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You searched for +publisher:"University of Guelph" +contributor:("Nagy, Eva"). Showing records 1 – 3 of 3 total matches.

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University of Guelph

1. Grgic, Helena. Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection .

Degree: 2012, University of Guelph

The family Adenoviridae consists of five genera, including the genus Aviadenovirus, which infects avian species. The genus Aviadenovirus currently comprises five fowl (Fowl adenovirus A-E), one falcon (Falcon adenovirus A), and one goose (Goose adenovirus) adenovirus species. Fowl adenoviruses (FAdVs) have a worldwide distribution. Some are associated with diseases such as inclusion body hepatitis (IBH), while FAdV species C serotype 4 (FAdV-4) has been associated with hydropericardium-hepatitis syndrome (HHS). In this study, the complete nucleotide sequence of fowl adenovirus serotype 8 (FAdV-8) was determined. The full genome was 44,055 nucleotides (nt) in length, with an organization similar to that of the FAdV-1 and FAdV-9 genomes. No regions homologous to early regions E1, E3, and E4 of mastadenoviruses were recognized Pathogenicity of FAdV-8 and FAdV-4 were studied in specific-pathogen-free chickens following oral and intramuscular inoculations. Pathogenicity was determined on the basis of clinical signs and gross and histological lesions. Additionally, virus shedding and viral genome copy numbers in liver, cecal tonsil, and bursa of Fabricius were determined. The role of interleukins (IL) in the pathogenicity of and immune response to FAdVs is unknown. Therefore, in a chicken experiment, interferon-γ, IL-10, IL-18, and IL-8 gene expression was evaluated following FAdV-8 and FAdV-4 infection. Cytokine gene expression was examined in the liver, spleen, and cecal tonsils. This study explored the ability of fowl adenoviruses to subvert the host cell’s secretion of cytokines in response to infection as an important viral mechanism for immune evasion during infection. Variations in virulence of FAdVs are likely to be determined by the fiber alone as shown by Pallister et al. (1996). Therefore, we compared and analyzed the nt and amino acid (aa) sequences of the fiber gene of pathogenic and non-pathogenic FAdVs representing species groups D (FAdV-11) and E (FAdV-8). According to our data, virulence might not be associated only with sequence of the fiber gene. This work is a continuation of our efforts towards better understanding of the molecular biology of FAdVs and the pathogenesis of the disease, with an emphasis on the role of interleukins, an unknown area. Advisors/Committee Members: Nagy, Eva (advisor).

Subjects/Keywords: Sequence analysis; pathogenicity; cytokine gene expression patterns

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Grgic, H. (2012). Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection . (Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Grgic, Helena. “Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection .” 2012. Thesis, University of Guelph. Accessed November 22, 2019. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Grgic, Helena. “Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection .” 2012. Web. 22 Nov 2019.

Vancouver:

Grgic H. Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection . [Internet] [Thesis]. University of Guelph; 2012. [cited 2019 Nov 22]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Grgic H. Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection . [Thesis]. University of Guelph; 2012. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of Guelph

2. Ackford, James. Optimizing Foreign Gene Expression in Recombinant Fowl Adenovirus 9 Vectors .

Degree: 2015, University of Guelph

Our laboratory focuses on the molecular characterization of a non-pathogenic strain of fowl adenovirus (FAdV) 9 and its development as a versatile vaccine vector platform. The objectives of this study were to optimize transgene expression by recombinant viruses. High expression promoters and a post-transcriptional regulatory element were evaluated for their ability to improve expression of enhanced green fluorescent protein (EGFP) in recombinant FAdVs. These findings were compared to our current system that employs the human cytomegalovirus (CMV) promoter to express a transgene. EGFP expression was assessed by fluorometry and Western blots. A synthetic CMV enhancer/chicken β-actin (CAG) promoter and the human elongation factor 1 alpha (EF1α) promoter significantly increased expression of EGFP compared to the CMV promoter. However, expression was significantly decreased in the presence of woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). The results provide novel insight into avian vaccine design and optimization of transgene expression by FAdV vectors. Advisors/Committee Members: Nagy, Eva (advisor), Krell, Peter (advisor).

Subjects/Keywords: fowl adenovirus; vector; vaccine; poultry; expression; promoter

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ackford, J. (2015). Optimizing Foreign Gene Expression in Recombinant Fowl Adenovirus 9 Vectors . (Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9083

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ackford, James. “Optimizing Foreign Gene Expression in Recombinant Fowl Adenovirus 9 Vectors .” 2015. Thesis, University of Guelph. Accessed November 22, 2019. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9083.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ackford, James. “Optimizing Foreign Gene Expression in Recombinant Fowl Adenovirus 9 Vectors .” 2015. Web. 22 Nov 2019.

Vancouver:

Ackford J. Optimizing Foreign Gene Expression in Recombinant Fowl Adenovirus 9 Vectors . [Internet] [Thesis]. University of Guelph; 2015. [cited 2019 Nov 22]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9083.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ackford J. Optimizing Foreign Gene Expression in Recombinant Fowl Adenovirus 9 Vectors . [Thesis]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9083

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Diaz-Mendez, Andres. Characterization of an Equine Rhinitis A Virus (ERAV/ON/05) and Development of an Experimental Infection Model in Horses .

Degree: 2012, University of Guelph

In 2005 an equine rhinitis A virus (ERAV) isolate was recovered from a febrile horse during a respiratory outbreak in Ontario. This isolate (ERAV/ON/05) was propagated in cell culture and used to study its genomic characteristics and to investigate the clinical features in experimentally infected ponies. The fulllength genome of this isolate was sequenced and compared with other ERAV available in GenBank. The isolate genome is 7839 nucleotides (nts) in length with a variable 5’UTR and a more conserved 3’UTR. When the isolate was compared to other reported ERAV, an insertion of 13 nts in the 5’UTR was identified. Phylogenetic analysis demonstrated that ERAV/ON/05 was closely related to the ERAV/PERV isolate, which was recovered in 1962 in the United Kingdom. An experimental model was developed to study the clinical infection in naïve healthy ponies (ERAV/ON/05 n=4 and placebo n=4). ERAV/ON/05 induced clinical respiratory disease compared to placebo. The clinical signs consisted of pyrexia, nasal discharge, increased and abnormal lung sounds, increased size of submandibular lymph nodes and persistent mucopus in the trachea (up to 21 days post-infection). The virus was isolated from the lower and upper airways up to day 7 post-infection, corresponding with the detection of neutralizing ERAV antibodies. Assessment of the cytokine profile from bronchoalveolar lavage (BAL) cells demonstrated that this infection induced down-regulation of the mRNA expression of IL-4. One year later, four previously infected ponies with neutralizing antibodies to ERAV were assigned to a reinfection trial. None of the re-infected ponies developed clinical disease, and only one animal had a four-fold increase in antibody titres to ERAV. Attempts to recover the virus from the re-infected ponies using cell culture were negative; however, a down-regulation of the mRNA expression of IL-4 and IFN-β was identified in BAL cells. In conclusion, this study shows that the genome of ERAV has not significantly changed in the last 50 years and more importantly the virus induces clinical respiratory disease similar to other common equine respiratory viruses. Advisors/Committee Members: Viel, Laurent (advisor), Nagy, Eva (advisor).

Subjects/Keywords: Equine; Rhinitis virus; genome sequencing; infection model

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Diaz-Mendez, A. (2012). Characterization of an Equine Rhinitis A Virus (ERAV/ON/05) and Development of an Experimental Infection Model in Horses . (Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Diaz-Mendez, Andres. “Characterization of an Equine Rhinitis A Virus (ERAV/ON/05) and Development of an Experimental Infection Model in Horses .” 2012. Thesis, University of Guelph. Accessed November 22, 2019. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Diaz-Mendez, Andres. “Characterization of an Equine Rhinitis A Virus (ERAV/ON/05) and Development of an Experimental Infection Model in Horses .” 2012. Web. 22 Nov 2019.

Vancouver:

Diaz-Mendez A. Characterization of an Equine Rhinitis A Virus (ERAV/ON/05) and Development of an Experimental Infection Model in Horses . [Internet] [Thesis]. University of Guelph; 2012. [cited 2019 Nov 22]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Diaz-Mendez A. Characterization of an Equine Rhinitis A Virus (ERAV/ON/05) and Development of an Experimental Infection Model in Horses . [Thesis]. University of Guelph; 2012. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.