dendritic cells. Our results show that three genes exhibit similar changes in myeloid cells as observed in HOK while the remaining genes exhibit cell specific changes.
Finally, cytokine responses of vmiR expressing myeloid cells challenged with lipopolysacchride (LPS) derived from the periodontal pathogen, Aggregatibacter actinomycetemcomitans (Aa) show increased levels of TNF-α but reduced IL-12p40 indicating immunomoduatory functions of vmiRs. Overall, our resutls demonstrate clinical and functional relevance of pathogenic viral molecules viz., vmiRs capable of modulating expression of key gene and pathways in HOK and cytokine expression in myeloid cells which may favor an environment condusive to periodontal pathology.
Advisors/Committee Members: Nares, Salvador (advisor), Atsawasuwam, Phimon (committee member), Luan, Xianghong (committee member), Zhou, Xiaofeng (committee member), Naqvi, Afsar (committee member), Nares, Salvador (chair).
Subjects/Keywords: MiRNAs; MicroRNAs; VmiRNAs
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APA (6th Edition):
Seal, A. A. (2017). Viral MicroRNAs Modulate Transcriptome in Oral Keratinocytes and Alter Cytokine Levels in Myeloid Cells. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21736
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Seal, Alexandra A. “Viral MicroRNAs Modulate Transcriptome in Oral Keratinocytes and Alter Cytokine Levels in Myeloid Cells.” 2017. Thesis, University of Illinois – Chicago. Accessed April 13, 2021.
http://hdl.handle.net/10027/21736.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Seal, Alexandra A. “Viral MicroRNAs Modulate Transcriptome in Oral Keratinocytes and Alter Cytokine Levels in Myeloid Cells.” 2017. Web. 13 Apr 2021.
Vancouver:
Seal AA. Viral MicroRNAs Modulate Transcriptome in Oral Keratinocytes and Alter Cytokine Levels in Myeloid Cells. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/10027/21736.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Seal AA. Viral MicroRNAs Modulate Transcriptome in Oral Keratinocytes and Alter Cytokine Levels in Myeloid Cells. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/21736
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Chicago
2.
Shango, Jennifer G.
Modulation of Oral Immunity by Viral MicroRNA: Impact on Macrophage & Epithelial Cell MicroRNA Expression.
Degree: 2016, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/21336
► MicroRNAs (miRNAs) are small, 18-25 nucleotide non-coding RNAs. They They bind to 3’UTRs of target mRNA to regulate gene expression to causing translational silencing and…
(more)
▼ MicroRNAs (miRNAs) are small, 18-25 nucleotide non-coding RNAs. They They bind to 3’UTRs of target mRNA to regulate gene expression to causing translational silencing and destabilization of mRNA. miRNAs are required for normal immune development and function. When there is aberrant expression of miRNAs, there dysregulated innate and adaptive immune responses. Viral miRNAs (v-miRs) have been identified in a number of human viruses. This study focused on vmiRs from herpes simplex virus-1 (HSV-1), human cytomegalovirus (HCMV), and Kapsosi Sarcoma humanvirus (KSHV). This is an in-vitro study to investigate the relationship between vmiRs derived from HCMV, KSHV, and HSV-1 and host miRNA from human macrophages (Mφ) and human gingival epithelial cells (HGEC). We hypothesize these vmiRs will target host miRNAs directly related to inflammation and pathogenesis. We would like to identify host and vmiRs that are expressed or repressed by this interaction. We isolated and differentiated Mφ and HGECs. We preformed transfections with three viral mimics: K12-3-3p (KSHV), miR-H1 (HSV-1), and UL-70-3p (HCMV). The total RNA was isolated and miRNA profiling was preformed using Exiqon Services using 7th generation microarrays. We found that there is altered expression of several cellular miRNAs in both HGEC and Mφ transfected with miR-H1 and miR-K12-3-3p. However, for miR-UL70-3p, very few miRNAs were impacted significantly. We found that the overexpression of vmiRs alter the expression of cellular miRNAs. These cellular miRNAs were predicted to regulate numerous cellular pathways related, but not limited to cell movement, signaling pathways, endocytosis, cell survival and proliferation, etc. We found the expression of miR-K12-3-3p can interfere with the cell migration in HGECs. We also found that phagocytosis and cytokine secretion was monitored in Mφ transfected vmiRs miR-H1- and miR-K12-3-3p-mediated inhibition the both processes. Finally, we identified and confirmed two novel target of vmiRs showing miR-UL-70-3p and miR-K12-3-3p directly bind to the 3′UTR of MMP-24 and FOS, respectively. Our findings show that vmiRs can subvert the hosts’ innate imune responses.
Advisors/Committee Members: Nares, Salvador (advisor), Sroussi, Herve (committee member), Zhou, Xiaofeng (committee member).
Subjects/Keywords: miRNAs; viral miRNAs; oral immunity
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APA ·
Chicago ·
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CSE |
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to Zotero / EndNote / Reference
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APA (6th Edition):
Shango, J. G. (2016). Modulation of Oral Immunity by Viral MicroRNA: Impact on Macrophage & Epithelial Cell MicroRNA Expression. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21336
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shango, Jennifer G. “Modulation of Oral Immunity by Viral MicroRNA: Impact on Macrophage & Epithelial Cell MicroRNA Expression.” 2016. Thesis, University of Illinois – Chicago. Accessed April 13, 2021.
http://hdl.handle.net/10027/21336.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shango, Jennifer G. “Modulation of Oral Immunity by Viral MicroRNA: Impact on Macrophage & Epithelial Cell MicroRNA Expression.” 2016. Web. 13 Apr 2021.
Vancouver:
Shango JG. Modulation of Oral Immunity by Viral MicroRNA: Impact on Macrophage & Epithelial Cell MicroRNA Expression. [Internet] [Thesis]. University of Illinois – Chicago; 2016. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/10027/21336.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shango JG. Modulation of Oral Immunity by Viral MicroRNA: Impact on Macrophage & Epithelial Cell MicroRNA Expression. [Thesis]. University of Illinois – Chicago; 2016. Available from: http://hdl.handle.net/10027/21336
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Chicago
3.
Lu, Angela.
Orthodontic Tooth Movement in MicroRNA-29 Sponge Transgenic Mice.
Degree: 2017, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/21789
► Orthodontic tooth movement (OTM) occurs when a mechanical load is applied to a tooth through an appliance. Changes in these adaptive responses to orthodontic force…
(more)
▼ Orthodontic tooth movement (OTM) occurs when a mechanical load is applied to a tooth through an appliance. Changes in these adaptive responses to orthodontic force depend on activities of periodontal ligament cells, osteoblasts, osteocytes and osteoclasts, which are influenced by cellular expression of regulatory genes at the right location for a given time. This is a sophisticated process that involves bone resorption at the sites of pressure and bone deposition on the tension side with a tight regulation at post-transcriptional and post-translational stages. To gain an insight into mechanisms that regulate the tooth movement at a molecular level will lead to an effective approach for clinicians to improve orthodontic care.
MicroRNAs (miRNAs) are short non-coding RNAs that are emerging as important regulators of post-transcriptional gene expression in osteoblastogenesis and osteoclastogenesis. Emerging evidence has indicated that specific miRNAs play a crucial role in osteoclastogenesis and osteoclast differentiation. In this study, we focus on the miR-29 family, which expresses in different patterns corresponding to different orientations of forces in human periodontal ligament cells. In addition, miR-29 family plays important roles in osteoblasts/clasts differentiation and functions.
The aim of the study is to evaluate the tooth movement phenotype of miR-29 sponge mice (miR-29 underexpressor) and gain insights into the possible mechanisms of how miR-29 family plays a role in tooth movement via osteoclast function; and to study the histological change and cellular effects from miR-29 loss of function. The tooth movement was achieved using a 5-cN expansion spring on a mandibular first molar of miR-29 sponge mice. After 2 weeks, the distance of tooth movement was evaluated using Faxitron® radiograph and a standardized gauge in microcomputed tomogram, H&E and TRAP staining were performed to investigate the activity of osteoclasts during tooth movement. In addition, RT-realtime PCR was performed to evaluate the level of expression of miRNA in each animal. The average distance of tooth movement in the miR-29 sponge transgenic mice were significantly less than the one in the wildtype control mice. The expression of miR-29 family in the miR-29 sponge mice were 0.2-0.6 folds lower than the ones in the control mice. In addition, the numbers of osteoclasts in the miR-29 sponge mice were less than in the control mice. The findings of this study reveal a previously unknown realm that a miRNA can modulate OTM via osteoclast function
Advisors/Committee Members: Atsawasuwan, Phimon (advisor), Nares, Salvador (committee member), Zhou, Xiaofeng (Charles) (committee member), Viana, Grace (committee member), Atsawasuwan, Phimon (chair).
Subjects/Keywords: microRNA; orthodontic tooth movement; osteoclasts
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lu, A. (2017). Orthodontic Tooth Movement in MicroRNA-29 Sponge Transgenic Mice. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21789
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lu, Angela. “Orthodontic Tooth Movement in MicroRNA-29 Sponge Transgenic Mice.” 2017. Thesis, University of Illinois – Chicago. Accessed April 13, 2021.
http://hdl.handle.net/10027/21789.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lu, Angela. “Orthodontic Tooth Movement in MicroRNA-29 Sponge Transgenic Mice.” 2017. Web. 13 Apr 2021.
Vancouver:
Lu A. Orthodontic Tooth Movement in MicroRNA-29 Sponge Transgenic Mice. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/10027/21789.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lu A. Orthodontic Tooth Movement in MicroRNA-29 Sponge Transgenic Mice. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/21789
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Chicago
4.
Finkielsztein, Jessica.
Factors that Lead to Additional Surgical Interventions after Initial Exposure of Impacted Teeth.
Degree: 2019, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/23712
► Hypothesis: We hypothesize that the leading factor leading to revision of an exposure of an impacted tooth is debonding. Objective: Our objective is to identify…
(more)
▼ Hypothesis: We hypothesize that the leading factor leading to revision of an exposure of an impacted tooth is debonding. Objective: Our objective is to identify potential factors that lead to additional surgical interventions and problems after initial exposure of impacted teeth to reduce the number of revisions necessary. Methods: In order to find the records to do this retrospective study, we conducted a search of Axium records. We wanted to look at patients that had exposure of impacted teeth at the COD from January 1, 2003 to December 31, 2017. We sought the following combination of codes: duplicate of one of these codes with the same tooth site: D7281(old code used for exposures) ,D7283 (place device to aid eruption),D7280 (surgical exposure for orthodontic reasons), and then another search for one of those same codes but with the addition of either D4268 (surgical revision). In total we found 126 patient charts meeting the criteria. 14 of the charts were omitted due to either patient dismissal from the COD, coding errors or note inaccessibility. A total of 112 charts were included. Of the charts, 7 had two sites in which re-exposures were needed in two different sites. A total of 119 expose and bond revisions were evaluated. Results: The most common tooth that needed the revision was tooth6 (35.3%), second most was #11 (23.5%). The most common noted reasons for the revisions were as follows: 31.9% debonding, 22.7% gingival overgrowth, 16% ankylosis, and 5.9% required positional change of the attachment on the tooth. Conclusion: The most common need for a revision of an orthodontic tooth exposure was debonding, followed by gingival overgrowth. Funding: Not applicable IRB/ACC protocol: IRB 2018-5066
Advisors/Committee Members: Nares, Salvador (committee member), Goben, Abigail (committee member), Handelman, Chester (committee member), Viana, Grace (committee member), Schmerman , Michael (chair).
Subjects/Keywords: Orthodontics; Surgical Revisions; Impacted Teeth
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Finkielsztein, J. (2019). Factors that Lead to Additional Surgical Interventions after Initial Exposure of Impacted Teeth. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/23712
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Finkielsztein, Jessica. “Factors that Lead to Additional Surgical Interventions after Initial Exposure of Impacted Teeth.” 2019. Thesis, University of Illinois – Chicago. Accessed April 13, 2021.
http://hdl.handle.net/10027/23712.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Finkielsztein, Jessica. “Factors that Lead to Additional Surgical Interventions after Initial Exposure of Impacted Teeth.” 2019. Web. 13 Apr 2021.
Vancouver:
Finkielsztein J. Factors that Lead to Additional Surgical Interventions after Initial Exposure of Impacted Teeth. [Internet] [Thesis]. University of Illinois – Chicago; 2019. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/10027/23712.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Finkielsztein J. Factors that Lead to Additional Surgical Interventions after Initial Exposure of Impacted Teeth. [Thesis]. University of Illinois – Chicago; 2019. Available from: http://hdl.handle.net/10027/23712
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Chicago
5.
Ghoreishi, Reihaneh S.
Assessment of Literature Review Teaching Methods in Periodontics Programs in North America.
Degree: 2019, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/23745
► Hypothesis: We first hypothesize that there is diversity of teaching methods and resources available for residents in periodontics programs through North America. Secondly, we want…
(more)
▼ Hypothesis: We first hypothesize that there is diversity of teaching methods and resources available for residents in periodontics programs through North America. Secondly, we want to discover the top articles listed by different periodontics program in order to unify of content for teaching in North America.
Objective: To provide information regarding teaching methods and contents in order to prepare residents for ABP in every program.
Methods: Part one: The initial survey was sent to the 63 Periodontics Department Program Directors of American and Canadian schools. Included was an 8-page questionnaire and blank flash drive to be used in transmitting detailed literature review subjects and articles. The responding schools were then categorized into five different regions: 1- East, 2- West, 3- Midwest, 4- South, 5- Canada. Reponses were given a numeric code with the identification of the school linked to the code stored separately to ensure program confidentiality.
Part two: The original excel spread sheet was composed of 6 columns in order of article title, school number, region, institution’s choice of topics, and classification based on classic/ current/ or implant literature. In order to unify the referencing layout for all articles, the American Medical Association (AMA) format was used.
Results: The results show no significant differences between selected regions with regards to teaching methodology of classical, contemporary, implant literature review courses. Formulated cross tabulation reveals different frequencies in individual responses. However, it clearly reveals significant differences in the contents of the courses.
Conclusions: This research emphasizes the need to unify and comprehensively set forth a protocol in selecting the contents of literature reviewed for every resident at every level of their education. In addition, providing consolidated and comprehensive course contents can help every resident in national exam preparations.
Advisors/Committee Members: Schmerman, Michael (advisor), Ashrafi, Seema (advisor), Nares, Salvador (committee member), Goben, Abigail H (committee member), Viana, Maria Grace (committee member), Schmerman, Michael (chair).
Subjects/Keywords: Classic Literature Review; Periodontal Literature Review in North America; In-Service Exam; Periodontics Education; Bibliography in Periodontology; Teaching Method in periodontology
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Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ghoreishi, R. S. (2019). Assessment of Literature Review Teaching Methods in Periodontics Programs in North America. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/23745
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ghoreishi, Reihaneh S. “Assessment of Literature Review Teaching Methods in Periodontics Programs in North America.” 2019. Thesis, University of Illinois – Chicago. Accessed April 13, 2021.
http://hdl.handle.net/10027/23745.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ghoreishi, Reihaneh S. “Assessment of Literature Review Teaching Methods in Periodontics Programs in North America.” 2019. Web. 13 Apr 2021.
Vancouver:
Ghoreishi RS. Assessment of Literature Review Teaching Methods in Periodontics Programs in North America. [Internet] [Thesis]. University of Illinois – Chicago; 2019. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/10027/23745.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ghoreishi RS. Assessment of Literature Review Teaching Methods in Periodontics Programs in North America. [Thesis]. University of Illinois – Chicago; 2019. Available from: http://hdl.handle.net/10027/23745
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
. 
Open Access Theses and Dissertations