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1.
Graaf, J.J. de.
Kisspeptin signaling and its role in canine reproduction: a basic understanding.
Degree: 2012, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/289517 
► There are several central and peripheral mechanisms that influence the GnRH secretion. For a long time it has been a mystery how these mechanisms influence…
(more)
▼ There are several central and peripheral mechanisms that influence the GnRH secretion. For a long time it has been a mystery how these mechanisms influence the GnRH neurons. In recent years there have been exciting new discoveries in this field, especially with the discovery of kisspeptin and its receptor. Kisspeptin is a neuropeptide that seems to have an important role in the regulation of GnRH neurons in the hypothalamus in various mammalian species. It is, among other things, supposed to be involved in the onset of puberty and in the mediation of the positive feedback exhibited by estrogens on the GnRH neurons in females, which leads to the GnRH/LH surge.
Although a lot of research has already been done, to our best knowledge, kisspeptin and its receptor have not yet been researched in the dog. Because the kisspeptin signaling has been called a major gatekeeper of the gonadotropic axis, it would be interesting to discover its role in the canine reproduction. To lessen the amount of dogs that are yearly euthanized all over the world because of overpopulation, it is important to find a non-surgical method of contraception that prevents estrous cyclicity. The kisspeptin signaling pathway could be a suitable target for developing an effective, safe, long-term or permanent non-surgical contraceptive for the bitch.
The aims of the present study were to identify the canine KISS1 and KISS1R genes and to characterize the amino acid sequences of canine kisspeptin and the kisspeptin receptor. The identification and characterization were done in silico. The results show that the KiSS1 and the KiSS1R gene are present at the canine genome and a large homology is found between the canine peptides and the peptides found in other mammalian species. This gives a basis to believe that kisspeptin signaling is also important in GnRH secretion in the dog and that it may indeed be a suitable target for a non-surgical contraceptive.
Advisors/Committee Members: Wolthers%2C%20C.
H.
J.%22%29&pagesize-30">
Albers-
Wolthers,
C.
H.
J..
Subjects/Keywords: Canine; dog; bitch; kisspeptin; reproduction
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APA (6th Edition):
Graaf, J. J. d. (2012). Kisspeptin signaling and its role in canine reproduction: a basic understanding. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/289517
Chicago Manual of Style (16th Edition):
Graaf, J J de. “Kisspeptin signaling and its role in canine reproduction: a basic understanding.” 2012. Masters Thesis, Universiteit Utrecht. Accessed January 22, 2021.
http://dspace.library.uu.nl:8080/handle/1874/289517.
MLA Handbook (7th Edition):
Graaf, J J de. “Kisspeptin signaling and its role in canine reproduction: a basic understanding.” 2012. Web. 22 Jan 2021.
Vancouver:
Graaf JJd. Kisspeptin signaling and its role in canine reproduction: a basic understanding. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Jan 22].
Available from: http://dspace.library.uu.nl:8080/handle/1874/289517.
Council of Science Editors:
Graaf JJd. Kisspeptin signaling and its role in canine reproduction: a basic understanding. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/289517
2.
Buitelaar, C.
Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch.
Degree: 2013, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/281034
► Background: Kisspeptins are recognized to be regulators within the hypothalamus-pituitary-gonadal axis and stimulators of GnRH and gonadotrophin secretion in mammals. In mice and sheep it…
(more)
▼ Background: Kisspeptins are recognized to be regulators within the hypothalamus-pituitary-gonadal axis and stimulators of GnRH and gonadotrophin secretion in mammals. In mice and sheep it is shown that mRNA expression of the Kiss1 gene and Kiss1R gene, as well as plasma LH concentrations varies between cycle phases. Little is known about sensitivity to kisspeptin between different cycle phases in the dog. Therefore the aim of the study is to find out whether there is a difference in sensitivity to kisspeptin throughout the different stages of the canine estrous cycle and anestrus.
Methods: Beagle bitches were treated intravenously with cKP10 (0,5 µg/kg) during anestrus, follicular phase and luteal phase (PIP and PDP). Plasma LH concentrations are measured.
Results: In each experimental phase, plasma LH concentrations increased significantly after cKP10 administration. LH response peaks were higher during anestrus and PDP compared to follicular phase and PIP.
Conclusion: LH responses show that sensitivity to kisspeptin differs during the estrous cycle and anestrus of the Beagle bitch. LH response is high during anestrus and PDP compared to follicular phase and PIP. More research should be done to confirm our findings and the meaning of high plasma LH response during PDP should be further investigated.
Advisors/Committee Members: Wolthers%2C%20C.
H.
J.%22%29&pagesize-30">
Albers-
Wolthers,
C.
H.
J..
Subjects/Keywords: Diergeneeskunde; Kisspeptin, dog, GnRH, luteal phase
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APA (6th Edition):
Buitelaar, C. (2013). Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/281034
Chicago Manual of Style (16th Edition):
Buitelaar, C. “Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch.” 2013. Masters Thesis, Universiteit Utrecht. Accessed January 22, 2021.
http://dspace.library.uu.nl:8080/handle/1874/281034.
MLA Handbook (7th Edition):
Buitelaar, C. “Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch.” 2013. Web. 22 Jan 2021.
Vancouver:
Buitelaar C. Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch. [Internet] [Masters thesis]. Universiteit Utrecht; 2013. [cited 2021 Jan 22].
Available from: http://dspace.library.uu.nl:8080/handle/1874/281034.
Council of Science Editors:
Buitelaar C. Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch. [Masters Thesis]. Universiteit Utrecht; 2013. Available from: http://dspace.library.uu.nl:8080/handle/1874/281034
Universiteit Utrecht
3.
Walen, M.
The efficacy of kisspeptin antagonists p234 and p271 in blocking the response to KP-10 in vivo in the bitch and in vitro using flow-cytometry.
Degree: 2014, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/294313
► BACKGROUND: The neuropeptide kisspeptin and its receptor GPR54 play a crucial role in the reproductive function of mammals. Finding an efficient kisspeptin antagonist could be…
(more)
▼ BACKGROUND: The neuropeptide kisspeptin and its receptor GPR54 play a crucial role in the reproductive function of mammals. Finding an efficient kisspeptin antagonist could be the first step towards a new method for non-surgical contraception in the dog. The aims of this study were to test kisspeptin antagonists 271 and 234 in vitro using flow-cytometry and to test p271 in vivo during different stages of the estrous cycle of the bitch. METHODS: In vitro: Chem-1 cells expressing the human GPR54 receptor were labeled with Fluo-3 AM, a fluorescence indicator for intracellular calcium, and examined by flow-cytometry. A concentration response study of hKP-10 and cKP-10 was designed to test if flow-cytometry is a reliable method for measuring intracellular calcium (as a measure for receptor activation) and to determine the optimal hKP-10 concentration. This data were then used to test the antagonists p234 and p271 in vitro. In vivo: P271 was tested during different stages of the estrous cycle of the bitch. In this study only the results in the follicular phase are discussed. Female dogs received 50 µg/kg/hour p271 by iv infusion for three hour. Two hours after the start of the infusion 0.5 µg/kg cKP-10 was iv administered. Controls received one iv injection of 0.5 µg/kg cKP-10. Blood samples before, during and after the peptide administration were collected for the determination of plasma [LH]. RESULTS: Both human and canine KP-10 gave a typical sigmoidal concentration-response curve in vitro. We found no antagonistic or agonist effect of peptide 234 and 271 in vitro. Furthermore, p271 was not able to reduce basal plasma LH concentrations and the LH response to cKP-10 in vivo in the bitch during follicular phase. CONCLUSION: P271 is probably not a good kisspeptin antagonist for the dog. Flow-cytometric calcium flux assays seem like a reliable method to study kisspeptin receptor activation in vitro.
Advisors/Committee Members: Wolthers%2C%20C.
H.
J.%22%29&pagesize-30">
Albers-
Wolthers,
C.
H.
J..
Subjects/Keywords: Kisspeptin; KP-10; GPR54; antagonist; p234; p271; in vitro; flow-cytometry; in vivo; bitch
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APA (6th Edition):
Walen, M. (2014). The efficacy of kisspeptin antagonists p234 and p271 in blocking the response to KP-10 in vivo in the bitch and in vitro using flow-cytometry. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/294313
Chicago Manual of Style (16th Edition):
Walen, M. “The efficacy of kisspeptin antagonists p234 and p271 in blocking the response to KP-10 in vivo in the bitch and in vitro using flow-cytometry.” 2014. Masters Thesis, Universiteit Utrecht. Accessed January 22, 2021.
http://dspace.library.uu.nl:8080/handle/1874/294313.
MLA Handbook (7th Edition):
Walen, M. “The efficacy of kisspeptin antagonists p234 and p271 in blocking the response to KP-10 in vivo in the bitch and in vitro using flow-cytometry.” 2014. Web. 22 Jan 2021.
Vancouver:
Walen M. The efficacy of kisspeptin antagonists p234 and p271 in blocking the response to KP-10 in vivo in the bitch and in vitro using flow-cytometry. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Jan 22].
Available from: http://dspace.library.uu.nl:8080/handle/1874/294313.
Council of Science Editors:
Walen M. The efficacy of kisspeptin antagonists p234 and p271 in blocking the response to KP-10 in vivo in the bitch and in vitro using flow-cytometry. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/294313
Universiteit Utrecht
4.
Elderen, M. van.
Dose-response study with canine kisspeptin-10 in vivo and in vitro.
Degree: 2013, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/289498
► Kisspeptin stimulates gonadotropic-releasing hormone (GnRH) and is a key player in puberty onset and maintenance of normal reproductive function in several mammalian species. However, doses…
(more)
▼ Kisspeptin stimulates gonadotropic-releasing hormone (GnRH) and is a key player in puberty onset and maintenance of normal reproductive function in several mammalian species. However, doses below 1 μg/kg BW have not been studied in dogs. The aims of this study were to find the lowest dose of canine kisspeptin-10 that significantly increases the plasma LH concentration above the 95 percentile of the measured basal plasma LH concentrations in dogs and to investigate the corresponding effects of human kisspeptin-10 (hKP10) and canine kisspeptin-10 (cKP10) in vitro. cKP10 was administered intravenously at weekly intervals to adult Beagle bitches during anestrus in doses of 0.1, 0.5, 1 and 10 μg/kg BW. Blood samples were collected at 40 and 0 minutes before and at 10, 20, 30, 40, 60, 90, and 120 minutes after KP10 administration for measurement of plasma LH concentration. The doses 0.5, 1 and 10 μg/kg BW resulted in an increase in plasma LH concentration above the 95 percentile of the measured basal plasma LH concentrations. Chem-1 cells transfected with GPR54 cDNA were exposed to 10-6,10-8 or 10-10 M hKP10 or cKP10 and the maximum amplitude of intracellular Ca2+ concentrations, [Ca2+]i, was calculated for each cell. The maximum amplitudes of [Ca2+]i differed between hKP10 and cKP10 for only 10-8 M. In conclusion, 0.5 μg/kg BW cKP10 is the lowest dose that significantly increases the plasma LH concentration above the 95 percentile of the measured basal plasma LH concentrations in the dog and hKP10 and cKP10 give a similar [Ca2+]i response after binding to the human kisspeptin receptor GPR54 in vitro.
Advisors/Committee Members: Wolthers%2C%20C.
H.
J.%22%29&pagesize-30">
Albers-
Wolthers,
C.
H.
J..
Subjects/Keywords: KP10; LH; canine; dog
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APA (6th Edition):
Elderen, M. v. (2013). Dose-response study with canine kisspeptin-10 in vivo and in vitro. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/289498
Chicago Manual of Style (16th Edition):
Elderen, M van. “Dose-response study with canine kisspeptin-10 in vivo and in vitro.” 2013. Masters Thesis, Universiteit Utrecht. Accessed January 22, 2021.
http://dspace.library.uu.nl:8080/handle/1874/289498.
MLA Handbook (7th Edition):
Elderen, M van. “Dose-response study with canine kisspeptin-10 in vivo and in vitro.” 2013. Web. 22 Jan 2021.
Vancouver:
Elderen Mv. Dose-response study with canine kisspeptin-10 in vivo and in vitro. [Internet] [Masters thesis]. Universiteit Utrecht; 2013. [cited 2021 Jan 22].
Available from: http://dspace.library.uu.nl:8080/handle/1874/289498.
Council of Science Editors:
Elderen Mv. Dose-response study with canine kisspeptin-10 in vivo and in vitro. [Masters Thesis]. Universiteit Utrecht; 2013. Available from: http://dspace.library.uu.nl:8080/handle/1874/289498
Universiteit Utrecht
5.
Daniël, J.W.
Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271.
Degree: 2014, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/289058
► Kisspeptin is an important regulator of the hypothalamic-pituitary-gonadal axis in mammals. It acts on the GnRH neurons trough the GPR54 receptor initiating GnRH release. The…
(more)
▼ Kisspeptin is an important regulator of the hypothalamic-pituitary-gonadal axis in mammals. It acts on the GnRH neurons trough the GPR54 receptor initiating GnRH release. The aim of this study is to determine the optimal concentration of kisspeptin-10 to activate the GPR54 receptor in vitro. This data is then used for in vitro testing of the kisspeptin antagonist P271. The GPR54 receptor is a G-protein coupled receptor, which when activated raises the intracellular calcium level. We used a single-cell fluorescence microscopy on Chem-1 cells (expressing the human GPR54 receptor) loaded with the calcium-sensitive fluorescent dye Fura-2. We were unable to create a reproducible concentration-response study, however we found two concentrations of kisspeptin-10 that gave a reproducible peak (10-6M and 10-7M). These concentrations were used to test the antagonist P271. In this study peptide P271 showed agonistic properties and no antagonistic properties. This could be because of the method used in these experiments.
Advisors/Committee Members: Wolthers%2C%20C.
H.
J.%22%29&pagesize-30">
Albers-
Wolthers,
C.
H.
J..
Subjects/Keywords: Kisspeptin; GPR54; Antagonist; P271; in vitro; single-cell fluorescence microscopy; fura-2-AM
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APA (6th Edition):
Daniël, J. W. (2014). Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/289058
Chicago Manual of Style (16th Edition):
Daniël, J W. “Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271.” 2014. Masters Thesis, Universiteit Utrecht. Accessed January 22, 2021.
http://dspace.library.uu.nl:8080/handle/1874/289058.
MLA Handbook (7th Edition):
Daniël, J W. “Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271.” 2014. Web. 22 Jan 2021.
Vancouver:
Daniël JW. Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Jan 22].
Available from: http://dspace.library.uu.nl:8080/handle/1874/289058.
Council of Science Editors:
Daniël JW. Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/289058
6.
Brederode, J.C. van.
Progestins as a treatment for subinvolution of placental sites in the bitch.
Degree: 2012, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/289378
► Eight postpartum bitches diagnosed with subinvolution of placental sites (SIPS) were followed in this retrospective study to investigate the efficacy of treatment with low oral…
(more)
▼ Eight postpartum bitches diagnosed with subinvolution of placental sites (SIPS) were followed in this retrospective study to investigate the efficacy of treatment with low oral doses of progestins in stopping bloody vulvar discharge. Two other bitches diagnosed with SIPS were included in this study not receiving treatment.
A progestin derivate was administered orally once a day in low dose for two weeks, with half the daily dose reduced the second week. Cessation of vulvar discharge was seen in all eight treated dogs within this two week treatment period. The untreated dogs persisted with the problem respectively until a few weeks before and till onset of the next proestrus. No adverse effects on future heat, fertility, gestation and parturition was found in both the treated and untreated dogs. The results of this study indicate that oral low dose progestin therapy is effective and probably safe in treatment of SIPS.
Advisors/Committee Members: C.%22%29&pagesize-30">Schaefers-Okkens, A.
C.,
Wolthers%2C%20C.H.J.%22%29&pagesize-30">Albers-Wolthers, C.H.J..
Subjects/Keywords: SIPS; megestrolacetate; medroxyprogestroneacetate; trophoblast cells; progestins
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APA ·
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APA (6th Edition):
Brederode, J. C. v. (2012). Progestins as a treatment for subinvolution of placental sites in the bitch. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/289378
Chicago Manual of Style (16th Edition):
Brederode, J C van. “Progestins as a treatment for subinvolution of placental sites in the bitch.” 2012. Masters Thesis, Universiteit Utrecht. Accessed January 22, 2021.
http://dspace.library.uu.nl:8080/handle/1874/289378.
MLA Handbook (7th Edition):
Brederode, J C van. “Progestins as a treatment for subinvolution of placental sites in the bitch.” 2012. Web. 22 Jan 2021.
Vancouver:
Brederode JCv. Progestins as a treatment for subinvolution of placental sites in the bitch. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Jan 22].
Available from: http://dspace.library.uu.nl:8080/handle/1874/289378.
Council of Science Editors:
Brederode JCv. Progestins as a treatment for subinvolution of placental sites in the bitch. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/289378
. 
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