+publisher:"University of Adelaide" +contributor:("Langridge, Peter")

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University of Adelaide

1. Pinto González, Reyna Suzuky. Physiological traits and genetic controls associated with heat adaptation of bread wheat.

Degree: 2016, University of Adelaide

URL: http://hdl.handle.net/2440/119292

► High temperatures affect plant growth and reduce crop productivity. Notwithstanding the extensive research on the effects of heat stress on plant development, to date, the… (more)

▼ High temperatures affect plant growth and reduce crop productivity. Notwithstanding the extensive research on the effects of heat stress on plant development, to date, the basis of wheat tolerance to high temperatures is unknown. Currently, physiological crop breeding for improved wheat adaptation to heat stress is driven by the utilization of cumulative traits useful under those environments. However many of these traits have yet to be fully exploited. This study is focused on three key components of heat adaptation: I) the significance of root development for hot irrigated environments in comparison to drought stressed environments and its relation with canopy temperature, II) the contribution and genetic basis of the staygreen attribute to heat adaptation of bread wheat, and III) The relevance of leaf respiration for plant productivity under hot environments. Chapter 1 presents a literature review focused on providing a general background for the three following publications included in the forthcoming chapters. The relevance of heat stress for the wheat crop is discussed with focus on recent publications that describe the current situation and forecasts for the upcoming years and effect of climate change. The genetic and physiological basis of heat tolerance is also addressed, presenting a generic conceptual model currently applied in wheat breeding for improved heat adaptation. In Chapter 2, the significance of optimal root development under hot-irrigated environments is addressed and compared with the performance observed in the same germplasm grown under water-limited conditions. This journal publication discusses the association of QTL for canopy temperature with deep and abundant radicular systems. A subset of a Seri/Babax mapping population was evaluated for root distribution patterns and residual soil moisture across the soil profile from surface to 120 cm depth. Results from this study endorse canopy temperature as a valuable tool for assessing expression of plant’s water uptake and hence root development under both, hot-irrigated and drought environments. In Chapter 3, the potential value of the staygreen attribute in breeding for heat adaptation of wheat is addressed. This journal publication estimated a number of parameters associated with the staygreen phenotype including (but not limited to) the rate of senescence, the percentage of greenness decay and the area under the curve of the normalized difference vegetation index measured over time. The greenness decay of each genotype was fitted to linear or non- linear models to describe their patterns. And finally these staygreen parameters together with other physiological and agronomical parameters were analysed for QTL to aid understanding of the genetic basis of the staygreen attribute in the Seri/Babax population. Chapter 4 presents a descriptive study on leaf respiration rates of a diverse wheat germplasm set grown under high temperatures during the 2010, 2011, 2012 and 2013 seasons. Agronomic and physiological phenotyping was performed including… Advisors/Committee Members: Langridge, Peter (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: QTL; water-limited environment; heat tolerance; respiration; diversity; physiology; greenness; high temperature; spring wheat

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APA (6^th Edition):

Pinto González, R. S. (2016). Physiological traits and genetic controls associated with heat adaptation of bread wheat. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/119292

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pinto González, Reyna Suzuky. “Physiological traits and genetic controls associated with heat adaptation of bread wheat.” 2016. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/119292.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pinto González, Reyna Suzuky. “Physiological traits and genetic controls associated with heat adaptation of bread wheat.” 2016. Web. 15 Apr 2021.

Vancouver:

Pinto González RS. Physiological traits and genetic controls associated with heat adaptation of bread wheat. [Internet] [Thesis]. University of Adelaide; 2016. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/119292.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pinto González RS. Physiological traits and genetic controls associated with heat adaptation of bread wheat. [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/119292

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

2. Bi, Huihui. Characterization of wheat cuticle and wheat cuticle-related transcription factor genes in relation to drought.

Degree: 2016, University of Adelaide

URL: http://hdl.handle.net/2440/119641

► The plant cuticle forms a hydrophobic layer covering the epidermis of aerial organs of a plant, and plays important roles in plant development and protection… (more)

▼ The plant cuticle forms a hydrophobic layer covering the epidermis of aerial organs of a plant, and plays important roles in plant development and protection against environmental stresses. The primary role of the cuticle is to resist non-stomatal water loss. Several studies have explored the role of cuticle structure and composition in determining the cuticle’s ability to resist water loss. A number of transcription factor (TF) genes have been identified that regulate the biosynthesis of the cuticle, and many of these genes were found to play important roles in drought tolerance. However, little information was available on the drought-related composition, structure and function of the cuticle or on the regulatory genes involved in cuticle synthesis in wheat. This PhD project was designed to explore such relationships in wheat, to examine the impact of drought on the wheat leaf cuticle, and to identify and characterise drought-responsive wheat TF genes encoding regulators of cuticle biosynthesis. To fulfil the first two aims, residual transpiration rates, cuticle structure and cuticular wax composition were examined in five elite Australian wheat lines with contrasting glaucousness and drought tolerance, grown under conditions of sufficient or limited watering. Residual transpiration rates of non-glaucous and drought-sensitive Kukri were found to be much higher than those of the four glaucous and drought-tolerant lines, Excalibur, Drysdale, RAC875 and Gladius. No significant differences existed in the thickness of the cuticle between the five genotypes. Considerable variation was detected in the content of C31 β-diketone, which was well correlated with the respective levels of glaucousness. The amount of alkanes was increased under drought stress in all examined wheat lines while the thickness of the cuticle was increased in Drysdale and RAC875. Six wheat genes encoding MYB TFs and five homeologues of two wheat WXPL genes were cloned. Tissue specific and drought-responsive expression of four MYB genes and two WXPL homeologues were examined in Kukri and RAC875. The involvement of MYB and WXPL TFs in the regulation of cuticle biosynthesis was confirmed by their activation of the promoters derived from wheat genes encoding cuticle synthesis enzymes and the SHN1/WIN1 TF. Two functional MYB-responsive DNA cis-elements were localized in the TdSHN1 promoter, which was specifically activated by TaMYB74 but not by other MYB TFs characterised in this study. The different binding preferences of TaWXPL1D and TaWXPL2B for three stress-responsive DNA cis-elements were demonstrated using the yeast one-hybrid assay. Schemes which indicated the roles of wheat MYB and WXPL TFs in the regulation of cuticle biosynthetic genes under drought stress were proposed. To examine the function of TaSHN1 TF in wheat, transgenic wheat lines overexpressing the TaSHN1 gene were produced. Significant decreases were detected in the stomatal density on the abaxial surfaces of flag leaves of transgenic plants compared to control plants, grown under… Advisors/Committee Members: Langridge, Peter (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: Cuticle; cuticular wax; β-diketone; drought; glaucousness; residual transpiration rate; stomatal density; transcription factor; water deficit; wheat (Triticum aestivum)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bi, H. (2016). Characterization of wheat cuticle and wheat cuticle-related transcription factor genes in relation to drought. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/119641

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bi, Huihui. “Characterization of wheat cuticle and wheat cuticle-related transcription factor genes in relation to drought.” 2016. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/119641.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bi, Huihui. “Characterization of wheat cuticle and wheat cuticle-related transcription factor genes in relation to drought.” 2016. Web. 15 Apr 2021.

Vancouver:

Bi H. Characterization of wheat cuticle and wheat cuticle-related transcription factor genes in relation to drought. [Internet] [Thesis]. University of Adelaide; 2016. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/119641.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bi H. Characterization of wheat cuticle and wheat cuticle-related transcription factor genes in relation to drought. [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/119641

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

3. Ferdous, Jannatul. Identification of drought responsive microRNAs and functional analysis of a sample microRNA for drought tolerance in barley (Hordeum vulgare L.).

Degree: 2016, University of Adelaide

URL: http://hdl.handle.net/2440/113355

► Declining water resources coupled with the dwindling size of agricultural land necessitate the development of drought tolerant crop varieties. However, genetic control of drought tolerance… (more)

▼ Declining water resources coupled with the dwindling size of agricultural land necessitate the development of drought tolerant crop varieties. However, genetic control of drought tolerance is complex because of the involvement of a large number of genes. Studies in plant biotechnology for improved plant stress tolerance mostly concentrate on implementing and manipulating downstream gene, involved in the physiological responses. Recently, the upstream gene regulatory network involving small, non-coding RNAs such as microRNAs (miRNAs) and their target genes has been discovered. However, the drought regulatory relationship between miRNAs and their targets in barley, one of the major cereal crops, is still largely unknown. In this PhD project, potential drought responsive miRNAs and their targets were identified and validated in barley genotypes, and the drought tolerance of transgenic barley over-expressing miR827, a previously reported miRNA that conferred drought tolerance in Arabidopsis, was examined. To identify and validate drought responsive miRNAs and their targets, we conducted expression analysis of several drought responsive miRNAs under drought treatment in four barley genotypes that were reported to perform well in the drought prone areas of Australia. Differences in expression of four miRNAs; Ath-miR169b, Osa-miR1432, Hv-miRx5 and Hv-miR166b/c were observed between drought-treated and well-watered barley samples, and this expression varied among the experimental genotypes. Generally, miRNA-mediated cleavage of the target mRNAs was observed at the ideal cleavage site, however we also found miRNA-mediated cleavage not limited to the canonical position. We detected the spatial accumulation of potential drought responsive miRNAs and targets that mostly localized in the mesophyll tissues, and inverse correlation of expression between mature miRNAs and their target mRNAs. These results suggested a contribution of miRNAs to the molecular control of target gene expression, genotype-specific regulation under drought treatment, and the promise of miRNA: mRNA as biomarkers for genotypic selection in barley. To examine the drought tolerance of transgenic barley expressing miR827, we monitored the performance under drought of miR827 over-expressing transgenic barley plants under the control of the constitutive promoter CaMV-35S and drought-inducible promoter Zm-Rab17. We observed that the ectopic overexpression of Ath-miR827 resulted in unwanted side-effects, that is reduced shoot area, delayed anthesis and reduced whole plant water use efficiency (WUEwp) [wp subscript]and weight of seeds per plant in the transgenic compared with the wild type counterpart or null plants. In contrast, the drought inducible expression of Hv-miR827 caused several promising phenotypes; that is, the transgenic barley plants did not show reduced shoot area or delayed anthesis compared to the wild type, while the WUEwp [wp subscript] and leaf relative water content (RWC) were improved. Further, we observed the advantages of drought inducible… Advisors/Committee Members: Langridge, Peter (advisor), Tricker, Penny (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: barley; microRNAs; differential expression; drought stress; miR827; phenotyping; Research by Publication

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferdous, J. (2016). Identification of drought responsive microRNAs and functional analysis of a sample microRNA for drought tolerance in barley (Hordeum vulgare L.). (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/113355

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ferdous, Jannatul. “Identification of drought responsive microRNAs and functional analysis of a sample microRNA for drought tolerance in barley (Hordeum vulgare L.).” 2016. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/113355.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ferdous, Jannatul. “Identification of drought responsive microRNAs and functional analysis of a sample microRNA for drought tolerance in barley (Hordeum vulgare L.).” 2016. Web. 15 Apr 2021.

Vancouver:

Ferdous J. Identification of drought responsive microRNAs and functional analysis of a sample microRNA for drought tolerance in barley (Hordeum vulgare L.). [Internet] [Thesis]. University of Adelaide; 2016. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/113355.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ferdous J. Identification of drought responsive microRNAs and functional analysis of a sample microRNA for drought tolerance in barley (Hordeum vulgare L.). [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/113355

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

4. Tura, Habtamu. Towards the positional cloning of yield QTL on chromosome 1B for drought tolerance in wheat.

Degree: 2018, University of Adelaide

URL: http://hdl.handle.net/2440/127107

► Wheat feeds about 35% of the world’s population and its productivity needs to be increased by 2.8% every year. Drought and heat have been reported… (more)

▼ Wheat feeds about 35% of the world’s population and its productivity needs to be increased by 2.8% every year. Drought and heat have been reported to decrease grain yield up to 70 %. Fine mapping and dissecting the physiological effects of quantitative trait loci (QTL) for yield enable to find molecular markers and genes for breeding drought tolerant varieties. Here we focus on QTL with strong effects on yield and yield-related traits that were previously found on the chromosome 1B in three bread wheat (Triticum aestivum L.) mapping populations grown under abiotic stress. These QTL were first identified under low rain fed environments in three continents (Australia, Mexico and India) using double-haploid (DH) lines from Excalibur x Kukri (Edwards, 2012) and RAC875 x Kukri DH (Bennett et. al., 2012). The yield QTL on chromosome 1B of Excalibur x Kukri DH was highly expressed under severe drought stressed environments (yield < 500kg/ha) of South Australia and was not under the influence of phenology genes, and so was chosen for fine mapping. QTL for plant growth, relative leaf expansion and transpiration rate were also detected on chromosome 1B in a third mapping population derived from Drysdale x Gladius recombinant inbred lines (RIL), grown in glasshouse under well-watered and drought conditions, using a LemnaTec high throughput image phenotyping platform (Parent et al. (2015). The aims of this project were to: find whether or plant growth and transpiration QTL in Drysdale x Gladius coincide with a grain yield QTL, (2) fine map the yield QTL in Excalibur/Kukri near-isogenic lines (3) align the QTL from the three mapping populations onto chromosome 1B physical map and identify the candidate genes underlying yield under drought and heat stressed environments. For aim 1, we selected Drysdale/Gladius RIL with recombination points in the region covering the QTL for growth and transpiration rate previously reported by Parent et al. (2015). We tested these RIL for two consecutive years (2014 and 2015) under severe drought and heat stresses in a rainout shelter (polytunnel). We found a yield QTL on chromosome 1B which was co-located with QTL for seeds/spikelet, seeds/spike, biomass, spike weight and plant height. All were expressed specifically under severe drought and high temperatures. Drysdale was the positive allele for all the QTL, except for plant height, and had a strong effect on number of seeds/spike under severe drought and heat stressed in Drysdale/Gladius. We also found that the yield QTL match a QTL for relative leaf area expansion rate and partially overlap with a QTL for transpiration rate. We hypothesized that Drysdale allele at the 1B loci contributes to biomass accumulation at early growth stage leading to a yield increase under dry and hot climate. We aligned all the QTL for yield, yield components and physiological traits (growth, relative leaf expansion and transpiration rate) that were detected on the chromosome 1B in the three mapping populations onto the reference sequence RefSeq v1.0 and found… Advisors/Committee Members: Fleury, Delphine (advisor), Langridge, Peter (advisor), Garcia, Melissa (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: Triticum aestivum; molecular markers; heat tolerance; near isogenic lines; recombinant lines; candidate gene; genomic sequence

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tura, H. (2018). Towards the positional cloning of yield QTL on chromosome 1B for drought tolerance in wheat. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/127107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tura, Habtamu. “Towards the positional cloning of yield QTL on chromosome 1B for drought tolerance in wheat.” 2018. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/127107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tura, Habtamu. “Towards the positional cloning of yield QTL on chromosome 1B for drought tolerance in wheat.” 2018. Web. 15 Apr 2021.

Vancouver:

Tura H. Towards the positional cloning of yield QTL on chromosome 1B for drought tolerance in wheat. [Internet] [Thesis]. University of Adelaide; 2018. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/127107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tura H. Towards the positional cloning of yield QTL on chromosome 1B for drought tolerance in wheat. [Thesis]. University of Adelaide; 2018. Available from: http://hdl.handle.net/2440/127107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

5. Maphosa, Lancelot. Genetic control of grain quality in bread wheat (Triticum aestivum L.) grown under a range of environmental conditions.

Degree: 2013, University of Adelaide

URL: http://hdl.handle.net/2440/82085

► Abiotic stresses including high temperatures and moisture deficit are detrimental to bread wheat production. Under abiotic stresses, characteristics such as yield, growth rate, gene expression… (more)

▼ Abiotic stresses including high temperatures and moisture deficit are detrimental to bread wheat production. Under abiotic stresses, characteristics such as yield, growth rate, gene expression and quality are affected and responses might involve interaction of many genes. Most studies on the impact of abiotic stresses such as high temperatures and moisture deficit have concentrated on effects on yield and agronomic traits with less work being done on grain quality. This project focussed on the end-use quality of wheat grain produced under a range of field production conditions including high temperatures and water shortages, using two mapping populations, Gladius/Drysdale and RAC875/Kukri. Gladius, Drysdale and two pairs of backcross derivatives having Wyalkatchem and RAC1262A as recurrent parents were also studied under normal and heat stress conditions in a glasshouse experiment. Of the backcross derivatives, one line of each pair has a Gpc-B1 (high grain protein content) gene introgression and the other does not. Field trials were conducted in Australia and Mexico and the glasshouse experiment was conducted in Australia. For the glasshouse experiment, Gladius showed more heat tolerance with no significant decrease in grain weight compared to Drysdale. The backcross derivatives with the introgression segment had higher grain protein content, percentage unextractable polymeric protein and accelerated senescence than ones without the segment. Grain weight and senescence were severely affected by heat stress. Quality analysis of field grown material involved sequential assessment of grain, flour, dough and baked product characteristics. Stress conditions increased protein content, decreased yield, grain thickness, width and increased dough development time compared to the control. The exposure to heat stress resulted in an increase in loaf volume compared to the control experiment. Genetic linkage maps were constructed for the Gladius/Drysdale population and used for quantitative trait loci (QTL) analysis. Quantitative trait loci analysis detected several genomic regions associated with quality traits under a range of conditions including drought and heat stress in both populations. Some of the traits were associated with known phenology and quality genes, some QTLs detected have been reported in other studies but some QTLs were novel and had not been detected elsewhere. The novel QTLs detected under conditions involving heat and drought stress present opportunities for selection of lines that are able to maintain quality under these adverse conditions. Advisors/Committee Members: Mather, Diane Elizabeth (advisor), Langridge, Peter (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: genetics; wheat quality; drought stress; heat stress

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maphosa, L. (2013). Genetic control of grain quality in bread wheat (Triticum aestivum L.) grown under a range of environmental conditions. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/82085

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maphosa, Lancelot. “Genetic control of grain quality in bread wheat (Triticum aestivum L.) grown under a range of environmental conditions.” 2013. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/82085.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maphosa, Lancelot. “Genetic control of grain quality in bread wheat (Triticum aestivum L.) grown under a range of environmental conditions.” 2013. Web. 15 Apr 2021.

Vancouver:

Maphosa L. Genetic control of grain quality in bread wheat (Triticum aestivum L.) grown under a range of environmental conditions. [Internet] [Thesis]. University of Adelaide; 2013. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/82085.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maphosa L. Genetic control of grain quality in bread wheat (Triticum aestivum L.) grown under a range of environmental conditions. [Thesis]. University of Adelaide; 2013. Available from: http://hdl.handle.net/2440/82085

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

6. Bonneau, Julien. Genetic analysis of a region associated with heat and drought tolerance on chromosome 3B of hexaploid wheat (Triticum aestivum).

Degree: 2013, University of Adelaide

URL: http://hdl.handle.net/2440/96168

► Drought and heat can occur during the growth cycle of crops and severely reduce yield. A QTL associated with yield and yield-related component was found… (more)

▼ Drought and heat can occur during the growth cycle of crops and severely reduce yield. A QTL associated with yield and yield-related component was found in four wheat populations (Triticum aestivum L.) on the long arm of chromosome 3B “qYDH.3BL”. The four populations were grown under various climatic conditions including drought, heat and combinations of both in a number of different areas (Australia and Mexico). Linear mixed models that partition and account for genetic and non-genetic or extraneous variation were used to detect loci in single-environment and/or multi-environment QTL analysis using ASReml-R. The alleles carried by RAC875, Excalibur or Drysdale improved grain yield by between 5% and 12.5%. Two doubled haploid populations (RAC875/Kukri and Excalibur/Kukri) and two recombinant inbred line populations (RAC875/Kukri and Gladius/Drysdale) were used to fine map qYDH.3BL and identify candidate gene(s). A total of thirty-seven molecular markers were mapped on one or both genetic maps of chromosome 3B enabling development of a consensus genetic map of the qYDH.3BL region. The markers were selected based on comparisons with a published “neighbour map” of chromosome 3B or designed using either BAC-end, contig or gene sequences from the chromosome 3B sequencing project; 3BSEQ (http://urgi.versailles.inra.fr/) (cv. Chinese Spring). A positional cloning approach was used to identify candidate genes for qYDH.3BL. Molecular markers from the targeted region were assigned to physical contigs by screening the chromosome 3B BAC library experimentally using PCR or in silico by sequence comparison. A total of eight physical contigs containing 85 genes, were anchored to the qYDH.3BL region. Public and in-house resources of wheat transcript sequences were used to restrict the gene list to 65 expressed genes. Based on comparison of the 65 gene sequences to gene probes in a drought transcriptomic database, three genes were found to be differentially expressed between RAC875 and Kukri under drought conditions. Short genomic sequence reads (10× coverage) from each of the five parental lines (RAC875, Kukri, Excalibur, Gladius and Drysdale) were mapped against the 65 genes for polymorphism discovery. One gene exhibited sequence polymorphism between the drought tolerant parents (RAC875, Excalibur and Drysdale) and the drought-sensitive parents (Gladius and Kukri). In addition, presence/absence polymorphisms were consistently detected throughout a region containing 12 genes, indicating that the drought tolerant parents may have a deletion (or alien introgression) in this region. Thus, in this work, we confirmed the genetic effect of qYDH.3BL in multiple environments and multiple populations, saturated the target region with new molecular markers and defined a preliminary list of genes located in the qYDH.3BL region and selected candidate genes for further investigations. Advisors/Committee Members: Langridge, Peter (advisor), Feuillet, Catherine (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: bread wheat; multi-environment; chromosome 3B; molecular marker; positional cloning

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bonneau, J. (2013). Genetic analysis of a region associated with heat and drought tolerance on chromosome 3B of hexaploid wheat (Triticum aestivum). (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/96168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bonneau, Julien. “Genetic analysis of a region associated with heat and drought tolerance on chromosome 3B of hexaploid wheat (Triticum aestivum).” 2013. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/96168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bonneau, Julien. “Genetic analysis of a region associated with heat and drought tolerance on chromosome 3B of hexaploid wheat (Triticum aestivum).” 2013. Web. 15 Apr 2021.

Vancouver:

Bonneau J. Genetic analysis of a region associated with heat and drought tolerance on chromosome 3B of hexaploid wheat (Triticum aestivum). [Internet] [Thesis]. University of Adelaide; 2013. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/96168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bonneau J. Genetic analysis of a region associated with heat and drought tolerance on chromosome 3B of hexaploid wheat (Triticum aestivum). [Thesis]. University of Adelaide; 2013. Available from: http://hdl.handle.net/2440/96168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Mahjourimajd, Saba. Dissecting genetic variation for nitrogen use efficiency in wheat.

Degree: 2015, University of Adelaide

URL: http://hdl.handle.net/2440/103589

► Nitrogen (N) is essential for high grain yield (GY) in cereals. A major aim of breeding programs is to increase GY while minimising the level… (more)

▼ Nitrogen (N) is essential for high grain yield (GY) in cereals. A major aim of breeding programs is to increase GY while minimising the level of external inputs, such as N fertilisation. Nitrogen Use Efficiency (NUE) is a complex trait controlled by both genetic and environmental factors resulting in variation depending on seasonal growth conditions. Only 30-50% of N supplied is actually taken up by the plants with the extra N lost through run-off, leaching, denitrification and gas emission. These losses have a negative environmental impact, leading to surface and underground water pollution, algae blooms and intensifying global warming. In addition, nitrogen (N) application is costly further emphasising the importance of NUE improvement to reduce the economic and environmental issues associated with N application. NUE of wheat is important in all production areas but little is known about genetic variation for NUE in low-yielding environments such the Mediterranean-type climate of Southern Australia with low rainfall and high temperatures during critical growth periods. Research described in this thesis evaluated variation in NUE in Australian wheat germplasm and then to identify loci regulating NUE traits in a bi-parental mapping population of RAC875/Kukri. Improvement in NUE will require the integration of physiological and molecular aspects of N status in plants under different growth conditions: the highly variable conditions of field trials and controlled environments such as under hydroponics. The assessment of NUE and N response under both field and controlled conditions could facilitate the identification of traits and QTL and lead to the discovery of candidate genes underlying the traits. The first step of this research involved NUE traits and N response assessment of Australian cultivars in different environments, with varying N input. Genetic variation for NUE was identified in Australian spring wheat cultivars, and the cultivars were ranked for their N-efficiency and responsiveness. The dissection of genetic variation for NUE was investigated in the RAC875/Kukri population across six field trials between 2011 and 2013 covering 16 environment by treatment combinations. Nitrogen responsiveness was compared with N efficiency and the genotypes were ranked for the consistency of a positive response and high efficiency of N use versus negative responsiveness and low efficiency. Quantitative Trait Loci (QTL) analysis identified the genome regions associated with GY, grain quality and responsiveness to N. In addition, specific-environment associated N QTL were identified. A QTL on chromosome 2A was detected for most of traits studied and across multiple environments. Further stable QTL were identified on chromosomes 1A, 1B, 2A, 3D, 7A and 7B for GY across environments. The physiological response to N was studied at the early stages of growth for selected lines in a hydroponics system that allowed the measurement of N uptake and utilisation. The aim of the experiments was to investigate the physiological basis… Advisors/Committee Members: Langridge, Peter (advisor), Okamoto, Mamoru (advisor), Kuchel, Haydn (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: wheat; selection; breeding; nitrogen use efficiency (NUE); best linear unbiased prediction (BLUP); quantitative trait loci (QTL)

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APA (6^th Edition):

Mahjourimajd, S. (2015). Dissecting genetic variation for nitrogen use efficiency in wheat. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/103589

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mahjourimajd, Saba. “Dissecting genetic variation for nitrogen use efficiency in wheat.” 2015. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/103589.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mahjourimajd, Saba. “Dissecting genetic variation for nitrogen use efficiency in wheat.” 2015. Web. 15 Apr 2021.

Vancouver:

Mahjourimajd S. Dissecting genetic variation for nitrogen use efficiency in wheat. [Internet] [Thesis]. University of Adelaide; 2015. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/103589.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mahjourimajd S. Dissecting genetic variation for nitrogen use efficiency in wheat. [Thesis]. University of Adelaide; 2015. Available from: http://hdl.handle.net/2440/103589

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

8. Nagahatenna, Dilrukshi Shashikala Kumari. Investigating the role of tetrapyrrole biosynthesis under drought stress in cereal transgenics.

Degree: 2015, University of Adelaide

URL: http://hdl.handle.net/2440/104054

► The tetrapyrrole biosynthesis pathway leads to chlorophyll and heme production and plays a key role in primary physiological processes such as photosynthesis and respiration. Recent… (more)

▼ The tetrapyrrole biosynthesis pathway leads to chlorophyll and heme production and plays a key role in primary physiological processes such as photosynthesis and respiration. Recent studies have shed light on heme as a potential candidate molecule for triggering stress defence responses. However, detailed investigations are yet to be conducted to elucidate the potential role of heme in regulating responses to complex abiotic stress conditions such as drought. The terminal enzyme of heme biosynthesis is Ferrochelatase (FC), for which there are two isoforms encoded by separate genes (FC1 and FC2). Previous studies propose that the two FCs synthesize two physiologically distinct heme pools with different cellular functions. The overall scientific goal of this thesis was to investigate the roles of the two FCs in photosynthesis, drought and oxidative stress tolerance. In this study, barley (Hordeum vulgare) was used as both a major cereal crop and also as a model plant for other commercially relevant rain-fed cereal crops. Two FCs in barley (HvFC1 and HvFC2) were identified and their tissue-specific and stress-responsive expression patterns were investigated. These genes were cloned from the cultivar Golden Promise (GP) and transgenic lines ectopically overexpressing either HvFC1 or HvFC2 were generated. From 29 independent T₀ transgenic lines obtained for each FC construct, three single-copy transgenic lines ectopically overexpressing either HvFC1 or HvFC2 were evaluated for photosynthetic performance, oxidative and drought stress tolerance. The two HvFC isoforms share a common catalytic FC domain, while HvFC2 additionally contains C-terminal chlorophyll a/b binding (CAB) domain. The two genes are differentially expressed in photosynthetic and non-photosynthetic tissues and have distinct stress responsive expression profiles, implying that they may have distinct roles. Transgenic plants ectopically overexpressing either HvFC1 or HvFC2 exhibited significantly higher chlorophyll content, stomatal conductance (gs) [s subscript], carboxylation efficiency (CE) and photosynthetic rate relative to controls under both non-stressed and drought stress conditions. Furthermore, these transgenics, showed wilting avoidance and maintained higher leaf water content and water use efficiency relative to control plants when subjected to drought stress. Overexpression of HvFCs significantly up-regulated nuclear genes associated with ROS detoxification under drought stress. It also reduced photo-oxidative damage caused by perturbation of tetrapyrrole biosynthesis in tigrinaᵈ¹² mutants. Taken together, this study indicates that both HvFCs play roles in photosynthesis and improving oxidative and drought stress tolerance. The results reported in this thesis suggest that both HvFC derived heme pools are likely to be involved in chloroplast-to-nuclear retrograde signaling to trigger drought and oxidative stress tolerance. This study also highlights the tetrapyrrole pathway as an important target for engineering improved crop performance in both… Advisors/Committee Members: Langridge, Peter (advisor), Whitford, Ryan (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: barley; tetrapyrrole; heme; ferrochelatase; chlorophyll; drought stress; photosynthesis; photo-oxidation; transcriptional regulation; Research by Publication

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nagahatenna, D. S. K. (2015). Investigating the role of tetrapyrrole biosynthesis under drought stress in cereal transgenics. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/104054

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nagahatenna, Dilrukshi Shashikala Kumari. “Investigating the role of tetrapyrrole biosynthesis under drought stress in cereal transgenics.” 2015. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/104054.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nagahatenna, Dilrukshi Shashikala Kumari. “Investigating the role of tetrapyrrole biosynthesis under drought stress in cereal transgenics.” 2015. Web. 15 Apr 2021.

Vancouver:

Nagahatenna DSK. Investigating the role of tetrapyrrole biosynthesis under drought stress in cereal transgenics. [Internet] [Thesis]. University of Adelaide; 2015. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/104054.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nagahatenna DSK. Investigating the role of tetrapyrrole biosynthesis under drought stress in cereal transgenics. [Thesis]. University of Adelaide; 2015. Available from: http://hdl.handle.net/2440/104054

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

9. Rahimi Eichi, Vahid. Understanding the interactions between biomass, grain yield and grain protein content in low and high protein wheat cultivars.

Degree: 2020, University of Adelaide

URL: http://hdl.handle.net/2440/129088

► Grain protein content (GPC) is a key quality attribute and an important marketing trait in wheat. However, a negative relationship between grain yield and GPC… (more)

▼ Grain protein content (GPC) is a key quality attribute and an important marketing trait in wheat. However, a negative relationship between grain yield and GPC has limited selection for increased GPC, since grain yield is the primary driver of breeding programs. GPC is strongly influenced by nitrogen (N) fertilizer application, but the N-use efficiency (NUE) of high and low GPC genotypes appears to be genetically determined. The aim of this PhD thesis was to investigate the grain yield-GPC relationship under controlled and field conditions, and to suggest selection targets and traits for improving NUE in wheat. Firstly, the N responsiveness of six wheat genotypes that varied in GPC were examined under controlled condition. This experiment was designed around non-destructive estimation of biomass using a high-throughput image-based phenotyping system. In parallel, field trials were conducted to allow the comparison of results obtained from the controlled condition study using the six selected genotypes. Estimating the rate of biomass accumulation in breeding plots in the field is difficult. Therefore, the growth rate of biomass related traits such as height and ground cover were assessed in these trials. To examine the grain yield- GPC relationship under multi-environmental conditions, the grain yield and GPC data of over 200 wheat genotypes obtained from the Australian National Variety Trials (NVT) across the Australian wheat-belt were analysed. Results of the controlled environment experiment showed that high GPC genotypes appeared to demand more N to grow their biomass. In both controlled and field environments, high GPC genotypes slowed down the rate of biomass growth under low N supply. Under low yielding conditions, high GPC genotypes seemed able to manage grain N reserves by compromising biomass production. These results indicated the importance of biomass growth analysis to show the differences in the N responsiveness of high and low GPC genotypes. Differences between high and low GPC genotypes in responding to low N could be due to their history of selection. N effect is strongly associated with the amount of available water in the soil. Controlled and multi-environmental studies showed that the slope of the relationship between grain yield and GPC is steeper in low compared to high yielding environments. Therefore, high GPC genotypes bred under stress conditions sacrifice yield in favour of GPC, possibly to enhance the survival chance by producing fewer grains with sufficient nutrient levels. Conversely, low GPC genotypes bred in high yielding environment are less conservative compared to high GPC genotypes in using N for yield production. The outcomes of this PhD project highlight the importance of considering environmental factors for improving NUE in breeding programs. It recommends that wheat breeders focus on selecting in low yielding environments for high yield and high GPC genotypes. Advisors/Committee Members: Langridge, Peter (advisor), Garnett, Trevor (advisor), Okamoto, Mamoru (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: Grain protein content; grain yield; biomass; non-destructive measurements; controlled and field conditions; multi-environment; National Variety Trials; high and low protein wheat; grain protein deviation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rahimi Eichi, V. (2020). Understanding the interactions between biomass, grain yield and grain protein content in low and high protein wheat cultivars. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/129088

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rahimi Eichi, Vahid. “Understanding the interactions between biomass, grain yield and grain protein content in low and high protein wheat cultivars.” 2020. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/129088.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rahimi Eichi, Vahid. “Understanding the interactions between biomass, grain yield and grain protein content in low and high protein wheat cultivars.” 2020. Web. 15 Apr 2021.

Vancouver:

Rahimi Eichi V. Understanding the interactions between biomass, grain yield and grain protein content in low and high protein wheat cultivars. [Internet] [Thesis]. University of Adelaide; 2020. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/129088.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rahimi Eichi V. Understanding the interactions between biomass, grain yield and grain protein content in low and high protein wheat cultivars. [Thesis]. University of Adelaide; 2020. Available from: http://hdl.handle.net/2440/129088

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

10. Thomelin, Pauline. Genes, haplotypes and physiological traits associated with a chromosome 3B locus for wheat improvement in hot climates.

Degree: 2018, University of Adelaide

URL: http://hdl.handle.net/2440/118118

► Crop productivity in many wheat cultivation areas is severely affected by dry and hot conditions, increasing the yield gap between potential and actual yield. To… (more)

▼ Crop productivity in many wheat cultivation areas is severely affected by dry and hot conditions, increasing the yield gap between potential and actual yield. To close this gap, the identification of genes contributing to yield in stressed conditions is one key to the breeding of tolerant cultivars. However, due to low stability across environments, no genes have yet been identified for yield variation. In this project, we focussed on the positional cloning of a quantitative trait locus (QTL), qYDH.3BL, located on the wheat chromosome arm 3BL. The QTL has been identified by the multi-environment analysis of the double-haploid (DH) population of the cross from the drought tolerant line RAC875 and the susceptible variety Kukri. The QTL was constitutively expressed in the Mexican environment with the positive allele from RAC875 associated with an increase in yield, thousand grain weight and early vigour under dry and hot conditions. Greater allele effect dependent on temperature has been observed at qYDH.3BL, suggesting that the QTL is heat related. To fine map qYDH.3BL, we developed a deep-soil platform using 1 m deep wheelie bins placed in the polytunnel to increase temperature. We confirmed the expression of qYDH.3BL using a set of RAC875 x Kukri RILs. Single marker analysis showed the positive allele from RAC875 associated with spike length and biomass, early vigour and stem biomass. The development of a high-density genetic map of qYDH.3BL in RAC875, combined with the deep-platform experiment, narrowed the QTL interval to a 690 kbp sequence. The anchoring of the interval in the physical assembly of the wheat cv. Chinese Spring reference genome (IWGSC Ref 1.0) identified 12 candidate genes. The study of the allelic diversity at qYDH.3BL in a wheat diversity panel of 808 accessions identified four haplotypes. Haplotype I, the RAC875 allele was over-represented in the CIMMYT germplasm suggesting that the allele may have been selected for yield in a Mexican environment-type. To study the physiological mechanisms under qYDH.3BL control, we developed heterozygous inbred families (HIF). The lines were phenotyped in the deep soil platform. The lines with the RAC875 allele among the HIFs increased biomass, single grain weight and number of spikelets per spike. Water use was also measured in the deep soil platform using sap flow sensors. RAC875 had an increased water use compared to Kukri. A similar pattern was observed in the HIFs, the lines with the RAC875 allele had a higher water use compared to those with the Kukri allele. As qYDH.3BL was constitutively expressed in the Mexican environments characterised by a deep soil and RAC875 was shown to have a lower root conductivity than Kukri due to its root anatomy, we also phenotyped the roots of the HIFs. We did not identify root traits that could contribute to the heat tolerance mechanism associated with qYDH.3BL. Finally, we studied the expression of the 12 candidate genes within the 690 kbp interval in the HIFs. Expression analysis identified a strong candidate gene, Seven… Advisors/Committee Members: Fleury, Delphine (advisor), Tricker, Penny (advisor), Langridge, Peter (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: Triticum aestivum; positional cloning; quantitative trait locus (QTL); drought; heat stress; heterozygous inbred families (HIFs)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thomelin, P. (2018). Genes, haplotypes and physiological traits associated with a chromosome 3B locus for wheat improvement in hot climates. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/118118

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thomelin, Pauline. “Genes, haplotypes and physiological traits associated with a chromosome 3B locus for wheat improvement in hot climates.” 2018. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/118118.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thomelin, Pauline. “Genes, haplotypes and physiological traits associated with a chromosome 3B locus for wheat improvement in hot climates.” 2018. Web. 15 Apr 2021.

Vancouver:

Thomelin P. Genes, haplotypes and physiological traits associated with a chromosome 3B locus for wheat improvement in hot climates. [Internet] [Thesis]. University of Adelaide; 2018. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/118118.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thomelin P. Genes, haplotypes and physiological traits associated with a chromosome 3B locus for wheat improvement in hot climates. [Thesis]. University of Adelaide; 2018. Available from: http://hdl.handle.net/2440/118118

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

11. Li, Ming. Identification and expression analyses of genes involved in early endosperm development in Arabidopsis and cereals.

Degree: 2011, University of Adelaide

URL: http://hdl.handle.net/2440/69213

► Cereal seeds are important source for food, feed and also provide an industrial raw material. A sound understanding of seed initiation and the control of… (more)

▼ Cereal seeds are important source for food, feed and also provide an industrial raw material. A sound understanding of seed initiation and the control of early endosperm development will be important for increasing cereal crop yield and improving grain quality using biotechnology. The approach taken in this project was to investigate the potential application of candidate genes and pathways for manipulating endosperm initiation and development. The candidate system chosen was the FIS class Polycomb group complex since there has been considerable work on the role of this complex in the transcriptional regulation of seed initiation and endosperm cellularization in Arabidopsis. The aims of the work described in this thesis were, first to identify and characterize novel proteins which directly interact with the known PcG members FIE and SWN. These two proteins play a key role in the regulation of endosperm proliferation in Arabidopsis. The second aim was to identify homologs from rice and Arabidopsis which potentially regulate grain size, weight and composition and to investigate the expression profiles and possible functions of these genes and isolate endosperm-specific promoters. Several novel plant PcG complex proteins from Arabidopsis were identified via yeast two-hybrid (Y2H) screens. Several other interacting transcription factors of FIE were identified, including SWN (a.k.a. EZA1/SWINGER), AREB2 (ABA-responsive element binding factor 2), VPL (Viviparous-like) and ICE2 (Inducer of CBF Expression 2). Interacting partners of SWN were also isolated. These included AtRING-H2, an E3 type protein ubiquitin ligase, v-SNARE, and BrD (Bromodomain-containing protein), and a plant PHD finger-containing factor. The expression profile of these genes was studied using quantitative real-time PCR (qRT-PCR) in a series of Arabidopsis tissues prepared from wild type (WT) plants. Expression of these genes was also studied by using the predicted 5’-regulatory sequences of SWN, AtRING-H2, AREB2, VPL and ICE2 fused to GUS reporter gene and stably transformed into Arabidopsis. The expression pattern of each candidate was revealed by GUS assays. Results showed that the VPL promoter was specific to the male gamete, while the AtRING-H2 promoter was found to be male gametophyte- and seed-specific. In order to elucidate the functions of the key PcG partner SWN and its possible role in the regulation of seed initiation, T-DNA insertion lines were analyzed and gene knockdown constructs were developed. Seed development was disrupted by 50% in one SWN silencing line via dsRNAi. A preliminary study of the SWN silencing plant was carried out but further characterization is needed to uncover the role of SWN in seed development. Manipulation of endosperm development will be dependent on access to promoters specific to early endosperm development. Arabidopsis END1, a homolog of a barley endosperm marker gene was characterized in this project. AtEND1 was identified as a member from Arabidopsis lipid transfer protein family. This gene was specifically… Advisors/Committee Members: Langridge, Peter (advisor), Lopato, Sergiy (advisor), Koltunow, Anna Maria Grazyna (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: endosperm; Arabidopsis; cereals; promoter

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, M. (2011). Identification and expression analyses of genes involved in early endosperm development in Arabidopsis and cereals. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/69213

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Ming. “Identification and expression analyses of genes involved in early endosperm development in Arabidopsis and cereals.” 2011. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/69213.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Ming. “Identification and expression analyses of genes involved in early endosperm development in Arabidopsis and cereals.” 2011. Web. 15 Apr 2021.

Vancouver:

Li M. Identification and expression analyses of genes involved in early endosperm development in Arabidopsis and cereals. [Internet] [Thesis]. University of Adelaide; 2011. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/69213.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li M. Identification and expression analyses of genes involved in early endosperm development in Arabidopsis and cereals. [Thesis]. University of Adelaide; 2011. Available from: http://hdl.handle.net/2440/69213

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

12. Shamaya, Nawar Jalal. Genetic studies of salinity tolerance in wheat.

Degree: 2014, University of Adelaide

URL: http://hdl.handle.net/2440/92806

► Salinity is an important issue in arid and semi-arid regions of the world, both in irrigated and dryland agriculture. Increasing salinity tolerance of crops is… (more)

▼ Salinity is an important issue in arid and semi-arid regions of the world, both in irrigated and dryland agriculture. Increasing salinity tolerance of crops is a feasible approach to tackling salinity. Focusing on the physiological traits associated with salinity tolerance such as Na⁺ exclusion and osmotic stress tolerance simplifies the strategies for improving tolerance. The first aim of the study described in this thesis was the development of a high throughput technique for the measurement of osmotic stress tolerance in bread wheat. This technique was then applied to 162 recombinant inbred lines derived from crossing two Australian bread wheat cultivars (Gladius and Drysdale), to identify the loci associated with osmotic stress tolerance and 4th leaf Na⁺ accumulation. This population was grown under two growth conditions – a pot-soil set-up with non-destructive imaging system (LemnaTec Scanalyzer 3D technology) for the estimation of osmotic stress tolerance using high through-put system (conveyor belt system) and a supported hydroponics set-up for 4th leaf Na⁺ and 4th leaf K⁺ accumulation measurements. In the soil based study, QTL analyses revealed two major QTL on the distal regions of the short arms of chromosomes 2B and 1B, where the salinity tolerance index (shoot biomass in saline conditions relative to shoot biomass in control conditions) and osmotic stress tolerance overlapped. Another significant QTL for osmotic stress tolerance was mapped onto the distal region of the long arm of chromosome 5D. In the hydroponics study, two QTL associated with 4th leaf Na⁺ accumulation were mapped to the distal regions of the long arms of chromosomes 1D and 3B. Loci containing a vernalisation gene (VRN-A1), on the long arm of chromosome 5A, and a photoperiod gene (Ppd-D1), on the short arm of chromosome 2D, had an impact on tiller number, shoot biomass and shoot water content in salt and control conditions. The second aim of the research program was to study the genetics of Na⁺ exclusion in two Afghani durum wheat landraces, which accumulated half the amount of 3rd leaf Na⁺ compared to Australian commercial durum wheat cultivars. These landraces were crossed with an Australian durum wheat (cv Jandaroi) and F₂ populations were developed. The parents and F₂ population were grown in a supported hydroponics system at 100 mM NaCl, and the Na⁺+ and K⁺ concentrations in the third leaf was measured after ten days growth in salt. Selective genotyping analysis using DArT markers and bulked segregant analysis (BSA) using SNP markers were carried out to detect the putative genomic regions responsible for salinity tolerance. Both analyses revealed a locus on the distal region of the long arm of chromosome 4B associated with Na⁺ and K⁺ accumulation and the ratio of K⁺/Na⁺ in the third leaf; the favourable allele derived from the Afghani landraces. BSA identified another locus on the distal region of the long arm of chromosome 3B, associated only with 3rd leaf Na⁺ accumulation and the favourable allele was inherited from Jandaroi.… Advisors/Committee Members: Tester, Mark Alfred (advisor), Shavrukov, Yuri (advisor), Langridge, Peter (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: salinity tolerance; osmotic stress tolerance; Na+ exclusion; QTL analysis; Bread wheat; Durum wheat

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shamaya, N. J. (2014). Genetic studies of salinity tolerance in wheat. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/92806

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shamaya, Nawar Jalal. “Genetic studies of salinity tolerance in wheat.” 2014. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/92806.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shamaya, Nawar Jalal. “Genetic studies of salinity tolerance in wheat.” 2014. Web. 15 Apr 2021.

Vancouver:

Shamaya NJ. Genetic studies of salinity tolerance in wheat. [Internet] [Thesis]. University of Adelaide; 2014. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/92806.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shamaya NJ. Genetic studies of salinity tolerance in wheat. [Thesis]. University of Adelaide; 2014. Available from: http://hdl.handle.net/2440/92806

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

13. Pillman, Katherine. Transcription factors involved in cold response in plants.

Degree: 2010, University of Adelaide

URL: http://hdl.handle.net/2440/63421

► Studies in Arabidopsis have shown cold stress tolerance can be enhanced by manipulation of the CBF/DREB and ICE transcription factor genes. To date, few studies… (more)

▼ Studies in Arabidopsis have shown cold stress tolerance can be enhanced by manipulation of the CBF/DREB and ICE transcription factor genes. To date, few studies have investigated CBF and ICE genes in crops species such as barley. Using a C-repeat element as bait, two CBF genes were isolated from a cold-stressed barley cDNA library, HvCBF16 and HvCBF23. HvCBF16 was induced by cold treatment but not by other abiotic stresses. HvCBF23 was constitutively expressed and was not induced by cold treatment. The analysis of transgenic plants expressing these genes will determine their importance in cold tolerance. Transgenic barley plants expressing the barley gene HvCBF2A were found to be more cold tolerant in controlled temperature trials, and hence were assayed to determine the basis of their acquired phenotype. Northern and qRT-PCR analysis showed that four genes known to be involved in cold tolerance were significantly upregulated. Importantly the increased expression was proportional to the level of transgene expression and levels were higher following cold treatment. A homolog of the Arabidopsis ICE transcription factor was isolated from a freezing-tolerant barley variety (Hordeum vulgare L. cv. Haruna Nijo) and transcript analysis of HvICE2 under various abiotic stresses showed that expression of HvICE2 was induced at low temperatures, particularly in floral tissues. HvICE2 was over-expressed using the maize ubiquitin constitutive promoter in transgenic barley. Expression analysis of putative downstream genes, including various COR genes, in the transgenic plants before and during cold treatment did not reveal any alteration in expression. This suggests HvICE2 that the COR genes studied are not targets of HvICE2 or that additional factors or conditions are required for effective function of HvICE2. Transgenic Arabidopsis plants were produced with over- or reduced-expression of the uncharacterised ICE gene, AtICE2. The cold tolerance of the AtICE2 transgenic lines was not significantly different from wild type plants. Advisors/Committee Members: Jacobs, Andrew Keith (advisor), Lopato, Sergiy (advisor), Langridge, Peter (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: transcription factor; CBF; ICE; barley; Arabidopsis; plant; transgenic; signalling; cold; freezing

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APA (6^th Edition):

Pillman, K. (2010). Transcription factors involved in cold response in plants. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/63421

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pillman, Katherine. “Transcription factors involved in cold response in plants.” 2010. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/63421.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pillman, Katherine. “Transcription factors involved in cold response in plants.” 2010. Web. 15 Apr 2021.

Vancouver:

Pillman K. Transcription factors involved in cold response in plants. [Internet] [Thesis]. University of Adelaide; 2010. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/63421.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pillman K. Transcription factors involved in cold response in plants. [Thesis]. University of Adelaide; 2010. Available from: http://hdl.handle.net/2440/63421

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Atieno, Judith Akinyi. Unravelling the physiology and genetics of salinity tolerance in chickpea (Cicer arietinum L.).

Degree: 2017, University of Adelaide

URL: http://hdl.handle.net/2440/113320

► Chickpea (Cicer arietinum L.) is a nutritious legume predominantly grown in semi-arid environments under rain fed conditions, but is highly sensitive to soil salinity. Until… (more)

▼ Chickpea (Cicer arietinum L.) is a nutritious legume predominantly grown in semi-arid environments under rain fed conditions, but is highly sensitive to soil salinity. Until recently, there has been slow progress in the application of molecular genetics in chickpea breeding. This is primarily because the available genetic variation in international chickpea germplasm collections has not been extensively characterised due to a lack of available genomics tools and high-throughput phenotyping resources. Molecular genetic approaches are needed to identify key loci with the potential to improve salinity tolerance in chickpea. In this project, genetic analysis was conducted on two populations: A recombinant inbred line (RIL) population of 200 individuals developed from a cross between Genesis836 and Rupali which are known to contrast in their tolerance to salinity and a diversity panel consisting of 245 chickpea accessions of diverse genetic background from ICRISAT. For phenotyping, an image-based high-throughput phenotyping platform was used. Data on growth rate, water use, plant senescence and necrosis, and agronomic traits were collected under both control and saline conditions (40 mM for diversity panel and 70 mM NaCl for RIL). In depth studies including differential metabolite accumulation and senescence detection were carried out to increase our understanding of the response of chickpea to salinity. Genesis836 and Rupali differentially accumulated metabolites associated with the TCA cycle, carbon and amino acid metabolism. Higher senescence scores were recorded in Rupali compared to Genesis836. On average, salinity reduced plant growth rate by 20%, plant height by 15% and shoot biomass by 28%. Additionally, salinity induced pod abortion and inhibited pod filling, which consequently reduced seed number and seed yield by 16% and 32%, respectively. Path analysis was utilised to understand the intricate relationship existing between the traits measured and aided in the identification of those most related to salinity tolerance. This analysis showed that seed number under salt was highly related to salinity tolerance in chickpea. To identify Quantitative Trait Loci (QTL) underlying salinity tolerance in chickpea, two complimentary genetic analysis approaches were used: genome-wide association studies (GWAS) and linkage mapping. Phenotypic data was combined with genotypic data from both the diversity panel (generated through whole-genome resequencing) and RIL population (from DArTseq). Linkage mapping and GWAS identified a total of 57 QTL and 54 marker-trait associations (MTAs), respectively. The loci identified were linked to growth rate, yield, yield components and ion accumulation. A novel major QTL for relative growth rate on chromosome 4 that explained 42.6% of genetic variation, was identified by both genetic analyses. This QTL co-located with several other QTL identified, including those associated with projected shoot area, water use, 100-seed weight, the number of filled pods, harvest index, seed number and seed… Advisors/Committee Members: Sutton, Tim (advisor), Langridge, Peter (advisor), Li, Yongle (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: chickpea; salinity tolerance; linkage mapping; GWAS; QTL; Research by Publication

…Functional Genomics and University of Adelaide. viii List of publications 1. Dias D.A, Hill C.B…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Atieno, J. A. (2017). Unravelling the physiology and genetics of salinity tolerance in chickpea (Cicer arietinum L.). (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/113320

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Atieno, Judith Akinyi. “Unravelling the physiology and genetics of salinity tolerance in chickpea (Cicer arietinum L.).” 2017. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/113320.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Atieno, Judith Akinyi. “Unravelling the physiology and genetics of salinity tolerance in chickpea (Cicer arietinum L.).” 2017. Web. 15 Apr 2021.

Vancouver:

Atieno JA. Unravelling the physiology and genetics of salinity tolerance in chickpea (Cicer arietinum L.). [Internet] [Thesis]. University of Adelaide; 2017. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/113320.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Atieno JA. Unravelling the physiology and genetics of salinity tolerance in chickpea (Cicer arietinum L.). [Thesis]. University of Adelaide; 2017. Available from: http://hdl.handle.net/2440/113320

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Bennett, Dion. A genetic dissection of drought and heat tolerance related traits in bread wheat (Triticum aestivum L.).

Degree: 2013, University of Adelaide

URL: http://hdl.handle.net/2440/83641

► This study was conducted with the aim of improving our understanding of the genetic basis of the superior grain yield of an elite bread wheat… (more)

▼ This study was conducted with the aim of improving our understanding of the genetic basis of the superior grain yield of an elite bread wheat breeding line, RAC875, under drought and heat stressed Mediterranean-type climates in southern Australia. Here, these abiotic stresses present a significant barrier to production. Kukri is a locally adapted variety which achieves acceptable grain yield under more favourable conditions, but relatively low grain yields under severe stress. A cross between the two lines resulted in an F₁ derived doubled haploid population consisting of 368 individuals. The population was initially used for the genetic dissection of time to ear emergence and flag leaf glaucousness, with the latter trait hypothesised to explain a significant proportion of RAC875’s relative drought and heat tolerance. Whilst parents of the population achieved similar time to ear emergence, segregation for Ppd-B1 and Ppd-D1a created large variation for this trait within the population. Two novel minor loci were detected for time to ear emergence (Q.Eet.aww-1A and Q.Zad.aww-4A), in addition to another eight known, minor loci. Five novel loci were detected for flag leaf glaucousness (Q.W.aww-3A, Q.W.aww-3B, Q.W.aww-3D, Q.W.aww-4D and Q.W.aww-5B), with one in particular (Q.W.aww-3A) accounting for up to 52 percent of the genetic variance for this trait. Sixteen field experiments were sown across southern Australia between 2006 and 2010, where average site grain yields ranged from 314 to 5275 kg ha⁻¹. Kernels per square metre was the trait most correlated with grain yield, while spikelet fertility, which had a significant positive correlation with grains per square metre in all experiments and the subsequently derived environment clusters, was also related to grain yield. Nine loci were detected for grain yield independent of time to ear emergence and plant height. Five of these loci co-located with loci for kernels per square metre and only one of these nine loci were associated with any of the loci for flag leaf glaucousness and this genetic effect was opposite (i.e. Kukri allele resulting in large glaucousness value and lower grain yield). The RAC875 allele at QTL on chromosomes 1B and 7A (Q.Yld.aww-1B and Q.Yld.aww-7A-2) was associated with greater grain yield, kernels per spikelet and kernels per square metre. These two loci were detected in environment clusters where heat stress was a differentiating factor and it was concluded that these may therefore be associated with heat stress tolerance. Another QTL of large effect was consistently detected on chromosome 6A (Q.Tkw.aww-6A), with the RAC875 allele positively affecting grain size, flag leaf width and stem water soluble carbohydrate content but resulting in lower kernels per spikelet and therefore kernels per square metre. Experiments were also sown to assess the performance of the population in north-west Mexico under well watered, high yield potential conditions, as well as drip irrigated drought treatment and late planted but well watered conditions to expose… Advisors/Committee Members: Schnurbusch, Thorsten (advisor), Kuchel, Haydn (advisor), Langridge, Peter (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: wheat; drought; heat stress; QTL

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APA (6^th Edition):

Bennett, D. (2013). A genetic dissection of drought and heat tolerance related traits in bread wheat (Triticum aestivum L.). (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/83641

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bennett, Dion. “A genetic dissection of drought and heat tolerance related traits in bread wheat (Triticum aestivum L.).” 2013. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/83641.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bennett, Dion. “A genetic dissection of drought and heat tolerance related traits in bread wheat (Triticum aestivum L.).” 2013. Web. 15 Apr 2021.

Vancouver:

Bennett D. A genetic dissection of drought and heat tolerance related traits in bread wheat (Triticum aestivum L.). [Internet] [Thesis]. University of Adelaide; 2013. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/83641.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bennett D. A genetic dissection of drought and heat tolerance related traits in bread wheat (Triticum aestivum L.). [Thesis]. University of Adelaide; 2013. Available from: http://hdl.handle.net/2440/83641

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Arndell, Taj. Genome editing in wheat with CRISPR/Cas9.

Degree: 2019, University of Adelaide

URL: http://hdl.handle.net/2440/120690

► Genetically engineered crops have the potential to play a key role in achieving global food security and transitioning to a more sustainable agriculture. In recent… (more)

▼ Genetically engineered crops have the potential to play a key role in achieving global food security and transitioning to a more sustainable agriculture. In recent years, the CRISPR/Cas9 system has emerged as a powerful tool for crop genome editing. CRISPR/Cas9 enables the targeted and precise modification of plant genomes via the creation and subsequent repair of site-specific DNA double-strand breaks (DSBs). The system consists of the Cas9 endonuclease in complex with a small guide RNA (gRNA) that is designed to target a specific site in the genome. Site-specific DSBs generated by Cas9 are repaired through non-homologous end joining (NHEJ) or homology directed repair (HDR). NHEJ is error-prone and often produces small insertions or deletions (indels) that result in gene knockout. Alternatively, if an exogenous DNA donor template is delivered to the cell, then precise modifications can be made via HDR. The CRISPR/Cas9 system has been successfully applied to many model and crop plants. However, it can be difficult to achieve highly efficient and specific editing in polyploid species. Therefore, the main aim of this PhD project was to develop tools and methods for optimising the CRISPR/Cas9 for efficient and specific genome editing in hexaploid bread wheat (Triticum aestivum). To test the efficacy of the CRISPR/Cas9 system for gene knockout, three gRNAs were designed to target Ms1, a male fertility gene that has been proposed for use in hybrid seed production. CRISPR/Cas9 vectors were delivered to immature embryos via Agrobacterium-mediated stable transformation, and the regenerated T0 lines were screened for targeted indels produced via NHEJ. Only one of the three gRNAs was efficacious. Five per cent (2/40) of T0 lines carrying the active gRNA were edited and male sterile, whereas all unedited lines were fully fertile. The recessive mutations were stably transmitted to the T1, T2 and T3 generations, as was the male sterile phonotype. Given the observed variability in the efficacy of different gRNAs targeting the same gene, and given that wheat transformation and tissue culture takes months and is laborious, a method was developed for the rapid assessment of gRNA activity and specificity. Seven gRNAs were designed to target EPSPS, a gene involved in aromatic amino acid biosynthesis. CRISPR/Cas9 vectors were then transiently transformed into wheat protoplasts. Three out of the seven gRNAs induced mutations at moderate to high frequencies. gRNA specificity was correlated with the number and distribution of mismatches in the ‘seed’ region of the gRNA. One of the gRNAs was selected as potentially suitable for the development of non-transgenic herbicide resistant wheat lines. Advisors/Committee Members: Whitford, Ryan (advisor), Langridge, Peter (advisor), Baumann, Ute (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: CRISPR/Cas9; genome editing; plant genome editing; plant genetic engineering; wheat; Triticum aestivum; hybrid breeding; protoplast

…people at the University of Adelaide who helped me during my PhD: Dr. Larissa Chirkova, Dr… …friends at The University of Adelaide. You know who you are. I am grateful to the University of… …agreement with The University of Adelaide Higher Degree by Research Specifications for Thesis 2018… …University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia 20 Summary The CRISPR/Cas9… …Adelaide for supporting me financially during my PhD. Finally, I would like to thank my family…

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APA (6^th Edition):

Arndell, T. (2019). Genome editing in wheat with CRISPR/Cas9. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/120690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Arndell, Taj. “Genome editing in wheat with CRISPR/Cas9.” 2019. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/120690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Arndell, Taj. “Genome editing in wheat with CRISPR/Cas9.” 2019. Web. 15 Apr 2021.

Vancouver:

Arndell T. Genome editing in wheat with CRISPR/Cas9. [Internet] [Thesis]. University of Adelaide; 2019. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/120690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Arndell T. Genome editing in wheat with CRISPR/Cas9. [Thesis]. University of Adelaide; 2019. Available from: http://hdl.handle.net/2440/120690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

17. Kruger, Sherri Anne. Linkage disequilibrium analysis of hexaploid wheat (Triticum aestivum L.).

Degree: 2007, University of Adelaide

URL: http://hdl.handle.net/2440/101499

► There has recently been a renewed interest in using a whole-genome approach for identifying regions with relatively small effect on a particular trait of interest.… (more)

Subjects/Keywords: wheat; Triticum aestivum L.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kruger, S. A. (2007). Linkage disequilibrium analysis of hexaploid wheat (Triticum aestivum L.). (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/101499

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kruger, Sherri Anne. “Linkage disequilibrium analysis of hexaploid wheat (Triticum aestivum L.).” 2007. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/101499.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kruger, Sherri Anne. “Linkage disequilibrium analysis of hexaploid wheat (Triticum aestivum L.).” 2007. Web. 15 Apr 2021.

Vancouver:

Kruger SA. Linkage disequilibrium analysis of hexaploid wheat (Triticum aestivum L.). [Internet] [Thesis]. University of Adelaide; 2007. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/101499.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kruger SA. Linkage disequilibrium analysis of hexaploid wheat (Triticum aestivum L.). [Thesis]. University of Adelaide; 2007. Available from: http://hdl.handle.net/2440/101499

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

18. Sutton, Timothy J. Genomics approach to investigate the molecular control of meiosis in Triticum aestivum.

Degree: 2003, University of Adelaide

URL: http://hdl.handle.net/2440/37762

► Meiosis is a cell division process central to the life cycle of all sexual eukaryotic organisms. Chromosome pairing, genetic recombination and subsequent nuclear division during… (more)

▼ Meiosis is a cell division process central to the life cycle of all sexual eukaryotic organisms. Chromosome pairing, genetic recombination and subsequent nuclear division during meiosis produces four genetically distinct haploid gametes from a single diploid cell. Allohexaploid wheat ( Triticum aestivum ) behaves meiotically as a diploid, despite the existence in the genome of three closely related ( homoeologous ) genomes, A, B and D. Chromosome pairing during prophase I of meiosis in wheat is restricted to true homologous chromosomes, the result being the formation of 21 bivalents at meiotic metaphase I. The genetic control of chromosome pairing in wheat is under the control of several pairing homoeologous ( Ph ) genes, located predominantly on chromosome groups 3 and 5. The major suppressors of homoeologous pairing are Ph1 and Ph2. Their cytogenetic effect has been intensively studied but at the molecular level little is known about their function. The isolation and characterisation of Ph genes from wheat would lead to greater understanding of chromosome pairing mechanisms in complex allopolyploids, and may enable development of effective strategies for alien gene introgression from related species to modern wheat cultivars. In this study, several genomics - based approaches were adopted to explore the expressed portion of the wheat genome in order to identify and characterise genes that could function in the molecular processes regulating meiosis. The first approach used comparative genetics to characterise the region deleted in the ph2a mutant ( a deletion mutant at Ph2 ). The rice genomic region syntenous to that deleted in the ph2a mutant was identified through comparative mapping and used in searches of wheat databases to identify ESTs with significant similarity. Southern analysis confirmed a syntenous relationship in the wheat and rice genomic regions and defined precisely the position of the breakpoint in ph2a. What seems to be a terminal deletion on 3DS is estimated to be approximately 80 Mb in length. We can tentatively predict the identification of approximately 220 genes from the region deleted in ph2a. The putative role of identified candidate Ph2 genes is discussed. The second approach explored the validity of recent proposals suggesting the presence of a meiotic gene cluster in the region of Ph2. The transcriptional characteristics of genes linked to Ph2 were investigated using data from wheat EST databases in combination with recently developed analysis software. The tissue - distribution of mRNAs derived from genes linked to Ph2 is shown to resemble that of other large chromosomal regions in the wheat genome. It is concluded that the apparently high number of genes from the Ph2 region expressed in wheat meiotic tissue is not indicative of a meiotic gene cluster in this region, but rather highlights the transcriptional complexity of meiotic anther tissue. Finally, the meiotic expression pattern of approximately 1800 wheat genes was examined using cDNA microarrays. Two approaches were… Advisors/Committee Members: Langridge, Peter (advisor), Houben, Andreas (advisor), School of Agriculture and Wine (school).

Subjects/Keywords: wheat; wheat genetics; meiosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sutton, T. J. (2003). Genomics approach to investigate the molecular control of meiosis in Triticum aestivum. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/37762

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sutton, Timothy J. “Genomics approach to investigate the molecular control of meiosis in Triticum aestivum.” 2003. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/37762.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sutton, Timothy J. “Genomics approach to investigate the molecular control of meiosis in Triticum aestivum.” 2003. Web. 15 Apr 2021.

Vancouver:

Sutton TJ. Genomics approach to investigate the molecular control of meiosis in Triticum aestivum. [Internet] [Thesis]. University of Adelaide; 2003. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/37762.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sutton TJ. Genomics approach to investigate the molecular control of meiosis in Triticum aestivum. [Thesis]. University of Adelaide; 2003. Available from: http://hdl.handle.net/2440/37762

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

19. Bian, Xue-Yu. Towards cloning the self-incompatibility genes from Phalaris coerulescens.

Degree: 2001, University of Adelaide

URL: http://hdl.handle.net/2440/37906

► Self-incompatibility (SI) is an important genetic mechanism to prevent the inbreeding of flowering plants and also an excellent system for studying cell-cell recognition and signal… (more)

▼ Self-incompatibility (SI) is an important genetic mechanism to prevent the inbreeding of flowering plants and also an excellent system for studying cell-cell recognition and signal transduction. During evolution, several SI systems have been evolved. A unique SI system widely spreads in the grasses. In the grasses, two unlinked, multi-allelic loci (S and Z) determine SI specificity. A putative self-incompatibility gene (Bm2) was previously cloned. In this study, the role of Bm2 in self-incompatibility was investigated first. The cDNA homologues of Bm2 were sequenced from two pollen-only mutants. The results indicated that Bm2 is not the one of SI genes in Phalaris, but represents a subclass of thioredoxin h. Thus a map-based cloning strategy was then adopted to clone the SI genes from Phalaris. Fine linkage maps of the S and Z regions were constructed. RFLP probes from wheat, barley, oat and rye were screened and the S locus was delimited to 0.26 cM and the Z locus to 1.0 cM from one side using specially designed segregating populations. The S locus was located to the sub-centromere region of triticeae chromosome group 1 and the Z locus to the middle of the long arm of group 2. Finally, barley and rice bacterial artificial chromosome (BAC) clones corresponding to the S and Z region were identified to analyse the chromosome structures and to seek candidate SI genes. The abundant repetitive sequences in the identified barley BAC clones limit their usefulness. Identification of Rice BAC clones orthologous to the S and Z regions open the gate to use rice genome information to clone SI genes from the grasses. A positive rice clone (139.9 kb) orthologous to the S region contained 19 predicted genes. Several of these genes might be involved in pollen tube germination and pollen-stigma interaction, which are the major parts of SI reaction. A positive clone (118.9 kb) orthologous to the Z region gave 16 predicted genes. The predicted genes on the outmost ends of these clones could be used to construct contigs to cover the S and Z regions and delimit the S and Z loci in the grasses. Advisors/Committee Members: Langridge, Peter (advisor), Barker, Susan Jane (advisor), Department of Plant Science (school).

Subjects/Keywords: Phalaris genetics; Phalaris pollen; Phalaris coerulescens; distorted segregating mapping; synteny mapping; map-based cloning

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bian, X. (2001). Towards cloning the self-incompatibility genes from Phalaris coerulescens. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/37906

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bian, Xue-Yu. “Towards cloning the self-incompatibility genes from Phalaris coerulescens.” 2001. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/37906.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bian, Xue-Yu. “Towards cloning the self-incompatibility genes from Phalaris coerulescens.” 2001. Web. 15 Apr 2021.

Vancouver:

Bian X. Towards cloning the self-incompatibility genes from Phalaris coerulescens. [Internet] [Thesis]. University of Adelaide; 2001. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/37906.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bian X. Towards cloning the self-incompatibility genes from Phalaris coerulescens. [Thesis]. University of Adelaide; 2001. Available from: http://hdl.handle.net/2440/37906

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

20. Jabbari, Jafar Sheikh. Molecular characterisation of differentially expressed genes in the interaction of barley and Rhynchosporium secalis.

Degree: 2009, University of Adelaide

URL: http://hdl.handle.net/2440/57718

► The barley scald pathogen (Rhynchosporium secalis) causes extensive economic losses, not only through lost product and quality, but also due to costs associated with chemical… (more)

▼ The barley scald pathogen (Rhynchosporium secalis) causes extensive economic losses, not only through lost product and quality, but also due to costs associated with chemical control. Economic and environmental impacts and the emerging resistance to fungicides and dominant resistance genes are reasons to understand molecular defence responses in order to develop new strategies to increase resistance of barley to this pathogen. In most pathosystems, defence gene expression in susceptible or resistant genotypes commonly differs quantitatively. Thus, differentially expressed genes between genotypes contrasting for response to infection by pathogens are considered candidate genes that have a role in resistance. This thesis presents functional analysis of a subset of genes isolated from a Suppression Subtractive Hybridisation library. The library was previously established and enriched for differentially expressed genes in epidermis of resistant and susceptible near-isogenic barley cultivars inoculated with R. secalis. Functional characterisation involved both investigating their putitative biochemical function as well as the genes‟ role(s) in biotic and abiotic stress responses. Three cDNA clones from the library were selected based on the putative function of the encoded proteins and the full length of the clones and their homologues were isolated from cDNA and genomic DNA. One of the clones represented a member of the pathogenesis-related protein family 17 (PR-17). Southern hybridisation showed that a small multigene family encodes the barley PR-17 proteins. Three members were cloned with two of them being novel. The second clone was homologous to galactinol synthases (GolS) and Southern blot analysis indicated existence of two GolS genes in the barley genome and subsequently two HvGolS members were isolated. The last clone (a single gene) showed similarity to very long chain fatty acid elongases, which indicates its involvement in synthesis of cuticular waxes. A characterised Arabidopsis mutant named fiddlehead (Atfdh) was highly similar to this gene and it was named HvFdh. Detailed expression analysis using Q-PCR, Northern blot analysis and publically available microarray data revealed that the isolated genes are regulated in response to a variety of abiotic and biotic stresses as well as different tissues during barley development. Under some treatments expression patterns were consistent with their putative roles and in agreement with results of other studies. Nevertheless, in other treatments expression profiles were not in agreement with previous findings in other plants indicating potentially different stress adaptation mechanisms between species. Further insight into the function of the encoded proteins was gained by their subcellular localisation using transient expression as GFP fusion proteins followed by confocal laser scanning microscopy. The results were in agreement with in silico predictions and their putative cellular function. In addition, a comprehensive list of homologous genes from other species… Advisors/Committee Members: Langridge, Peter (advisor), Oldach, Klaus (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: Scald; Barley; Rhynchosporium secalis; Disease resistance; Fatty acid elongase; Galactinol synthase; Pathogenesis related protein; Gene expression; Phytogenetic analysis; Subcellular localisation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jabbari, J. S. (2009). Molecular characterisation of differentially expressed genes in the interaction of barley and Rhynchosporium secalis. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/57718

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jabbari, Jafar Sheikh. “Molecular characterisation of differentially expressed genes in the interaction of barley and Rhynchosporium secalis.” 2009. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/57718.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jabbari, Jafar Sheikh. “Molecular characterisation of differentially expressed genes in the interaction of barley and Rhynchosporium secalis.” 2009. Web. 15 Apr 2021.

Vancouver:

Jabbari JS. Molecular characterisation of differentially expressed genes in the interaction of barley and Rhynchosporium secalis. [Internet] [Thesis]. University of Adelaide; 2009. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/57718.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jabbari JS. Molecular characterisation of differentially expressed genes in the interaction of barley and Rhynchosporium secalis. [Thesis]. University of Adelaide; 2009. Available from: http://hdl.handle.net/2440/57718

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

21. Kuchel, Haydn. The genetic analysis and manipulation of economically important traits in bread wheat.

Degree: 2008, University of Adelaide

URL: http://hdl.handle.net/2440/58575

► The aims of this thesis were to firstly gain an improved understanding of the genetic basis to economically important complex traits in bread wheat, and… (more)

▼ The aims of this thesis were to firstly gain an improved understanding of the genetic basis to economically important complex traits in bread wheat, and secondly, to investigate marker assisted selection (MAS) methodologies that may lead to improved rates of genetic gain. An elite Australian breeder's line, 'Stylet', and its parents 'Trident' and 'Molineux' were used as the basis of this study. A doubled-haploid (DH) population previously produced from a cross between 'Trident' and 'Molineux' (TIM DH) was used to dissect the genetic basis to end-use quality and agronomic performance. The study of end-use quality confirmed the widely published relationship between the glutenin loci and dough rheology. However this study also identified a quantitative trait locus (QTL) on chromosome 2A that was shown to be associated with dough resistance and baking quality, and another QTL on 3A that was associated with baking quality. QTL were identified in the T/M DH population that were involved in the control of time to ear-emergence through their effects on vernalisation sensitivity, photoperiod sensitivity and earliness per se. In addition to the well characterised Vrn-A1 and Ppd-B1 genes, six other QTL were identified. Three of these, QPpd.agt-1A, QPpd.agt-7A and QPpd.agt-7B are putative new loci involved in the control of photoperiod sensitivity in wheat. QPpd.agt-1A appears homoeologous to the photoperiod response gene Ppd-H2 in barley. QPpd.agt-7A and QPpd.agt-7B are located in homoeologous regions, and may represent a new phenology gene series in wheat. The T/M DH population was also used to dissect the genetic basis to grain yield and grain yield components, and to examine the influence of QTL-by environmental covariable interaction on genotype-by-environment interaction. The association of plant height genes, rust resistance genes and phenology genes with grain yield were determined. Overall, semi-dwarf rust resistant DH lines, carrying alleles conferring a short time to ear-emergence, showed the highest and most stable grain yield. Nine genetic associations with grain yield, without effects on plant height, time to ear-emergence and rust resistance, were identified. Two QTL, QGyld.agt-1B and QGyld.agt-4D were shown to have large and frequent associations with grain yield. QGyld.agt-1B showed only low levels of interaction with environmental covariables and therefore constitutes a prime candidate for MAS for grain yield. The second part of this study investigated the potential role of MAS through a practical breeding strategy and by computer simulation. An 'Anneullo/2*Stylet' cross aimed at producing a rust resistant 'Stylet' derivative with improved end-use quality was used as the model for this analysis. MAS was shown to be highly effective at improving the rate of genetic gain for rust resistance and end-use quality. This was most evident when undertaken on the BC₁F₁ population, although MAS also improved the efficiency of the breeding programme when performed on fixed lines. Practical implementation of the… Advisors/Committee Members: Langridge, Peter (advisor), School of Agriculture, Food and Wine : Plant and Pest Science (school).

Subjects/Keywords: Wheat Genetics.; Wheat Economic aspects.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kuchel, H. (2008). The genetic analysis and manipulation of economically important traits in bread wheat. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/58575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kuchel, Haydn. “The genetic analysis and manipulation of economically important traits in bread wheat.” 2008. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/58575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kuchel, Haydn. “The genetic analysis and manipulation of economically important traits in bread wheat.” 2008. Web. 15 Apr 2021.

Vancouver:

Kuchel H. The genetic analysis and manipulation of economically important traits in bread wheat. [Internet] [Thesis]. University of Adelaide; 2008. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/58575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kuchel H. The genetic analysis and manipulation of economically important traits in bread wheat. [Thesis]. University of Adelaide; 2008. Available from: http://hdl.handle.net/2440/58575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

22. Izanloo, Ali. Evaluation of physiological traits and identification of QTLs for drought tolerance in hexaploid wheat (Triticum aestivum L.).

Degree: 2008, University of Adelaide

URL: http://hdl.handle.net/2440/58242

► This study comprised three major parts: a comparative physiological study of drought responses under controlled conditions; a genetic study to construct the skeleton map of… (more)

▼ This study comprised three major parts: a comparative physiological study of drought responses under controlled conditions; a genetic study to construct the skeleton map of a doubled haploid (DH) population; and a quantitative trait loci (QTL) analysis to identify QTLs associated with drought tolerance traits in the field. In the first part (Chapter 3), three cultivars of wheat (Triticum aestivum L.) adapted to South Australian conditions were tested for drought tolerance under cyclic drought in growth rooms and glasshouse. Extensive physiological traits, including stomatal conductance, chlorophyll content and fluorescence, ABA content, water status traits (e.g. osmotic adjustment, RWC and leaf water potential), water soluble carbohydrates (WSC) and carbon isotope discrimination (Δ¹ ³C) were measured during experiments. Through these experiments, the drought responses of the three cultivars were physiologically dissected and the likely processes contributing most to drought tolerance were identified. In the South Australian wheatbelt, cyclic drought is a frequent event, represented by intermittent periods of rainfall which can occur around anthesis and post-anthesis in wheat. Three South Australian bread wheat cultivars, Excalibur, Kukri and RAC875, were evaluated in two growth room experiments under cyclic water-limiting conditions. In the first experiment, where plants were subjected to severe water stress, RAC875 and Excalibur (drought tolerant) showed significantly (P < 0.05) higher grain yield under cyclic water availability compared to Kukri (drought susceptible), producing 44% and 18% more grain yield compared to Kukri, respectively. In the second growth room experiment, where plants were subjected to a milder drought stress, the differences between cultivars were less pronounced, with only RAC875 showing significantly higher grain yield under the cyclic water treatment. Grain number per spike and the percentage of aborted tillers were the major yield components that affected yield under cyclic water stress. Excalibur and RAC875 adopted different morpho-physiological traits and mechanisms to reduce water stress. Excalibur was most responsive to cyclic water availability and showed the highest level of osmotic adjustment (OA), highest stomatal conductance, lowest ABA content and most rapid recovery from stress under cyclic water stress. RAC875 was more ‘conservative’ in its responses, with moderate OA, high leaf waxiness, high chlorophyll content and slower recovery from stress. Within this germplasm, the capacity for osmotic adjustment was the main physiological attribute associated with tolerance under cyclic water stress, which enabled plants to recover from water deficit. In the second part (Chapter 4), the genetic linkage map of a DH population including 368 lines, which was developed from a cross between ‘RAC875’ and ‘Kukri’, was constructed. The genetic linkage map consisted of about 500 molecular markers including ~300 DArT (Diversity array technology) and ~200 SSR (Microsattelite markers). … Advisors/Committee Members: Tester, Mark Alfred (advisor), Schnurbusch, Thorsten (advisor), Langridge, Peter (advisor), Condon, Anthony G. (advisor), School of Agriculture, Food and Wine : Plant and Pest Science (school).

Subjects/Keywords: drought; wheat; linkage map; quantitative trait loci (QTL); Wheat Genetics.; Wheat Growth.

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Izanloo, A. (2008). Evaluation of physiological traits and identification of QTLs for drought tolerance in hexaploid wheat (Triticum aestivum L.). (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/58242

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Izanloo, Ali. “Evaluation of physiological traits and identification of QTLs for drought tolerance in hexaploid wheat (Triticum aestivum L.).” 2008. Thesis, University of Adelaide. Accessed April 15, 2021. http://hdl.handle.net/2440/58242.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Izanloo, Ali. “Evaluation of physiological traits and identification of QTLs for drought tolerance in hexaploid wheat (Triticum aestivum L.).” 2008. Web. 15 Apr 2021.

Vancouver:

Izanloo A. Evaluation of physiological traits and identification of QTLs for drought tolerance in hexaploid wheat (Triticum aestivum L.). [Internet] [Thesis]. University of Adelaide; 2008. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2440/58242.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Izanloo A. Evaluation of physiological traits and identification of QTLs for drought tolerance in hexaploid wheat (Triticum aestivum L.). [Thesis]. University of Adelaide; 2008. Available from: http://hdl.handle.net/2440/58242

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations