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You searched for +publisher:"University of Guelph" +contributor:("Frances, Sharom"). One record found.

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University of Guelph

1. Clay, Adam Thomas. The Role of the Lipid Bilayer in P-glycoprotein Drug Binding, Transport and Catalytic Functions .

Degree: 2011, University of Guelph

The ABC protein P-glycoprotein (Pgp, ABCB1) transports many structurally diverse substrates from the lipid bilayer. Previous studies demonstrated the importance of the membrane environment, but few have quantified these effects. In the present work, purified Pgp reconstituted into defined lipid systems was employed. Drug binding affinities were determined using Trp quenching, and drug-lipid partitioning by equilibrium dialysis. Pgp bound substrates from the bilayer with affinities in the millimolar range; both drug-Pgp and drug-lipid interactions were important. The kinetics of Pgp-mediated drug transport were sensitive to drug structure and lipid environment. The rate of transport is proposed to depend on the affinity of Pgp for substrate and conformational changes. The lipid bilayer affected the stability of Pgp catalytic activity which provided evidence for distinct basal and drug-stimulated ATPase cycles. Overall, the lipid environment had pronounced effects on Pgp-mediated drug binding, transport and catalytic functions. Advisors/Committee Members: Frances, Sharom (advisor).

Subjects/Keywords: P-glycoprotein; Pgp; ABCB1; drug binding; drug transport

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Clay, A. T. (2011). The Role of the Lipid Bilayer in P-glycoprotein Drug Binding, Transport and Catalytic Functions . (Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3196

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Clay, Adam Thomas. “The Role of the Lipid Bilayer in P-glycoprotein Drug Binding, Transport and Catalytic Functions .” 2011. Thesis, University of Guelph. Accessed June 18, 2019. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3196.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Clay, Adam Thomas. “The Role of the Lipid Bilayer in P-glycoprotein Drug Binding, Transport and Catalytic Functions .” 2011. Web. 18 Jun 2019.

Vancouver:

Clay AT. The Role of the Lipid Bilayer in P-glycoprotein Drug Binding, Transport and Catalytic Functions . [Internet] [Thesis]. University of Guelph; 2011. [cited 2019 Jun 18]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3196.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Clay AT. The Role of the Lipid Bilayer in P-glycoprotein Drug Binding, Transport and Catalytic Functions . [Thesis]. University of Guelph; 2011. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3196

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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