+publisher:"Università degli Studi di Milano" +contributor:("coordinatore: F. Gandolfi")

1. I. Alloggio. ¿STUDY OF THE BEHAVIOR OF LACTOCOCCUS LACTIS AND LISTERIA MONOCYTOGENES IN COMPETITION FOR FOOD SAFETY IN DAIRY PRODUCTS¿.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/487493

► Microbial competition is a mechanism that occurs when two or more microbial species compete for ecological niches to support their survival and growth. Different factors… (more)

▼ Microbial competition is a mechanism that occurs when two or more microbial species compete for ecological niches to support their survival and growth. Different factors can contribute to the outcome of microbial competition, such as molecules exchanged between the competing organisms for the regulation of cell densities and the initial spatial configuration of the microbe–microbe interaction. Specifically, production of compounds that kill or limit the growth of competing strains or species can promote niche monopolization [2]. The released compounds include secondary metabolite antibiotics, bacterial peptides or low-molecular-mass organic compounds. The same happens in food, and it could be possible to explore this 'molecular' communication to improve food safety. In that sense, it is very important to develop tools in the control of bacterial species for a better food safety. The present work focused on the study of microbial competition between Listeria monocytogenes (LM) and Lactococcus lactis (LAC) monitored through proteomics, peptidomics and metabolomics approach. We study the secretome of these two microorganisms (Listeria monocytogenes and Lactococcus lactis) alone and in co-culture. In particular, we studied by proteomic analysis the evaluation of proteins secreted by bacteria through one/two-dimensional electrophoresis coupled to mass spectrometry (MALDI TOF). Furthermore, in order to characterize each secretome, label free Shotgun analysis was conducted using nano UPLC-MS system. Furthermore, the secretome of these microorganisms has been studied through first an untargeted proteomics analysis in vitro, followed by validation directly in a system resuming cheese. The objective of the last part of the project has been the monitoring of bacterial competition between through a combination of microbial Imaging mass spectrometry and LC-MS/MS, in order to investigate the metabolic profile of each bacteria in the interacting microbial colonies. In according with obtained preliminary data (one-dimensional and two-dimensional electrophoresis), new data highlighted, during competition, the higher production by Listeria monocytogenes of moonlighting protein Enolase (C1KY94) and Glucose 6 Phosphate isomerase (Q71X61), of Septation ring formation regulator EzrA (B8DHE7), involved into cell replication in regulatory mechanisms of cell energetics or metabolism and the lower secretion Endopeptidase P60 (P21171), protein associated with the cell surface and involved in the process of invasion. In parallel, L. lactis produced higher amounts of Page 6 of 114 Secreted 45 kDa protein and switched from lantibiotic Nisin A production to Nisin Z production. In competition with LM, LAC strain investigated produce higher amounts of Secreted 45 kDa protein with peptidoglycan lytic activity and the selective secretion of Nisin Z probably to improve lantibiotic solubility in less acidic environment. Lastly, IMS analysis revealed several interesting compounds during interaction of microbial colonies. At least six compounds are… Advisors/Committee Members: tutor: L. Bonizzi, coordinatore: F. Gandolfi, BONIZZI, LUIGI.

Subjects/Keywords: Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA (6^th Edition):

Alloggio, I. (2017). ¿STUDY OF THE BEHAVIOR OF LACTOCOCCUS LACTIS AND LISTERIA MONOCYTOGENES IN COMPETITION FOR FOOD SAFETY IN DAIRY PRODUCTS¿. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/487493

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alloggio, I.. “¿STUDY OF THE BEHAVIOR OF LACTOCOCCUS LACTIS AND LISTERIA MONOCYTOGENES IN COMPETITION FOR FOOD SAFETY IN DAIRY PRODUCTS¿.” 2017. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/487493.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alloggio, I.. “¿STUDY OF THE BEHAVIOR OF LACTOCOCCUS LACTIS AND LISTERIA MONOCYTOGENES IN COMPETITION FOR FOOD SAFETY IN DAIRY PRODUCTS¿.” 2017. Web. 24 Jan 2021.

Vancouver:

Alloggio I. ¿STUDY OF THE BEHAVIOR OF LACTOCOCCUS LACTIS AND LISTERIA MONOCYTOGENES IN COMPETITION FOR FOOD SAFETY IN DAIRY PRODUCTS¿. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/487493.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alloggio I. ¿STUDY OF THE BEHAVIOR OF LACTOCOCCUS LACTIS AND LISTERIA MONOCYTOGENES IN COMPETITION FOR FOOD SAFETY IN DAIRY PRODUCTS¿. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/487493

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. F. Dell'Orco. OF MILK AND HONEY: MOLECULAR TOOLS TO MONITOR RESISTANCE IN HONEYBEE AND DAIRY COW DISEASES.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/548755

► L’utilizzo di strumenti molecolari per il monitoraggio del rischio delle malattie e per le strategie di controllo degli agenti patogeni in campo è ancora sottovalutato… (more)

Subjects/Keywords: Apis mellifera; hygienic behavior; Nosema ceranae; Nosema apis; Escherichia coli; virulence factor; Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali

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APA (6^th Edition):

Dell'Orco, F. (2018). OF MILK AND HONEY: MOLECULAR TOOLS TO MONITOR RESISTANCE IN HONEYBEE AND DAIRY COW DISEASES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/548755

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dell'Orco, F.. “OF MILK AND HONEY: MOLECULAR TOOLS TO MONITOR RESISTANCE IN HONEYBEE AND DAIRY COW DISEASES.” 2018. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/548755.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dell'Orco, F.. “OF MILK AND HONEY: MOLECULAR TOOLS TO MONITOR RESISTANCE IN HONEYBEE AND DAIRY COW DISEASES.” 2018. Web. 24 Jan 2021.

Vancouver:

Dell'Orco F. OF MILK AND HONEY: MOLECULAR TOOLS TO MONITOR RESISTANCE IN HONEYBEE AND DAIRY COW DISEASES. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/548755.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dell'Orco F. OF MILK AND HONEY: MOLECULAR TOOLS TO MONITOR RESISTANCE IN HONEYBEE AND DAIRY COW DISEASES. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/548755

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. G. Magro. BOVINE STAPHYLOCOCCUS AUREUS MASTITIS: FROM THE MAMMARY IMMUNE RESPONSE TO THE BACTERIA VIRULENCE GENES.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/548380

► Staphylococcus aureus (S. aureus) is one of the most important bacteria in veterinary medicine. In dairy herds, it is a contagious bacterium responsible mainly for… (more)

▼ Staphylococcus aureus (S. aureus) is one of the most important bacteria in veterinary medicine. In dairy herds, it is a contagious bacterium responsible mainly for subclinical mastitis in cattle, which frequently gives rise to persistent and chronic infection. Mastitis cause considerable economic losses due to i) decreased milk production, (ii) reduced milk quality, and (iii) treatment costs. Mastitis is also a public health problem. Indeed, the strains isolated from infected glands could produce enterotoxins. Three factors interact in mastitis: the host, the pathogen and the environment. This thesis focuses on two main aspects: the host immune response and the virulence factor of S. aureus. The first chapter of the thesis focused on the development of a new mammary gland model to study the innate immune response bacterial infection. The mammary gland is a complex organ, and the immune response is a consequence of the different cell population interactions. Continuous or primary epithelial cell lines have been extensively used to study the mammary gland immune response, but they are composed of a single cell population. Previous studies explored the tissues of lactating cows, unconsidering the possibility of an already triggered immune response. To investigate the innate immune response of the bovine mammary gland, we used an explant of healthy heifer gland. This model allowed us to: i) exclude previous exposure of the udder to microorganisms, which might have damaged the cells and/or triggered an immune response, and ii) consider the interaction of the challenging microorganism with the tissue cell populations. Our aim was to test whether this innovative model might be a valid model to investigate the innate immune response to infection. The study was carried out on 2 mm3-sections of heifer udders, in 2 consecutive trials, using LPS or LTA in the first trial and two different concentrations of S. aureus in the second. Treated and untreated sections were collected after 1h, 3h and 6h incubation; in the first trial, a final time-point at 18h was considered. The mRNA expression of TNFα, IL-1β, IL-6, IL-8 and LAP was analyzed by quantitative real-time PCR. Histological examination showed well-preserved morphology of the tissue, and apoptosis only showed a slight, not significant increase throughout the experiment. IL-1β and IL-6 were significantly up-regulated, in response to LPS or S. aureus, while TNF-α and IL-8 significantly increased only under LPS treatment. LAP expression showed a significant late increase when stimulated by LPS. The immunochemical staining of the sections demonstrated a higher number of T lymphocytes within the alveolar epithelium, in comparison with interstitial localization. Since the explants belonged to pubertal non-pregnant heifers, T cells may be regarded as resident cells, suggesting their participation in the regulation of mammary homeostasis. Therefore, applying our model would give new insights in the investigation of udder pathophysiology. The second chapter of the thesis focused… Advisors/Committee Members: tutor: R. PICCININI, coordinatore: F. GANDOLFI.

Subjects/Keywords: Bovine Mastitis; Staphylococcus aureus; virulence genes; innate immunity; ex vivo model; Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Magro, G. (2018). BOVINE STAPHYLOCOCCUS AUREUS MASTITIS: FROM THE MAMMARY IMMUNE RESPONSE TO THE BACTERIA VIRULENCE GENES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/548380

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Magro, G.. “BOVINE STAPHYLOCOCCUS AUREUS MASTITIS: FROM THE MAMMARY IMMUNE RESPONSE TO THE BACTERIA VIRULENCE GENES.” 2018. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/548380.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Magro, G.. “BOVINE STAPHYLOCOCCUS AUREUS MASTITIS: FROM THE MAMMARY IMMUNE RESPONSE TO THE BACTERIA VIRULENCE GENES.” 2018. Web. 24 Jan 2021.

Vancouver:

Magro G. BOVINE STAPHYLOCOCCUS AUREUS MASTITIS: FROM THE MAMMARY IMMUNE RESPONSE TO THE BACTERIA VIRULENCE GENES. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/548380.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Magro G. BOVINE STAPHYLOCOCCUS AUREUS MASTITIS: FROM THE MAMMARY IMMUNE RESPONSE TO THE BACTERIA VIRULENCE GENES. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/548380

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. A. Vanelli. ISOLATION, CHARACTERIZATION AND DIFFERENTIATION POTENTIAL OF CARDIAC PROGENITOR CELLS IN ADULT PIGS.

Degree: 2011, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/169553

► Cardiac progenitor cells have been recently identified in the post-natal heart. However, the experiments carried out so far were predominantly performed on mice and humans.… (more)

▼ Cardiac progenitor cells have been recently identified in the post-natal heart. However, the experiments carried out so far were predominantly performed on mice and humans. Considering the potential future application of these cells for human cell therapy, we propose the pig as an intermediate preclinical model, due to the morphological and functional affinity of the porcine species with the human. Pig adult cardiac progenitors were isolated from explants of adult pig hearts and cultured in complete DMEM medium. Cells were analyzed for their expansion ability and for the expression of surface markers. All lines were positive for CD44 and CD34. Only a small percentage of cells expressed CD31, while screening for CD45 gave a negative result in all the lines obtained, regardless to their origin. Molecular characterization was performed using primers designed for pluripotency, mesenchymal and cardiac related genes. These cells were shown to actively transcribe for c-kit, Oct4, Gata6, CD31, CD34, CD44, Mesp1, Mesp2, Mef2a, Nkx2.5, ANP, Cx43, Cardiac Actinin, Tbx5 and Tbx18. Staining with Desmin, Connexin43, MF20, Rhodamine Phalloidin, α-Tropomyosin and Smooth Muscle Actin antibodies demonstrated that adult pig cardiac progenitors could differentiate into cardiomyocytes and smooth muscle cells, when exposed to the appropriate inducing media. Fusion experiments revealed that pig cardiac progenitor cells have the ability to fuse with fetal rat cardiomyocytes, reflecting a distinct property common to many types of cardiac progenitors. The data obtained indicate that adult porcine cardiac progenitor cells can be isolated and cultured in vitro. These cells display a high molecular affinity and share many differentiation properties with a cardiac progenitor cell population recently described in weanling pigs. This suggests that cardiac progenitors, although more abundant and functional in the neonatal and post weaning period, are present and active in adult porcine hearts and may represent an interesting tool for preclinical and translational studies of stem cell-based cardiovascular therapy. Advisors/Committee Members: tutor: T.A.L. Brevini, coordinatore: F. Gandolfi, BREVINI, TIZIANA, GANDOLFI, FULVIO.

Subjects/Keywords: cardiovascular disease; adult cardiac progenitors; large animal model; pig; differentiation ability; cardiomyocytes; Settore BIO/13 - Biologia Applicata; Settore BIO/11 - Biologia Molecolare; Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vanelli, A. (2011). ISOLATION, CHARACTERIZATION AND DIFFERENTIATION POTENTIAL OF CARDIAC PROGENITOR CELLS IN ADULT PIGS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/169553

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vanelli, A.. “ISOLATION, CHARACTERIZATION AND DIFFERENTIATION POTENTIAL OF CARDIAC PROGENITOR CELLS IN ADULT PIGS.” 2011. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/169553.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vanelli, A.. “ISOLATION, CHARACTERIZATION AND DIFFERENTIATION POTENTIAL OF CARDIAC PROGENITOR CELLS IN ADULT PIGS.” 2011. Web. 24 Jan 2021.

Vancouver:

Vanelli A. ISOLATION, CHARACTERIZATION AND DIFFERENTIATION POTENTIAL OF CARDIAC PROGENITOR CELLS IN ADULT PIGS. [Internet] [Thesis]. Università degli Studi di Milano; 2011. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/169553.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vanelli A. ISOLATION, CHARACTERIZATION AND DIFFERENTIATION POTENTIAL OF CARDIAC PROGENITOR CELLS IN ADULT PIGS. [Thesis]. Università degli Studi di Milano; 2011. Available from: http://hdl.handle.net/2434/169553

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. M.M. Ferretti. DETERMINAZIONE DELLE CARATTERISTICHE FENOTIPICHE E GENOTIPICHE DI CEPPI DI STAPHYLOCOCCUS IN RELAZIONE A METICILLINO- E VANCOMICINO-RESISTENZA.

Degree: 2012, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/169559

► OBJECTIVE – The aim of the study was to evaluate the phenotypic and genotypic characteristics of methicillin-resistant Staphylococcus pseudintermedius in samples from dogs and cats… (more)

Subjects/Keywords: Staphylococcus pseudintermedius; methicillin-resistance; vancomycin-resistance; mecA; Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferretti, M. (2012). DETERMINAZIONE DELLE CARATTERISTICHE FENOTIPICHE E GENOTIPICHE DI CEPPI DI STAPHYLOCOCCUS IN RELAZIONE A METICILLINO- E VANCOMICINO-RESISTENZA. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/169559

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ferretti, M.M.. “DETERMINAZIONE DELLE CARATTERISTICHE FENOTIPICHE E GENOTIPICHE DI CEPPI DI STAPHYLOCOCCUS IN RELAZIONE A METICILLINO- E VANCOMICINO-RESISTENZA.” 2012. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/169559.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ferretti, M.M.. “DETERMINAZIONE DELLE CARATTERISTICHE FENOTIPICHE E GENOTIPICHE DI CEPPI DI STAPHYLOCOCCUS IN RELAZIONE A METICILLINO- E VANCOMICINO-RESISTENZA.” 2012. Web. 24 Jan 2021.

Vancouver:

Ferretti M. DETERMINAZIONE DELLE CARATTERISTICHE FENOTIPICHE E GENOTIPICHE DI CEPPI DI STAPHYLOCOCCUS IN RELAZIONE A METICILLINO- E VANCOMICINO-RESISTENZA. [Internet] [Thesis]. Università degli Studi di Milano; 2012. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/169559.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ferretti M. DETERMINAZIONE DELLE CARATTERISTICHE FENOTIPICHE E GENOTIPICHE DI CEPPI DI STAPHYLOCOCCUS IN RELAZIONE A METICILLINO- E VANCOMICINO-RESISTENZA. [Thesis]. Università degli Studi di Milano; 2012. Available from: http://hdl.handle.net/2434/169559

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. F. Albonico. ANALYSIS OF MICRORNAS AND GENIC POLYMORPHISMS IN THE DIAGNOSIS OF NEOPLASTIC AND TRANSMISSIBLE DISEASES IN DOGS.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/216646

► During my PhD, I mainly worked on two different fields of research: 1. Analysis of microRNAs in canine hematopoietic malignancies for diagnostic purposes. MicroRNAs (miRNAs)… (more)

▼ During my PhD, I mainly worked on two different fields of research: 1. Analysis of microRNAs in canine hematopoietic malignancies for diagnostic purposes. MicroRNAs (miRNAs) are a new class of small non-coding RNAs involved in the negative regulation of gene expression. In humans, it has been shown that miRNAs play a key role in the modulation of the innate immune response and in the regulation of normal and neoplastic hematopoiesis. The expression profile of these molecules varies in the different stages of differentiation of the hematopoietic system cellular components and any alteration of these processes is directly associated with dysregulation of one or more miRNAs. The frequency and nature of some of these tumors are similar in dogs and humans. In dog, the studies of hemato-oncology performed to date have shown that the currently used methodologies are not always able to obtain comprehensive information about the staging, grading, immunophenotyping and prognosis of the diseases. This makes miRNAs very attractive as new potential diagnostic and prognostic markers allowing better subclassification of these tumors in dogs. The purpose of the study undertaken in this project was to examine, using stem-loop TaqMan real time RT-PCR, the expression profile of a panel of miRNAs in some hematological malignancies of the dog, such as chronic lymphocytic leukemia (CLL), lymph node lymphoma and splenic lymphoma, in relation to their non cancer tissues. Panels of miRNAs, already known in humans because of their importance in hematopoietic malignancies, have been selected for investigation, according to the information available in the literature and databases, and to their level of conservation in the canine genome. As a preliminary step, for each studied canine neoplasia, an accurate validation of suitable candidate endogenous control (EC) genes for normalization of miRNA expression levels in normal and neoplastic lymphoid tissues was made. This was performed by means of real time RT-PCR followed by statistical analysis using the algorithms NormFinder and geNorm. In all studied lymphoid malignancies, and for each miRNA investigated, a relative quantification (by calculating the delta-delta Ct to the endogenous control gene selected) and an absolute quantification (interpolating its Ct value on standard curves obtained from serial dilutions of an equimolar pool of synthetic miRNAs) was performed after selecting the best housekeeping genes. The results of this study confirm that in dogs, as already shown in humans, there is differential expression of some miRNAs in various hematologic malignancies, suggesting the potential utility of miRNA monitoring as a new diagnostic strategy in canine hematopoietic malignancies. In particular, in lymph node lymphoma samples miR-181a was upregulated in canine T-cell lymphoma and miR-17-5p was upregulated in canine B-cell lymphoma. Moreover, the molar ratio between miR-181a and miR-17-5p clearly distinguished between T-cell and B-cell lymphoma samples. In CLL samples, the molar ratio… Advisors/Committee Members: tutor: M. Mortarino, coordinatore: F. Gandolfi, MORTARINO, MICHELE, GANDOLFI, FULVIO.

Subjects/Keywords: microRNA; canine; lymph nodal lymphoma; splenic lymphoma; CLL; HRMA; Staphylococcus pseudintermedius; Dirofilaria immitis; Dirofilaria repens; genic polymorphisms; Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Albonico, F. (2013). ANALYSIS OF MICRORNAS AND GENIC POLYMORPHISMS IN THE DIAGNOSIS OF NEOPLASTIC AND TRANSMISSIBLE DISEASES IN DOGS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/216646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Albonico, F.. “ANALYSIS OF MICRORNAS AND GENIC POLYMORPHISMS IN THE DIAGNOSIS OF NEOPLASTIC AND TRANSMISSIBLE DISEASES IN DOGS.” 2013. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/216646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Albonico, F.. “ANALYSIS OF MICRORNAS AND GENIC POLYMORPHISMS IN THE DIAGNOSIS OF NEOPLASTIC AND TRANSMISSIBLE DISEASES IN DOGS.” 2013. Web. 24 Jan 2021.

Vancouver:

Albonico F. ANALYSIS OF MICRORNAS AND GENIC POLYMORPHISMS IN THE DIAGNOSIS OF NEOPLASTIC AND TRANSMISSIBLE DISEASES IN DOGS. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/216646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Albonico F. ANALYSIS OF MICRORNAS AND GENIC POLYMORPHISMS IN THE DIAGNOSIS OF NEOPLASTIC AND TRANSMISSIBLE DISEASES IN DOGS. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/216646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. F. Saccone. CELL-BASED BIOASSAYS FOR TESTING BIOACTIVE COMPOUNDS IN FARM ANIMALS.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/216692

► Cell-based assays can be adopted as in vitro method to evaluate the bioavailability and functionality of different nutraceutical and bioactive compounds, particularly in view of… (more)

▼ Cell-based assays can be adopted as in vitro method to evaluate the bioavailability and functionality of different nutraceutical and bioactive compounds, particularly in view of the need to use alternatives to animal studies. The interest in these bioactive compounds in animal sciences is not only related to medical research. It also represents an enormous benefit for health food companies and the animal produce sector in general. The general aim of my PhD study was to study nutraceutical effects at a cellular level in response to different stress challenges. In the first section of my thesis, the protective role of α-tocopherol in counteracting the cytotoxicity and DNA damage induced by Ochratoxin A (OTA) in primary porcine fibroblast cell cultures (ear and embryo), was determined by using the MTT assay, LDH release, DNA fragmentation, and TUNEL stain. The aim of the second section was to evaluate the protective role of bovine Lactoferrin (bLf), added to the culture medium, against lipopolysaccharide (LPS) cytotoxicity using the established bovine mammary epithelial cell line BME-UV1 as an in vitro model of the bovine mammary epithelium. In addition, we assessed whether BME-UV1 cells were able to express endogenous bLf after in vitro exposure to LPS. A further objective of my thesis work was to use cell-based bioassays to investigate the plasmin-plasminogen system. This system plays a key role in cellular responses, and is involved both in physiological and in pathological conditions in the mammary gland. The aim of the third section was to determine the effect of growth factors (IGF-1 and EGF) and three hormones (insulin, dexamethasone, and prolactin) on the expression of plasminogen activator (PA)-related genes (u-PA, u-PAR, PAI-1, PAI-2) and BME-UV1 cell proliferation. In addition we investigated the effects of E. coli LPS on cell viability, the modulation of cell-associated u-PA activity and the regulation of u-PA and u-PAR RNA expression in BME-UV1 cells. Below are more details on what each section covers: The first section reports how the role of α-tocopherol in counteracting OTA toxicity was evaluated in various experimental conditions using primary porcine fibroblasts. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol administration may counteract short-term OTA toxicity, thus supporting its defensive role at a cell membrane level. The second section describes how BME-UV1 was used as an in vitro model to evaluate the protective role of exogenous bovine Lf (bLf) against the cytotoxic damage induced by bacterial lipopolysaccharides (LPS). Exogenous bLf showed a protective effect against endotoxin cytotoxicity, which could be mediated by the LPS-neutralizing capability of bLf. In addition, in BME-UV1 cells the response to LPS exposure did not involve… Advisors/Committee Members: tutor: A. Baldi, coordinatore: F. Gandolfi, BALDI, ANTONELLA, GANDOLFI, FULVIO.

Subjects/Keywords: Settore AGR/18 - Nutrizione e Alimentazione Animale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saccone, F. (2013). CELL-BASED BIOASSAYS FOR TESTING BIOACTIVE COMPOUNDS IN FARM ANIMALS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/216692

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Saccone, F.. “CELL-BASED BIOASSAYS FOR TESTING BIOACTIVE COMPOUNDS IN FARM ANIMALS.” 2013. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/216692.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Saccone, F.. “CELL-BASED BIOASSAYS FOR TESTING BIOACTIVE COMPOUNDS IN FARM ANIMALS.” 2013. Web. 24 Jan 2021.

Vancouver:

Saccone F. CELL-BASED BIOASSAYS FOR TESTING BIOACTIVE COMPOUNDS IN FARM ANIMALS. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/216692.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Saccone F. CELL-BASED BIOASSAYS FOR TESTING BIOACTIVE COMPOUNDS IN FARM ANIMALS. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/216692

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. M. Campagnol. STUDIO IN VIVO DI SCAFFOLD PER LA RIGENERAZIONE OSSEA E CARTILAGINEA.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/216647

► The treatment of large osseous defects remains a challenging clinical problem in orthopedic surgery and, due to the lack of comparative studies, it is not… (more)

Subjects/Keywords: scaffold; in vivo; minipig; moc; Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Campagnol, M. (2013). STUDIO IN VIVO DI SCAFFOLD PER LA RIGENERAZIONE OSSEA E CARTILAGINEA. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/216647

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Campagnol, M.. “STUDIO IN VIVO DI SCAFFOLD PER LA RIGENERAZIONE OSSEA E CARTILAGINEA.” 2013. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/216647.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Campagnol, M.. “STUDIO IN VIVO DI SCAFFOLD PER LA RIGENERAZIONE OSSEA E CARTILAGINEA.” 2013. Web. 24 Jan 2021.

Vancouver:

Campagnol M. STUDIO IN VIVO DI SCAFFOLD PER LA RIGENERAZIONE OSSEA E CARTILAGINEA. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/216647.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Campagnol M. STUDIO IN VIVO DI SCAFFOLD PER LA RIGENERAZIONE OSSEA E CARTILAGINEA. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/216647

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. V. Serra. DEVELOPMENT OF A SEROLOGICAL TEST FOR IXODES RICINUS BITE IN VERTEBRATES.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/623311

► The research line of my PhD project was focused on parasitism supported by Ixodes ricinus tick, the most common tick species occurring in Europe infecting… (more)

▼ The research line of my PhD project was focused on parasitism supported by Ixodes ricinus tick, the most common tick species occurring in Europe infecting humans and animals. The importance of this tick species is due to its wide area of distribution (i.e. Europe and Northern Africa), to its low host specificity and to its central role in the transmission of multiple infectious agents (e.g. the causative agent of Lyme borreliosis, rickettsial diseases, tick-borne encephalitis (TBE) virus) to both humans and animals. In addition to these pathogens, I. ricinus hosts an intracellular bacterium named Midichloria mitochondrii to recall its unique intra-mitochondrial lifestyle. Indeed, this bacterium presents the peculiar capacity to invade and multiply inside mitochondria, where it has been found between the inner and outer membranes and consuming the mitochondrial matrix. The presence of M. mitochondrii has been detected in female reproductive tract, in salivary glands and rostrum of adult females of I. ricinus, where is present with a prevalence of 100%, while the prevalence in males is around 44%. A vertical transmission of the bacterium has been reported, as indicated by PCR on eggs and newly emerged larvae, together with its horizontal transmission to vertebrate host during I. ricinus blood meal, as demonstrated by molecular and serological evidences in different host species (including humans). Even though the transmission of M. mitochondrii into the vertebrate hosts during the I. ricinus bite is currently established, it is still unknown whether immature stages (larvae and nymphs) of this tick species transmit the symbiont during the blood meal. Additionally, its recently discovered replication into the vertebrate host does not necessarily imply a pathogenic role of this endosymbiont. A limit which characterizes tick parasitism in humans is represented by tick bite features: tick bites in humans often go unnoticed, because people may mistake the bite of another arthropod as that of a tick and components of tick saliva have anesthetic properties which inhibit host pain/hitch responses. Additionally, the predominant developmental stage of I. ricinus found to feed on humans are nymphs, which are difficult to detect due to their small size. For this reason, given the current lack of a reliable test for I. ricinus bite, the development of a diagnostic test for tick bite would be essential not only for the study of the clinical and epidemiological role of this vector, but also to evaluate the hypothesis of the tick bite as a risk factor for the onset of chronic degenerative diseases (Amyotrophic lateral sclerosis, rheumatologic and cardiovascular diseases). Moreover, the emergence of new tick-borne diseases and the re-emergence of existing ones, together with the rising dispersal of I. ricinus, increase the importance of this public health issue. The present PhD project has been focused on five principal researches: i) investigation of M. mitochondrii presence in blood and sera of roe deer (Capreolus capreolus)… Advisors/Committee Members: tutor: C. Bazzocchi, coordinatore: F. Gandolfi, BAZZOCCHI, CHIARA, GANDOLFI, FULVIO.

Subjects/Keywords: Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Serra, V. (2019). DEVELOPMENT OF A SEROLOGICAL TEST FOR IXODES RICINUS BITE IN VERTEBRATES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/623311

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Serra, V.. “DEVELOPMENT OF A SEROLOGICAL TEST FOR IXODES RICINUS BITE IN VERTEBRATES.” 2019. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/623311.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Serra, V.. “DEVELOPMENT OF A SEROLOGICAL TEST FOR IXODES RICINUS BITE IN VERTEBRATES.” 2019. Web. 24 Jan 2021.

Vancouver:

Serra V. DEVELOPMENT OF A SEROLOGICAL TEST FOR IXODES RICINUS BITE IN VERTEBRATES. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/623311.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Serra V. DEVELOPMENT OF A SEROLOGICAL TEST FOR IXODES RICINUS BITE IN VERTEBRATES. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/623311

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. C. Giromini. HEALTH-PROMOTING COMPOUNDS IN FOOD AND FEED: AN IN VITRO APPROACH TO STUDY DIETARY BIOACTIVES.

Degree: 2015, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/338775

► The main aim of this thesis was to study the functional properties of health-promoting compounds in vitro with an emphasis on milk proteins, vitamin E… (more)

▼ The main aim of this thesis was to study the functional properties of health-promoting compounds in vitro with an emphasis on milk proteins, vitamin E and micronutrients. The health-promoting effects of intact whey and casein proteins were evaluated after in vitro SGD. Permeate (absorbed fraction) and retentate (intestinal fraction) were obtained and used to study their health-effects in vitro. Soya protein was include as non-animal protein in all the experiments performed. Following SGD, the milk proteins exhibited antioxidant activity, ACE-inhibitory activity and tropho-functional properties at the intestinal cell level. The whey protein permeate exhibited a higher ACE-inhibitory activity compared with the casein and soya protein permeate. SGD increased the ACE-inhibitory activity of whey protein and the antioxidant activity of all the protein tested. At specific concentrations, casein, whey and soya proteins were able to modulate intestinal cell viability and the production of intestinal mucus. Moreover, the proliferation of Lactobacillus casei was increased by specific concentrations of whey and casein proteins. Modulations of mucus production and probiotic bacteria growth were observed, and casein was the primary protein that was able to stimulate MUC5AC gene expression and promote Lactobacillus casei growth. Altogether, the analyses of goblet cell proliferation and prebiotic bacterial growth may represent complementary approaches to study the bioactivities and the functions of food proteins in the gut. Additionally, the comparison of the effect of the two major intact milk proteins performed in this thesis could provide valuable information regarding which is more efficacious in improving health. α-tocopherol has been demonstrated to play an important role in reducing oxidative stress at cellular level, in different in vitro models. Food toxicants, as OTA, have been found to be able of disrupting the cell monolayer and damaging DNA, which leads to cell death. OTA reduced tight junctions protein localization in cell membranes and influenced cell-cell interactions. The immunofluorescence analyses revealed changes in the patterns of occludin and Zo1 proteins in the presence of OTA, compared with control cells. OTA cytotoxic effects were counteracted by the presence of α-tocopherol. The pre-treatment with α-tocopherol blocked the loss of occludin protein in the tight junctions of kidney cells treated with OTA. α-tocopherol supplementation has demonstrated to counteract short-term OTA toxicity at different cellular levels, supporting the defensive role of this compound in the cell membrane. Finally, the in vitro roles of different micronutrients, specifically choline/methionine and different Zn formulations, were determined. Due to their physiological health-promoting effects, both choline/methionine and Zn are commonly used in animal nutrition. The results of this thesis confirmed that under condition of stress, choline and methionine have important roles in enhancing cell viability and counteracting oxidative… Advisors/Committee Members: tutor: A. Baldi, coordinatore: F. Gandolfi, BALDI, ANTONELLA, GANDOLFI, FULVIO.

Subjects/Keywords: Settore AGR/18 - Nutrizione e Alimentazione Animale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Giromini, C. (2015). HEALTH-PROMOTING COMPOUNDS IN FOOD AND FEED: AN IN VITRO APPROACH TO STUDY DIETARY BIOACTIVES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/338775

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Giromini, C.. “HEALTH-PROMOTING COMPOUNDS IN FOOD AND FEED: AN IN VITRO APPROACH TO STUDY DIETARY BIOACTIVES.” 2015. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/338775.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Giromini, C.. “HEALTH-PROMOTING COMPOUNDS IN FOOD AND FEED: AN IN VITRO APPROACH TO STUDY DIETARY BIOACTIVES.” 2015. Web. 24 Jan 2021.

Vancouver:

Giromini C. HEALTH-PROMOTING COMPOUNDS IN FOOD AND FEED: AN IN VITRO APPROACH TO STUDY DIETARY BIOACTIVES. [Internet] [Thesis]. Università degli Studi di Milano; 2015. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/338775.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Giromini C. HEALTH-PROMOTING COMPOUNDS IN FOOD AND FEED: AN IN VITRO APPROACH TO STUDY DIETARY BIOACTIVES. [Thesis]. Università degli Studi di Milano; 2015. Available from: http://hdl.handle.net/2434/338775

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. M. Ottoboni. INNOVATION TECHNOLOGY IN FEED FORMULATION AND PRODUCTION.

Degree: 2015, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/338778

► The general aim of this thesis was to investigate new technology in feed formulation and production. For this purpose several aspects in the field of… (more)

▼ The general aim of this thesis was to investigate new technology in feed formulation and production. For this purpose several aspects in the field of feed production were investigated. Specifically, during the course of this PhD program, three main sub aims have been addressed: i) evaluation of new ingredients, i.e., insect materials, in feed formulation and the impact of feed technology on their nutritional value; ii) improvements in the official methods for detecting Processed Animal Proteins (PAPs), and iii) investigation of the innovation, research, and development needs in in the feed industry in two selected areas. With respect to the first sub aim, fresh insect (Hermetia illucens) material was evaluated as an alternative protein source in experimental feed. Differents experimental formulae containing insect material were tested under differing extrusion conditions. Extruded feed was also evaluated for digestibility. The results indicate that fresh Hermetia illucens material can be efficiently included in experimental extruded feed containing 25% insect material and 75% wheat. Technological treatment, i.e., extrusion, increased in vitro organic matter digestibility, and did not affect protein digestibility. With regard to the second sub-aim the basic assumption was that insect material, if authorized, should be considered as animal material. Accordingly, the second sub aim of the thesis focused on implementing existing methods for processing animal protein and investigating their potential in tracing and characterizing insect material. In this scenario, experiments were aimed toward improving the official microscopy techniques for detecting processed animal proteins (PAP) by combining those with image analysis (IA) technology. The studies conducted aim to i) characterize fish meal material in compound feed (i.e., aquafeed), ii) identify specific selected markers able to efficiently distinguish between fish and terrestrial materials, iii) distinguish between mammalian materials, and iv) verify the applicability of the method for identifying insect material in feed. The results obtained in this context indicated that even though microscopy seems to be a promising approach for identifying both animal proteins and insect material, using microscopy alone has some limitations; therefore, a combined approach with other methods (i.e., PCR) is recommended. With regard to the third sub aim, research and development needs and innovation in the feed industry, the results of a targeted survey conducted in two countries (Italy and Serbia) showed that innovation in raw materials is a key factor for large multinational industries. In contrast, the survey results obtained from small and medium feed companies are quite different; for these companies, cost reduction, decreased energy consumption, improved quality, improved market image, development of new markets and satisfying market demand are much more important. By combining the results obtained from the various studies described in this paper, it can be concluded… Advisors/Committee Members: tutor: F. Cheli, coordinatore: F. Gandolfi, CHELI, FEDERICA, GANDOLFI, FULVIO.

Subjects/Keywords: BSF; insect; feed technology; digestibility; extrusion; image analysis; microscopy; processed animal proteins (PAPs); feedneeds; research and development; feed industry; Settore AGR/18 - Nutrizione e Alimentazione Animale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ottoboni, M. (2015). INNOVATION TECHNOLOGY IN FEED FORMULATION AND PRODUCTION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/338778

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ottoboni, M.. “INNOVATION TECHNOLOGY IN FEED FORMULATION AND PRODUCTION.” 2015. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/338778.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ottoboni, M.. “INNOVATION TECHNOLOGY IN FEED FORMULATION AND PRODUCTION.” 2015. Web. 24 Jan 2021.

Vancouver:

Ottoboni M. INNOVATION TECHNOLOGY IN FEED FORMULATION AND PRODUCTION. [Internet] [Thesis]. Università degli Studi di Milano; 2015. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/338778.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ottoboni M. INNOVATION TECHNOLOGY IN FEED FORMULATION AND PRODUCTION. [Thesis]. Università degli Studi di Milano; 2015. Available from: http://hdl.handle.net/2434/338778

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. M. Albonico. IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/489075

► Mycotoxins, secondary metabolites produced by moulds, mainly Aspergillus spp., Fusarium spp. and Penicillium spp., are common contaminants of food and feed. The aim of this… (more)

▼ Mycotoxins, secondary metabolites produced by moulds, mainly Aspergillus spp., Fusarium spp. and Penicillium spp., are common contaminants of food and feed. The aim of this project was to evaluate: (i) the potential endocrine disruptor effects of fumonisin B1 (FB1), beauvericin (BEA), deoxynivalenol (DON) and zearalenone (ZEA) metabolites �-zearalenol (�-ZEA) and �zearalenol (�ZEA), alone and combined, using a bovine granulosa cell (GC) in vitro model and (ii) the individual and combined effects of FB1 and BEA on the intestinal barrier using Caco-2 cells cultured in vitro on semipermeable inserts. The results obtained indicated that FB1 alone at all tested doses (0; 0.5; 1; 1.5; 3; 6 �M) had no effects on GC proliferation and progesterone (P4) production. In the presence of �ZEA at30 ng/mL (0.094 �M), FB1 at 30 ng/mL (0.042 �M) showed a stimulatory effect on GC numbers. Cell proliferation decreased after exposure to �ZEA alone at 5.0 mg/mL (15.6 �M) and FB1 with �-ZEA and �ZEA at the same concentration. Regarding steroid production, FB1 at 30 ng/mL (0.042 �M ) and 100 ng/mL (0.13 �M amplified the inhibitory effect of �ZEA at 30 ng/mL (0.094 �M) on estradiol (E2) production, while FB1 alone increased (P<0.05) IGF1-induced E2 production. FB1 in combination with �ZEA decreased (P < 0.05) E2 production. FB1 at 1, 1.5 and 3 �M slightly inhibited (P < 0.05) E2 production. BEA at concentrations � 3 �M was found to strongly decrease (P < 0.05) both steroid production and FB1 did not influence the effects of BEA. At 10 �M both mycotoxins decreased (P < 0.001) serum-induced GC proliferation. At 30 �M, BEA showed inhibitory effects on FSH plus IGF-1-induced CYP11A1 and CYP19A1 mRNA abundance (P < 0.05), whereas FB1 at 30 �M had no effect on CYP11A1 and CYP19A1 gene expression. As regards the effects of FB1 and BEA, alone and combined, on the Caco-2 intestinal barrier model data showed a TEER decrease after 1 h and 2 h of Bl exposure to BEA at 0.5 and 1.5 �M and after 24 h of Bl exposure to BEA at 0.5 �M, whereas after 24 h of Bl exposure, BEA at 3 and 6 �M was found to significantly (P < 0.05) increase TEER. FB1 had no effect on the intestinal barrier integrity and when combined with BEA the TEER increase induced by BEA was no longer observed. Cytokine release was observed only after exposure to BEA alone, and not in combination with FB1, with an increase of IL-6 and IL8 release after apical exposure to 3 and 6 �M and after basolateral exposure to 1.5, 3, 6 �M for IL-6 and only to 6 �M for IL-8. TNF- � release was induced by Ap (0.5 -1.5 �M) and Bl (1.5 �M) exposure to BEA. Overall, these results provide information on in vitro toxicological effects of Fusarium mycotoxins. Advisors/Committee Members: tutor: F. Caloni, coordinatore: F. Gandolfi, CALONI, FRANCESCA, GANDOLFI, FULVIO.

Subjects/Keywords: Mycotoxins; Fusarium; granulosa cells; Caco-2 cells; fumonisin; beauvericin; Settore VET/07 - Farmacologia e Tossicologia Veterinaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Albonico, M. (2017). IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/489075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Albonico, M.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS.” 2017. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/489075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Albonico, M.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS.” 2017. Web. 24 Jan 2021.

Vancouver:

Albonico M. IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/489075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Albonico M. IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/489075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. M. Cozzi. FLOW CYTOMETRY FOR THE DIAGNOSIS AND THE CHARACTERIZATION OF CANINE LYMPHOPROLIPHERATIVE TUMORS.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/580991

► ABSTRACT Flow cytometry (FC) is a diagnostic technique in continuous development and, to date, it plays a fundamental role in human medicine for the diagnosis… (more)

▼ ABSTRACT Flow cytometry (FC) is a diagnostic technique in continuous development and, to date, it plays a fundamental role in human medicine for the diagnosis and the classification of hematopoietic tumors. Several studies state that the information obtained from the FC analysis in addition to defining the immunophenotype of the tumor cells, hence the origin of the neoplasia, can help to predict its biological behavior (Swerdlow et al., 2016). FC has thus become a simple and objective method to characterize hematopoietic tumors in order to obtain also valuable prognostic information. In veterinary medicine, FC is increasingly adopted for the diagnosis, staging, and monitoring of hematopoietic tumors, but despite advances in the generation and validation of antibodies for the use in dogs, the characterization of such neoplasms remains challenging (Wilkerson et al., 2005; Comazzi and Gelain, 2011). The aim of this doctoral project is to describe some less frequent subtypes of lymphomas and leukemia of the dog via FC, in order to define its biological behavior and to investigate whether there is any variable of prognostic value among all the factors analyzed. For this purpose, four studies will be illustrated; the first is a retrospective work, aiming to evaluate pre-analytical factors that may affect the diagnostic utility of FC in samples of lymph node aspirates. The work included 987 cases selected in the period 2009-2015, in which a lymph node aspirate was sent to our laboratory with suspect of lymphoproliferative disease. In order to define any possible bias affecting the outcome of the FC diagnosis, the variables analyzed were related to the animal (breed, sex, age), related to the operator (year, season, method of delivery to the laboratory, referring veterinarian) and related to the sample (type of material, cell concentration, presence of cytological slides, presence of artifacts). Of the factors considered, the sample cellularity and the presence of dead cells were the ones that most influenced the possibility of obtaining an adequate diagnosis. FC was, however, conclusive in almost all the samples, that were characterized by good quality and adequate sampling conditions. The study focused on TZL, a peculiar canine lymphoma with an indolent behavior, aimed to characterize this entity from a clinical and pathological point of view. The first phase of the work was retrospective, with the aim of describing clinical presentation and outcome of 51 cases selected between 2009 and 2014. The second phase of the study was aimed at clarifying the origin of the peculiar CD45 negative T-immunophenotype (Martini et al., 2013; Seelig et al., 2014); specifically, we confirmed the absence of the surface protein by means of two different techniques (flow cytometry and immunohistochemistry) and verified whether the transcript and the gene encoding the protein were present. The results confirmed that this type of lymphoma has indolent behavior with long survival times, despite being often diagnosed at the V stage of the… Advisors/Committee Members: tutor: S. Comazzi, coordinatore: F. Gandolfi, COMAZZI, STEFANO, GANDOLFI, FULVIO.

Subjects/Keywords: Flow Cytometry; Canine; Tumor; Lymphoma; Leukemia; Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cozzi, M. (2018). FLOW CYTOMETRY FOR THE DIAGNOSIS AND THE CHARACTERIZATION OF CANINE LYMPHOPROLIPHERATIVE TUMORS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/580991

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cozzi, M.. “FLOW CYTOMETRY FOR THE DIAGNOSIS AND THE CHARACTERIZATION OF CANINE LYMPHOPROLIPHERATIVE TUMORS.” 2018. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/580991.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cozzi, M.. “FLOW CYTOMETRY FOR THE DIAGNOSIS AND THE CHARACTERIZATION OF CANINE LYMPHOPROLIPHERATIVE TUMORS.” 2018. Web. 24 Jan 2021.

Vancouver:

Cozzi M. FLOW CYTOMETRY FOR THE DIAGNOSIS AND THE CHARACTERIZATION OF CANINE LYMPHOPROLIPHERATIVE TUMORS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/580991.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cozzi M. FLOW CYTOMETRY FOR THE DIAGNOSIS AND THE CHARACTERIZATION OF CANINE LYMPHOPROLIPHERATIVE TUMORS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/580991

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. F. Maghin. NATURAL EXTRACTS IN ANIMAL NUTRITION: ANIMAL WELL¿BEING AND PRODUCTS QUALITY.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/488996

► The main objective of this thesis was to have a deeper knowledge about the influence of feeding strategies with several natural plants extracts on pig… (more)

▼ The main objective of this thesis was to have a deeper knowledge about the influence of feeding strategies with several natural plants extracts on pig performance and meat quality in different phases of pig production. To achieve this objective, a set of 4 studies were performed (chapter 2-6). The first study evaluated the effect of dietary supplementation with a mixture of algae plus polyphenols (AM) in lactating sow on maternal and piglets performance and sows reproductive data until the subsequent farrowing. This study showed that integration of AM in sow diet decreased the fat mobilization during lactation. Moreover, dietary AM inclusion in lactating sows positively affected body weight and average daily gain in piglets at 21 days. Moreover, sows that received AM had more total number of piglets born at the subsequent farrowing. Regarding the effects of plant extracts on meat and product quality, two different studies were performed. In the second study of the thesis, the impact of dietary integration with vitamin E and verbascoside (AOX) in pigs, on carcass characteristics, meat quality, shelf life of pork under modified atmosphere packaging (MAP) was evaluated. This study demonstrated the positive effects of plant extracts as antioxidant. Particularly, highlighted that dietary AOX positively affected carcass dressing percentage and pork oxidative and colour stability. Moreover, sensory analysis revealed that, at 15 days under MAP, meat from AOX was comparable with fresh meat in appearance and aroma. In the same way, the third study showed that vitamin E and verbascoside (AOX) also affected the quality of derived product like smoked cured ham. Results relieved infact that the AOX dietary inclusion affected the seasoning losses and influenced the consumers' preference of smoked cured ham, without affecting other quality parameters. Finally, the fourth experimental trial examined the impact of 3 % hydrolysable tannins (HT) from chestnut extract and two levels of polyunsaturated fatty acid (PUFA) in the diets on growth performance, carcass traits, meat quality and boar taint compounds in entire males. Results showed that dietary HT reduced the feed efficiency but not feed intake and the pigs final weight. These results show that performance, carcass composition and meat quality traits are not affected by dietary 3% chestnut extract supplementation in entire male. The boar taints compounds like androstenone, skatole and indole tended to be lower in HT group. The cytochrome mRNA expression in the liver and colon mucosa was not affected by the diet. No evident relationship between dietary PUFA level and boar taint compound levels was observed. Overall, these results contribute to improve the knowledge regarding beneficial effects of plant extracts. The inclusion of bioactive components contained in natural extracts can be considered an innovative approach to improve pig wellbeing and pork quality without negative effects on animal production. Advisors/Committee Members: tutor: C. Corino, coordinatore: F. Gandolfi, CORINO, CARLO, GANDOLFI, FULVIO.

Subjects/Keywords: Swine; Pig production; Natural extracts; Pig performance; Meat quality; Settore AGR/18 - Nutrizione e Alimentazione Animale

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APA (6^th Edition):

Maghin, F. (2017). NATURAL EXTRACTS IN ANIMAL NUTRITION: ANIMAL WELL¿BEING AND PRODUCTS QUALITY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/488996

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maghin, F.. “NATURAL EXTRACTS IN ANIMAL NUTRITION: ANIMAL WELL¿BEING AND PRODUCTS QUALITY.” 2017. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/488996.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maghin, F.. “NATURAL EXTRACTS IN ANIMAL NUTRITION: ANIMAL WELL¿BEING AND PRODUCTS QUALITY.” 2017. Web. 24 Jan 2021.

Vancouver:

Maghin F. NATURAL EXTRACTS IN ANIMAL NUTRITION: ANIMAL WELL¿BEING AND PRODUCTS QUALITY. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/488996.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maghin F. NATURAL EXTRACTS IN ANIMAL NUTRITION: ANIMAL WELL¿BEING AND PRODUCTS QUALITY. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/488996

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. R.T.M.M. Prinsen. CNV DETECTION AND ASSOCIATION STUDIES IN THE BROWN SWISS CATTLE BREED.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/485105

► Sintesi – Italiano Gli scopi, i materiali, i metodi usati, i risultati e le conclusioni dei tre studi sono organizzati in tre capitoli. La sintesi… (more)

Subjects/Keywords: Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico

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APA (6^th Edition):

Prinsen, R. (2017). CNV DETECTION AND ASSOCIATION STUDIES IN THE BROWN SWISS CATTLE BREED. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/485105

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prinsen, R.T.M.M.. “CNV DETECTION AND ASSOCIATION STUDIES IN THE BROWN SWISS CATTLE BREED.” 2017. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/485105.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prinsen, R.T.M.M.. “CNV DETECTION AND ASSOCIATION STUDIES IN THE BROWN SWISS CATTLE BREED.” 2017. Web. 24 Jan 2021.

Vancouver:

Prinsen R. CNV DETECTION AND ASSOCIATION STUDIES IN THE BROWN SWISS CATTLE BREED. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/485105.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prinsen R. CNV DETECTION AND ASSOCIATION STUDIES IN THE BROWN SWISS CATTLE BREED. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/485105

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. A. Cafiso. EMERGING PATHOGENS IN VERTEBRATES: BIOLOGY, GENOMICS AND INFECTIVITY OF BACTERIA ASCRIBED TO THE MIDICHLORIACEAE FAMILY.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/486684

► This PhD thesis is the outcome of a wider project focused on studying the biology, genomics and infectivity of bacteria ascribed to the Midichloriaceae family.… (more)

▼ This PhD thesis is the outcome of a wider project focused on studying the biology, genomics and infectivity of bacteria ascribed to the Midichloriaceae family. Midichloriaceae are a group of bacteria extremely diversified in terms of the ecological and taxonomic diversity of their hosts. Members of the family Midichloriaceae can be considered as a wide bacterial group, still to be investigated under several aspects. In particular, the association of members of the family Midichloriaceae with parasites (such as ticks, fleas and parasitic amoebae) suggests that these bacteria could be potentially infectious to vertebrates (including humans) and even responsible for medical and veterinarian diseases. The researchers conducted in this PhD project have been focused on three main topics: i) possible involvement of a midichloriacea in a disease (red mark syndrome; RMS) affecting rainbow trout; ii) in-depth analysis of different aspects concerning the hard tick Ixodes ricinus and its intramitochondrial symbiont Midichloria mitochondrii (i.e. antibiotic treatment of the arthropod, salivary glands vs. ovaric tissue comparative proteomics, transmission of the symbiont to the vertebrate host); iii) presence of M. mitochondrii-related bacteria in different tick species. A brief description of each topic of the work done is here reported: i) RMS is a chronic skin disease of unknown etiology affecting farmed rainbow trout Oncorhynchus mykiss in Europe. It consists of single or multiple skin lesions usually localized on the trunk of fish approaching market size. Many authors suppose that a bacterial infection could be the cause of the disease. Some studies have reported a possible correlation between the presence of a Rickettsia-like organism (subsequently ascribed to the family Midichloriaceae) and RMS skin lesions. Our work concerning the possible involvement of a midichloriacea in RMS in rainbow trout resulted in the detection of bacteria of this family, besides in skin lesions, in various internal organs and in the bloodstream. No bacteria were found in unaffected fish and in healthy skin samples from affected individuals. Bacteria were detected also in fish ponds water were RMS-affected fish are farmed. These results give support to the hypothesis of the involvement of these bacteria in developing RMS. It is possible that some free-living eukaryotes could host or be the vector of this midichloriacea. ii) M. mitochondrii is the most prevalent symbiont of the hard tick I. ricinus, present in 100% of eggs and adult females of wild I. ricinus populations. This bacterium is intracellular and is the only known symbiont able to invade the mitochondria of the host cells. However, the role that M. mitochondrii plays in the host metabolism has yet to be elucidated. In addition, multiple lines of evidence indicate the possibility of the transmission of this bacterium to the vertebrate host during the tick blood meal. In order to investigate the role of M. mitochondrii in the biology of the tick host, we performed an antibiotic… Advisors/Committee Members: tutor: C. Bazzocchi, coordinatore: F. Gandolfi, BAZZOCCHI, CHIARA, GANDOLFI, FULVIO.

Subjects/Keywords: midichloria spp.; midichloria mitochondrii; symbiosis; tick; ixodes ricinus; transmission; red mark syndrome; oncorhynchus mykiss; midichloriaceae; Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cafiso, A. (2017). EMERGING PATHOGENS IN VERTEBRATES: BIOLOGY, GENOMICS AND INFECTIVITY OF BACTERIA ASCRIBED TO THE MIDICHLORIACEAE FAMILY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/486684

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cafiso, A.. “EMERGING PATHOGENS IN VERTEBRATES: BIOLOGY, GENOMICS AND INFECTIVITY OF BACTERIA ASCRIBED TO THE MIDICHLORIACEAE FAMILY.” 2017. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/486684.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cafiso, A.. “EMERGING PATHOGENS IN VERTEBRATES: BIOLOGY, GENOMICS AND INFECTIVITY OF BACTERIA ASCRIBED TO THE MIDICHLORIACEAE FAMILY.” 2017. Web. 24 Jan 2021.

Vancouver:

Cafiso A. EMERGING PATHOGENS IN VERTEBRATES: BIOLOGY, GENOMICS AND INFECTIVITY OF BACTERIA ASCRIBED TO THE MIDICHLORIACEAE FAMILY. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/486684.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cafiso A. EMERGING PATHOGENS IN VERTEBRATES: BIOLOGY, GENOMICS AND INFECTIVITY OF BACTERIA ASCRIBED TO THE MIDICHLORIACEAE FAMILY. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/486684

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. S. Frattini. EPIGENETIC INVESTIGATION ON CYCLIC ONSET OF REPRODUCTIVE ACTIVITY IN DAIRY GOATS.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/487065

► At our latitude and under natural conditions, goats show autumn heats with calvings in spring. Individual variability in the onset of puberty and reproductive activity… (more)

▼ At our latitude and under natural conditions, goats show autumn heats with calvings in spring. Individual variability in the onset of puberty and reproductive activity could hide a genetic and epigenetic variability that can allow a selection of goats more prone to deseasonalization. As a consequence, breeders could easily spread milk production throughout the year. The aim of the project was to analyze epigenetic profiles responsible for the reproductive seasonality in goat species. Considering the lack of information in literature for the species, we decided, first of all, to focus our effort on the characterization of the epigenetic profile (involving DNA methylation and microRNA) of two key organ in reproduction performances: hypothalamus and ovary. This basic research gave a first picture of the epigenome of goat species, representing a starting point for future researches in this field. In the three year of my PhD we gathered biological samples of interest for the project and we set up a pipeline for the epigenetic analysis (for DNA methylation and smallRNA) of the data produced. We saw different DNA methylation patterns across the goat genome between two organs, hypothalamus and ovary, and even a marked difference in the same organ (hypothalamus) between two physiological stages, before and after reaching puberty. In particular, the comparison between transcriptome and methylome in hypothalamus and ovary showed that a higher level of methylation is not accompanied by a higher gene suppression. We discovered interesting differences in the expression of microRNA in hypothalamus and ovary. We confirmed microRNAs already known on databases and we discovered some new, through structure homology with other related species. A selection of differentially expressed miRNAs was identified among hypothalamus, pituitary and ovary. In addition, 44, 55, and 69 miRNAs identified in pituitary, hypothalamus and ovary respectively, were specifically up-regulated in only one organ. Then we focused our efforts on the characterization of the epigenetic factors that could drive to puberty in the species. Genome wide DNA methylation analysis revealed 664 Differentially Methylated Genes (DMGs) among pubertal goats and their control. The crossing of DMGs and Differentially Expressed Genes (DEGs) among pubescent goats and their control, revealed new 8 genes, as potential marker for puberty onset. Among these, ATG16L1 (autophagy related 16 like 1), LRP5 (low-density lipoprotein receptor-related protein 5) and MERTK (proto-oncogene tyrosine-protein kinase MER) are suitable examples of puberty-related genes. According to False Discovery Rate threshold, it seems that miRNA population does not play a crucial role in the reaching of puberty in goat species in the organ analyzed. Furthermore, we conducted a SNP discovery on three key genes for photoperiod response (Melatonin Receptor 1 - MTNR1A, type 2 Deiodinase Gene - DIO2, Thyroid Stimulating Hormone beta subunit - TSHB), confirming and identifying new genetic polymorphisms. We found a… Advisors/Committee Members: tutor: G. Pagnacco, coordinatore: F. Gandolfi, PAGNACCO, GIULIO GIORGIO ALESSANDRO.

Subjects/Keywords: Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frattini, S. (2017). EPIGENETIC INVESTIGATION ON CYCLIC ONSET OF REPRODUCTIVE ACTIVITY IN DAIRY GOATS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/487065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Frattini, S.. “EPIGENETIC INVESTIGATION ON CYCLIC ONSET OF REPRODUCTIVE ACTIVITY IN DAIRY GOATS.” 2017. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/487065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Frattini, S.. “EPIGENETIC INVESTIGATION ON CYCLIC ONSET OF REPRODUCTIVE ACTIVITY IN DAIRY GOATS.” 2017. Web. 24 Jan 2021.

Vancouver:

Frattini S. EPIGENETIC INVESTIGATION ON CYCLIC ONSET OF REPRODUCTIVE ACTIVITY IN DAIRY GOATS. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/487065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Frattini S. EPIGENETIC INVESTIGATION ON CYCLIC ONSET OF REPRODUCTIVE ACTIVITY IN DAIRY GOATS. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/487065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. A.F. TOMAS MARQUES. MOLECULAR BIOMARKERS OF WELFARE IN POULTRY.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/485613

► La tesi si propone di esplorare il rapporto tra stress e risposta immunitaria nel pollame, concentrandosi su proteine di fase acuta (APPs) e microRNA (miRNAs)… (more)

Subjects/Keywords: biomarkers; welfare; poultry; Meleagris gallopavo; Gallus gallus; Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria

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APA (6^th Edition):

MARQUES, A. T. (2017). MOLECULAR BIOMARKERS OF WELFARE IN POULTRY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/485613

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

MARQUES, A.F. TOMAS. “MOLECULAR BIOMARKERS OF WELFARE IN POULTRY.” 2017. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/485613.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

MARQUES, A.F. TOMAS. “MOLECULAR BIOMARKERS OF WELFARE IN POULTRY.” 2017. Web. 24 Jan 2021.

Vancouver:

MARQUES AT. MOLECULAR BIOMARKERS OF WELFARE IN POULTRY. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/485613.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

MARQUES AT. MOLECULAR BIOMARKERS OF WELFARE IN POULTRY. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/485613

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. M. Nobile. DEVELOPMENT AND VALIDATION OF METHODS FOR THE DETECTION OF RESIDUES IN UNCONVENTIONAL AND INNOVATIVE MATRICES THROUGH LC-MS/MS ANALYSES FOR SAFETY OF FOOD OF ANIMAL ORIGIN.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/547683

► Successful animal growth depends on a combination of many factors related to health, management and nutrition. The use of veterinary drugs in food-producing animals for… (more)

▼ Successful animal growth depends on a combination of many factors related to health, management and nutrition. The use of veterinary drugs in food-producing animals for therapeutic purposes is regulated (corticosteroids, antibiotics) or banned (anabolic steroids) in the European Union; however, their use as growth promoters cannot be excluded. Moreover, the eventual presence of residues in food constitutes a fraud and a health issue for the consumers. For these reasons the need to find new accumulation matrices and new sensitive, specific and robust methods that are able to reveal the presence of drug residues is essential, based on the fact that there is a low percentage of non-conformity in the final reports of the National Residues Plan in recent years, although the threat of a disproportionate use of these substances is increasingly on the rise. In the light of these facts, there is the need to implement the framework of controls aimed to food safety, due to the inefficiency of tools for the study of these substances. Often, the use of conventional matrices, such as urine, liver or muscle, recommended for the official controls of illegal treatment are not completely satisfactory due to the fast elimination rate of the compounds or to the difficulties arising from the compounds characterised also by a pseudoendogenous nature. The debate about the presence of β-boldenone II phase metabolites and prednisolone in urine samples, owing to endogenous or illicit treatment, is currently ongoing within the European Union. These compounds have been appropriately defined “grey-zone substances”, for their double origin. The simple detection of some steroids in urine is currently considered to provide insufficient evidence of illicit treatment. Parameters such as cut-off levels, the presence of metabolites, or both, must be accounted for. As regards antibiotics, the overuse, over the last decades, as growth promoters in food producing animal have caused favorable condition about the threat of bacterial resistance. The antibiotics can directly affect the consumer in the form of residues from the food chain, or by accumulation in the environment via the application of manure to land as organic fertiliser, via sludge storage or by direct contamination of illicitly additivated water and feed. The main challenge is to monitor contemporally different antibiotic classes, in different steps of the food chain, trying to control this phenomenon. On the other hand, food contamination by new environmental contaminants should not be neglected. In particular, perfluoroalkyl substances (PFASs) have recently aroused great scientific interest and concern for public health, due to the fact they have been found in appreciable concentrations in human serum. On the basis of EFSA requestes and of analytical problems associated with their determination many studies are recommended to monitor their presence, building a database on PFASs in food, evaluate the contamination levels of the individual compound and finally draw up a reliable risk… Advisors/Committee Members: tutor: L.M. Chiesa, coordinatore: F. Gandolfi, CHIESA, LUCA MARIA, GANDOLFI, FULVIO.

Subjects/Keywords: Settore VET/04 - Ispezione degli Alimenti di Origine Animale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nobile, M. (2018). DEVELOPMENT AND VALIDATION OF METHODS FOR THE DETECTION OF RESIDUES IN UNCONVENTIONAL AND INNOVATIVE MATRICES THROUGH LC-MS/MS ANALYSES FOR SAFETY OF FOOD OF ANIMAL ORIGIN. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/547683

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nobile, M.. “DEVELOPMENT AND VALIDATION OF METHODS FOR THE DETECTION OF RESIDUES IN UNCONVENTIONAL AND INNOVATIVE MATRICES THROUGH LC-MS/MS ANALYSES FOR SAFETY OF FOOD OF ANIMAL ORIGIN.” 2018. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/547683.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nobile, M.. “DEVELOPMENT AND VALIDATION OF METHODS FOR THE DETECTION OF RESIDUES IN UNCONVENTIONAL AND INNOVATIVE MATRICES THROUGH LC-MS/MS ANALYSES FOR SAFETY OF FOOD OF ANIMAL ORIGIN.” 2018. Web. 24 Jan 2021.

Vancouver:

Nobile M. DEVELOPMENT AND VALIDATION OF METHODS FOR THE DETECTION OF RESIDUES IN UNCONVENTIONAL AND INNOVATIVE MATRICES THROUGH LC-MS/MS ANALYSES FOR SAFETY OF FOOD OF ANIMAL ORIGIN. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/547683.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nobile M. DEVELOPMENT AND VALIDATION OF METHODS FOR THE DETECTION OF RESIDUES IN UNCONVENTIONAL AND INNOVATIVE MATRICES THROUGH LC-MS/MS ANALYSES FOR SAFETY OF FOOD OF ANIMAL ORIGIN. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/547683

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. G.F. Labella. A SPREAD STUDY ON THE PRESENCE, IN DIFFERENT ANIMAL MATRICES, OF RESIDUES OF DIFFERENT ORIGIN: PSEUDO-ENDOGENOUS SUBSTANCES AND ENVIRONMENTAL CONTAMINANTS.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/547681

► The presence of xenobiotic residues in food of animal origin represents an issue for both producers and consumers. Many are the classes of substances, which… (more)

▼ The presence of xenobiotic residues in food of animal origin represents an issue for both producers and consumers. Many are the classes of substances, which could be present as residues; the most important are veterinary drugs, substances having anabolic effects, or those not authorized, and environmental contaminants. Several European and National legislations are available with the aim of proposing monitoring plans and maximum residue levels. Over the years, new substances become the subject of Control Authorities, which require the development of state-of-the-art methods for the detection of these compounds and, where necessary, the evaluations of their occurrence and the related risk for the consumer’s health. Based on these considerations, this PhD thesis is focused on the development and validation of new analytical methods for the analyses of these compounds in different matrices of animal origin, considering that innovative and sophisticated techniques are always required in order to investigate their presence. The first part of the project is focused on two “pseudo-endogenous”: prednisolone investigated in urine and adrenal gland of pigs and thiouracil in urine and thyroid gland of cows. Concerning prednisolone, it was detected in urine both at the farm and at the slaughterhouse, with a concentration and frequency higher at slaughter, while in the adrenal glands it was detected in 89% of the samples. Regarding thiouracil, and for other thyreostatic drugs, two simple methods without the derivatisation step were developed for their analyses in both cow urine and in thyroid glands. The validated methods showed satisfactory results for the recovery (96–104 % for both the matrices), precision (coefficients of variation were less than 20 % for urine and 21 % for thyroid glands). The decision limit and detection capability for all the compounds were lower than the recommended values. In urine, the decision limit ranged from 6.9 to 7.3 μg L−1, and the detection capability from 8.5 to 9.7 μg L−1, while in thyroid glands these values varied from 6.6 μg kg−1 to 7.4 μg kg−1 and from 8.0 μg kg−1 to 9.7 μg kg−1, respectively. The second part of the project takes in consideration the presence of environmental contaminants in food of animal origin (in particular fish and honey). The first study was focused on the evaluation of the distribution of persistent organic pollutants (POPs) in tuna samples (Thunnus thynnus) from different FAO areas. The results obtained showed that POPs contamination of tuna reflects FAO area contamination, in particular for FAO area 37, Mediterranean and Black seas, which is an enclosed bacin, with heavily populated shores. The second study assessed the occurrence of different classes of contaminants in 59 organic honeys. Residues of many contaminants were found in most of the samples investigated. The majority of honey samples contained at least one of the contaminants, even if their concentrations were found to be lower than its maximum residue level (MRL).… Advisors/Committee Members: tutor: F. Arioli, coordinatore. F. Gandolfi, ARIOLI, FRANCESCO, GANDOLFI, FULVIO.

Subjects/Keywords: Settore VET/07 - Farmacologia e Tossicologia Veterinaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Labella, G. (2018). A SPREAD STUDY ON THE PRESENCE, IN DIFFERENT ANIMAL MATRICES, OF RESIDUES OF DIFFERENT ORIGIN: PSEUDO-ENDOGENOUS SUBSTANCES AND ENVIRONMENTAL CONTAMINANTS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/547681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Labella, G.F.. “A SPREAD STUDY ON THE PRESENCE, IN DIFFERENT ANIMAL MATRICES, OF RESIDUES OF DIFFERENT ORIGIN: PSEUDO-ENDOGENOUS SUBSTANCES AND ENVIRONMENTAL CONTAMINANTS.” 2018. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/547681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Labella, G.F.. “A SPREAD STUDY ON THE PRESENCE, IN DIFFERENT ANIMAL MATRICES, OF RESIDUES OF DIFFERENT ORIGIN: PSEUDO-ENDOGENOUS SUBSTANCES AND ENVIRONMENTAL CONTAMINANTS.” 2018. Web. 24 Jan 2021.

Vancouver:

Labella G. A SPREAD STUDY ON THE PRESENCE, IN DIFFERENT ANIMAL MATRICES, OF RESIDUES OF DIFFERENT ORIGIN: PSEUDO-ENDOGENOUS SUBSTANCES AND ENVIRONMENTAL CONTAMINANTS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/547681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Labella G. A SPREAD STUDY ON THE PRESENCE, IN DIFFERENT ANIMAL MATRICES, OF RESIDUES OF DIFFERENT ORIGIN: PSEUDO-ENDOGENOUS SUBSTANCES AND ENVIRONMENTAL CONTAMINANTS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/547681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. G. Farina. Use of functional fatty acids in animal nutrition to improve animal health status and derived products.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/580291

► Lo scopo della presente ricerca è stato quello di valutare degli effetti della somministrazione di differenti fonti di acidi grassi sulla salute animale e sulla… (more)

Subjects/Keywords: POLYUSATURATED FATTY ACIDS; GOATS; POULTRY; GENE EXPRESSION; EGG QUALITY; Settore AGR/18 - Nutrizione e Alimentazione Animale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Farina, G. (2018). Use of functional fatty acids in animal nutrition to improve animal health status and derived products. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/580291

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Farina, G.. “Use of functional fatty acids in animal nutrition to improve animal health status and derived products.” 2018. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/580291.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Farina, G.. “Use of functional fatty acids in animal nutrition to improve animal health status and derived products.” 2018. Web. 24 Jan 2021.

Vancouver:

Farina G. Use of functional fatty acids in animal nutrition to improve animal health status and derived products. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/580291.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Farina G. Use of functional fatty acids in animal nutrition to improve animal health status and derived products. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/580291

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. E. Mariani. NUTRITION AS A TOOL TO MODULATE PRODUCTION ANIMAL HEALTH.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/626377

► Nutrition plays an important role in maintaining a good health status of animals as nutrients can modulate oxidative status, immune and inflammatory response. Moreover, there… (more)

▼ Nutrition plays an important role in maintaining a good health status of animals as nutrients can modulate oxidative status, immune and inflammatory response. Moreover, there is a need of innovative additives and feeds that can improve organism defences against diseases. The aim of this work was to monitor different markers of oxidative stress and inflammation in production animals in response to different feed additives. In this study, first, the activation of Nuclear factor-E2 related factor 2 in transgenic mice fed different sources and amount of fatty acids was monitored. Inducing the transcription of genes involved in oxidative stress response, Nrf2 represents one of the main actor in the organism defence against oxidative stress. In this phase, we used an innovative technique of in vivo imaging that allowed us to follow the experimental subject in time and in vivo. reducing significantly animal stress. Secondly, an evaluation of different oxidative and inflammatory markers was carried on post-weaning piglets fed with melon pulp concentrate. The double aim of this trial was to validate melon pulp concentrate as a feed additives to improve immune response in challenged pigs and to monitor the trends of commonly used markers during LPS challenge. Then, as the effectiveness of new feed additives are under investigation and their effect on intestinal health need to be demonstrated, is essential to turn attention to intestinal health biomarkers, non- or minimally invasive. For this reason, the main aim of the last study was to develop and validate a new sandwich ELISA test for the quantification of Pacreatitis Associated Protein in pig faecal samples. Finally, an observational study on a new Oxidative Stress Index (OSi) was conducted on dairy cows during the transition period. The driving hypothesis was that a composed index, as OSi, predicts more accurately the oxidative status than the evaluation of reactive oxygen species or serum antioxidant capacity separately. Furthermore, the relationship between indexes of oxidative status and markers of energy balance as blood free fatty acids and β-hydroxybutyrate, was determined. Advisors/Committee Members: tutor: G. Savoini, coordinatore: F. Gandolfi, SAVOINI, GIOVANNI, GANDOLFI, FULVIO.

Subjects/Keywords: Settore AGR/18 - Nutrizione e Alimentazione Animale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mariani, E. (2019). NUTRITION AS A TOOL TO MODULATE PRODUCTION ANIMAL HEALTH. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/626377

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mariani, E.. “NUTRITION AS A TOOL TO MODULATE PRODUCTION ANIMAL HEALTH.” 2019. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/626377.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mariani, E.. “NUTRITION AS A TOOL TO MODULATE PRODUCTION ANIMAL HEALTH.” 2019. Web. 24 Jan 2021.

Vancouver:

Mariani E. NUTRITION AS A TOOL TO MODULATE PRODUCTION ANIMAL HEALTH. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/626377.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mariani E. NUTRITION AS A TOOL TO MODULATE PRODUCTION ANIMAL HEALTH. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/626377

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. G. Curone. PHYSIOLOGICAL APPROACH TO THE STUDY OF AUTOCHTHONOUS CATTLE BREEDS OF NORTHERN ITALY.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/625674

► This PhD project is focused on the physiological characterization of some autochthonous bovine breeds of Northern Italy, using many biological parameters in order to make… (more)

▼ This PhD project is focused on the physiological characterization of some autochthonous bovine breeds of Northern Italy, using many biological parameters in order to make a comparison with the cosmopolitan breeds. Despite the lower milk production, these breeds present other interesting and peculiar features, such as rusticity, frugality, good food conversion, high product quality (milk and meat), good fertility and good resistance and resilience against the diseases. We applied a multidisciplinary approach to study the physiological bases of the interesting traits present in the local breeds, studying three main issues: the metabolism of the transition period, the milk composition and technological properties and the ethological traits. a) Biodiversity of the transition period i) Study of the milk ketone bodies assessment in Modenese breed and Holstein with a characterization of physiological, reproductive and productive performances. The objective of this study was to characterize some productive, reproductive and metabolic parameters (ketone bodies) in the Italian autochthonous cattle breed Modenese, comparing them with those of Holstein and their crossbred (F1=Modenese x Holstein; F2=Modenese x F1) breed in the same farm, in order to understand if there is a different metabolic picture that can influence the reproductive performances. Milk samples have been collected at different times of lactation (20, 40, and 90 days in milk (DIM)) and analyzed by gas chromatography-mass spectrometry to obtain the the concentration of ketone bodies. In all time points, the Modenese breed showed a significant (p<0,05) lower concentration of ketone bodies. The F1, F2 and Modenese showed also better reproductive performances when compared to Holstein, with a significant lower (p<0,05) length of days open period. ii) Comparative evaluation of the transition period: Italian autochthonous breed vs. Holstein We evaluated the metabolic indicators, milk protein profiles and the milk microbiota in 6 Holstein Friesian and 4 Rendena cows reared on the same farm and under the same management conditions. Quarter milk samples and blood were collected from all cows at dry-off (T1), 1 day after calving (T2), 7-10 days after calving (T3) and 30 days after calving (T4). Blood samples were used for the analysis of plasma metabolites such as: glucose, total cholesterol, urea, inorganic phosphorus, total protein, albumin, total bilirubin, aspartate aminotransferase (GOT), γ-glutamyltransferase (GGT), creatinine, NEFA, β-OH-butyric acid (BHBA), thiol groups (SHp) and ferric reducing antioxidant power (FRAP). Quarter milk samples were subjected to bacteriological culture, protein profiles and characterization of the milk microbiota. Holstein cows showed a more severe fat mobilization, lower muscle mobilizationand systemic inflammatory response at T2 and T3 in comparison with Rendena cows. Significant differences were seen in the general composition of the milk microbiota of the two breeds. Concerning the milk protein abundance profile, pronounced… Advisors/Committee Members: tutor: D. Vigo, coordinatore: F. Gandolfi, VIGO, DANIELE, GANDOLFI, FULVIO.

Subjects/Keywords: biodiversity; bovine autochthonous breeds; microbiota; immune response; metabolic stress; dairy cow physiology; Settore VET/02 - Fisiologia Veterinaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Curone, G. (2019). PHYSIOLOGICAL APPROACH TO THE STUDY OF AUTOCHTHONOUS CATTLE BREEDS OF NORTHERN ITALY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/625674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Curone, G.. “PHYSIOLOGICAL APPROACH TO THE STUDY OF AUTOCHTHONOUS CATTLE BREEDS OF NORTHERN ITALY.” 2019. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/625674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Curone, G.. “PHYSIOLOGICAL APPROACH TO THE STUDY OF AUTOCHTHONOUS CATTLE BREEDS OF NORTHERN ITALY.” 2019. Web. 24 Jan 2021.

Vancouver:

Curone G. PHYSIOLOGICAL APPROACH TO THE STUDY OF AUTOCHTHONOUS CATTLE BREEDS OF NORTHERN ITALY. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/625674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Curone G. PHYSIOLOGICAL APPROACH TO THE STUDY OF AUTOCHTHONOUS CATTLE BREEDS OF NORTHERN ITALY. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/625674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. F. DI CESARE. CLASSICAL AND POPULATION PHARMACOKINETICS OF ANTIMICROBIALS, SEDATIVES AND ANAESTHETICS IN VETERINARY MEDICINE.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/625676

► Pharmacokinetics is defined as the use of mathematical models to quantitate the time course of drug absorption and disposition in man and animals. This discipline… (more)

▼ Pharmacokinetics is defined as the use of mathematical models to quantitate the time course of drug absorption and disposition in man and animals. This discipline is the branch of pharmacology that aims to describe, through mathematics, the interactions and physiological processes (i.e. absorption, distribution, metabolism and excretion – the ADME process) that the drugs undergo after administration. When applied to a clinical situation, pharmacokinetics (PK) provides the clinician with important information on optimal drug dosages for each single patient. In the present thesis, the focus moved to the importance of classical PK and its applications, starting from the theory and use of compartmental analysis and at the end arriving to illustrate the more recent notions of this discipline, popPK and PBPK, which are the most innovative topics in the clinical and translational pharmacology field. The classical PK usually, translated clinically, is represented by quick studies which have a significant relevance in understanding ADME process of drugs with their desired, collateral or adverse effects. The population PK approach aims to investigate the influence of the interindividual variability in a target population of subjects, evaluating the populations characteristics that influence the fate of a drug after administration. The time required for the development of a popPK study applied to the clinical setting is much longer than a classical PK study. Three studies of classical clinical PK and one population PK study were included in this thesis. The first study concerned dexmedetomidine (DEX) and aimed to define the kinetic profile of this sedative following intravenous administration in a group of dairy calves, comparing its pharmacological and clinical effects with those of another a-2 agonist, xylazine, for minor surgical procedures. The second study was related to the simultaneous administration, as preanaesthetics, of a mixture of DEX and methadone, an analgesic with a remarkable sedative efficacy belonging to the class of opioid μ-agonists. The aim was to establish the pharmacokinetic profile of this co-administration in dogs by oral transmucosal route and compare it with the intramuscular kinetic profile of the same drugs combination. The third research addressed the species Panthera tigris. Since the literature concerning the non- domestic animals’ PK is lacking and needy of new information, specifically, the objective was to compare the kinetic profile of a simultaneous administration of DEX and ketamine, an injectable anaesthetic antagonist of NMDA receptors, following IM administration for chemical restraint in two groups of tigers. The population PK study wanted to determine the popPK profile of cefazolin administered in a clinical setting for prophylactic purposes in 78 dogs, of different breed, age, weight, sex, body condition scores and health status, undergoing different surgical procedures. The ultimate goal was the definition of Clinical Breakpoints for this antimicrobial administered to the canine… Advisors/Committee Members: tutor: R.E. Villa, coordinatore: F. Gandolfi, VILLA, ROBERTO EDOARDO, GANDOLFI, FULVIO.

Subjects/Keywords: classical pharmacokinetics; population pharmacokinetics; antimicrobials; sedatives; anaesthetics; veterinary medicine; Settore VET/07 - Farmacologia e Tossicologia Veterinaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

CESARE, F. D. (2019). CLASSICAL AND POPULATION PHARMACOKINETICS OF ANTIMICROBIALS, SEDATIVES AND ANAESTHETICS IN VETERINARY MEDICINE. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/625676

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

CESARE, F. DI. “CLASSICAL AND POPULATION PHARMACOKINETICS OF ANTIMICROBIALS, SEDATIVES AND ANAESTHETICS IN VETERINARY MEDICINE.” 2019. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/625676.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

CESARE, F. DI. “CLASSICAL AND POPULATION PHARMACOKINETICS OF ANTIMICROBIALS, SEDATIVES AND ANAESTHETICS IN VETERINARY MEDICINE.” 2019. Web. 24 Jan 2021.

Vancouver:

CESARE FD. CLASSICAL AND POPULATION PHARMACOKINETICS OF ANTIMICROBIALS, SEDATIVES AND ANAESTHETICS IN VETERINARY MEDICINE. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/625676.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

CESARE FD. CLASSICAL AND POPULATION PHARMACOKINETICS OF ANTIMICROBIALS, SEDATIVES AND ANAESTHETICS IN VETERINARY MEDICINE. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/625676

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. C. Porcario. CHARACTERIZATION OF BETA AMYLOID DEPOSITION PROCESS IN THE BOVINE BRAIN: NEUROPATHOLOGICAL, IMMUNOBIOCHEMICAL AND GENETIC FEATURES.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/216691

► Senile and diffuse beta-amyloid (Aβ) plaques represent a characteristic trait of brain aging in both humans and animals. Regarding the latter, a wide range of… (more)

▼ Senile and diffuse beta-amyloid (Aβ) plaques represent a characteristic trait of brain aging in both humans and animals. Regarding the latter, a wide range of species, including non-human primates, the dog, the cat, the bear, the wolverine and the camel have been investigated for the presence of cerebral Aβ deposits. However, studies on the presence of Aβ deposits in the bovine brain are still lacking. Aim of this study was to characterize the hallmarks of neurodegeneration in the bovine brain by determining presence, distribution and deposition pattern of Aβ deposits by immunohistochemistry (IHC) and western blot (WB) techniques. Furthermore, the influence of apolipoprotein E (APOE) gene on Aβ-deposition process was also investigated. Formalin-fixed and frozen samples of four brain regions (cerebral cortex, hippocampus, cerebellum and brainstem) obtained from 102 cows (50 healthy and 52 diseased cases) ranging from fetuses to cattle of 240 months of age were examined. Intracellular or glia-associated Aβ deposits were observed by IHC, but a coexistence of these two patterns was often present, particularly in aged cattle. WB analysis showed the deposition of Aβ fragments Aβ 1-38, 1-40, 1-42 and 3-42 in the cerebral cortex and cerebellum. At both IHC and WB analysis the brainstem always appeared spare of Aβ deposits. The genetic variability of the bovine APOE gene was also characterized, assessing that that codons 112 and 158 of the APOE gene are not polymorphic in cattle, contrary to humans. However, nine polymorphisms of the APOE gene were detected, three of which not yet reported in GenBank. Interestingly, a polymorphism at position 876 related to the presence of an extracellular Aβ deposition pattern at the cerebral cortex level was detected. This study demonstrated the presence of Aβ peptides in the bovine brain at different ages and characterized the phenotype of Aβ distribution and deposition pattern. As this study disclosed similar mechanisms of protein aggregation during brain aging in both man and animals, it is conceivable that the bovine brain represents a valid animal model to understand the pathogenic mechanisms of neurodegeneration. Advisors/Committee Members: tutor: G. Poli, C. Casalone, coordinatore: F. Gandolfi, POLI, GIORGIO, GANDOLFI, FULVIO.

Subjects/Keywords: beta amyloid; bovine; brain; immunohistochemistry; immunofluorescence; western blot; genetics; Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Porcario, C. (2013). CHARACTERIZATION OF BETA AMYLOID DEPOSITION PROCESS IN THE BOVINE BRAIN: NEUROPATHOLOGICAL, IMMUNOBIOCHEMICAL AND GENETIC FEATURES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/216691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Porcario, C.. “CHARACTERIZATION OF BETA AMYLOID DEPOSITION PROCESS IN THE BOVINE BRAIN: NEUROPATHOLOGICAL, IMMUNOBIOCHEMICAL AND GENETIC FEATURES.” 2013. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/216691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Porcario, C.. “CHARACTERIZATION OF BETA AMYLOID DEPOSITION PROCESS IN THE BOVINE BRAIN: NEUROPATHOLOGICAL, IMMUNOBIOCHEMICAL AND GENETIC FEATURES.” 2013. Web. 24 Jan 2021.

Vancouver:

Porcario C. CHARACTERIZATION OF BETA AMYLOID DEPOSITION PROCESS IN THE BOVINE BRAIN: NEUROPATHOLOGICAL, IMMUNOBIOCHEMICAL AND GENETIC FEATURES. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/216691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Porcario C. CHARACTERIZATION OF BETA AMYLOID DEPOSITION PROCESS IN THE BOVINE BRAIN: NEUROPATHOLOGICAL, IMMUNOBIOCHEMICAL AND GENETIC FEATURES. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/216691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. G. Manarolla. SET-UP AND APPLICATION OF BIOTECHNOLOGICAL METHODS IN AVIAN FIELD.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/216693

► The use of biotechnology in avian field has been increased in the last decade especially on the track of the complete sequencing of chicken genome… (more)

▼ The use of biotechnology in avian field has been increased in the last decade especially on the track of the complete sequencing of chicken genome and on the global threat of avian flu pandemic which alerted the scientific community worldwide. Nevertheless, it is still on the whole a minor and spotty approach in avian field. Matter-of-factly, birds can be roughly divided into two groups on a research point of view: poultry and non-poultry (companion and wild birds). The economic impact of the latter is usually scarce if compared to the former and to other veterinary and human contexts. Thus, routine use of laboratory diagnostic tools including biotechnological methods is usually limited to the more expensive psittacines species (i.e. DNA-based determination of sex, diagnosis of chlamydiosis etc.) or to endangered species. As for poultry, extensive use of biotechnology is performed in vaccine research and genetic selection with a secondary role in avian and comparative pathology. Besides, chicken has historically assumed the role of avian model. Consequently, birds are often considered an uniform class but they are not, and a deep knowledge of the huge diversity which can exist among the several avian species is fundamental to correctly approach and interpret avian research no matter using biotechnology or not. For all these reasons it seemed an unique occasion to match the documented experience and activity in avian field (acquired during a previous doctorate) to biotechnological methods as the subject of this doctoral thesis. After a brief introduction revising fundamental principles of the molecular methods used, this thesis is divided into two parts. The first is focused on avian immunity and in particular on the TIR8-SIGIRR receptor which has been thoroughly investigate in chickens as a model for avian species and with an importance in itself as an ubiquitously diffused food production animal. The second part is made up of some diagnostic challenges arisen during the routine diagnostic activity of the Section of Avian Pathology of the Department of Veterinary Science and Public Health. Advisors/Committee Members: coordinatore: F. Gandolfi, tutor: V. Bronzo, supervisore: F. Riva, L. Turin, BRONZO, VALERIO, GANDOLFI, FULVIO.

Subjects/Keywords: Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Manarolla, G. (2013). SET-UP AND APPLICATION OF BIOTECHNOLOGICAL METHODS IN AVIAN FIELD. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/216693

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Manarolla, G.. “SET-UP AND APPLICATION OF BIOTECHNOLOGICAL METHODS IN AVIAN FIELD.” 2013. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/216693.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Manarolla, G.. “SET-UP AND APPLICATION OF BIOTECHNOLOGICAL METHODS IN AVIAN FIELD.” 2013. Web. 24 Jan 2021.

Vancouver:

Manarolla G. SET-UP AND APPLICATION OF BIOTECHNOLOGICAL METHODS IN AVIAN FIELD. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/216693.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Manarolla G. SET-UP AND APPLICATION OF BIOTECHNOLOGICAL METHODS IN AVIAN FIELD. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/216693

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. H. AHMED HUSSEIN AHMED. PROTEOMICS OF DIFFERENT CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/216649

► S. aureus causes severe infection even if it is mainly a harmless bacterium. It exists in a oxacillin-resistant (meticillin-resistant S. aureus, MRSA) form that represents… (more)

Subjects/Keywords: meticillin-resistant S. aureus; meticillin-sensible S. aureus; proteomics; Panton-Valentine leukocidin; iron metabolism; oxidative stress; Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

AHMED, H. A. H. (2013). PROTEOMICS OF DIFFERENT CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/216649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

AHMED, H. AHMED HUSSEIN. “PROTEOMICS OF DIFFERENT CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS.” 2013. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/216649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

AHMED, H. AHMED HUSSEIN. “PROTEOMICS OF DIFFERENT CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS.” 2013. Web. 24 Jan 2021.

Vancouver:

AHMED HAH. PROTEOMICS OF DIFFERENT CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/216649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

AHMED HAH. PROTEOMICS OF DIFFERENT CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/216649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. L. Restelli. MOLECULAR BASIS OF THE INNATE IMMUNE RESPONSE IN RUMINANTS: FOCUS ON ADIPOSE TISSUE.

Degree: 2014, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/230861

► The main aim of this thesis is to explore new aspects of the innate immune response in ruminants, especially focusing on the role of adipose… (more)

▼ The main aim of this thesis is to explore new aspects of the innate immune response in ruminants, especially focusing on the role of adipose tissue. Particularly, adipose tissue was investigated in order to provide new information in a species where this tissue is very poorly characterised. In ruminants, fat tissues play important biological roles for animal health for quality and gain in meat and milk production as well. A specific knowledge of how these pathways are controlled is of key importance for the management of animal health and from an economical perspective. Nevertheless, existing studies are mostly carried out in humans, where obesity is a major issue and little is known about ruminants. In this thesis, the distribution of white and brown adipose tissue in several adipose deposits was investigated through UCP1 expression and general histology, showing a clear distinction between these two macroareas, with brown adipose tissue mostly present in visceral deposits. With our study, we also demonstrated the presence of brown adipose tissue in 30 days old goat kids and therefore the presence of this tissue in growing animals and not only in newborns. In addition, visceral and subcutaneous deposits were investigated with proteomic techniques, demonstrating that these two macroareas can be clearly distinguished by their proteomic profiles, but single deposits within the same macroarea do not display particular proteomic differences. Moreover, we demonstrated the involvement of adipose tissue of goat kids in inflammatory and immune response pathways, through expression of at least 27 immune related proteins, of which nine, namely ceruloplasmin, gamma fibrinogen, hemopexin, kininogen 1, lactoferrin, protein dj, thiosulfate sulfurtransferase, tumour translationally controlled 1 and valacyclovir hydrolase, were never investigated before in adipose tissue. At a later stage, we focused our attention only on visceral adipose tissue, particularly on omentum, demonstrating that maternal diets enriched with either saturated or unsaturated fatty acid influence goat kid omentum proteome, but these influence is not confirmed at mRNA expression level. In addition, no influence of the maternal diet is showed on BAT distribution in goat kids. Advisors/Committee Members: tutor: V. Bronzo, supervisore: F. Ceciliani, coordinatore: F. Gandolfi, BRONZO, VALERIO, CECILIANI, FABRIZIO, GANDOLFI, FULVIO.

Subjects/Keywords: visceral adipose tissue; subcutaneous adipose tissue; brown adipose tissue; uncoupling protein 1; proteomics; maternal diet; goat; Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria; Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Restelli, L. (2014). MOLECULAR BASIS OF THE INNATE IMMUNE RESPONSE IN RUMINANTS: FOCUS ON ADIPOSE TISSUE. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/230861

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Restelli, L.. “MOLECULAR BASIS OF THE INNATE IMMUNE RESPONSE IN RUMINANTS: FOCUS ON ADIPOSE TISSUE.” 2014. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/230861.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Restelli, L.. “MOLECULAR BASIS OF THE INNATE IMMUNE RESPONSE IN RUMINANTS: FOCUS ON ADIPOSE TISSUE.” 2014. Web. 24 Jan 2021.

Vancouver:

Restelli L. MOLECULAR BASIS OF THE INNATE IMMUNE RESPONSE IN RUMINANTS: FOCUS ON ADIPOSE TISSUE. [Internet] [Thesis]. Università degli Studi di Milano; 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/230861.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Restelli L. MOLECULAR BASIS OF THE INNATE IMMUNE RESPONSE IN RUMINANTS: FOCUS ON ADIPOSE TISSUE. [Thesis]. Università degli Studi di Milano; 2014. Available from: http://hdl.handle.net/2434/230861

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. E. Razzuoli. PIG TONSIL CELLS AS A MODEL TO EVALUATE ORAL, LOW-DOSE CYTOKINE TREATMENTS.

Degree: 2014, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/230862

► Cytokines are low molecular weight mediators of the immune system. Because of their properties and biological roles, they have been used as diagnostic reagents for… (more)

▼ Cytokines are low molecular weight mediators of the immune system. Because of their properties and biological roles, they have been used as diagnostic reagents for human and animal diseases, as well as for prognostic tests and prophylactic treatments. Concerning this kind of treatments, there is uncertainty as to how cytokines are effective after oral administration. They probably act on the oral lymphoid tissues (palatine and pharyngeal tonsils) and trigger a cascade of events leading to activation of the immune system and control of the inflammatory cascade in tissues and organs. Owing to above, the aim of our project was to develop a reliable in vitro model related to the crucial interactions between cytokines and oral lymphoid cells, in terms of homeostatic regulation of the inflammatory response and antibody production. In this project, we focused our attention on the IFN-α system. The study was divided into 4 steps: 1) Isolation and culture of pig tonsil lymphocytes; 2) evaluation of IPEC-J2 cells as a reporter system of the anti-inflammatory control actions of interferon-alpha; 3) A pig tonsil cell culture model for evaluating oral, low-dose IFN-α treatments; 4) Disease-dependent modulation of tonsil cell phenotypes. In the first steps we develop reliable procedures for isolation and culture of pig tonsil cells, which were validated for use in functional immunoassays. Our results indicate that pig tonsil cells can be employed within 2 months from freezing to maintain suitable conditions in terms of recovery, vitality and release of antibody in vitro. Tonsil mononuclear cells also showed the ability to secrete antimicrobial peptides and to respond in vitro to immunological stimuli. In the second part of the project, IPEC-J2 (a continuous cell line of porcine intestinal epithelial cells) was validated as reporter system of the biological properties of IFN-α. Three different experimental conditions (oxidative stress, inflammatory response, and amplification of lymphoid cell signals) were selected to evaluate the effects of porcine recombinant IFN-α1 (rIFN-α) and 2 natural porcine IFN-α preparations (nIFN-α). The IFNs under study showed significantly different control actions in IPEC-J2 cells. In particular, rIFN-α was shown to down-regulate interleukin (IL)-8, IL-1β, tumor necrosis factor (TNF)-α, and β-defensin 1 genes either directly, or indirectly through second messengers released by IFN-α-treated lymphoid cells. With regard to IL-6, only second messengers from IFN-α-treated lymphoid cells could regulate the expression of this cytokine. Our results indicate that IPEC-J2 cells can be a useful tool for investigating the regulatory actions of type I IFNs and second messengers thereof. In the third step we developed an in vitro model of interaction between different types of human and porcine IFNs-α at low / moderate concentrations and pig tonsil cells. The IFNs-α under study showed different properties with respect to three fundamental control actions: 1) IgA release in culture (up and down-regulation,… Advisors/Committee Members: tutor: L. Bonizzi, M. Amadori, coordinatore: F. Gandolfi, BONIZZI, LUIGI, GANDOLFI, FULVIO.

Subjects/Keywords: pig; tonsil; lymphocytes; IPEC; citokines; IL-8; IL-6; TNF-Alpha; Type I IFNs; Settore VET/05 - Malattie Infettive degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Razzuoli, E. (2014). PIG TONSIL CELLS AS A MODEL TO EVALUATE ORAL, LOW-DOSE CYTOKINE TREATMENTS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/230862

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Razzuoli, E.. “PIG TONSIL CELLS AS A MODEL TO EVALUATE ORAL, LOW-DOSE CYTOKINE TREATMENTS.” 2014. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/230862.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Razzuoli, E.. “PIG TONSIL CELLS AS A MODEL TO EVALUATE ORAL, LOW-DOSE CYTOKINE TREATMENTS.” 2014. Web. 24 Jan 2021.

Vancouver:

Razzuoli E. PIG TONSIL CELLS AS A MODEL TO EVALUATE ORAL, LOW-DOSE CYTOKINE TREATMENTS. [Internet] [Thesis]. Università degli Studi di Milano; 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/230862.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Razzuoli E. PIG TONSIL CELLS AS A MODEL TO EVALUATE ORAL, LOW-DOSE CYTOKINE TREATMENTS. [Thesis]. Università degli Studi di Milano; 2014. Available from: http://hdl.handle.net/2434/230862

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. R. Pasquariello. MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE.

Degree: 2015, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/335298

► My PhD project addressed the paternal and maternal contribution associated with correct early embryonic development, focusing on microRNAs (miRNAs, short non-coding RNAs regulating gene expression… (more)

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pasquariello, R. (2015). MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/335298

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pasquariello, R.. “MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE.” 2015. Thesis, Università degli Studi di Milano. Accessed January 24, 2021. http://hdl.handle.net/2434/335298.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pasquariello, R.. “MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE.” 2015. Web. 24 Jan 2021.

Vancouver:

Pasquariello R. MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE. [Internet] [Thesis]. Università degli Studi di Milano; 2015. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2434/335298.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pasquariello R. MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE. [Thesis]. Università degli Studi di Milano; 2015. Available from: http://hdl.handle.net/2434/335298

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations