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You searched for +publisher:"University of Arizona" +contributor:("Turzillo, Adele M."). Showing records 1 – 2 of 2 total matches.

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University of Arizona

1. Valdez, Kelli E. The role of oxidative stress and nuclear factor-kappa B in the control of apoptosis and atresia in dominant bovine follicles .

Degree: 2004, University of Arizona

Apoptosis of granulosa cells is an early feature of atresia in bovine follicles. However, the mechanisms initiating apoptosis of granulosa cells have not been well characterized. In many cell types, apoptosis can be induced by oxidative stress and prevented by increased expression of endogenous oxidative stress response proteins. The transcription factor nuclear factor-kappaB (NF-κB) regulates genes involved in preventing oxidative stress and has been characterized as a mediator of cell survival. Studies have suggested that NF-κB activity may be regulated by estradiol-17β, a major determinant of follicular health. Therefore, these studies tested the hypotheses that oxidative stress is involved in the atresia of non-ovulatory dominant bovine follicles and NF-κB suppresses apoptosis in the healthy dominant follicle. Because the onset of atresia during the first wave of follicular development following ovulation has previously been characterized in cattle, it provides an excellent model to study the relationship between apoptosis, oxidative stress response proteins, NF-kappaB, and estradiol production. Decreased production of estradiol was accompanied by increased numbers of non-viable granulosa cells in dominant follicles between Days 4 and 6 of the first follicular wave in cattle. Unexpectedly, expression of genes encoding oxidative stress response proteins in granulosa cells increased on Day 8 of the follicular wave, but did not translate into increased expression of the corresponding proteins or greater enzyme activity. The decline in estradiol production observed on Day 6 of the 1st follicular wave is not due to lack of androgen substrate or down-regulated expression of the aromatase gene, but instead appears to be the direct result of decreased activity of the aromatase enzyme within granulosa cells. Follicular NF-κB activation was associated with estradiol production. However, direct inhibition of NF-κB activity suppressed apoptosis of granulosa cells in vitro. Therefore, whether NF-κB is playing a pro- or anti-apoptotic role in granulosa cells of bovine follicles remains to be elucidated. These experiments begin to elucidate the signals that trigger the sequence of events leading to apoptosis and atresia of bovine follicles and provide the framework for future experiments designed to further clarify the complex interplay of signals that determine a follicle's fate. Advisors/Committee Members: Turzillo, Adele M (advisor).

Subjects/Keywords: Biology; Animal Physiology.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Valdez, K. E. (2004). The role of oxidative stress and nuclear factor-kappa B in the control of apoptosis and atresia in dominant bovine follicles . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/290132

Chicago Manual of Style (16th Edition):

Valdez, Kelli E. “The role of oxidative stress and nuclear factor-kappa B in the control of apoptosis and atresia in dominant bovine follicles .” 2004. Doctoral Dissertation, University of Arizona. Accessed March 05, 2021. http://hdl.handle.net/10150/290132.

MLA Handbook (7th Edition):

Valdez, Kelli E. “The role of oxidative stress and nuclear factor-kappa B in the control of apoptosis and atresia in dominant bovine follicles .” 2004. Web. 05 Mar 2021.

Vancouver:

Valdez KE. The role of oxidative stress and nuclear factor-kappa B in the control of apoptosis and atresia in dominant bovine follicles . [Internet] [Doctoral dissertation]. University of Arizona; 2004. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10150/290132.

Council of Science Editors:

Valdez KE. The role of oxidative stress and nuclear factor-kappa B in the control of apoptosis and atresia in dominant bovine follicles . [Doctoral Dissertation]. University of Arizona; 2004. Available from: http://hdl.handle.net/10150/290132


University of Arizona

2. Moore, Heather Corrina. Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology .

Degree: 2006, University of Arizona

Goals of this dissertation were to 1) use custom-made cDNA microarrays to identify genes in the bovine pituitary gland that are differentially expressed during the estrous cycle and 2) characterize their patterns of gene expression. The estrous cycle is a dynamic process that requires coordination between the hypothalamus, pituitary gland, and ovaries. The anterior pituitary gland synthesizes and secretes the gonadotropins, luteinizing hormone (LH) and follicle stimulating hormone (FSH), which regulate steroidogenesis and follicular development. Currently, intrapituitary factors that modulate gonadotropin synthesis, storage, and release are not well described, thus, requiring investigation. To investigate the validity of the microarray results, we performed real-time PCR on 35 genes identified by cDNA microarray as being differentially regulated. Overall, microarray and real-time PCR results were consistent among our experiments suggesting that cDNA microarray is an efficacious tool for profiling gene expression in the bovine pituitary gland. Our first experiment was designed to identify genes that were regulated during the early luteal phase. This period is characterized by steadily increasing concentrations of progesterone (P4) from nadir to maximum. Samples from three different time points, d 2, d 6, and d 10 following initiation of the first follicular wave, were compared. One hundred and sixty nine genes were determined to be differentially expressed. Ten of these genes were validated using real-time PCR. The other two studies were designed to identify genes that were regulated during the preovulatory period as induced by the administration of prostaglandin F2α (PGF2α). This period is characterized by a decrease in circulating concentrations of P4 coincident with an increase in circulating concentrations of estradiol. Prior to the surge, FSH and LH are disconcordinately released but the underlying mechanisms regulating their release is unknown. The second study identified 1406 genes to be differentially regulated during the 72 h following administration of PGF2α. Twenty-seven of these transcripts were validated by real-time PCR. The third study identified 503 genes to be differentially regulated during the 48 h following administration of PGF2α. Twenty two of these transcripts were validated by real-time PCR. Together these experiments have identified several genes as potential intrapituitary factors that may function to regulate the reproductive axis. Advisors/Committee Members: Turzillo, Adele M. (committeemember), Hoyer, Patricia B. (committeemember), Hoying, James B. (committeemember), Braun, Eldon J. (committeemember), Baumgard, Lance H. (committeemember).

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Moore, H. C. (2006). Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/194108

Chicago Manual of Style (16th Edition):

Moore, Heather Corrina. “Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology .” 2006. Doctoral Dissertation, University of Arizona. Accessed March 05, 2021. http://hdl.handle.net/10150/194108.

MLA Handbook (7th Edition):

Moore, Heather Corrina. “Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology .” 2006. Web. 05 Mar 2021.

Vancouver:

Moore HC. Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology . [Internet] [Doctoral dissertation]. University of Arizona; 2006. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10150/194108.

Council of Science Editors:

Moore HC. Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology . [Doctoral Dissertation]. University of Arizona; 2006. Available from: http://hdl.handle.net/10150/194108

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