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You searched for +publisher:"University of Connecticut" +contributor:("Dr. Qiang Zhu, Dr. Kamran Safavi"). Showing records 1 – 2 of 2 total matches.

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University of Connecticut

1. Blondin, Joseph M. Purification and Biological Activity of Serine Lipids of Porphyromonas endodontalis.

Degree: MS, Dental Science, 2014, University of Connecticut

Porphymonas endodontalis has been shown to populate necrotic root canal systems and may contribute to apical bone loss. Recent work has shown that P. endodontalis synthesizes a novel serine lipid class (Lipid 654) similar to that produced by P. gingivalis. Objective: The purpose of this study was to isolate P. endodontalis Lipid 654 in very high purity and characterize its structure and biological activity
 in RAW 264.7 cells and HEK293 cells. Methods: P. endodontalis (ATCC 35406, type strain) was grown under anaerobic conditions and centrifuged. P. endodontalis total lipids were recovered using the Bligh and Dyer phospholipid extraction procedure and were fractionated by semipreparative normal phase HPLC using hexane-isopropanol-water (6:8:0.75, vol/vol/vol). Fractions containing Lipid 654 were identified by mass spectrometric (MS) analysis and were pooled, refractionated using an acidic HPLC solvent, and tested for biological activity. Results: MS analysis revealed that repurified Lipid 654 contained P. endodontalis and P. gingivalis Lipid 654 preparations and was significantly attenuated by anti-mouse TLR2 neutralizing antibody. Treatment of HEK293 cells, stably transfected to express TLR2 and an NFκB-dependent secretory alkaline phosphatase reporter, revealed that both P. endodontalis and P. gingivalis Lipid 654 preparations engage human TLR2 with equal potency. Conclusions: Our results show that P. endodontalis Lipid 654 engages TLR2 with stimulation of TNF-α secretion from macrophages and activation of the transcription factor NF-κB, and these responses may contribute to apical bone destruction associated with necrotic pulps. Advisors/Committee Members: Dr. Qiang Zhu, Dr. Kamran Safavi, Dr. Frank Nichols.

Subjects/Keywords: Purification; Serine; Lipid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Blondin, J. M. (2014). Purification and Biological Activity of Serine Lipids of Porphyromonas endodontalis. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/619

Chicago Manual of Style (16th Edition):

Blondin, Joseph M. “Purification and Biological Activity of Serine Lipids of Porphyromonas endodontalis.” 2014. Masters Thesis, University of Connecticut. Accessed October 17, 2019. https://opencommons.uconn.edu/gs_theses/619.

MLA Handbook (7th Edition):

Blondin, Joseph M. “Purification and Biological Activity of Serine Lipids of Porphyromonas endodontalis.” 2014. Web. 17 Oct 2019.

Vancouver:

Blondin JM. Purification and Biological Activity of Serine Lipids of Porphyromonas endodontalis. [Internet] [Masters thesis]. University of Connecticut; 2014. [cited 2019 Oct 17]. Available from: https://opencommons.uconn.edu/gs_theses/619.

Council of Science Editors:

Blondin JM. Purification and Biological Activity of Serine Lipids of Porphyromonas endodontalis. [Masters Thesis]. University of Connecticut; 2014. Available from: https://opencommons.uconn.edu/gs_theses/619


University of Connecticut

2. Mainkar, Anshul. Proportion of MB2 Canals Identified at Different Depths of Excavation in Extracted Teeth and Comparison with CBCT Prediction.

Degree: Master of Dental Science, Dental Science, 2019, University of Connecticut

Objective: The purpose of this study is to evaluate the depth of troughing needed to locate the mesiobuccal 2 canals in maxillary molars. Troughing the pulp chamber floor can locate hidden or calcified mesio-lingual canals. Materials and Methods: This study utilized 29 extracted maxillary first and second molars with mesiobuccal 2 canals confirmed by sectioning the mesiobuccal root in cross-section 5 mm coronal to the apex. A standard access cavity was prepared and mesiobuccal, distobuccal, and palatal canals were located. A groove was troughed palatal to the mesiobuccal orifice and slightly mesial to the line connecting mesiobuccal and palatal canal orifices until the mesiobuccal 2 canal was visually identified and confirmed with an endodontic file. Troughing depth was determined using digital calipers. Results: The depth of troughing needed to locate the mesio-lingual canal ranged from 0.04 to 2.12 mm. Five teeth needed to be troughed less than 0.5 mm and two teeth needed to be troughed more than 2.0 mm to identify the mesio-lingual canal. Conclusion: In majority of cases, 0.5 mm troughing was sufficient to locate mesio-lingual canals. Clinicians will miss some mesio-lingual canals if troughing is limited to less than 2.0 mm. Advisors/Committee Members: Dr. Qiang Zhu, Dr. Kamran Safavi, Dr. Yu-Hsiung Wang, Qiang Zhu.

Subjects/Keywords: MB2; endodontics; root canal; maxillary molar

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mainkar, A. (2019). Proportion of MB2 Canals Identified at Different Depths of Excavation in Extracted Teeth and Comparison with CBCT Prediction. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/1402

Chicago Manual of Style (16th Edition):

Mainkar, Anshul. “Proportion of MB2 Canals Identified at Different Depths of Excavation in Extracted Teeth and Comparison with CBCT Prediction.” 2019. Masters Thesis, University of Connecticut. Accessed October 17, 2019. https://opencommons.uconn.edu/gs_theses/1402.

MLA Handbook (7th Edition):

Mainkar, Anshul. “Proportion of MB2 Canals Identified at Different Depths of Excavation in Extracted Teeth and Comparison with CBCT Prediction.” 2019. Web. 17 Oct 2019.

Vancouver:

Mainkar A. Proportion of MB2 Canals Identified at Different Depths of Excavation in Extracted Teeth and Comparison with CBCT Prediction. [Internet] [Masters thesis]. University of Connecticut; 2019. [cited 2019 Oct 17]. Available from: https://opencommons.uconn.edu/gs_theses/1402.

Council of Science Editors:

Mainkar A. Proportion of MB2 Canals Identified at Different Depths of Excavation in Extracted Teeth and Comparison with CBCT Prediction. [Masters Thesis]. University of Connecticut; 2019. Available from: https://opencommons.uconn.edu/gs_theses/1402

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